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To screen and identify high chitosanase producing strains and to optimize the fermentation condition and analyze the enzymatic properties of the target strain. In this study, high chitosanase-producing strain was screened from the sediment of shrimp ponds in Beihai, Guangxi through primary and secondary screening. The strain underwent identification, the enzyme production process of the strain was optimized through single-factor, Plackett-Burman and orthogonal optimization methods. The enzymatic properties and products of chitosanase produced by the strain were studied. The results showed that the strain of Gxun-5.3.3 with high chitosanase production was obtained by screening and was identified as Bacillus cereus. The optimal components for the enzyme-producing medium of B. cereus Gxun-5.3.3 was maltose 5 g/L, yeast powder 3 g/L, chitosan 15 g/L, and the initial pH and fermentation time were 6.0 and 84 h, respectively. Under the optimized conditions, the chitosanase activity reached 62.03±1.18 U/mL, which was 55.58% higher before optimization (39.87±1.24 U/mL). The protease produced by strain Gxun-5.3.3 had maximum activity at 60 ℃ and optimum pH at 5.0, respectively. Metal ions Fe2+ and Mn2+ had a significant (P

Abstract The exceptional optoelectronic properties of lead halide perovskite nanocrystals (PeNCs) in the ultraviolet and visible spectral regions have positioned them as a promising class of semiconductor materials for diverse optoelectronic and photovoltaic applications. However, their limited response to near‐infrared (NIR) light due to the intrinsic bandgap (>1.5 eV) has hindered their applications in many advanced technologies. To circumvent this limitation, it is of fundamental significance to integrate PeNCs with lanthanide‐doped upconversion nanoparticles (UCNPs) that are capable of efficiently converting low‐energy NIR photons into high‐energy ultraviolet and visible photons. By leveraging the energy transfer from UCNPs to PeNCs, this synergistic combination can not only expand the NIR responsivity range of PeNCs but also introduce novel emission profiles to upconversion luminescence with multi‐dimensional tunability (e.g., wavelength, lifetime, and polarization) under low‐to‐medium power NIR irradiation, which breaks through the inherent restrictions of individual PeNCs and UCNPs and thereby opens up new opportunities for materials and device engineering. In this review, we focus on the latest advancements in the development of PeNCs‐UCNPs nanocomposites, with an emphasis on the controlled synthesis and optical properties design for advanced optoelectronic applications such as full‐spectrum solar cells, NIR photodetectors, and multilevel anticounterfeiting. Some future efforts and prospects toward this active research field are also envisioned.

IntroductionFusarium wilt of banana, also recognized as Panama disease, is caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense tropical race 4 (FOC TR4). In recent years, strategies utilizing biocontrol agents, comprising antifungal microorganisms and their associated bioactive compounds from various environments, have been implemented to control this destructive disease. Our previous study showed that Pseudomonas aeruginosa Gxun-2 had significant antifungal effects against FOC TR4. However, there has been little scientific investigation of the antibacterial or antifungal activity. The aim of this study was to isolate, identify and evaluate the inhibition strength of active compounds in P. aeruginosa Gxun-2, so as to explain the mechanism of the strain inhibition on FOC TR4 from the perspective of compounds.MethodsThe main antibacterial compounds of strain Gxun-2 were isolated, purified and identified using by fermentation extraction, silica gel column chromatography, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) techniques. The effect of the compounds on the mycelial growth, morphology and spore germination of strain FOC TR4 was observed by 96-well plate method and AGAR diffusion method.ResultsAmong the metabolites produced by the strain, four antifungal compounds which were identified phenazine (C12H8N2), phenazine-1-carboxylic acid (PCA) (C13H8N2O2), 2-acetamidophenol (C8H9NO2) and aeruginaldehyde (C10H7NO2S) were identified through HPLC and NMR. Of these compounds, phenazine and PCA exhibited the most pronounced inhibitory effects on the spore germination and mycelial growth of FOC TR4. Phenazine demonstrated potent antifungal activity against FOC TR4 with a minimum inhibitory concentration (MIC) of 6.25 mg/L. The half-maximal effective concentration (EC50) was calculated to be 26.24 mg/L using the toxicity regression equation. PCA exhibited antifungal activity against FOC TR4 with an MIC of 25 mg/L and an EC50 of 89.63 mg/L. Furthermore, phenazine and PCA triggered substantial morphological transformations in the mycelia of FOC TR4, encompassing folding, bending, fracturing, and diminished spore formation.DiscussionThese findings indicate that strain Gxun-2 plays a crucial role in controlling FOC TR4 pathogenesis, predominantly through producing the antifungal compounds phenazine and PCA, and possesses potential as a cost-efficient and sustainable biocontrol agent against Fusarium wilt of banana in forthcoming times.

