102 results on '"De Matteis V"'
Search Results
2. Psychotraumatisme, fonctionnement limite et addiction à l’adolescence : réévaluation de la notion de « comorbidité » pour l’hypothèse d’un « complexe » symptomatique et une nouvelle explicitation des perspectives thérapeutiques
- Author
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Shadili, G., Loisel, Y., Essadek, A., Nicolas, I., Pannetier, T., De Matteis, V., and Corcos, M.
- Published
- 2018
- Full Text
- View/download PDF
3. De la mémoire du corps à l’impulsion créatrice. Question de rythme
- Author
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de Matteis, V. and Corcos, M.
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- 2017
- Full Text
- View/download PDF
4. Psychotraumatisme, fonctionnement limite et addiction à l’adolescence : réévaluaton de la notion de « comorbidité » pour l’hypothèse d’un complexe symptomatique et une nouvelle explication des perspectives thérapeutiques
- Author
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Shadili, G., Ded, G., Loisel, Y., Nicolas, I., De Matteis, V., Essadek, Aziz, Pannetier, T., Corcos, Maurice, Laboratoire de psychologie de l'interaction et des relations intersubjectives (INTERPSY), Université de Lorraine (UL), Troubles du comportement alimentaire de l'adolescent (UMR_S 669), and Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Sud - Paris 11 (UP11)
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03 medical and health sciences ,Psychiatry and Mental health ,0302 clinical medicine ,Pediatrics, Perinatology and Child Health ,Developmental and Educational Psychology ,[SHS.PSY]Humanities and Social Sciences/Psychology ,16. Peace & justice ,030226 pharmacology & pharmacy ,ComputingMilieux_MISCELLANEOUS ,030227 psychiatry - Abstract
Resume Introduction La semiologie post-traumatique presente des symptomes caracterises notamment par des reviviscences du traumatisme. Ce tableau clinique peut s’enrichir des effets de la consommation puis de la dependance aux drogues. Des liens sont etablis aussi avec le trouble de personnalite limite. Nous revenons ici sur le concept de comorbidite et les entraves que cela peut constituer dans la prise en charge du sujet. Ce concept de comorbidite ressemble par trop a un discours mathematique qui privilegie l’etude des proportions au detriment de celle des relations, et qui risque d’aboutir au developpement d’analyse de structures supposees invariables. Methode Un symptome comorbide se doit d’etre etudie en reference au moment ou il survient, a l’etat psychopathologique ou il s’integre, a sa fonction dans celui-ci, et aux proprietes qu’il acquiert du fait de la place qu’il y prend. Resultats A partir de la nous insistons sur l’importance de l’affirmation diagnostique, mais aussi sur la strategie therapeutique qui conduit a articuler ce qui releve respectivement de l’intrapsychique et de l’intersubjectif, dans le cadre d’une demarche evaluative qui fait appel a la creativite du clinicien. Discussion De fait, notre option est de considerer les conduites addictives comme une tentative de « guerison » d’un trauma, une tentative d’echapper au retour de la reviviscence, meme si cela ne represente pas toutes les experiences addictives. Plus largement, le fonctionnement limite se concoit comme manifestations symptomatiques post-traumatique ou le trauma est d’emprise generationnelle.
- Published
- 2019
5. Gelatinous peritoneal disease: report of 14 cases
- Author
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Le Neel, J.-C., Letessier, E., Dupas, B., and de Matteis, V.
- Published
- 1997
6. Nanomaterials and Smart Nanodevices for Modular Dry Constructions: The Project 'easy House'
- Author
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De Matteis, V, Cannavale, A, Coppola, A, Fiorito, F, De Matteis, V, Cannavale, A, Coppola, A, and Fiorito, F
- Abstract
This paper reports the preliminary experimental results of a research project aiming at applying innovative materials and devices to a new modular construction system, named Easy House. The goal of the project is to use specific enabling nanotechnologies to achieve a significant enhancement of thermal performances of the building envelope as well as visual comfort indoor. With this aim, we perform a synthetic chemical route to obtain stable and monodispersed amorphous silica nanocapsules containing phase change materials (PCMs). The first results deriving from in vitro toxicological analyses, here reported, showed that such structures are not harmful and they can be adopted as suitable capsules to host a PCM, typically used in finishing materials, acting as thermal buffers. Moreover, the paper reports the possible advantages deriving from the integration of chromogenic devices, able to ensure energy saving and optimizing the use of daylighting.
- Published
- 2017
7. L'economia del Marocco e l'Italia
- Author
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DE MATTEIS, V.
- Published
- 1955
8. Identificazione dellimpatto al suolo delle mareggiate lungo le coste della Calabria: aspetti metodologici
- Author
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Petrucci, De Matteis V., and Versace
- Published
- 2005
9. Ricostruzione spazio-temporale degli effetti degli eventi alluvionali calabresi di dicembre 2002 - gennaio 2003
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Petrucci O. and De Matteis V.
- Published
- 2003
10. Aspetti metodologici nella identificazione dell'impatto al suolo degli eventi alluvionali
- Author
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Petrucci O., De Matteis V., and Versace P.
- Published
- 2003
11. THE IMPACT OF DAMAGING HYDRO-GEOLOGICAL EVENTS: A METHODOLOGICAL APPROACH OF HISTORICAL DATA INTERPRETATION
- Author
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Petrucci O. and Versace P. e De Matteis V.
- Published
- 2003
12. Eventi alluvionali calabresi di dicembre 2002-gennaio 2003: schede dei sopralluoghi
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Petrucci O., De Matteis V., and Longo M.A.
- Published
- 2003
13. Caratterizzazione delle condizioni di dissesto dell'abitato di S. Lorenzo Bellizzi (CS)
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Petrucci O. and De Matteis V.
- Published
- 2003
14. Lewis Acid-Promoted Synthesis and Reactivity of -O-Benzylhydroxylamino Imides Derived from -Glyceraldehyde
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Cardillo G., Gentilucci L., and De Matteis V.
- Published
- 2002
15. « THE ECONOMIC DEVELOPMENT OF NIGERIA » International Bank of Reconstruction and Development
- Author
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De Matteis, V.
- Published
- 1955
16. Aloe vera silver nanoparticles addition in chitosan films: improvement of physicochemical properties for eco-friendly food packaging material
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Valeria De Matteis, Mariafrancesca Cascione, Daniele Costa, Simona Martano, Daniela Manno, Alessandro Cannavale, Stefano Mazzotta, Fabio Paladini, Maurizio Martino, Rosaria Rinaldi, De Matteis, V, Cascione, M, Costa, D, Martano, S, Manno, D, Cannavale, A, Mazzotta, S, Paladini, F, Martino, M, and Rinaldi, R
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Chitosan Films, Green AgNPs, Physicochemical properties, Food packaging ,Biomaterials ,Food packaging ,Chitosan Films ,Green AgNPs ,Physicochemical properties ,Metals and Alloys ,Ceramics and Composites ,Surfaces, Coatings and Films - Abstract
In recent times, the searches for alternative materials to plastic is a popular topic, due to the danger that synthetic materials cause to the environment and humans. Among the promising natural polymers, chitosan (CS) is certainly one of the most suitable since it it is edible, non-toxic and derives from crustacean waste.- However, it is necessary to improve its physical properties to be widely applied in food packaging, whose market is dominated by synthetic plastic. In this work we have synthesized silver nanoparticles (AgNPs) using hot-plate and microwaves-based techniques, using Aloe Vera leaves extracts. The synthetic process follows the principles of green chemistry, since no toxic substance is used to obtain nanomaterials. These NPs, having dimensions
- Published
- 2023
17. Purification of olive mill wastewater through noble metal nanoparticle synthesis: waste safe disposal and nanomaterial impact on healthy hepatic cell mitochondria
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Chiara Ingrosso, Valeria De Matteis, Rosaria Rinaldi, Loris Rizzello, De Matteis, V., Rizzello, L., Ingrosso, C., and Rinaldi, R.
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Antioxidants perturbation ,Pollution ,Silver ,Physico-chemical propertie ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Industrial Waste ,Metal Nanoparticles ,Nanoparticle ,Wastewater ,010501 environmental sciences ,engineering.material ,Waste Disposal, Fluid ,01 natural sciences ,Silver nanoparticle ,Nanomaterials ,Metal ,Olea ,GTPase dynamin-related protein 1 expression ,Humans ,Environmental Chemistry ,Olive Oil ,Reusability of waste ,0105 earth and related environmental sciences ,media_common ,Chemistry ,food and beverages ,Mitochondria assessment ,General Medicine ,Pulp and paper industry ,Mitochondria ,Polyphenol ,visual_art ,Hepatocytes ,engineering ,visual_art.visual_art_medium ,Green synthesi ,Noble metal - Abstract
The exponential increase of waste derived from different human activities points out the importance of their reuse in order to create materials with specific properties that can be used for different applications. In this work, it was showed how the typical Mediterranean organic liquid waste, namely olive mill wastewater (OMWW), obtained during olive oil production, can be turned into an efficient reactive agent for the production of noble metals gold (Au) and silver nanoparticles (Ag NPs) with very well-defined physico-chemical properties. More than that, it was demonstrated that this synthetic procedure also leads to a drastic decrease of the organic pollution load of the OMWW, making it safer for environmental disposal and plants irrigation. Then, using healthy hepatic cell line mitochondria, the biological effects induced by these green metal NPs surrounded by a polyphenols shell, with the same NPs synthetized through a standard chemical colloidal reduction process, were compared, finding out that the green NPs are much safer. Graphical abstract: [Figure not available: see fulltext.].
