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3. Deep optical neural network by living tumour brain cells

Author

Pierangeli, D., Palmieri, V., Marcucci, G., Moriconi, C., Perini, G., De Spirito, M., Papi, M., and Conti, C.

Subjects
Physics - Medical Physics, Condensed Matter - Disordered Systems and Neural Networks, Nonlinear Sciences - Adaptation and Self-Organizing Systems, Physics - Biological Physics, Physics - Optics
Abstract

The new era of artificial intelligence demands large-scale ultrafast hardware for machine learning. Optical artificial neural networks process classical and quantum information at the speed of light, and are compatible with silicon technology, but lack scalability and need expensive manufacturing of many computational layers. New paradigms, as reservoir computing and the extreme learning machine, suggest that disordered and biological materials may realize artificial neural networks with thousands of computational nodes trained only at the input and at the readout. Here we employ biological complex systems, i.e., living three-dimensional tumour brain models, and demonstrate a random neural network (RNN) trained to detect tumour morphodynamics via image transmission. The RNN, with the tumour spheroid as a three-dimensional deep computational reservoir, performs programmed optical functions and detects cancer morphodynamics from laser-induced hyperthermia inaccessible by optical imaging. Moreover, the RNN quantifies the effect of chemotherapy inhibiting tumour growth. We realize a non-invasive smart probe for cytotoxicity assay, which is at least one order of magnitude more sensitive with respect to conventional imaging. Our random and hybrid photonic/living system is a novel artificial machine for computing and for the real-time investigation of tumour dynamics., Comment: 14 pages, 4 figures

Published
2018

5. Multifunctional scaffolds for biomedical applications: Crafting versatile solutions with polycaprolactone enriched by graphene oxide

Author

Friggeri, G., primary, Moretti, I., additional, Amato, F., additional, Marrani, A. G., additional, Sciandra, F., additional, Colombarolli, S. G., additional, Vitali, A., additional, Viscuso, S., additional, Augello, A., additional, Cui, L., additional, Perini, G., additional, De Spirito, M., additional, Papi, M., additional, and Palmieri, V., additional

Published
2024
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6. Advancements in Mid-Infrared spectroscopy of extracellular vesicles

Author

Di Santo, R., Niccolini, Benedetta, Romano, S., Vaccaro, Maria, Di Giacinto, Flavio, De Spirito, Marco, Ciasca, Gabriele, Niccolini B., Vaccaro M., Di Giacinto F. (ORCID:0000-0002-6726-7768), De Spirito M. (ORCID:0000-0003-4260-5107), Ciasca G. (ORCID:0000-0002-3694-8229), Di Santo, R., Niccolini, Benedetta, Romano, S., Vaccaro, Maria, Di Giacinto, Flavio, De Spirito, Marco, Ciasca, Gabriele, Niccolini B., Vaccaro M., Di Giacinto F. (ORCID:0000-0002-6726-7768), De Spirito M. (ORCID:0000-0003-4260-5107), and Ciasca G. (ORCID:0000-0002-3694-8229)

Abstract

Extracellular vesicles (EVs) are lipid vesicles secreted by all cells into the extracellular space and act as nanosized biological messengers among cells. They carry a specific molecular cargo, composed of lipids, proteins, nucleic acids, and carbohydrates, which reflects the state of their parent cells. Due to their remarkable structural and compositional heterogeneity, characterizing EVs, particularly from a biochemical perspective, presents complex challenges. In this context, mid-infrared (IR) spectroscopy is emerging as a valuable tool, providing researchers with a comprehensive and label-free spectral fingerprint of EVs in terms of their specific molecular content. This review aims to provide an up-to-date critical overview of the major advancements in mid-IR spectroscopy of extracellular vesicles, encompassing both fundamental and applied research achievements. We also systematically emphasize the new possibilities offered by the integration of emerging cutting-edge IR technologies, such as tip-enhanced and surface-enhanced spectroscopy approaches, along with the growing use of machine learning for data analysis and spectral interpretation. Additionally, to assist researchers in navigating this intricate subject, our manuscript includes a wide and detailed collection of the spectral peaks that have been assigned to EV molecular constituents up to now in the literature.

Published
2024

7. Multifunctional scaffolds for biomedical applications: Crafting versatile solutions with polycaprolactone enriched by graphene oxide

Author

Friggeri, G., Moretti, Irene, Amato, F., Marrani, A. G., Sciandra, Francesca, Colombarolli, S. G., Vitali, A., Viscuso, S., Augello, A., Cui, Lishan, Perini, Giordano, De Spirito, Marco, Papi, Massimiliano, Palmieri, V., Moretti I., Sciandra F., Cui L., Perini G. (ORCID:0000-0001-9452-8479), De Spirito M. (ORCID:0000-0003-4260-5107), Papi M. (ORCID:0000-0002-0029-1309), Friggeri, G., Moretti, Irene, Amato, F., Marrani, A. G., Sciandra, Francesca, Colombarolli, S. G., Vitali, A., Viscuso, S., Augello, A., Cui, Lishan, Perini, Giordano, De Spirito, Marco, Papi, Massimiliano, Palmieri, V., Moretti I., Sciandra F., Cui L., Perini G. (ORCID:0000-0001-9452-8479), De Spirito M. (ORCID:0000-0003-4260-5107), and Papi M. (ORCID:0000-0002-0029-1309)

Abstract

The pressing need for multifunctional materials in medical settings encompasses a wide array of scenarios, necessitating specific tissue functionalities. A critical challenge is the occurrence of biofouling, particularly by contamination in surgical environments, a common cause of scaffolds impairment. Beyond the imperative to avoid infections, it is also essential to integrate scaffolds with living cells to allow for tissue regeneration, mediated by cell attachment. Here, we focus on the development of a versatile material for medical applications, driven by the diverse time-definite events after scaffold implantation. We investigate the potential of incorporating graphene oxide (GO) into polycaprolactone (PCL) and create a composite for 3D printing a scaffold with time-controlled antibacterial and anti-adhesive growth properties. Indeed, the as-produced PCL-GO scaffold displays a local hydrophobic effect, which is translated into a limitation of biological entities-attachment, including a diminished adhesion of bacteriophages and a reduction of E. coli and S. aureus adhesion of ∼81% and ∼69%, respectively. Moreover, the ability to 3D print PCL-GO scaffolds with different heights enables control over cell distribution and attachment, a feature that can be also exploited for cellular confinement, i.e., for microfluidics or wound healing applications. With time, the surface wettability increases, and the scaffold can be populated by cells. Finally, the presence of GO allows for the use of infrared light for the sterilization of scaffolds and the disruption of any bacteria cell that might adhere to the more hydrophilic surface. Overall, our results showcase the potential of PCL-GO as a versatile material for medical applications.

Published
2024

8. Slow and steady wins the race: Fractionated near-infrared treatment empowered by graphene-enhanced 3D scaffolds for precision oncology

Author

Perini, Giordano, Palmieri, V., Papait, Andrea, Augello, A., Fioretti, D., Iurescia, S., Rinaldi, M., Vertua, E., Silini, Antonietta Rosa, Torelli, R., Carlino, Anastasia, Musarra, T., Sanguinetti, Maurizio, Parolini, Ornella, De Spirito, Marco, Papi, Massimiliano, Perini G. (ORCID:0000-0001-9452-8479), Papait A. (ORCID:0000-0003-1229-9671), Silini A., Carlino A., Sanguinetti M. (ORCID:0000-0002-9780-7059), Parolini O. (ORCID:0000-0002-5211-6430), De Spirito M. (ORCID:0000-0003-4260-5107), Papi M. (ORCID:0000-0002-0029-1309), Perini, Giordano, Palmieri, V., Papait, Andrea, Augello, A., Fioretti, D., Iurescia, S., Rinaldi, M., Vertua, E., Silini, Antonietta Rosa, Torelli, R., Carlino, Anastasia, Musarra, T., Sanguinetti, Maurizio, Parolini, Ornella, De Spirito, Marco, Papi, Massimiliano, Perini G. (ORCID:0000-0001-9452-8479), Papait A. (ORCID:0000-0003-1229-9671), Silini A., Carlino A., Sanguinetti M. (ORCID:0000-0002-9780-7059), Parolini O. (ORCID:0000-0002-5211-6430), De Spirito M. (ORCID:0000-0003-4260-5107), and Papi M. (ORCID:0000-0002-0029-1309)

Abstract

Surgically addressing tumors poses a challenge, requiring a tailored, multidisciplinary approach for each patient based on the unique aspects of their case. Innovative therapeutic regimens combined to reliable reconstructive methods can contribute to an extended patient's life expectancy. This study presents a detailed comparative investigation of near-infrared therapy protocols, examining the impact of non-fractionated and fractionated irradiation regimens on cancer treatment. The therapy is based on the implantation of graphene oxide/poly(lactic-co-glycolic acid) three-dimensional printed scaffolds, exploring their versatile applications in oncology by the examination of pro-inflammatory cytokine secretion, immune response, and in vitro and in vivo tumor therapy. The investigation into cell death patterns (apoptosis vs necrosis) underlines the pivotal role of protocol selection underscores the critical influence of treatment duration on cell fate, establishing a crucial parameter in therapeutic decision-making. In vivo experiments corroborated the profound impact of protocol selection on tumor response. The fractionated regimen emerged as the standout performer, achieving a substantial reduction in tumor size over time, surpassing the efficacy of the non-fractionated approach. Additionally, the fractionated regimen exhibited efficacy also in targeting tumors in proximity but not in direct contact to the scaffolds. Our results address a critical gap in current research, highlighting the absence of a standardized protocol for optimizing the outcome of photodynamic therapy. The findings underscore the importance of personalized treatment strategies in achieving optimal therapeutic efficacy for precision cancer therapy.

Published
2024

9. IRIDE White Book, An Interdisciplinary Research Infrastructure based on Dual Electron linacs&lasers

Author

Alesini, D., Alessandroni, M., Anania, M. P., Andreas, S., Angelone, M., Arcovito, A., Arnesano, F., Artioli, M., Avaldi, L., Babusci, D., Bacci, A., Balerna, A., Bartalucci, S., Bedogni, R., Bellaveglia, M., Bencivenga, F., Benfatto, M., Biedron, S., Bocci, V., Bolognesi, M., Bolognesi, P., Boni, R., Bonifacio, R., Boscolo, M., Boscherini, F., Bossi, F., Broggi, F., Buonomo, B., Calo', V., Catone, D., Capogni, M., Capone, M., Castellano, M., Castoldi, A., Catani, L., Cavoto, G., Cherubini, N., Chirico, G., Cestelli-Guidi, M., Chiadroni, E., Chiarella, V., Cianchi, A., Cianci, M., Cimino, R., Ciocci, F., Clozza, A., Collini, M., Colo', G., Compagno, A., Contini, G., Coreno, M., Cucini, R., Curceanu, C., Dabagov, S., Dainese, E., Davoli, I., Dattoli, G., De Caro, L., De Felice, P., Della Longa, S., Monache, G. Delle, De Spirito, M., Di Cicco, A., Di Donato, C., Di Gioacchino, D., Di Giovenale, D., Di Palma, E., Di Pirro, G., Dodaro, A., Doria, A., Dosselli, U., Drago, A., Escribano, R., Esposito, A., Faccini, R., Ferrari, A., Ferrario, M., Filabozzi, A., Filippetto, D., Fiori, F., Frasciello, O., Fulgentini, L., Gallerano, G. P., Gallo, A., Gambaccini, M., Gatti, C., Gatti, G., Gauzzi, P., Ghigo, A., Ghiringhelli, G., Giannessi, L., Giardina, G., Giannini, C., Giorgianni, F., Giovenale, E., Gizzi, L., Guaraldo, C., Guazzoni, C., Gunnella, R., Hatada, K., Ivashyn, S., Jegerlehner, F., Keeffe, P. O., Kluge, W., Kupsc, A., Iannone, M., Labate, L., Sandri, P. Levi, Lombardi, V., Londrillo, P., Loreti, S., Losacco, M., Lupi, S., Macchi, A., Magazu', S., Mandaglio, G., Marcelli, A., Margutti, G., Mariani, C., Mariani, P., Marzo, G., Masciovecchio, C., Masjuan, P., Mattioli, M., Mazzitelli, G., Merenkov, N. P., Michelato, P., Migliardo, F., Migliorati, M., Milardi, C., Milotti, E., Milton, S., Minicozzi, V., Mobilio, S., Morante, S., Moricciani, D., Mostacci, A., Muccifora, V., Murtas, F., Musumeci, P., Nguyen, F., Orecchini, A., Organtini, G., Ottaviani, P. L., Pace, E., Paci, M., Pagani, C., Pagnutti, S., Palmieri, V., Palumbo, L., Panaccione, G. C., Papadopoulos, C. F., Papi, M., Passera, M., Pasquini, L., Pedio, M., Perrone, A., Petralia, A., Petrillo, C., Petrillo, V., Pillon, M., Pierini, P., Pietropaolo, A., Polosa, A. D., Pompili, R., Portoles, J., Prosperi, T., Quaresima, C., Quintieri, L., Rau, J. V., Reconditi, M., Ricci, A., Ricci, R., Ricciardi, G., Ripiccini, E., Romeo, S., Ronsivalle, C., Rosato, N., Rosenzweig, J. B., Rossi, G., Rossi, A. A., Rossi, A. R., Rossi, F., Russo, D., Sabatucci, A., Sabia, E., Sacchetti, F., Salducco, S., Sannibale, F., Sarri, G., Scopigno, T., Serafini, L., Sertore, D., Shekhovtsova, O., Spassovsky, I., Spadaro, T., Spataro, B., Spinozzi, F., Stecchi, A., Stellato, F., Surrenti, V., Tenore, A., Torre, A., Trentadue, L., Turchini, S., Vaccarezza, C., Vacchi, A., Valente, P., Venanzoni, G., Vescovi, S., Villa, F., Zanotti, G., Zema, N., and Zobov, M.

