Search

Your search keyword '"Dei Dei"' showing total 3 results
Start Over Author "Dei Dei"
3 results on '"Dei Dei"'

1. BL-MOL-AR Project, Preliminary Results about Liquid Biopsy: Molecular Approach Experience and Research Activity in Oncological Settings

Author

Pancrazzi Pancrazzi, Bloise Bloise, Moncada Moncada, Perticucci Perticucci, Vecchietti Vecchietti, Pompili Pompili, Ricciarini Ricciarini, Lenzi Lenzi, Gatteschi Gatteschi, Giusti Giusti, Rosito Rosito, Del Buono Del Buono, Belardi Belardi, Bruni Bruni, Borri Borri, Campione Campione, Laurini Laurini, Occhini Occhini, Presenti Presenti, Viticchi Viticchi, Rossi Rossi, Bardi Bardi, D'Urso D'Urso, Dei Dei, Venezia Venezia, Scala Scala, Bengala Bengala, Decarli Decarli, Carnevali Carnevali, Milandri Milandri, and Ognibene Ognibene

Subjects
liquid biopsy, cfDNA, NGS, Genetics, QH426-470, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Background Liquid biopsy is mainly used to identify tumor cells in pulmonary neoplasms. It is more often used in research than in clinical practice. The BL-MOL-AR study aims to investigate the efficacy of next-generation sequencing (NGS) and clinical interpretation of the circulating free DNA (cfDNA) levels. This study reports the preliminary results from the first samples analyzed from patients affected by various neoplasms: lung, intestinal, mammary, gastric, biliary, and cutaneous.

Published
2023
Full Text
View/download PDF

2. Spns2 Transporter Contributes to the Accumulation of S1P in Cystic Fibrosis Human Bronchial Epithelial Cells

Author

Zulueta, Aida, Cas, Michele Dei Dei, Luciano, Francesco, Mingione, Alessandra, Pivari, Francesca, Righi, Ilaria, Morlacchi, Letizia, Rosso, Lorenzo, Signorelli, Paola, Ghidoni, Riccardo, Paroni, Rita, and Caretti, Anna

Subjects
cystic fibrosis, Spns2, sphingosine, QH301-705.5, lipids (amino acids, peptides, and proteins), SGPL1, sphingolipid, Biology (General), SphK1, S1P, Article, lung
Abstract

The role of S1P in Cystic Fibrosis (CF) has been investigated since 2001, when it was first described that the CFTR channel regulates the inward transport of S1P. From then on, various studies have associated F508del CFTR, the most frequent mutation in CF patients, with altered S1P expression in tissue and plasma. We found that human bronchial epithelial immortalized and primary cells from CF patients express more S1P than the control cells, as evidenced by mass spectrometry analysis. S1P accumulation relies on two- to four-fold transcriptional up-regulation of SphK1 and simultaneous halving of SGPL1 in CF vs. control cells. The reduction of SGPL1 transcription protects S1P from irreversible degradation, but the excessive accumulation is partially prevented by the action of the two phosphatases that are up-regulated compared to control cells. For the first time in CF, we describe that Spns2, a non-ATP dependent transporter that normally extrudes S1P out of the cells, shows deficient transcriptional and protein expression, thus impairing S1P accrual dissipation. The in vitro data on CF human bronchial epithelia correlates with the impaired expression of Spns2 observed in CF human lung biopsies compared to healthy control.

Published
2021

3. Production of transgenic mice expressing the Ki-ras oncogene under the control of a thyroglobulin promoter.

Author

Santelli G, de Franciscis V, Portella G, Chiappetta G, D'Alessio A, Califano D, Rosati R, Mineo A, Monaco C, and Manzo G

Subjects
Adenoma enzymology, Adenoma pathology, Amitrole pharmacology, Animals, Base Sequence, Chloramphenicol O-Acetyltransferase analysis, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, In Situ Hybridization, Mice, Molecular Sequence Data, Perchlorates pharmacology, Polymerase Chain Reaction, RNA, Messenger analysis, Sodium Compounds pharmacology, Thyroid Gland drug effects, Thyroid Gland enzymology, Thyroid Gland pathology, Thyroid Neoplasms enzymology, Thyroid Neoplasms pathology, Adenoma genetics, Chloramphenicol O-Acetyltransferase genetics, Genes, ras, Mice, Transgenic genetics, Thyroid Neoplasms genetics
Abstract

Transgenic mice have been generated bearing three fusion genes consisting of: (a) a 900-base pair rat thyroglobulin promoter followed by a gene coding for a chloramphenicol acetyl transferase activity; (b) the same promoter followed by the complementary DNA of the human activated Ki-ras oncogene; (c) a 2000-base pair rat thyroglobulin promoter followed by the complementary DNA of the human activated Ki-ras. We have shown that the 900-base pair rat thyroglobulin promoter is able to direct the expression of the reporter gene specifically in the thyroid gland of transgenic mice. The mice bearing the two Ki-ras constructs, which express the transgene in thyroid glands, show thyroid abnormalities, although at very low incidence. These lesions appear after a long latency and with a benign aspect, thus suggest that, in agreement with literature data on naturally occurring human thyroid tumors, the action of an activated ras gene is not sufficient to attain a complete malignant conversion of thyroid glands in vivo. However, ras expression in thyroid follicular cells represents a favorable ground for tumor development, as shown by the fact that goitrogen stimulation experiments increase the occurrence of tumors.

Published
1993

Catalog

Books, media, physical & digital resources
See catalog results