Search

Aryel H Ferreira,1– 3,* Fábio LN Marques,4 Caroline C Real,5 Velaphi C Thipe,6,* Lucas F Freitas,1,2 Caroline SA Lima,1,2 Larissa Estessi de Souza,4 Mara S Junqueira,7 Daniele de Paula Faria,4 Gustavo HC Varca,1 Ademar B Lugão,1 Kattesh V Katti6,8,* 1Nuclear and Energy Research Institute, IPEN-CNEN/SP, Sao Paulo, 05508-000, Brazil; 2MackGraphe - Mackenzie Institute for Research in Graphene and Nanotechnologies, Mackenzie Presbyterian University, Sao Paulo, 01302-907, Brazil; 3Mackenzie Evangelical College of Paraná - Mackenzie Presbyterian University, Curitiba-PR, 80730-000, Brazil; 4Laboratory of Nuclear Medicine (LIM-43), Departamento de Radiologia e Oncologia, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, 05403-911, Brazil; 5Aarhus University, Department of Nuclear Medicine & PET-Centre, Aarhus, Denmark; 6Department of Radiology, Institute of Green Nanotechnology, University of Missouri, Columbia, MO, 65212, USA; 7Center for Translational Research in Oncology, Cancer Institute of the State of Sao Paulo, Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil; 8Department of Biotechnology & Food Technology, University of Johannesburg, Johannesburg, South Africa*These authors contributed equally to this workCorrespondence: Kattesh V Katti, Department of Radiology, Institute of Green Nanotechnology, University of Missouri, Columbia, MO, 65212, USA, Email kattik@health.missouri.edu Aryel H Ferreira, MackGraphe-Mackenzie Institute for Research in Graphene and Nanotechnologies, Mackenzie Presbyterian University, Consolação Street 930, São Paulo, 01302-907, Brazil, Email aryel.ferreira@mackenzie.brBackground: Recent advancements in nanomedicine and nanotechnology have expanded the scope of multifunctional nanostructures, offering innovative solutions for targeted drug delivery and diagnostic agents in oncology and nuclear medicine. Nanoparticles, particularly those derived from natural sources, hold immense potential in overcoming biological barriers to enhance therapeutic efficacy and diagnostic accuracy. Papain, a natural plant protease derived from Carica papaya, emerges as a promising candidate for green nanotechnology-based applications due to its diverse medicinal properties, including anticancer properties.Purpose: This study presents a novel approach in nanomedicine and oncology, exploring the potential of green nanotechnology by developing and evaluating technetium-99m radiolabeled papain nanoparticles (99mTc-P-NPs) for imaging breast tumors. The study aimed to investigate the efficacy and specificity of these nanoparticles in breast cancer models through preclinical in vitro and in vivo assessments.Methods: Papain nanoparticles (P-NPs) were synthesized using a radiation-driven method and underwent thorough characterization, including size, surface morphology, surface charge, and cytotoxicity assessment. Subsequently, P-NPs were radiolabeled with technetium-99m (99mTc), and in vitro and in vivo studies were conducted to evaluate cellular uptake at tumor sites, along with biodistribution, SPECT/CT imaging, autoradiography, and immunohistochemistry assays, using breast cancer models.Results: The synthesized P-NPs exhibited a size mean diameter of 9.3 ± 1.9 nm and a spherical shape. The in vitro cytotoxic activity of native papain and P-NPs showed low cytotoxicity in HUVEC, MDA-MB231, and 4T1 cells. The achieved radiochemical yield was 94.2 ± 3.1% that were sufficiently stable (≥ 90%) for 6 h. The tumor uptake achieved in the 4T1 model was 2.49 ± 0.32% IA/g at 2 h and 1.51 ± 0.20% IA/g at 6 h. In the spontaneous breast cancer model, 1.19 ± 0.20% IA/g at 2 h and 0.86 ± 0.31% IA/g at 6 h. SPECT/CT imaging has shown substantial tumor uptake of the new nanoradiopharmaceutical and clear tumor visualization. 99mTc-P-NPs exhibited a high affinity to tumoral cells confirmed by ex vivo autoradiography and immunohistochemistry assays.Conclusion: The findings underscore the potential of green nanotechnology-driven papain nanoparticles as promising agents for molecular imaging of breast and other tumors through SPECT/CT imaging. The results represent a substantial step forward in the application of papain nanoparticles as carriers of diagnostic and therapeutic radionuclides to deliver diagnostic/therapeutic payloads site-specifically to tumor sites for the development of a new generation of nanoradiopharmaceuticals. Keywords: papain nanoparticle, protein nanoparticle, enzymatic nanoparticle, imaging probe, nanoradiopharmaceuticals

Gaetana Pezzino,1 Alessia Calabrò,1 Fabiana Drommi,1 Stefania Campana,1 Riccardo Cavaliere,1,2 Irene Bonaccorsi,1 Paolo Carrega,1 Vincenzo Zammuto,3– 5 Maria Giovanna Rizzo,3 Concetta Gugliandolo,3,4 Guido Ferlazzo,6,7 Claudia De Pasquale1 1Laboratory of Immunology and Biotherapy, Department Human Pathology ”G. Barresi”, University of Messina, Messina, Italy; 2Division of Clinical Pathology, University Hospital Policlinico G. Martino, Messina, Italy; 3Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy; 4Research Centre for Extreme Environments and Extremophiles, Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy; 5ATHENA Green Solutions S.r.l., Messina, Italy; 6Department of Experimental Medicine (DIMES), University of Genoa, Genova, Italy; 7Unit of Experimental Pathology and Immunology, IRCCS Ospedale Policlinico San Martino, Genova, ItalyCorrespondence: Guido Ferlazzo, Unit of Experimental Pathology and Immunology, IRCCS Ospedale Policlinico San Martino, Genoa, Italy, Email guido.ferlazzo@unige.itBackground: Respiratory viral infections are a leading cause of severe diseases and mortality; therefore, novel treatments effective for their prevention are highly requested. Here, we identified a broad-spectrum antiviral activity of a natural exopolysaccharide, EPS T14, purified from a marine thermotolerant strain of Bacillus licheniformis strain T14.Methods: The effects on human normal nasal epithelial cells (HNEpCs) following treatment with EPS T14 was evaluated at different time points and with increasing concentration of compound. To assess the antiviral properties, viability of HNEpCs treated with EPS T14 was analysed following infection with different respiratory viruses.Results: Neither toxicity nor pro-inflammatory properties were observed in vitro on HNEpCs treated with EPS T14 up to high concentrations, thus ensuring its safety. Cell culture-based assays revealed that treatment of HNEpCs with EPS T14 (used at 400ug/mL) results in efficient prevention of cell infection by different respiratory viruses through physically hindering the entry of the viruses via cell surface receptors. Interestingly, in addition to this prophylactic antiviral activity, EPS T14 also shows a long-lasting efficacy by inhibiting viral spread in the cell culture. Finally, combination of EPS T14 with a hypertonic saline solution shows a synergistic antiviral activity.Conclusion: EPS T14 can exert both prophylactic and therapeutic antiviral activity by blocking viral attachment to cellular receptors and could therefore represent a promising antiviral agent for preventing infections by different respiratory viruses. Keywords: exopolysaccharides, infection prophylaxis, nasal epithelium, respiratory virus, viral infection, EPSs

The main difference between a dry cell battery and an alkaline one is the composition of the electrolyte. In zinc–carbon batteries, dry cell, the electrolyte is a paste formed by mixing ammonium chloride and zinc chloride, whereas in alkaline batteries, the electrolyte is a concentrated aqueous solution of potassium hydroxide containing a certain amount of zinc oxide, hence the name alkaline for this battery. Therefore, the improper disposal of these materials has numerous consequences for the environment, since the potentially toxic metals present in them can be leached, infiltrating, and contaminating the soil layers, the groundwater, as well as the fauna and flora of the regions nearby. Thus, the objective is to perform studies that aim to simulate and analyze the release of potentially toxic metals present in batteries found in normal environmental conditions, through leaching tests from regular batteries (Zn–C) on fertilized soil, simulating a landfill, in addition to tests on sandy soil in order to aid the identification of possible waste release.

