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3,324 results on '"Deffieux A"'

1. The urotensin II receptor triggers an early meningeal response and a delayed macrophage-dependent vasospasm after subarachnoid hemorrhage in male mice

Author

Martin Pedard, Lucie Prevost, Camille Carpena, Brian Holleran, Laurence Desrues, Martine Dubois, Celeste Nicola, Roxane Gruel, David Godefroy, Thomas Deffieux, Mickael Tanter, Carine Ali, Richard Leduc, Laurent Prézeau, Pierrick Gandolfo, Fabrice Morin, Olivier Wurtz, Thomas Bonnard, Denis Vivien, and Hélène Castel

Subjects
Science
Abstract

Abstract Subarachnoid hemorrhage (SAH) can be associated with neurological deficits and has profound consequences for mortality and morbidity. Cerebral vasospasm (CVS) and delayed cerebral ischemia affect neurological outcomes in SAH patients, but their mechanisms are not fully understood, and effective treatments are limited. Here, we report that urotensin II receptor UT plays a pivotal role in both early events and delayed mechanisms following SAH in male mice. Few days post-SAH, UT expression is triggered by blood or hemoglobin in the leptomeningeal compartment. UT contributes to perimeningeal glia limitans astrocyte reactivity, microvascular alterations and neuroinflammation independent of CNS-associated macrophages (CAMs). Later, CAM-dependent vascular inflammation and subsequent CVS develop, leading to cognitive dysfunction. In an SAH model using humanized UTh+/h+ male mice, we show that post-SAH CVS and behavioral deficits, mediated by UT through Gq/PLC/Ca2+ signaling, are prevented by UT antagonists. These results highlight the potential of targeting UT pathways to reduce early meningeal response and delayed cerebral ischemia in SAH patients.

Published
2024
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2. The urotensin II receptor triggers an early meningeal response and a delayed macrophage-dependent vasospasm after subarachnoid hemorrhage in male mice

Author

Pedard, Martin, Prevost, Lucie, Carpena, Camille, Holleran, Brian, Desrues, Laurence, Dubois, Martine, Nicola, Celeste, Gruel, Roxane, Godefroy, David, Deffieux, Thomas, Tanter, Mickael, Ali, Carine, Leduc, Richard, Prézeau, Laurent, Gandolfo, Pierrick, Morin, Fabrice, Wurtz, Olivier, Bonnard, Thomas, Vivien, Denis, and Castel, Hélène

Published
2024
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3. Toward Whole-Brain Minimally-Invasive Vascular Imaging

Author

Jimenez, Anatole, Osmanski, Bruno, Vivien, Denis, Tanter, Mickael, Gaberel, Thomas, and Deffieux, Thomas

Subjects
Quantitative Biology - Neurons and Cognition, Electrical Engineering and Systems Science - Image and Video Processing
Abstract

Imaging the brain vasculature can be critical for cerebral perfusion monitoring in the context of neurocritical care. Although ultrasensitive Doppler (UD) can provide good sensitivity to cerebral blood volume (CBV) in a large field of view, it remains difficult to perform through the skull. In this work, we investigate how a minimally invasive burr hole, performed for intracranial pressure (ICP) monitoring, could be used to map the entire brain vascular tree. We explored the use of a small motorized phased array probe with a non-implantable preclinical prototype in pigs. The scan duration (18 min) and coverage (62 $\pm$ 12 % of the brain) obtained allowed global CBV variations detection (relative in brain Dopplerdecrease =-3[-4-+16]% \& Dopplerincrease. = +1[-3-+15]%, n = 6 \& 5) and stroke detection (relative in core Dopplerstroke. =-25%, n = 1). This technology could one day be miniaturized to be implanted for brain perfusion monitoring in neurocritical care.