- Published
- 2021
18. Sustainable sound absorbers obtained from olive pruning wastes and chitosan binder
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Alessandro Cannavale, Ubaldo Ayr, Francesco Martellotta, Valeria De Matteis, Martellotta, F., Cannavale, A., De Matteis, V., and Ayr, U.
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Absorption (acoustics) ,Materials science ,Acoustics and Ultrasonics ,Waste recycling ,0211 other engineering and technologies ,02 engineering and technology ,01 natural sciences ,Chitosan ,chemistry.chemical_compound ,021105 building & construction ,0103 physical sciences ,Phenomenological model ,Process engineering ,010301 acoustics ,Sound (geography) ,geography ,geography.geographical_feature_category ,business.industry ,Rigid frame ,Acoustics ,Sound Absorption ,Acoustics, Sound Absorption, Sustainable materials, Sustainability, Waste recycling ,Sustainability ,chemistry ,Sustainable materials ,business ,Pruning - Abstract
A significant interest in sustainable sound absorbing materials is fostering many researches on the use of recycled and vegetable products, that normally would have been sent to wastes, as compounds to obtain new building materials. One key issue when preparing new composite materials is the choice of the binder, which, in many cases, may significantly reduce the sustainability of the product by introducing synthetic and plastic elements. In the present paper olive tree pruning wastes bounded with chitosan are investigated to obtain sound absorbing materials. Experimental results showed that absorption coefficients as high as 0.9 can be obtained above 1 kHz using a 50 mm sample, and that a very good agreement with the phenomenological model for sound absorption through a rigid frame is obtained, suggesting that the model can be conveniently used to further improve the sound absorption characteristics of the samples.
- Published
- 2018
19. Physico-Chemical Properties of Inorganic NPs Influence the Absorption Rate of Aquatic Mosses Reducing Cytotoxicity on Intestinal Epithelial Barrier Model
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Makarena Rojas, Valeria De Matteis, Rosaria Rinaldi, Mariafrancesca Cascione, Gian Pietro Di Sansebastiano, Stefano Mazzotta, De Matteis, V., Rojas, M., Cascione, M., Mazzotta, S., Di Sansebastiano, G. P., and Rinaldi, R.
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Aquatic Organisms ,Chemical Phenomena ,Cytotoxicity ,Mosse ,Pharmaceutical Science ,Nanoparticle ,Metal Nanoparticles ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Analytical Chemistry ,mosses ,chemistry.chemical_compound ,Colloid ,QD241-441 ,X-Ray Diffraction ,Drug Discovery ,Microemulsion ,Intestinal Mucosa ,health care economics and organizations ,Titanium ,Caco-2 Cell ,Cell Death ,Aquatic Organism ,Chemistry ,respiratory system ,021001 nanoscience & nanotechnology ,Silver nitrate ,Chemistry (miscellaneous) ,Inorganic NP ,visual_art ,visual_art.visual_art_medium ,Molecular Medicine ,cytotoxicity ,0210 nano-technology ,Human ,inorganic chemicals ,Cell Survival ,Physico-chemical propertie ,Static Electricity ,Oxide ,Absorption (skin) ,Bryophyta ,Models, Biological ,physico-chemical properties ,Article ,Absorption ,Metal ,Metal Nanoparticle ,Inorganic Chemical ,mental disorders ,Humans ,Physical and Theoretical Chemistry ,0105 earth and related environmental sciences ,Organic Chemistry ,technology, industry, and agriculture ,inorganic NPs ,Dynamic Light Scattering ,Absorption, Physicochemical ,Inorganic Chemicals ,Titanium dioxide ,Spectrophotometry, Ultraviolet ,Caco-2 Cells ,Germ Cells, Plant ,absorption ,Nuclear chemistry - Abstract
Noble metals nanoparticles (NPs) and metal oxide NPs are widely used in different fields of application and commercial products, exposing living organisms to their potential adverse effects. Recent evidences suggest their presence in the aquifers water and consequently in drinking water. In this work, we have carefully synthesized four types of NPs, namely, silver and gold NPs (Ag NPs and Au NPs) and silica and titanium dioxide NPs (SiO2 NPs and TiO2 NPs) having a similar size and negatively charged surfaces. The synthesis of Ag NPs and Au NPs was carried out by colloidal route using silver nitrate (AgNO3) and tetrachloroauric (III) acid (HAuCl4) while SiO2 NPs and TiO2 NPs were achieved by ternary microemulsion and sol-gel routes, respectively. Once the characterization of NPs was carried out in order to assess their physico-chemical properties, their impact on living cells was studied. We used the human colorectal adenocarcinoma cells (Caco-2), known as the best representative intestinal epithelial barrier model to understand the effects triggered by NPs through ingestion. Then, we moved to explore how water contamination caused by NPs can be lowered by the ability of three species of aquatic moss, namely, Leptodictyum riparium, Vesicularia ferriei, and Taxiphyllum barbieri, to absorb them. The experiments were conducted using two concentrations of NPs (100 μM and 500 Μm as metal content) and two time points (24 h and 48 h), showing a capture rate dependent on the moss species and NPs type. Then, the selected moss species, able to actively capture NPs, appear as a powerful tool capable to purify water from nanostructured materials, and then, to reduce the toxicity associated to the ingestion of contaminated drinking water.
- Published
- 2021
20. Improvement of pmma dental matrix performance by addition of titanium dioxide nanoparticles and clay nanotubes
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Maria Luisa De Giorgi, Paolo Pellegrino, Fabio Paladini, Giovanni Albanese, Massimo Corsalini, Valeria De Matteis, Rosaria Rinaldi, Mariafrancesca Cascione, Cascione, M., De Matteis, V., Pellegrino, P., Albanese, G., De Giorgi, M. L., Paladini, F., Corsalini, M., and Rinaldi, R.
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Candida albican ,HNT ,Materials science ,Poly(methyl methacrylate) ,Biocompatibility ,Oral prosthese ,General Chemical Engineering ,wettability ,macromolecular substances ,engineering.material ,TiO2NPs ,Mechanical analysi ,Methacrylate ,Halloysite ,Article ,Nanomaterials ,Candida albicans ,oral prostheses ,General Materials Science ,Composite material ,Porosity ,HNTs ,QD1-999 ,technology, industry, and agriculture ,Adhesion ,mechanical analysis ,poly(methyl methacrylate) ,Chemistry ,visual_art ,visual_art.visual_art_medium ,engineering ,Wettability ,TiO2NP ,Wetting - Abstract
Over the last decades, several materials have been proposed for the fabrication of dental and mandibular prosthetic implants. Today, the poly(methyl-methacrylate) (PMMA) resin is the most spread material, due to its ease of processing, low cost, aesthetic properties, low weight, biocompatibility, and biostability in the oral cavity. However, the porous surface (which favors the adhesion of microorganisms) and the weak mechanical properties (which lead to wear or fracture) are the major concerns. The inclusion of engineered nanomaterials in the acrylic matrix could improve the performances of PMMA. In this study, we added two different kind of nanomaterials, namely titanium dioxide nanoparticles (TiO2NPs) and halloysite clay nanotubes (HNTs) at two concentrations (1% and 3% w/w) in PMMA. Then, we assessed the effect of nanomaterials inclusion by the evaluation of specific physical parameters: Young’s modulus, roughness, and wettability. In addition, we investigated the potential beneficial effects regarding the Candida albicans (C. albicans) colonization reduction, the most common yeast responsible of several infections in oral cavity. Our experimental results showed an improvement of PMMA performance, following the addition of TiO2NPs and HNTs, in a dose dependent manner. In particular, the presence of TiO2NPs in the methacrylate matrix induced a greater increase in PMMA stiffness respect to HNTs addition. On the other hand, HNTs reduced the rate of C. albicans colonization more significantly than TiO2NPs. The results obtained are of great interest for the improvement of PMMA physico-chemical properties, in view of its possible application in clinical dentistry.
- Published
- 2021
21. Colorimetric paper-based device for hazardous compounds detection in air and water: A proof of concept
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Valeria De Matteis, Rosaria Rinaldi, Gabriele Fella, Laura Mazzotta, Mariafrancesca Cascione, De Matteis, V., Cascione, M., Fella, G., Mazzotta, L., and Rinaldi, R.
