Search

Your search keyword '"Deng, Qiuting"' showing total 31 results
Start Over Author "Deng, Qiuting"
31 results on '"Deng, Qiuting"'

2. A spatiotemporal atlas of cholestatic injury and repair in mice

Author

Wu, Baihua, Shentu, Xinyi, Nan, Haitao, Guo, Pengcheng, Hao, Shijie, Xu, Jiangshan, Shangguan, Shuncheng, Cui, Lei, Cen, Jin, Deng, Qiuting, Wu, Yan, Liu, Chang, Song, Yumo, Lin, Xiumei, Wang, Zhifeng, Yuan, Yue, Ma, Wen, Li, Ronghai, Li, Yikang, Qian, Qiwei, Du, Wensi, Lai, Tingting, Yang, Tao, Liu, Chuanyu, Ma, Xiong, Chen, Ao, Xu, Xun, Lai, Yiwei, Liu, Longqi, Esteban, Miguel A., and Hui, Lijian

Published
2024
Full Text
View/download PDF

3. A spatiotemporal atlas of mouse liver homeostasis and regeneration

Author

Xu, Jiangshan, Guo, Pengcheng, Hao, Shijie, Shangguan, Shuncheng, Shi, Quan, Volpe, Giacomo, Huang, Keke, Zuo, Jing, An, Juan, Yuan, Yue, Cheng, Mengnan, Deng, Qiuting, Zhang, Xiao, Lai, Guangyao, Nan, Haitao, Wu, Baihua, Shentu, Xinyi, Wu, Liang, Wei, Xiaoyu, Jiang, Yujia, Huang, Xin, Pan, Fengyu, Song, Yumo, Li, Ronghai, Wang, Zhifeng, Liu, Chuanyu, Liu, Shiping, Li, Yuxiang, Yang, Tao, Xu, Zhicheng, Du, Wensi, Li, Ling, Ahmed, Tanveer, You, Kai, Dai, Zhen, Li, Li, Qin, Baoming, Li, Yinxiong, Lai, Liangxue, Qin, Dajiang, Chen, Junling, Fan, Rong, Li, Yongyin, Hou, Jinlin, Ott, Michael, Sharma, Amar Deep, Cantz, Tobias, Schambach, Axel, Kristiansen, Karsten, Hutchins, Andrew P., Göttgens, Berthold, Maxwell, Patrick H., Hui, Lijian, Xu, Xun, Liu, Longqi, Chen, Ao, Lai, Yiwei, and Esteban, Miguel A.

Published
2024
Full Text
View/download PDF

7. Rolling back human pluripotent stem cells to an eight-cell embryo-like stage

Author

Mazid, Md. Abdul, Ward, Carl, Luo, Zhiwei, Liu, Chuanyu, Li, Yunpan, Lai, Yiwei, Wu, Liang, Li, Jinxiu, Jia, Wenqi, Jiang, Yu, Liu, Hao, Fu, Lixin, Yang, Yueli, Ibañez, David P., Lai, Junjian, Wei, Xiaoyu, An, Juan, Guo, Pengcheng, Yuan, Yue, Deng, Qiuting, Wang, Yang, Liu, Ying, Gao, Fei, Wang, Junwen, Zaman, Shahriar, Qin, Baoming, Wu, Guangming, Maxwell, Patrick H., Xu, Xun, Liu, Longqi, Li, Wenjuan, and Esteban, Miguel A.