The mining activation of hidden faults under dynamic and static loads is an important reason for the occurrence of floor water inrush disasters in deep coal seam mining. The formation and evolution mechanism of water inrush channel caused by mining on the floor of hidden faults were analyzed through numerical simulation, from the perspective of fracture mechanics, a model was constructed to explore the influence of combined dynamic and static loads on the propagation of water with cracks. A conclusion was drawn that the effects of mining stress and confined water have led to rapid expansion of hidden fault cracks and significant improvement in permeability, at the same time, the confined water in the hidden fault also has a scouring and expansion effect on the cracks, accelerating their development speed. There are spatial and temporal differences in the penetration patterns of hidden faults at different positions of floor, and the closer it is to the goaf, the more likely it is to experience activation of hidden faults and water inrush. When there are multiple hidden small faults in the floor, there is an alternating change between the water inrush growth area and the flow stable area with similar cyclic characteristics. The effect of dynamic load will increase the pore pressure in cracks, and increase the stress intensity factor at the crack tip, and more easily induce crack expansion and penetration failure. The critical water pressure calculation equation for crack propagation and failure under dynamic and static loads was derived, and the calculation method for the minimum safe thickness of the floor was further analyzed, the influence of water pressure, crack length, inclination angle, and mining depth on it was discussed. The effect of dynamic load will increase the pore pressure in cracks, and increase the stress intensity factor at the crack tip, and more easily induce crack expansion and penetration failure. Finally, the theoretical analysis results were verified by an engineering examples. The research results can provide theoretical basis for predicting and preventing water inrush from the mining floor, which is beneficial for the safe and sustainable mining of coal mines.

IntroductionChitin, abundant in marine environments, presents significant challenges in terms of transformation and utilization. A strain, T22.7.1T, with notable chitin deacetylation capabilities, was isolated from the rhizosphere of Acanthus ebracteatus in the North Sea of China. Comparative 16S rDNA sequence analysis showed that the new isolate had the highest sequence similarity (99.79%) with Rhodococcus indonesiensis CSLK01-03T, followed by R. ruber DSM 43338T, R. electrodiphilus JC435T, and R. aetherivorans 10bc312T (98.97%, 98.81%, and 98.83%, respectively). Subsequent genome sequencing and phylogenetic analysis confirmed that strain T22.7.1T belongs to the R. indonesiensis species. However, additional taxonomic characterization identified strain T22.7.1T as a novel type strain of R. indonesiensis distinct from CSLK01-03T.MethodsThis study refines the taxonomic description of R. indonesiensis and investigates its application in converting chitin into chitosan. The chitin deacetylase (RiCDA) activity of strain T22.7.1T was optimized, and the enzyme was isolated and purified from the fermentation products.ResultsThrough optimization, the RiCDA activity of strain T22.7.1T reached 287.02 U/mL, which is 34.88 times greater than the original enzyme’s activity (8.0 U/mL). The natural CDA enzyme was purified with a purification factor of 31.83, and the specific activity of the enzyme solution reached 1200.33 U/mg. RiCDA exhibited good pH and temperature adaptability and stability, along with a wide range of substrate adaptabilities, effectively deacetylating chitin, chitooligosaccharides, N-acetylglucosamine, and other substrates.DiscussionProduct analysis revealed that RiCDA treatment increased the deacetylation degree (DD) of natural chitin to 83%, surpassing that of commercial chitosan. Therefore, RiCDA demonstrates significant potential as an efficient deacetylation tool for natural chitin and chitooligosaccharides, highlighting its applicability in the biorefining of natural polysaccharides.

Abstract The authors show a PM motor drive with the maximum speed of 170,000 r/min for a super‐high‐speed centrifugal air compressor, which is used in high‐altitude unmanned aerial vehicle (UAV). Conventional turbochargers used in high‐altitude UAV are usually driven by the exhaust of the engine in the fuel cell system; however, the exhaust is composed of vapour and water drop, which can't drive a mechanical turbocharger effectively. In this circumstance, a super‐high‐speed electric centrifugal compressor becomes attractive to boost the air pressure and improve overall efficiency. A wide‐speed‐adaptive sliding mode observer (WSA‐SMO) is adopted to realise the super‐high‐speed sensorless control. A super‐high‐speed motor drive system is developed based on the WSA‐SMO. The current waveform at 170,000 r/min is shown to prove the steady state performance. The acceleration curves and start/stop curves are shown to demonstrate the excellent dynamic performances.

The rapidly developing additive Manufacturing (AM) technology could overcome the critical dimension limit of metallic glasses (MGs). This permits to obtain MG components with complex geometry and large dimensions. However, the residual defects, such as pores and cracks in AMed MGs, significantly deteriorate their performance. Thus, it is essential to suppress and eliminate the pores and cracks that remain after additive manufacturing.This paper critically reviews the pores and cracks in the laser powder bed fusion (LPBF) fabricated MGs, including recent achievements and challenges in depressing their initiation, alleviating their propagation, and eliminating their collapse. The recent advances in LPBFed MGs and the suppression strategies of pores and cracks, such as parameters optimization, scanning strategy alteration, second phase's introduction, substrate and MG system selection as well as components performance improvement with designed porosity, are reviewed and commented, especially for several specific MG systems. The pores and cracks initiation and alleviation mechanisms are further analyzed. Also, the unsolved issues, challenges, and future research directions are evaluated. Finally, concluding remarks and an outlook concerning the pores and cracks issues in LPBFed MGs are further provided.

Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze the oxidative cleavage of recalcitrant polysaccharides. There are limited reports on LPMOs capable of concurrently catalyzing the oxidative cleavage of both cellulose and chitin. In this study, we identified and cloned a novel LPMO from the newly isolated bacterium Chitinilyticum aquatile CSC-1, designated as CaLPMO10. When using 2, 6-dimethylphenol (2, 6-DMP) as the substrate, CaLPMO10 exhibited optimal activity at 50 °C and pH 8, demonstrating good temperature stability at 30 °C. Even after a 6 h incubation at pH 8 and 30 °C, CaLPMO10 retained approximately 83.03 ± 1.25% residual enzyme activity. Most metal ions were found to enhance the enzyme activity of CaLPMO10, with ascorbic acid identified as the optimal reducing agent. Mass spectrometry analysis indicated that CaLPMO10 displayed oxidative activity towards both chitin and cellulose, identifying it as a C1/C4-oxidized LPMO. CaLPMO10 shows promise as a key enzyme for the efficient utilization of biomass resources in future applications.

Abstract Background Lung adenocarcinoma (LUAD) is a common type of lung cancer with a high risk of metastasis, but the exact molecular mechanisms of metastasis are not yet understood. Methods This study acquired single-cell transcriptomics profiling of 11 distal normal lung tissues, 11 primary LUAD tissues, and 4 metastatic LUAD tissues from the GSE131907 dataset. The lung multicellular ecosystems were characterized at a single-cell resolution, and the potential mechanisms underlying angiogenesis and metastasis of LUAD were explored. Results We constructed a global single-cell landscape of 93,610 cells from primary and metastatic LUAD and found that IGF2BP2 was specifically expressed both in a LUAD cell subpopulation (termed as LUAD_IGF2BP2), and an endothelial cell subpopulation (termed as En_IGF2BP2). The LUAD_IGF2BP2 subpopulation progressively formed and dominated the ecology of metastatic LUAD during metastatic evolution. IGF2BP2 was preferentially secreted by exosomes in the LUAD_IGF2BP2 subpopulation, which was absorbed by the En_IGF2BP2 subpopulation in the tumor microenvironment. Subsequently, IGF2BP2 improved the RNA stability of FLT4 through m6A modification, thereby activating the PI3K-Akt signaling pathway, and eventually promoting angiogenesis and metastasis. Analysis of clinical data showed that IGF2BP2 was linked with poor overall survival and relapse-free survival for LUAD patients. Conclusions Overall, these findings provide a novel insight into the multicellular ecosystems of primary and metastatic LUAD, and demonstrate that a specific LUAD_IGF2BP2 subpopulation is a key orchestrator promoting angiogenesis and metastasis, with implications for the gene regulatory mechanisms of LUAD metastatic evolution, representing themselves as potential antiangiogenic targets.

Abstract Lanthanide (Ln3+) oxysulfide nanocrystals (NCs) have great prospect in many advanced technologies; however, they suffer from a low photoluminescence efficiency due to the volatility of sulfur and deleterious surface quenching effect. Herein, we report a novel sandwiched luminescent heterostructure based on Ln3+‐doped Gd2O2S@NaYF4 core/shell NCs with tunable sulfur content in the sandwich layer. By means of Eu3+ as the sensitive structural probes, we unravel the ligand‐mediated structure control of the NCs from Gd2O3: Ln3+@NaYF4 to Gd2O2S: Ln3+@NaYF4 with tailored S2– deficiency. Such a sandwich‐type core/shell heterostructure enables us to achieve efficient and multicolor downshifting and upconversion luminescence (UCL), with up to 208.8 folds of enhancement in UCL intensity as compared to that of their core‐only counterparts. These findings provide a general approach for the controlled synthesis of lanthanide oxysulfide@fluoride heterostructure, which offers a new way for the materials design towards diverse emerging applications.