Published
2023

6. Prospective head-to-head comparison of non-invasive scores for diagnosis of fibrotic MASH in patients with type 2 diabetes

Author

Bellili, Djamila, Bessadi, Ouarda, Da Silveira, Charlene, Djelouat, Fatima Zohra, Girard, Benoit, Legrand, Vanessa, Neveux, Nathalie, Meziani, Meriam, Roy, Ludovic, Sekour, Dahia, Sens, Manon, Slimani, Miassa, Zatout, Ouassila, Bachelet, Delphine, Bhavsar, Krishna, Mullaert, Basma Basli-Baillet Jimmy, Marcault, Estelle, Si-Mohammed, Nassima, Cosson, Emmanuel, Miguel Albuquerque, Doblas, Sabrina, Hammoutene, Adel, Gonzalez Montpetit, Estefania, Pagé, Gwenaël, Béatrice Parfait, Postic, Catherine, Lehuen, Agnès, Toubal, Amine, Rousseau, Camille, Fruchet, Blandine, Soulard, Pauline, Gouda, Zouriatou, Vidaud, Michel, Letourneur, Franck, Renault, Gilles, Scharfmann, Raphaël, Ait-Boudaoud, Amel, Barsamian, Charles, Carette, Claire, Rives-Lange, Claire, Baida, Rachel, Couture, Olivier, Decombas, Sofiane, Deffieux, Thomas, Nguyen, Thu-mai, Tanter, Mickael, Baltauss, Tania, Balzac, Edwige-Ludiwyne, Barbier Saint Hilaire, Pierre, Delerive, Philippe, Duvivier, Valérie, Fillon, Arnaud, Geronimi, Julia, Laplume, Jessica, Werner, Erwan, Xuereb, Laura, Liechti, Robin, Martin, Olivier, Mehl, Florence, Pruess, Manuela, Castille, Jean-Marie, Drane, Fabienne, Deckmyn, Olivier, Castelli, Florence, Cousin, Benoit Colsch Emmanuel, Fenaille, François, Guilbaud, Laure, Lohier, Allyre, Chambellin, Francois, Laaland, Lyddie, Clusel, Catherine, Hauduroy, Marie, Pautre, Pierre, Castera, Laurent, Garteiser, Philippe, Laouenan, Cédric, Vidal-Trécan, Tiphaine, Vallet-Pichard, Anaïs, Manchon, Pauline, Paradis, Valérie, Czernichow, Sébastien, Roulot, Dominique, Larger, Etienne, Pol, Stanislas, Bedossa, Pierre, Correas, Jean-Michel, Valla, Dominique, Gautier, Jean-François, and Van Beers, Bernard E.

Published
2024
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8. Leptomeningeal collaterals regulate reperfusion in ischemic stroke and rescue the brain from futile recanalization

Author

Binder, Nadine Felizitas, El Amki, Mohamad, Glück, Chaim, Middleham, William, Reuss, Anna Maria, Bertolo, Adrien, Thurner, Patrick, Deffieux, Thomas, Lambride, Chryso, Epp, Robert, Handelsmann, Hannah-Lea, Baumgartner, Philipp, Orset, Cyrille, Bethge, Philipp, Kulcsar, Zsolt, Aguzzi, Adriano, Tanter, Mickael, Schmid, Franca, Vivien, Denis, Wyss, Matthias Tasso, Luft, Andreas, Weller, Michael, Weber, Bruno, and Wegener, Susanne

Published
2024
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10. Diagnosis and management of complications following pelvic organ prolapse surgery using a synthetic mesh: French national guidelines for clinical practice

Author

Deffieux, Xavier, Perrouin-Verbe, Marie-Aimée, Campagne-Loiseau, Sandrine, Donon, Laurence, Levesque, Amélie, Rigaud, Jérome, Stivalet, Nadja, Venara, Aurélien, Thubert, Thibault, Vidart, Adrien, Bosset, Pierre-Olivier, Revel-Delhom, Christine, Lucot, Jean-Philippe, and Hermieu, Jean François

