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6. Effect of antibiotic monensin on cell proliferation and IGF1R signaling pathway in human colorectal cancer cells.

Author

Zhou, Youping, Deng, Youlin, Wang, Jing, Yan, Zhengjian, Wei, Qiang, Ye, Jixing, Zhang, Junhui, He, Tong-Chuan, and Qiao, Min

Subjects
COLORECTAL cancer, MONENSIN, CANCER cells, CELL proliferation, CELLULAR signal transduction
Abstract

Colorectal cancer is the third leading cause of death in patients with cancers in America. Monensin has represented anti-cancer effect on various human cancer cells. We seek to investigate the effect of monensin on proliferation of human colorectal cancer cells and explore whether IGF1R signaling pathway is involved in anti-cancer mechanism of monensin. Cell proliferation and migration were assessed by crystal violet staining and cell wounding assay respectively. Cell apoptosis was analyzed by Hoechst 33258 staining and flow cytometry. Cell cycle progression was detected with the use of flow cytometry. Cancer-associated pathways were assessed with the use of pathway-specific reporters. Gene expression was detected by touchdown-quantitative real-time PCR. Inhibition of IGF1R was tested by immunofluorescence staining. Inhibition of IGF1R signaling was accomplished by adenovirus-mediated expression of IGF1. We found that monensin not only effectively inhibited cell proliferation, cell migration as well as cell cycle progression, but also induced apoptosis and G1 arrest in human colorectal cancer cells. Monensin was shown to target multiple cancer-related signaling pathways such as Elk1, AP1, as well as Myc/max, and suppressed IGF1R expression via increasing IGF1 in colorectal cancer cells. Monensin could suppressed IGF1R expression via increasing IGF1 in colorectal cancer cells. It has the potential to be repurposed as an anti-colorectal cancer agent, but further studies are still required to investigate the detailed mechanisms of monensin underlying its anti-cancer motion. Monensin inhibits the cell proliferation and the migration, induces apoptosis and inhibits cell cycle progression in human colorectal cancer cells. Monensin may exert anti-cancer activity by targeting multiple signaling pathways, including the IGF1R signaling pathway. Monensin has the potential to be repurposed as an anti-colorectal cancer agent. [ABSTRACT FROM AUTHOR]

Published
2023
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12. Correction: Adenovirus-Mediated Efficient Gene Transfer into Cultured Three-Dimensional Organoids

Author

Wang, Ning, primary, Zhang, Hongyu, additional, Zhang, Bing-Qiang, additional, Liu, Wei, additional, Zhang, Zhonglin, additional, Qiao, Min, additional, Zhang, Hongmei, additional, Deng, Fang, additional, Wu, Ningning, additional, Chen, Xian, additional, Wen, Sheng, additional, Zhang, Junhui, additional, Liao, Zhan, additional, Zhang, Qian, additional, Yan, Zhengjian, additional, Yin, Liangjun, additional, Ye, Jixing, additional, Deng, Youlin, additional, Luu, Hue H., additional, Haydon, Rex C., additional, Liang, Houjie, additional, and He, Tong-Chuan, additional

Published
2021
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14. A Blockade of IGF Signaling Sensitizes Human Ovarian Cancer Cells to the Anthelmintic Niclosamide-Induced Anti-Proliferative and Anticancer Activities

Author

Deng, Youlin, primary, Wang, Zhongliang, additional, Zhang, Fugui, additional, Qiao, Min, additional, Yan, Zhengjian, additional, Wei, Qiang, additional, Wang, Jing, additional, Liu, Hao, additional, Fan, Jiaming, additional, Zou, Yulong, additional, Liao, Junyi, additional, Hu, Xue, additional, Chen, Liqun, additional, Yu, Xinyi, additional, Haydon, Rex C., additional, Luu, Hue H., additional, Qi, Hongbo, additional, He, Tong-Chuan, additional, and Zhang, Junhui, additional