Objective: Osteosarcoma (OS) is an uncommon sarcoma with osteoid formation in conjunction with malignantmesenchymal cells on histological examination. SP-8356 has been reported to exhibit anti-cancer properties in humancancers. However the impact of SP-8356 on OS is largely unknown. The metabolic pathways are coordinated by AMPactivatedprotein kinase (AMPK), which maintains a balance between the supply and demand of nutrients and energy.This study aimed to investigate effect of SP-8356 on proliferation and apoptosis of OS cells and tumor growth in mice.Furthermore, involvement of PGC-1α/TFAM and AMPK-activation was studied.Materials and Methods: In the experimental study, Saos-2 and MG63 cells were cultured with SP-8356 for 24hours and analysed for cellular proliferation using MTT assay. DNA fragmentation was studied using ELISA basedkit. Furthermore, transwell chambers assay was used to determine cell migration and cell invasion. Targeted proteinexpression levels were assessed using western blotting. For in vivo studies, mice (5-6 weeks old) were implanted witheither Saos-2 or MG63 cells on dorsal surface subcutaneously and they were administered with SP-8356 (10 mg/kg)for two weeks prior to bone tumor induction.Results: We found that SP-8356 exerted anti-proliferative effects on Saos-2 and MG63 cells. Furthermore, SP-8356treatment significantly restricted migration and invasion of Saos-2 and MG63 cells. Compared to the control, SP-8356significantly reduced apoptotic cell death, while it increased PGC-1α and TFAM expressions. Without affecting bodyweight, SP-8356 significantly reduced tumor development in mice, as compared to the control group.Conclusion: SP-8356 was found to inhibit proliferation, suppressed cells migration and invasion and decreased OStumor growth. Furthermore, SP-8356 was found to act through PGC-1α/TFAM and AMPK activations. SP-8356 can betherefore used as therapeutic agent for OS treatment.

Abstract The prevalence and mortality of hepatocellular carcinoma (HCC) are still increasing. This study aimed to identify potential therapeutic targets related to patient prognosis. Data were downloaded from TCGA, GSE25097, GSE36376, and GSE76427 datasets. Differential analysis and enrichment analysis were performed in HCC. Cell deaths were evaluated, and least absolute shrinkage and selection operator regression (LASSO) regression was analyzed to screen candidate genes. Additionally, immune cell infiltration in HCC was assessed. We identified 4088 common DEGs with the same direction of differential expression in all four datasets, they were mainly enriched in immunoinflammation and cell cycle pathways. Apoptosis was significantly suppressed in HCC in GSEA and GSVA. After LASSO regression analysis, we screened CD69, CDC25B, MGMT, TOP2A, and TXNIP as candidate genes. Among them, CD69 significantly influenced the overall survival of HCC patients in both TCGA and GSE76427. CD69 may be a protective factor for outcome of HCC patients. In addition, CD69 was positive correlation with T cells and CD3E. CD69, CDC25B, MGMT, TOP2A, and TXNIP were potential diagnostic and prognostic target for HCC, especially CD69.

This paper proposes a dynamic encircling cooperative guidance (DECG) law to enable multiple interceptors to cooperatively intercept a superior target, considering low velocity, limited overload, impact angle and simultaneous arrival constraints. First, the feasible escaping area of the target is analyzed and a dynamic encircling strategy for the target is established. This strategy efficiently provides virtual escaping points, allowing interceptors to dynamically encircle the target without excessive energy consumption, ultimately leading to a successful interception. Second, to enhance the physical feasibility of the kinematic equations governing the interaction between interceptors and target at the virtual escaping points, the independent variable is substituted and the kinematic equations are remodeled. Convex optimization is employed to address the multi-constraint optimal guidance problem for each interceptor, thereby facilitating simultaneous interception. Compared with the existing guidance laws, DECG has a more practical and feasible cooperative strategy, is able to handle more constraints including the interceptor’s own constraints and cooperative constraints, and does not rely on the precise calculation of explicit remaining flight time in the guidance law implementation. Lastly, the effectiveness, superiority and robustness of the DECG law are evaluated through a series of numerical simulations, and its performance is compared with that of the cooperative proportional navigation guidance law (CPNG).

Microbial degradation of feathers offers potential for bioremediation, yet the microbial response mechanisms warrant additional investigation. In prior work, Pseudomonas aeruginosa Gxun-7, which demonstrated robust degradation of feathers at elevated concentrations, was isolated. However, the molecular mechanism of this degradation remains only partially understood. To investigate this, we used RNA sequencing (RNA-seq) to examine the genes that were expressed differentially in P. aeruginosa Gxun-7 when exposed to 25 g/L of feather substrate. The RNA-seq analysis identified 5571 differentially expressed genes; of these, 795 were upregulated and 603 were downregulated. Upregulated genes primarily participated in proteolysis, amino acid, and pyruvate metabolism. Genes encoding proteases, as well as those involved in sulfur metabolism, phenazine synthesis, and type VI secretion systems, were notably elevated, highlighting their crucial function in feather decomposition. Integration of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) taxonomies, combined with a review of the literature, led us to propose that metabolic feather degradation involves environmental activation, reducing agent secretion, protease release, peptide/amino acid uptake, and metabolic processes. Sulfite has emerged as a critical activator of keratinase catalysis, while cysteine serves as a regulatory mediator. qRT–PCR assay results for 11 selected gene subset corroborated the RNA-seq findings. This study enhances our understanding of the transcriptomic responses of P. aeruginosa Gxun-7 to feather degradation and offers insights into potential degradation mechanisms, thereby aiding in the formulation of effective feather waste management strategies in poultry farming.