Published
2022
Full Text
View/download PDF

8. Cell transcriptomic atlas of the non-human primate Macaca fascicularis

Author

Han, Lei, Wei, Xiaoyu, Liu, Chuanyu, Volpe, Giacomo, Zhuang, Zhenkun, Zou, Xuanxuan, Wang, Zhifeng, Pan, Taotao, Yuan, Yue, Zhang, Xiao, Fan, Peng, Guo, Pengcheng, Lai, Yiwei, Lei, Ying, Liu, Xingyuan, Yu, Feng, Shangguan, Shuncheng, Lai, Guangyao, Deng, Qiuting, Liu, Ya, Wu, Liang, Shi, Quan, Yu, Hao, Huang, Yunting, Cheng, Mengnan, Xu, Jiangshan, Liu, Yang, Wang, Mingyue, Wang, Chunqing, Zhang, Yuanhang, Xie, Duo, Yang, Yunzhi, Yu, Yeya, Zheng, Huiwen, Wei, Yanrong, Huang, Fubaoqian, Lei, Junjie, Huang, Waidong, Zhu, Zhiyong, Lu, Haorong, Wang, Bo, Wei, Xiaofeng, Chen, Fengzhen, Yang, Tao, Du, Wensi, Chen, Jing, Xu, Shibo, An, Juan, Ward, Carl, Wang, Zongren, Pei, Zhong, Wong, Chi-Wai, Liu, Xiaolei, Zhang, Huafeng, Liu, Mingyuan, Qin, Baoming, Schambach, Axel, Isern, Joan, Feng, Liqiang, Liu, Yan, Guo, Xiangyu, Liu, Zhen, Sun, Qiang, Maxwell, Patrick H., Barker, Nick, Muñoz-Cánoves, Pura, Gu, Ying, Mulder, Jan, Uhlen, Mathias, Tan, Tao, Liu, Shiping, Yang, Huanming, Wang, Jian, Hou, Yong, Xu, Xun, Esteban, Miguel A., and Liu, Longqi

Published
2022
Full Text
View/download PDF

11. Single‐cell multi‐modal chromatin profiles revealing epigenetic regulations of cells in hepatocellular carcinoma.

Author

Wang, Chunqing, Huang, Waidong, Zhong, Yu, Zou, Xuanxuan, Liu, Shang, Li, Jie, Sun, Yunfan, Zhou, Kaiqian, Chen, Xi, Li, Zihao, Wang, Shanshan, Huang, Yaling, Bai, Yinqi, Yin, Jianhua, Jin, Xin, Liu, Shiping, Yuan, Yue, Deng, Qiuting, Jiang, Miaomiao, and Liu, Chuanyu

Subjects
GENETIC regulation, GENE expression, DNA fingerprinting, CELLULAR control mechanisms, EPIGENOMICS, CHROMATIN
Abstract

Background: Various epigenetic regulations systematically govern gene expression in cells involving various biological processes. Dysregulation of the epigenome leads to aberrant transcriptional programs and subsequently results in diseases, such as cancer. Therefore, comprehensive profiling epigenomics is essential for exploring the mechanisms underlying gene expression regulation during development and disease. Methods: In this study, we developed single‐cell chromatin proteins and accessibility tagmentation (scCPA‐Tag), a multi‐modal single‐cell epigenetic profile capturing technique based on barcoded Tn5 transposases and a droplet microfluidics platform. scCPA‐Tag enables the simultaneous capture of DNA profiles of histone modification and chromatin accessibility in the same cell. Results: By applying scCPA‐Tag to K562 cells and a hepatocellular carcinoma (HCC) sample, we found that the silence of several chromatin‐accessible genes can be attributed to lysine‐27‐trimethylation of the histone H3 tail (H3K27me3) modification. We characterized the epigenetic features of the tumour cells and different immune cell types in the HCC tumour tissue by scCPA‐Tag. Besides, a tumour cell subtype (C2) with more aggressive features was identified and characterized by high chromatin accessibility and a lower abundance of H3K27me3 on tumour‐promoting genes. Conclusions: Our multi‐modal scCPA‐Tag provides a comprehensive approach for exploring the epigenetic landscapes of heterogeneous cell types and revealing the mechanisms of gene expression regulation during developmental and pathological processes at the single‐cell level. Highlights: scCPA‐Tag offers a highly efficient and high throughput technique to simultaneously profile histone modification and chromatin accessibility within a single cell.scCPA‐Tag enables to uncover multiple epigenetic modification features of cellular compositions within tumor tissues.scCPA‐Tag facilitates the exploration of the epigenetic landscapes of heterogeneous cell types and provides the mechanisms governing gene expression regulation. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

