Search

Your search keyword '"De Rond, Leonie"' showing total 16 results
Start Over Author "De Rond, Leonie"
16 results on '"De Rond, Leonie"'

2. A compendium of single extracellular vesicle flow cytometry

Author

Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, van der Pol, Edwin, Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, and van der Pol, Edwin

Abstract

Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs. Whereas cells exceed the background noise, signals originating from EVs partly overlap with the background noise, thereby making EVs more difficult to detect than cells. This technical mismatch together with complexity of EV-containing fluids causes limitations and challenges with conducting, interpreting and reproducing EV FCM experiments. To address and overcome these challenges, researchers from the International Society for Extracellular Vesicles (ISEV), International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH) joined forces and initiated the EV FCM working group. To improve the interpretation, reporting, and reproducibility of future EV FCM data, the EV FCM working group published an ISEV position manuscript outlining a framework of minimum information that should be reported about an FCM experiment on single EVs (MIFlowCyt-EV). However, the framework contains limited background information. Therefore, the goal of this compendium is to provide the background information necessary to design and conduct reproducible EV FCM experiments. This compendium contains background information on EVs, the interaction between light and EVs, FCM hardware, experimental design and preanalytical procedures, sample preparation, assay controls, instrument data acquisition and calibration, EV characterization, and data reporting. Although this compendium focuses on EVs, many concepts and explanations could also be applied to FCM detection of other particles within the EV size range, such as bacteria, lipoprotein particles, milk fat globules, and viruses.

Published
2023

3. A compendium of single extracellular vesicle flow cytometry

Author

IOV Facs, Celbiologie, Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, van der Pol, Edwin, IOV Facs, Celbiologie, Welsh, Joshua A, Arkesteijn, Ger J A, Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G, Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A, Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Nieuwland, Rienk, and van der Pol, Edwin

Published
2023

4. A compendium of single extracellular vesicle flow cytometry

Author

Welsh, Joshua A., primary, Arkesteijn, Ger J. A., additional, Bremer, Michel, additional, Cimorelli, Michael, additional, Dignat‐George, Françoise, additional, Giebel, Bernd, additional, Görgens, André, additional, Hendrix, An, additional, Kuiper, Martine, additional, Lacroix, Romaric, additional, Lannigan, Joanne, additional, van Leeuwen, Ton G., additional, Lozano‐Andrés, Estefanía, additional, Rao, Shoaib, additional, Robert, Stéphane, additional, de Rond, Leonie, additional, Tang, Vera A., additional, Tertel, Tobias, additional, Yan, Xiaomei, additional, Wauben, Marca H. M., additional, Nolan, John P., additional, Jones, Jennifer C., additional, Nieuwland, Rienk, additional, and van der Pol, Edwin, additional

Published
2023
Full Text
View/download PDF

5. A compendium of single extracellular vesicle flow cytometry

Author

Welsh, Joshua A., Arkesteijn, Ger J. A., Bremer, Michel, Cimorelli, Michael, Dignat-George, Françoise, Giebel, Bernd, Görgens, André, Hendrix, An, Kuiper, Martine, Lacroix, Romaric, Lannigan, Joanne, van Leeuwen, Ton G., Lozano-Andrés, Estefanía, Rao, Shoaib, Robert, Stéphane, de Rond, Leonie, Tang, Vera A., Tertel, Tobias, Yan, Xiaomei, Wauben, Marca H. M., Nolan, John P., Jones, Jennifer C., Nieuwland, Rienk, and van der Pol, Edwin

Subjects
Medizin
Abstract

Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs. Whereas cells exceed the background noise, signals originating from EVs partly overlap with the background noise, thereby making EVs more difficult to detect than cells. This technical mismatch together with complexity of EV-containing fluids causes limitations and challenges with conducting, interpreting and reproducing EV FCM experiments. To address and overcome these challenges, researchers from the International Society for Extracellular Vesicles (ISEV), International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH) joined forces and initiated the EV FCM working group. To improve the interpretation, reporting, and reproducibility of future EV FCM data, the EV FCM working group published an ISEV position manuscript outlining a framework of minimum information that should be reported about an FCM experiment on single EVs (MIFlowCyt-EV). However, the framework contains limited background information. Therefore, the goal of this compendium is to provide the background information necessary to design and conduct reproducible EV FCM experiments. This compendium contains background information on EVs, the interaction between light and EVs, FCM hardware, experimental design and preanalytical procedures, sample preparation, assay controls, instrument data acquisition and calibration, EV characterization, and data reporting. Although this compendium focuses on EVs, many concepts and explanations could also be applied to FCM detection of other particles within the EV size range, such as bacteria, lipoprotein particles, milk fat globules, and viruses. CA extern