Published
2024
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14. Pelvic exam in Gynecology and Obstetrics: French Guidelines for Clinical Practice

Author

Deffieux, Xavier, Pizzoferrato, Anne-Cécile, Gaucher, Laurent, Rousset-Jablonski, Christine, Le Ray, Camille, Brillac, Thierry, Maruani, Julia, Maitrot-Mantelet, Lorraine, Mignot, Stéphanie, Athiel, Yoann, Baffet, Hortense, Bailleul, Alexandre, Bernard, Valérie, Bourdon, Mathilde, Cardaillac, Claire, Carneiro, Yaritza, Chariot, Patrick, Corroenne, Romain, Dabi, Yohann, Dahlem, Laurence, Frank, Sophie, Freyens, Anne, Grouthier, Virginie, Hernandez, Isabelle, Iraola, Elisabeth, Lambert, Marie, Lauchet, Nadege, Legendre, Guillaume, Le Lous, Maela, Louis-Vahdat, Christine, Martinat Sainte-Beuve, Anne, Masson, Marine, Matteo, Caroline, Pinton, Anne, Sabbagh, Emmanuelle, Sallee, Camille, Thubert, Thibault, Heron, Isabelle, Artzner, France, Tavenet, Arounie, Gantois, Adrien, and Fauconnier, Arnaud

Published
2023
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16. Réunion de concertation pluridisciplinaire en pelvipérinéologie en France : résultats d’une enquête nationale du CUROPF auprès des urologues français

Author

Dupuis, Hugo G.A., Berrogain, Nathalie, Bosset, Pierre-Olivier, Campagne-Loiseau, Sandrine, Cardot, Vincent, Charles, Thomas, Deffieux, Xavier, Donon, Laurence, Even, Lucie, Girard, Frédéric, Hermieu, Jean-François, Hurel, Sophie, Klap, Julia, Peyrat, Laurence, Meyer, François, Peyronnet, Benoît, Tibi, Brannwel, Thuillier, Caroline, Vidart, Adrien, Wagner, Laurent, and Cornu, Jean-Nicolas

Published
2023
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17. The SVD Beamformer: Physical Principles and Application to Ultrafast Adaptive Ultrasound

Author

Bendjador, Hanna, Deffieux, Thomas, and Tanter, Mickaël

Subjects
Electrical Engineering and Systems Science - Image and Video Processing
Abstract

A shift of paradigm is currently underway in biomedical ultrasound thanks to plane and diverging waves for ultrafast imaging. One remaining challenge consists in the correction of phase and amplitude aberrations induced during propagation through complex layers. Unlike conventional line-per-line imaging, ultrafast ultrasound provides for each transmission, backscattering information from the whole imaged area. Here, we take benefit from this feature and propose an efficient approach to perform fast aberration correction based on the Singular Value Decomposition of an ultrafast compound matrix built from backscattered data for several plane wave transmissions. First, we explain the physical signification of SVD and associated singular vectors within the ultrafast matrix formalism. We theoretically demonstrate that the spatial and angular variables separation, rendered by SVD on ultrafast data, provides an elegant and straightforward way to optimize angular coherence of backscattered data. In heterogeneous media with an aberrating phase screen approximation, we demonstrate that the first spatial and angular singular vectors retrieve on one side the non-aberrated image, and on the other, the phase and amplitude of the aberration law. In vitro results prove the efficiency of the image correction, but also the accuracy of the aberrator determination. Based on spatial and angular coherence, we introduce a complete methodology for adaptive beamforming of ultrafast data, performed on successive isoplanatism patches undergoing SVD beamforming. The simplicity of this method paves the way to real-time adaptive ultrafast ultrasound imaging and provides a theoretical framework for future quantitative ultrasound applications.