Published
2016
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16. Antibiotic monensin synergizes with EGFR inhibitors and oxaliplatin to suppress the proliferation of human ovarian cancer cells

Author

Deng, Youlin, primary, Zhang, Junhui, additional, Wang, Zhongliang, additional, Yan, Zhengjian, additional, Qiao, Min, additional, Ye, Jixing, additional, Wei, Qiang, additional, Wang, Jing, additional, Wang, Xin, additional, Zhao, Lianggong, additional, Lu, Shun, additional, Tang, Shengli, additional, Mohammed, Maryam K., additional, Liu, Hao, additional, Fan, Jiaming, additional, Zhang, Fugui, additional, Zou, Yulong, additional, Liao, Junyi, additional, Qi, Hongbo, additional, Haydon, Rex C., additional, Luu, Hue H., additional, He, Tong-Chuan, additional, and Tang, Liangdan, additional

Published
2015
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17. The Anthelmintic Drug Niclosamide Inhibits the Proliferative Activity of Human Osteosarcoma Cells by Targeting Multiple Signal Pathways

Author

Liao, Zhan, primary, Nan, Guoxin, additional, Yan, Zhengjian, additional, Zeng, Liyi, additional, Deng, Youlin, additional, Ye, Jixing, additional, Zhang, Zhonglin, additional, Qiao, Min, additional, Li, Ruifang, additional, Denduluri, Sahitya, additional, Wang, Jing, additional, Wei, Qiang, additional, Geng, Nisha, additional, Zhao, Lianggong, additional, Lu, Shun, additional, Wang, Xin, additional, Zhou, Guolin, additional, Luu, Hue, additional, Haydon, Rex, additional, He, Tong-Chuan, additional, and Wang, Zhongliang, additional

Published
2015
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18. TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR

Author

Zhang, Qian, primary, Wang, Jing, additional, Deng, Fang, additional, Yan, Zhengjian, additional, Xia, Yinglin, additional, Wang, Zhongliang, additional, Ye, Jixing, additional, Deng, Youlin, additional, Zhang, Zhonglin, additional, Qiao, Min, additional, Li, Ruifang, additional, Denduluri, Sahitya K., additional, Wei, Qiang, additional, Zhao, Lianggong, additional, Lu, Shun, additional, Wang, Xin, additional, Tang, Shengli, additional, Liu, Hao, additional, Luu, Hue H., additional, Haydon, Rex C., additional, He, Tong-Chuan, additional, and Jiang, Li, additional

Published
2015
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19. Reversibly Immortalized Mouse Articular Chondrocytes Acquire Long-Term Proliferative Capability While Retaining Chondrogenic Phenotype

Author

Lamplot, Joseph D., primary, Liu, Bo, additional, Yin, Liangjun, additional, Zhang, Wenwen, additional, Wang, Zhongliang, additional, Luther, Gaurav, additional, Wagner, Eric, additional, Li, Ruidong, additional, Nan, Guoxin, additional, Shui, Wei, additional, Yan, Zhengjian, additional, Rames, Richard, additional, Deng, Fang, additional, Zhang, Hongmei, additional, Liao, Zhan, additional, Liu, Wei, additional, Zhang, Junhui, additional, Zhang, Zhonglin, additional, Zhang, Qian, additional, Ye, Jixing, additional, Deng, Youlin, additional, Qiao, Min, additional, Haydon, Rex C., additional, Luu, Hue H., additional, Angeles, Jovito, additional, Shi, Lewis L., additional, He, Tong-Chuan, additional, and Ho, Sherwin H., additional

Published
2015
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20. Canonical Wnt signaling acts synergistically on BMP9-induced osteo/odontoblastic differentiation of stem cells of dental apical papilla (SCAPs)

