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133 results on '"De Rossi, Edda"'

2. Benzothiazinones Kill Mycobacterium tuberculosis by Blocking Arabinan Synthesis

Author

Makarov, Vadim, Manina, Giulia, Mikusova, Katarina, Möllmann, Ute, Ryabova, Olga, Saint-Joanis, Brigitte, Dhar, Neeraj, Pasca, Maria Rosalia, Buroni, Silvia, Lucarelli, Anna Paola, Milano, Anna, De Rossi, Edda, Belanova, Martina, Bobovska, Adela, Dianiskova, Petronela, Kordulakova, Jana, Sala, Claudia, Fullam, Elizabeth, Schneider, Patricia, McKinney, John D., Brodin, Priscille, Christophe, Thierry, Waddell, Simon, Butcher, Philip, Albrethsen, Jakob, Rosenkrands, Ida, Brosch, Roland, Nandi, Vrinda, Bharath, Sowmya, Gaonkar, Sheshagiri, Shandil, Radha K., Balasubramanian, Venkataraman, Balganesh, Tanjore, Tyagi, Sandeep, Grosset, Jacques, Riccardi, Giovanna, and Cole, Stewart T.

Published
2009
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3. Structural plasticity and distinct drug-binding modes of LfrR, a mycobacterial efflux pump regulator

Author

Bellinzoni, Marco, Buroni, Silvia, Schaeffer, Francis, Riccardi, Giovanna, De Rossi, Edda, and Alzari, Pedro M.

Subjects
Drug resistance in microorganisms -- Research, Mycobacteria -- Physiological aspects, Mycobacterium -- Physiological aspects, Adaptation (Physiology) -- Research, Biological sciences
Abstract

The TetR-like transcriptional repressor LfrR controls the expression of the gene encoding the Mycobacterium smegmatis efflux pump LfrA, which actively extrudes fluoroquinolones, cationic dyes, and anthracyclines from the cell and promotes intrinsic antibiotic resistance. The crystal structure of the apoprotein form of the repressor reveals a structurally asymmetric homodimer exhibiting local unfolding and a blocked drug-binding site, emphasizing the significant conformational plasticity of the protein necessary for DNA and multidrug recognition. Crystallographic and calorimetric studies of LfrR-drug complexes further confirm the intrinsic flexibility of the homodimer, which provides a dynamic mechanism to broaden multidrug binding specificity and may be a general property of transcriptional repressors regulating microbial efflux pump expression. doi: 10.1128/JB.00631-09

Published
2009

4. Antistaphylococcal Activity of the FtsZ Inhibitor C109

Author

Trespidi, Gabriele, primary, Scoffone, Viola, additional, Barbieri, Giulia, additional, Marchesini, Federica, additional, Abualshaar, Aseel, additional, Coenye, Tom, additional, Ungaro, Francesca, additional, Makarov, Vadim, additional, Migliavacca, Roberta, additional, De Rossi, Edda, additional, and Buroni, Silvia, additional

Published
2021
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8. Biological and structural characterization of the Mycobacterium smegmatis nitroreductase NfnB, and its role in benzothiazinone resistance

Author

Manina, Giulia, Bellinzoni, Marco, Pasca, Maria Rosalia, Neres, João, Milano, Anna, de Jesus Lopes Ribeiro, Ana Luisa, Buroni, Silvia, Škovierová, Henrieta, Dianišková, Petronela, Mikušová, Katarína, Marák, Jozef, Makarov, Vadim, Giganti, David, Haouz, Ahmed, Lucarelli, Anna Paola, Degiacomi, Giulia, Piazza, Aurora, Chiarelli, Laurent R., De Rossi, Edda, Salina, Elena, Cole, Stewart T., Alzari, Pedro M., and Riccardi, Giovanna

Published
2010
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11. Determination of a 15447 bp nucleotide sequence around the inhA gene of Mycobacterium avium and similarity analysis of the products of putative ORFs

Author

Labo, Monica, Gusberti, Laura, De Rossi, Edda, Speziale, Pietro, and Ricardi, Giovanna

Subjects
Mycobacterium avium -- Physiological aspects, Nucleotide sequence -- Observations, Biological sciences
Abstract

The cloning and sequencing of a 15437 bp region incorporating the inhA locus from the Mycobacterium avium chromosome was undertaken. The region ws found to contain acnA, fabG, inhA and hemH genes, and invA and invB genes showing homology with p60 invasion protein of Listeria monocytogenes. An RGD motif was contained in six proteins encoded by putative ORFs, with other putative products encoded by ORFs showing little homology with proteins included in the databases.