Published
2019

18. Examen pelvien en gynécologie et obstétrique : recommandations pour la pratique clinique

Author

Deffieux, Xavier, Rousset-Jablonski, Christine, Gantois, Adrien, Brillac, Thierry, Maruani, Julia, Maitrot-Mantelet, Lorraine, Mignot, Stéphanie, Gaucher, Laurent, Athiel, Yoann, Baffet, Hortense, Bailleul, Alexandre, Bernard, Valérie, Bourdon, Mathilde, Cardaillac, Claire, Carneiro, Yaritza, Chariot, Patrick, Corroenne, Romain, Dabi, Yohann, Dahlem, Laurence, Frank, Sophie, Freyens, Anne, Grouthier, Virginie, Hernandez, Isabelle, Iraola, Elisabeth, Lambert, Marie, Lauchet, Nadege, Legendre, Guillaume, Le Lous, Maela, Louis-Vahdat, Christine, Martinat Sainte-Beuve, Anne, Masson, Marine, Matteo, Caroline, Pinton, Anne, Sabbagh, Emmanuelle, Sallee, Camille, Thubert, Thibault, Heron, Isabelle, Pizzoferrato, Anne-Cécile, Artzner, France, Tavenet, Arounie, Le Ray, Camille, and Fauconnier, Arnaud

Published
2023
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19. Retinal functional ultrasound imaging (rfUS) for assessing neurovascular alterations: a pilot study on a rat model of dementia

Author

Clementine Morisset, Alexandre Dizeux, Benoit Larrat, Erwan Selingue, Herve Boutin, Serge Picaud, Jose-Alain Sahel, Nathalie Ialy-Radio, Sophie Pezet, Mickael Tanter, and Thomas Deffieux

Subjects
Medicine, Science
Abstract

Abstract Fifty million people worldwide are affected by dementia, a heterogeneous neurodegenerative condition encompassing diseases such as Alzheimer’s, vascular dementia, and Parkinson’s. For them, cognitive decline is often the first marker of the pathology after irreversible brain damage has already occurred. Researchers now believe that structural and functional alterations of the brain vasculature could be early precursors of the diseases and are looking at how functional imaging could provide an early diagnosis years before irreversible clinical symptoms. In this preclinical pilot study, we proposed using functional ultrasound (fUS) on the retina to assess neurovascular alterations non-invasively, bypassing the skull limitation. We demonstrated for the first time the use of functional ultrasound in the retina and applied it to characterize the retinal hemodynamic response function in vivo in rats following a visual stimulus. We then demonstrated that retinal fUS could measure robust neurovascular coupling alterations between wild-type rats and TgF344-AD rat models of Alzheimer’s disease. We observed an average relative increase in blood volume of 21% in the WT versus 37% for the TG group (p = 0.019). As a portable, non-invasive and inexpensive technique, rfUS is a promising functional screening tool in clinics for dementia years before symptoms.

Published
2022
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24. Perceived health status after mid‐urethral sling revision in 287 women from the VIGI‐MESH registry: A cross‐sectional study

Author

Camilli, Hugo, primary, Fatton, Brigitte, additional, Gand, Elise, additional, Campagne‐Loiseau, Sandrine, additional, De Tayrac, Renaud, additional, Wagner, Laurent, additional, Saussine, Christian, additional, Rigaud, Jérôme, additional, Thubert, Thibault, additional, Deffieux, Xavier, additional, Cosson, Michel, additional, Ferry, Philippe, additional, Capon, Grégoire, additional, Panel, Laure, additional, Chartier‐Kastler, Emmanuel, additional, Gauthier, Tristan, additional, Game, Xavier, additional, Bouynat, Cassandra, additional, Bichon, Romane, additional, Fauconnier, Arnaud, additional, Pizzoferrato, Anne‐Cécile, additional, and Fritel, Xavier, additional

Published
2024
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25. Preparation of a ε-caprolactonic diterpenoid derivate by unexpected oxidative cleavage/lactonization of 2-oxoaustroeupatol

