Search

Additional file 1: Figure S1. The schematic diagram of biofilters setup. Figure S2. Schematic diagram of biofilm adhesion strength test. Figure S3. Effect of different dosage of Rhodococcus sp.BH4 on biofilm formation. Figure S4. (a) AHL quenching rate and (b) quorum quenching activity towards the exogenous AHL in the biofilm samples on Days 25, 45, 65 and 90. Figure S5. The sample distance matrix obtained based on the bray Curtis algorithm. The results were used to test whether the difference between groups is significantly greater than the difference within the group. Figure S6. Chao1 index, number of OTUs, Shannon index and pielou_e index in BF and QQBF biofilms. Figure S7. Relative abundance of biomarkers at the phylum level in (a) BF and (b) QQBF. The biomarkers were chosen through Kruskal-Wallis test. Figure S8. Comparison of relative abundance of QS-related bacteria in BF and QQBF biofilms at day 25 and 90. Figure S9. Comparison of gene expression levels in microbial samples at day 90 between BF and QQBF based on metagenomic analysis. Figure S10. Comparison of the number of up-regulated and down-regulated genes in microbial samples (BF vs QQBF) at day 90 of BF and QQBF based on metagenomic analysis. Figure S11. Differential gene enrichment results in microbial samples at day 90 of BF and QQBF based on KEGG database annotations. Figure S12. Up-regulation of QS genes in BF biofilm samples compared to QQBF in QS regulatory pathways based on metagenomic analysis. Note: The fold difference in relative abundance of genes in BF and QQBF were treated using Exponential. Figure S13. Test results of the degradation of toluene by Rhodococcus sp. BH4. Table S1. Development of waste gas biofilter technology. Table S2. Composition of the nutrient solution. Table S3. The operating conditions of the two biofilters. Table S4. The results of Ergun equation fitting at the 25th day and the 65th day. Table S5. Statistic differences in EPS concentrations. Table S6. Quenching rate (k) of AHLs in sludge samples at different times. Table S7. The description and relative abundance of QS-related genes in BF and QQBF samples. Table S8. Topological properties of the empirical pMENs of the biofilm communities in BF and QQBF biofilms during the operation and their associated random pMENs. Table S9. Keystone species' centrality indexes of networks in biofilm communities sourced from BF and QQBF biofilms. Table S10. Taxonomic information and average abundance of keystone taxa observed in ecological networks in biofilm communities sourced from BF and QQBF biofilms. Table S11. Typical QS genes description and function in QS regulatory pathways in BF and QQBF biofilm samples based on metagenomic analysis. Table S12. Summary of the role of Rhodococcus sp. BH4 in bioreactors. Supplementary Method S1. The biofilm adhesion strength test. Supplementary Method S2. 16S rDNA sequencing. Supplementary Method S3. Metagenomics Sequencing Analysis. Supplementary Method S4. The calculation of biofilter performance and pressure drop curve fitting methods.

To analyze the relevant data, including medical history, treatment and nursing of one cysticercosis patient with multiple foci in Baise City, so as to provide the evidence for improving the treatment and nursing of cysticercosis in the future.The data of diet history, diagnosis, treatment and nursing of the patient were collected and analyzed.The patient was a permanent resident in a cysticercosis high endemic area and had a definite history of eating measly pork. The combination of clinical manifestations and auxiliary examinations of the patient confirmed that he was suffered from cysticercosis with multiple foci. After the patient was firstly administered the antiparasitic treatment of cysticercosis (praziquantel), he had status epilepticus. However, with the close cooperation between physicians and nurses, his illness obviously improved and 35 days later, he discharged.The physicians and nurses should pay much attention to the changes of illness of the patients with cysticercosis, especially the cysticercosis patients with multiple foci in the antiparasitic treatment of cysticercosis, so as to avoid cerebral herniation due to the increased intracranial pressure.[

To evaluate the monitoring results of medium- and long-term program of schistosomiasis control in Baise City, so as to provide the reference for the elimination of schistosomiasis.The data of schistosomiasis control in Baise City from 2004 to 2015 were collected and analyzed.By the end of 2015, the total number of regular screening serum tests for schistosomiasis in the planning period was 10 244 person-times, with 649 positive cases. The number of feces tests was 2 158 person-times in the permanent resident population, and the number was 2 683 person-times in the floating population. TheThe effect of medium- and long-term program of schistosomiasis control is effective in Baise City, and the criterion of schistosomiasis elimination has been achieved.

To analyze the assessment results and summary the work experience of malaria elimination in Baise City.According toSince 2009, there were no local malaria cases and imported secondary cases in Baise City for the 6 consecutive years, and the detailed data were collected and the self-assessment reports of malaria elimination were written in all the counties. There was no omission or delay of malaria case reports in the 12 counties. The highest score of the examination and evaluation was 96.58 points, the lowest was 90.76 points, and the average was 93.77 points. The biggest impact on the evaluation scores was "on-site examination".All the 12 counties (county-level city or district) of Baise City have passed the municipal examination and evaluation of malaria elimination, and the key of next work is malaria monitoring, timely finding and treating the imported malaria patients, and to guarantee no imported secondary cases.

To understand the schistosomiasis epidemic and control history in Jingxi City, so as to provide the evidence for improving the future work.The data of schistosomiasis prevention and control work were collected and analyzed comprehensively in Jingxi City from 1956-2015.From 1956, the schistosomiasis prevention and control work carried out, and in 1985, Jingxi City reached the standard of schistosomiasis transmission interrupted and no local schistosome infected residents and livestock were found for 36 years. In May 2016, the city reached the national standard of Schistosomiasis Elimination Assessment Review. The cumulative number of schistosomiasis detection of residents was 348 801 person-times, and 10 434 schistosomiasis patients were found. The cumulative number of schistosomiasis detection of cattle was 20 674 head-times, and 590 schistosome infected cattle were found. The cumulative schistosomiasis treatment number of residents was 18739 person-times, and 512 cattle were treated. The cumulative detection area withJingxi City has reached the standard of schistosomiasis elimination, but there is still recurrence of

To investigate the epidemiology, diagnosis and treatment of an imported falciparum malaria patient in Xilin County, Baise City, so as to provide the reference for improving the diagnosis and treatment of falciparum malaria patients in the future.The epidemiological and clinical data were collected and analyzed.The patient had lived in Africa where the malaria was epidemic. The disease attacked him after his coming back home from abroad. The blood test forThe monitoring of overseas returnees in Xilin County should be strengthened to timely diagnose and treat the imported cases of malaria.

To explore the law and distribution characteristics of malaria prevalence in Pingguo County, Guangxi Zhuang Autonomous Region, so as to provide the evidence for formulating the targeted control strategy and measures after malaria elimination.The data of malaria epidemic situation in Pingguo County from 1951 to 2014 were collected and analyzed with the retrospective epidemiological method.From 1951 to 2014, there were 71 365 malaria cases reported in the county. The malaria incidence dropped from 429.43/10 000 in 1960 to 0 in 1993. Since 2002, there was no local endogenous infection case reported. In 1993, this county achieved the standard of malaria elimination basically, and in 2014, passed the examination and evaluation of malaria elimination.In Pingguo County, the comprehensive malarial control strategy is effective in different stages. In the future, the monitoring of mobile population and dealing with imported malaria cases timely is the key of consolidation of malaria control achievements.