12. Single-cell chromatin accessibility profiling of cell-state-specific gene regulatory programs during mouse organogenesis

Author

Deng, Qiuting, primary, Wang, Shengpeng, additional, Huang, Zijie, additional, Lan, Qing, additional, Lai, Guangyao, additional, Xu, Jiangshan, additional, Yuan, Yue, additional, Liu, Chang, additional, Lin, Xiumei, additional, Feng, Weimin, additional, Ma, Wen, additional, Cheng, Mengnan, additional, Hao, Shijie, additional, Duan, Shanshan, additional, Zheng, Huiwen, additional, Chen, Xiaoyan, additional, Hou, Yong, additional, Luo, Yingjie, additional, Liu, Longqi, additional, and Liu, Chuanyu, additional

Published
2023
Full Text
View/download PDF

14. Single-cell transcriptional diversity of neonatal umbilical cord blood immune cells reveals neonatal immune tolerance

Author

Shi, Xuyang, primary, Ma, Wen, additional, Duan, Shanshan, additional, Shi, Quan, additional, Wu, Shuo, additional, Hao, Shijie, additional, Dong, Guoyi, additional, Li, Jinxiu, additional, Song, Yumo, additional, Liu, Chang, additional, Lin, Xiumei, additional, Yuan, Yue, additional, Deng, Qiuting, additional, Xu, Jiangshan, additional, Bai, Shiyi, additional, Hou, Yong, additional, Liu, Chuanyu, additional, and Liu, Longqi, additional

Published
2022
Full Text
View/download PDF

15. A Cellular Resolution Spatial Transcriptomic Landscape of the Medial Structures in Postnatal Mouse Brain

Author

Cheng, Mengnan, primary, Wu, Liang, additional, Han, Lei, additional, Huang, Xin, additional, Lai, Yiwei, additional, Xu, Jiangshan, additional, Wang, Shuai, additional, Li, Mei, additional, Zheng, Huiwen, additional, Feng, Weimin, additional, Huang, Zirui, additional, Jiang, Yujia, additional, Hao, Shijie, additional, Li, Zhao, additional, Chen, Xi, additional, Peng, Jian, additional, Guo, Pengcheng, additional, Zhang, Xiao, additional, Lai, Guangyao, additional, Deng, Qiuting, additional, Yuan, Yue, additional, Yang, Fangming, additional, Wei, Xiaoyu, additional, Liao, Sha, additional, Chen, Ao, additional, Volpe, Giacomo, additional, Esteban, Miguel A., additional, Hou, Yong, additional, Liu, Chuanyu, additional, and Liu, Longqi, additional

Published
2022
Full Text
View/download PDF

16. Transcriptomic Profile of the Mouse Postnatal Liver Development by Single-Nucleus RNA Sequencing

Author

Xu, Jiangshan, Hao, Shijie, Shi, Quan, Deng, Qiuting, Jiang, Yujia, Guo, Pengcheng, Yuan, Yue, Shi, Xuyang, Shangguan, Shuncheng, Zheng, Huiwen, Lai, Guangyao, Huang, Yaling, Wang, Yang, Song, Yumo, Liu, Yang, Wu, Liang, Wang, Zhifeng, Cheng, Jiehui, Wei, Xiaoyu, Cheng, Mengnan, Lai, Yiwei, Volpe, Giacomo, Esteban, Miguel A., Hou, Yong, Liu, Chuanyu, Liu, Longqi, Xu, Jiangshan, Hao, Shijie, Shi, Quan, Deng, Qiuting, Jiang, Yujia, Guo, Pengcheng, Yuan, Yue, Shi, Xuyang, Shangguan, Shuncheng, Zheng, Huiwen, Lai, Guangyao, Huang, Yaling, Wang, Yang, Song, Yumo, Liu, Yang, Wu, Liang, Wang, Zhifeng, Cheng, Jiehui, Wei, Xiaoyu, Cheng, Mengnan, Lai, Yiwei, Volpe, Giacomo, Esteban, Miguel A., Hou, Yong, Liu, Chuanyu, and Liu, Longqi