Subjects
Physics - Instrumentation and Detectors, High Energy Physics - Experiment, Physics - Accelerator Physics
Abstract

This report describes the scientific aims and potentials as well as the preliminary technical design of IRIDE, an innovative tool for multi-disciplinary investigations in a wide field of scientific, technological and industrial applications. IRIDE will be a high intensity 'particle factory', based on a combination of a high duty cycle radio-frequency superconducting electron linac and of high energy lasers. Conceived to provide unique research possibilities for particle physics, for condensed matter physics, chemistry and material science, for structural biology and industrial applications, IRIDE will open completely new research possibilities and advance our knowledge in many branches of science and technology. IRIDE will contribute to open new avenues of discoveries and to address most important riddles: What does matter consist of? What is the structure of proteins that have a fundamental role in life processes? What can we learn from protein structure to improve the treatment of diseases and to design more efficient drugs? But also how does an electronic chip behave under the effect of radiations? How can the heat flow in a large heat exchanger be optimized? The scientific potential of IRIDE is far reaching and justifies the construction of such a large facility in Italy in synergy with the national research institutes and companies and in the framework of the European and international research. It will impact also on R&D work for ILC, FEL, and will be complementarity to other large scale accelerator projects. IRIDE is also intended to be realized in subsequent stages of development depending on the assigned priorities., Comment: 270 pages

Published
2013

11. IRIDE: Interdisciplinary research infrastructure based on dual electron linacs and lasers

Author

Ferrario, M, Alesini, D, Alessandroni, M, Anania, MP, Andreas, S, Angelone, M, Arcovito, A, Arnesano, F, Artioli, M, Avaldi, L, Babusci, D, Bacci, A, Balerna, A, Bartalucci, S, Bedogni, R, Bellaveglia, M, Bencivenga, F, Benfatto, M, Biedron, S, Bocci, V, Bolognesi, M, Bolognesi, P, Boni, R, Bonifacio, R, Boscherini, F, Boscolo, M, Bossi, F, Broggi, F, Buonomo, B, Calo, V, Catone, D, Capogni, M, Capone, M, Cassou, K, Castellano, M, Castoldi, A, Catani, L, Cavoto, G, Cherubini, N, Chirico, G, Cestelli-Guidi, M, Chiadroni, E, Chiarella, V, Cianchi, A, Cianci, M, Cimino, R, Ciocci, F, Clozza, A, Collini, M, Colo, G, Compagno, A, Contini, G, Coreno, M, Cucini, R, Curceanu, C, Curciarello, F, Dabagov, S, Dainese, E, Davoli, I, Dattoli, G, De Caro, L, De Felice, P, De Leo, V, Dell Agnello, S, Della Longa, S, Delle Monache, G, De Spirito, M, Di Cicco, A, Di Donato, C, Di Gioacchino, D, Di Giovenale, D, Di Palma, E, Di Pirro, G, Dodaro, A, Doria, A, Dosselli, U, Drago, A, Dupraz, K, Escribano, R, Esposito, A, Faccini, R, Ferrari, A, Filabozzi, A, Filippetto, D, Fiori, F, Frasciello, O, Fulgentini, L, Gallerano, GP, Gallo, A, Gambaccini, M, Gatti, C, Gatti, G, Gauzzi, P, Ghigo, A, Ghiringhelli, G, Giannessi, L, Giardina, G, Giannini, C, Giorgianni, F, and Giovenale, E

Subjects
SC Linac, FEL, Particle physics, Neutron source, Compton source, Advanced accelerators concepts, physics.ins-det, hep-ex, physics.acc-ph, Nuclear & Particles Physics, Atomic, Molecular, Nuclear, Particle and Plasma Physics, Other Physical Sciences, Astronomical and Space Sciences, Atomic, Molecular, Nuclear, Particle and Plasma Physics
Abstract

This paper describes the scientific aims and potentials as well as the preliminary technical design of IRIDE, an innovative tool for multi-disciplinary investigations in a wide field of scientific, technological and industrial applications. IRIDE will be a high intensity "particles factory", based on a combination of high duty cycle radio-frequency superconducting electron linacs and of high energy lasers. Conceived to provide unique research possibilities for particle physics, for condensed matter physics, chemistry and material science, for structural biology and industrial applications, IRIDE will open completely new research possibilities and advance our knowledge in many branches of science and technology. IRIDE is also supposed to be realized in subsequent stages of development depending on the assigned priorities. © 2013 Elsevier B.V.

Published
2014

24. Graphene–Curcumin Coatings Resistant to SARS-CoV-2 and Mycobacteria for the Production of Personal Protective Equipment

Author

De Maio, Flavio, Santarelli, Giulia, Palmieri, V., Perini, Giordano, Salustri, Alessandro, Palucci, Ivana, Delli Carpini, Giovanni, Augello, A., Sanguinetti, Maurizio, De Spirito, Marco, Sali, M., Delogu, Giovanni, Papi, Massimiliano, De Maio F., Santarelli G., Perini G. (ORCID:0000-0001-9452-8479), Salustri A., Palucci I., Delli Carpini G., Sanguinetti M. (ORCID:0000-0002-9780-7059), De Spirito M. (ORCID:0000-0003-4260-5107), Delogu G. (ORCID:0000-0003-0182-8267), Papi M. (ORCID:0000-0002-0029-1309), De Maio, Flavio, Santarelli, Giulia, Palmieri, V., Perini, Giordano, Salustri, Alessandro, Palucci, Ivana, Delli Carpini, Giovanni, Augello, A., Sanguinetti, Maurizio, De Spirito, Marco, Sali, M., Delogu, Giovanni, Papi, Massimiliano, De Maio F., Santarelli G., Perini G. (ORCID:0000-0001-9452-8479), Salustri A., Palucci I., Delli Carpini G., Sanguinetti M. (ORCID:0000-0002-9780-7059), De Spirito M. (ORCID:0000-0003-4260-5107), Delogu G. (ORCID:0000-0003-0182-8267), and Papi M. (ORCID:0000-0002-0029-1309)

Abstract

Respiratory tract infections represent the main cause of death from infectious diseases worldwide. SARS-CoV-2 infection (i.e. COVID-19) added to the existing global burden of respiratory tract infections, including tuberculosis. Among nanomaterials for fabric functionalization, graphene, in combination with hydrophobic molecules such as phytochemicals, represents a promising low-cost alternative to antibiotics. In this work, we used graphene and curcumin to create fabric coatings on cotton and polyester for the production of personal protective equipment resistant to infective agents. These coatings ensure the trapping of microorganisms via interaction with SARS-CoV-2 or mycobacteria surface and inhibit microbial infections.

Published
2023

25. Carboxylated graphene quantum dots-mediated photothermal therapy enhances drug-membrane permeability, ROS production, and the immune system recruitment on 3D glioblastoma models

Author

Perini, Giordano, Palmieri, Valentina, Friggeri, G, Augello, A, De Spirito, Marco, Papi, Massimiliano, Perini, G (ORCID:0000-0001-9452-8479), Palmieri, V, De Spirito, M (ORCID:0000-0003-4260-5107), Papi, M (ORCID:0000-0002-0029-1309), Perini, Giordano, Palmieri, Valentina, Friggeri, G, Augello, A, De Spirito, Marco, Papi, Massimiliano, Perini, G (ORCID:0000-0001-9452-8479), Palmieri, V, De Spirito, M (ORCID:0000-0003-4260-5107), and Papi, M (ORCID:0000-0002-0029-1309)

Abstract

Graphene quantum dots (GQDs) are biocompatible nanoparticles employed in biomedical field, thanks to their size and photophysical properties. GQDs have shown the capability to cross biological barriers, including the blood-brain barrier, which makes them promising agents for brain diseases therapy. It has been shown that surface-functionalized GQDs enhance membrane fluidity and intracellular uptake, exerting a synergistic effect with antitumor drugs at subtherapeutic doses. Here, we tested GQDs effects in combination with chemotherapeutic agents doxorubicin and temozolomide, on a complex 3D spheroid model of glioblastoma. We observed that the capability of GQDs to absorb and convert near-infrared light into heat is a key factor in membrane permeability enhancement on 3D model. This non-invasive therapeutic strategy named photothermal therapy (PTT), combined to chemotherapy at subtherapeutic doses, significantly increased the effect of antitumor drugs by reducing tumor growth and viability. Furthermore, the increase in membrane permeability due to GQDs-mediated PTT enhanced the release of reactive oxygen species with strong migration of the immune system towards irradiated cancer spheroids. Our data indicate that the increase in membrane permeability can enhance the efficacy of antitumor drugs at subtherapeutic doses against glioblastoma, reducing side effects, and directing immune response, ultimately improving quality of life for patients.

Published
2023

26. Graphene oxide-mediated copper reduction allows comparative evaluation of oxygenated reactive residues exposure on the materials surface in a simple one-step method

Author

Palmieri, V., Amato, F., Marrani, A. G., Friggeri, G., Perini, Giordano, Augello, A., De Spirito, Marco, Papi, Massimiliano, Perini G. (ORCID:0000-0001-9452-8479), De Spirito M. (ORCID:0000-0003-4260-5107), Papi M. (ORCID:0000-0002-0029-1309), Palmieri, V., Amato, F., Marrani, A. G., Friggeri, G., Perini, Giordano, Augello, A., De Spirito, Marco, Papi, Massimiliano, Perini G. (ORCID:0000-0001-9452-8479), De Spirito M. (ORCID:0000-0003-4260-5107), and Papi M. (ORCID:0000-0002-0029-1309)

Abstract

Graphene Oxide (GO) is the oxidized form of graphene rich in surface groups comprising carbonyl, carboxyl, hydroxyl, and epoxy residues. The solubility in water makes GO an ideal material in the biomedical field though the plethora of synthesis methods available can modify the balance of oxygen groups and, consequently, the effects on eukaryotic and prokaryotic cells. For this reason, GO materials are always characterized with spectroscopic methods such as X-ray photoelectron and Fourier-transform Infrared spectroscopy. However, these techniques have some limitations, being disruptive and not clearly indicating oxygen functionalities available to react with polymers or biological media. In this work, we exploit GO reactivity with copper ions to develop a colorimetric method for the facile evaluation of surface oxidation degree and accessibility in polymeric samples. In the presence of GO, Cu2+ is reduced to Cu1+ and can react with bicinchoninic acid and induce light absorption at 562 nm. We observed that this reaction is dependent both on concentration and oxidation degree, and can be used to estimate GO exposure in thick composite samples. This technique will be fundamental in the future for scaffold characterization in tissue engineering and all the surface science studies analyzing GO-related materials' interactions with biological entities.

