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Your search keyword '"DeLaney, Alexandra A."' showing total 14 results
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14 results on '"DeLaney, Alexandra A."'

4. Genetic targeting of Card19 is linked to disrupted NINJ1 expression, impaired cell lysis, and increased susceptibility to Yersinia infection

Author

Bjanes, Elisabet, primary, Sillas, Reyna Garcia, additional, Matsuda, Rina, additional, Demarco, Benjamin, additional, Fettrelet, Timothée, additional, DeLaney, Alexandra A., additional, Kornfeld, Opher S., additional, Lee, Bettina L., additional, Rodríguez López, Eric M., additional, Grubaugh, Daniel, additional, Wynosky-Dolfi, Meghan A., additional, Philip, Naomi H., additional, Krespan, Elise, additional, Tovar, Dorothy, additional, Joannas, Leonel, additional, Beiting, Daniel P., additional, Henao-Mejia, Jorge, additional, Schaefer, Brian C., additional, Chen, Kaiwen W., additional, Broz, Petr, additional, and Brodsky, Igor E., additional

Published
2021
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5. Treatment combining RU486 and Ad5IL-12 vector attenuates the growth of experimentally formed prostate tumors and induces changes in the sentinel lymph nodes of mice

Author

Sercarz Eli E, Gauldie Jack, Graham Frank L, Halder Ramesh, Gee Jennifer, DeLaney Alexandra, Gabaglia Claudia, and Braciak Todd A

Subjects
Medicine
Abstract

Abstract Background Tumor immune responses are first generated and metastases often begin in tumor sentinel lymph nodes (TSLN). Therefore, it is important to promote tumor immunity within this microenvironment. Mifepristone (RU486) treatment can interfere with cortisol signaling that can lead to suppression of tumor immunity. Here, we assessed whether treatment with RU486 in conjunction with an intratumor injection of Ad5IL-12 vector (a recombinant adenovirus expressing IL-12) could impact the TSLN microenvironment and prostate cancer progression. Methods The human PC3, LNCaP or murine TRAMP-C1 prostate cancer cell lines were used to generate subcutaneous tumors in NOD.scid and C57BL/6 mice, respectively. Adjuvant effects of RU486 were looked for in combination therapy with intratumor injections (IT) of Ad5IL-12 vector in comparison to PBS, DL70-3 vector, DL70-3 + RU486, RU486 and Ad5IL-12 vector treatment controls. Changes in tumor growth, cell cytotoxic activity and populations of CD4+/FoxP3+ T regulatory cells (Treg) in the TSLN were evaluated. Results Treatment of human PC3 prostate xenograft or TRAMP-C1 tumors with combination Ad5IL-12 vector and RU486 produced significantly better therapeutic efficacy in comparison to controls. In addition, we found that combination therapy increased the capacity of TSLN lymphocytes to produce Granzyme B in response to tumor cell targets. Finally, combination therapy tended towards decreases of CD4+/FoxP3+ T regulatory cell populations to be found in the TSLN. Conclusion Inclusion of RU486 may serve as a useful adjuvant when combined with proinflammatory tumor killing agents by enhancement of the immune response and alteration of the TSLN microenvironment.

Published
2010
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6. Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock

Author

Mandal, Pratyusha, primary, Feng, Yanjun, additional, Lyons, John D., additional, Berger, Scott B., additional, Otani, Shunsuke, additional, DeLaney, Alexandra, additional, Tharp, Gregory K., additional, Maner-Smith, Kristal, additional, Burd, Eileen M., additional, Schaeffer, Michelle, additional, Hoffman, Sandra, additional, Capriotti, Carol, additional, Roback, Linda, additional, Young, Cedrick B., additional, Liang, Zhe, additional, Ortlund, Eric A., additional, DiPaolo, Nelson C., additional, Bosinger, Steven, additional, Bertin, John, additional, Gough, Peter J., additional, Brodsky, Igor E., additional, Coopersmith, Craig M., additional, Shayakhmetov, Dmitry M., additional, and Mocarski, Edward S., additional

Published
2018
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7. Group 1 Innate Lymphoid Cell Lineage Identity Is Determined by a cis-Regulatory Element Marked by a Long Non-coding RNA

Author

Mowel, Walter K., primary, McCright, Sam J., additional, Kotzin, Jonathan J., additional, Collet, Magalie A., additional, Uyar, Asli, additional, Chen, Xin, additional, DeLaney, Alexandra, additional, Spencer, Sean P., additional, Virtue, Anthony T., additional, Yang, EnJun, additional, Villarino, Alejandro, additional, Kurachi, Makoto, additional, Dunagin, Margaret C., additional, Pritchard, Gretchen Harms, additional, Stein, Judith, additional, Hughes, Cynthia, additional, Fonseca-Pereira, Diogo, additional, Veiga-Fernandes, Henrique, additional, Raj, Arjun, additional, Kambayashi, Taku, additional, Brodsky, Igor E., additional, O’Shea, John J., additional, Wherry, E. John, additional, Goff, Loyal A., additional, Rinn, John L., additional, Williams, Adam, additional, Flavell, Richard A., additional, and Henao-Mejia, Jorge, additional

