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4. Three‐dimensional (3D) polylactic acid gradient scaffold to study the behavior of osteosarcoma cells under dynamic conditions.

Author

De Luca, Angela, Capuana, Elisa, Carbone, Camilla, Raimondi, Lavinia, Carfì Pavia, Francesco, Brucato, Valerio, La Carrubba, Vincenzo, and Giavaresi, Gianluca

Abstract

This study adopts an in vitro method to recapitulate the behavior of Saos‐2 cells, using a system composed of a perfusion bioreactor and poly‐L‐lactic acid (PLLA) scaffold fabricated using the low‐cost thermally‐induced phase separation (TIPS) technique. Four distinct scaffold morphologies with different pore sizes were fabricated, characterized by Scanning electron microscopy and micro‐CT analysis and tested with osteosarcoma cells under static and dynamic environments to identify the best morphology for cellular growth. In order to accomplish this purpose, cell growth and matrix deposition of the Saos‐2 osteosarcoma cell line were assessed using Picogreen and OsteoImage assays. The obtained data allowed us to identify the morphology that better promotes Saos‐2 cellular activity in static and dynamic conditions. These findings provided valuable insights into scaffold design and fabrication strategies, emphasizing the importance of the dynamic culture to recreate an appropriate 3D osteosarcoma model. Remarkably, the gradient scaffold exhibits promise for osteosarcoma applications, offering the potential for targeted tissue engineering approaches. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Magnesium Alloys in Orthopedics: A Systematic Review on Approaches, Coatings and Strategies to Improve Biocompatibility, Osteogenic Properties and Osteointegration Capabilities

Author

Giavaresi, Gianluca, primary, Bellavia, Daniele, additional, De Luca, Angela De, additional, Costa, Viviana, additional, Raimondi, Lavinia, additional, Cordaro, Aurora, additional, Sartori, Maria, additional, Terrando, Silvio, additional, Toscano, Angelo, additional, Pignatti, Giovanni, additional, and Fini, Milena, additional

Published
2023
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8. Bactericidal Activity of Silver-Doped Chitosan Coatings via Electrophoretic Deposition on Ti6Al4V Additively Manufactured Substrates

Author

Ghalayani Esfahani, Arash, primary, Sartori, Maria, additional, Bregoli, Chiara, additional, Fiocchi, Jacopo, additional, Biffi, Carlo Alberto, additional, Tuissi, Ausonio, additional, Giavaresi, Gianluca, additional, Presentato, Alessandro, additional, Alduina, Rosa, additional, De Luca, Angela, additional, Cabrini, Alessia, additional, De Capitani, Cristina, additional, Fini, Milena, additional, Gruppioni, Emanuele, additional, Lavorgna, Marino, additional, and Ronca, Alfredo, additional

Published
2023
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11. Multiparametric Dynamic Ultrasound Approach for Differential Diagnosis of Primary Liver Tumors

Author

Ainora, Maria Elena, primary, Cerrito, Lucia, additional, Liguori, Antonio, additional, Mignini, Irene, additional, De Luca, Angela, additional, Galasso, Linda, additional, Garcovich, Matteo, additional, Riccardi, Laura, additional, Ponziani, Francesca, additional, Santopaolo, Francesco, additional, Pompili, Maurizio, additional, Gasbarrini, Antonio, additional, and Zocco, Maria Assunta, additional

Published
2023
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12. Investigating the Differential Circulating microRNA Expression in Adolescent Females with Severe Idiopathic Scoliosis: A Proof-of-Concept Observational Clinical Study.