Published
1998

13. Gene arrangement and organization in an approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae

Author

Fsihi, Hafida, De Rossi, Edda, Riccardi, Giovanna, Takiff, Howard E., Eiglmeier, Karin, Bergh, Staffan, and Cole, Stewart T.

Subjects
Mycobacterium leprae -- Genetic aspects, Genomes -- Analysis, Chromosome mapping -- Analysis, Molecular genetics -- Research, Biological sciences
Abstract

Nucleotide sequencing of the 75 627 bp segment of the Mycobacterium leprae chromosome with the oriC gene indicated the presence of a gene order that is similar to the replication origin region of prokaryotes. The 75 627 bp segment also contains gyrB, dnaA, dnaB and gidB genes which mediate deoxyribonucleotide metabolism, cell wall synthesis and cell division. Furthermore, the protein products of the open reading frames that are located in the region mediate the generation of serine/threonine protein kinases and protein phosphatase.

Published
1996

17. Molecular cloning and sequencing of the beta-isopropylmalate dehydrogenase gene from the cyanobacterium Spirulina platensis

Author

Bini, Francesca, De Rossi, Edda, Barbierato, Laura, and Riccardi, Giovanna

Subjects
Spirulina -- Research, Dehydrogenases -- Genetic aspects, Leucine -- Genetic aspects, Biological sciences
Abstract

The beta-isopropylmalate dehydrogenase gene of Spirulina platensis was cloned by heterologous hybridization. The enzyme is a component of the isopropylpalmitate pathway used by photoautotrophic organisms for leucine synthesis. DNA sequencing and transcriptional analysis done using the dideoxy chain-termination method revealed an open reading frame of 1,065 nucleotides encoding a protein of 355 amino acids with an estimated molecular mass of 39,000 daltons.

Published
1992

22. Efflux pump genes of the resistance-nodulation-division family in Burkholderia cenocepacia genome

Author

Manina Giulia, Arrigo Patrizio, Buroni Silvia, De Rossi Edda, Pasca Maria, Guglierame Paola, and Riccardi Giovanna

Subjects
Microbiology, QR1-502
Abstract

Abstract Background Burkholderia cenocepacia is recognized as opportunistic pathogen that can cause lung infections in cystic fibrosis patients. A hallmark of B. cenocepacia infections is the inability to eradicate the organism because of multiple intrinsic antibiotic resistance. As Resistance-Nodulation-Division (RND) efflux systems are responsible for much of the intrinsic multidrug resistance in Gram-negative bacteria, this study aims to identify RND genes in the B. cenocepacia genome and start to investigate their involvement into antimicrobial resistance. Results Genome analysis and homology searches revealed 14 open reading frames encoding putative drug efflux pumps belonging to RND family in B. cenocepacia J2315 strain. By reverse transcription (RT)-PCR analysis, it was found that orf3, orf9, orf11, and orf13 were expressed at detectable levels, while orf10 appeared to be weakly expressed in B. cenocepacia. Futhermore, orf3 was strongly induced by chloramphenicol. The orf2 conferred resistance to fluoroquinolones, tetraphenylphosphonium, streptomycin, and ethidium bromide when cloned and expressed in Escherichia coli KAM3, a strain lacking the multidrug efflux pump AcrAB. The orf2-overexpressing E. coli also accumulate low concentrations of ethidium bromide, which was restored to wild type level in the presence of CCCP, an energy uncoupler altering the energy of the drug efflux pump. Conclusion The 14 RND pumps gene we have identified in the genome of B. cenocepacia suggest that active efflux could be a major mechanism underlying antimicrobial resistance in this microorganism. We have characterized the ORF2 pump, one of these 14 potential RND efflux systems. Its overexpression in E. coli conferred resistance to several antibiotics and to ethidium bromide but it remains to be determined if this pump play a significant role in the antimicrobial intrinsic resistance of B. cenocepacia. The characterization of antibiotic efflux pumps in B. cenocepacia is an obligatory step prior to the design of specific, potent bacterial inhibitors for the improved control of infectious diseases. Consequently, the topic deserves to be further investigated and future studies will involve systematic investigation on the function and expression of each of the RND efflux pump homologs.