Published
2022

17. Transcriptomic Profile of the Mouse Postnatal Liver Development by Single-Nucleus RNA Sequencing

Author

Xu, Jiangshan, primary, Hao, Shijie, additional, Shi, Quan, additional, Deng, Qiuting, additional, Jiang, Yujia, additional, Guo, Pengcheng, additional, Yuan, Yue, additional, Shi, Xuyang, additional, Shangguan, Shuncheng, additional, Zheng, Huiwen, additional, Lai, Guangyao, additional, Huang, Yaling, additional, Wang, Yang, additional, Song, Yumo, additional, Liu, Yang, additional, Wu, Liang, additional, Wang, Zhifeng, additional, Cheng, Jiehui, additional, Wei, Xiaoyu, additional, Cheng, Mengnan, additional, Lai, Yiwei, additional, Volpe, Giacomo, additional, Esteban, Miguel A., additional, Hou, Yong, additional, Liu, Chuanyu, additional, and Liu, Longqi, additional

Published
2022
Full Text
View/download PDF

18. Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Mesenchymal Stem/Stromal Cells Derived from Human Placenta

Author

Li, Jinlu, primary, Wang, Quanlei, additional, An, Yanru, additional, Chen, Xiaoyan, additional, Xing, Yanan, additional, Deng, Qiuting, additional, Li, Zelong, additional, Wang, Shengpeng, additional, Dai, Xi, additional, Liang, Ning, additional, Hou, Yong, additional, Yang, Huanming, additional, and Shang, Zhouchun, additional

Published
2022
Full Text
View/download PDF

19. Single-cell transcriptional profiling reveals cellular and molecular divergence in human maternal-fetal interface

Author

Wang, Quanlei, primary, Li, Jinlu, additional, Wang, Shengpeng, additional, Deng, Qiuting, additional, An, Yanru, additional, Xing, Yanan, additional, Dai, Xi, additional, Li, Zelong, additional, Ma, Qiwang, additional, Wang, Kuixing, additional, Liu, Chuanyu, additional, Yuan, Yue, additional, Dong, Guoyi, additional, Zhang, Tao, additional, Yang, Huanming, additional, Du, Yutao, additional, Hou, Yong, additional, Ke, Weilin, additional, and Shang, Zhouchun, additional

Published
2022
Full Text
View/download PDF

20. Comparative Analysis Of Mesenchymal Stem/Stromal Cells Derived from Human Induced Pluripotent Stem Cells and the Cognate Umbilical Cord Mesenchymal Stem/Stromal Cells

Author

Wang, Quanlei, primary, Wang, Yuwei, additional, Chang, Chongfei, additional, Ma, Feilong, additional, Peng, Dongxiu, additional, Yang, Shun, additional, An, Yanru, additional, Deng, Qiuting, additional, Wang, Qixiao, additional, Gao, Fei, additional, Wang, Fei, additional, Tang, Huiru, additional, Qi, Xu-Feng, additional, Jiang, Xiaoming, additional, Cai, Dongqing, additional, and Zhou, Guangqian, additional