Author

Raimondi, Lavinia, De Luca, Angela, Gallo, Alessia, Perna, Fabrizio, Cuscino, Nicola, Cordaro, Aurora, Costa, Viviana, Bellavia, Daniele, Faldini, Cesare, Scilabra, Simone Dario, Giavaresi, Gianluca, and Toscano, Angelo

Subjects
*GENE expression, *TEENAGE girls, *ADOLESCENT idiopathic scoliosis, *MESENCHYMAL stem cell differentiation, *BONE density, *ADOLESCENCE, *BONE regeneration, *FEMALES
Abstract

Adolescent Idiopathic Scoliosis (AIS) is the most common form of three-dimensional spinal disorder in adolescents between the ages of 10 and 18 years of age, most commonly diagnosed in young women when severe disease occurs. Patients with AIS are characterized by abnormal skeletal growth and reduced bone mineral density. The etiology of AIS is thought to be multifactorial, involving both environmental and genetic factors, but to date, it is still unknown. Therefore, it is crucial to further investigate the molecular pathogenesis of AIS and to identify biomarkers useful for predicting curve progression. In this perspective, the relative abundance of a panel of microRNAs (miRNAs) was analyzed in the plasma of 20 AIS patients and 10 healthy controls (HC). The data revealed a significant group of circulating miRNAs dysregulated in AIS patients compared to HC. Further bioinformatic analyses evidenced a more restricted expression of some miRNAs exclusively in severe AIS females. These include some members of the miR-30 family, which are considered promising regulators for treating bone diseases. We demonstrated circulating extracellular vesicles (EVs) from severe AIS females contained miR-30 family members and decreased the osteogenic differentiation of mesenchymal stem cells. Proteomic analysis of EVs highlighted the expression of proteins associated with orthopedic disease. This study provides preliminary evidence of a miRNAs signature potentially associated with severe female AIS and suggests the corresponding vesicular component may affect cellular mechanisms crucial in AIS, opening the scenario for in-depth studies on prognostic differences related to gender and grade. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Magnesium Alloys in Orthopedics: A Systematic Review on Approaches, Coatings and Strategies to Improve Biocompatibility, Osteogenic Properties and Osteointegration Capabilities.

Author

Giavaresi, Gianluca, Bellavia, Daniele, De Luca, Angela, Costa, Viviana, Raimondi, Lavinia, Cordaro, Aurora, Sartori, Maria, Terrando, Silvio, Toscano, Angelo, Pignatti, Giovanni, and Fini, Milena

Subjects
MAGNESIUM alloys, OSSEOINTEGRATION, ORTHOPEDIC apparatus, ORTHOPEDICS, BONE regeneration, SURFACE coatings
Abstract

There is increasing interest in using magnesium (Mg) alloy orthopedic devices because of their mechanical properties and bioresorption potential. Concerns related to their rapid degradation have been issued by developing biodegradable micro- and nanostructured coatings to enhance corrosion resistance and limit the release of hydrogen during degradation. This systematic review based on four databases (PubMed®, Embase, Web of Science™ and ScienceDirect®) aims to present state-of-the-art strategies, approaches and materials used to address the critical factors currently impeding the utilization of Mg alloy devices. Forty studies were selected according to PRISMA guidelines and specific PECO criteria. Risk of bias assessment was conducted using OHAT and SYRCLE tools for in vitro and in vivo studies, respectively. Despite limitations associated with identified bias, the review provides a comprehensive analysis of preclinical in vitro and in vivo studies focused on manufacturing and application of Mg alloys in orthopedics. This attests to the continuous evolution of research related to Mg alloy modifications (e.g., AZ91, LAE442 and WE43) and micro- and nanocoatings (e.g., MAO and MgF2), which are developed to improve the degradation rate required for long-term mechanical resistance to loading and excellent osseointegration with bone tissue, thereby promoting functional bone regeneration. Further research is required to deeply verify the safety and efficacy of Mg alloys. [ABSTRACT FROM AUTHOR]

Published
2024
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14. Osteoarthritis in the Elderly Population: Preclinical Evidence of Nutrigenomic Activities of Flavonoids.