Author

Pablo A. Chacón-Morales, Juan M. Amaro-Luis, Luis Beltrán Rojas Fermín, Rémi Jacquet, Denis Deffieux, Laurent Pouységu, and Stéphane Quideau

Subjects
Oxidative cleavage, Austroeupatol, NaIO4, IBX, Lactonization, NMR, Botany, QK1-989
Abstract

Abstract From aerial parts of Austroeupatorium inulifolium was isolated the ent-nor-furano triol labdane austroeupatol 1. The compound 1 was treated with IBX showing an unexpected selectivity at the potentially oxidizable sites of the substrate yielding the 2-oxoaustroeupatol (2) and 2,19-dioxoaustroeupatol (3). The treatment of 2 with sodium periodate yields a heterocyclic derivative (ε-caprolactone derivate 4) formed by oxidative cleavage and unexpected intramolecular attack of the hydroxymethylene (C-19) oxygen to the ketonic carbon (C-2). A plausible mechanistic pathway for the obtention of compound 4 is proposed. Graphical Abstract

Published
2022
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26. Co-variations of cerebral blood volume and single neurons discharge during resting state and visual cognitive tasks in non-human primates

Author

Julien Claron, Matthieu Provansal, Quentin Salardaine, Pierre Tissier, Alexandre Dizeux, Thomas Deffieux, Serge Picaud, Mickael Tanter, Fabrice Arcizet, and Pierre Pouget

Subjects
CP: Neuroscience, Biology (General), QH301-705.5
Abstract

Summary: To better understand how the brain allows primates to perform various sets of tasks, the ability to simultaneously record neural activity at multiple spatiotemporal scales is challenging but necessary. However, the contribution of single-unit activities (SUAs) to neurovascular activity remains to be fully understood. Here, we combine functional ultrasound imaging of cerebral blood volume (CBV) and SUA recordings in visual and fronto-medial cortices of behaving macaques. We show that SUA provides a significant estimate of the neurovascular response below the typical fMRI spatial resolution of 2mm3. Furthermore, our results also show that SUAs and CBV activities are statistically uncorrelated during the resting state but correlate during tasks. These results have important implications for interpreting functional imaging findings while one constructs inferences of SUA during resting state or tasks.

Published
2023
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33. Ultrafast Doppler imaging and ultrasound localization microscopy reveal the complexity of vascular rearrangement in chronic spinal lesion

Author

Benoit Beliard, Chaimae Ahmanna, Elodie Tiran, Kadia Kanté, Thomas Deffieux, Mickael Tanter, Fatiha Nothias, Sylvia Soares, and Sophie Pezet

Subjects
Medicine, Science
Abstract

Abstract Acute spinal cord injury (SCI) leads to severe damage to the microvascular network. The process of spontaneous repair is accompanied by formation of new blood vessels; their functionality, however, presumably very important for functional recovery, has never been clearly established, as most studies so far used fixed tissues. Here, combining ultrafast Doppler imaging and ultrasound localization microscopy (ULM) on the same animals, we proceeded at a detailed analysis of structural and functional vascular alterations associated with the establishment of chronic SCI, both at macroscopic and microscopic scales. Using a standardized animal model of SCI, our results demonstrate striking hemodynamic alterations in several subparts of the spinal cord: a reduced blood velocity in the lesion site, and an asymmetrical hypoperfusion caudal but not rostral to the lesion. In addition, the worsening of many evaluated parameters at later time points suggests that the neoformed vascular network is not yet fully operational, and reveals ULM as an efficient in vivo readout for spinal cord vascular alterations. Finally, we show statistical correlations between the diverse biomarkers of vascular dysfunction and SCI severity. The imaging modality developed here will allow evaluating recovery of vascular function over time in pre-clinical models of SCI. Also, used on SCI patients in combination with other quantitative markers of neural tissue damage, it may help classifying lesion severity and predict possible treatment outcomes in patients.

