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6. Plant-Based Veterinary Vaccines

Author

Vighi, Isabel Lopes, de Quadros Ferreira, Kethlin, de Sousa, Guilherme Feijó, Dellagostin, Odir Antônio, da Silva Pinto, Luciano, Kole, Chittaranjan, Series Editor, Chaurasia, Anurag, editor, Hefferon, Kathleen L., editor, and Panigrahi, Jogeswar, editor

Published
2024
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9. An indirect ELISA to detect antibodies to the gC of bovine alphaherpesvirus 1 (BoAHV1) displaying no crossreactivity with antibodies induced by bovine alphaherpesvirus 5 (BoAHV5)

Author

Esteves, Paulo Augusto, Dellagostin, Odir Antonio, da Silva, Tamir Calcagnotto, Spilki, Fernando Rosado, da Silva, Alessandra D.’Ávila, Oliveira, Eber Acácio Stodutto, Franco, Ana Cláudia, Hübner, Silvia, Chiminazzo, Cláudio, Canal, Cláudio Wageck, Campos, Fabrício Souza, and Roehe, Paulo Michel

Published
2023
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10. Viral isolation allows characterization of early samples of SARS-CoV-2 lineage B1.1.33 with unique mutations (S: H655Y and T63N) circulating in Southern Brazil in 2020

Author

Gularte, Juliana Schons, da Silva, Mariana Soares, Filippi, Micheli, Demoliner, Meriane, Schallenberger, Karoline, Hansen, Alana Witt, de Abreu Góes Pereira, Vyctoria Malayhka, Heldt, Fágner Henrique, Girardi, Viviane, Weber, Matheus Nunes, de Almeida, Paula Rodrigues, Abbadi, Bruno Lopes, Dornelles, Maiele, Bizarro, Cristiano Valim, Machado, Pablo, Basso, Luiz Augusto, Dellagostin, Odir Antonio, Fleck, Juliane Deise, and Spilki, Fernando Rosado

Published
2022
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17. Time-dependent decay of detectable antibodies against SARS-CoV-2: A comparison of ELISA with two batches of a lateral-flow test

Author

Silveira, Mariangela F, Mesenburg, Marilia A, Dellagostin, Odir A, de Oliveira, Natasha R, Maia, Mara AC, Santos, Francisco D, Vale, André, Menezes, Ana M B, Victora, Gabriel D, Victora, Cesar G, Barros, Aluisio JD, Vidaletti, Luis Paulo, Hartwig, Fernando P, Barros, Fernando C, Hallal, Pedro C, and Horta, Bernardo L

Published
2021
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21. SARS-CoV-2 mutations in Brazil: from genomics to putative clinical conditions

Author

Timmers, Luis Fernando Saraiva Macedo, Peixoto, Julia Vasconcellos, Ducati, Rodrigo Gay, Bachega, José Fernando Ruggiero, de Mattos Pereira, Leandro, Caceres, Rafael Andrade, Majolo, Fernanda, da Silva, Guilherme Liberato, Anton, Débora Bublitz, Dellagostin, Odir Antônio, Henriques, João Antônio Pegas, Xavier, Léder Leal, Goettert, Márcia Inês, and Laufer, Stefan

Published
2021
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24. EPICOVID19 protocol: repeated serological surveys on SARS-CoV-2 antibodies in Brazil/Protocolo EPICOVID19: pesquisas sorologicas repetidas de anticorpos SARS-CoV-2 no Brasil

Author

Hallal, Pedro Curi, Barros, Fernando C., Silveira, Mariangela Freitas, Barros, Aluisio Jardim Dornellas de, Dellagostin, Odir Antonio, Pellanda, Lucia Campos, Struchiner, Claudio Jose, Burattini, Marcelo Nascimento, Hartwig, Fernando Pires, Menezes, Ana Maria Baptista, Horta, Bernardo Lessa, and Victora, Cesar Gomes

Published
2020
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25. Population-based surveys of antibodies against SARS-CoV-2 in Southern Brazil

Author

Silveira, Mariângela F., Barros, Aluísio J. D., Horta, Bernardo L., Pellanda, Lúcia C., Victora, Gabriel D., Dellagostin, Odir A., and Struchiner, Claudio J.