Published
2006
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23. Investigating the Mechanism of Action of Diketopiperazines Inhibitors of the Burkholderia cenocepacia Quorum Sensing Synthase CepI: A Site-Directed Mutagenesis Study

Author

Buroni, Silvia, primary, Scoffone, Viola C., additional, Fumagalli, Marco, additional, Makarov, Vadim, additional, Cagnone, Maddalena, additional, Trespidi, Gabriele, additional, De Rossi, Edda, additional, Forneris, Federico, additional, Riccardi, Giovanna, additional, and Chiarelli, Laurent R., additional

Published
2018
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27. 2-Carboxyquinoxalines Kill Mycobacterium tuberculosis through Noncovalent Inhibition of DprE1

Author

Neres, João, primary, Hartkoorn, Ruben C., additional, Chiarelli, Laurent R., additional, Gadupudi, Ramakrishna, additional, Pasca, Maria Rosalia, additional, Mori, Giorgia, additional, Venturelli, Alberto, additional, Savina, Svetlana, additional, Makarov, Vadim, additional, Kolly, Gaelle S., additional, Molteni, Elisabetta, additional, Binda, Claudia, additional, Dhar, Neeraj, additional, Ferrari, Stefania, additional, Brodin, Priscille, additional, Delorme, Vincent, additional, Landry, Valérie, additional, de Jesus Lopes Ribeiro, Ana Luisa, additional, Farina, Davide, additional, Saxena, Puneet, additional, Pojer, Florence, additional, Carta, Antonio, additional, Luciani, Rosaria, additional, Porta, Alessio, additional, Zanoni, Giuseppe, additional, De Rossi, Edda, additional, Costi, Maria Paola, additional, Riccardi, Giovanna, additional, and Cole, Stewart T., additional

Published
2014
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30. Improved BM212 MmpL3 Inhibitor Analogue Shows Efficacy in Acute Murine Model of Tuberculosis Infection

Author

Poce, Giovanna, primary, Bates, Robert H., additional, Alfonso, Salvatore, additional, Cocozza, Martina, additional, Porretta, Giulio Cesare, additional, Ballell, Lluís, additional, Rullas, Joaquin, additional, Ortega, Fátima, additional, De Logu, Alessandro, additional, Agus, Emanuela, additional, La Rosa, Valentina, additional, Pasca, Maria Rosalia, additional, De Rossi, Edda, additional, Wae, Baojie, additional, Franzblau, Scott G., additional, Manetti, Fabrizio, additional, Botta, Maurizio, additional, and Biava, Mariangela, additional

Published
2013
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31. Structural Basis for Benzothiazinone-Mediated Killing of Mycobacterium tuberculosis

Author

Neres, João, primary, Pojer, Florence, additional, Molteni, Elisabetta, additional, Chiarelli, Laurent R., additional, Dhar, Neeraj, additional, Boy-Röttger, Stefanie, additional, Buroni, Silvia, additional, Fullam, Elizabeth, additional, Degiacomi, Giulia, additional, Lucarelli, Anna Paola, additional, Read, Randy J., additional, Zanoni, Giuseppe, additional, Edmondson, Dale E., additional, De Rossi, Edda, additional, Pasca, Maria Rosalia, additional, McKinney, John D., additional, Dyson, Paul J., additional, Riccardi, Giovanna, additional, Mattevi, Andrea, additional, Cole, Stewart T., additional, and Binda, Claudia, additional

Published
2012
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33. MmpL3 Is the Cellular Target of the Antitubercular Pyrrole Derivative BM212

Author

La Rosa, Valentina, primary, Poce, Giovanna, additional, Canseco, Julio Ortiz, additional, Buroni, Silvia, additional, Pasca, Maria Rosalia, additional, Biava, Mariangela, additional, Raju, Ravikiran M., additional, Porretta, Giulio Cesare, additional, Alfonso, Salvatore, additional, Battilocchio, Claudio, additional, Javid, Babak, additional, Sorrentino, Flavia, additional, Ioerger, Thomas R., additional, Sacchettini, James C., additional, Manetti, Fabrizio, additional, Botta, Maurizio, additional, De Logu, Alessandro, additional, Rubin, Eric J., additional, and De Rossi, Edda, additional

Published
2012
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34. Art, biology and conservation 2002 : biodeterioration of works of art : a symposium