Published
2022
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34. Ultrasound localization microscopy and functional ultrasound imaging reveal atypical features of the trigeminal ganglion vasculature

Author

Annabelle Réaux-Le-Goazigo, Benoit Beliard, Lauriane Delay, Line Rahal, Julien Claron, Noémi Renaudin, Isabelle Rivals, Miguel Thibaut, Mohamed Nouhoum, Thomas Deffieux, Mickael Tanter, and Sophie Pezet

Subjects
Biology (General), QH301-705.5
Abstract

Visualisation of rat trigeminal ganglia activation during ophthalmic or maxillary nociceptive stimulations shows atypical tortuous vascularisation and a somatotopic hemodynamic response.

Published
2022
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37. Serious complications and recurrences after retropubic vs transobturator midurethral sling procedures for 2682 patients in the VIGI-MESH register

Author

Armengaud, Camille, Fauconnier, Arnaud, Drioueche, Hocine, Campagne Loiseau, Sandrine, De Tayrac, Renaud, Saussine, Christian, Panel, Laure, Cosson, Michel, Deffieux, Xavier, Lucot, Jean Philippe, Pizzoferrato, Anne Cécile, Ferry, Philippe, Vidart, Adrien, Thubert, Thibault, Capon, Grégoire, Debodinance, Philippe, Gauthier, Tristan, Koebele, Antoine, Salet-Lizee, Delphine, Hermieu, Jean-François, Game, Xavier, Ramanah, Rajeev, Lamblin, Gery, Lecornet, Emilie, Carlier-Guérin, Caroline, Chartier-Kastler, Emmanuel, and Fritel, Xavier

Published
2024
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38. Complications des bandelettes sous urétrales : une revue de la littérature par le Comité d’urologie et pelvipérinéologie de la femme de l’Association française d’urologie

Author

Even, L., Tibi, B., Bentellis, I., Treacy, P.J., Berrogain, N., Bosset, P.O., Campagne-Loiseau, S., Cardot, V., Charles, T., Deffieux, X., Donon, L., Girard, F., Hermieu, J.-F., Hurel, S., Klap, J., Meyer, F., Peyrat, L., Thuillier, C., Vidart, A., Wagner, L., and Cornu, J.N.

Published
2021
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40. Prospective head-to-head comparison of non-invasive scores for diagnosis of fibrotic MASH in patients with type 2 diabetes