Published
2023

27. Automatic Detection of Aerobic Threshold through Recurrence Quantification Analysis of Heart Rate Time Series

Author

Zimatore, G., Serantoni, Cassandra, Gallotta, M. C., Guidetti, L., Maulucci, Giuseppe, De Spirito, Marco, Serantoni C., Maulucci G. (ORCID:0000-0002-2154-319X), De Spirito M. (ORCID:0000-0003-4260-5107), Zimatore, G., Serantoni, Cassandra, Gallotta, M. C., Guidetti, L., Maulucci, Giuseppe, De Spirito, Marco, Serantoni C., Maulucci G. (ORCID:0000-0002-2154-319X), and De Spirito M. (ORCID:0000-0003-4260-5107)

Abstract

During exercise with increasing intensity, the human body transforms energy with mechanisms dependent upon actual requirements. Three phases of the body’s energy utilization are recognized, characterized by different metabolic processes, and separated by two threshold points, called aerobic (AerT) and anaerobic threshold (AnT). These thresholds occur at determined values of exercise intensity(workload) and can change among individuals. They are considered indicators of exercise capacities and are useful in the personalization of physical activity plans. They are usually detected by ventilatory or metabolic variables and require expensive equipment and invasive measurements. Recently, particular attention has focused on AerT, which is a parameter especially useful in the overweight and obese population to determine the best amount of exercise intensity for weight loss and increasing physical fitness. The aim of study is to propose a new procedure to automatically identify AerT using the analysis of recurrences (RQA) relying only on Heart rate time series, acquired from a cohort of young athletes during a sub-maximal incremental exercise test (Cardiopulmonary Exercise Test, CPET) on a cycle ergometer. We found that the minima of determinism, an RQA feature calculated from the Recurrence Quantification by Epochs (RQE) approach, identify the time points where generic metabolic transitions occur. Among these transitions, a criterion based on the maximum convexity of the determinism minima allows to detect the first metabolic threshold. The ordinary least products regression analysis shows that values of the oxygen consumption VO2, heart rate (HR), and Workload correspondent to the AerT estimated by RQA are strongly correlated with the one estimated by CPET (r > 0.64). Mean percentage differences are <2% for both HR and VO2 and <11% for Workload. The Technical Error for HR at AerT is <8%; intraclass correlation coefficients values are moderate (≥0.66) for all

Published
2023

28. Evaluation of the Chewing Pattern through an Electromyographic Device

Author

Riente, Alessia, Abeltino, Alessio, Serantoni, Cassandra, Bianchetti, Giada, De Spirito, Marco, Capezzone, S., Esposito, R., Maulucci, Giuseppe, Riente A., Abeltino A., Serantoni C., Bianchetti G., De Spirito M. (ORCID:0000-0003-4260-5107), Maulucci G. (ORCID:0000-0002-2154-319X), Riente, Alessia, Abeltino, Alessio, Serantoni, Cassandra, Bianchetti, Giada, De Spirito, Marco, Capezzone, S., Esposito, R., Maulucci, Giuseppe, Riente A., Abeltino A., Serantoni C., Bianchetti G., De Spirito M. (ORCID:0000-0003-4260-5107), and Maulucci G. (ORCID:0000-0002-2154-319X)

Abstract

Chewing is essential in regulating metabolism and initiating digestion. Various methods have been used to examine chewing, including analyzing chewing sounds and using piezoelectric sensors to detect muscle contractions. However, these methods struggle to distinguish chewing from other movements. Electromyography (EMG) has proven to be an accurate solution, although it requires sensors attached to the skin. Existing EMG devices focus on detecting the act of chewing or classifying foods and do not provide self-awareness of chewing habits. We developed a non-invasive device that evaluates a personalized chewing style by analyzing various aspects, like chewing time, cycle time, work rate, number of chews and work. It was tested in a case study comparing the chewing pattern of smokers and non-smokers, as smoking can alter chewing habits. Previous studies have shown that smokers exhibit reduced chewing speed, but other aspects of chewing were overlooked. The goal of this study is to present the device and provide additional insights into the effects of smoking on chewing patterns by considering multiple chewing features. Statistical analysis revealed significant differences, as non-smokers had more chews and higher work values, indicating more efficient chewing. The device provides valuable insights into personalized chewing profiles and could modify unhealthy chewing habits.

Published
2023

29. Putting the Personalized Metabolic Avatar into Production: A Comparison between Deep-Learning and Statistical Models for Weight Prediction

Author

Abeltino, Alessio, Bianchetti, Giada, Serantoni, Cassandra, Riente, Alessia, De Spirito, Marco, Maulucci, Giuseppe, Abeltino A., Bianchetti G., Serantoni C., Riente A., De Spirito M. (ORCID:0000-0003-4260-5107), Maulucci G. (ORCID:0000-0002-2154-319X), Abeltino, Alessio, Bianchetti, Giada, Serantoni, Cassandra, Riente, Alessia, De Spirito, Marco, Maulucci, Giuseppe, Abeltino A., Bianchetti G., Serantoni C., Riente A., De Spirito M. (ORCID:0000-0003-4260-5107), and Maulucci G. (ORCID:0000-0002-2154-319X)

Abstract

Nutrition is a cross-cutting sector in medicine, with a huge impact on health, from cardiovascular disease to cancer. Employment of digital medicine in nutrition relies on digital twins: digital replicas of human physiology representing an emergent solution for prevention and treatment of many diseases. In this context, we have already developed a data-driven model of metabolism, called a "Personalized Metabolic Avatar" (PMA), using gated recurrent unit (GRU) neural networks for weight forecasting. However, putting a digital twin into production to make it available for users is a difficult task that as important as model building. Among the principal issues, changes to data sources, models and hyperparameters introduce room for error and overfitting and can lead to abrupt variations in computational time. In this study, we selected the best strategy for deployment in terms of predictive performance and computational time. Several models, such as the Transformer model, recursive neural networks (GRUs and long short-term memory networks) and the statistical SARIMAX model were tested on ten users. PMAs based on GRUs and LSTM showed optimal and stable predictive performances, with the lowest root mean squared errors (0.38 ± 0.16-0.39 ± 0.18) and acceptable computational times of the retraining phase (12.7 ± 1.42 s-13.5 ± 3.60 s) for a production environment. While the Transformer model did not bring a substantial improvement over RNNs in term of predictive performance, it increased the computational time for both forecasting and retraining by 40%. The SARIMAX model showed the worst performance in term of predictive performance, though it had the best computational time. For all the models considered, the extent of the data source was a negligible factor, and a threshold was established for the number of time points needed for a successful prediction.

Published
2023

30. Nanotechnology in the COVID-19 era: Carbon-based nanomaterials as a promising solution

Author

Papi, Massimiliano, De Spirito, Marco, Palmieri, V., Papi M. (ORCID:0000-0002-0029-1309), De Spirito M. (ORCID:0000-0003-4260-5107), Papi, Massimiliano, De Spirito, Marco, Palmieri, V., Papi M. (ORCID:0000-0002-0029-1309), and De Spirito M. (ORCID:0000-0003-4260-5107)

Abstract

The Coronavirus Disease 2019 (COVID-19) pandemic has led to collaboration between nanotechnology scientists, industry stakeholders, and clinicians to develop solutions for diagnostics, prevention, and treatment of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infections. Nanomaterials, including carbon-based materials (CBM) such as graphene and carbon nanotubes, have been studied for their potential in viral research. CBM unique effects on microorganisms, immune interaction, and sensitivity in diagnostics have made them a promising subject of SARS-CoV-2 research. This review discusses the interaction of CBM with SARS-CoV-2 and their applicability, including CBM physical and chemical properties, the known interactions between CBM and viral components, and the proposed prevention, treatment, and diagnostics uses.

Published
2023

31. Near-infrared controlled release of mesenchymal stem cells secretome from bioprinted graphenebased microbeads for nerve regeneration

Author

Perini, Giordano, Palmieri, V., D'Ascenzo, Marcello, Colussi, Claudia, Grassi, Claudio, Friggeri, G., Augello, A., Cui, Lishan, Papi, Massimiliano, De Spirito, Marco, Perini G. (ORCID:0000-0001-9452-8479), D'Ascenzo M. (ORCID:0000-0003-0073-412X), Colussi C., Grassi C. (ORCID:0000-0001-7253-1685), Cui L., Papi M. (ORCID:0000-0002-0029-1309), de Spirito M. (ORCID:0000-0003-4260-5107), Perini, Giordano, Palmieri, V., D'Ascenzo, Marcello, Colussi, Claudia, Grassi, Claudio, Friggeri, G., Augello, A., Cui, Lishan, Papi, Massimiliano, De Spirito, Marco, Perini G. (ORCID:0000-0001-9452-8479), D'Ascenzo M. (ORCID:0000-0003-0073-412X), Colussi C., Grassi C. (ORCID:0000-0001-7253-1685), Cui L., Papi M. (ORCID:0000-0002-0029-1309), and de Spirito M. (ORCID:0000-0003-4260-5107)

Abstract

Nerve damage is a prevalent and debilitating condition with limited treatment options. Recent years have seen an increased incidence of neural damage due to factors such as aging populations and traumatic brain injuries. Addressing the urgent need for effective therapies, this study explores the controlled delivery of mesenchymal stem cells (MSCs) secretome, a complex mixture of bioactive factors, which is currently being investigated for its potential in nerve regeneration. The secretome offers significant advantages over stem cells themselves, as it can be more easily characterized and controlled, enabling precise regulation of therapeutic interventions. However, the challenge lies in delivering the secretome specifically to the target anatomical region. To overcome this limitation, we propose a novel approach utilizing near-infrared (NIR) radiation-responsive bioprinted alginate-graphene oxide (AGO) microbeads. Graphene oxide (GO) is a highly biocompatible material with unique properties, including NIR responsiveness, enabling controlled release of therapeutic agents upon NIR exposure. We hypothesized that AGO microbeads could encapsulate MSCs secretome and release it in a controlled manner using NIR radiation. To investigate our hypothesis, controlled damage was induced to hippocampal neurons, and MSCs secretome was encapsulated within AGO microbeads. Subsequently, NIR radiation was applied to trigger the release of the secretome. We compared the efficacy of MSCs secretome with that of astrocytes, which also possess nerve growth and proliferation-promoting capabilities. Our findings demonstrated that the controlled release of MSCs secretome from AGO microbeads through non-invasive NIR radiation significantly promoted the proliferation and regeneration of neurons following nerve injury. AGO microbeads offer multiple advantages over conventional delivery methods, including precise control over the timing, location, and dosage of therapeutic agents. Furthermore, th

Published
2023

32. SPTBN1 Mediates the Cytoplasmic Constraint of PTTG1, Impairing Its Oncogenic Activity in Human Seminoma

Author

Teveroni, Emanuela, Di Nicuolo, F., Vergani, Edoardo, Oliva, A., Vodola, Emanuele Pierpaolo, Bianchetti, Giada, Maulucci, Giuseppe, De Spirito, Marco, Cenci, Tonia, Pierconti, Francesco, Gulino, Gaetano, Iavarone, Federica, Urbani, Andrea, Milardi, Domenico, Pontecorvi, Alfredo, Mancini, F., Teveroni E., Vergani E., Vodola E. P., Bianchetti G., Maulucci G. (ORCID:0000-0002-2154-319X), De Spirito M. (ORCID:0000-0003-4260-5107), Cenci T., Pierconti F. (ORCID:0000-0003-0951-4131), Gulino G., Iavarone F. (ORCID:0000-0002-2074-5531), Urbani A. (ORCID:0000-0001-9168-3174), Milardi D., Pontecorvi A. (ORCID:0000-0003-0570-6865), Teveroni, Emanuela, Di Nicuolo, F., Vergani, Edoardo, Oliva, A., Vodola, Emanuele Pierpaolo, Bianchetti, Giada, Maulucci, Giuseppe, De Spirito, Marco, Cenci, Tonia, Pierconti, Francesco, Gulino, Gaetano, Iavarone, Federica, Urbani, Andrea, Milardi, Domenico, Pontecorvi, Alfredo, Mancini, F., Teveroni E., Vergani E., Vodola E. P., Bianchetti G., Maulucci G. (ORCID:0000-0002-2154-319X), De Spirito M. (ORCID:0000-0003-4260-5107), Cenci T., Pierconti F. (ORCID:0000-0003-0951-4131), Gulino G., Iavarone F. (ORCID:0000-0002-2074-5531), Urbani A. (ORCID:0000-0001-9168-3174), Milardi D., and Pontecorvi A. (ORCID:0000-0003-0570-6865)

Abstract

Seminoma is the most common testicular cancer. Pituitary tumor-transforming gene 1 (PTTG1) is a securin showing oncogenic activity in several tumors. We previously demonstrated that nuclear PTTG1 promotes seminoma tumor invasion through its transcriptional activity on matrix metalloproteinase 2 (MMP-2) and E-cadherin (CDH1). We wondered if specific interactors could affect its subcellular distribution. To this aim, we investigated the PTTG1 interactome in seminoma cell lines showing different PTTG1 nuclear levels correlated with invasive properties. A proteomic approach upon PTTG1 immunoprecipitation uncovered new specific securin interactors. Western blot, confocal microscopy, cytoplasmic/nuclear fractionation, sphere-forming assay, and Atlas database interrogation were performed to validate the proteomic results and to investigate the interplay between PTTG1 and newly uncovered partners. We observed that spectrin beta-chain (SPTBN1) and PTTG1 were cofactors, with SPTBN1 anchoring the securin in the cytoplasm. SPTBN1 downregulation determined PTTG1 nuclear translocation, promoting its invasive capability. Moreover, a PTTG1 deletion mutant lacking SPTBN1 binding was strongly localized in the nucleus. The Atlas database revealed that seminomas that contained higher nuclear PTTG1 levels showed significantly lower SPTBN1 levels in comparison to non-seminomas. In human seminoma specimens, we found a strong PTTG1/SPTBN1 colocalization that decreases in areas with nuclear PTTG1 distribution. Overall, these results suggest that SPTBN1, along with PTTG1, is a potential prognostic factor useful in the clinical management of seminoma.