Both environmental and genetic factors contribute to the etiology of autoimmune thyroid disease (AITD) including Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). However, the exact pathogenesis and interactions that occur between environmental factors and genes remain unclear, and therapeutic targets require further investigation due to limited therapeutic options. To solve such problems, this study utilized single-cell transcriptome, whole transcriptome, full-length transcriptome (Oxford nanopore technology), and metabolome sequencing to examine thyroid lesion tissues from 2 HT patients and 2 GD patients as well as healthy thyroid tissue from 1 control subject. HT patients had increased ATF4-positive thyroid follicular epithelial (ThyFoEp) cells, which significantly increased endoplasmic reticulum stress. The enhanced sustained stress resulted in cell death mainly including apoptosis and necroptosis. The ATF4-based global gene regulatory network and experimental validation revealed that N6-methyladenosine (m6A) reader hnRNPC promoted the transcriptional activity, synthesis, and translation of ATF4 through mediating m6A modification of ATF4. Increased ATF4 expression initiated endoplasmic reticulum stress signaling, which when sustained, caused apoptosis and necroptosis in ThyFoEp cells, and mediated HT development. Targeting hnRNPC and ATF4 notably decreased ThyFoEp cell death, thus ameliorating disease progression. Collectively, this study reveals the mechanisms by which microenvironmental cells in HT and GD patients trigger and amplify the thyroid autoimmune cascade response. Furthermore, we identify new therapeutic targets for the treatment of autoimmune thyroid disease, hoping to provide a potential way for targeted therapy.

The roof in a fully-mechanized face of a shallow coal seam with large mining height is prone to form a combined cantilever—articulated rock beam structure. When the support resistance is insufficient, the articulated rock beam will sink. This will make the combined cantilever beam rotate and fracture. It is then easy to induce the sliding and instability of the articulated rock beam, which results in large-scale roof cutting and the support crushing. Taking the combined cantilever beam structure as the main research object, and considering the mining damaged characteristics of cantilever beam rock stratum, the rock beam was regarded as a finite plate model with an edge crack of arbitrary dip angle. In addition, a fracture mechanics model controlled by a set of structural planes was established, the instability conditions of rock beam and the main control factors were analyzed, and the method of determining the support resistance were discussed. The results show that the cantilever beam rotates and fractures. This causes a chain reaction of the rock beam that leads to fracture. The combined cantilever beam then loses stability with the increase of the length of the crack length and the crack dip angle, and therefore it is easier to penetrate the cantilever beam and cause roof instability. The necessary condition for rock beam instability was crack activation, and the sufficient condition was the airfoil branch crack propagate through the rock beam. The influence degree of each parameter on the support resistance was thus determined: crack length a > crack dip angle β > rock thickness h > weighting interval l. The theoretical analysis results were proven to be reasonable by an in situ monitoring example of no. 22,310 working face in the Daliuta coal mine, China. On this basis, the reasonable value of support resistance was obtained. The conclusions of this research provide a new method for researching the roof instability mechanism. They are also conducive to the green and sustainable development of mines.

The antitumor drug doxorubicin is widely used in clinical practice. However, the low yield and high cost of this drug highlight the urgent need for cost-effective processes to rapidly manufacture antitumor drugs at scale. In the biosynthesis pathway, the multi-functional cytochrome P450 enzyme DoxA catalyzes the last three steps of hydroxylation. The final conversion of daunorubicin to doxorubicin is the rate-limiting step. In our work, the DoxA has been expressed with the ferredoxin reductase FDR2 and the ferredoxin FDX1 and purified to homogeneous. The reduced carbon monoxide difference spectroscopy, heme concentration, and enzymatic characteristic were characterized. These studies suggest an approach for engineering Streptomyces P450s with functional expression for mechanistic and structural studies.

N-acetylglucosamine (GlcNAc) is widely used in nutritional supplement and is generally produced from chitin using chitinases. While most GlcNAc is produced from colloidal chitin, it is essential that chitinases be acidic enzymes. Herein, we characterized an acidic, highly salinity tolerance and thermostable chitinase AfChiJ, identified from the marine fungus Aspergillus fumigatus df673. Using AlphaFold2 structural prediction, a truncated Δ30AfChiJ was heterologously expressed in E. coli and successfully purified. It was also found that it is active in colloidal chitin, with an optimal temperature of 45°C, an optimal pH of 4.0, and an optimal salt concentration of 3% NaCl. Below 45°C, it was sound over a wide pH range of 2.0–6.0 and maintained high activity (≥97.96%) in 1–7% NaCl. A notable increase in chitinase activity was observed of Δ30AfChiJ by the addition of Mg2+, Ba2+, urea, and chloroform. AfChiJ first decomposed colloidal chitin to generate mainly N-acetyl chitobioase, which was successively converted to its monomer GlcNAc. This indicated that AfChiJ is a bifunctional enzyme, composed of chitobiosidase and β-N-acetylglucosaminidase. Our result suggested that AfChiJ likely has the potential to convert chitin-containing biomass into high-value added GlcNAc.