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water ,02 engineering and technology ,lcsh:Chemical technology ,01 natural sciences ,Biochemistry ,Article ,Analytical Chemistry ,Hazardous waste ,colorimetric detection ,Environmental monitoring ,lcsh:TP1-1185 ,Electrical and Electronic Engineering ,Process engineering ,Instrumentation ,Volume concentration ,environmental monitoring ,Pollutant ,business.industry ,Atmosphere ,010401 analytical chemistry ,Water ,Paper based ,Contamination ,021001 nanoscience & nanotechnology ,Atomic and Molecular Physics, and Optics ,PAD ,0104 chemical sciences ,Chemical species ,Proof of concept ,atmosphere ,Environmental science ,0210 nano-technology ,business ,Colorimetric detection - Abstract
In the last decades, the increase in global industrialization and the consequent technological progress have damaged the quality of the environment. As a consequence, the high levels of hazardous compounds such as metals and gases released in the atmosphere and water, have raised several concerns about the health of living organisms. Today, many analytical techniques are available with the aim to detect pollutant chemical species. However, a lot of them are not affordable due to the expensive instrumentations, time-consuming processes and high reagents volumes. Last but not least, their use is exclusive to trained operators. Contrarily, colorimetric sensing devices, including paper-based devices, are easy to use, providing results in a short time, without particular specializations to interpret the results. In addition, the colorimetric response is suitable for fast detection, especially in resource-limited environments or underdeveloped countries. Among different chemical species, transition and heavy metals such as iron Fe(II) and copper Cu(II) as well as volatile compounds, such as ammonia (NH3) and acetaldehyde (C2H4O) are widespread mainly in industrialized geographical areas. In this work, we developed a colorimetric paper-based analytical device (PAD) to detect different contaminants, including Fe2+ and Cu2+ ions in water, and NH3 and C2H4O in air at low concentrations. This study is a &ldquo, proof of concept&rdquo, of a new paper sensor in which the intensity of the colorimetric response is proportional to the concentration of a detected pollutant species. The sensor model could be further implemented in other technologies, such as drones, individual protection devices or wearable apparatus to monitor the exposure to toxic species in both indoor and outdoor environments.
- Published
- 2020
22. Titanium Dioxide in Chromogenic Devices: Synthesis, Toxicological Issues, and Fabrication Methods
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Ubaldo Ayr, Valeria De Matteis, Alessandro Cannavale, De Matteis, V., Cannavale, A., and Ayr, U.
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Materials science ,Fabrication ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,synthetic approaches ,lcsh:Technology ,01 natural sciences ,lcsh:Chemistry ,chemistry.chemical_compound ,Fabrication methods ,General Materials Science ,Thin film ,lcsh:QH301-705.5 ,Instrumentation ,Fluid Flow and Transfer Processes ,Toxicity ,lcsh:T ,Chromogenic ,Chromogenic device ,Process Chemistry and Technology ,General Engineering ,toxicity ,021001 nanoscience & nanotechnology ,lcsh:QC1-999 ,0104 chemical sciences ,Computer Science Applications ,lcsh:Biology (General) ,lcsh:QD1-999 ,chemistry ,Titanium dioxide ,chromogenic devices ,lcsh:TA1-2040 ,Electrochromism ,Synthetic approache ,lcsh:Engineering (General). Civil engineering (General) ,0210 nano-technology ,lcsh:Physics - Abstract
The use of titanium dioxide (TiO2) within two specific classes of devices, namely electrochromic and photoelectrochromic, is described hereafter, with respect to its inherent properties and chromogenic features within architectures that have appeared so far, in this field. The new research trends, involving the applications of TiO2 in chromogenic materials are reported, with particular attention paid to the techniques used for film deposition as well as the synthesis of nanoparticles. Furthermore, the main studies concerning its chemical-physical properties and approaches to its chemical syntheses and fabrication are reviewed, with special regard to “green” routes. In addition, the main aspects relating to toxicological profiles are exposed, with reference to nanoparticles and thin films.
- Published
- 2020
23. Tuning cell behavior with nanoparticle shape
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Adrian Joseph, Loris Rizzello, Senio Campos De Souza, Edoardo Scarpa, Giuseppe Battaglia, Claudia Contini, Alessandro Poma, Josep Martí, Cesare De Pace, Valeria De Matteis, Eva Liatsi-Douvitsa, Scarpa, E., de Pace, C., Joseph, A. S., de Souza, S. C., Poma, A., Liatsi-Douvitsa, E., Contini, C., de Matteis, V., Marti, J. S., Battaglia, G., and Rizzello, L.
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Polymers ,PH ,Cell ,Apoptosis ,02 engineering and technology ,POLYMERSOMES ,Neoplasms ,Medicine and Health Sciences ,Nanotechnology ,ENCAPSULATION ,Internalization ,Cytotoxicity ,Materials ,media_common ,Staining ,Caspase 7 ,0303 health sciences ,Multidisciplinary ,Cell Death ,Chemistry ,Caspase 3 ,Vesicle ,021001 nanoscience & nanotechnology ,Up-Regulation ,Multidisciplinary Sciences ,medicine.anatomical_structure ,Oncology ,Macromolecules ,Cell Processes ,Drug delivery ,Physical Sciences ,Engineering and Technology ,Science & Technology - Other Topics ,Medicine ,Cellular Structures and Organelles ,0210 nano-technology ,Research Article ,Cyclin-Dependent Kinase Inhibitor p21 ,DNA Replication ,Cell type ,Cell Physiology ,General Science & Technology ,media_common.quotation_subject ,Science ,Materials Science ,Research and Analysis Methods ,VESICLES ,03 medical and health sciences ,SURFACE-CHEMISTRY ,DELIVERY ,Cell Line, Tumor ,medicine ,Centrifugation, Density Gradient ,Humans ,Cytoplasmic Staining ,030304 developmental biology ,Nanomaterials ,Science & Technology ,Cancers and Neoplasms ,Biology and Life Sciences ,Cell Biology ,IN-VITRO ,DNA ,Polymer Chemistry ,Cell Metabolism ,SIZE ,Specimen Preparation and Treatment ,Doxorubicin ,Hela Cells ,Polymersome ,Cancer cell ,Biophysics ,INTERNALIZATION ,Nanoparticles ,Tumor Suppressor Protein p53 - Abstract
We investigated how the shape of polymeric vesicles, made by the exact same material, impacts the replication activity and metabolic state of both cancer and non-cancer cell types. First, we isolated discrete geometrical structures (spheres and tubes) from a heterogeneous sample using density-gradient centrifugation. Then, we characterized the cellular internalization and the kinetics of uptake of both types of polymersomes in different cell types (either cancer or non-cancer cells). We also investigated the cellular metabolic response as a function of the shape of the structures internalized and discovered that tubular vesicles induce a significant decrease in the replication activity of cancer cells compared to spherical vesicles. We related this effect to the significant up-regulation of the tumor suppressor genes p21 and p53 with a concomitant activation of caspase 3/7. Finally, we demonstrated that combining the intrinsic shape-dependent effects of tubes with the delivery of doxorubicin significantly increases the cytotoxicity of the system. Our results illustrate how the geometrical conformation of nanoparticles could impact cell behavior and how this could be tuned to create novel drug delivery systems tailored to specific biomedical application.
- Published
- 2020
24. Green plasmonic nanoparticles and bio-inspired stimuli-responsive vesicles in cancer therapy application
- Author
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Valeria De Matteis, Loris Rizzello, Mariafrancesca Cascione, Eva Liatsi-Douvitsa, Azzurra Apriceno, Rosaria Rinaldi, De Matteis, V., Rizzello, L., Cascione, M., Liatsi-Douvitsa, E., Apriceno, A., and Rinaldi, R.
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Cancer therapy ,General Chemical Engineering ,Nanoparticle ,Nanotechnology ,Review ,lcsh:Chemistry ,Noble metals NPs ,General Materials Science ,Bio-inspired NP ,chemistry.chemical_classification ,Plasmonic nanoparticles ,Biomolecule ,Vesicle ,green synthesis ,technology, industry, and agriculture ,nanomedicine ,Nanomedicine ,chemistry ,lcsh:QD1-999 ,bio-inspired NPs ,Drug delivery ,noble metals NPs ,cancer therapy ,Nanorobotics ,Green synthesi - Abstract
In the last years, there is a growing interest in the application of nanoscaled materials in cancer therapy because of their unique physico-chemical properties. However, the dark side of their usability is limited by their possible toxic behaviour and accumulation in living organisms. Starting from this assumption, the search for a green alternative to produce nanoparticles (NPs) or the discovery of green molecules, is a challenge in order to obtain safe materials. In particular, gold (Au NPs) and silver (Ag NPs) NPs are particularly suitable because of their unique physico-chemical properties, in particular plasmonic behaviour that makes them useful as active anticancer agents. These NPs can be obtained by green approaches, alternative to conventional chemical methods, owing to the use of phytochemicals, carbohydrates, and other biomolecules present in plants, fungi, and bacteria, reducing toxic effects. In addition, we analysed the use of green and stimuli-responsive polymeric bio-inspired nanovesicles, mainly used in drug delivery applications that have revolutionised the way of drugs supply. Finally, we reported the last examples on the use of metallic and Au NPs as self-propelling systems as new concept of nanorobot, which are able to respond and move towards specific physical or chemical stimuli in biological entities.
