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Aims: We aimed to develop and test the psychometric properties of a Medical Device‐related Pressure Injuries Knowledge and Practice Assessment Tool. Background: Assessment of nurses' knowledge and practices is critical in the prevention of Medical Device‐related Pressure Injuries. Design: This was an instrument development and testing study. Methods: The sample of the study consisted of nurses (n = 189). The study was conducted in three phases between January and February 2021. In the first phase, multiple‐choice items contained within Aetiology/Risk Factors, Prevention Interventions, and Staging domains were created. In the second phase, content validity and criterion validity were evaluated, and the tool was pre‐tested. The third phase examined item difficulty, discrimination index and distractor quality. The test–retest method was used for reliability. Results: The Content Validity Index was found to be 0.75, 0.86 and 0.96 for the domains of Aetiology/Risk Factors, Prevention and Staging, respectively. The item difficulty values of the items were between 0.18 and 0.96. A positive, strong and significant relationship was found between the results and a positive, moderate and significant relationship between the tools administered for the proof of scale validity. The Cronbach's alpha reliability coefficient was found to be 0.54. Conclusions: The tool is a suitable measurement instrument for use in nursing education, research and clinical settings. Summary statement: What is already known about this topic? Medical Device‐related Pressure Injuries (MDRPIs) are a common and preventable issue in healthcare settings.Nurses' knowledge and practices are important in the prevention and management of MDRPIs.It is crucial to measure nurses' knowledge levels and practices on MDRPIs by using valid and reliable measurement tools. What this paper adds? The study developed and psychometrically tested a Medical Device‐related Pressure Injuries Knowledge and Practice Assessment Tool.The Medical Device‐related Pressure Injuries Knowledge and Practice Assessment Tool (MDPI‐ASSET) was shown to be a valid and reliable tool that can be used to evaluate knowledge and practices. The implications of this paper: The tool developed in this study can be adapted to different languages.The tool can be used in nursing practice, education and research to identify knowledge and practice gaps and focus areas on optimizing strategies to improve care quality. [ABSTRACT FROM AUTHOR]