Published
2023

34. Label-free metabolic clustering through unsupervised pixel classification of multiparametric fluorescent images

Author

Bianchetti, G., Ciccarone, F., Ciriolo, M. R., De Spirito, M., Pani, G., Maulucci, G., Bianchetti G., De Spirito M. (ORCID:0000-0003-4260-5107), Pani G. (ORCID:0000-0001-7133-8728), Maulucci G. (ORCID:0000-0002-2154-319X), Bianchetti, G., Ciccarone, F., Ciriolo, M. R., De Spirito, M., Pani, G., Maulucci, G., Bianchetti G., De Spirito M. (ORCID:0000-0003-4260-5107), Pani G. (ORCID:0000-0001-7133-8728), and Maulucci G. (ORCID:0000-0002-2154-319X)

Abstract

Autofluorescence microscopy is a promising label-free approach to characterize NADH and FAD metabolites in live cells, with potential applications in clinical practice. Although spectrally resolved lifetime imaging techniques can acquire multiparametric information about the biophysical and biochemical state of the metabolites, these data are evaluated at the whole-cell level, thus providing only limited insights in the activation of metabolic networks at the microscale. To overcome this issue, here we introduce an artificial intelligence-based analysis that, leveraging the multiparametric content of spectrally resolved lifetime images, allows to detect and classify, through an unsupervised learning approach, metabolic clusters, which are regions having almost uniform metabolic properties. This method contextually detects the cellular mitochondrial turnover and the metabolic activation state of intracellular compartments at the pixel level, described by two functions: the cytosolic activation state (CAF) and the mitochondrial activation state (MAF). This method was applied to investigate metabolic changes elicited in the breast cancer cell line MCF-7 by specific inhibitors of glycolysis and electron transport chain, and by the deregulation of a specific mitochondrial enzyme (ACO2) leading to defective aerobic metabolism associated with tumor growth. In this model, mitochondrial fraction undergoes to a 13% increase upon ACO2 overexpression and the MAF function changes abruptly by altering the metabolic state of about the 25% of the mitochondrial pixels.

Published
2021

45. Hippocampal Sparing Radiotherapy in adults with Primary Brain Tumors: A comparative planning and dosimetric study using IMPT, IMRT and 3DCRT