- Published
- 2020
25. Synergistic Effect Induced by Gold Nanoparticles with Polyphenols Shell during Thermal Therapy: Macrophage Inflammatory Response and Cancer Cell Death Assessment
- Author
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Mariafrancesca Cascione, Claudia Di Guglielmo, Valeria De Matteis, Rosaria Rinaldi, Daniela Manno, Loris Rizzello, De Matteis, V., Cascione, M., Rizzello, L., Manno, D. E., Di Guglielmo, C., and Rinaldi, R.
- Subjects
Polyphenol ,Cancer Research ,DNA damage ,Physico-chemical propertie ,Inflammation ,02 engineering and technology ,AuNPs ,medicine.disease_cause ,physico-chemical properties ,Article ,03 medical and health sciences ,In vivo ,medicine ,cancer ,Thermal treatment ,Inflammation response ,Viability assay ,inflammation response ,RC254-282 ,polyphenols ,Cancer ,030304 developmental biology ,0303 health sciences ,Chemistry ,green synthesis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,food and beverages ,Photothermal therapy ,021001 nanoscience & nanotechnology ,medicine.disease ,AuNP ,Oncology ,Cancer cell ,Cancer research ,Green synthesi ,medicine.symptom ,0210 nano-technology ,thermal treatment ,Oxidative stress - Abstract
Simple Summary Polyphenols are present in a broad variety of plants, and they are known to possess anti-inflammation and anticancer properties. We used extracts from Laurus nobilis to synthetize gold nanoparticles (Au NPs), on which polyphenols were absorbed to form a stable shell (Au NPs@polyphenols). Then, the macrophage inflammation response was assessed, with the aim of using the Au NPs@polyphenols as synergistic tools in the thermal treatment of cancer cell models. We showed that the thermal conductivity enhancement, induced by Au during thermal treatment, increased the anticancer effects of polyphenols. After demonstrating the effectiveness of this system in in vitro cancer cell models, the future challenge will be the in vivo application of Au NPs@polyphenols. Abstract Background: In recent decades, gold nanoparticle (Au NP)-based cancer therapy has been heavily debated. The physico-chemical properties of AuNPs can be exploited in photothermal therapy, making them a powerful tool for selectively killing cancer cells. However, the synthetic side products and capping agents often induce a strong activation of the inflammatory pathways of macrophages, thus limiting their further applications in vivo. Methods: Here, we described a green method to obtain stable polyphenol-capped AuNPs (Au NPs@polyphenols), as polyphenols are known for their anti-inflammatory and anticancer properties. These NPs were used in human macrophages to test key inflammation-related markers, such as NF-κB, TNF-α, and interleukins-6 and 8. The results were compared with similar NPs obtained by a traditional chemical route (without the polyphenol coating), proving the potential of Au NPs@polyphenols to strongly promote the shutdown of inflammation. This was useful in developing them for use as heat-synergized tools in the thermal treatment of two types of cancer cells, namely, breast cancer (MCF-7) and neuroblastoma (SH-SY5Y) cells. The cell viability, calcium release, oxidative stress, HSP-70 expression, mitochondrial, and DNA damage, as well as cytoskeleton alteration, were evaluated. Results: Our results clearly demonstrate that the combined strategy markedly exerts anticancer effects against the tested cancer cell, while neither of the single treatments (only heat or only NPs) induced significant changes. Conclusions: Au NP@polyphenols may be powerful agents in cancer treatment.
- Published
- 2021
26. Encapsulation of Thermo-Sensitive Lauric Acid in Silica Shell: A Green Derivate for Chemo-Thermal Therapy in Breast Cancer Cell
- Author
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Maria Luisa De Giorgi, Mariafrancesca Cascione, Valeria De Matteis, Rosaria Rinaldi, Stefano Leporatti, De Matteis, V., Cascione, M., De Giorgi, M. L., Leporatti, S., and Rinaldi, R.
- Subjects
Pharmaceutical Science ,Thermal therapy ,Apoptosis ,Analytical Chemistry ,chemistry.chemical_compound ,0302 clinical medicine ,Drug Discovery ,Caspase ,chemistry.chemical_classification ,0303 health sciences ,biology ,Calorimetry, Differential Scanning ,Chemistry ,Coconut oil ,Temperature ,food and beverages ,Lauric Acids ,Silicon Dioxide ,Chemistry (miscellaneous) ,030220 oncology & carcinogenesis ,MCF-7 Cells ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) ,Female ,food.ingredient ,Cell Survival ,Drug Compounding ,Breast Neoplasms ,Article ,nanoencapsulation ,lcsh:QD241-441 ,03 medical and health sciences ,food ,breast cancer ,Breast cancer cell line ,lcsh:Organic chemistry ,Extracellular ,Humans ,Physical and Theoretical Chemistry ,030304 developmental biology ,Cell Nucleus ,Organic Chemistry ,Fatty acid ,lauric acid ,Spectrometry, X-Ray Emission ,Lauric acid ,Actins ,Dynamic Light Scattering ,biology.protein ,hyperthermal therapy ,Nanoparticles ,Breast cancer cells ,Reactive Oxygen Species ,Nuclear chemistry - Abstract
Lauric acid is a green derivate that is abundant in some seeds such as coconut oil where it represents the most relevant fatty acid. Some studies have emphasized its anticancer effect due to apoptosis induction. In addition, the lauric acid is a Phase Change Material having a melting temperature of about 43.2 °, C: this property makes it a powerful tool in cancer treatment by hyperthermal stress, generally induced at 43 °, C. However, the direct use of lauric acid can have some controversial effects, and it can undergo degradation phenomena in the extracellular environment. For this reason, we have encapsulated lauric acid in a silica shell with a one-step and reproducible synthetic route in order to obtain a monodispersed SiO2@LA NPs with a good encapsulation efficiency. We have used these NPs to expose breast cancer cell lines (MCF-7) at different concentrations in combination with hyperthermal treatment. Uptake, viability, oxidative stress induction, caspases levels, and morphometric parameters were analyzed. These nanovectors showed double action in anticancer treatments thanks to the synergic effect of temperature and lauric acid activity.
- Published
- 2019
27. Engineered Gold Nanoshells Killing Tumor Cells: New Perspectives
- Author
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Valeria De Matteis, Rosaria Rinaldi, Chiara Cristina Toma, Mariafrancesca Cascione, De Matteis, V., Cascione, M., Toma, C. C., and Rinaldi, R.
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Materials science ,Nanotechnology ,Tumor cells ,02 engineering and technology ,010402 general chemistry ,Gold shell ,01 natural sciences ,In vivo ,Cell Line, Tumor ,Neoplasms ,Drug Discovery ,Bio-conjugation ,Humans ,Photothermal ablation ,Surface plasmon resonance ,Pharmacology ,Nanoshells ,NIR ,Hyperthermia, Induced ,Photothermal therapy ,Phototherapy ,Surface Plasmon Resonance ,021001 nanoscience & nanotechnology ,Nanoshell ,0104 chemical sciences ,Drug delivery ,Cancer cell ,Bio-distribution ,Gold ,0210 nano-technology - Abstract
The current strategies to treat different kinds of cancer are mainly based on chemotherapy, surgery and radiation therapy. Unfortunately, these approaches are not specific and rather invasive as well. In this scenario, metal nano-shells, in particular gold-based nanoshells, offer interesting perspectives in the effort to counteract tumor cells, due to their unique ability to tune Surface Plasmon Resonance in different light-absorbing ranges. In particular, the Visible and Near Infrared Regions of the electromagnetic spectrum are able to penetrate through tissues. In this way, the light absorbed by the gold nanoshell at a specific wavelength is converted into heat, inducing photothermal ablation in treated cancer cells. Furthermore, inert gold shells can be easily functionalized with different types of molecules in order to bind cellular targets in a selective manner. This review summarizes the current state-of-art of nanosystems embodying gold shells, regarding methods of synthesis, bio-conjugations, bio-distribution, imaging and photothermal effects (in vitro and in vivo), providing new insights for the development of multifunctional antitumor drugs.