Author

Castera, Laurent, primary, Garteiser, Philippe, additional, Laouenan, Cédric, additional, Vidal-Trécan, Tiphaine, additional, Vallet-Pichard, Anaïs, additional, Manchon, Pauline, additional, Paradis, Valérie, additional, Czernichow, Sébastien, additional, Roulot, Dominique, additional, Larger, Etienne, additional, Pol, Stanislas, additional, Bedossa, Pierre, additional, Correas, Jean-Michel, additional, Valla, Dominique, additional, Gautier, Jean-François, additional, Van Beers, Bernard E., additional, Bellili, Djamila, additional, Bessadi, Ouarda, additional, Da Silveira, Charlene, additional, Djelouat, Fatima Zohra, additional, Girard, Benoit, additional, Legrand, Vanessa, additional, Neveux, Nathalie, additional, Meziani, Meriam, additional, Roy, Ludovic, additional, Sekour, Dahia, additional, Sens, Manon, additional, Slimani, Miassa, additional, Zatout, Ouassila, additional, Bachelet, Delphine, additional, Bhavsar, Krishna, additional, Basli-Baillet Jimmy Mullaert, Basma, additional, Marcault, Estelle, additional, Si-Mohammed, Nassima, additional, Cosson, Emmanuel, additional, Albuquerque, Miguel, additional, Doblas, Sabrina, additional, Hammoutene, Adel, additional, Montpetit, Estefania Gonzalez, additional, Pagé, Gwenaël, additional, Parfait, Béatrice, additional, Postic, Catherine, additional, Lehuen, Agnès, additional, Toubal, Amine, additional, Rousseau, Camille, additional, Fruchet, Blandine, additional, Soulard, Pauline, additional, Gouda, Zouriatou, additional, Vidaud, Michel, additional, Letourneur, Franck, additional, Renault, Gilles, additional, Scharfmann, Raphaël, additional, Ait-Boudaoud, Amel, additional, Barsamian, Charles, additional, Carette, Claire, additional, Rives-Lange, Claire, additional, Baida, Rachel, additional, Couture, Olivier, additional, Decombas, Sofiane, additional, Deffieux, Thomas, additional, Nguyen, Thu-mai, additional, Tanter, Mickael, additional, Baltauss, Tania, additional, Balzac, Edwige-Ludiwyne, additional, Saint Hilaire Philippe Delerive, Pierre Barbier, additional, Duvivier, Valérie, additional, Fillon, Arnaud, additional, Geronimi, Julia, additional, Laplume, Jessica, additional, Werner, Erwan, additional, Xuereb, Laura, additional, Liechti, Robin, additional, Martin, Olivier, additional, Mehl, Florence, additional, Pruess, Manuela, additional, Castille, Jean-Marie, additional, Drane, Fabienne, additional, Deckmyn, Olivier, additional, Castelli, Florence, additional, Emmanuel Cousin, Benoit Colsch, additional, Fenaille, François, additional, Guilbaud, Laure, additional, Lohier, Allyre, additional, Chambellin, Francois, additional, Laaland, Lyddie, additional, Clusel, Catherine, additional, Hauduroy, Marie, additional, and Pautre, Pierre, additional

Published
2024
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41. Recommandations pour Pratique Clinique du Collège des Gynécologues Obstétriciens français 2021 : gestes prophylactiques associés en chirurgie gynécologique

Author

Touboul, C., Legendre, G., Agostini, A., Akladios, C., Bendifallah, S., Bolze, P.A., Bouet, P.E., Chauvet, P., Collinet, P., Dabi, Y., Delotte, J., Deffieux, X., Dion, L., Gauthier, T., Kerbage, Y., Koskas, M., Millet, P., Narducci, F., Ouldamer, L., Ploteau, S., Santulli, P., and Golfier, F.

Published
2021
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50. Synthesis of Flavonol-Bearing Probes for Chemoproteomic and Bioinformatic Analyses of Asteraceae Petals in Search of Novel Flavonoid Enzymes

Author

Karl Kempf, Oxana Kempf, Yoan Capello, Christian Molitor, Claire Lescoat, Rana Melhem, Stéphane Chaignepain, Elisabeth Génot, Alexis Groppi, Macha Nikolski, Heidi Halbwirth, Denis Deffieux, and Stéphane Quideau

Subjects
plant polyphenols, Asteraceae UV-honey guides, higher hydroxylated flavonols, flavonoid biosynthesis, chemoproteomics, bioinformatics, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

This study aimed at searching for the enzymes that are responsible for the higher hydroxylation of flavonols serving as UV-honey guides for pollinating insects on the petals of Asteraceae flowers. To achieve this aim, an affinity-based chemical proteomic approach was developed by relying on the use of quercetin-bearing biotinylated probes, which were thus designed and synthesized to selectively and covalently capture relevant flavonoid enzymes. Proteomic and bioinformatic analyses of proteins captured from petal microsomes of two Asteraceae species (Rudbeckia hirta and Tagetes erecta) revealed the presence of two flavonol 6-hydroxylases and several additional not fully characterized proteins as candidates for the identification of novel flavonol 8-hydroxylases, as well as relevant flavonol methyl- and glycosyltransferases. Generally speaking, this substrate-based proteome profiling methodology constitutes a powerful tool for the search for unknown (flavonoid) enzymes in plant protein extracts.

Published
2023
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