Subjects
Immunoglobulin M -- Identification and classification -- Methods, Epidemics -- Diagnosis -- Brazil, Sentinel health events -- Methods -- Diagnosis, Immunoglobulin G -- Identification and classification -- Methods, Biological sciences, Health
Abstract

Population-based data on COVID-19 are urgently needed. We report on three rounds of probability sample household surveys in the state of Rio Grande do Sul (Brazil), carried out in nine large municipalities using the Wondfo lateral flow point-of-care test for immunoglobulin M and G antibodies against SARS-CoV-2 ((https://en.wondfo.com.cn/product/wondfo-sars-cov-2-antibody-test-lateral-flow-method-2/)). Before survey use, the assay underwent four validation studies with pooled estimates of sensitivity (84.8%; 95% confidence interval (CI) = 81.4-87.8%) and specificity (99.0%; 95% CI = 97.8-99.7%). We calculated that the seroprevalence was 0.048% (2/4,151; 95% CI = 0.006-0.174) on 11-13 April (round 1), 0.135% (6/4,460; 95% CI = 0.049-0.293%) on 25-27 April (round 2) and 0.222% (10/4,500; 95% CI = 0.107-0.408) on 9-11 May (round 3), with a significant upward trend over the course of the surveys. Of 37 family members of positive individuals, 17 (35%) were also positive. The epidemic is at an early stage in the state, and there is high compliance with social distancing, unlike in other parts of Brazil. Periodic survey rounds will continue to monitor trends until at least the end of September, and our population-based data will inform decisions on preventive policies and health system preparedness at the state level. Three separate rounds of serological surveys in the state of Rio Grande do Sul in Brazil from mid-April to mid-May 2020 show an increase in antibodies specific for SARS-CoV-2 over time., Author(s): Mariângela F. Silveira [sup.1] , Aluísio J. D. Barros [sup.1] , Bernardo L. Horta [sup.1] , Lúcia C. Pellanda [sup.2] , Gabriel D. Victora [sup.3] , Odir A. Dellagostin [...]

Published
2020
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37. Cloning and expression of foreign genes in mycobacteria

Author

Dellagostin, Odir Antonio

Subjects
579, Tuberculosis, Foot-and-Mouth disease
Abstract

In this thesis, the construction and use of integrative vectors to express foreign genes in mycobacteria is described. These vectors are based on the transposition properties of the IS900 insertion sequence. Transformation by stable integration of multiple copies of the vector into the chromosome of Mycobacterium smegmatis, M. bovis BCG and M. vaccae was achieved. The M. leprae gene encoding the 18-kDa antigen was used to drive the expression of foreign antigens in mycobacteria. The major immunogenic site of Foot-and-Mouth disease virus (FMDV) comprising amino acids 140-160 of the virus protein 1 (VP1) was inserted in the 18kD gene and expressed as a fusion protein. Guinea pigs were vaccinated with the recombinant BCG expressing the FMDV epitope and immune responses specific for the FMDV were detected. A system for testing mycobacterial promoter strength in both extracellularly and intracellularly growing mycobacteria, as well as in E. coli was developed. It consists of a shuttle vector containing a promoterless reporter gene (lacZ). A promoter library was constructed using M. bovis BCG chromosomal DNA and screened in M. smegmatis and E. coli and a number of clones containing DNA inserts with promoter activity were isolated. The activity of each individual clone was measured in both, E. coli and M. smegmatis. Several previously characterized mycobacterial promoters including those of the Mycobacterium bovis BCG hsp60, the M. leprae 18kD and the putative iron regulated M. leprae 28kD gene were also cloned in front of the promoterless lacZ gene and the promoter strength was determined. To study intracellular expression, rBCG was used to infect murine macrophages and the activity of the reporter gene was measured using a fluorescent substrate and a FACS can system. Relative differences in the expression of the reporter gene during intracellular and extracellular growth were detected for some promoters. The BCG hsp60 promoter was shown to be the strongest of the characterized promoters also during intracellular growth. To obtain a higher level of expression of the 18kD/FMDV fusion, the 18kD promoter was replaced by the hsp60 promoter. A level of expression of the fused gene that in vitro was at least 10 fold higher was obtained with the hsp60 promoter in BCG.