- Published
- 2019
28. Tailoring Cell Morphomechanical Perturbations Through Metal Oxide Nanoparticles
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Loris Rizzello, Valeria De Matteis, Chiara Cristina Toma, Rosaria Rinaldi, Paolo Pellegrino, Mariafrancesca Cascione, De Matteis, V., Cascione, M., Toma, C. C., Pellegrino, P., Rizzello, L., and Rinaldi, R.
- Subjects
Materials science ,Cytoskeleton rearrangement ,Cell ,Biomechanic ,Nanoparticle ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,lcsh:TA401-492 ,medicine ,General Materials Science ,Cytoskeleton rearrangements ,Biomechanics ,Young’s modulus ,Cytoskeleton ,Actin ,A549 cell ,Nano Express ,Toxicity ,Cell migration ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,In vitro ,0104 chemical sciences ,medicine.anatomical_structure ,Young’s Modulus ,Biophysics ,Nanoparticles ,lcsh:Materials of engineering and construction. Mechanics of materials ,0210 nano-technology ,Intracellular - Abstract
The nowadays growing use of nanoparticles (NPs) in commercial products does not match a comprehensive understanding of their potential harmfulness. More in vitro investigations are required to address how the physicochemical properties of NPs guide their engulfment within cells and their intracellular trafficking, fate, and toxicity. These nano-bio interactions have not been extensively addressed yet, especially from a mechanical viewpoint. Cell mechanic is a critical indicator of cell health because it regulates processes like cell migration, tissue integrity, and differentiation via cytoskeleton rearrangements. Here, we investigated in vitro the elasticity perturbation of Caco-2 and A549 cell lines, in terms of Young’s modulus modification induced by SiO2NPS and TiO2NPS. TiO2NPs demonstrated stronger effects on cell elasticity compared to SiO2NPs, as they induced significant morphological and morphometric changes in actin network. TiO2NPS increased the elasticity in Caco-2 cells, while opposite effects have been observed on A549 cells. These results demonstrate the existence of a correlation between the alteration of cell elasticity and NPs toxicity that depends, in turn, on the NPs physicochemical properties and the specific cell tested.
- Published
- 2019
29. Silver Nanoparticles: Synthetic Routes, In Vitro Toxicity and Theranostic Applications for Cancer Disease
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Chiara Cristina Toma, Valeria De Matteis, Stefano Leporatti, Mariafrancesca Cascione, De Matteis, V., Cascione, M., Toma, C. C., and Leporatti, S.
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Programmed cell death ,silver nanoparticles ,theranostics ,DNA damage ,General Chemical Engineering ,Silver nanoparticle ,Review ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,lcsh:Chemistry ,General Materials Science ,Secretion ,Toxicity ,Chemistry ,toxicity ,021001 nanoscience & nanotechnology ,In vitro ,0104 chemical sciences ,Theranostic ,lcsh:QD1-999 ,Apoptosis ,Drug delivery ,Synthetic route ,Biophysics ,0210 nano-technology ,synthetic routes - Abstract
The large use of nanomaterials in many fields of application and commercial products highlights their potential toxicity on living organisms and the environment, despite their physico-chemical properties. Among these, silver nanoparticles (Ag NPs) are involved in biomedical applications such as antibacterial agents, drug delivery vectors and theranostics agents. In this review, we explain the common synthesis routes of Ag NPs using physical, chemical, and biological methods, following their toxicity mechanism in cells. In particular, we analyzed the physiological cellular pathway perturbations in terms of oxidative stress induction, mitochondrial membrane potential alteration, cell death, apoptosis, DNA damage and cytokines secretion after Ag NPs exposure. In addition, their potential anti-cancer activity and theranostic applications are discussed.
- Published
- 2018
30. Toxicity assessment in the nanoparticle era
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Valeria De Matteis, Rosaria Rinaldi, De Matteis, V., and Rinaldi, R.
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0301 basic medicine ,In vitro and in vivo studie ,Animal ,Cytotoxicity ,Physico-chemical propertie ,Engineered nanomaterials ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,021001 nanoscience & nanotechnology ,03 medical and health sciences ,030104 developmental biology ,Exposure level ,Biodistribution ,Nanotoxicology ,Toxicity ,Nanotoxicity ,Business ,Human safety ,Safety ,0210 nano-technology ,Organism ,Potential toxicity ,Human - Abstract
The wide use of engineered nanomaterials in many fields, ranging from biomedical, agriculture, environment, cosmetic, urged the scientific community to understand the processes behind their potential toxicity, in order to develop new strategies for human safety. As a matter of fact, there is a big discrepancy between the increased classes of nanoparticles and the consequent applications versus their toxicity assessment. Nanotoxicology is defined as the science that studies the effects of engineered nanodevices and nanostructures in living organisms. This chapter analyzes the physico-chemical properties of the most used nanoparticles, the way they enter the living organism and their cytoxicity mechanisms at cellular exposure level. Moreover, the current state of nanoparticles risk assessment is reported and analyzed.
- Published
- 2018
31. Exposure to Inorganic Nanoparticles: Routes of Entry, Immune Response, Biodistribution and In Vitro/In Vivo Toxicity Evaluation
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Valeria De Matteis and De Matteis, V.
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0301 basic medicine ,Biodistribution ,Health, Toxicology and Mutagenesis ,Nanoparticle ,Nanotechnology ,Review ,02 engineering and technology ,Toxicology ,lcsh:Chemical technology ,Silver nanoparticle ,immune response ,03 medical and health sciences ,Immune system ,In vivo ,In vitro/in vivo toxicity ,lcsh:TP1-1185 ,Immune response ,biodistribution ,Chemical Health and Safety ,Chemistry ,routes of entry ,in vitro/in vivo toxicity ,Inorganic nanoparticle ,021001 nanoscience & nanotechnology ,inorganic nanoparticles ,030104 developmental biology ,Routes of entry ,Colloidal gold ,Toxicity ,0210 nano-technology ,Inorganic nanoparticles - Abstract
The development of different kinds of nanoparticles, showing different physico-chemical properties, has fostered their large use in many fields, including medicine. As a consequence, inorganic nanoparticles (e.g., metals or semiconductors), have raised issues about their potential toxicity. The scientific community is investigating the toxicity mechanisms of these materials, in vitro and in vivo, in order to provide accurate references concerning their use. This review will give the readers a thorough exploration on the entry mechanisms of inorganic nanoparticles in the human body, such as titanium dioxide nanoparticles (TiO₂NPs), silicon dioxide nanoparticles (SiO₂NPs), zinc oxide nanoparticles (ZnONPs), silver nanoparticles (AgNPs), gold nanoparticles (AuNPs) and quantum dots (QDsNPs). In addition, biodistribution, the current trends and novelties of in vitro and in vivo toxicology studies will be discussed, with a particular focus on immune response.
- Published
- 2017
32. Nanomaterials and Smart Nanodevices for Modular Dry Constructions: The Project 'Easy House'
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Alessandro Cannavale, Francesco Fiorito, Valeria De Matteis, Armando Coppola, De Matteis, V., Cannavale, A., Coppola, A., and Fiorito, F.
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Engineering ,020209 energy ,Sustainable housing, innovative building envelopes, nanomaterials, dry construction ,Nanotechnology ,02 engineering and technology ,Nanomaterials ,0202 electrical engineering, electronic engineering, information engineering ,Chemical route ,innovative building envelope ,Process engineering ,nanomaterials ,Sustainable housing ,innovative building envelopes ,business.industry ,General Medicine ,sustainable housing ,Modular construction ,Modular design ,dry construction ,nanomaterial ,Amorphous silica ,business ,Host (network) ,Building envelope ,Daylighting - Abstract
This paper reports the preliminary experimental results of a research project aiming at applying innovative materials and devices to a new modular construction system, named Easy House. The goal of the project is to use specific enabling nanotechnologies to achieve a significant enhancement of thermal performances of the building envelope as well as visual comfort indoor. With this aim, we perform a synthetic chemical route to obtain stable and monodispersed amorphous silica nanocapsules containing phase change materials (PCMs). The first results deriving from in vitro toxicological analyses, here reported, showed that such structures are not harmful and they can be adopted as suitable capsules to host a PCM, typically used in finishing materials, acting as thermal buffers. Moreover, the paper reports the possible advantages deriving from the integration of chromogenic devices, able to ensure energy saving and optimizing the use of daylighting.
- Published
- 2017
33. Cultivar-Dependent Anticancer and Antibacterial Properties of Silver Nanoparticles Synthesized Using Leaves of Different Olea Europaea Trees
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Maria Luisa De Giorgi, Chiara Ingrosso, Loris Rizzello, Giovanni De Matteis, Valeria De Matteis, Rosaria Rinaldi, Eva Liatsi-Douvitsa, De Matteis, V., Rizzello, L., Ingrosso, Carmela, Liatsi-Douvitsa, E., De Giorgi, M. L., De Matteis, G., and Rinaldi, R.