Published
2022
Full Text
View/download PDF

21. Cell transcriptomic atlas of the non-human primate Macaca fascicularis

Author

Han, Lei, primary, Wei, Xiaoyu, additional, Liu, Chuanyu, additional, Volpe, Giacomo, additional, Zhuang, Zhenkun, additional, Zou, Xuanxuan, additional, Wang, Zhifeng, additional, Pan, Taotao, additional, Yuan, Yue, additional, Zhang, Xiao, additional, Fan, Peng, additional, Guo, Pengcheng, additional, Lai, Yiwei, additional, Lei, Ying, additional, Liu, Xingyuan, additional, Yu, Feng, additional, Shangguan, Shuncheng, additional, Lai, Guangyao, additional, Deng, Qiuting, additional, Liu, Ya, additional, Wu, Liang, additional, Shi, Quan, additional, Yu, Hao, additional, Huang, Yunting, additional, Cheng, Mengnan, additional, Xu, Jiangshan, additional, Liu, Yang, additional, Wang, Mingyue, additional, Wang, Chunqing, additional, Zhang, Yuanhang, additional, Xie, Duo, additional, Yang, Yunzhi, additional, Yu, Yeya, additional, Zheng, Huiwen, additional, Wei, Yangrong, additional, Huang, Fubaoqian, additional, Lei, Junjie, additional, Huang, Waidong, additional, Zhu, Zhiyong, additional, Lu, Haorong, additional, Wang, Bo, additional, Wei, Xiaofeng, additional, Chen, Fengzhen, additional, Yang, Tao, additional, Du, Wensi, additional, Chen, Jing, additional, Xu, Shibo, additional, An, Juan, additional, Ward, Carl, additional, Wang, Zongren, additional, Pei, Zhong, additional, Wong, Chi-Wai, additional, Liu, Xiaolei, additional, Zhang, Huafeng, additional, Liu, Mingyuan, additional, Qin, Baoming, additional, Schambach, Axel, additional, Isern, Joan, additional, Feng, Liqiang, additional, Liu, Yan, additional, Guo, Xiangyu, additional, Liu, Zhen, additional, Sun, Qiang, additional, Maxwell, Patrick H., additional, Barker, Nick, additional, Munoz-Canoves, Pura, additional, Gu, Ying, additional, Mulder, Jan, additional, Uhlen, Mathias, additional, Tan, Tao, additional, Liu, Shiping, additional, Yang, Huanming, additional, Wang, Jian, additional, Hou, Yong, additional, Xu, Xun, additional, Esteban, Miguel A., additional, and Liu, Longqi, additional

Published
2021
Full Text
View/download PDF

22. The Chromatin Accessibility Landscape of Adult Rat

Author

Yuan, Yue, primary, Deng, Qiuting, additional, Wei, Xiaoyu, additional, Liu, Yang, additional, Lan, Qing, additional, Jiang, Yu, additional, Yu, Yeya, additional, Guo, Pengcheng, additional, Xu, Jiangshan, additional, Yu, Cong, additional, Han, Lei, additional, Cheng, Mengnan, additional, Wu, Peiying, additional, Zhang, Xiao, additional, Lai, Yiwei, additional, Volpe, Giacomo, additional, Esteban, Miguel A., additional, Yang, Huanming, additional, Liu, Chuanyu, additional, and Liu, Longqi, additional

Published
2021
Full Text
View/download PDF

23. Single-Nucleus Chromatin Accessibility Landscape Reveals Diversity in Regulatory Regions Across Distinct Adult Rat Cortex

Author

Yu, Yeya, primary, Wei, Xiaoyu, additional, Deng, Qiuting, additional, Lan, Qing, additional, Guo, Yiping, additional, Han, Lei, additional, Yuan, Yue, additional, Fan, Peng, additional, Wu, Peiying, additional, Shangguan, Shuncheng, additional, Liu, Yang, additional, Lai, Yiwei, additional, Volpe, Giacomo, additional, Esteban, Miguel A., additional, Liu, Chuanyu, additional, Hou, Yong, additional, and Liu, Longqi, additional

Published
2021
Full Text
View/download PDF

24. Single‐cell transcriptome profiling reveals molecular heterogeneity in human umbilical cord tissue and culture‐expanded mesenchymal stem cells