In 2019, right before arriving in Salvador, Brazil, to develop missionary activities, an international Protestant organization declared that this city – the center of the black culture in the country – was “known for its people’s belief in spirits and demons.” The statement soon triggered indignation from activists and devotees of Candomblé, the Afro-Brazilian religion that is a key element of the city’s history and identity. This article analyzes the transformation of this case into a public problem, which demanded different forms of reparation and affirmation of religious differences. The text debates the extension of Candomblé's semiotic community in Salvador and how the circulation of its symbols in different social contexts furthers the dissolution of boundaries between “religion” and “politics.” I pursue such ideas by describing a form of civil protest that moves liturgical forms from the Candomblé temples to public spaces. Such civil protest technology engages music, dance, and a set of materialities, presenting an instructive performance of the constitution of “religion” and the “public” in a post-colonial landscape.

Opsins play a key role in the ability to sense light both in image-forming vision and in non-visual photoreception (NVP). These modalities, in most animal phyla, share the photoreceptor protein: an opsin-based protein binding a light-sensitive chromophore by a lysine (Lys) residue. So far, visual and non-visual opsins have been discovered throughout the Metazoa phyla, including the photoresponsive Hydra, an eyeless cnidarian considered the evolutionary sister species to bilaterians. To verify whether light influences and modulates opsin gene expression in Hydra, we utilized four expression sequence tags, similar to two classic opsins (SW rhodopsin and SW blue-sensitive opsin) and two non-visual opsins (melanopsin and peropsin), in investigating the expression patterns during both diurnal and circadian time, by means of a quantitative RT-PCR. The expression levels of all four genes fluctuated along the light hours of diurnal cycle with respect to the darkness one and, in constant dark condition of the circadian cycle, they increased. The monophasic behavior in the L12:D12 cycle turned into a triphasic expression profile during the continuous darkness condition. Consequently, while the diurnal opsin-like expression revealed a close dependence on light hours, the highest transcript levels were found in darkness, leading us to novel hypothesis that in Hydra, an “internal” biological rhythm autonomously supplies the opsins expression during the circadian time. In conclusion, in Hydra, both diurnal and circadian rhythms apparently regulate the expression of the so-called visual and non-visual opsins, as already demonstrated in higher invertebrate and vertebrate species. Our data confirm that Hydra is a suitable model for studying ancestral precursor of both visual and NVP, providing useful hints on the evolution of visual and photosensory systems.

The role of hepatic venous pressure gradient (HVPG) measurement in risk stratification before liver resection is an ongoing area of debate. This study examines the impact of preoperative HVPG levels on overall survival (OS)/time to recurrence (TTR) and postoperative complications after hepatic resection of hepatocellular carcinoma (HCC). Thirty-eight HCC patients undergoing HVPG measurement before liver resection at Cambridge University Hospitals NHS Foundation Trust between January 2014 and April 2022 were retrospectively analysed. Statistical analysis comprised univariable/multivariable Cox/logistic regression to identify risk factors of reduced OS/TTR or 90-day post-resection complications and Kaplan–Meier estimator, log-rank, chi-squared, Fisher's exact, and Mann–Whitney U test, or Student's t-test for survival/subgroup analysis. The median HPVG was 6 (range: 0–14) mmHg. The HVPG was an independent risk factor for poorer TTR in the overall cohort (cut-off: ≥7.5 mmHg (17.18/43.81 months; P = 0.009)). In the subgroup analysis of cirrhotic patients (N = 29 (76%)), HVPG was additionally an independent risk factor for lower OS (cut-off: ≥8.5 mmHg [44.39/76.84 months; P = 0.012]). The HVPG had no impact on OS/TTR in non-cirrhotic patients (N = 9 (24%)), nor was it associated with postoperative complications in any cohort. In conclusion, preoperative HVPG levels are useful predictors for TTR and OS in cirrhotic HCC patients undergoing hepatic resection.

Brazil is home to a great diversity of species of the genus Dyckia. However, many of these species are threatened due to habitat destruction and predatory exploitation. An alternative to conserving these plants is sexual propagation using plant regulators to stimulate germination. Gibberellic acid (GA3) is an effective regulator in this process, but its instability and ease of degradation pose challenges. Therefore, nanoencapsulation of GA3 could be used to protect the molecule and allow controlled release. In this study, the effects of different doses of GA3 were evaluated on the germination of four species: D. cabrerae, D. dusenii, D. pottiorum and D. walteriana. The first stage consisted of soaking the seeds in different concentrations of GA3, in which the species D. dusenii and D. walteriana showed significant responses to GA3, with an increase from 35% to more than 60% germination. However, the species D. cabrerae and D. pottiorum responded positively to GA3 only in vegetative growth parameters. In the second stage, the use of nanoparticles of alginate/chitosan (NP ALG/CS) and chitosan/tripolyphosphate (NP CS/TPP) containing GA3 was compared with free GA3 and with NPs without GA3. It was verified that the use of nanoencapsulated GA3 resulted in a more efficient germination response in D. walteriana seeds, using smaller doses of the regulator (between 0.75 mg · L−1 and 1.0 mg · L−1), mainly with the ALG/ CS NPs. Therefore, the use of GA3 is recommended for D. dusenii and D. walteriana, and for the latter, nanoparticles containing ALG/CS-GA3 allow a reduction in the required dose.

Laura Manuela Pulgarin,1 Alessandro De Pellegrin,2 Giuseppe Stinco,3 Enzo Errichetti3 1Department of Dermatology, Universidad del Valle, Cali, Colombia; 2Department of Pathology, Udine “S. Maria della Misericordia” University Hospital, Udine, Italy; 3Institute of Dermatology, Department of Medicine, University of Udine, Udine, ItalyCorrespondence: Enzo Errichetti, Institute of Dermatology, Department of Medical Area, University of Udine, Piazzale Santa Maria della Misericordia, 15, Udine, 33100, Italy, Tel +39 0432559822, Email enzoerri@yahoo.it

Aldo Pezzuto,1 Alberto Ricci,1 Michela D’Ascanio,1 Alba Moretta,1 Giuseppe Tonini,2 Noemi Calabrò,1 Valeria Minoia,1 Alessia Pacini,1 Giuliana De Paolis,1 Eleonora Chichi,1 Elisabetta Carico,3 Antonella Tammaro4 1Department of Cardiovascular and Respiratory Sciences, S Andrea Hospital, Sapienza University, Rome, Italy; 2Oncology Department, Campus Bio-Medico University, Rome, Italy; 3Clinical and Molecular Medicine Department, S Andrea Hospital, Sapienza University, Rome, Italy; 4Department of Neuroscience- NESMOS, S.Andrea Hospital, Sapienza University, Rome, ItalyCorrespondence: Aldo Pezzuto, Email aldo.pezzuto@uniroma1.itBackground: Cigarette smoke exposure is the main preventable cause of chronic obstructive pulmonary disease (COPD). Airflow limitation is closely associated with smoking exposure. Smoking could also interfere with lipid metabolism.Aim: To determine the respiratory functional and metabolic changes after smoking cessation in smokers in the short term.Methods: All patients were current smokers. They were assessed by spirometry and questionnaires such as COPD assessment test(CAT), modified Medical Research Council (mMRC) test for dyspnea, Fagestrom’s test for nicotine dependence. Exhaled CO was detected in order to evaluate smoking exposure and smoking cessation (normal value< 7 ppm). A blood sampling was eventually taken for vitamin D and cholesterol assay. All patients underwent therapy with counselling and varenicline as first-line treatment according to its schedule. Detection time: at baseline and one month after smoking cessation.Results: All patients quit smoking during treatment. The mean age was 62 with a prevalence of males. The analysis revealed the following mean values at baseline: CAT mean score was 15, pack-years 35.5, Fagestrom’s Test mean score 5.0. The West’s value was 8.5, whereas Body mass index (BMI) was 25.5.Cigarette daily consumption mean value was 22.5. The comparison before and at follow up one month after smoking cessation about functional and metabolic parameters, show us the following results: FEV 1 was increased by 200 mL (p< 0.02), FEF 25/75 was improved as well as mMRC test. The eCO was dropped to as low as 8 ppM. Interestingly the vitamin D level was increased from 25 to 28 ng/mL without any support therapy. The cholesterol total level was reduced and CAT value and DLCO were also significantly improved.Conclusion: Quit smoking is useful to improve symptoms, respiratory function and metabolic parameters in the short term.Keywords: airflow limitation, smoking cessation, lipid metabolism

Lessons from Antarctica. For an ecology of the Solar System It seems plausible that the near future will see an ever greater expansion of human presence in several environments of the Solar System, such as the Moon and the planet Mars. This expansion will have multiple goals, of a scientific, exploitative-commercial, and perhaps military nature. This raises a series of ethical question, made even more pressing by the possibility that some of the extraterrestrial environments affected by human activities may host indigenous life forms. This article is meant to underline parallels and divergences of a future, hypothetical expansion of humanity in space with a process in many ways similar: human expansion in Antarctica. We intend to schematically describe the “Antarctic model”, i.e. the approach adopted so far by mankind as a whole towards the continent, highlighting its collective ethical, ecological and socio-political commitments and implications. Finally, on the basis of ideas taken from the literature on the philosophy of ecology, we will attempt to outline an “ecological mentality” that can be applied to the ethical problems relating to interaction with an extraterrestrial environment, be it entirely devoid of life or hosting a native biosphere.

