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21 p.-8 fig.-1 tab., The coordination of cell division with stress response is essential for maintaining genome stability in plant meristems. Proteins involved in pre-mRNA splicing are important for these processes in animal and human cells. Based on its homology to the splicing factor SART1, which is implicated in the control of cell division and genome stability in human cells, we analyzed if MDF has similar functions in plants. We found that MDF associates with U4/U6.U5 tri-snRNP proteins and is essential for correct splicing of 2,037 transcripts. Loss of MDF function leads to cell division defects and cell death in meristems and was associated with up-regulation of stress-induced genes and down-regulation of mitotic regulators. In addition, the mdf-1 mutant is hypersensitive to DNA damage treatment supporting its role in coordinating stress response with cell division. Our analysis of a dephosphomutant of MDF suggested how its protein activity might be controlled. Our work uncovers the conserved function of a plant splicing factor and provides novel insight into the interplay of pre-mRNA processing and genome stability in plants., This work was funded by the Deutsche Forschungsgemeinschaft (DFG) grant to M Weingartner (WE4506/6-1). C de Luxán-Hernández was recipient of a PhD fellowship from the University of Hamburg. In addition, funding was received from the Spanish Ministry of Science and Innovation to J Salinas (PID2019-106987RB-I00/AEI/10.13039/5011033), from the Spanish Ministry of Science and Innovation to E Tranque (FPI contract), from the Czech Academy of Science, Deutsche Akademische Austauschdienst, and Bundesministeriums für Bildung und Forschung (BMBF) (CAS Mobility DAAD-19-04 and CAS Mobility DAAD-21-07) to M Weingartner and P Binarova.