Author

Zhang, Hongmei, primary, Wang, Jinhua, additional, Deng, Fang, additional, Huang, Enyi, additional, Yan, Zhengjian, additional, Wang, Zhongliang, additional, Deng, Youlin, additional, Zhang, Qian, additional, Zhang, Zhonglin, additional, Ye, Jixing, additional, Qiao, Min, additional, Li, Ruifang, additional, Wang, Jing, additional, Wei, Qiang, additional, Zhou, Guolin, additional, Luu, Hue H., additional, Haydon, Rex C., additional, He, Tong-Chuan, additional, and Deng, Feng, additional

Published
2015
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22. A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly

Author

Deng, Fang, primary, Chen, Xiang, additional, Liao, Zhan, additional, Yan, Zhengjian, additional, Wang, Zhongliang, additional, Deng, Youlin, additional, Zhang, Qian, additional, Zhang, Zhonglin, additional, Ye, Jixing, additional, Qiao, Min, additional, Li, Ruifang, additional, Denduluri, Sahitya, additional, Wang, Jing, additional, Wei, Qiang, additional, Li, Melissa, additional, Geng, Nisha, additional, Zhao, Lianggong, additional, Zhou, Guolin, additional, Zhang, Penghui, additional, Luu, Hue H., additional, Haydon, Rex C., additional, Reid, Russell R., additional, Yang, Tian, additional, and He, Tong-Chuan, additional

Published
2014
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24. Bone Morphogenetic Protein (BMP) signaling in development and human diseases

Author

Wang, Richard N., primary, Green, Jordan, additional, Wang, Zhongliang, additional, Deng, Youlin, additional, Qiao, Min, additional, Peabody, Michael, additional, Zhang, Qian, additional, Ye, Jixing, additional, Yan, Zhengjian, additional, Denduluri, Sahitya, additional, Idowu, Olumuyiwa, additional, Li, Melissa, additional, Shen, Christine, additional, Hu, Alan, additional, Haydon, Rex C., additional, Kang, Richard, additional, Mok, James, additional, Lee, Michael J., additional, Luu, Hue L., additional, and Shi, Lewis L., additional

Published
2014
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25. Bone morphogenetic protein 2 inhibits the proliferation and growth of human colorectal cancer cells

Author

ZHANG, YUNYUAN, primary, CHEN, XIAN, additional, QIAO, MIN, additional, ZHANG, BING-QIANG, additional, WANG, NING, additional, ZHANG, ZHONGLIN, additional, LIAO, ZHAN, additional, ZENG, LIYI, additional, DENG, YOULIN, additional, DENG, FANG, additional, ZHANG, JUNHUI, additional, YIN, LIANGJUN, additional, LIU, WEI, additional, ZHANG, QIAN, additional, YAN, ZHENGJIAN, additional, YE, JIXING, additional, WANG, ZHONGLIANG, additional, ZHOU, LAN, additional, LUU, HUE H., additional, HAYDON, REX C., additional, HE, TONG-CHUAN, additional, and ZHANG, HONGYU, additional

Published
2014
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26. Bone Morphogenetic Protein-9 Effectively Induces Osteo/Odontoblastic Differentiation of the Reversibly Immortalized Stem Cells of Dental Apical Papilla

Author

Wang, Jinhua, primary, Zhang, Hongmei, additional, Zhang, Wenwen, additional, Huang, Enyi, additional, Wang, Ning, additional, Wu, Ningning, additional, Wen, Sheng, additional, Chen, Xian, additional, Liao, Zhan, additional, Deng, Fang, additional, Yin, Liangjun, additional, Zhang, Junhui, additional, Zhang, Qian, additional, Yan, Zhengjian, additional, Liu, Wei, additional, Zhang, Zhonglin, additional, Ye, Jixing, additional, Deng, Youlin, additional, Luu, Hue H., additional, Haydon, Rex C., additional, He, Tong-Chuan, additional, and Deng, Feng, additional

Published
2014
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27. The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs)

