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Introduction: Programmed cell death (PCD) is a fundamental biological process crucial for plant development. Despite recent advancements in our understanding of PCD's molecular mechanisms, the intricate orchestration of this process within plant cells remains enigmatic. To address this knowledge gap, the present study focuses on Decaisnea insignis, a plant species renowned for its unique fruit anatomy, including laticiferous canals that secrete latex. While extensive anatomical studies have elucidated the structural features of these canals,molecular insights into their developmental regulation, particularly the involvement of PCD, are lacking. Methods: In this study, we sequenced the single-cell transcriptomes at two developmental stage of Decaisnea insignis fruit using the technology known as 10x Genomics (S1, S2). Using sequencing technology combining full- length RNA sequencing and single-nucleus RNA sequencing (snRNA-seq) in combination with ultrastructural analyses, our study revealed a cellular map of Decaisnea insignis fruit at the single-cell level and identified different cell types. Results: In particular, we identified a possible PCD-mediated cluster of Decaisnea insignis fruit lactiferous canals in epidermal cells and clarified the expression patterns of DiRD21A (a hydrolase) and DiLSD1 (a transcription factor), which may be closely related to the development of laticiferous canals in Decaisnea insignis fruits. Discussion: By integrating high-resolution gene expression profiling with visual insights into cellular transformations, we sought to more precisely characterize the regulatory role of PCD during the developmental formation of lactiferous canals in Decaisnea insignis fruit. [ABSTRACT FROM AUTHOR]

Natural rubber is one of the most important industrial raw materials, and its biosynthesis is still a fascinating process that is still largely unknown. In this research, we studied Decaisnea insignis, a unique rubber-producing plant that is different from other rubber-producing species due to the presence of lactiferous canals in its pericarp. The present study aims to provide novel insights into the mechanisms underlying rubber accumulation and PCD by subjecting the Decaisnea insignis laticiferous canals to light microscopy, TUNEL assay, and DAPI staining, as well as viability analysis, cellular ultrastructure analysis, and molecular analysis using light microscopy, scanning electron microscopy, immunofluorescence labeling, transmission electron microscopy, and transcriptome sequencing. At the cellular level, the origin of small rubber particles in the laticiferous canals had no morphological correlation with other organelles, and these particles were freely produced in the cytosol. The volume of the rubber particles increased at the sunken and expanding stage, which were identified as having the characteristics of programmed cell death (PCD); meanwhile, plenty of the rubber precursors or rubber particles were engulfed by the vacuoles, indicating a vacuole-mediated autophagy process. The accumulation of rubber particles occurred after the degeneration of protoplasts, suggesting a close association between rubber biosynthesis and PCD. The molecular analysis revealed the expression patterns of key genes involved in rubber biosynthesis. The upstream genes DiIPP, DiFPP, and DiGGPPS showed a decreasing trend during fruit ripening, while DiHRT, which is responsible for rubber particle extension, exhibited the highest expression level during the rubber particle formation. Moreover, the transcription factors related to PCD, DiLSD1, and DiLOL2 showed a negative correlation with the expression pattern of DiHRT, thus exhibiting strict rules of sequential expression during rubber biosynthesis. Additionally, the expression trends of DiXCP1 and DiCEP1, which act as proteases during PCD, were positively correlated with DiGGPPS expression. In conclusion, the findings suggest that the autophagic PCD may play a crucial role in rubber accumulation in D. insignis. Further research is still needed to fully understand the complex regulatory network underlying rubber biosynthesis in plants. [ABSTRACT FROM AUTHOR]