La calprotectina (CP) es una proteína de unión a calcio y zinc que se suele determinar en muestras fecales, aunque su cuantificación en otros fluidos biológicos podría ser de interés. El objetivo del presente estudio es validar la determinación de CP en líquido pleural mediante quimioluminiscencia.

Introduction Physical restraint (PR) is prescribed in patients receiving invasive mechanical ventilation in the intensive care unit (ICU) to avoid unplanned removal of medical devices. However, it is associated with an increased risk of delirium. We hypothesise that a restrictive use of PR, as compared with a systematic use, could reduce the duration of delirium in ICU patients receiving invasive mechanical ventilation.Methods and analysis The Restrictive use of Restraints and Delirium Duration in ICU (R2D2-ICU) study is a national multicentric, parallel-group, randomised (1:1) open-label, controlled, superiority trial, which will be conducted in 10 ICUs. A total of 422 adult patients requiring invasive mechanical ventilation for an expected duration of at least 48 hours and eligible for prescription of PR will be randomly allocated within 6 hours from intubation to either the restrictive PR use group or the systematic PR use group, until day 14, ICU discharge or death, whichever comes first. In both groups, PR will consist of the use of wrist straps. The primary endpoint will be delirium or coma-free days, defined as the number of days spent alive in the ICU without coma or delirium within the first 14 days after randomisation. Delirium will be assessed using the Confusion Assessment Method-ICU twice daily. Key secondary endpoints will encompass agitation episodes, opioid, propofol, benzodiazepine and antipsychotic drug exposure during the 14-day intervention period, along with a core outcome set of measures evaluated 90 days postrandomisation.Ethics and dissemination The R2D2-ICU study has been approved by the Comité de Protection des Personnes (CPP) ILE DE FRANCE III—PARIS (CPP19.09.06.37521) on June 10th, 2019). Participant recruitment started on 25 January 2021. Results will be published in international peer-reviewed medical journals and presented at conferences.Trial registration number NCT04273360.

Background & Aims: Despite several recent international guidelines, no consensus exists on the bleeding risk nor haemostatic parameter thresholds that define the safety of invasive procedures in patients with cirrhosis. The aim of this study was to establish a position paper on the bleeding risk associated with invasive procedures in patients with cirrhosis among the experts involved in various guidelines. Methods: All experts involved in recent guidelines on the management of invasive procedures in patients with cirrhosis were invited to classify 80 procedures as ''high risk'' or ''low risk'' with respect to bleeding. Procedures were considered high risk when the estimated risk of major bleeding was 1.5% or more, or when even minor bleeding might lead to significant morbidity or death. The experts were also asked to choose safety thresholds for laboratory test values at which elective invasive procedures could be safely performed. The predetermined threshold considered as “consensus” was ≥75% agreement. Results: Fifty-two experts participated in the study. Out of 80 procedures, a consensus opinion was reached for 52 procedures (65%): 17 procedures were classified as “high risk”, primarily interventional endoscopic procedures, percutaneous organ biopsies, or procedures involving the central nervous system; and 35 as “low risk”, primarily “diagnostic” procedures. The lowest platelet counts at which performance of a low-risk procedure or a high-risk procedure/surgery were deemed acceptable were 30 × 109/L and 50 × 109/L, respectively. Experts did not believe that international normalised ratio should be considered before performing low-risk procedures; 71% also indicated that it should not be considered before performing high-risk procedures. Conclusions: This experience-based classification may be helpful to refine future study designs and to guide clinical decision making regarding invasive procedures in patients with cirrhosis. Impact and implications: Several risk classifications and management guidelines for invasive procedures in patients with cirrhosis have been proposed, but with conflicting recommendations. By providing a position paper, based on the opinion of a broad panel of experts, on the bleeding risk associated with 52 invasive procedures in patients with cirrhosis, this survey will help to provide a framework for future study design. The consensus on platelet count, international normalised ratio, fibrinogen and activated partial thromboplastin time identified in this survey will inform physicians regarding the laboratory test values considered acceptable by the experts prior to the performance of an elective invasive procedure in patients with cirrhosis.

Calprotectin (CP) is a calcium and zinc binding protein that is widely measured on faecal samples but its determination in other biological fluids might be of interest. The aim of this work was to validate the measurement of CP in pleural fluid by chemiluminescence.

Concetta De Pasquale,1 Noemi Barbagallo,2 Massimiliano Veroux,3 Maria Luisa Pistorio,2 Domenico Zerbo,2 Alessia Giaquinta,2 Burcin Ekser,4 Pierfrancesco Veroux2 1Department of Educational Sciences, University of Catania, Catania, Italy; 2Department of General Surgery and Medical-Surgical Specialties, University Hospital of Catania, Catania, Italy; 3Department of Surgical and Medical Sciences and Advanced Technologies, University Hospital of Catania, Catania, Italy; 4Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USACorrespondence: Maria Luisa Pistorio, Department of General Surgery and Medical-Surgical Specialties, University Hospital of Catania, Via Santa Sofia, 84, Catania, 95123, Italy, Tel/Fax +390953782629, Email marialuisa.pistorio@unict.itPurpose: To evaluate the association between emotional intelligence and fear of COVID-19 on self-reported adherence, based on a cross-sectional design.Patients and Methods: Transplants recipient of both sexes aged 23– 75 years old were evaluated at the Organ Transplant Unit, University Hospital of Catania, Italy. Data were analyzed using frequency, descriptives, Spearman and Pearson correlations, Chi-square goodness of fit test, and linear regression. Self-reported adherence was estimated with the Basel Assessment of Adherence with Immunosuppressive Medication Scale (BAASIS). Emotional intelligence and fear of COVID were, respectively, measured with the Emotional Intelligence Scale (EIS) and Multidimensional Assessment of COVID-19 Related Fears Scale (MAC-RF). This was a cross-sectional study of kidney transplant recipients. In reporting this study the authors followed the STROBE guidelines.Results: A correlation was found between EIS and MAC and between EIS and adherence but there was no correlation between MAC and adherence. A linear regression model was also conducted using a stepwise method, which indicated that EIS was a significant predictor of adherence (p < 0.05).Conclusion: This study was found that EIS is a predictor of adherence to treatment in transplant patients. Fear of COVID, while positively correlated to EIS, is not correlated to the adherence’s outcome, possibly due to the proper follow-up performed to the patients. Indeed, according to these results, we suggest to provide good follow-ups with recipients, with interviews also based on self-regulation and awareness.Keywords: adherence, kidney transplant, emotional intelligence, COVID-19

Giuseppe Forte,1 Giovanna Troisi,2 Francesca Favieri,1 Vilfredo De Pascalis,2 Viviana Langher,1 Maria Casagrande1 1Department of Dynamic and Clinical Psychology and Health Studies, Sapienza University of Rome, Roma, 00185, Italy; 2Department of Psychology, Sapienza University of Rome, Roma, 00185, ItalyCorrespondence: Giuseppe Forte; Maria Casagrande, Department of Dynamic and Clinical Psychology and Health Studies, Sapienza University of Rome, Roma, 00185, Italy, Email g.forte@uniroma1.it; maria.casagrande@uniroma1.itIntroduction: Pain is a complex experience that requires executive functions (EFs) to be processed. The autonomic outcome of the neural networks involved in the cognitive evaluation of pain is reflected by heart rate variability (HRV), an index of self-regulation abilities. Although some results suggest a relationship between HRV, EFs, and pain, studies focusing on this three-way relationship are still scarce.Objective: This study aims to investigate the relationship between pain, cognitive, and autonomic mechanisms, hypothesizing an association between resting HRV and both cognitive and motor inhibition as indices of executive functioning. This relationship was investigated after an experimental-induced pain.Methods: Seventy-six young adults were exposed to the Cold Pressure Arm Warp to induce experimental pain. HRV was collected, and cognitive tasks were administered to assess executive performance.Results: The results showed that (1) HRV indices significantly increased during pain stimulation, (2) cognitive inhibition was positively correlated with vagal indices and with pain parameters, (3) both inhibition tasks significantly predicted pain threshold while the performance on the Stroop Task predicted pain tolerance.Conclusion: Results suggest a three-way relationship. Further research would focus on the role of HRV and cognitive strategies in pain management in chronic pain conditions.Keywords: Stroop task, Go-NoGo, autonomic, pain, heart rate variability, cognition