Author

Wang, Ning, primary, Zhang, Wenwen, additional, Cui, Jing, additional, Zhang, Hongmei, additional, Chen, Xiang, additional, Li, Ruidong, additional, Wu, Ningning, additional, Chen, Xian, additional, Wen, Sheng, additional, Zhang, Junhui, additional, Yin, Liangjun, additional, Deng, Fang, additional, Liao, Zhan, additional, Zhang, Zhonglin, additional, Zhang, Qian, additional, Yan, Zhengjian, additional, Liu, Wei, additional, Ye, Jixing, additional, Deng, Youlin, additional, Wang, Zhongliang, additional, Qiao, Min, additional, Luu, Hue H., additional, Haydon, Rex C., additional, Shi, Lewis L., additional, Liang, Houjie, additional, and He, Tong-Chuan, additional

Published
2014
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28. Characterization of Constitutive Promoters for piggyBac Transposon-Mediated Stable Transgene Expression in Mesenchymal Stem Cells (MSCs)

Author

Wen, Sheng, primary, Zhang, Hongmei, additional, Li, Yasha, additional, Wang, Ning, additional, Zhang, Wenwen, additional, Yang, Ke, additional, Wu, Ningning, additional, Chen, Xian, additional, Deng, Fang, additional, Liao, Zhan, additional, Zhang, Junhui, additional, Zhang, Qian, additional, Yan, Zhengjian, additional, Liu, Wei, additional, Zhang, Zhonglin, additional, Ye, Jixing, additional, Deng, Youlin, additional, Zhou, Guolin, additional, Luu, Hue H., additional, Haydon, Rex C., additional, Shi, Lewis L., additional, He, Tong-Chuan, additional, and Wei, Guanghui, additional

Published
2014
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29. Adenovirus-Mediated Efficient Gene Transfer into Cultured Three-Dimensional Organoids

Author

Wang, Ning, primary, Zhang, Hongyu, additional, Zhang, Bing-Qiang, additional, Liu, Wei, additional, Zhang, Zhonglin, additional, Qiao, Min, additional, Zhang, Hongmei, additional, Deng, Fang, additional, Wu, Ningning, additional, Chen, Xian, additional, Wen, Sheng, additional, Zhang, Junhui, additional, Liao, Zhan, additional, Zhang, Qian, additional, Yan, Zhengjian, additional, Yin, Liangjun, additional, Ye, Jixing, additional, Deng, Youlin, additional, Luu, Hue H., additional, Haydon, Rex C., additional, Liang, Houjie, additional, and He, Tong-Chuan, additional

Published
2014
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30. Targeting BMP9-Promoted Human Osteosarcoma Growth by Inactivation of Notch Signaling

Author

Li, Ruidong, primary, Zhang, Wenwen, additional, Cui, Jing, additional, Shui, Wei, additional, Yin, Liangjun, additional, Wang, Yang, additional, Zhang, Hongyu, additional, Wang, Ning, additional, Wu, Ningning, additional, Nan, Guoxin, additional, Chen, Xian, additional, Wen, Sheng, additional, Deng, Fang, additional, Zhang, Hongmei, additional, Zhou, Guolin, additional, Liao, Zhan, additional, Zhang, Junhui, additional, Zhang, Qian, additional, Yan, Zhengjian, additional, Liu, Wei, additional, Zhang, Zhonglin, additional, Ye, Jixing, additional, Deng, Youlin, additional, Luu, Hue, additional, Haydon, Rex, additional, He, Tong-Chuan, additional, and Deng, Zhong-Liang, additional

Published
2014
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31. Adenovirus-Mediated Gene Transfer in Mesenchymal Stem Cells Can Be Significantly Enhanced by the Cationic Polymer Polybrene

Author

Zhao, Chen, primary, Wu, Ningning, additional, Deng, Fang, additional, Zhang, Hongmei, additional, Wang, Ning, additional, Zhang, Wenwen, additional, Chen, Xian, additional, Wen, Sheng, additional, Zhang, Junhui, additional, Yin, Liangjun, additional, Liao, Zhan, additional, Zhang, Zhonglin, additional, Zhang, Qian, additional, Yan, Zhengjian, additional, Liu, Wei, additional, Wu, Di, additional, Ye, Jixing, additional, Deng, Youlin, additional, Zhou, Guolin, additional, Luu, Hue H., additional, Haydon, Rex C., additional, Si, Weike, additional, and He, Tong-Chuan, additional