Published
2023

6. Gently down the stream: Detection of extracellular vesicles by flow cytometry

Author

de Rond, Leonie, van Leeuwen, A.G.J.M., Sturk, A., Coumans, F.A.W., Nieuwland, R., Faculteit der Geneeskunde, van Leeuwen, Ton G. J. M., Sturk, Guus, Coumans, Frank A. W., Nieuwland, Rienk, Biomedical Engineering and Physics, ACS - Atherosclerosis & ischemic syndromes, ACS - Microcirculation, and CCA - Imaging and biomarkers

Abstract

Cells release extracellular vesicles (EVs) into their environment for protection, waste removal, and intercellular communication. As a result, body fluids contain EVs, which are particles with a phospholipid membrane that range in diameter from 30 to 1,000 nm. The molecular composition of an EV depends on the origin and status (e.g. resting, activated, apoptotic) of the releasing cell. The concentration and composition of EVs in body fluids therefore changes with disease, making EVs potential biomarkers. To use EVs as biomarker, there is a need for a technique suitable for clinical application that can 1) detect particles as small as EVs, 2) distinguish EVs from non-EV particles, 3) identify the cellular origin of single EVs, and 4) measure the concentration of EVs in a way that allows comparison between instruments and institutes. Flow cytometry has a high potential to fulfil these requirements. The aim of this thesis is to improve the detection and characterization of single EVs by flow cytometry, to eventually allow the use of EVs as biomarkers in a clinical setting. To that end, this thesis includes methods to 1) lower the scatter detection limit for EVs of a commercial flow cytometer, 2) distinguish EVs from non-EV particles using their refractive index, and 3) compare data from different flow cytometers by applying a calibration method. Scatter detection in flow cytometry is thereby ready to study the biomarker potential of EVs in a clinical setting. Less

Published
2020

7. MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Author

Welsh, Joshua A, Van Der Pol, Edwin, Arkesteijn, Ger J A, Bremer, Michel, Brisson, Alain, Coumans, Frank, Dignat-George, Françoise, Duggan, Erika, Ghiran, Ionita, Giebel, Bernd, Görgens, André, Hendrix, An, Lacroix, Romaric, Lannigan, Joanne, Libregts, Sten F W M, Lozano-Andrés, Estefanía, Morales-Kastresana, Aizea, Robert, Stephane, De Rond, Leonie, Tertel, Tobias, Tigges, John, De Wever, Olivier, Yan, Xiaomei, Nieuwland, Rienk, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, Welsh, Joshua A, Van Der Pol, Edwin, Arkesteijn, Ger J A, Bremer, Michel, Brisson, Alain, Coumans, Frank, Dignat-George, Françoise, Duggan, Erika, Ghiran, Ionita, Giebel, Bernd, Görgens, André, Hendrix, An, Lacroix, Romaric, Lannigan, Joanne, Libregts, Sten F W M, Lozano-Andrés, Estefanía, Morales-Kastresana, Aizea, Robert, Stephane, De Rond, Leonie, Tertel, Tobias, Tigges, John, De Wever, Olivier, Yan, Xiaomei, Nieuwland, Rienk, Wauben, Marca H M, Nolan, John P, and Jones, Jennifer C

Abstract

Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field's lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental design and lack appropriate calibration and standardization. To address these issues, a working group (WG) of EV-FC researchers from ISEV, ISAC and ISTH, worked together as an EV-FC WG and developed a consensus framework for the minimum information that should be provided regarding EV-FC. This framework incorporates the existing Minimum Information for Studies of EVs (MISEV) guidelines and Minimum Information about a FC experiment (MIFlowCyt) standard in an EV-FC-specific reporting framework (MIFlowCyt-EV) that supports reporting of critical information related to sample staining, EV detection and measurement and experimental design in manuscripts that report EV-FC data. MIFlowCyt-EV provides a structure for sharing EV-FC results, but it does not prescribe specific protocols, as there will continue to be rapid evolution of instruments and methods for the foreseeable future. MIFlowCyt-EV accommodates this evolution, while providing information needed to evaluate and compare different approaches. Because MIFlowCyt-EV will ensure consistency in the manner of reporting of EV-FC studies, over time we expect that adoption of MIFlowCyt-EV as a standard for reporting EV- FC studies will improve the ability to quantitatively compare results from different laboratori