Science misinformation represents a significant challenge for the scientific community. Hispanic communities are particularly vulnerable due to language barriers and the lack of accessible information in Spanish. We identified that a key step toward enhancing the accessibility of information for non-native English-speaking communities involves imparting science communication education and training to Hispanic youth. Our goal was to provide them with the skills to become science ambassadors who can effectively engage with their communities and bridge communication gaps. To address this, we developed the first science communication training program in Spanish for Hispanic high school and undergraduate students in Puerto Rico. The program called "+Ciencia" aims to provide training and education on science communication for Hispanic minorities through experiential and collaborative learning. In the short term, our multifaceted approach works to counter misinformation and promote science literacy within the broader community. Over the long term, our grassroots efforts with students will evolve into a generation of professionals equipped with strong engagement skills and comprehensive training in science communication with a specific focus on Hispanic audiences. Herein, we describe the components of this educational program and provide open access to educational materials and articles developed by three cohorts.

Introduction: Rhizoctonia solani, the causative agent of the sheath blight disease (ShB), invades rice to obtain nutrients, especially sugars; however, the molecular mechanism via which R. solani hijacks sugars from rice remains unclear. Objectives: In this study, rice-R. solani interaction model was used to explore whether pathogen effector proteins affect plant sugar absorption during infection. Methods: Yeast one-hybrid assay was used to identify Activator of SWEET2a (AOS2) from R. solani. Localization and invertase secretion assays showed that nuclear localization and secreted function of AOS2. Hexose transport assays verified the hexose transporter activity of SWEET2a and SWEET3a. Yeast two-hybrid assays, Bimolecular fluorescence complementation (BiFC) and transactivation assay were conducted to verify the AOS2-WRKY53-Grassy tiller 1 (GT1) transcriptional complex and its activation of SWEET2a and SWEET3a. Genetic analysis is used to detect the response of GT1, WRKY53, SWEET2a, and SWEET3a to ShB infestation. Also, the soluble sugar contents were measured in the mutants and overexpression plants before and after the inoculation of R. solani. Results: The present study found that R. solani protein AOS2 activates rice SWEET2a and localized in the nucleus of tobacco cells and secreted in yeast. AOS2 interacts with rice transcription factor WRKY53 and GT1 to form a complex that activates the hexose transporter gene SWEET2a and SWEET3a and negatively regulate rice resistance to ShB. Conclusion: These data collectively suggest that AOS2 secreted by R. solani interacts with rice WRKY53 and GT1 to form a transcriptional complex that activates SWEETs to efflux sugars to apoplast; R. solani acquires more sugars and subsequently accelerates host invasion.

The purpose of this article is to present the practice of price differentiation in multichannel sales conditions. The study reviews the literature on channel pricing policy. The authors set a goal to study the practices of retailers operating in online and offline channels in Poland in terms of price differentiation between channels. Using the example of the EMPIK and SMYK chains, price differences between online and offline channels were analysed for several 100 products in key categories for these retailers. The prices were obtained by scraping the data from the websites of both retailers in November 2022. Statistical analysis was designed to examine differences in pricing by product category, position on the “most frequently purchased” list and price range. This research confirmed previous results that online vs. offline price differentiation was not widely used by leading multichannel retailers in the most popular categories bought online: only two out of 12 retailers elicited for the study were found to perform it. It also confirmed previous findings that if price differentiation was applied, the items were cheaper online more often. However, the average depth of discount was considerably higher. Apart from these general findings, our research delivered detailed insights at the category level as the depth of discount and the share of products sold at a discount online considerably differed between categories with comparable and non-comparable offers. Additionally, this study provided a unique analysis of the multichannel price differentiation strategy in relation to popularity or the absolute price of the product. While in the case of the popularity of the product, the offer uniqueness seemed to play a role, the relation to the absolute price of the product showed a mixed picture and would need further investigation.

Christie RA Leite-Panissi,1 Bruna B De Paula,2 John K Neubert,3 Robert M Caudle2 1Department of Psychology, School of Philosophy, Science and Literature of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil; 2Department of Oral and Maxillofacial Surgery, University of Florida, Gainesville, FL, USA; 3Department of Orthodontics, University of Florida, Gainesville, FL, USACorrespondence: Robert M Caudle, Department of Oral and Maxillofacial Surgery, University of Florida, Gainesville, FL, USA. PO Box 100416. 1395 Center Drive, Gainesville, FL, 32610, USA, Tel +1 352-273-6767, Fax +1 352-392-7609, Email caudle@ufl.eduBackground: Temporomandibular joint (TMJ)-associated inflammation contributes to the pain reported by patients with temporomandibular disorders (TMD). It is common for patients diagnosed with TMD to report pain in the masticatory muscles and temporomandibular joints, headache, and jaw movement disturbances. Although TMD can have different origins, including trauma and malocclusion disorder, anxiety/depression substantially impacts the development and maintenance of TMD. In general, rodent studies on orofacial pain mechanisms involve the use of tests originally developed for other body regions, which were adapted to the orofacial area. To overcome limitations and expand knowledge in orofacial pain, our group validated and characterized an operant assessment paradigm in rats with both hot and cold stimuli as well mechanical stimuli. Nevertheless, persistent inflammation of the TMJ has not been evaluated with this operant orofacial pain assessment device (OPAD).Methods: We characterized the thermal orofacial sensitivity for cold, neutral, and hot stimuli during the development of TMD using the OPAD behavior test. In addition, we evaluated the role of transient receptor potential vanilloid 1 (TRPV1) expressing nociceptors in rats with persistent TMJ inflammation. The experiments were performed in male and female rats with TMJ inflammation induced by carrageenan (CARR). Additionally, resiniferatoxin (RTX) was administered into the TMJs prior CARR to lesion TRPV1-expressing neurons to evaluate the role of TRPV1-expressing neurons.Results: We evidenced an increase in the number of facial contacts and changes in the number of reward licks per stimulus on neutral (37°C) and cold (21°C) temperatures. However, at the hot temperature (42°C), the inflammation did not induce changes in the OPAD test. The prior administration of RTX in the TMJ prevented the allodynia and thermal hyperalgesia induced by CARR.Conclusion: We showed that TRPV-expressing neurons are involved in the sensitivity to carrageenan-induced pain in male and female rats evaluated in the OPAD.Keywords: temporomandibular disorder, acute pain, TRPV1, operant assay, orofacial pain

Climate change is being intensified by anthropogenic emission of greenhouse gasses, highlighting the value of forests for carbon dioxide storing carbon in their biomass. Seasonally dry tropical forests are a neglected, threatened, but potentially critical biome for helping mitigate climate change. In South America, knowing the amount and distribution of carbon in Caatinga seasonally dry vegetation is essential to understand its contribution to the global carbon cycle and subsequently design a strategic plan for its conservation. The present study aimed to model and map the spatial distribution of the potential forest biomass stock across 32 forest fragments of Caatinga, in the state of Bahia, Brazil, using regression kriging and Inverse Square of Distance techniques, building from point measurements of vegetation biomass made on-the-ground in ecological plots. First, a model for estimating biomass was fitted as a function of environmental variables to apply regression kriging, and then applied to the maps of the selected components. Elevation, temperature, and precipitation explained 46% of the biomass variations in the Caatinga. The model residuals showed strong spatial dependence and were mapped based on geostatistical criteria, selecting the spherical semivariogram model for interpolation by ordinary kriging. Biomass was also mapped by the Inverse Square of Distance approach. The quality of the regression model suggests that there is good potential for estimating biomass here from environmental variables. The regression kriging showed greater detail in the spatial distribution and revealed a spatial trend of increasing biomass from the north to south of the domain. Additional studies with greater sampling intensity and the use of other explanatory variables are suggested to improve the model, as well as to maximize the technique’s ability to capture the actual biomass behavior in this newly studied seasonally dry ecosystem.

