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3 results on '"Deborah Santini"'

1. Grapevine Grafting: Scion Transcript Profiling and Defense-Related Metabolites Induced by Rootstocks

Author

Walter Chitarra, Irene Perrone, Carla G. Avanzato, Andrea Minio, Paolo Boccacci, Deborah Santini, Giovanna Gilardi, Ilenia Siciliano, Maria L. Gullino, Massimo Delledonne, Franco Mannini, and Giorgio Gambino

Subjects
Vitis, abscisic acid, stilbene compounds, transcriptome, pathogens, Plasmopara viticola, Plant culture, SB1-1110
Abstract

Rootstocks are among the main factors that influence grape development as well as fruit and wine composition. In this work, rootstock/scion interactions were studied using transcriptomic and metabolic approaches on leaves of the “Gaglioppo” variety, grafted onto 13 different rootstocks growing in the same vineyard. The whole leaf transcriptome of “Gaglioppo” grafted onto five selected rootstocks showed high variability in gene expression. In particular, significant modulation of transcripts linked to primary and secondary metabolism was observed. Interestingly, genes and metabolites involved in defense responses (e.g., stilbenes and defense genes) were strongly activated particularly in the GAG-41B combination, characterized in addition by the down-regulation of abscisic acid (ABA) metabolism. On the contrary, the leaves of “Gaglioppo” grafted onto 1103 Paulsen showed an opposite regulations of those transcripts and metabolites, together with the greater sensitivity to downy mildew in a preliminary in vitro assay. This study carried out an extensive transcriptomic analysis of rootstock effects on scion leaves, helping to unravel this complex interaction, and suggesting an interesting correlation among constitutive stilbenes, ABA compound, and disease susceptibility to a fungal pathogen.

Published
2017
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2. Modifications in chemical, physical and mechanical properties of Nebbiolo (Vitis Vinifera L.) grape berries induced by mixed virus infection

Author

Franco Mannini, Luca Giorgio Carlo Rolle, Patrizia Cascio, and Deborah Santini

Subjects
0106 biological sciences, GFLV, Cyanidin, GLRaV-1, GLRaV-3, GVA, berry-skin thickness, anthocyanin extractability, Berry, Biology, 01 natural sciences, 040501 horticulture, chemistry.chemical_compound, Yield (wine), Plant virus, Wine, Crop yield, food and beverages, 04 agricultural and veterinary sciences, Horticulture, chemistry, Anthocyanin, Composition (visual arts), 0405 other agricultural sciences, 010606 plant biology & botany
Abstract

Modifications in grape quality parameters induced by mixed infection with GFLV and GFkV, GLRaV-1 and GVA, and GLRaV-3 and GVA in three Nebbiolo clones were compared against healthy plants of the same clones in two experimental vineyards in Piemonte, northwest Italy. The aim of the study was to evaluate the effect of virus infection on the mechanical properties of the berry skin and the whole berry as assessed by texture analysis tests, and on the amount and quality of berry skin phenols. Differences were observed in grapevine vigour, yield and juice composition, depending on the viral status of the plants. The anthocyanin profile of the vines infected with GFV and GFkV and those infected with GLRaV-1 and GVA showed a lower percentage of the more stable tri-substituted malvidin-3-glucoside and a higher percentage of cyanidin and peonidin-3-glucosides. Texture analysis showed that the viruses may increase berry-skin thickness and reduce phenol extractability. These effects carry practical implications for wine quality.

Published
2011

3. A multidisciplinary study on the effects of phloem - limited viruses on the agronomical perfomance and berry quality of Vitis vinifera cv Nebbiolo

Author

Laura Cavallarin, Cristina Marzachì, Marzia Giribaldi, Franco Mannini, Maria Gabriella Giuffrida, Luca Giorgio Carlo Rolle, M. Purrotti, D. Pacifico, Deborah Santini, and Piero Caciagli

Subjects
Crops, Agricultural, Proteomics, 2D-elettroforesi, Biophysics, Titratable acid, Berry, Phloem, engineering.material, Biology, Biochemistry, Vineyard, grape quality, Yield (wine), Botany, Electrophoresis, Gel, Two-Dimensional, Vitis, Plant Diseases, Plant Proteins, mass spectrometry, Spots, Pulp (paper), Infezioni virali, food and beverages, spettrometria di massa, Horticulture, Fruit, Viruses, Shoot, engineering, Qualità delle uve, 2D electrophoresis, instrumental texture analysis, viral infection
Abstract

Viral infections are known to have a detrimental effect on grapevine yield and performance, but there is still a lack of knowledge about their effect on the quality and safety of end products. Vines of Vitis vinifera cv. Nebbiolo clone 308, affected simultaneously by Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine virus A (GVA), and Rupestris stem pitting associated virus (RSPaV), were subjected to integrated analyses of agronomical performance, grape berry characteristics, instrumental texture profile, and proteome profiling. The comparison of performance and grape quality of healthy and infected vines cultivated in a commercial vineyard revealed similar shoot fertility, number of clusters, total yield, with significant differences in titratable acidity, and resveratrol content. Also some texture parameters such as cohesiveness and resilience were altered in berries of infected plants. The proteomic analysis of skin and pulp visualized about 400 spots. The ANOVA analysis on 2D gels revealed significant differences among healthy and virus-infected grape berries for 12 pulp spots and 7 skin spots. Virus infection mainly influenced proteins involved in the response to oxidative stress in the berry skin, and proteins involved in cell structure metabolism in the pulp.

Published
2011

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