Author

De Montebello, Philippe, Frantz, James H., Koestler, Robert J. (Robert John), 1950, Charola, A. Elena, Jones, Mark, Berovic, M. (Marin), Strzelczyk, Alicja, Rosa, Halina, Erhardt, David, Tumosa, Charles, VonEndt, David W., Eveleigh, Douglas E., Arai, Hideo, Baldwin, Ann, Bonaventura, Maria, Centeno, Silvia, Wypyski, M. T., Szczepanowska, Hanna M., Cavaliere, Ralph, Van Dyke, Yana, Ciferri, Orio, 1928, Florian, Mary-Lou E., De Rossi, Edda, Becker, Mary, Ballard, Mary W., Peacock, Elizabeth E., Gonzalez, Juan M., Laiz, Leonila, Saiz-Jimenez, C. (Cesareo), Zanardini, Elisabetta, Abbruscato, Pamela, Scaramelli, Laura, Onelli, Elisabetta, Realini, Marco, Patrignani, Giuseppe, Sorlini, Claudia, Salvadori, O., Mitchell, Ralph, 1934, Karbowska-Berent, Joanna, Wessel, David P., Metropolitan Museum of Art (New York, N.Y.), De Montebello, Philippe, Frantz, James H., Koestler, Robert J. (Robert John), 1950, Charola, A. Elena, Jones, Mark, Berovic, M. (Marin), Strzelczyk, Alicja, Rosa, Halina, Erhardt, David, Tumosa, Charles, VonEndt, David W., Eveleigh, Douglas E., Arai, Hideo, Baldwin, Ann, Bonaventura, Maria, Centeno, Silvia, Wypyski, M. T., Szczepanowska, Hanna M., Cavaliere, Ralph, Van Dyke, Yana, Ciferri, Orio, 1928, Florian, Mary-Lou E., De Rossi, Edda, Becker, Mary, Ballard, Mary W., Peacock, Elizabeth E., Gonzalez, Juan M., Laiz, Leonila, Saiz-Jimenez, C. (Cesareo), Zanardini, Elisabetta, Abbruscato, Pamela, Scaramelli, Laura, Onelli, Elisabetta, Realini, Marco, Patrignani, Giuseppe, Sorlini, Claudia, Salvadori, O., Mitchell, Ralph, 1934, Karbowska-Berent, Joanna, Wessel, David P., and Metropolitan Museum of Art (New York, N.Y.)

Abstract

Brochure for a symposium held June 13-15, 2002 at The Metropolitan Museum of Art

Published
2002

47. 2-Carboxyquinoxalines Kill Mycobacterium tuberculosisthrough Noncovalent Inhibition of DprE1

Author

Neres, João, Hartkoorn, Ruben C., Chiarelli, Laurent R., Gadupudi, Ramakrishna, Pasca, Maria Rosalia, Mori, Giorgia, Venturelli, Alberto, Savina, Svetlana, Makarov, Vadim, Kolly, Gaelle S., Molteni, Elisabetta, Binda, Claudia, Dhar, Neeraj, Ferrari, Stefania, Brodin, Priscille, Delorme, Vincent, Landry, Valérie, de Jesus Lopes Ribeiro, Ana Luisa, Farina, Davide, Saxena, Puneet, Pojer, Florence, Carta, Antonio, Luciani, Rosaria, Porta, Alessio, Zanoni, Giuseppe, De Rossi, Edda, Costi, Maria Paola, Riccardi, Giovanna, and Cole, Stewart T.

Abstract

Phenotypic screening of a quinoxaline library against replicating Mycobacterium tuberculosisled to the identification of lead compound Ty38c (3-((4-methoxybenzyl)amino)-6-(trifluoromethyl)quinoxaline-2-carboxylic acid). With an MIC99and MBC of 3.1 μM, Ty38c is bactericidal and active against intracellular bacteria. To investigate its mechanism of action, we isolated mutants resistant to Ty38c and sequenced their genomes. Mutations were found in rv3405c, coding for the transcriptional repressor of the divergently expressed rv3406gene. Biochemical studies clearly showed that Rv3406 decarboxylates Ty38c into its inactive keto metabolite. The actual target was then identified by isolating Ty38c-resistant mutants of an M. tuberculosisstrain lacking rv3406. Here, mutations were found in dprE1, encoding the decaprenylphosphoryl-d-ribose oxidase DprE1, essential for biogenesis of the mycobacterial cell wall. Genetics, biochemical validation, and X-ray crystallography revealed Ty38c to be a noncovalent, noncompetitive DprE1 inhibitor. Structure–activity relationship studies generated a family of DprE1 inhibitors with a range of IC50’s and bactericidal activity. Co-crystal structures of DprE1 in complex with eight different quinoxaline analogs provided a high-resolution interaction map of the active site of this extremely vulnerable target in M. tuberculosis.