Asif H Khan,1 Katherine Kosa,2 Lucia De Prado Gomez,3 Diane Whalley,4 Siddhesh Kamat,5 Marci Clark6 1Sanofi, Chilly-Mazarin, France; 2Patient-Centered Outcomes Assessment, RTI Health Solutions, Research Triangle Park, NC, USA; 3Sanofi, Reading, UK; 4Patient-Centered Outcomes Assessment, RTI Health Solutions, Manchester, UK; 5Regeneron Pharmaceuticals, Inc, Tarrytown, NY, USA; 6Patient-Centered Outcomes Assessment, RTI Health Solutions, Ann Arbor, MI, USACorrespondence: Asif H Khan, Sanofi, Chilly-Mazarin, France, Tel +33 1 60 49 50 76, Email Asif.Khan@sanofi.comPurpose: Sleep disturbance is common in patients with asthma and can lead to subsequent impacts on health-related quality of life (HRQOL). Fit-for-purpose patient-reported outcome measures (PROMs) assessing asthma-related sleep disturbance and next-day HRQOL impact (next-day impact) are needed to evaluate disease burden and treatment effects.Patients and Methods: Adults (18– 65 years) from three US clinics were recruited for semistructured interviews. Concept elicitation (CE) identified how asthma affects participants’ sleep and how asthma-related sleep disturbances impact their daily lives, which informed conceptual model development. Cognitive debriefing (CD) of the Asthma Sleep Disturbance Questionnaire (ASDQ), Sleep Diary, and Patient-Reported Outcomes Measurement Information System Sleep-Related Impairment Short Form 8a (PROMIS SRI SF8a) was completed to assess each measure’s content validity.Results: Twelve individuals participated in two interview rounds (6 individuals per round). Participants most frequently reported asthma-related nighttime awakening and decreased sleep quality and duration. Negative impacts of a poor night’s sleep due to asthma symptoms included feeling tired/fatigue/lack of energy and subsequent negative impacts on physical functioning, emotions and mood, mental functioning, work or volunteerism, and social functioning. Across both rounds of CD interviews, participants generally found the Sleep Diary and PROMIS SRI SF8a items relevant and easy to complete with no modifications. The ASDQ was modified for clarity and consistency.Conclusion: As described in the conceptual model, asthma affects multiple aspects of sleep that can cause next-day fatigue and other subsequent negative HRQOL impacts. This study demonstrates that the ASDQ, Sleep Diary, and PROMIS SRI SF8a items are comprehensive, relevant, and appropriate for patients with moderate-to-severe, uncontrolled asthma. Evaluation of psychometric properties for the ASDQ, Sleep Diary, and PROMIS SRI SF8a based on clinical trial data in patients with moderate-to-severe, uncontrolled asthma will further support their use.Keywords: asthma, sleep disturbance, patient-reported sleep measures

Drying operations in iron ore processing plants have a particularly high energy demand due to the massive solid flow rates employed in this industry. A 33 full-factorial design was applied to investigate the effects of air temperature, airflow velocity, and solids load on the drying time and the specific energy consumption (SEC) of the convective drying of iron ore fines in a fixed bed. The results demonstrated that each drying air condition was associated with an optimal solids load that minimized the SEC. A load of 73 g (bed height of about 0.8 cm) was identified and validated as the optimal condition in terms of energy consumption for the configuration with the highest air temperature (90°C) and airflow velocity (4.5 m/s). This condition resulted in a drying time of 29.0 s and a corresponding SEC of 12.8 MJ/kg to reduce the moisture from 0.11 kg water/kg dry solids to a target of 0.05 kg water/kg dry solids. Identifying the optimum values for the process variables should assist in designing and operating energy-efficient convective dryers for iron ore fines.

Luigino Dal Maso,1 Armando Santoro,2,3 Elisabetta Iannelli,4,5 Paolo De Paoli,6 Carla Minoia,7 Monica Pinto,8 Alexia Francesca Bertuzzi,3 Diego Serraino,1 Roberta De Angelis,9 Annalisa Trama,10 Riccardo Haupt,11 Gabriella Pravettoni,12,13 Maria Perrone,14 Francesco De Lorenzo,4,5 Paolo Tralongo15 On behalf of the Alliance Against Cancer (ACC) Survivorship Care and Nutritional Support Working Group1Cancer Epidemiology Unit, Centro di Riferimento Oncologico di Aviano (CRO) IRCCS, Aviano, Italy; 2Department of Biomedical Sciences, Humanitas University, Milan, Italy; 3IRCCS Humanitas Research Hospital, Humanitas Cancer Center, Milan, Italy; 4Italian Federation of Cancer Patients Organisations (FAVO), Rome, Italy; 5Italian Association of Cancer Patients (Aimac), Rome, Italy; 6Alleanza Contro il Cancro, Rome, Italy; 7SC Haematology, IRCCS Istituto Tumori “Giovanni Paolo II”, Bari, Italy; 8Rehabilitation Medicine Unit, Strategic Health Services Department, Istituto Nazionale Tumori-IRCCS Fondazione G. Pascale, Naples, Italy; 9Department of Oncology and Molecular Medicine, Italian National Institute of Health (ISS), Rome, Italy; 10Evaluative Epidemiology Unit, Department of Research, Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Milano, Italy; 11DOPO Clinic, Department of Pediatric Haematology/Oncology, IRCCS Istituto Giannina Gaslini, Genoa, Italy; 12Department of Oncology and Hemato-Oncology, University of Milan, Milan, Italy; 13Applied Research Division for Cognitive and Psychological Science, IEO European Institute of Oncology IRCCS, Milan, Italy; 14Psychology Unit, IRCCS Regina Elena Cancer Institute, Rome, Italy; 15Medical Oncology Unit, Umberto I Hospital, Department of Oncology, RAO, Siracusa, ItalyCorrespondence: Luigino Dal Maso, Epidemiologia Oncologica, Centro di Riferimento Oncologico di Aviano (CRO) IRCCS, Via Franco Gallini 2, Aviano (PN), 33081, Italy, Tel +39 0434 659354, Email epidemiology@cro.it Paolo Tralongo, Medical Oncology Unit, Umberto I Hospital, Department of Oncology, RAO, Via Giuseppe Testaferrata 1, Siracusa, 96100, Italy, Tel +39 0931 724 464, Email paolo.tralongo@asp.sr.itAbstract: A multidisciplinary panel of experts and cancer patients developed a position paper to highlight recent evidence on “cancer cure” (ie, the possibility of achieving the same life expectancy as the general population) and discuss the consequences of this concept on follow-up and rehabilitation strategies. The aim is to inform clinicians, patients, and health-care policy makers about strategies of survivorship care for cured cancer patients and consequences impacting patient lives, spurring public health authorities and research organizations to implement resources to the purpose. Two identifiable, measurable, and reproducible indicators of cancer cure are presented. Cure fraction (CF) is > 60% for breast and prostate cancer patients, > 50% for colorectal cancer patients, and > 70% for patients with melanoma, Hodgkin lymphoma, and cancers of corpus uteri, testis (> 90%), and thyroid. CF was > 65% for patients diagnosed at ages 15– 44 years and 30% for those aged 65– 74 years. Time-to-cure was consistently < 1 year for thyroid and testicular cancer patients and < 10 years for patients with colorectal and cervical cancers, melanoma, and Hodgkin lymphoma. The working group agrees that the evidence allows risk stratification of cancer patients and implementation of personalized care models for timely diagnosis, as well as treatment of possible cancer relapses or related long-term complications, and preventive measures aimed at maintaining health status of cured patients. These aspects should be integrated to produce an appropriate follow-up program and survivorship care plan(s), avoiding stigma and supporting return to work, to a reproductive life, and full rehabilitation. The “right to be forgotten” law, adopted to date only in a few European countries, may contribute to these efforts for cured patients.Keywords: cancer cure, survivorship, time-to-cure, personalized care models, right to be forgotten