Published
2014
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32. Functional Characteristics of Reversibly Immortalized Hepatic Progenitor Cells Derived from Mouse Embryonic Liver

Author

Bi, Yang, primary, He, Yun, additional, Huang, Jiayi, additional, Su, Yuxi, additional, Zhu, Gao-Hui, additional, Wang, Yi, additional, Qiao, Min, additional, Zhang, Bing-Qiang, additional, Zhang, Hongyu, additional, Wang, Zhongliang, additional, Liu, Wei, additional, Cui, Jing, additional, Kang, Quan, additional, Zhang, Zhonglin, additional, Deng, Youlin, additional, Li, Ruifang, additional, Zhang, Qian, additional, Yang, Ke, additional, Luu, Hue H., additional, Haydon, Rex C., additional, He, Tong-Chuan, additional, and Tang, Ni, additional

Published
2014
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33. The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs).

Author

Wang, Ning, Zhang, Wenwen, Cui, Jing, Zhang, Hongmei, Chen, Xiang, Li, Ruidong, Wu, Ningning, Chen, Xian, Wen, Sheng, Zhang, Junhui, Yin, Liangjun, Deng, Fang, Liao, Zhan, Zhang, Zhonglin, Zhang, Qian, Yan, Zhengjian, Liu, Wei, Ye, Jixing, Deng, Youlin, and Wang, Zhongliang

Subjects
FIBROBLASTS, LABORATORY mice, EMBRYONIC stem cells, MESENCHYMAL stem cells, CELL differentiation, GENE expression, CELL proliferation
Abstract

Mouse embryonic fibroblasts (MEFs) are mesenchymal stem cell (MSC)-like multipotent progenitor cells and can undergo self-renewal and differentiate into to multiple lineages, including bone, cartilage and adipose. Primary MEFs have limited life span in culture, which thus hampers MEFs’ basic research and translational applications. To overcome this challenge, we investigate if piggyBac transposon-mediated expression of SV40 T antigen can effectively immortalize mouse MEFs and that the immortalized MEFs can maintain long-term cell proliferation without compromising their multipotency. Using the piggyBac vector MPH86 which expresses SV40 T antigen flanked with flippase (FLP) recognition target (FRT) sites, we demonstrate that mouse embryonic fibroblasts (MEFs) can be efficiently immortalized. The immortalized MEFs (piMEFs) exhibit an enhanced proliferative activity and maintain long-term cell proliferation, which can be reversed by FLP recombinase. The piMEFs express most MEF markers and retain multipotency as they can differentiate into osteogenic, chondrogenic and adipogenic lineages upon BMP9 stimulation in vitro. Stem cell implantation studies indicate that piMEFs can form bone, cartilage and adipose tissues upon BMP9 stimulation, whereas FLP-mediated removal of SV40 T antigen diminishes the ability of piMEFs to differentiate into these lineages, possibly due to the reduced expansion of progenitor populations. Our results demonstrate that piggyBac transposon-mediated expression of SV40 T can effectively immortalize MEFs and that the reversibly immortalized piMEFs not only maintain long-term cell proliferation but also retain their multipotency. Thus, the high transposition efficiency and the potential footprint-free natures may render piggyBac transposition an effective and safe strategy to immortalize progenitor cells isolated from limited tissue supplies, which is essential for basic and translational studies. [ABSTRACT FROM AUTHOR]

Published
2014
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34. Characterization of Constitutive Promoters for piggyBac Transposon-Mediated Stable Transgene Expression in Mesenchymal Stem Cells (MSCs).

