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Humid heat and associated heat stress have increased in frequency, intensity, and duration across the globe, particularly at lower latitudes. One of the more robust metrics for heat stress impacts on the human body is wet-bulb globe temperature (WBGT), because it incorporates temperature, humidity, wind speed, and solar radiation. WBGT can typically only be measured using nonstandard instrumentation (e.g., black globe thermometers). However, estimation formulas have been developed to calculate WBGT using standard surface meteorological variables. This study evaluates several WBGT estimation formulas for the southeastern United States using North Carolina Environment and Climate Observing Network (ECONet) and U.S. Military measurement campaign data as verification. The estimation algorithm with the smallest mean absolute error was subsequently chosen to evaluate summer WBGT trends and extremes at 39 ASOS stations with long continuous (1950–2023) data records. Trend results showed that summer WBGT has increased throughout much of the southeastern United States, with larger increases at night than during the day. Although there were some surprisingly large WBGT trends at higher elevation locations far from coastlines, the greatest increases were predominantly located in the Florida Peninsula and Louisiana. Increases in the intensity and frequency of extreme (90th percentile) WBGTs were particularly stark in large coastal urban centers (e.g., New Orleans, Tampa, and Miami). Some locations like New Orleans and Tampa have experienced more than two additional extreme heat stress days and nights per decade since 1950, with an exponential escalation in the number of extreme summer nights during the most recent decade. Significance Statement: Humid heat and associated heat stress pose threats to health in the moist subtropical climate of the southeastern United States. Wet-bulb globe temperature (WBGT) is a robust metric for heat stress but must be estimated using complex algorithms. We first evaluated the accuracy of three WBGT algorithms in the southeastern United States, using measured verification data. Subsequently, we used the most accurate algorithm to investigate WBGT trends and extremes since 1950 in 39 cities. Results showed that summer heat stress has increased throughout the region, especially at night. Increases in the intensity and frequency of extreme heat stress were most prevalent at urban coastal locations in Florida and Louisiana, emphasizing the impacts of increased urbanization and evaporation on heat stress. [ABSTRACT FROM AUTHOR]