Published
2020

8. MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Author

LS Celbiologie-Algemeen, Celbiologie, dB&C I&I, Welsh, Joshua A, Van Der Pol, Edwin, Arkesteijn, Ger J A, Bremer, Michel, Brisson, Alain, Coumans, Frank, Dignat-George, Françoise, Duggan, Erika, Ghiran, Ionita, Giebel, Bernd, Görgens, André, Hendrix, An, Lacroix, Romaric, Lannigan, Joanne, Libregts, Sten F W M, Lozano-Andrés, Estefanía, Morales-Kastresana, Aizea, Robert, Stephane, De Rond, Leonie, Tertel, Tobias, Tigges, John, De Wever, Olivier, Yan, Xiaomei, Nieuwland, Rienk, Wauben, Marca H M, Nolan, John P, Jones, Jennifer C, LS Celbiologie-Algemeen, Celbiologie, dB&C I&I, Welsh, Joshua A, Van Der Pol, Edwin, Arkesteijn, Ger J A, Bremer, Michel, Brisson, Alain, Coumans, Frank, Dignat-George, Françoise, Duggan, Erika, Ghiran, Ionita, Giebel, Bernd, Görgens, André, Hendrix, An, Lacroix, Romaric, Lannigan, Joanne, Libregts, Sten F W M, Lozano-Andrés, Estefanía, Morales-Kastresana, Aizea, Robert, Stephane, De Rond, Leonie, Tertel, Tobias, Tigges, John, De Wever, Olivier, Yan, Xiaomei, Nieuwland, Rienk, Wauben, Marca H M, Nolan, John P, and Jones, Jennifer C

Published
2020

10. MIFlowCyt‐EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Author

Welsh, Joshua A., primary, Van Der Pol, Edwin, additional, Arkesteijn, Ger J.A., additional, Bremer, Michel, additional, Brisson, Alain, additional, Coumans, Frank, additional, Dignat‐George, Françoise, additional, Duggan, Erika, additional, Ghiran, Ionita, additional, Giebel, Bernd, additional, Görgens, André, additional, Hendrix, An, additional, Lacroix, Romaric, additional, Lannigan, Joanne, additional, Libregts, Sten F.W.M., additional, Lozano‐Andrés, Estefanía, additional, Morales‐Kastresana, Aizea, additional, Robert, Stephane, additional, De Rond, Leonie, additional, Tertel, Tobias, additional, Tigges, John, additional, De Wever, Olivier, additional, Yan, Xiaomei, additional, Nieuwland, Rienk, additional, Wauben, Marca H.M., additional, Nolan, John P., additional, and Jones, Jennifer C., additional

Published
2020
Full Text
View/download PDF

16. A Systematic Approach to Improve Scatter Sensitivity of a Flow Cytometer for Detection of Extracellular Vesicles.

Author

de Rond L, van der Pol E, Bloemen PR, Van Den Broeck T, Monheim L, Nieuwland R, van Leeuwen TG, and Coumans FAW

Subjects
Flow Cytometry, Light, Polystyrenes, Extracellular Vesicles
Abstract

Extracellular vesicles (EVs) are commonly studied by flow cytometry. Due to their small size and low refractive index, the scatter intensity of most EVs is below the detection limit of common flow cytometers. Here, we aim to improve forward scatter (FSC) and side scatter (SSC) sensitivity of a common flow cytometer to detect single 100 nm EVs. The effects of the optical and fluidics configuration on scatter sensitivity of a FACSCanto (Becton Dickinson) were evaluated by the separation index (SI) and robust coefficient of variation (rCV) of polystyrene beads (BioCytex). Improvement is defined as increased SI and/or reduced rCV. Changing the obscuration bar improved the rCV 1.9-fold for FSC. A 10-fold increase in laser power improved the SI 19-fold for FSC and 4.4-fold for SSC, whereas the rCV worsened 0.8-fold and improved 1.5-fold, respectively. Confocalization worsened the SI 1.2-fold for FSC, and improved the SI 5.1-fold for SSC, while the rCV improved 1.1-fold and worsened 1.5-fold, respectively. Replacing the FSC photodiode with a photomultiplier tube improved the SI 66-fold and rCV 4.2-fold. A 2-fold reduction in sample stream width improved both SI and rCV for FSC by 1.8-fold, and for SSC by 1.3- and 2.2-fold, respectively. Decreasing the sample flow velocity worsened rCVs. Decreasing the flow channel dimensions and the pore size of the sheath filter did not substantially change the SI or rCV. Using the optimal optical configuration and fluidics settings, the SI improved 3.8∙10 4 -fold on FSC and 30-fold on SSC, resulting in estimated detection limits for EVs (assuming a refractive index of 1.40) of 246 and 91 nm on FSC and SSC, respectively. Although a 50-fold improvement on FSC is still necessary, these adaptions have produced an operator-friendly, high-throughput flow cytometer with a high sensitivity on both SSC and FSC. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry., (© 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.)

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results