Natural rubber is one of the most important industrial raw materials, and its biosynthesis is still a fascinating process that is still largely unknown. In this research, we studied Decaisnea insignis, a unique rubber-producing plant that is different from other rubber-producing species due to the presence of lactiferous canals in its pericarp. The present study aims to provide novel insights into the mechanisms underlying rubber accumulation and PCD by subjecting the Decaisnea insignis laticiferous canals to light microscopy, TUNEL assay, and DAPI staining, as well as viability analysis, cellular ultrastructure analysis, and molecular analysis using light microscopy, scanning electron microscopy, immunofluorescence labeling, transmission electron microscopy, and transcriptome sequencing. At the cellular level, the origin of small rubber particles in the laticiferous canals had no morphological correlation with other organelles, and these particles were freely produced in the cytosol. The volume of the rubber particles increased at the sunken and expanding stage, which were identified as having the characteristics of programmed cell death (PCD); meanwhile, plenty of the rubber precursors or rubber particles were engulfed by the vacuoles, indicating a vacuole-mediated autophagy process. The accumulation of rubber particles occurred after the degeneration of protoplasts, suggesting a close association between rubber biosynthesis and PCD. The molecular analysis revealed the expression patterns of key genes involved in rubber biosynthesis. The upstream genes DiIPP, DiFPP, and DiGGPPS showed a decreasing trend during fruit ripening, while DiHRT, which is responsible for rubber particle extension, exhibited the highest expression level during the rubber particle formation. Moreover, the transcription factors related to PCD, DiLSD1, and DiLOL2 showed a negative correlation with the expression pattern of DiHRT, thus exhibiting strict rules of sequential expression during rubber biosynthesis. Additionally, the expression trends of DiXCP1 and DiCEP1, which act as proteases during PCD, were positively correlated with DiGGPPS expression. In conclusion, the findings suggest that the autophagic PCD may play a crucial role in rubber accumulation in D. insignis. Further research is still needed to fully understand the complex regulatory network underlying rubber biosynthesis in plants.

Introduction: Programmed cell death (PCD) is a fundamental biological process crucial for plant development. Despite recent advancements in our understanding of PCD's molecular mechanisms, the intricate orchestration of this process within plant cells remains enigmatic. To address this knowledge gap, the present study focuses on Decaisnea insignis , a plant species renowned for its unique fruit anatomy, including laticiferous canals that secrete latex. While extensive anatomical studies have elucidated the structural features of these canals,molecular insights into their developmental regulation, particularly the involvement of PCD, are lacking., Methods: In this study, we sequenced the single-cell transcriptomes at two developmental stage of Decaisnea insignis fruit using the technology known as 10x Genomics (S1, S2). Using sequencing technology combining full- length RNA sequencing and single-nucleus RNA sequencing (snRNA-seq) in combination with ultrastructural analyses, our study revealed a cellular map of Decaisnea insignis fruit at the single-cell level and identified different cell types., Results: In particular, we identified a possible PCD-mediated cluster of Decaisnea insignis fruit lactiferous canals in epidermal cells and clarified the expression patterns of DiRD21A (a hydrolase) and DiLSD1 (a transcription factor), which may be closely related to the development of laticiferous canals in Decaisnea insignis fruits., Discussion: By integrating high-resolution gene expression profiling with visual insights into cellular transformations, we sought to more precisely characterize the regulatory role of PCD during the developmental formation of lactiferous canals in Decaisnea insignis fruit., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Li, Zhao, Shi, Li, Yang, Wang and Zhou.)

Four extraction techniques including enzyme-assisted supercritical carbon dioxide extraction (ESCCO2), supercritical carbon dioxide extraction (SC-CO2), ultrasound-assisted extraction (UAE) and Soxhlet extraction (SE) were evaluated to efficiently extract the oil from Decaisnea insignis seeds in this study. Lipid profiling by GC-MS confirmed seven fatty acids and six phytosterols in all oils obtained, in which palmitoleic acid (from 475.4 ± 12.0 by SE to 604.9 ± 26.1 mg/g oil by ESC-CO2) and ß-sitosterol (from 35.6 ± 1.2 by SE to 60.9 ± 2.3 mg cholestanol equivalent/100 g oil by ESC-CO2) predominated, respectively. Furthermore, the oil yielded by ESC-CO2 displayed higher polyphenol content and stronger DPPH and ABTS radical scavenging capacities. These results suggested that D. insignis seed oil is considered an alternative source of palmitoleic acid, and ESC-CO2 could be explored as an environmentally friendly technology for extracting plant oil. [ABSTRACT FROM AUTHOR]

Background and Aims: Decaisnea insignis, known as ‘dead man's fingers’ (Lardizabalaceae), is widely distributed in China and the Himalayan foothill countries. This economically important plant, which is the only species in the genus, has not been the subject of any embryological studies aside from one brief, older paper that lacks micrographs. Data on Decaisnea are also important because its systematic position has been unstable since the genus was established in 1855. Therefore, the objectives of this study were: (a) to use modern microscopy to document early reproductive anatomical development in Decaisnea; and (b) to compare qualitatively these early embryological characters with allied taxa in a systematic context. [ABSTRACT FROM PUBLISHER]