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silver nanoparticles ,Cytotoxicity ,General Chemical Engineering ,Population ,Nanoparticle ,Leccino ,Silver nanoparticle ,Nanomaterials ,Carolea ,lcsh:Chemistry ,HeLa ,antibacterial activity ,olea europaea ,General Materials Science ,Cultivar ,Olea europaea ,education ,education.field_of_study ,biology ,Chemistry ,green synthesis ,genotoxicity ,biology.organism_classification ,carolea ,lcsh:QD1-999 ,Olea ,cytotoxicity ,Green synthesi ,Antibacterial activity ,Genotoxicity ,Silver nanoparticles ,leccino ,Bacteria ,Nuclear chemistry - Abstract
The green synthesis of nanoparticles (NPs) is currently under worldwide investigation as an eco-friendly alternative to traditional routes (NPs): the absence of toxic solvents and catalysts make it suitable in the design of promising nanomaterials for nanomedicine applications. In this work, we used the extracts collected from leaves of two cultivars (Leccino and Carolea) belonging to the species Olea Europaea, to synthesize silver NPs (AgNPs) in different pH conditions and low temperature. NPs underwent full morphological characterization with the aim to define a suitable protocol to obtain a monodispersed population of AgNPs. Afterwards, to validate the reproducibility of the mentioned synthetic procedure, we moved on to another Mediterranean plant, the Laurus Nobilis. Interestingly, the NPs obtained using the two olive cultivars produced NPs with different shape and size, strictly depending on the cultivar selected and pH. Furthermore, the potential ability to inhibit the growth of two woman cancer cells (breast adenocarcinoma cells, MCF-7 and human cervical epithelioid carcinoma, HeLa) were assessed for these AgNPs, as well as their capability to mitigate the bacteria concentration in samples of contaminated well water. Our results showed that toxicity was stronger when MCF-7 and Hela cells were exposed to AgNPs derived from Carolea obtained at pH 7 presenting irregular shape, on the other hand, greater antibacterial effect was revealed using AgNPs obtained at pH 8 (smaller and monodispersed) on well water, enriched with bacteria and coliforms.
- Published
- 2019
34. Negligible particle-specific toxicity mechanism of silver nanoparticles: The role of Ag+ion release in the cytosol
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Antonio Galeone, Elisa De Luca, Valeria De Matteis, Prakash Kshirsagar, Sachin Sayaji Kote, Giuseppe Bardi, Pier Paolo Pompa, Maria Ada Malvindi, Stefania Sabella, Virgilio Brunetti, De Matteis, V., Malvindi, M. A., Galeone, A., Brunetti, V., De Luca, E., Kote, S., Kshirsagar, P., Sabella, S., Bardi, G., and Pompa, P. P.
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Materials science ,Silver ,Biomedical Engineering ,Metal Nanoparticles ,Pharmaceutical Science ,Medicine (miscellaneous) ,Nanoparticle ,Bioengineering ,Nanotechnology ,HeLa Cell ,Silver nanoparticle ,HeLa ,Metal Nanoparticle ,Cytosol ,Humans ,General Materials Science ,Chelation ,A549 cell ,biology ,Toxicity ,Cations, Monovalent ,biology.organism_classification ,Lysosome ,Cell culture ,Ion release ,Biophysics ,Molecular Medicine ,Lysosomes ,Intracellular ,HeLa Cells ,Human - Abstract
Toxicity of silver nanoparticles (AgNPs) is supported by many observations in literature, but no mechanism details have been proved yet. Here we confirm and quantify the toxic potential of fully characterized AgNPs in HeLa and A549 cells. Notably, through a specific fluorescent probe, we demonstrate the intracellular release of Ag + ions in living cells after nanoparticle internalization, showing that in-situ particle degradation is promoted by the acidic lysosomal environment. The activation of metallothioneins in response to AgNPs and the possibility to reverse the main toxic pathway by Ag + chelating agents demonstrate a cause/effect relationship between ions and cell death. We propose that endocytosed AgNPs are degraded in the lysosomes and the release of Ag + ions in the cytosol induces cell damages, while ions released in the cell culture medium play a negligible effect. These findings will be useful to develop safer-by-design nanoparticles and proper regulatory guidelines of AgNPs. From the Clinical Editor The authors describe the toxic potential of silver nanoparticles (AgNP) in human cancer cell lines. Cell death following the application of AgNPs is dose-dependent, and it is mostly due to Ag+ ions. Further in vivo studies should be performed to gain a comprehensive picture of AgNP-toxicity in mammals.
- Published
- 2015
35. Toxicity assessment of silica coated iron oxide nanoparticles and biocompatibility improvement by surface engineering
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Antonio Galeone, Pier Paolo Pompa, George C. Anyfantis, Roberto Cingolani, Maria Ada Malvindi, Virgilio Brunetti, Valeria De Matteis, Athanassia Athanassiou, Malvindi, M. A., De Matteis, V., Galeone, A., Brunetti, V., Anyfantis, G. C., Athanassiou, A., Cingolani, R., and Pompa, P. P.
- Subjects
lcsh:Medicine ,Nanoparticle ,medicine.disease_cause ,Toxicology ,Ferric Compounds ,Biochemistry ,chemistry.chemical_compound ,Engineering ,Coated Materials, Biocompatible ,Materials Testing ,Molecular Cell Biology ,Nanotechnology ,lcsh:Science ,health care economics and organizations ,Membrane Potential, Mitochondrial ,Multidisciplinary ,Cell Death ,respiratory system ,Silicon Dioxide ,Endocytosis ,Toxicity ,Cytochemistry ,Medicine ,Iron oxide nanoparticles ,Research Article ,inorganic chemicals ,Biocompatibility ,Silicon dioxide ,Cell Survival ,Surface Properties ,education ,Materials Science ,Surface engineering ,Material by Attribute ,Toxicity Tests ,medicine ,Humans ,Viability assay ,Biology ,Nanomaterials ,Mechanical Engineering ,lcsh:R ,Cell Membrane ,technology, industry, and agriculture ,Culture Media ,chemistry ,Nanoengineering ,Bionanotechnology ,Biophysics ,Nanoparticles ,lcsh:Q ,Reactive Oxygen Species ,Genotoxicity ,DNA Damage ,HeLa Cells - Abstract
We have studied in vitro toxicity of iron oxide nanoparticles (NPs) coated with a thin silica shell (Fe3O4/SiO2 NPs) on A549 and HeLa cells. We compared bare and surface passivated Fe3O4/SiO2 NPs to evaluate the effects of the coating on the particle stability and toxicity. NPs cytotoxicity was investigated by cell viability, membrane integrity, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) assays, and their genotoxicity by comet assay. Our results show that NPs surface passivation reduces the oxidative stress and alteration of iron homeostasis and, consequently, the overall toxicity, despite bare and passivated NPs show similar cell internalization efficiency. We found that the higher toxicity of bare NPs is due to their stronger in-situ degradation, with larger intracellular release of iron ions, as compared to surface passivated NPs. Our results indicate that surface engineering of Fe3O4/SiO2 NPs plays a key role in improving particles stability in biological environments reducing both cytotoxic and genotoxic effects.
- Published
- 2013
36. In vivo nanotoxicology: Toxicoproteomics
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Sabella, S., Kote, S., Maiorano, G., Malvindi, M. A., Valeria De Matteis, Pompa, P. P., Sabella, S., Kote, S., Maiorano, G., Malvindi, M. A., De Matteis, V., and Pompa, P. P.
- Subjects
Quantum dot ,2D-DIGE ,Proteomic ,Nanotoxicity ,Silica nanoparticlcs - Published
- 2013
37. Sustainable nanotechnology and nanomaterials: Environmental and biological applications.
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Singh J, De Matteis V, and Gupta M
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- Nanostructures toxicity, Nanotechnology
- Published
- 2024
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38. Green silver nanoparticles: Prospective nanotools against neurodegenerative cell line model.
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De Matteis V, Martano S, Pellegrino P, Ingrosso C, Costa D, Mazzotta S, Toca-Herrera JL, Rinaldi R, and Cascione M
- Abstract
Neurodegenerative diseases represent an increasingly burdensome challenge of the past decade, primarily driven by the global aging of the population. Ongoing efforts focus on implementing diverse strategies to mitigate the adverse effects of neurodegeneration, with the goal of decelerating the pathology progression. Notably, in recent years, it has emerged that the use of nanoparticles (NPs), particularly those obtained through green chemical processes, could constitute a promising therapeutic approach. Green NPs, exclusively sourced from phytochemicals, are deemed safer compared to NPs synthetized through conventional chemical route. In this study, the effects of green chemistry-derived silver NPs (AgNPs) were assessed in neuroblastoma cells, SHSY-5Y, which are considered a pivotal model for investigating neurodegenerative diseases. Specifically, we used two different concentrations (0.5 and 1 µM) of AgNPs and two time points (24 and 48 h) to evaluate the impact on neuroblastoma cells by observing viability reduction and intracellular calcium production, especially using 1 µM at 48 h. Furthermore, investigation using atomic force microscopy (AFM) unveiled an alteration in Young's modulus due to the reorganization of cortical actin following exposure to green AgNPs. This evidence was further corroborated by confocal microscopy acquisitions as well as coherency and density analyses on actin fibers. Our in vitro findings suggest the potential efficacy of green AgNPs against neurodegeneration; therefore, further in vivo studies are imperative to optimize possible therapeutic protocols., Competing Interests: The authors declare no conflict of interest., (© 2024 The Author(s). Ibrain published by Affiliated Hospital of Zunyi Medical University (AHZMU) and Wiley‐VCH GmbH.)