Author

Aka, P, Taylor, R, Hugtenburg, R, Lambert, J, Powell, J, Bevolo, T, Gao, M, Gondi, V, Hartsell, W.H, Bolsi, A, Beer, J, Belosi, M.F, Siewert, D, Lomax, A.J, Weber, D.C, Huang, Y.J, Huang, C.C, Chao, P.J, Liu, C, Shang, H, Ding, X, Wang, Y, Mammar, H, Froelich, Sébastien, Alapetite, Claire, Bolle, Stéphanie, Calugaru, Valentin, Feuvret, Loic, Helfre, Sylvie, Champion, Laurence, Goudjil, Farid, Dendal, Remi, Engelholm, S.A, Munck Af Rosenschold, P, Kristensen, I, Smulders, B, Muhic, A, Alkner, S, Jacob, E, Engelholm, S, Aljabab, S, Lui, A, Wong, T, Liao, J, Laramore, G, Parvathaneni, U, Kharouta, M, Pidikiti, R, Jesseph, F, Smith, M, Dobbins, D, Mattson, D, Choi, S, Mansur, D, Machtay, M, Bhatt, A, Lütgendorf-Caucig, C, Dunavölgyi, R, Georg, P, Perpar, A, Fussl, C, Konstantinovic, R, Ulrike, M, Piero, F, Eugen, H, Vidal, M, Gerard, A, Barnel, C, Maneval, D, Herault, J, Claren, A, Doyen, J, Dendale, R, Toutee, A, Pasquie, I, Goudjil, F, Lumbroso Lerouic, L, Levy, C, Desjardins, L, Cassoux, N, Elisei, G, Pella, A, Calvi, G, Ricotti, R, Tagaste, B, Valvo, F, Ciocca, M, Via, R, Mastella, E, Baroni, G, Saotome, N, Yonai, S, Makishima, H, Hara, Y, Inaniwa, T, Sakama, M, Kanematsu, N, Tsuji, H, Furukawa, T, Shirai, T, Sauerwein, W, Finger, P.T, Gallie, B, Gavrylyuk, Y, Thariat, J, Salleron, J, Maschi, C, Fevrier, E, Caujolle, J.P, Hofverberg, P, Angellier, G, Peyrichon, M.L, Breneman, J, Esslinger, H, Pater, L, Vatner, R, Habrand, J.L, Stefan, D, Lesueur, P, Kao, W, Véla, A, Geffrelot, J, Tessonnier, T, Balosso, J, Mahé, M.A, Lim, P.S, Rompokos, V, Chang, Y.C, Royle, G, Gaze, M, Gains, J, Vennarini, S, Francesco, F, Rombi, B, Amichetti, M, Schwarz, M, Lorentini, S, Mee, T, Burnet, N.G, Crellin, A, Kirkby, N.F, Smith, E, Kirkby, K.J, Roggio, M, Buwenge, M, Melchionda, F, Ammendolia, I, Ronchi, L, Cammelli, S, Morganti, A.G, Youn, S.H, Kim, J.Y, Park, H.J, Shin, S.H, Lee, S.H, Hong, E.K, Czerska, K, Winczura, P, Wejs-Maternik, J, Blukis, A, Antonowicz-Szydlowska, M, Rucinski, A, Olko, P, Badzio, A, Kopec, R, Franceschini, D, Cozzi, L, De Rose, F, Meattini, I, Fogliata, A, Cozzi, S, Becherini, C, Tomatis, S, Livi, L, Scorsetti, M, Garda, A, Fattahi, S, Michel, A, Mutter, R, Yan, E, Park, S, Corbin, K, Giap, H, LAM, W.W, Geng, H, Tang, K.K, Lee, T.Y, Kong, C.W, Yang, B, Chiu, T.L, Cheung, K.Y, Yu, S.K, Ma, M, Gao, X, Zhao, Z, Zhao, B, Mullikin, T, Routman, D, Yu, J, Greco, K, Fagundes, M, Shan, J, Daniels, T, Rule, W, DeWees, T, Hu, Y, Bues, M, Sio, T, Liu, W, chenbin, L, yuehu, P, yuenan, W, Bai, Y, Gao, X.S, Zhao, Z.L, Ma, M.W, Ren, X.Y, Salem, A, Woolf, D, Aznar, M, Azadeh, A, Eccles, C, Charlwood, F, Faivre-Finn, C, Teoh, S, Fiorini, F, George, B, Vallis, K, Van den Heuvel, F, Huang, E.Y, Juang, P.J, Pan, S, Hawkins, M, Clarke, M, Lowe, M, Radhakrishna, G, Schaub, S, Bowen, S, Nyflot, M, Chapman, T, Apisarnthanarax, S, Vitek, P, Kubes, J, Vondracek, V, Vinakurau, S, Zamecnik, L, Vitolo, V, Barcellini, A, Brugnatelli, S, Cobianchi, L, Vanoli, A, Fossati, P, Facoetti, A, Dionigi, P, Orecchia, R, Iannalfi, A, Vischioni, B, Ronchi, S, D’Ippolito, E, Petrucci, R, Yamaguchi, H, Honda, M, Hamada, K, Todate, Y, Seto, I, Suzuki, M, Wada, H, Murakami, M, Yu, Z, Zheng, W, Lien-Chun, L, Zhengshan, H, Qing, Z, Jiade, L, Guoliang, J, Fiore, M.R, D'Ippolito, E, Fukumitsu, N, Hayakawa, T, Yamashita, T, Mima, M, Demizu, Y, Suzuki, T, Soejima, T, Hartsell, W, Collins, S, Casablanca, V, Mihalcik, S, Brennan, E, Van Nispen, A, Corbett, A, Mohammed, N, Lee, P, van Nispen, A, Liang, Y.S, Mein, S, Kopp, B, Choi, K, Haberer, T, Debus, J, Abdollahi, A, Mairani, A, Ogino, H, Iwata, H, Hashimoto, S, Nakajima, K, Hattori, Y, Nomura, K, Shibamoto, Y, Li, P, Wu, S, Deng, L, Zhang, G, Zhang, Q, Fu, S, Yang, Z, Zhang, Y, Sasaki, R, Okimoto, T, Akasaka, H, Miyawaki, D, Yoshida, K, Wang, T, Komatsu, S, Fukumoto, T, Shuang, W, Xin, C, zhengshan, H, Shen, F, Vorobyov, N, Andreev, G, Martynova, N, Lyubinsky, A, Kubasov, A, Chen, J, Ma, N, Lu, Y, Zhao, J, Shahnazi, K, Lu, J, Jiang, G, Mao, J, Walser, M, Bojaxhiu, B, Kawashiro, S, Tran, S, Pica, A, Bachtiary, B, Weber, D, Gaito, S, Abravan, A, Richardson, J, Colaco, R, Saunders, D, Brennan, B, Petersen, I, Ahmed, S, Laack, N, Mizoe, J.E, Iizumi, T, Minohara, S, Kusano, Y, Matsuzaki, Y, Tsuchida, K, Serizawa, I, Yoshida, D, Katoh, H, Sakurai, H, Tujii, H, Kim, T.H, Park, J.W, Bo Hyun, K, Hyunjung, K, Sung Ho, M, Sang Soo, K, Sang Myung, W, Young-Hwan, K, Woo Jin, L, Dae Yong, K, Hong, Z, Wang, Z, Koroulakis, A, Molitoris, J, Kaiser, A, Hanna, N, Jiang, Y, Regine, W, DeCesaris, C.M, Choi, J.I, Carr, S.R, Burrows, W.M, Regine, W.F, Simone, C.B, Aihara, T, Hiratsuka, J, Kamitani, N, Higashino, M, Kawata, R, Kumada, H, Ono, K, Chou, Y.C, Dippolito, E, Bonora, M, Alterio, D, Gandini, S, Jereczeck, B.A, Kelly, C, Dobeson, C, Iqbal, S, Chatterjee, S, Hague, C, Li, T, Lin, A, Lukens, J, Slevin, N, Thomson, D, van Herk, M, West, C, Teo, K, Jeans, E, Manzar, G, Patel, S, Ma, D, Lester, S, Foote, R, Friborg, J, Jensen, K, Hansen, C.R, Andersen, E, Andersen, M, Eriksen, J.G, Johansen, J, Overgaard, J, Grau, C, Dědečková, K, Vítek, P, Ondrová, B, Sláviková, S, Zapletalová, S, Zapletal, R, Vondráček, V, Rotnáglová, E, Kwanghyun, J, Woojin, L, Dongryul, O, Yong Chan, A, Paudel, N, Schmidt, S, Ruckman, M, Gans, S, Stauffer, M, Helenowski, I, Patel, U, Samant, S, Gentile, M, Damico, N, Yao, M, Shuja, M, Routman, D.M, Foote, R.L, Garces, Y.I, Neben-Wittich, M.A, Patel, S.H, McGee, L.A, Harmsen, W.S, Ma, D.J, Sommat, K, Tong, A.K.T, Hu, J, Ong, A.L.K, Wang, F, Sin, S.Y, Wee, T.S, Tan, W.K, Fong, K.W, Soong, Y.L, Wallace, N, Fredericks, S, Fitzgerald, T, Vernimmen, F, Petringa, G, Cirrone, P, Agosteo, S, Attili, A, Cammarata, F.P, Cuttone, G, Conte, V, La Tessa, C, Manti, L, Rosenfeld, A, Lojacono, P.A, Hennings, F, Fattori, G, Peroni, M, Lomax, A, Hrbacek, J, Nguyen, H.G, Bach Cuadra, M, Sznitman, R, Schalenbourg, A, Pflaeger, A, Weber, A, Seidel, S, Stark, R, Heufelder, J, Mailhot Vega, R, Bradley, J, Lockney, N, Macdonald, S, Liang, X, Mazal, A, Mendenhall, N, Sher, D, Korreman, S.S, Andreasen, S, Petersen, J.B, Offersen, B.V, Gergelis, K, Jethwa, K, Whitaker, T, Shiraishi, S, Shumway, D, Press, R, Shelton, J, Zhang, C, Dang, Q, Tian, S, Shu, T, Seldon, C, Jani, A, Zhou, J, McDonald, M, Gort, E, Beukema, J.C, Spijkerman-Bergsma, M.J, Both, S, Langendijk, J.A, Matysiak, W.P, Brouwer, C.L, Baba, K, Numajiri, H, Murofushi, K, Oshiro, Y, Mizumoto, M, Onishi, K, Nonaka, T, Ishikawa, H, Okumura, T, Dominietto, M, Adam, K, Ahlhelm, F.J, Safai, S, Abdul-Jabbar, L, Song, J, Tseng, Y. 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Subjects
Biology: Biological Differences between Carbon, Proton and Photons Poster Discussion SessionsPTC58-0642, Physics: Absolute and Relative DosimetryPTC58-0180, Biology: Biology and Clinical InterfacePTC58-0685, Physics: Commissioning New FacilitiesPTC58-0385, Physics: 4D Treatment and DeliveryPTC58-0546, Clinics: EyePTC58-0714, Biology: Biological Differences between Carbon, Proton and Photons Poster Discussion SessionsPTC58-0528, Physics: Quality Assurance and VerificationPTC58-0507, Oral AbstractsPhysics: Dose Calculation and OptimisationPTC58-0661, Biology: Translational and Biomarkers Poster Discussion SessionsPTC58-0221, Oral AbstractsPhysics: Dose Calculation and OptimisationPTC58-0531, Oral AbstractsPhysics: Dose Calculation and OptimisationPTC58-0653, Biology: Drug and Immunotherapy CombinationsPTC58-0163, Clinics: Sarcoma - LymphomaPTC58-0055, Biology: Drug and Immunotherapy CombinationsPTC58-0166, Clinics: CNS / Skull BasePTC58-0198, Physics: Treatment PlanningPTC58-0421, Clinics: PediatricsPTC58-0560, General: New HorizonsPTC58-0709, Physics: Treatment PlanningPTC58-0664, Clinics: Eye / Breast / Pelvis Poster Discussion SessionsPTC58-0286, Physics: Treatment PlanningPTC58-0666, Biology: Translational and Biomarkers Poster Discussion SessionsPTC58-0346, Physics: Treatment PlanningPTC58-0547, Physics: Treatment PlanningPTC58-0308, Physics: Treatment PlanningPTC58-0549, Physics: Beam Delivery and Nozzle Design Poster Discussion SessionsPTC58-0111, Physics: Absolute and Relative DosimetryPTC58-0050, Biology: Enhanced Biology in Treatment Planning Poster Discussion SessionsPTC58-0587, Biology: Biology and Clinical InterfacePTC58-0454, Physics: Absolute and Relative DosimetryPTC58-0052, Physics: Commissioning New FacilitiesPTC58-0395, Physics: 4D Treatment and DeliveryPTC58-0534, Physics: Dose Calculation and OptimisationPTC58-0072, Physics: 4D Treatment and DeliveryPTC58-0533, Physics: 4D Treatment and DeliveryPTC58-0538, Physics: Commissioning New Facilities Poster Discussion SessionsPTC58-0113, Physics: Quality Assurance and VerificationPTC58-0633, Physics: Treatment PlanningPTC58-0431, Physics: Beam Delivery and Nozzle DesignPTC58-0230, Biology: Mathematical Modelling SimulationPTC58-0179, Clinics: Head and Neck / EyePTC58-0365, Physics: Treatment PlanningPTC58-0319, Biology: Translational and Biomarkers Poster Discussion SessionsPTC58-0697, Biology: Biology and Clinical InterfacePTC58-0663, Physics: Commissioning New FacilitiesPTC58-0240, Physics: Adaptive TherapyPTC58-0177, Physics: Commissioning New FacilitiesPTC58-0363, Physics: Commissioning New FacilitiesPTC58-0487, Physics: 4D Treatment and DeliveryPTC58-0209, Physics: 4D Treatment and DeliveryPTC58-0206, Clinics: CNS / Skull BasePTC58-0294, Physics: Commissioning New FacilitiesPTC58-0127, Biology: Mathematical Modelling SimulationPTC58-0068, Physics: Treatment Planning Poster Discussion SessionsPTC58-0062, Physics: 4D Treatment and DeliveryPTC58-0692, Physics: Quality Assurance and VerificationPTC58-0723, Physics: Commissioning New Facilities Poster Discussion SessionsPTC58-0494, Physics: Treatment PlanningPTC58-0643, Physics: Treatment PlanningPTC58-0521, Physics: Treatment PlanningPTC58-0402, Physics: Treatment PlanningPTC58-0405, Clinics: Head and Neck / EyePTC58-0273, Clinics: GIPTC58-0397, Physics: Treatment PlanningPTC58-0648, Biology: Enhanced Biology in Treatment Planning Poster Discussion SessionsPTC58-0489, Physics: Quality Assurance and VerificationPTC58-0617, Physics: Quality Assurance and VerificationPTC58-0616, Physics: Dose Calculation and Optimisation Poster Discussion SessionsPTC58-0668, Clinics: CNS / Skull BasePTC58-0188, Oral AbstractsPhysics: Dose Calculation and OptimisationPTC58-0625, Physics: Treatment PlanningPTC58-0654, Physics: Treatment PlanningPTC58-0655, Biology: Drug and Immunotherapy Combinations Poster Discussion SessionsPTC58-0133, Clinics: PediatricsPTC58-0313, Physics: Treatment PlanningPTC58-0659, Poster AbstractsClinics: CNSPTC58-0290, Physics: Commissioning New FacilitiesPTC58-0064, Physics: Adaptive TherapyPTC58-0396, Physics: Dose Calculation and OptimisationPTC58-0281, Physics: Quality Assurance and VerificationPTC58-0427, Physics: Quality Assurance and VerificationPTC58-0669, General: New Horizons SessionPTC58-0191, Physics: Dose Calculation and Optimisation Poster Discussion SessionsPTC58-0217, Physics: Quality Assurance and VerificationPTC58-0303, Physics: Quality Assurance and VerificationPTC58-0665, Clinics: Sarcoma - LymphomaPTC58-0495, Physics: Dose Calculation and OptimisationPTC58-0398, Physics: Quality Assurance and VerificationPTC58-0667, Physics: Quality Assurance and VerificationPTC58-0425, Physics: Quality Assurance and VerificationPTC58-0541, Physics: Treatment PlanningPTC58-0584, Physics: Quality Assurance and VerificationPTC58-0540, Biology: Drug and Immunotherapy Combinations Poster Discussion SessionsPTC58-0163, Physics: Treatment PlanningPTC58-0224, Physics: Treatment PlanningPTC58-0229, Clinics: PediatricsPTC58-0249, Physics: Beam Delivery and Nozzle Design Poster Discussion SessionsPTC58-0555, Clinics: PediatricPTC58-0463, Physics: Commissioning New Facilities Poster Discussion SessionsPTC58-0556, Physics: Absolute and Relative DosimetryPTC58-0498, Physics: Commissioning New FacilitiesPTC58-0078, Physics: Dose Calculation and OptimisationPTC58-0270, Physics: Dose Calculation and OptimisationPTC58-0032, Physics: Dose Calculation and OptimisationPTC58-0274, Physics: 4D Treatment and DeliveryPTC58-0614, Physics: Dose Calculation and OptimisationPTC58-0026, Clinics: Head and Neck / EyePTC58-0280, Clinics: Eye / Breast / Pelvis Poster Discussion SessionsPTC58-0091, Physics: Treatment PlanningPTC58-0593, Biology: Drug and Immunotherapy CombinationsPTC58-0012, Physics: Dose Calculation and OptimisationPTC58-0025, Physics: Dose Calculation and OptimisationPTC58-0146, Clinics: Sarcoma - LymphomaPTC58-0261, Physics: Treatment PlanningPTC58-0110, Clinics: Lung / Sarcoma / LymphomaPTC58-0733, Physics: Quality Assurance and VerificationPTC58-0554, Physics: Treatment PlanningPTC58-0597, Physics: Dose Calculation and Optimisation Poster Discussion SessionsPTC58-0330, Physics: Treatment PlanningPTC58-0115, Physics: Treatment PlanningPTC58-0598, Physics: Absolute and Relative