Author

Wen, Sheng, Zhang, Hongmei, Li, Yasha, Wang, Ning, Zhang, Wenwen, Yang, Ke, Wu, Ningning, Chen, Xian, Deng, Fang, Liao, Zhan, Zhang, Junhui, Zhang, Qian, Yan, Zhengjian, Liu, Wei, Zhang, Zhonglin, Ye, Jixing, Deng, Youlin, Zhou, Guolin, Luu, Hue H., and Haydon, Rex C.

Subjects
TRANSPOSONS, TRANSGENE expression, MESENCHYMAL stem cells, COMPARATIVE studies, CYTOLOGY, DEVELOPMENTAL biology, PHYSIOLOGY
Abstract

Multipotent mesenchymal stem cells (MSCs) can undergo self-renewal and give rise to multi-lineages under given differentiation cues. It is frequently desirable to achieve a stable and high level of transgene expression in MSCs in order to elucidate possible molecular mechanisms through which MSC self-renewal and lineage commitment are regulated. Retroviral or lentiviral vector-mediated gene expression in MSCs usually decreases over time. Here, we choose to use the piggyBac transposon system and conduct a systematic comparison of six commonly-used constitutive promoters for their abilities to drive RFP or firefly luciferase expression in somatic HEK-293 cells and MSC iMEF cells. The analyzed promoters include three viral promoters (CMV, CMV-IVS, and SV40), one housekeeping gene promoter (UbC), and two composite promoters of viral and housekeeping gene promoters (hEFH and CAG-hEFH). CMV-derived promoters are shown to drive the highest transgene expression in HEK-293 cells, which is however significantly reduced in MSCs. Conversely, the composite promoter hEFH exhibits the highest transgene expression in MSCs whereas its promoter activity is modest in HEK-293 cells. The reduced transgene expression driven by CMV promoters in MSCs may be at least in part caused by DNA methylation, or to a lesser extent histone deacetlyation. However, the hEFH promoter is not significantly affected by these epigenetic modifications. Taken together, our results demonstrate that the hEFH composite promoter may be an ideal promoter to drive long-term and high level transgene expression using the piggyBac transposon vector in progenitor cells such as MSCs. [ABSTRACT FROM AUTHOR]

Published
2014
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35. [Analysis of two-dimension gel electrophoresis of human large cell lung cancer cell lines with different metastasis potentials].

Author

Zhu W, Deng Y, Zhou Q, Chen X, Wang Y, Liu L, and Che G

Abstract

Background: Metastasis is not only the malignant characteristics of lung can- cer, but also the chief cause of failure to cure and high mortality of lung cancer. To better explore and understand the mechanism of lung cancer metastasis and to search for potential markers for early diagnosing and reversing lung cancer metastasis, differential proteomic analysis is conducted in two human large cell lung cancer cell lines with high metastasis potentials (L9981) and low metastasis potentials (NL9980) by two-dimension gel electrophoresis (2-DE)., Methods: The total proteins of the two cell lines were separated by immobilized pH gradient (IPG)-based 2-DE. The differentially expressed proteins of the two cell lines were analyzed using image analysis software., Results: A high resolution and reproducible 2-DE image was successfully obtained. Average deviations for protein position in IEF direction were (0.858±0.076)mm and (1.514±0.127)mm in SDS-PAGE direction. The relative standard deviation for protein volume was (12.06±0.580)% in L9981 and (12.22±0.640)% in NL9980. The average total number of protein spots was 902±169 in L9981 cells and 941±173 in NL9980 cells in three repeated experiments. Image analysis of siliver-stained 2-DE image revealed that 4 protein spots had significant differential expressions in L9981 and NL9980 (student's t-test, P < 0.05). Fifteen protein spots were only detected in L9981, and 27 protein spots were only detected in NL9980., Conclusions: The results in this study suggest that an obviously differential proteomic expression exists between the human high- and low-metastatic large cell lung cancer cell lines. It will be helpful to further understand the molecular mechanisms of lung cancer invasion and metastasis, and provide new experimental evidence for searching metastatic-related molecule of lung cancer.

Published
2005
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