- Published
- 2024
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39. Shape-Driven Response of Gold Nanoparticles to X-rays.
- Author
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Tarantino S, Capomolla C, Carlà A, Giotta L, Cascione M, Ingrosso C, Scarpa E, Rizzello L, Caricato AP, Rinaldi R, and De Matteis V
- Abstract
Radiotherapy (RT) involves delivering X-ray beams to the tumor site to trigger DNA damage. In this approach, it is fundamental to preserve healthy cells and to confine the X-ray beam only to the malignant cells. The integration of gold nanoparticles (AuNPs) in the X-ray methodology could be considered a powerful tool to improve the efficacy of RT. Indeed, AuNPs have proven to be excellent allies in contrasting tumor pathology upon RT due to their high photoelectric absorption coefficient and unique physiochemical properties. However, an analysis of their physical and morphological reaction to X-ray exposure is necessary to fully understand the AuNPs' behavior upon irradiation before treating the cells, since there are currently no studies on the evaluation of potential NP morphological changes upon specific irradiations. In this work, we synthesized two differently shaped AuNPs adopting two different techniques to achieve either spherical or star-shaped AuNPs. The spherical AuNPs were obtained with the Turkevich-Frens method, while the star-shaped AuNPs (AuNSs) involved a seed-mediated approach. We then characterized all AuNPs with Transmission Electron Microscopy (TEM), Uv-Vis spectroscopy, Dynamic Light Scattering (DLS), zeta potential and Fourier Transform Infrared (FTIR) spectroscopy. The next step involved the treatment of AuNPs with two different doses of X-radiation commonly used in RT, namely 1.8 Gy and 2 Gy, respectively. Following the X-rays' exposure, the AuNPs were further characterized to investigate their possible physicochemical and morphological alterations induced with the X-rays. We found that AuNPs do not undergo any alteration, concluding that they can be safely used in RT treatments. Lastly, the actin rearrangements of THP-1 monocytes treated with AuNPs were also assessed in terms of coherency. This is a key proof to evaluate the possible activation of an immune response, which still represents a big limitation for the clinical translation of NPs.
- Published
- 2023
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40. Silver-Gold Alloy Nanoparticles (AgAu NPs): Photochemical Synthesis of Novel Biocompatible, Bimetallic Alloy Nanoparticles and Study of Their In Vitro Peroxidase Nanozyme Activity.
- Author
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Kshirsagar PG, De Matteis V, Pal S, and Sangaru SS
- Abstract
Facile synthesis of metal nanoparticles with controlled physicochemical properties using environment-friendly reagents can open new avenues in biomedical applications. Nanomaterials with controlled physicochemical properties have opened new prospects for a variety of applications. In the present study, we report a single-step photochemical synthesis of ~5 nm-sized silver (Ag) and gold (Au) nanoparticles (NPs), and Ag-Au alloy nanoparticles using L-tyrosine. The physicochemical and surface properties of both monometallic and bimetallic NPs were investigated by analytical, spectroscopic, and microscopic techniques. Our results also displayed an interaction between L-tyrosine and surface atoms that leads to the formation of AgAu NPs by preventing the growth and aggregation of the NPs. This method efficiently produced monodispersed NPs, with a narrow-sized distribution and good stability in an aqueous solution. The cytotoxicity assessment performed on breast cancer cell lines (MCF-7) revealed that the biofriendly L-tyrosine-capped AgNPs, AuNPs, and bimetallic AgAu NPs were biocompatible. Interestingly, AgAu NPs have also unveiled controlled cytotoxicity, cell viability, and in vitro peroxidase nanozyme activity reliant on metal composition and surface coating.
- Published
- 2023
- Full Text
- View/download PDF
41. Gold and silver nanoparticles in Alzheimer's and Parkinson's diagnostics and treatments.
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Scarpa E, Cascione M, Griego A, Pellegrino P, Moschetti G, and De Matteis V
- Abstract
Neurodegenerative diseases (NDs) impose substantial medical and public health burdens on people worldwide and represent one of the major threats to human health. The prevalence of these age-dependent disorders is dramatically increasing over time, a process intrinsically related to a constantly rising percentage of the elderly population in recent years. Among all the NDs, Alzheimer's and Parkinson's are considered the most debilitating as they cause memory and cognitive loss, as well as severely affecting basic physiological conditions such as the ability to move, speak, and breathe. There is an extreme need for new and more effective therapies to counteract these devastating diseases, as the available treatments are only able to slow down the pathogenic process without really stopping or resolving it. This review aims to elucidate the current nanotechnology-based tools representing a future hope for NDs treatment. Noble metal nano-systems, that is, gold and silver nanoparticles (NPs), have indeed unique physicochemical characteristics enabling them to deliver any pharmacological treatment in a more effective way within the central nervous system. This can potentially make NPs a new hope for reversing the actual therapeutic strategy based on slowing down an irreversible process into a more effective and permanent treatment., Competing Interests: The authors declare no conflicts of interest., (© 2023 The Authors. Ibrain published by Affiliated Hospital of Zunyi Medical University (AHZMU) and Wiley‐VCH GmbH.)
- Published
- 2023
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- View/download PDF
42. Cancer Treatment Using Different Shapes of Gold-Based Nanomaterials in Combination with Conventional Physical Techniques.
- Author
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Tarantino S, Caricato AP, Rinaldi R, Capomolla C, and De Matteis V
- Abstract
The conventional methods of cancer treatment and diagnosis, such as radiotherapy, chemotherapy, and computed tomography, have developed a great deal. However, the effectiveness of such methods is limited to the possible failure or collateral effects on the patients. In recent years, nanoscale materials have been studied in the field of medical physics to develop increasingly efficient methods to treat diseases. Gold nanoparticles (AuNPs), thanks to their unique physicochemical and optical properties, were introduced to medicine to promote highly effective treatments. Several studies have confirmed the advantages of AuNPs such as their biocompatibility and the possibility to tune their shapes and sizes or modify their surfaces using different chemical compounds. In this review, the main properties of AuNPs are analyzed, with particular focus on star-shaped AuNPs. In addition, the main methods of tumor treatment and diagnosis involving AuNPs are reviewed.
- Published
- 2023
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43. Nanoparticle delivery through the BBB in central nervous system tuberculosis.
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Griego A, Scarpa E, De Matteis V, and Rizzello L
- Abstract
Recent advances in Nanotechnology have revolutionized the production of materials for biomedical applications. Nowadays, there is a plethora of nanomaterials with potential for use towards improvement of human health. On the other hand, very little is known about how these materials interact with biological systems, especially at the nanoscale level, mainly because of the lack of specific methods to probe these interactions. In this review, we will analytically describe the journey of nanoparticles (NPs) through the brain, starting from the very first moment upon injection. We will preliminarily provide a brief overlook of the physicochemical properties of NPs. Then, we will discuss how these NPs interact with the body compartments and biological barriers, before reaching the blood-brain barrier (BBB), the last gate guarding the brain. Particular attention will be paid to the interaction with the biomolecular, the bio-mesoscopic, the (blood) cellular, and the tissue barriers, with a focus on the BBB. This will be framed in the context of brain infections, especially considering central nervous system tuberculosis (CNS-TB), which is one of the most devastating forms of human mycobacterial infections. The final aim of this review is not a collection, nor a list, of current literature data, as it provides the readers with the analytical tools and guidelines for the design of effective and rational NPs for delivery in the infected brain., Competing Interests: Prof. Loris Rizzello is the associate editor of Ibrian; he is not involved in peer review. The remaining authors declare no conflict of interest., (© 2023 The Authors. Ibrain published by Affiliated Hospital of Zunyi Medical University (AHZMU) and Wiley‐VCH GmbH.)
- Published
- 2023
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44. Investigation of the Effects of Pulse-Atomic Force Nanolithography Parameters on 2.5D Nanostructures' Morphology.