Published
2017
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8. RIPK1-dependent apoptosis bypasses pathogen blockade of innate signaling to promote immune defense

Author

Peterson, Lance W., primary, Philip, Naomi H., additional, DeLaney, Alexandra, additional, Wynosky-Dolfi, Meghan A., additional, Asklof, Kendra, additional, Gray, Falon, additional, Choa, Ruth, additional, Bjanes, Elisabet, additional, Buza, Elisabeth L., additional, Hu, Baofeng, additional, Dillon, Christopher P., additional, Green, Douglas R., additional, Berger, Scott B., additional, Gough, Peter J., additional, Bertin, John, additional, and Brodsky, Igor E., additional

Published
2017
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9. Activity of Uncleaved Caspase-8 Controls Anti-bacterial Immune Defense and TLR-Induced Cytokine Production Independent of Cell Death

Author

Philip, Naomi H., primary, DeLaney, Alexandra, additional, Peterson, Lance W., additional, Santos-Marrero, Melanie, additional, Grier, Jennifer T., additional, Sun, Yan, additional, Wynosky-Dolfi, Meghan A., additional, Zwack, Erin E., additional, Hu, Baofeng, additional, Olsen, Tayla M., additional, Rongvaux, Anthony, additional, Pope, Scott D., additional, López, Carolina B., additional, Oberst, Andrew, additional, Beiting, Daniel P., additional, Henao-Mejia, Jorge, additional, and Brodsky, Igor E., additional

Published
2016
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13. Isothermal Detection of Mycoplasma pneumoniaeDirectly from Respiratory Clinical Specimens

Author

Petrone, Brianna L., Wolff, Bernard J., DeLaney, Alexandra A., Diaz, Maureen H., and Winchell, Jonas M.

Abstract

ABSTRACTMycoplasma pneumoniaeis a leading cause of community-acquired pneumonia (CAP) across patient populations of all ages. We have developed a loop-mediated isothermal amplification (LAMP) assay that enables rapid, low-cost detection of M. pneumoniaefrom nucleic acid extracts and directly from various respiratory specimen types. The assay implements calcein to facilitate simple visual readout of positive results in approximately 1 h, making it ideal for use in primary care facilities and resource-poor settings. The analytical sensitivity of the assay was determined to be 100 fg by testing serial dilutions of target DNA ranging from 1 ng to 1 fg per reaction, and no cross-reactivity was observed against 17 other Mycoplasmaspecies, 27 common respiratory agents, or human DNA. We demonstrated the utility of this assay by testing nucleic acid extracts (n= 252) and unextracted respiratory specimens (n= 72) collected during M. pneumoniaeoutbreaks and sporadic cases occurring in the United States from February 2010 to January 2014. The sensitivity of the LAMP assay was 88.5% tested on extracted nucleic acid and 82.1% evaluated on unextracted clinical specimens compared to a validated real-time PCR test. Further optimization and improvements to this method may lead to the availability of a rapid, cost-efficient laboratory test for M. pneumoniaedetection that is more widely available to primary care facilities, ultimately facilitating prompt detection and appropriate responses to potential M. pneumoniaeoutbreaks and clusters within the community.

Published
2015
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14. Increased BMP6 Levels in the Brains of Alzheimer's Disease Patients and APP Transgenic Mice Are Accompanied by Impaired Neurogenesis.

Author

Crews, Leslie, Adame, Anthony, Patrick, Christina, DeLaney, Alexandra, Pham, Emiley, Rockenstein, Edward, Hansen, Lawrence, and Masliah, Eliezer

Subjects
ALZHEIMER'S disease research, TRANSGENIC mice, DEVELOPMENTAL neurobiology, AGING, NEUROPLASTICITY
Abstract

During aging and in the progression of Alzheimer's disease (AD), synaptic plasticity and neuronal integrity are disturbed. In addition to the alterations in plasticity in mature neurons, the neurodegenerative process in AD has been shown to be accompanied by alterations in neurogenesis. Members of the bone morphogenetic protein (BMP) family of growth factors have been implicated as important regulators of neurogenesis and neuronal cell fate determination during development; however, their role in adult neurogenesis and in AD is less clear. We show here by qRT-PCR analysis that BMP6 mRNA levels were significantly increased in the hippocampus of human patients with AD and in APP transgenic mice compared to controls. Immunoblot and immunohistochemical analyses confirmed that BMP6 protein levels were increased in human AD brains and APP transgenic mouse brains compared to controls and accumulated around hippocampal plaques. The increased levels of BMP6 were accompanied by defects in hippocampal neurogenesis in AD patients and APP transgenic mice. In support of a role for BMP6 in defective neurogenesis in AD, we show in an in vitro model of adult neurogenesis that treatment with amyloid-β1-42 protein (Aβ) resulted in increased expression of BMP6, and that exposure to recombinant BMP6 resulted in reduced proliferation with no toxic effects. Together, these results suggest that Aβ-associated increases in BMP6 expression in AD may have deleterious effects on neurogenesis in the hippocampus, and therapeutic approaches could focus on normalization of BMP6 levels to protect against AD-related neurogenic deficits. [ABSTRACT FROM AUTHOR]

Published
2010
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