Published
1994

43. Challenges for the development of a universal vaccine against leptospirosis revealed by the evaluation of 22 vaccine candidates

Author

Maia, Mara A. C., primary, Bettin, Everton B., additional, Barbosa, Liana N., additional, de Oliveira, Natasha R., additional, Bunde, Tiffany T., additional, Pedra, Ana Carolina K., additional, Rosa, Guilherme A., additional, da Rosa, Elias E. B., additional, Seixas Neto, Amilton C. P., additional, Grassmann, André A., additional, McFadden, Johnjoe, additional, Dellagostin, Odir A., additional, and McBride, Alan J. A., additional

Published
2022
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47. Proteomic analysis identifies differentially expressed proteins after red propolis treatment in Hep-2 cells

Author

da Silva Frozza, Caroline Olivieri, da Silva Ribeiro, Tanara, Gambato, Gabriela, Menti, Caroline, Moura, Sidnei, Pinto, Paulo Marcos, Staats, Charley Christian, Padilha, Francine Ferreira, Begnini, Karine Rech, Leon, Priscila Marques Moura de, Borsuk, Sibele, Savegnago, Lucielli, Dellagostin, Odir, Collares, Tiago, Seixas, Fabiana Kömmling, Henriques, João Antonio Pêgas, and Roesch-Ely, Mariana

Published
2014
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48. Identification of Proteins from Tuberculin Purified Protein Derivative (PPD) with Potential for TB Diagnosis Using Bioinformatics Analysis

Author

Borsuk, Sibele, Seixas, Fabiana Kommling, Ramos, Daniela Fernandes, Rizzi, Caroline, Dellagostin, Odir Antonio, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Nierstrasz, Oscar, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Sudan, Madhu, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Vardi, Moshe Y., Series editor, Weikum, Gerhard, Series editor, Istrail, Sorin, editor, Pevzner, Pavel, editor, Waterman, Michael S., editor, Guimarães, Katia S., editor, Panchenko, Anna, editor, and Przytycka, Teresa M., editor

Published
2009
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49. Use of transgenic Aedes aegypti in Brazil: risk perception and assessment/Utilisation d'une souche transgenique d'Aedes aegypti au Bresil: perception et evaluation des risques/Uso de Aedes aegypti transgenicos en Brasil: percepcion y evaluacion de riesgos

Author

de Andrade, Paulo Paes, Aragao, Francisco Jose Lima, Colli, Walter, Dellagostin, Odir Antonio, Finardi-Filho, Flavio, Hirata, Mario Fliroyuki, de Castro Lira-Neto, Amaro, de Melo, Marcia Almeida, Nepomuceno, Alexandre Lima, da Nobrega, Francisco Gorgonio, de Sousa, Gutemberg Delfino, Valicente, Fernando Hercos, and Zanettini, Maria Helena Bodanese

Subjects
Biosafety -- Health aspects, Mosquitoes -- Health aspects, Hostages -- Health aspects, Risk assessment -- Health aspects, Genetic engineering -- Health aspects, Disease transmission -- Health aspects, Proteins -- Health aspects, Genetically modified organisms -- Health aspects, Health, World Health Organization
Abstract