FlAlyA, a PL7 alginate lyase with industrial potential, is widely applied in the preparation the alginate oligosaccharide because of its high activity of degradation the alginate. However, heat inactivation still limits the industrial application of FlAlyA. To further enhance its thermostability, a group of mutants were designed, according to evaluating the B-factor value and free energy change via computer-aided calculation. 25 single-point mutants and one double-points mutant were carried out by site-directed mutagenesis. The optimal two single-point mutants H176D and H71K showed 1.20 and 0.3°C increases in the values of Tm, while 7.58 and 1.73 min increases in the values of half-life (t1/2) at 50°C, respectively, compared with that of the wild-type enzyme. Interestingly, H71K exhibits the comprehensive improvement than WT, including expression level, thermal stability and specific activity. In addition, the mechanism of these two mutants is speculated by multiple sequence alignment, structural basis and molecular dynamics simulation, which is likely to be involved in the formation of new hydrogen bonds and decrease the SASA of the mutants. These results indicate that B-factor is an efficient approach to improves the thermostability of alginate lyase composed of β-sheet unit. Furthermore, the highest yield of the mutant reached about 650 mg/L, which was nearly 36 times that of previous studies. The high expression, excellent activity and good thermal stability make FlAlyA a potential candidate for the industrial production of alginate oligosaccharides.

Anthracyclines are an important class of natural antitumor drugs. They have a conservative aromatic tetracycline backbone that is substituted with different deoxyglucoses. The deoxyglucoses are crucial for the biological activity of many bacterial natural products after the proper modification from glycosyltransferases (GTs). The difficulty in obtaining highly purified active GTs has prevented biochemical studies on natural product GTs. In this paper, a new Escherichia coli fusion plasmid pGro7′, which introduces the Streptomyces coelicolor chaperone genes groEL1, groES and groEL2, was constructed. The glycosyltransferase DnmS from Streptomyces peucetius ATCC 27952 was co-expressed with the plasmid pGro7′, and unprecedented high-efficiency and soluble expression of DnmS in the E. coli expression system was realized. Subsequently, the reverse glycosylation reaction characteristics of DnmS and DnmQ were verified. We found that DnmS and DnmQ had the highest enzyme activity when they participated in the reaction at the same time. These studies provide a strategy for the soluble expression of GTs in Streptomyces and confirm the reversibility of the catalytic reaction of GTs. This provides a powerful method for the production of active anthracyclines and to enhance the diversity of natural products.

At present, two-stage centrifugal compressors based on air bearing and high-speed motor technology are widely used in automotive hydrogen fuel cells. The low-pressure stage and high-pressure stage of the compressor are directly connected through an interstage pipeline, thus the structure of the interstage pipeline has an important influence on the aerodynamic performance of the compressor. In this work, a two-stage compressor with three different interstage pipelines were investigated experimentally and numerically. Results show that affected by the interstage pipeline bend section, the flow distortion will be induced at the impeller inlet of the high-pressure stage, and the distortion intensity changes with the pipeline structure. Among the three models, the EPC (elbow pipe change) model induces the most intense total pressure distortion at the condition of 80 kr/min and 130% mass flow rate, resulting in an efficiency reduction of 2 and 1.5 percentage points compared with the SPC (straight pipe change) and the TPC (total pipe change) model, respectively. Further research indicates that the upstream distortion has obvious influence on the downstream rotor blades. As the blade height decreases, the load becomes more uniform on the main blades, while the load extremum migrates to the trailing edge on the splitter blades. Finally, three models are tested, and their performance is compared at three typical rotational speeds., It is recommended that interstage pipelines similar to SPC models should be chosen to improve the two-stage compressor efficiency in the design.

In order to effectively weaken the leakage flow and shock intensity of traditional “swing” type guide vanes in a variable nozzle turbine, a new flow control device named the “split sliding guide vane” (SSGV) is studied in the present work. Steady and unsteady calculations were carried out on both the SSGV and base model at 10%, 40%, and 100% open positions. The shock test was performed to verify the accuracy of the numerical method. The results showed that at 10%, 40%, and 100% open positions, the leakage flow of the SSGV was 43%, 51%, and 40% of that of the base model, respectively. When 10% open, the turbine efficiency increased by 12%, compared with the base model, since the SSGV could effectively inhibit the clearance leakage flow. Due to the increased distance between the rotor and guide vane, the shock intensity of the SSGV was only 52% of that of the base model when 40% was open. The SSGV could reduce the static pressure loss on the guide vane pressure surface, but for the guide vane suction surface, the static pressure distribution appeared in a “W” shape due to the influence of the vane profile. Finally, the flow in the rotor was studied, which showed that the weakening of the shock and reduction of the clearance leakage flow in the guide vane were also beneficial for the strength of downstream rotor blades.