Christopher Hillis,1 Kathryn E Flynn,2 Erinn Hoag Goldman,3 Tracy S Moreira-Lucas,4 Josie Visentini,4 Stephanie Dorman,4 Rachel Ballinger,5 Hilary F Byrnes,6 Andrea De Palma,7 Valentin Barbier,8 Lisa Machado,9 Ehab Atallah2 1Department of Oncology, McMaster University, Hamilton, Canada; 2Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA; 3US Medical Affairs, Pfizer Inc, New York, NY, USA; 4Medical Affairs, Pfizer Canada Inc., Kirkland, Quebec, Canada; 5Patient Centred Outcomes (PCO), ICON Clinical Research Inc., Reading, UK; 6Patient Centred Outcomes (PCO), ICON Clinical Research Inc., Blue Bell, PA, USA; 7Patient Centred Outcomes (PCO), ICON Clinical Research Inc., Milan, Italy; 8Patient Centred Outcomes (PCO), ICON Clinical Research Inc., Lyon, France; 9The Canadian CML Network, Toronto, Ontario, CanadaCorrespondence: Andrea De Palma, Tel +39 06 45 20 8037, Email Andrea.DePalma@iconplc.comPurpose: With treatment, chronic myeloid leukemia (CML) has a favorable prognosis, however, individuals with CML experience impairment to their quality of life (QoL). The aim of this study was to examine the perspectives and experiences of individuals with CML and to understand their challenges communicating with their CML physician.Patients and Methods: An online survey in adults with CML (n=100) in the US and Canada assessed QoL, patient-provider relationships, treatment satisfaction, and understanding of CML and treatment goals via the MD Anderson Symptom Inventory, the Cancer Therapy Satisfaction Questionnaire and de novo survey questions. Participants were recruited via an external patient recruiter and CML Patient Groups.Results: Many participants reported hardships due to CML and its treatment. The main impacts were on the ability to work (21%), engage in personal activities (e.g., hobbies, 28%), and to enjoy sexual relations (median=2.00, IQR=8.50). A substantial proportion (21– 39%) wished to discuss additional topics with their providers (e.g., management of CML and/or its impacts). While participants reported satisfaction with therapy overall (median=85.71, IQR=17.86), they indicated low to moderate treatment satisfaction with specific components, including concerns regarding side effects (median=43.75, IQR=43.75). Participants generally had a good understanding of CML (97%) and its treatment goals (92%).Conclusion: These findings advance our understanding of issues that need improvement to support QoL for individuals living with CML. Future work is needed to improve patient-provider relationships, address treatment-related side effects, and provide clinical information that is easier for patients to understand.Keywords: chronic myeloid leukemia, quality of life, patient-health care provider relationship, patient experience, survey, North America

The current study investigated compliance resistance in various interpersonal contexts. The 242 respondents who answered an online questionnaire were asked to recall a situation where they refused to comply with a request and to rate the degree of their compliance resistance. They also rated certain factors that were predicted to be related to noncompliance. Results indicated that the three dimensions of self-perceived credibility, intention to refuse compliance, and perceived control over refusal to comply had statistically significant correlations with compliance resistance. Two relational components—immediate interpersonal trust and relational closeness—also had statistically significant correlations with the strength of refusal to comply. Post hoc analysis demonstrated that four of the variables tested in the current study could serve as predictors of compliance resistance.

Organic agricultural waste is successfully used in the production of mushrooms as it promotes the reuse of resources and increases food production. For the formulation of the substrate for Pleurotus spp. production, the availability of nutrients must be rigorously analyzed to assess whether supplementation is necessary. The aim of this paper was to evaluate the agronomic behavior of Pleurotus ostreatus var. Florida when grown on different straws and to analyze nutrient dynamics at the beginning and end of cultivation. Straws of rice, wheat and brachiaria were used as substrates without any supplementation. The yields, biological efficiency, number and weights of mushrooms were evaluated in two crop cycles. Rice straw provided a higher yield, better biological efficiency and a greater number of mushrooms in both cultures. Wheat straw showed intermediate results for the same variables. None of the substrates showed a significant difference for mushroom weight. In the first crop, only Mg and the number of mushrooms exhibited a positive correlation; in the second crop, only K and the yield of the 1st flush showed a positive correlation. With this study, it was possible to demonstrate that agricultural residues with higher levels of K increase P. ostreatus yield, and that Mg management affects the number of mushrooms for harvesting. The results will be a useful guide for efficient and sustainable mineral supplementation of the substrate.

Once the pandemic concluded, the Foreign Languages Department of a Chilean state university hired a Canadian company to implement a pre-designed language learning system (PLLS). This platform was to be used by all teachers and students, as it contained various activities to develop all four language skills, including pronunciation practice through AI-based voice recognition. This study explores the attitudes of 17 university teachers towards using these pre-elaborated resources, activities, and assessments in their communicative English and German courses. A mixed-method approach was used, involving a survey based on the Technology Adoption Model (TAM) and individual interviews. Descriptive statistics were obtained from the survey responses, and qualitative data were analysed using content analysis techniques. The results indicate that teachers' attitudes towards the PLLS were generally neutral to negative. Instructors expressed their concerns about the system's pre-designed content and perceived functionality. Perceived ease of use and usefulness were rated low, reporting difficulties in navigation and alignment with their teaching styles. Perceived enjoyment received the lowest rating, mentioning issues such as disconnected content and lack of progressive structure. Qualitative data revealed technical problems, increased workload, and concerns about the system's impact on student motivation and learning outcomes. While some positive aspects were noted, the overall attitude towards the PLLS was predominantly negative, highlighting the need for better alignment with pedagogical goals and improved implementation strategies.

Background Malnutrition is a frequent problem in oncology and is associated with reduced response to cancer treatments, increased drug-related toxicity, higher rates of clinical complications, reduced quality of life (QoL) and worse prognosis. Guidelines on clinical nutrition in oncology emphasise the usefulness of early assessment of nutritional status for a prompt identification of malnutrition and the implementation of effective interventions, but no real-world clinical data are available on the adequate management of nutritional support in patients with cancer in Italy.Methods and analysis This is an observational, longitudinal, multicentre registry of patients with a new diagnosis of cancer or metastatic disease, candidates for active treatment. They will be identified in at least 15 Italian oncological centres, members of the Alliance Against Cancer Working Group ‘Survivorship Care and Nutritional Support’. At least 1500 patients with cancer are expected to be enrolled each year. Detailed clinical and nutritional data will be collected by oncologists and clinical nutritionists during the visits foreseen in the clinical practice, through an ad hoc developed digital platform (e-Nutracare). The effects of malnutrition and nutritional support—at diagnosis and during follow-up—on overall survival and progression-free survival, as well as on patients’ symptoms and QoL, will be investigated.Ethics and dissemination The study protocol was approved by the Ethics Committee of the Fondazione IRCCS Policlinico San Matteo, Pavia, Italy and from the Ethics Committees of all other participating centres. An informed consent will be obtained from each patient enrolled in the study. Study findings will be disseminated through peer-reviewed journals, conferences and patients with cancer or professional associations. The registry will allow a better monitoring of the nutritional status of patients with cancer, promoting adequate and sustainable nutritional support, with the ultimate goal of improving the care and prognosis of these patients.

The Q&U Bolometric Interferometer for Cosmology (QL’BIC) is the first bolometric interferometer designed to measure the primordial B-mode polarization of the Cosmic Microwave Background (CMB). Bolometric interferometry is a novel technique that combines the sensitivity of bolometric detectors with the control of systematic effects that is typical of interferometry, both key features in the quest for the faint signal of the primordial B-modes. A unique feature is the so-called “spectral imaging”, i.e., the ability to recover the sky signal in several sub-bands within the physical band during data analysis. This feature provides an in-band spectral resolution of ∆v/v ~ 0.04 that is unattainable by a traditional imager. This is a key tool for controlling the Galactic foregrounds contamination. In this paper, we describe the principles of bolometric interferometry, the current status of the QU BIC experiment and future prospects.

Galaxy clusters are composed of dark matter, gas and stars. Their dark matter component, which amounts to around 80% of the total mass, cannot be directly observed but traced by the distribution of diffused gas and galaxy members. In this work, we aim to infer the cluster’s projected total mass distribution from mock observational data, i.e. stars, Sunyaev-Zeldovich, and X-ray, by training deep learning models. To this end, we have created a multiview images dataset from The Three Hundred simulation that is optimal for training Machine Learning models. We further study deep learning architectures based on the U-Net to account for single-input and multi-input models. We show that the predicted mass distribution agrees well with the true one.