- Author
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Pellegrino P, Farella I, Cascione M, De Matteis V, Bramanti AP, Della Torre A, Quaranta F, and Rinaldi R
- Abstract
In recent years, Atomic Force Microscope (AFM)-based nanolithography techniques have emerged as a very powerful approach for the machining of countless types of nanostructures. However, the conventional AFM-based nanolithography methods suffer from low efficiency, low rate of patterning, and high complexity of execution. In this frame, we first developed an easy and effective nanopatterning technique, termed Pulse-Atomic Force Lithography (P-AFL), with which we were able to pattern 2.5D nanogrooves on a thin polymer layer. Indeed, for the first time, we patterned nanogrooves with either constant or varying depth profiles, with sub-nanometre resolution, high accuracy, and reproducibility. In this paper, we present the results on the investigation of the effects of P-AFL parameters on 2.5D nanostructures' morphology. We considered three main P-AFL parameters, i.e., the pulse's amplitude (setpoint), the pulses' width, and the distance between the following indentations (step), and we patterned arrays of grooves after a precise and well-established variation of the aforementioned parameters. Optimizing the nanolithography process, in terms of patterning time and nanostructures quality, we realized unconventional shape nanostructures with high accuracy and fidelity. Finally, a scanning electron microscope was used to confirm that P-AFL does not induce any damage on AFM tips used to pattern the nanostructures.
- Published
- 2022
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45. Pile-Ups Formation in AFM-Based Nanolithography: Morpho-Mechanical Characterization and Removal Strategies.
- Author
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Pellegrino P, Farella I, Cascione M, De Matteis V, Bramanti AP, Vincenti L, Della Torre A, Quaranta F, and Rinaldi R
- Abstract
In recent decades, great efforts have been made to develop innovative, effective, and accurate nanofabrication techniques stimulated by the growing demand for nanostructures. Nowadays, mechanical tip-based emerged as the most promising nanolithography technique, allowing the pattern of nanostructures with a sub-nanometer resolution, high reproducibility, and accuracy. Unfortunately, these nanostructures result in contoured pile-ups that could limit their use and future integration into high-tech devices. The removal of pile-ups is still an open challenge. In this perspective, two different AFM-based approaches, i.e., Force Modulation Mode imaging and force-distance curve analysis, were used to characterize the structure of pile-ups at the edges of nanogrooves patterned on PMMA substrate by means of Pulse-Atomic Force Lithography. Our experimental results showed that the material in pile-ups was less stiff than the pristine polymer. Based on this evidence, we have developed an effective strategy to easily remove pile-ups, preserving the shape and the morphology of nanostructures.
- Published
- 2022
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46. Inorganic Nanomaterials versus Polymer-Based Nanoparticles for Overcoming Neurodegeneration.
- Author
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Martano S, De Matteis V, Cascione M, and Rinaldi R
- Abstract
Neurodegenerative disorders (NDs) affect a great number of people worldwide and also have a significant socio-economic impact on the aging population. In this context, nanomedicine applied to neurological disorders provides several biotechnological strategies and nanoformulations that improve life expectancy and the quality of life of patients affected by brain disorders. However, available treatments are limited by the presence of the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (B-CSFB). In this regard, nanotechnological approaches could overcome these obstacles by updating various aspects (e.g., enhanced drug-delivery efficiency and bioavailability, BBB permeation and targeting the brain parenchyma, minimizing side effects). The aim of this review is to carefully explore the key elements of different neurological disorders and summarize the available nanomaterials applied for neurodegeneration therapy looking at several types of nanocarriers. Moreover, nutraceutical-loaded nanoparticles (NPs) and synthesized NPs using green approaches are also discussed underling the need to adopt eco-friendly procedures with a low environmental impact. The proven antioxidant properties related to several natural products provide an interesting starting point for developing efficient and green nanotools useful for neuroprotection.
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- 2022
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47. Impact of Nanomaterials in Biological Systems and Applications in Nanomedicine Field.
- Author
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De Matteis V, Cascione M, and Leporatti S
- Abstract
The increasingly widespread use of engineered nanomaterials in many applications increases the need to understand the mechanisms behind their toxicity [...].
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- 2022
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48. AFM Characterization of Halloysite Clay Nanocomposites' Superficial Properties: Current State-of-the-Art and Perspectives.
- Author
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Cascione M, De Matteis V, Persano F, and Leporatti S
- Abstract
Natural halloysite clay nanotubes (HNTs) are versatile inorganic reinforcing materials for creating hybrid composites. Upon doping HNTs with polymers, coating, or loading them with bioactive molecules, the production of novel nanocomposites is possible, having specific features for several applications. To investigate HNTs composites nanostructures, AFM is a very powerful tool since it allows for performing nano-topographic and morpho-mechanical measurements in any environment (air or liquid) without treatment of samples, like electron microscopes require. In this review, we aimed to provide an overview of recent AFM investigations of HNTs and HNT nanocomposites for unveiling hidden characteristics inside them envisaging future perspectives for AFM as a smart device in nanomaterials characterization.
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- 2022
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49. Pulse-Atomic Force Lithography: A Powerful Nanofabrication Technique to Fabricate Constant and Varying-Depth Nanostructures.
- Author
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Pellegrino P, Bramanti AP, Farella I, Cascione M, De Matteis V, Della Torre A, Quaranta F, and Rinaldi R
- Abstract
The widespread use of nanotechnology in different application fields, resulting in the integration of nanostructures in a plethora of devices, has addressed the research toward novel and easy-to-setup nanofabrication techniques to realize nanostructures with high spatial resolution and reproducibility. Owing to countless applications in molecular electronics, data storage, nanoelectromechanical, and systems for the Internet of Things, in recent decades, the scientific community has focused on developing methods suitable for nanopattern polymers. To this purpose, Atomic Force Microscopy-based nanolithographic techniques are effective methods that are relatively less complex and inexpensive than equally resolute and accurate techniques, such as Electron Beam lithography and Focused Ion Beam lithography. In this work, we propose an evolution of nanoindentation, named Pulse-Atomic Force Microscopy, to obtain continuous structures with a controlled depth profile, either constant or variable, on a polymer layer. Due to the modulation of the characteristics of voltage pulses fed to the AFM piezo-scanner and distance between nanoindentations, it was possible to indent sample surface with high spatial control and fabricate highly resolved 2.5D nanogrooves. That is the real strength of the proposed technique, as no other technique can achieve similar results in tailor-made graded nanogrooves without the need for additional manufacturing steps.
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- 2022
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50. High Doses of Silica Nanoparticles Obtained by Microemulsion and Green Routes Compromise Human Alveolar Cells Morphology and Stiffness Differently.
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De Matteis V, Cascione M, De Luca A, Manno DE, and Rinaldi R
- Abstract
Among all the inorganic nanomaterials used in commercial products, industry, and medicine, the amorphous silica nanoparticles (SiO
2 NPs) appeared to be often tolerated in living organisms. However, despite several toxicity studies, some concerns about the exposure to high doses of SiO2 NPs with different sizes were raised. Then, we used the microemulsion method to obtain stable SiO2 NPs having different sizes (110 nm, 50 nm, and 25 nm). In addition, a new one-pot green synthetic route using leaves extract of Laurus nobilis was performed, obtaining monodispersed ultrasmall SiO2 NPs without the use of dangerous chemicals. The NPs achieved by microemulsion were further functionalized with amino groups making the NPs surface positively charged. Then, high doses of SiO2 NPs (1 mg/mL and 3 mg/mL) achieved from the two routes, having different sizes and surface charges, were used to assess their impact on human alveolar cells (A549), being the best cell model mimicking the inhalation route. Cell viability and caspase-3 induction were analyzed as well as the cellular uptake, obtaining that the smallest (25 nm) and positive-charged NPs were more able to induce cytotoxicity, reaching values of about 60% of cell death. Surprisingly, cells incubated with green SiO2 NPs did not show strong toxicity, and 70% of them remained vital. This result was unusual for ultrasmall nanoobjects, generally highly toxic. The actin reorganization, nuclear morphology alteration, and cell membrane elasticity analyses confirmed the trend achieved from the biological assays. The obtained data demonstrate that the increase in cellular softness, i.e., the decrease in Young's modulus, could be associated with the smaller and positive NPs, recording values of about 3 kPa. On the contrary, green NPs triggered a slight decrease of stiffness values (c.a. 6 kPa) compared to the untreated cells (c.a. 8 kPa). As the softer cells were implicated in cancer progression and metastasization, this evidence strongly supported the idea of a link between the cell elasticity and physicochemical properties of NPs that, in turn, influenced the interaction with the cell membrane. Thus, the green SiO2 NPs compromised cells to a lesser extent than the other SiO2 NPs types. In this scenario, the elasticity evaluation could be an interesting tool to understand the toxicity of NPs with the aim of predicting some pathological phenomena associated with their exposure., Competing Interests: The authors declare no conflicts of interest., (Copyright © 2022 Valeria De Matteis et al.)- Published
- 2022
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