DosimetryPTC58-0040, Physics: Absolute and Relative DosimetryPTC58-0282, Biology: Enhanced Biology in Treatment Planning Poster Discussion SessionsPTC58-0399, Physics: Absolute and Relative DosimetryPTC58-0283, Physics: Commissioning New Facilities Poster Discussion SessionsPTC58-0569, Clinics: GUPTC58-0647, Biology: Biological Differences between Carbon, Proton and Photons Poster Discussion SessionsPTC58-0506, Physics: Commissioning New FacilitiesPTC58-0047, Physics: Dose Calculation and OptimisationPTC58-0067, Clinics: GUPTC58-0409, Physics: Dose Calculation and OptimisationPTC58-0065, Biology: BNCT Poster Discussion SessionsPTC58-0586, Physics: Absolute and Relative Dosimetry PTC58-0393, Physics: Image GuidancePTC58-0712, Physics: Quality Assurance and VerificationPTC58-0645, Physics: Treatment PlanningPTC58-0683, Biology: BNCT Poster Discussion SessionsPTC58-0107, Physics: Treatment Planning Poster Discussion SessionsPTC58-0266, Physics: Monitoring and Modelling MotionPTC58-0530, Biology: BNCT Poster Discussion SessionsPTC58-0341, Physics: Commissioning New FacilitiesPTC58-0172, Physics: Commissioning New Facilities Poster Discussion SessionsPTC58-0456, Physics: Dose Calculation and OptimisationPTC58-0170, Physics: Commissioning New Facilities Poster Discussion SessionsPTC58-0458, Physics: Absolute and Relative DosimetryPTC58-0034, Physics: Quality Assurance and VerificationPTC58-0417, Physics: Quality Assurance and VerificationPTC58-0413, Physics: Treatment Planning Poster Discussion SessionsPTC58-0492, Physics: Dose Calculation and OptimisationPTC58-0168, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0724, Physics: Treatment PlanningPTC58-0694, Physics: Adaptive TherapyPTC58-0005, Physics: Treatment PlanningPTC58-0696, Physics: Treatment PlanningPTC58-0453, Physics: Adaptive TherapyPTC58-0366, Clinics: BreastPTC58-0197, Physics: Beam Delivery and Nozzle DesignPTC58-0652, Physics: Treatment Planning Poster Discussion SessionsPTC58-0017, Physics: Treatment PlanningPTC58-0338, Clinics: Head and Neck / EyePTC58-0539, General: New Horizons SessionPTC58-0390, Physics: Image Guidance Poster Discussion SessionsPTC58-0651, General: New HorizonsPTC58-0660, Physics: Dose Calculation and OptimisationPTC58-0360, Physics: Image GuidancePTC58-0297, Physics: 4D Treatment and DeliveryPTC58-0147, Scientific: RTTPTC58-0388, Physics: Dose Calculation and OptimisationPTC58-0484, General: New HorizonsPTC58-0301, Physics: Dose Calculation and OptimisationPTC58-0485, General: New HorizonsPTC58-0304, Physics: 4D Treatment and Delivery Poster Discussion SessionsPTC58-0532, Clinics: GIPTC58-0575, General: New HorizonsPTC58-0306, Physics: Quality Assurance and VerificationPTC58-0589, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0344, Physics: Quality Assurance and VerificationPTC58-0225, Physics: Treatment PlanningPTC58-0381, Physics: Quality Assurance and VerificationPTC58-0467, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0585, Physics: Commissioning New FacilitiesPTC58-0416, Physics: Quality Assurance and VerificationPTC58-0228, Physics: Quality Assurance and VerificationPTC58-0348, Physics: Dose Calculation and OptimisationPTC58-0234, Physics: Quality Assurance and VerificationPTC58-0101, Physics: Treatment PlanningPTC58-0386, Physics: Dose Calculation and OptimisationPTC58-0118, Physics: Treatment PlanningPTC58-0265, Physics: Dose Calculation and OptimisationPTC58-0119, Clinics: GIPTC58-0218, Physics: Treatment PlanningPTC58-0267, Physics: Treatment PlanningPTC58-0387, Clinics: BreastPTC58-0142, Physics: Treatment PlanningPTC58-0269, Physics: Beam Delivery and Nozzle DesignPTC58-0620, Clinics: PediatricsPTC58-0048, Physics: Quality Assurance and VerificationPTC58-0220, Physics: Quality Assurance and VerificationPTC58-0461, Physics: Treatment PlanningPTC58-0029, Physics: Absolute and Relative DosimetryPTC58-0571, Physics: Image GuidancePTC58-0046, Clinics: GUPTC58-0557, Physics: Absolute and Relative DosimetryPTC58-0211, Oral AbstractsPhysics: Dose Calculation and OptimisationPTC58-0131, Oral AbstractsPhysics: Dose Calculation and OptimisationPTC58-0373, General: New HorizonsPTC58-0411, Physics: Dose Calculation and OptimisationPTC58-0595, Clinics: CNS / Skull BasePTC58-0361, General: New HorizonsPTC58-0414, General: New HorizonsPTC58-0537, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0628, Physics: Treatment PlanningPTC58-0271, Physics: Commissioning New FacilitiesPTC58-0307, Physics: Quality Assurance and VerificationPTC58-0359, Physics: Quality Assurance and VerificationPTC58-0354, General: New HorizonsPTC58-0419, Physics: Treatment PlanningPTC58-0035, Biology: BNCTPTC58-0474, Clinics: GIPTC58-0460, Biology: BNCTPTC58-0596, Clinics: GIPTC58-0222, Physics: Image GuidancePTC58-0193, Clinics: PediatricPTC58-0312, Clinics: GUPTC58-0441, Clinics: LungPTC58-0701, Clinics: EyePTC58-0536, Clinics: GUPTC58-0205, Physics: Dose Calculation and OptimisationPTC58-0140, Clinics: GUPTC58-0208, Physics: Dose Calculation and OptimisationPTC58-0020, Physics: Image GuidancePTC58-0195, Poster AbstractsClinics: CNSPTC58-0717, Physics: Quality Assurance and VerificationPTC58-0325, Physics: Dose Calculation and OptimisationPTC58-0015, Physics: Commissioning New FacilitiesPTC58-0634, General: New HorizonsPTC58-0646, Physics: Quality Assurance and VerificationPTC58-0566, Physics: Dose Calculation and OptimisationPTC58-0134, Physics: Dose Calculation and OptimisationPTC58-0376, Biology: Mathematical Modelling SimulationPTC58-0462, Biology: BNCTPTC58-0567, General: New HorizonsPTC58-0527, Physics: Treatment PlanningPTC58-0482, Clinics: GI, GU, BreastPTC58-0693, Physics: Commissioning New FacilitiesPTC58-0518, Physics: Quality Assurance and VerificationPTC58-0686, Physics: Quality Assurance and VerificationPTC58-0202, Physics: Quality Assurance and VerificationPTC58-0322, Physics: Quality Assurance and VerificationPTC58-0564, Physics: Quality Assurance and VerificationPTC58-0680, Physics: Treatment PlanningPTC58-0247, Physics: Quality Assurance and VerificationPTC58-0682, Physics: Quality Assurance and VerificationPTC58-0440, Biology: Translational and BiomarkersPTC58-0514, Physics: Beam Delivery and Nozzle Design Poster Discussion SessionsPTC58-0178, Clinics: EyePTC58-0520, Physics: Absolute and Relative DosimetryPTC58-0231, Clinics: Head and Neck / EyePTC58-0424, Physics: Absolute and Relative DosimetryPTC58-0471, Physics: Absolute and Relative DosimetryPTC58-0356, Physics: Dose Calculation and OptimisationPTC58-0491, Physics: Dose Calculation and OptimisationPTC58-0250, Physics: Commissioning New FacilitiesPTC58-0650, Biology: Biology and Clinical InterfacePTC58-0719, Physics: Absolute and Relative DosimetryPTC58-0232, Physics: Absolute and Relative DosimetryPTC58-0353, General: New HorizonsPTC58-0511, Physics: Quality Assurance and VerificationPTC58-0219, Physics: Absolute and Relative DosimetryPTC58-0238, General: New HorizonsPTC58-0512, Physics: 4D Treatment and Delivery Poster Discussion SessionsPTC58-0401, Clinics: PediatricPTC58-0688, Physics: Quality Assurance and VerificationPTC58-0457, Physics: Quality Assurance and VerificationPTC58-0214, Physics: Quality Assurance and VerificationPTC58-0459, General: New HorizonsPTC58-0516, Physics: Treatment PlanningPTC58-0372, Physics: Treatment PlanningPTC58-0011, Physics: Treatment PlanningPTC58-0254, Physics: Quality Assurance and VerificationPTC58-0332, Clinics: CNS / Skull BasePTC58-0468, Biology: Mathematical Modelling SimulationPTC58-0357, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0649, Physics: Dose Calculation and OptimisationPTC58-0006, Physics: Quality Assurance and VerificationPTC58-0212, Physics: Image Guidance Poster Discussion SessionsPTC58-0565, Physics: Treatment PlanningPTC58-0018, Physics: Treatment PlanningPTC58-0019, Clinics: BreastPTC58-0576, Clinics: Head and Neck / EyePTC58-0335, Clinics: Head and Neck / EyePTC58-0577, General: New HorizonsPTC58-0621, Physics: Absolute and Relative DosimetryPTC58-0426, Physics: Commissioning New Facilities Poster Discussion SessionsPTC58-0268, Physics: Absolute and Relative DosimetryPTC58-0423, Physics: Treatment PlanningPTC58-0184, Physics: Quality Assurance and VerificationPTC58-0149, Clinics: GIPTC58-0378, Clinics: GIPTC58-0257, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0662, General: New HorizonsPTC58-0627, Physics: Treatment PlanningPTC58-0186, Physics: Treatment PlanningPTC58-0185, Physics: Quality Assurance and VerificationPTC58-0144, Biology: BNCT Poster Discussion SessionsPTC58-0602, Physics: Treatment PlanningPTC58-0189, Physics: Dose Calculation and OptimisationPTC58-0315, Clinics: Head and neckPTC58-0300, General: New Horizons SessionPTC58-0347, Physics: Image GuidancePTC58-0082, Clinics: BreastPTC58-0443, Physics: 4D Treatment and Delivery Poster Discussion SessionsPTC58-0629, Physics: Adaptive Therapy Poster Discussion SessionsPTC58-0007, Physics: Commissioning New FacilitiesPTC58-0472, Clinics: GI, GU, BreastPTC58-0515, Physics: Dose Calculation and Optimisation Poster Discussion SessionsPTC58-0606, Oral AbstractsPhysics: Dose Calculation and OptimisationPTC58-0450, Physics: Absolute and Relative DosimetryPTC58-0657, Physics: Dose Calculation and OptimisationPTC58-0551, Physics: Treatment PlanningPTC58-0192, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0675, Physics: Treatment PlanningPTC58-0194, Physics: Dose Calculation and OptimisationPTC58-0544, Physics: Treatment PlanningPTC58-0199, Physics: Quality Assurance and VerificationPTC58-0037, Oral AbstractsPhysics: Dose Calculation and OptimisationPTC58-0207, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0434, Physics: Quality Assurance and VerificationPTC58-0036, Physics: Quality Assurance and VerificationPTC58-0278, Physics: Quality Assurance and VerificationPTC58-0394, Physics: Quality Assurance and VerificationPTC58-0151, Physics: Quality Assurance and VerificationPTC58-0154, Physics: Dose Calculation and OptimisationPTC58-0428, Clinics: BreastPTC58-0116, Biology: Enhanced Biology in Treatment Planning Poster Discussion SessionsPTC58-0435, Physics: Commissioning New FacilitiesPTC58-0681, Physics: Absolute and Relative DosimetryPTC58-0323, Physics: Dose Calculation and OptimisationPTC58-0583, Physics: Absolute and Relative DosimetryPTC58-0448, Clinics: CNS / Skull BasePTC58-0251, General: New HorizonsPTC58-0721, Physics: Absolute and Relative DosimetryPTC58-0203, Physics: Dose Calculation and OptimisationPTC58-0455, Physics: 4D Treatment and DeliveryPTC58-0130, Physics: Commissioning New FacilitiesPTC58-0679, Physics: Absolute and Relative DosimetryPTC58-0329, General: New HorizonsPTC58-0604, Physics: Absolute and Relative DosimetryPTC58-0449, Clinics: CNS / Skull BasePTC58-0132, General: New HorizonsPTC58-0607, Physics: Quality Assurance and VerificationPTC58-0122, Physics: Quality Assurance and VerificationPTC58-0243, Physics: Treatment PlanningPTC58-0165, Oral AbstractsPhysics: Dose Calculation and OptimisationPTC58-0437, Physics: 4D Treatment and DeliveryPTC58-0377, Physics: Quality Assurance and VerificationPTC58-0125, Physics: Quality Assurance and VerificationPTC58-0245, Physics: Dose Calculation and OptimisationPTC58-0337, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0334, Physics: Quality Assurance and VerificationPTC58-0121, General: New Horizons SessionPTC58-0563, General: New Horizons SessionPTC58-0321, Clinics: Head and Neck / EyePTC58-0477, Physics: Quality Assurance and VerificationPTC58-0480, Clinics: GUPTC58-0010, Clinics: EyePTC58-0684, Clinics: GUPTC58-0496, Clinics: Head and neckPTC58-0676, Clinics: GUPTC58-0137, Physics: Beam Delivery and Nozzle Design Poster Discussion SessionsPTC58-0256, Physics: 4D Treatment and DeliveryPTC58-0117, Physics: Absolute and Relative DosimetryPTC58-0552, Physics: Absolute and Relative DosimetryPTC58-0310, Physics: Absolute and Relative DosimetryPTC58-0672, Physics: Absolute and Relative DosimetryPTC58-0436, Physics: Dose Calculation and OptimisationPTC58-0452, Physics: Dose Calculation and OptimisationPTC58-0331, Physics: Commissioning New FacilitiesPTC58-0213, Biology: Mathematical Modelling SimulationPTC58-0272, Clinics: EyePTC58-0326, Physics: Commissioning New FacilitiesPTC58-0568, Physics: Dose Calculation and OptimisationPTC58-0444, Physics: Quality Assurance and VerificationPTC58-0379, Physics: Treatment Planning Poster Discussion SessionsPTC58-0095, Physics: Treatment PlanningPTC58-0053, Physics: Absolute and Relative DosimetryPTC58-0438, Physics: Absolute and Relative DosimetryPTC58-0317, Physics: Quality Assurance and VerificationPTC58-0497, Physics: Quality Assurance and VerificationPTC58-0375, Physics: Treatment PlanningPTC58-0056, Physics: 4D Treatment and DeliveryPTC58-0124, Clinics: GIPTC58-0009, Physics: Quality Assurance and VerificationPTC58-0014, Physics: Quality Assurance and VerificationPTC58-0374, Clinics: LungPTC58-0727, General: New Horizons