Our study introduces a new machine learning algorithm for estimating 3D cumulative radial profiles of total and gas mass in galaxy clusters from thermal Sunyaev-Zel’dovich (SZ) effect maps. We generate mock images from 2522 simulated clusters, employing an autoencoder and random forest in our approach. Notably, our model makes no prior assumptions about hydrostatic equilibrium. Our results indicate that the model successfully reconstructs unbiased total and gas mass profiles, with a scatter of approximately 10%. We analyse clusters in various dynamical states and mass ranges, finding that our method’s accuracy and precision are consistent. We verify the capabilities of our model by comparing it with the hydrostatic equilibrium technique, showing that it accurately recovers total mass profiles without any bias.

In the quest for the faint primordial B-mode polarization of the Cosmic Microwave Background, three are the key requirements for any present or future experiment: an utmost sensitivity, excellent control over instrumental systematic effects and over Galactic foreground contamination. Bolometric Interferometry (BI) is a novel technique that matches them all by combining the sensitivity of bolometric detectors, the control of instrumental systematics from interferometry and a software-based, tunable, in-band spectral resolution due to its ability to perform band-splitting during data analysis (spectral imaging). In this paper, we investigate how the spectral imaging capability of BI can help in detecting residual contamination in case an over-simplified model of foreground emission is assumed in the analysis. To mimic this situation, we focus on the next generation of ground-based CMB experiment, CMB-S4, and compare its anticipated sensitivities, frequency and sky coverage with a hypothetical version of the same experiment based on BI, CMB-S4/BI, assuming that lineof-sight (LOS) frequency decorrelation is present in dust emission but is not accounted for during component separation. We show results from a Monte-Carlo analysis based on a parametric component separation method (FGBuster), highlighting how BI has the potential to diagnose the presence of foreground residuals in estimates of the tensor-to-scalar ratio r in the case of unaccounted Galactic dust LOS frequency decorrelation.

In the framework of the IMEGIN Large Program, we used the NIKA2 camera on the IRAM 30-m telescope to observe the edge-on galaxy NGC 891 at 1.15 mm and 2 mm and at a FWHM of 11.1” and 17.6”, respectively. Multiwavelength data enriched with the new NIKA2 observations fitted by the HerBIE SED code (coupled with the THEMIS dust model) were used to constrain the physical properties of the ISM. Emission originating from the diffuse dust disk is detected at all wavelengths from mid-IR to mm. while mid-lR observations reveal warm dust emission from compact H II regions. Indications of mm excess emission have also been found in the outer parts of the galactic disk. Furthermore, our SED fitting analysis constrained the mass fraction of the small (< 15 Å) dust grains. We found that small grains constitute 9.5% of the total dust mass in the galactic plane, but this fraction increases up to ~ 20% at large distances (|z| > 3 kpc) from the galactic plane.

Galaxy clusters and surrounding medium, can be studied using X-ray bremsstrahlung emission and Sunyaev Zel’dovich (SZ) effect. Both astrophysical probes, sample the same environment with different parameters dependance. The SZ effect is relatively more sensitive in low density environments and thus is useful to study the filamentary structures of the cosmic web. In addition, observations of the matter distribution require high angular resolution in order to be able to map the matter distribution within and around galaxy clusters. MISTRAL is a camera working at 90GHz which, once coupled to the Sardinia Radio Telescope (SRT), can reach 12″ angular resolution over 4′ field of view (f.o.v.). The forecasted sensitivity drives to a Noise Equivalent Flux Density of ≃ 10–15 mJy √s and the mapping speed is MS = 380′2 mJy−2 h−1. MISTRAL was recently installed at the focus of the SRT and soon will take its first photons.

OLIMPO is a proposed Antarctic balloon-borne Sunyaev-Zel’dovich effect (SZE) imager to study gas dynamics associated with structure formation along with the properties of the warm-hot intergalactic medium (WHIM) residing in the connective filaments. During a 25 day flight OLIMPO will image a total of 10 z∼0.05 galaxy clusters and 8 bridges at 145, 250, 365, and 460 GHz at an angular resolution of 1.0′–3.3′. The maps will be significantly deeper than those planned from CMB-S4 and CCAT-P, and will have excellent fidelity to the large angular scales of our low-z targets, which are difficult to probe from the ground. In combination with X-ray data from eROSITA and XRISM we will transform our current static view of galaxy clusters into a full dynamic picture by measuring the internal intra-cluster medium (ICM) velocity structure with the kinematic SZE, X-ray spectroscopy, and the power spectrum of ICM fluctuations. Radio observations from ASKAP and MeerKAT will be used to better understand the connection between ICM turbulence and shocks with the relativistic plasma. Beyond the cluster boundary, we will combine thermal SZE maps from OLIMPO with X-ray imaging from eROSITA to measure the thermodynamics of the WHIM residing in filaments, providing a better understanding of its properties and its contribution to the total baryon budget.

The abundance of galaxy clusters with mass and redshift is a wellknown cosmological probe. The cluster mass is a key parameter for studies that aim to constrain cosmological parameters using galaxy clusters, making it critical to understand and properly account for the errors in its estimates. Subsequently, it becomes important to correctly calibrate scaling relations between observables like the integrated Compton parameter and the mass of the cluster. The NIKA2 Sunyaev-Zeldovich Large program (LPSZ) enables one to map the intracluster medium profiles in the mm–wavelength band with great details (resolution of 11 & 17″ at 1.2 & 2 mm, respectively) and hence, to estimate the cluster hydrostatic mass more precisely than previous SZ observations. However, there are certain systematic effects which can only be accounted for with the use of simulations. For this purpose, we employ The Three Hundred simulations which have been modelled with a range of physics modules to simulate galaxy clusters. The so-called twin samples are constructed by picking synthetic clusters of galaxies with properties close to the observational targets of the LPSZ. In particular, we use the Compton parameter maps and projected total mass maps of these twin samples along 29 different lines of sight. We investigate the scatter that projection induces on the total masses. Eventually, we consider the statistical values along different lines of sight to construct a kind of 3D scaling law between the integrated Compton parameter, total mass, and overdensity of the galaxy clusters to determine the overdensity that is least impacted by the projection effect.

This article aims to contribute to the debate on the role of the Quota Law regarding the potential social mobility of students with high socioeconomic adversity, using administrative data from a federal university in Brazil. We used Confirmatory Factor Analysis technique to construct an adversity index, composed of variables that may negatively affect access to higher education, such as students' socioeconomic background, parental education, and ethnicity. We classified courses by expected earnings and assessed how background is associated with course choice. Then, we used regression models estimated by the Generalized Method of Moments (GMM) to identify the relative importance of the interaction between quota categories with the adversity index and the score on the admission exam (National High School Examination -- "Exame Nacional do Ensino Médio" -- ENEM) to predict course choice and consequent possibility of social mobility. The results showed that quotas can reduce the negative effects of socioeconomic adversity on social mobility. However, they are not completely sufficient to "break" the effects of the low quality of secondary education in relation to ENEM performance and, consequently, social mobility

Céline Wessa,1,2 Manuel Morrens,1,2 Livia J De Picker1,2 1Scientific Initiative for Neuropsychiatric and Psychopharmacological Studies (SINAPS), University Psychiatric Hospital Campus Duffel, Duffel, Belgium; 2Collaborative Antwerp Psychiatric Research Institute (CAPRI), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, BelgiumCorrespondence: Céline Wessa, Email celine.wessa@uantwerpen.be

Greening is a subsidy provided by the Common Agricultural Policy (CAP), related to mowing and designed to protect environment. National or regional paying agencies (PP) monitor and verify compliance of farmers’ declarations with CAP rules. In this work, an operational procedure is proposed aimed at supporting PPs in detecting, mapping and quantifying the number of times mowing occurred in a meadow field. In particular, 72,539 meadows fields within the Piemonte region (NW – Italy) were analysed with a time series of Sentinel-2 (S2) data. The procedure is based on the processing of filtered and regularized time series of NDVI maps. The Fast Fourier Transform (FFT) was applied at field level to decompose the local NDVI temporal profile. The frequency ([Formula: see text] corresponding to the maximum amplitude ([Formula: see text] was therefore considered. [Formula: see text] value was used to detect not-mowed meadows by thresholding based on the application of the non-parametric Kolmogorov-Smirnov test. Mowing counting were achieved with reference to [Formula: see text] and the correspondent map (called MCM) generated for the study area. MCM was, finally, tested against the validation set (285 fields). Results showed an Overall Accuracy (OA) > 87%, confirming the effectiveness of the proposed procedure in detecting, mapping and quantifying the number of times mowing occurred.

