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3. An alphoid-like satellite DNA sequence is present in the genome of a lacertid lizard

Author

Ettore Olmo, M.G. De Santo, Gaetano Odierna, Teresa Capriglione, Capriglione, Teresa, DE SANTO, Mg, Odierna, Gaetano, and Olmo, E.

Subjects
Satellite DNA, Sequence analysis, Chromosomal Proteins, Non-Histone, Centromere, Molecular Sequence Data, DNA, Satellite, Genome, Autoantigens, Homology (biology), chemistry.chemical_compound, Lacertid lizard, PstI, Sequence Homology, Nucleic Acid, Genetics, Animals, Deoxyribonucleases, Type II Site-Specific, Molecular Biology, Ecology, Evolution, Behavior and Systematics, In Situ Hybridization, Repetitive Sequences, Nucleic Acid, biology, Base Sequence, Lizards, Sequence Analysis, DNA, DNA-Binding Proteins, genomic DNA, chemistry, biology.protein, Centromere Protein B, DNA
Abstract

A PstI DNA family was isolated from the genome of a lacertid, Lacerta graeca. The 185-bp monomeric unit (pGPS) was cloned and hybridized to DNAs and chromosomes of several lacertid species. The data showed that pGPS hybridizes to the (1) centromeric or pericentromeric heterochromatin of almost all the chromosomes of L. graeca and (2) genomic DNA of species phylogenetically related and unrelated to L. graeca. The presence of pGPS even in species immunologically apart more than 30 million years suggests that this repeated family might be either very ancient or have been conserved during evolution due to its functional role. The latter hypothesis might be supported by the results of sequence analysis which showed some homology with both several alphoid sequences of primates and the CDEIII centromeric sequence of yeast. Segments of the satellite sequence are similar to the mammalian CENP-B box. These observations suggest that pGPS might have a role in determining the centromeric function in lacertid lizards.

Published
1998

4. An alphoid-like satellite DNA sequence is present in the genome of a lacertid lizard.

Author

Capriglione T, De Santo MG, Odierna G, and Olmo E

Subjects
Animals, Base Sequence, Centromere genetics, Centromere Protein B, Chromosomal Proteins, Non-Histone genetics, Deoxyribonucleases, Type II Site-Specific genetics, In Situ Hybridization, Molecular Sequence Data, Repetitive Sequences, Nucleic Acid, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Autoantigens, DNA, Satellite, DNA-Binding Proteins, Lizards genetics
Abstract

A PstI DNA family was isolated from the genome of a lacertid, Lacerta graeca. The 185-bp monomeric unit (pGPS) was cloned and hybridized to DNAs and chromosomes of several lacertid species. The data showed that pGPS hybridizes to the (1) centromeric or pericentromeric heterochromatin of almost all the chromosomes of L. graeca and (2) genomic DNA of species phylogenetically related and unrelated to L. graeca. The presence of pGPS even in species immunologically apart more than 30 million years suggests that this repeated family might be either very ancient or have been conserved during evolution due to its functional role. The latter hypothesis might be supported by the results of sequence analysis which showed some homology with both several alphoid sequences of primates and the CDEIII centromeric sequence of yeast. Segments of the satellite sequence are similar to the mammalian CENP-B box. These observations suggest that pGPS might have a role in determining the centromeric function in lacertid lizards.

Published
1998
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