Author

Wang, Quanlei, primary, Li, Jinlu, additional, Wang, Shengpeng, additional, Deng, Qiuting, additional, Wang, Kuixing, additional, Dai, Xi, additional, An, Yanru, additional, Dong, Guoyi, additional, Ke, Weilin, additional, Chen, Fang, additional, Liu, Longqi, additional, Yang, Huanming, additional, Du, Yutao, additional, Zhao, Weihua, additional, and Shang, Zhouchun, additional

Published
2021
Full Text
View/download PDF

25. An ATAC-seq Dataset Uncovers the Regulatory Landscape During Axolotl Limb Regeneration

Author

Wei, Xiaoyu, primary, Li, Hanbo, additional, Guo, Yang, additional, Zhao, Xiaoying, additional, Liu, Yang, additional, Zou, Xuanxuan, additional, Zhou, Li, additional, Yuan, Yue, additional, Qin, Yating, additional, Mao, Chunyan, additional, Huang, Guodong, additional, Yu, Yeya, additional, Deng, Qiuting, additional, Feng, Weimin, additional, Xu, Jiangshan, additional, Wang, Mingyue, additional, Liu, Shanshan, additional, Yang, Huanming, additional, Liu, Longqi, additional, Liu, Chuanyu, additional, and Gu, Ying, additional

Published
2021
Full Text
View/download PDF

26. Molecular Identification of Commercial Fish Maws by DNA Sequencing of 16S rRNA and Cytochrome cOxidase I Genes

Author

Zhang, Jinju, Deng, Qiuting, Liu, Xindan, Wu, Menghua, Ma, Zhiguo, Shaw, Pang-Chui, Zhang, Ying, and Cao, Hui

Abstract

Fish maws (dried swim bladders) have long been used for medicinal tonics and as a valuable food resource in Southeast Asia. However, it is difficult to identify the original species of fish maws sold in markets due to a lack of taxonomic characteristics. In the present study, 37 kinds of commercial fish maws from various medicinal material markets were examined, and gene sequences were successfully obtained from ca. 95% of the samples. Partial sequences of the 16S rRNA gene and cytochrome coxidase I (COI) gene were obtained and used to investigate the origin of these commercial fish maws. Thirty-five specimens belonged to nine species: five croakers and four noncroakers. All species identification was supported by both high homogeneity (98 to 100%) and clear clustering with low within-group Kimura two-parameter divergence scores (0 to 0.04 for 16S rRNA and 0 to 0.07 for COI) and high between-group divergence scores (0.07 to 0.15 for 16S rRNA and 0.11 to 0.24 for COI). Croakers were the predominant species, accounting for 74% of the total fish maw specimens. The large demand for croakers has put some species at the risk of extinction due to overfishing. As a valuable food, fish maw has progressively become more popular and has been used as a substitute for shark fin. The identification results allowed us to learn more about the fish species available on the fish maw market and provided an indicator for possible control of threatened or endangered fish species. A probable correlation between the molecular characteristics and morphological features of fish maws was also found and could provide both consumers and merchants with an important reference for identifying the origin of fish maws.

Published
2022
Full Text
View/download PDF

27. Single-Cell Sequencing of Peripheral Mononuclear Cells Reveals Distinct Immune Response Landscapes of COVID-19 and Influenza Patients

Author

Zhu, Linnan, primary, Yang, Penghui, additional, Zhao, Yingze, additional, Zhuang, Zhenkun, additional, Wang, Zhifeng, additional, Song, Rui, additional, Zhang, Jie, additional, Liu, Chuanyu, additional, Gao, Qianqian, additional, Xu, Qumiao, additional, Wei, Xiaoyu, additional, Sun, Hai-Xi, additional, Ye, Beiwei, additional, Wu, Yanan, additional, Zhang, Ning, additional, Lei, Guanglin, additional, Yu, Lingxiang, additional, Yan, Jin, additional, Diao, Guanghao, additional, Meng, Fanping, additional, Bai, Changqing, additional, Mao, Panyong, additional, Yu, Yeya, additional, Wang, Mingyue, additional, Yuan, Yue, additional, Deng, Qiuting, additional, Li, Ziyi, additional, Huang, Yunting, additional, Hu, Guohai, additional, Liu, Yang, additional, Wang, Xiaoqian, additional, Xu, Ziqian, additional, Liu, Peipei, additional, Bi, Yuhai, additional, Shi, Yi, additional, Zhang, Shaogeng, additional, Chen, Zhihai, additional, Wang, Jian, additional, Xu, Xun, additional, Wu, Guizhen, additional, Wang, Fu-Sheng, additional, Gao, George F., additional, Liu, Longqi, additional, and Liu, William J., additional