SessionPTC58-0578, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0470, Clinics: LungPTC58-0204, Clinics: Head and neckPTC58-0227, Clinics: LungPTC58-0446, Physics: Quality Assurance and VerificationPTC58-0190, Clinics: Eye / Breast / Pelvis Poster Discussion SessionsPTC58-0609, Clinics: LungPTC58-0689, General: New HorizonsPTC58-0021, General: New HorizonsPTC58-0262, Biology: BNCT Poster Discussion SessionsPTC58-0081, Clinics: GIPTC58-0726, General: New HorizonsPTC58-0145, Physics: Image GuidancePTC58-0573, General: New HorizonsPTC58-0027, General: New HorizonsPTC58-0028, Biology: Mathematical Modelling and SimulationPTC58-0148, Physics: Dose Calculation and OptimisationPTC58-0635, Physics: Image GuidancePTC58-0215, Physics: Image GuidancePTC58-0336, Poster AbstractsClinics: CNSPTC58-0535, Physics: Quality Assurance and VerificationPTC58-0187, Biology: BNCT Poster Discussion SessionsPTC58-0084, General: New Investigator SessionPTC58-0339, General: New Horizons SessionPTC58-0420, Physics: Treatment Planning Poster Discussion SessionsPTC58-0523, Biology: BNCT Poster Discussion SessionsPTC58-0088, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0112, Physics: Quality Assurance and VerificationPTC58-0182, Clinics: Eye / Breast / Pelvis Poster Discussion SessionsPTC58-0615, Physics: Quality Assurance and VerificationPTC58-0080, Biology: BNCTPTC58-0085, Physics: Adaptive Therapy Poster Discussion SessionsPTC58-0722, General: New HorizonsPTC58-0253, General: New HorizonsPTC58-0255, Clinics: PediatricPTC58-0703, General: New HorizonsPTC58-0499, Physics: Image Guidance Poster Discussion SessionsPTC58-0380, General: New HorizonsPTC58-0259, Clinics: GI, GU, BreastPTC58-0288, Clinics: GI, GU, BreastPTC58-0045, Physics: Absolute and Relative DosimetryPTC58-0619, Clinics: PediatricPTC58-0707, Physics: Quality Assurance and VerificationPTC58-0196, Physics: Quality Assurance and VerificationPTC58-0074, Physics: Quality Assurance and VerificationPTC58-0077, Biology: BNCT Poster Discussion SessionsPTC58-0073, Biology: BNCTPTC58-0075, Biology: Biological Differences between Carbon, Proton and Photons Poster Discussion SessionsPTC58-0093, Clinics: GUPTC58-0161, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0371, Physics: Monitoring and Modelling MotionPTC58-0181, General: New HorizonsPTC58-0120, General: New HorizonsPTC58-0362, General: New HorizonsPTC58-0364, Physics: Image GuidancePTC58-0473, Scientific: RTTPTC58-0641, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0296, General: New HorizonsPTC58-0004, General: New HorizonsPTC58-0128, Clinics: BreastPTC58-0316, Physics: 4D Treatment and Delivery Poster Discussion SessionsPTC58-0236, General: New HorizonsPTC58-0008, General: New Investigator SessionPTC58-0673, Physics: Quality Assurance and VerificationPTC58-0167, Physics: Quality Assurance and VerificationPTC58-0289, Physics: Quality Assurance and VerificationPTC58-0284, General: New Horizons SessionPTC58-0522, Physics: Quality Assurance and VerificationPTC58-0164, Physics: Quality Assurance and VerificationPTC58-0285, Clinics: Eye / Breast / Pelvis Poster Discussion SessionsPTC58-0623, Clinics: Eye / Breast / Pelvis Poster Discussion SessionsPTC58-0502, Clinics: GUPTC58-0293, Biology: Translational and BiomarkersPTC58-0599, Biology: BNCTPTC58-0063, Clinics: LungPTC58-0656, General: New HorizonsPTC58-0592, Biology: BNCT Poster Discussion SessionsPTC58-0092, Poster AbstractsClinics: CNSPTC58-0302, Physics: Image GuidancePTC58-0464, General: New HorizonsPTC58-0352, Physics: Image GuidancePTC58-0465, General: New HorizonsPTC58-0476, Physics: Image GuidancePTC58-0100, General: New HorizonsPTC58-0235, Biology: Mathematical Modelling and SimulationPTC58-0349, Physics: Treatment PlanningPTC58-0094, Physics: 4D Treatment and Delivery Poster Discussion SessionsPTC58-0367, Physics: Dose Calculation and OptimisationPTC58-0400, Biology: Translational and BiomarkersPTC58-0244, Physics: Dose Calculation and OptimisationPTC58-0640, Biology: Mathematical Modelling and SimulationPTC58-0355, General: New Investigator SessionPTC58-0320, Physics: Quality Assurance and VerificationPTC58-0057, Physics: Quality Assurance and VerificationPTC58-0174, Physics: Quality Assurance and VerificationPTC58-0295, Physics: Dose Calculation and OptimisationPTC58-0529, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0123, Physics: Quality Assurance and VerificationPTC58-0171, Biology: Biological Differences between Carbon, Proton and Photons Poster Discussion SessionsPTC58-0049, Clinics: BreastPTC58-0731, General: New HorizonsPTC58-0223, General: New HorizonsPTC58-0102, General: New HorizonsPTC58-0466, Scientific: RTTPTC58-0503, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0389, General: New HorizonsPTC58-0108, General: New HorizonsPTC58-0109, Physics: Commissioning New FacilitiesPTC58-0736, Biology: Mathematical Modelling and SimulationPTC58-0343, Biology: Mathematical Modelling and SimulationPTC58-0342, Clinics: GI, GU, BreastPTC58-0237, Physics: Dose Calculation and OptimisationPTC58-0711, Biology: Mathematical Modelling and SimulationPTC58-0581, Clinics: GI, GU, BreastPTC58-0114, Clinics: Base of SkullPTC58-0730, Clinics: Head and neckPTC58-0383, Clinics: CNS / Skull BasePTC58-0559, Clinics: Base of SkullPTC58-0613, General: New HorizonsPTC58-0691, Biology: Biological Differences between Carbon, Proton and Photons Poster Discussion SessionsPTC58-0054, General: New HorizonsPTC58-0210, Clinics: BreastPTC58-0729, General: New HorizonsPTC58-0574, Clinics: GI, GU, BreastPTC58-0239, Scientific: RTTPTC58-0637, General: New HorizonsPTC58-0579, Clinics: Lung / Sarcoma / LymphomaPTC58-0176, General: New HorizonsPTC58-0699, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0156, Biology: Mathematical Modelling and SimulationPTC58-0333, Biology: Translational and BiomarkersPTC58-0345, Physics: Image GuidancePTC58-0369, Physics: Commissioning New FacilitiesPTC58-0509, Biology: Mathematical Modelling SimulationPTC58-0658, Biology: Biological Differences between Carbon, Proton and Photons Poster Discussion SessionsPTC58-0051, General: New Investigator SessionPTC58-0548, Clinics: GI, GU, BreastPTC58-0241, Clinics: Eye / Breast / Pelvis Poster Discussion SessionsPTC58-0412, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0024, Clinics: LungPTC58-0226, Biology: Biological Differences between Carbon, Proton and Photons Poster Discussion SessionsPTC58-0069, General: New HorizonsPTC58-0562, General: New HorizonsPTC58-0561, General: New HorizonsPTC58-0201, Biology: Mathematical Modelling and SimulationPTC58-0439, General: New HorizonsPTC58-0445, General: New HorizonsPTC58-0324, Physics: Image GuidancePTC58-0031, Biology: Mathematical Modelling and SimulationPTC58-0558, Physics: Image GuidancePTC58-0392, Biology: Mathematical Modelling and SimulationPTC58-0678, Physics: Beam Delivery and Nozzle DesignPTC58-0090, General: New Investigator SessionPTC58-0630, Biology: Biological Differences between Carbon / Proton and Photons Carbons / Proton and PhotonPTC58-0524, Physics: Commissioning New FacilitiesPTC58-0713, Clinics: GI, GU, BreastPTC58-0139, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0248, Clinics: CNS / Pediatrics / Lung Poster Discussion SessionsPTC58-0368, Biology: Enhanced Biology in Treatment PlanningPTC58-0519, General: New Horizons SessionPTC58-0720, Physics: Quality Assurance and VerificationPTC58-0083, General: New HorizonsPTC58-0311, General: New HorizonsPTC58-0674, General: New HorizonsPTC58-0553, Physics: Image GuidancePTC58-0023, Scientific: RTTPTC58-0612, General: New HorizonsPTC58-0677, Biology: Mathematical Modelling and SimulationPTC58-0545, Physics: Dose Calculation and OptimisationPTC58-0601, Physics: Dose Calculation and OptimisationPTC58-0725, Physics: Quality Assurance and VerificationPTC58-0098, Physics: Dose Calculation and OptimisationPTC58-0605, Biology: Biological Differences between Carbon / Proton and Photons Carbons / Proton and PhotonPTC58-0517, Biology: Translational and Biomarkers Poster Discussion SessionsPTC58-0618, Physics: Monitoring and Modelling MotionPTC58-0481, Clinics: GI / Sarcoma Poster Discussion SessionsPTC58-0071, Physics: Adaptive TherapyPTC58-0351, Physics: 4D Treatment and DeliveryPTC58-0702, Physics: Image GuidancePTC58-0734, Physics: Image GuidancePTC58-0611, Physics: Treatment Planning Poster Discussion SessionsPTC58-0486, Physics: Absolute and Relative Dosimetry Poster Discussion SessionsPTC58-0442, Biology: Drug and Immunotherapy CombinationsPTC58-0327, Clinics: Head and Neck / EyePTC58-0096, Clinics: LungPTC58-0159, Physics: Treatment PlanningPTC58-0708, General: New HorizonsPTC58-0097, Physics: Treatment Planning Poster Discussion SessionsPTC58-0350, Biology: Biological Differences between Carbon / Proton and Photons Carbons / Proton and PhotonPTC58-0016, Physics: Adaptive TherapyPTC58-0104, Physics: Absolute and Relative Dosimetry Poster Discussion SessionsPTC58-0433, Physics: Image GuidancePTC58-0608, Biology: Translational and Biomarkers Poster Discussion SessionsPTC58-0610, Clinics: Head and neckPTC58-0058, Physics: Treatment PlanningPTC58-0715, Clinics: Head and neckPTC58-0298, Clinics: EyePTC58-0099, General: New HorizonsPTC58-0086, General: New HorizonsPTC58-0089, Clinics: Lung / Sarcoma / LymphomaPTC58-0200, Poster AbstractsClinics: CNSPTC58-0157, Clinics: LungPTC58-0141, Clinics: LungPTC58-0260, Clinics: LungPTC58-0264, Physics: Image GuidancePTC58-0513, Physics: Image GuidancePTC58-0631, Clinics: Eye / Breast / Pelvis Poster Discussion SessionsPTC58-0469, Biology: BNCT Poster Discussion SessionsPTC58-0384, Physics: Image GuidancePTC58-0639, Clinics: PediatricsPTC58-0700, Clinics: LungPTC58-0136, Clinics: BreastPTC58-0706, General: New HorizonsPTC58-0079, Biology: Drug and Immunotherapy Combinations Poster Discussion SessionsPTC58-0406, Clinics: Base of SkullPTC58-0382, Physics: Image GuidancePTC58-0624, Physics: Beam Delivery and Nozzle DesignPTC58-0173, Biology: Drug and Immunotherapy CombinationsPTC58-0358, Poster AbstractsClinics: CNSPTC58-0690, General: New HorizonsPTC58-0061, Clinics: Lung / Sarcoma / LymphomaPTC58-0580, Physics: Monitoring and Modelling MotionPTC58-0162, Physics: Adaptive TherapyPTC58-0550, Physics: Adaptive TherapyPTC58-0430, Clinics: Lung / Sarcoma / LymphomaPTC58-0103, General: New Investigator SessionPTC58-0252, Physics: Quality Assurance and VerificationPTC58-0704, Physics: Image GuidancePTC58-0418, Clinics: Base of SkullPTC58-0572, Clinics: Lung / Sarcoma / LymphomaPTC58-0106, Physics: Beam Delivery and Nozzle DesignPTC58-0022, Physics: Monitoring and Modelling MotionPTC58-0279, Physics: Treatment Planning Poster Discussion SessionsPTC58-0447, Physics: Treatment PlanningPTC58-0622, Clinics: PediatricsPTC58-0644, Biology: Biology and Clinical InterfacePTC58-0490, Clinics: CNS / Skull BasePTC58-0716, General: New HorizonsPTC58-0292, Biology: Biological Differences between Carbon / Proton and Photons Carbons / Proton and PhotonPTC58-0570, General: New HorizonsPTC58-0059, Physics: Quality Assurance and VerificationPTC58-0710, Biology: Biological Differences between Carbon / Proton and Photons Carbons / Proton and PhotonPTC58-0216, Physics: Image GuidancePTC58-0404, Physics: Image GuidancePTC58-0525, Physics: Image GuidancePTC58-0526, Poster AbstractsClinics: CNSPTC58-0328, Clinics: LungPTC58-0070, Clinics: Eye / Breast / Pelvis Poster Discussion SessionsPTC58-0135, Biology: BNCT Poster Discussion SessionsPTC58-0391, Physics: Treatment PlanningPTC58-0510, Physics: Treatment PlanningPTC58-0636, Physics: Treatment PlanningPTC58-0638, Physics: Image GuidancePTC58-0408, Physics: Absolute and Relative Dosimetry Poster Discussion SessionsPTC58-0632, Physics: Treatment Planning Poster Discussion SessionsPTC58-0318, Biology: Enhanced Biology in Treatment PlanningPTC58-0246, Clinics: PediatricsPTC58-0504, General: New HorizonsPTC58-0160, Physics: Image Guidance Poster Discussion SessionsPTC58-0076, Physics: Monitoring and Modelling MotionPTC58-0143, Biology: Mathematical Modelling and SimulationPTC58-0718, Physics: Image GuidancePTC58-0671, Clinics: LungPTC58-0183, Physics: Image GuidancePTC58-0670, Report, Physics: Treatment Planning Poster Discussion SessionsPTC58-0422, Biology: Biological Differences between Carbon / Proton and Photons Carbons / Proton and PhotonPTC58-0129, Physics: Adaptive Therapy Poster Discussion SessionsPTC58-0705, Biology: Enhanced Biology in Treatment PlanningPTC58-0258, General: New HorizonsPTC58-0030, General: New HorizonsPTC58-0150, Biology: Biology and Clinical InterfacePTC58-0479, General: New HorizonsPTC58-0153, Clinics: PediatricPTC58-0087, General: New HorizonsPTC58-0152, General: New HorizonsPTC58-0155, General: New HorizonsPTC58-0033, General: New HorizonsPTC58-0158, Physics: Image GuidancePTC58-0429, Biology: Translational and BiomarkersPTC58-0287, Physics: Adaptive TherapyPTC58-0403, Physics: Image GuidancePTC58-0309
Published
2020