Multi-walled carbon nanotubes (MWCNTs) were modified on the tips and inner walls by 12-chloro-12-oxododecanedioic acid-methyl ester groups and then added to the polyamide composite membranes to prepare MWCNT-CH2OCOC12H23O2 membranes for desalination. The characterization results of transmission electron microscopy, Fourier transform, infrared transform, and thermogravimetric analysis showed that the 12-chloro-12-oxododecanedioic acid-methyl ester group was successfully grafted to the entrances and inner walls of the MWCNTs. The performance of the MWCNTs’ composite membranes was evaluated by scanning electron microscopy, contact angle, and filtration test. The modified membrane morphology is more uniform, and there is no structural damage. The grafting of carbon nanotubes with methyl 12-chloro-12-oxydodecyldicarboxylate could improve the hydrophilicity of the membrane. Under identical conditions, the water flux of MWCNT-CH2OCOC12H23O2 membranes was higher than that of the pristine carbon nanotube’s membrane, and the desalination rate was also slightly improved.

To enhance the water flux and salt rejection of reverse osmosis membranes, thin film nanocomposite reverse osmosis membranes were prepared by incorporating functionalised single-walled carbon nanotubes (SWNTs). The functionalised SWNTs were obtained by chemical process such as mixed acid oxidation and substitution reaction. The SWNTs were characterised by HRTEM, FTIR, TGA, Raman spectra, UV-Vis and XPS, etc. The surface morphology and structure of membrane were characterised by SEM and contact angle measurement, respectively. The results showed that carboxyl, acyl, amide and amine were successfully grafted on the tip and inner wall of the single-walled carbon nanotubes. The dispersity of the functionalised SWNTs in water was tested, indicating a good hydrophilic property. The polyamide/modified CNT nanocomposite reverse osmosis membrane was prepared. Compared with the bare polyamide membrane, the SWNT-polyamide thin film nanocomposite membranes showed higher property in hydrophilic surface and its water flux, as along with salt rejection, improved dramatically. The experimental results revealed that the modified SWNTs (especially those containing hydrophilic groups such as carboxyl groups and amino groups) well dispersed in the polyamide thin film layer, and hence improved the water permeation, and the salt rejection.

Secondary metabolites from marine sources have a wide range of biological activity. Marine natural products are promising candidates for lead pharmacological compounds to treat diseases that plague humans, including cancer. Cancer is a life-threatening disorder that has been difficult to overcome. It is a long-term illness that affects both young and old people. In recent years, significant attempts have been made to identify new anticancer drugs, as the existing drugs have been useless due to resistance of the malignant cells. Natural products derived from marine sources have been tested for their anticancer activity using a variety of cancer cell lines derived from humans and other sources, some of which have already been approved for clinical use, while some others are still being tested. These compounds can assault cancer cells via a variety of mechanisms, but certain cancer cells are resistant to them. As a result, the goal of this review was to look into the anticancer potential of marine natural products or their derivatives that were isolated from January 2019 to March 2020, in cancer cell lines, with a focus on the class and type of isolated compounds, source and location of isolation, cancer cell line type, and potency (IC50 values) of the isolated compounds that could be a guide for drug development.

Roof sliding and instability along the coal wall usually occur in the working face at large mining heights during the process of the first weighting, which causes roof cutting and support crushing. A mechanical model consists of the main roof, immediate roof, and support based on the nonlinear characteristics of the failure and instability of the immediate roof under the abutment pressure, which we constructed to study the step sinking of the main roof, as well as to assign the reasonable value of the support resistance during the first weighting. The instability mechanism of the system was studied by the catastrophe theory and the principle of energy conservation. A conclusion was drawn that the combined cantilever beam structure for the immediate roof will form with the increase of the mining height, and the instability of the immediate roof causes the catastrophic instability of the system. The system instability was found to be related to the stiffness ratio K, material parameters, the load Q, and the first weighting interval of the main roof by analyzing the necessary and sufficient conditions for system instability. The influence degree of each parameter on the stiffness ratio K was as follows: elastic modulus E > support stiffness k1 > cross-section area a > immediate roof thickness H. The calculation equations of support resistance and subsidence of roof step were obtained. The method of judging the roof instability using catastrophe theory was proved as reasonable on the basis of the monitoring example of no. 12401 working face in Shendong mining area, China. On this basis, a reasonable value of support resistance was further calculated, and the working face was maintained safely when the support resistance exceeded 19,232 kN.