Dans le cadre de l’élaboration d’une nouvelle collection de manuels d’enseignement de français en Suisse romande (« moyens d’enseignement romands de français », ou « MER-Français ») couvrant l’ensemble de la scolarité obligatoire (élèves de 4 à 14-15 ans), une équipe de l’Institut de recherche et de documentation pédagogique (IRDP) a été mandatée pour soutenir et accompagner une partie du travail de rédaction de ces manuels.Ces nouveaux moyens d’enseignement contiennent une série de « parcours », chacun portant sur un genre textuel particulier dont on vise à développer la maitrise chez les élèves. La définition et la description des différents genres retenus permettent de définir des objectifs d’apprentissage concrets, précis, liés au PER (Plan d’études Romand, 2010). C’est ce travail de « modélisation didactique » qui a été confié à l’IRDP, dans le but de contribuer au travail de l’équipe de rédaction des parcours. L’élaboration de tels modèles permet également d’esquisser une progression, dans une conception spiralaire reprenant les mêmes genres à différentes étapes de l’apprentissage et approfondissant ou complexifiant certains éléments de la mise en œuvre du genre considéré. Dans cette contribution, nous discuterons les notions linguistiques et didactiques qui sont à la base de notre travail de modélisation didactique. Sur la base de quelques exemples concernant les différents genres textuels pour lesquels nous avons développé une modélisation dans le cadre du projet, nous analyserons ensuite plus particulièrement le concept de « genre textuel » et celui de « regroupements de genres » – tel que ce dernier a été développé en Suisse romande –, en mettant en avant l’intérêt de la notion de genre et en soulignant à la fois les apports et les limites de ces concepts ainsi que leurs implications didactiques. Ce faisant, nous esquisserons une réflexion examinant comment et jusqu’à quel point on peut dépasser l’immense diversité des genres afin de construire une progression (et une distribution/répartition) des apprentissages aussi pertinente que possible.

En Forez, aux confins ouest du domaine francoprovençal, l’étiolement linguistique semble s’être déroulé, depuis les années 1925, sans un accroc, dans le silence des politiques locales, qui font pourtant grand cas de l’ancien parler des ouvriers stéphanois, le gaga. Cependant, le sociolinguiste qui se penche sur la situation actuelle du Forez a tôt fait de remarquer la permanence de comportements et de représentations sociales et linguistiques qui ne semblent pas dénuées de liens avec le patois disparu. Une série d’enquêtes de terrain menées de 2020 à 2023 a révélé la manière dont les systèmes socioéconomiques de la plaine et des monts du Forez, radicalement différents, ont entraîné des déroulements très divers du processus d’étiolement linguistique. L’insécurité linguistique aiguë ressentie par les ouvriers agricoles de la plaine, aux conditions de vie rudes, a mené à un rejet du patois et à une rupture de la transmission de sa mémoire, entachée de honte; dans les Monts en revanche, où la commune paysanne a fait corps face au développement des bourgs, la disparition du patois est simplement celle de la génération née avant et pendant la Seconde guerre mondiale. L’imaginaire linguistique forézien est, quoi qu’il en soit, peuplé de figures; le patois n’a plus d’existence que dans et par les souvenirs du monde où il était parlé. On le donc laisse mourir de sa belle mort. Bien que l’idéologie unilinguiste suscite encore à ce propos quelque malaise, on se plaît finalement à se le rappeler comme un souvenir d’enfance, et on s’empresse de désigner des hérauts d’armes, chargés de dicter la représentation métalinguistique collective. La patrimonialisation du patois est pourtant loin d’être amorcée. Les Foréziens redoutent qu’elle soit synonyme de dépossession de leur langue maternelle, à l’image de ce qu’il advint pour le parler gaga, extrait de son foyer d’origine, et souvent utilisé aujourd’hui pour faire rire les nouveaux venus à Saint-Étienne. Ils préfèrent parler du patois comme d’un héritage, qu’ils n’ont pas choisi, qui a été, souvent, cause de souffrance, mais qui, surtout, reste attaché aux noms de leurs anciens, à leurs sabots, et au monde dans lequel ils vécurent.

We study heavy-flavor hadron production in high-energy pp collisions, assuming the formation of a small, deconfined and expanding fireball where charm quarks can undergo rescattering and hadronization. We adopt the same in-medium hadronization mechanism developed for heavy-ion collisions, which involves Local Color-Neutralization (LCN) through recombination of charm quarks with nearby opposite color charges from the background fireball. Diquark excitations in the hot medium favor the formation of charmed baryons. The recombination process, involving closely aligned partons from the same fluid cell, effectively transfers the collective flow of the system to the final charmed hadrons. This framework can qualitatively reproduce the observed experimental findings in heavy-flavor particle-yield ratios, pT -spectra and ellipticflow coefficients. Our results provide new, complementary support to the idea that the collective phenomena observed in small systems have the same origin as those observed in heavy-ion collisions.

The Belle II software was developed by a few hundred individual contributors over several years. Following the rising desire of making it publicly available, the collaboration established open source software policies and procedures. The political and technical challenges and their solutions at Belle II are discussed in this article. With the publication of the Belle II software, basf2, on GitHub and Zenodo in 2021 an important milestone towards open science was reached.

Multi-messenger astrophysics provides valuable insights into the properties of the physical Universe. These insights arise from the complementary information carried by photons, gravitational waves, neutrinos and cosmic rays about individual cosmic sources and source populations. When a gravitational wave (GW) candidate is identified by the Ligo, Virgo and Kagra (LVK) observatory network, an alert is sent to astronomers in order to search for electromagnetic or neutrino counterparts. The current LVK framework for alert generation consists of the Gravitational-Wave Candidate Event Database (GraceDB), which provides a centralized location for aggregating and retrieving information about candidate GW events, the SCiMMA Hopskotch server (a publishsubscribe messaging system) and GWCelery (a package for annotating and orchestrating alerts). The first two services are deployed in the Cloud (Amazon Web Services), while the latter runs on dedicated physical resources. In this work, we propose a deployment strategy for the alert generation framework as a whole, based on Kubernetes. We present a set of tools (in the form of Helm charts, Python packages and scripts) which conveniently allows running a parallel deployment of the complete infrastructure in a private Cloud for scientific computing (the Cloud at CNAF, INFN Tier-1 Computing Centre), which is currently used for integration tests. As an outcome of this work, we deliver to the community a specific configuration option for a sandboxed deployment on Minikube, which can be used to test the integration of other components (i.e. the lowlatency pipelines for the detection of the GW candidate) with the alert generation infrastructure in an isolated local environment.

High-resolution mapping of the hot gas in galaxy clusters is a key tool for cluster-based cosmological analyses. Taking advantage of the NIKA2 millimeter camera operated at the IRAM 30-m telescope, the NIKA2 SZ Large Program seeks to get a high-resolution follow-up of 38 galaxy clusters covering a wide mass range at intermediate to high redshift. The measured SZ fluxes will be essential to calibrate the SZ scaling relation and the galaxy clusters mean pressure profile, needed for the cosmological exploitation of SZ surveys. We present in this study a method to infer a mean pressure profile from cluster observations. We have designed a pipeline encompassing the map-making and the thermodynamical properties estimates from maps. We then combine all the individual fits, propagating the uncertainties on integrated quantities, such as R500 or P500, and the intrinsic scatter coming from the deviation to the standard self-similar model. We validate the proposed method on realistic LPSZ-like cluster simulations.

Cluster number count is a major cosmological probe for the next generation of cosmological large scale-structure surveys like the one expected from the Euclid satellite mission. Cosmological constraints will be mainly limited by the understanding of the selection function (SF), which characterize the probability of detecting a cluster of a given mass and redshift. The SF can be estimated by injecting realistic simulated clusters into the survey and re-applying the detection procedure. In this paper, we use the galaxy clusters from The Three Hundred project to study this effect with their member galaxies. We further study possible resolution effects by comparing low and high resolution simulations. Finally, we present the density profiles of the member galaxies and discuss their evolution with cluster mass and redshift.