46. Advanced usage of Ti3C2Tx MXenes for photothermal therapy on different 3D breast cancer models

Author

Perini, Giordano, Rosenkranz, A., Friggeri, G., Zambrano, D., Rosa, Enrico, Augello, A., Palmieri, V., De Spirito, Marco, Papi, Massimiliano, Perini G. (ORCID:0000-0001-9452-8479), Rosa E., De Spirito M. (ORCID:0000-0003-4260-5107), Papi M. (ORCID:0000-0002-0029-1309), Perini, Giordano, Rosenkranz, A., Friggeri, G., Zambrano, D., Rosa, Enrico, Augello, A., Palmieri, V., De Spirito, Marco, Papi, Massimiliano, Perini G. (ORCID:0000-0001-9452-8479), Rosa E., De Spirito M. (ORCID:0000-0003-4260-5107), and Papi M. (ORCID:0000-0002-0029-1309)

Abstract

Globally, breast cancer is the most diagnosed invasive cancer among women. Current therapies (e.g., chemotherapy) show numerous limitations due to the lack of selectivity and involved side effects, which urgently asks for novel approaches with enhanced tumor-killing efficacy. We previously demonstrated that MXenes, new bioactive nanomaterials with promising photophysical properties, are capable to increase the efficiency of the targeted breast cancer photothermal therapy (PTT). In this work, we investigated the effect of few- and multi-layer Ti3C2Tx MXenes mediated-PTT on two different 3D reliable breast cancer models such as conventional and bio-printed spheroids. We performed PTT on both cancer models using a non-toxic MXene concentration of 50 µg/mL. After PTT, a significant reduction in the cell viability along with a notable increase in reactive oxygen species (ROS) was observed. Moreover, we studied the effect of PTT on the migration of macrophages and endothelial cells toward cancer regions in both 3D models. Our results indicate that PTT mediated by both few- and multi-layer MXenes significantly modulates the tumor progression through cells’ death by increasing the temperature, which holds particularly true for the bio-printed model.

Published
2022

47. Unsupervised Clustering of Heartbeat Dynamics Allows for Real Time and Personalized Improvement in Cardiovascular Fitness

Author

Serantoni, Cassandra, Zimatore, G., Bianchetti, Giada, Abeltino, Alessio, De Spirito, Marco, Maulucci, Giuseppe, Serantoni C., Bianchetti G., Abeltino A., De Spirito M. (ORCID:0000-0003-4260-5107), Maulucci G. (ORCID:0000-0002-2154-319X), Serantoni, Cassandra, Zimatore, G., Bianchetti, Giada, Abeltino, Alessio, De Spirito, Marco, Maulucci, Giuseppe, Serantoni C., Bianchetti G., Abeltino A., De Spirito M. (ORCID:0000-0003-4260-5107), and Maulucci G. (ORCID:0000-0002-2154-319X)

Abstract

VO2max index has a significant impact on overall health. Its estimation through wearables notifies the user of his level of fitness but cannot provide a detailed analysis of the time intervals in which heartbeat dynamics are changed and/or fatigue is emerging. Here, we developed a multiple modality biosignal processing method to investigate running sessions to characterize in real time heartbeat dynamics in response to external energy demand. We isolated dynamic regimes whose fraction increases with the VO2max and with the emergence of neuromuscular fatigue. This analysis can be extremely valuable by providing personalized feedback about the user's fitness level improvement that can be realized by developing personalized exercise plans aimed to target a contextual increase in the dynamic regime fraction related to VO2max increase, at the expense of the dynamic regime fraction related to the emergence of fatigue. These strategies can ultimately result in the reduction in cardiovascular risk.

Published
2022

48. Detecting Metabolic Thresholds from Nonlinear Analysis of Heart Rate Time Series: A Review

Author

Zimatore, G., Gallotta, M. C., Campanella, Massimo Giuseppe, Skarzynski, P. H., Maulucci, Giuseppe, Serantoni, Cassandra, De Spirito, Marco, Curzi, D., Guidetti, L., Baldari, C., Hatzopoulos, S., Campanella M., Maulucci G. (ORCID:0000-0002-2154-319X), Serantoni C., De Spirito M. (ORCID:0000-0003-4260-5107), Zimatore, G., Gallotta, M. C., Campanella, Massimo Giuseppe, Skarzynski, P. H., Maulucci, Giuseppe, Serantoni, Cassandra, De Spirito, Marco, Curzi, D., Guidetti, L., Baldari, C., Hatzopoulos, S., Campanella M., Maulucci G. (ORCID:0000-0002-2154-319X), Serantoni C., and De Spirito M. (ORCID:0000-0003-4260-5107)

Abstract

Heart rate time series are widely used to characterize physiological states and athletic performance. Among the main indicators of metabolic and physiological states, the detection of metabolic thresholds is an important tool in establishing training protocols in both sport and clinical fields. This paper reviews the most common methods, applied to heart rate (HR) time series, aiming to detect metabolic thresholds. These methodologies have been largely used to assess energy metabolism and to identify the appropriate intensity of physical exercise which can reduce body weight and improve physical fitness. Specifically, we focused on the main nonlinear signal evaluation methods using HR to identify metabolic thresholds with the purpose of identifying a method which can represent a useful tool for the real-time settings of wearable devices in sport activities. While the advantages and disadvantages of each method, and the possible applications, are presented, this review confirms that the nonlinear analysis of HR time series represents a solid, robust and noninvasive approach to assess metabolic thresholds.

Published
2022

49. Spatial Reorganization of Liquid Crystalline Domains of Red Blood Cells in Type 2 Diabetic Patients with Peripheral Artery Disease

Author

Bianchetti, Giada, Rizzo, Gaetano Emanuele, Serantoni, Cassandra, Abeltino, Alessio, Rizzi, A., Tartaglione, Linda, Caputo, Salvatore, Flex, Andrea, De Spirito, Marco, Pitocco, Dario, Maulucci, Giuseppe, Bianchetti G., Rizzo G. E., Serantoni C., Abeltino A., Tartaglione L., Caputo S. (ORCID:0000-0003-0772-6800), Flex A. (ORCID:0000-0003-2664-4165), De Spirito M. (ORCID:0000-0003-4260-5107), Pitocco D. (ORCID:0000-0002-6220-686X), Maulucci G. (ORCID:0000-0002-2154-319X), Bianchetti, Giada, Rizzo, Gaetano Emanuele, Serantoni, Cassandra, Abeltino, Alessio, Rizzi, A., Tartaglione, Linda, Caputo, Salvatore, Flex, Andrea, De Spirito, Marco, Pitocco, Dario, Maulucci, Giuseppe, Bianchetti G., Rizzo G. E., Serantoni C., Abeltino A., Tartaglione L., Caputo S. (ORCID:0000-0003-0772-6800), Flex A. (ORCID:0000-0003-2664-4165), De Spirito M. (ORCID:0000-0003-4260-5107), Pitocco D. (ORCID:0000-0002-6220-686X), and Maulucci G. (ORCID:0000-0002-2154-319X)

Abstract

In this work, we will investigate if red blood cell (RBC) membrane fluidity, influenced by several hyperglycemia-induced pathways, could provide a complementary index of HbA1c to monitor the development of type 2 diabetes mellitus (T2DM)-related macroangiopathic complications such as Peripheral Artery Disease (PAD). The contextual liquid crystalline (LC) domain spatial organization in the membrane was analysed to investigate the phase dynamics of the transition. Twenty-seven patients with long-duration T2DM were recruited and classified in DM, including 12 non-PAD patients, and DM + PAD, including 15 patients in any stage of PAD. Mean values of RBC generalized polarization (GP), representative of membrane fluidity, together with spatial organization of LC domains were compared between the two groups; p-values < 0.05 were considered statistically significant. Although comparable for anthropometric characteristics, duration of diabetes, and HbA1c, RBC membranes of PAD patients were found to be significantly more fluid (GP: 0.501 +/- 0.026) than non-PAD patients (GP: 0.519 +/- 0.007). These alterations were shown to be triggered by changes in both LC microdomain composition and distribution. We found a decrease in Feret diameter from 0.245 +/- 0.281 mu m in DM to 0.183 +/- 0.124 mu m in DM + PAD, and an increase in circularity. Altered RBC membrane fluidity is correlated to a spatial reconfiguration of LC domains, which, by possibly altering metabolic function, are associated with the development of T2DM-related macroangiopathic complications.

Published
2022

50. PTTG1/ZEB1 Axis Regulates E-Cadherin Expression in Human Seminoma

Author

Teveroni, Emanuela, Di Nicuolo, F., Vergani, Edoardo, Bianchetti, Giada, Bruno, C., Maulucci, Giuseppe, De Spirito, Marco, Cenci, Tonia, Pierconti, Francesco, Gulino, Gaetano, Bassi, Pierfrancesco, Pontecorvi, Alfredo, Milardi, D., Mancini, F., Teveroni E., Vergani E., Bianchetti G., Maulucci G. (ORCID:0000-0002-2154-319X), De Spirito M. (ORCID:0000-0003-4260-5107), Cenci T., Pierconti F. (ORCID:0000-0003-0951-4131), Gulino G., Bassi P. (ORCID:0000-0002-4313-8427), Pontecorvi A. (ORCID:0000-0003-0570-6865), Teveroni, Emanuela, Di Nicuolo, F., Vergani, Edoardo, Bianchetti, Giada, Bruno, C., Maulucci, Giuseppe, De Spirito, Marco, Cenci, Tonia, Pierconti, Francesco, Gulino, Gaetano, Bassi, Pierfrancesco, Pontecorvi, Alfredo, Milardi, D., Mancini, F., Teveroni E., Vergani E., Bianchetti G., Maulucci G. (ORCID:0000-0002-2154-319X), De Spirito M. (ORCID:0000-0003-4260-5107), Cenci T., Pierconti F. (ORCID:0000-0003-0951-4131), Gulino G., Bassi P. (ORCID:0000-0002-4313-8427), and Pontecorvi A. (ORCID:0000-0003-0570-6865)

Abstract

Simple Summary Seminoma represents one of the most common neoplasms in Caucasian males between 15 and 40 years old. The molecular pathways underlying its clinical behavior are far from being understood yet. We previously demonstrated that nuclear Pituitary-tumor transforming-gene 1 (PTTG1), overexpressed in several neoplasms, promotes invasiveness through its transcriptional target matrix-metalloproteinase-2 (MMP2). PTTG1 sustains the migratory and invasive properties of cancer cells through the induction of the epithelial-to-mesenchymal transition (EMT). E-Cadherin (E-CAD) repression is the first step of EMT. Therefore, we investigated the role of PTTG1 in EMT in human seminoma using an in vitro and in vivo model and through Atlas database interrogation. Our data showed a PTTG1-mediated E-CAD transcriptional repression through Zinc finger E-box binding homeobox 1 (ZEB1), a master regulator of the EMT process. Our data provide insights into the molecular characterization of seminoma, promoting PTTG1 as a prognostic marker useful in human seminoma clinical management. (1) Background: PTTG1 sustains the EMT process and the invasiveness of several neoplasms. We previously showed the role of nuclear PTTG1 in promoting invasiveness, through its transcriptional target MMP2, in seminoma in vitro models. Here, we investigated the key players involved in PTTG1-mediated EMT in human seminoma. (2) Methods: Two seminoma cell lines and four human seminoma tumor specimens were used. E-Cadherin gene regulation was investigated using Western blot, real-time PCR, and luciferase assay. Immunoprecipitation, ChIP, RE-ChIP, and confocal microscopy analysis were performed to evaluate the interplay between PTTG1 and ZEB1. Matrigel invasion and spheroid formation assays were applied to functionally investigate PTTG1 involvement in the EMT of seminoma cell lines. RNA depletion and overexpression experiments were performed to verify the role of PTTG1/ZEB1 in E-Cadherin repression and seminom

Published
2022

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