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Additional file 2: Figure S2. Biotransformation of D-xylose to xylitol by cell extracts of (A & B) PsXYL1+GDH 4000-4 and (C & D) NcXR+GDH 4000-1 with or without formate. Enzyme reactions in (A & B) had 2.7 U of PsXYL1 activity while reactions in (C & D) had 12 U of NcXR activity. Cell extracts was incubated at 30oC with 200 mM D-xylose and 0.25 mM NAD+ in 50 mM KPi (pH 7.0) buffer. Formate (100 mM) was present only in the reactions in (A & C).

Additional file 3: Table S1. PCR primers used in this study.

Additional file 1: Figure S1. Biotransformation of D-xylose to xylitol by cell extracts of (A) NcXR+GDH 1000-2, (B) NcXR+GDH 4000-1, and (C) PsXYL1+GDH 4000-4. Enzyme reactions in (A) and (B) had 13 and 24 U of NcXR activity, respectively. Reaction in (C) had 5.4 U of PsXYL1 activity. Cell extracts was incubated at 30oC with 200 mM D-xylose, 100 mM glucose, and 0.25 mM NAD+ in 50 mM KPi (pH 7.0) buffer. Glycerol (10%, v/v) was added to all reactions for stabilizing enzyme activities. At 0 hr (black), 2 hr (blue), and 12 hr (red), samples were drawn from each reaction for HPLC analyses.