Published
2015
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48. MmpL3 Is the Cellular Target of the Antitubercular Pyrrole Derivative BM212

Author

La Rosa, Valentina, Poce, Giovanna, Canseco, Julio Ortiz, Buroni, Silvia, Pasca, Maria Rosalia, Biava, Mariangela, Raju, Ravikiran M., Porretta, Giulio Cesare, Alfonso, Salvatore, Battilocchio, Claudio, Javid, Babak, Sorrentino, Flavia, Ioerger, Thomas R., Sacchettini, James C., Manetti, Fabrizio, Botta, Maurizio, De Logu, Alessandro, Rubin, Eric J., and De Rossi, Edda

Abstract

ABSTRACTThe 1,5-diarylpyrrole derivative BM212 was previously shown to be active against multidrug-resistant clinical isolates and Mycobacterium tuberculosisresiding within macrophages as well as against Mycobacterium aviumand other atypical mycobacteria. To determine its mechanism of action, we identified the cellular target. Spontaneous Mycobacterium smegmatis, Mycobacterium bovisBCG, and M. tuberculosisH37Rv mutants that were resistant to BM212 were isolated. By the screening of genomic libraries and by whole-genome sequencing, we found that all the characterized mutants showed mutations in the mmpL3gene, allowing us to conclude that resistance to BM212 maps to the MmpL3 protein, a member of the MmpL (mycobacterial membrane protein, large) family. Susceptibility was unaffected by the efflux pump inhibitors reserpine, carbonylcyanide m-chlorophenylhydrazone, and verapamil. Uptake/efflux experiments with [14C]BM212 demonstrated that resistance is not driven by the efflux of BM212. Together, these data strongly suggest that the MmpL3 protein is the cellular target of BM212.

Published
2011
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49. Gene arrangement and organization in a ...76 kb fragment encompassing the oriC region of...

Author

Fsihi, Hafida, De Rossi, Edda, Salazar, Leiria, Cantoni, Rita, Labo, Monica, Riccardi, Giovanna, Takiff, Howard E., Eiglmeier, Karin, Bergh, Staffan, and Cole, Stewart T.

Subjects
*MYCOBACTERIUM leprae, *BACTERIAL chromosomes
Abstract

Analyzes the gene arrangement and organization in a difference 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium (M.) leprae. Description of M. leprae; Identification of genes and proteins involved in DNA replication and metabolism; Implications for cell division and genome evolution.

Published
1996
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50. Molecular Cloning and Functional Analysis of a Novel Tetracycline Resistance Determinant, tet(V), fromMycobacterium smegmatis

Author

De Rossi, Edda, Blokpoel, Marian C. J., Cantoni, Rita, Branzoni, Manuela, Riccardi, Giovanna, Young, Douglas B., De Smet, Koen A. L., and Ciferri, Orio

Abstract

ABSTRACTThe nucleotide sequence and mechanism of action of a tetracycline resistance gene from Mycobacterium smegmatiswere determined. Analysis of a 2.2-kb sequence fragment showed the presence of one open reading frame, designated tet(V), encoding a 419-amino-acid protein (molecular weight, 44,610) with at least 10 transmembrane domains. A database search showed that the gene is homologous to membrane-associated antibiotic efflux pump proteins but not to any known tetracycline efflux pumps. The steady-state accumulation level of tetracycline by M. smegmatisharboring a plasmid carrying the tet(V) gene was about fourfold lower than that of the parental strain. Furthermore, the energy uncoupler carbonyl cyanide m-chlorophenylhydrazone blocked tetracycline efflux in deenergized cells. These results suggest that the tet(V) gene codes for a drug antiporter which uses the proton motive force for the active efflux of tetracycline. By primer-specific amplification the gene appears to be restricted toM. smegmatisand M. fortuitum.

Published
1998
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