Published
2020
Full Text
View/download PDF

30. Single-cell RNA-seq reveals dynamic transcriptome profiling in human early neural differentiation

Author

Shang, Zhouchun, Chen, Dongsheng, Wang, Quanlei, Wang, Shengpeng, Deng, Qiuting, Wu, Liang, Liu, Chuanyu, Ding, Xiangning, Wang, Shiyou, Zhong, Jixing, Zhang, Doudou, Cai, Xiaodong, Zhu, Shida, Yang, Huanming, Liu, Longqi, Fink, J Lynn, Chen, Fang, Liu, Xiaoqing, Gao, Zhengliang, Xu, Xun, Shang, Zhouchun, Chen, Dongsheng, Wang, Quanlei, Wang, Shengpeng, Deng, Qiuting, Wu, Liang, Liu, Chuanyu, Ding, Xiangning, Wang, Shiyou, Zhong, Jixing, Zhang, Doudou, Cai, Xiaodong, Zhu, Shida, Yang, Huanming, Liu, Longqi, Fink, J Lynn, Chen, Fang, Liu, Xiaoqing, Gao, Zhengliang, and Xu, Xun

Abstract

Background: Investigating cell fate decision and subpopulation specification in the context of the neural lineage is fundamental to understanding neurogenesis and neurodegenerative diseases. The differentiation process of neural-tube-like rosettes in vitro is representative of neural tube structures, which are composed of radially organized, columnar epithelial cells and give rise to functional neural cells. However, the underlying regulatory network of cell fate commitment during early neural differentiation remains elusive. Results: In this study, we investigated the genome-wide transcriptome profile of single cells from six consecutive reprogramming and neural differentiation time points and identified cellular subpopulations present at each differentiation stage. Based on the inferred reconstructed trajectory and the characteristics of subpopulations contributing the most toward commitment to the central nervous system lineage at each stage during differentiation, we identified putative novel transcription factors in regulating neural differentiation. In addition, we dissected the dynamics of chromatin accessibility at the neural differentiation stages and revealed active cis-regulatory elements for transcription factors known to have a key role in neural differentiation as well as for those that we suggest are also involved. Further, communication network analysis demonstrated that cellular interactions most frequently occurred in the embryoid body stage and that each cell subpopulation possessed a distinctive spectrum of ligands and receptors associated with neural differentiation that could reflect the identity of each subpopulation. Conclusions: Our study provides a comprehensive and integrative study of the transcriptomics and epigenetics of human early neural differentiation, which paves the way for a deeper understanding of the regulatory mechanisms driving the differentiation of the neural lineage.

Published
2018

31. Single-cell RNA-seq reveals dynamic transcriptome profiling in human early neural differentiation

Author

Shang, Zhouchun, primary, Chen, Dongsheng, additional, Wang, Quanlei, additional, Wang, Shengpeng, additional, Deng, Qiuting, additional, Wu, Liang, additional, Liu, Chuanyu, additional, Ding, Xiangning, additional, Wang, Shiyou, additional, Zhong, Jixing, additional, Zhang, Doudou, additional, Cai, Xiaodong, additional, Zhu, Shida, additional, Yang, Huanming, additional, Liu, Longqi, additional, Fink, J Lynn, additional, Chen, Fang, additional, Liu, Xiaoqing, additional, Gao, Zhengliang, additional, and Xu, Xun, additional

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results