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553 results on '"Debabov, V."'

15. Optimization of the Biosynthesis of Butyric Acid from Glucose through the Inverted Fatty Acid β-Oxidation Pathway by Recombinant Escherichia coli Strains.

Author

Gulevich, A. Yu., Skorokhodova, A. Yu., and Debabov, V. G.

Subjects
BUTYRIC acid, FATTY acids, ESCHERICHIA coli, BIOSYNTHESIS, GLUCOSE, GENE expression
Abstract

The biosynthesis of butyric acid from glucose through inverted fatty acid β-oxidation by recombinant Escherichia coli strains was optimized. An increased yield of the target compound was achieved resulting from the plasmid expression of the atoB, fadB, and fadE/fabI genes in the core strain MG∆4 PL-tesB ΔyciA (MG1655 ∆ackA-pta, ∆poxB, ∆ldhA, ∆adhE, PL-SDϕ10-tesB, ∆yciA). The positive effect of enforced ATP hydrolysis on microaerobic conversion of the carbohydrate substrate to the final product by the recombinants was demonstrated. Activation of the futile cycle of pyruvate–phosphoenolpyruvate–pyruvate, due to the increased expression of the ppsA gene, ensured a marked increase in glucose consumption by the recombinants and led to an increase in the molar yield of butyric acid up to 39.5%. When the components of the H+-ATP synthase complex were uncoupled resulting from the deletion of atpFH genes, the molar yield of butyric acid from glucose demonstrated by the strain forming butyryl-CoA by the action of enoyl-ACP reductase FabI reached 46%. [ABSTRACT FROM AUTHOR]

Published
2024
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33. Analysis of the Reducing Activity of Strains of the Genus ShewanellaIsolated from Various Natural Sources Using the Example of the Biosynthesis of Silver Nanoparticles

Author

Voeikova, T. A., Zhuravliova, O. A., Babich, T. L., Malakhov, S. N., Patsaev, T. D., Vasiliev, A. L., Kozhukhova, E. I., Zhdanov, P. A., Vlasova, A. Yu., Kilochek, A. I., and Debabov, V. G.

Abstract

Abstract: For the first time, a comparative analysis of 8 strains of the genus Shewanellaisolated from various natural sources is carried out according to the level of the reducing activity of Ag+to silver-containing nanoparticles (NPs). The characteristics of biogenic NPs in terms of shape, size, crystal-lattice parameters, hydrodynamic diameter, ζ potential, and optical characteristics are obtained using scanning transmission electron microscopy, dynamic light scattering, spectrophotometry, atomic emission spectroscopy, and assessment of the biocidal activity against various types of microorganisms, including the studied strains of the genus Shewanella. It is found that the shape of the NPs is close to spherical, the sizes vary from 2 to 30 nm, the samples are polydisperse colloidal systems. Analysis of crystal lattices of the NPs confirms the presence of AgNPs, as well as Ag2O2NPs and Ag3O4NPs in some samples. The results of atomic emission spectroscopy on the quantitative analysis of the content of Ag in the nanomaterial samples are obtained, on the basis of which strains with a high ability to form a Ag-containing nanomaterial are identified. The high sensitivity of gram-positive bacteria to AgNPs is established. Different levels of resistance of 8 strains of the genus Shewanellato the action of biogenic AgNPs are demonstrated. The results indicate the metabolic diversity of strains of the genus Shewanellaisolated from various natural sources. The analysis of strains of the genus Shewanellaaccording to the level of the reducing activity is relevant for the development of an effective technology for the “green” synthesis of biogenic NPs.

Published
2024
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