The OX513A strain of Aedes aegypti, which was developed by the British company Oxitec, expresses a self-limiting transgene that prevents larvae from developing to adulthood. In April 2014, the Brazilian National Technical Commission on Biosafety completed a risk assessment of 0X513A and concluded that the strain did not present new biological risks to humans or the environment and could be released in Brazil. At that point, Brazil became the first country to approve the unconstrained release of a genetically modified mosquito. During the assessment, the commission produced a comprehensive list of--and systematically analysed--the perceived hazards. Such hazards included the potential survival to adulthood of immature stages carrying the transgene--should the transgene fail to be expressed or be turned off by exposure to sufficient environmental tetracycline. Other perceived hazards included the potential allergenicity and/or toxicity of the proteins expressed by the gene, the potential for gene flow or increased transmission of human pathogens and the occupation of vacant breeding sites by other vector species. The Zika epidemic both elevated the perceived importance of Ae. aegypti as a vector--among policy-makers and regulators as well as the general public--and increased concerns over the release of males of the 0X513A strain. We have therefore reassessed the potential hazards. We found that release of the transgenic mosquitoes would still be both safe and of great potential value in the control of diseases spread by Ae aegypti, such as chikungunya, dengue and Zika. La souche OX513A d'Aedes aegypti, qui a ete creee par la societe britannique Oxitec, exprime un transgene autolimitant qui empeche les larves de se developper et de devenir adultes. En avril 2014, la Commission technique nationale de biosecurite du Bresil a procede a une evaluation des risques lies a la souche OX513A et conclu qu'elle ne presentait pas de nouveaux risques biologiques pour les etres humains ou l'environnement et pouvait etre lachee au Bresil. Le Bresil est donc devenu le premier pays a approuver le lacher non contraint d'un moustique genetiquement modifie. Au cours de l'evaluation, la commission a etabli une liste exhaustive des risques percus, qu'elle a par ailleurs systematiquement analyses. Ces risques incluaient la survie potentielle a l'age adulte des larves immatures porteuses du transgene--si le transgene ne s'exprime pas ou est desactive par une exposition a la tetracycline suffisante dans l'environnement. Les autres risques percus incluaient les potentielles proprietes allergisantes et/ ou la toxicite des proteines exprimees par le gene, l'eventualite d'un flux de genes ou d'une transmission accrue d'agents pathogenes pour l'homme et l'occupation de sites de reproduction vacants par d'autres especes vectrices. L'epidemie d'infections a virus Zika a accentue l'importance accordee par les responsables politiques, les organismes de reglementation ainsi que le grand public a Ae. aegypti en tant que moustique vecteur, et a accru l'Inquietude relative au lacher de males de la souche OX513A. Nous avons donc reevalue les risques potentiels. Nous estimons que le lacher de moustiques transgeniques serait a la fois sans danger et extremement utile pour lutter contre les maladies transmises par Ae. aegypti, telles que le chikungunya, la dengue et le virus Zika. La cepa OX513A de Aedes aegypti, que desarrollo la empresa britanica Oxltec, expresa un transgen autollmitado que Impide que las larvas se desarrollen hasta la edad adulta. En abril de 2014, la Comision Nacional Tecnica de Bioseguridad de Brasil realizo una evaluacion de riesgos de 0X513Ay concluyo que la cepa no presentaba nuevos riesgos biologicos para los humanos o el medloambiente y que podria liberarse en Brasil. En ese momento, Brasil se convirtio en el primer pais en aprobar la liberacion limitada de un mosquito modificado geneticamente. A lo largo de la evaluacion, la comision redacto una lista completa, y analizada sistematicamente, de las posibles contingencias. Entre dichos peligros se encontraba la posible supervivencia hasta la edad adulta de etapas inmaduras que portan el transgen, en caso de que este no consiga expresarse o se inutilice debido a la exposicion a la suficiente tetraciclina medioambiental. Otras posibles contingencias eran la alergia y/o toxicidad de las proteinas expresadas por el gen, la posibilidad de un flujo genetico o el aumento de la transmision de patogenos humanos y la ocupacion de lugares de cria desocupados por parte de otras especies vectores. La epidemia por el virus de Zlka aumento la importancia de Ae. aegypti como vector, entre los responsables y reguladores politicos, asi como entre el publico general, y aumento las preocupaciones acerca de la liberacion de machos de la cepa 0X513A. Por lo tanto, se han vuelto a evaluar los posibles riesgos. Se ha descubierto que la liberacion de mosquitos transgenicos seria segura y tendria un gran valor potencial en el control de la propagacion de enfermedades por Ae aegypti, como la fiebre chlkungunya, el dengue y la enfermedad por el virus de Zika., Introduction In April 2014, Brazil's National Technical Commission on Biosafety--the agency officially responsible for the assessment of the risks posed by genetically modified organisms in Brazil--assessed the potential risks of [...]

Published
2016
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