Six new zinc(II) complexes were prepared by the reaction of ZnBr2 or ZnI2 with 4′-(substituted-phenyl)-2,2′:6′,2′′-terpyridine compounds, bearing p-methylsulfonyl (L1), p-methoxy (L2) and p-methyl (L3), which were characterized by elemental analysis, FT-IR, NMR and single crystal X-ray diffraction. The antiproliferative properties against Eca-109, A549 and Bel-7402 cell lines and the cytotoxicity test on RAW-264.7 of these compounds were monitored using a CCK-8 assay, and the studies indicate that the complexes show higher antiproliferative activities than cisplatin. The interactions of these complexes with CT-DNA and proteins (BSA) were studied by UV-Vis, circular dichroism (CD) and fluorescent spectroscopy, respectively. The results indicate that the interaction of these zinc(II) complexes with CT-DNA is achieved through intercalative binding, and their strong binding affinity to BSA is fulfilled through a static quenching mechanism. The simulation of the complexes with the CT-DNA fragment and BSA was studied by using molecular docking software. It further validates that the complexes interact with DNA through intercalative binding mode and that they have a strong interaction with BSA.

As the search for new antibiotics continues, the resistance to known antimicrobial compounds continues to increase. Many researchers around the world, in response to antibiotics resistance, have continued to search for new antimicrobial compounds in different ecological niches such as the marine environment. Marine habitats are one of the known and promising sources for bioactive compounds with antimicrobial potentials against currently drug-resistant strains of pathogenic microorganisms. For more than a decade, numerous antimicrobial compounds have been discovered from marine environments, with many more antimicrobials still being discovered every year. So far, only very few compounds are in preclinical and clinical trials. Research in marine natural products has resulted in the isolation and identification of numerous diverse and novel chemical compounds with potency against even drug-resistant pathogens. Some of these compounds, which mainly came from marine bacteria and fungi, have been classified into alkaloids, lactones, phenols, quinones, tannins, terpenes, glycosides, halogenated, polyketides, xanthones, macrocycles, peptides, and fatty acids. All these are geared towards discovering and isolating unique compounds with therapeutic potential, especially against multidrug-resistant pathogenic microorganisms. In this review, we tried to summarize published articles from 2015 to 2019 on antimicrobial compounds isolated from marine sources, including some of their chemical structures and tests performed against drug-resistant pathogens.

This paper first presents an adaptive expectation-maximization (AEM) control algorithm based on a measurement-data-driven model to reduce the variance of microelectromechanical system (MEMS) accelerometer sensor under multi disturbances. Significantly different characteristics of the disturbances, consisting of drastic-magnitude, short-duration vibration in the external environment, and slowly-varying, long-duration fluctuation inside the sensor are first constructed together with the measurement model of the accelerometer. Next, through establishing a data-driven model based on a historical small measurement sample, the window length of filter of the presented algorithm is adaptively chosen to estimate the sensor state and identify these disturbances simultaneously. Simulation results of the proposed AEM algorithm based on experimental test are compared with the Kalman filter (KF), least mean square (LMS), and regular EM (REM) methods. Variances of the estimated equivalent input under static condition are 0.212 mV, 0.149 mV, 0.015 mV, and 0.004 mV by the KF, LMS, REM, and AEM, respectively. Under dynamic conditions, the corresponding variances are 35.5 mV, 2.07 mV, 2.0 mV, and 1.45 mV, respectively. The variances under static condition based on the proposed method are reduced to 1.9%, 2.8%, and 27.3%, compared with the KF, LMS, and REM methods, respectively. The corresponding variances under dynamic condition are reduced to 4.1%, 70.1%, and 72.5%, respectively. The effectiveness of the proposed method is verified to reduce the variance of the MEMS resonant accelerometer sensor.

Reactions between a N6O4 macrocyclic ligand (L1) and several Zn(II) salts (trifluoromethane sulfonate, p-toluenesulfonate, acetate, benzoate, o-, m- or p-hydroxybenzoate) led to the formation of seven complexes, [Zn2L1 (DMSO)4](OSO2CF3)4 (1), [Zn2(p-OSO2PhCH3)4L1] (2), [Zn2(OCOCH3)4L1] (3), [Zn2(OCOPh)4L1] (4), [Zn2(o-OCOPhOH)4L1] (5), [Zn2(m-OCOPhOH)4 L1] (6) and [Zn2(p-OCOPhOH)4 L1] (7), which were characterized by elemental analysis, 1H-NMR, 13C-NMR, IR, fluorescence spectroscopies and single crystal X-ray diffraction. In 1, the Zn atom is pentacoordinated with a N3O2 irregular trigonal bipyramidal coordination environment, like the geometries in compounds 3–7, whereas in structure 2 the metal atom is envisaged as possessing a distorted N3O3 octahedronal environment. All the compounds show interesting photoluminescent properties in solid states and solutions in DMF and DMSO, which are reported along with their TG-DTA thermal decomposition processes, UV-vis absorption spectroscopy and fluorescence quantum yields in DMF and DMSO.