Author

Naselli, Flores, Bellavia, Daniele, Costa, Viviana, De Luca, Angela, Raimondi, Lavinia, Giavaresi, Gianluca, and Caradonna, Fabio

Abstract

Osteoarthritis (OA) is a degenerative joint disease that is age-related and progressive. It causes the destruction of articular cartilage and underlying bone, often aggravated by inflammatory processes and oxidative stresses. This pathology impairs the quality of life of the elderly, causing pain, reduced mobility, and functional disabilities, especially in obese patients. Phytochemicals with anti-inflammatory and antioxidant activities may be used for long-term treatment of OA, either in combination with current anti-inflammatories and painkillers, or as an alternative to other products such as glucosamine and chondroitin, which improve cartilage structure and elasticity. The current systematic review provides a comprehensive understanding of the use of flavonoids. It highlights chondrocyte, cartilage, and subchondral bone activities, with a particular focus on their nutrigenomic effects. The molecular mechanisms of these molecules demonstrate how they can be used for the prevention and treatment of OA in the elderly population. However, clinical trials are still needed for effective use in clinical practice. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Figure S6 from A Four-Chemokine Signature Is Associated with a T-cell–Inflamed Phenotype in Primary and Metastatic Pancreatic Cancer

Author

Romero, Joan M., primary, Grünwald, Barbara, primary, Jang, Gun-Ho, primary, Bavi, Prashant P., primary, Jhaveri, Aaditeya, primary, Masoomian, Mehdi, primary, Fischer, Sandra E., primary, Zhang, Amy, primary, Denroche, Robert E., primary, Lungu, Ilinca M., primary, De Luca, Angela, primary, Bartlett, John M.S., primary, Xu, Jing, primary, Li, Niandong, primary, Dhaliwal, Sharon, primary, Liang, Sheng-Ben, primary, Chadwick, Dianne, primary, Vyas, Foram, primary, Bronsert, Peter, primary, Khokha, Rama, primary, McGaha, Tracy L., primary, Notta, Faiyaz, primary, Ohashi, Pamela S., primary, Done, Susan J., primary, O'Kane, Grainne M., primary, Wilson, Julie M., primary, Knox, Jennifer J., primary, Connor, Ashton, primary, Wang, Yifan, primary, Zogopoulos, George, primary, and Gallinger, Steven, primary

Published
2023
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16. Table S3 from A Four-Chemokine Signature Is Associated with a T-cell–Inflamed Phenotype in Primary and Metastatic Pancreatic Cancer

Author

Romero, Joan M., primary, Grünwald, Barbara, primary, Jang, Gun-Ho, primary, Bavi, Prashant P., primary, Jhaveri, Aaditeya, primary, Masoomian, Mehdi, primary, Fischer, Sandra E., primary, Zhang, Amy, primary, Denroche, Robert E., primary, Lungu, Ilinca M., primary, De Luca, Angela, primary, Bartlett, John M.S., primary, Xu, Jing, primary, Li, Niandong, primary, Dhaliwal, Sharon, primary, Liang, Sheng-Ben, primary, Chadwick, Dianne, primary, Vyas, Foram, primary, Bronsert, Peter, primary, Khokha, Rama, primary, McGaha, Tracy L., primary, Notta, Faiyaz, primary, Ohashi, Pamela S., primary, Done, Susan J., primary, O'Kane, Grainne M., primary, Wilson, Julie M., primary, Knox, Jennifer J., primary, Connor, Ashton, primary, Wang, Yifan, primary, Zogopoulos, George, primary, and Gallinger, Steven, primary

Published
2023
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18. Multiparametric Dynamic Ultrasound Approach for Differential Diagnosis of Primary Liver Tumors

Author

Ainora, Maria Elena, Cerrito, Lucia, Liguori, Antonio, Mignini, Irene, De Luca, Angela, Galasso, Linda, Garcovich, Matteo, Riccardi, Laura, Ponziani, Francesca Romana, Santopaolo, Francesco, Pompili, Maurizio, Gasbarrini, Antonio, Zocco, Maria Assunta, Ponziani, Francesca (ORCID:0000-0002-5924-6238), Pompili, Maurizio (ORCID:0000-0001-6699-7980), Gasbarrini, Antonio (ORCID:0000-0002-7278-4823), Zocco, Maria Assunta (ORCID:0000-0002-0814-9542), Ainora, Maria Elena, Cerrito, Lucia, Liguori, Antonio, Mignini, Irene, De Luca, Angela, Galasso, Linda, Garcovich, Matteo, Riccardi, Laura, Ponziani, Francesca Romana, Santopaolo, Francesco, Pompili, Maurizio, Gasbarrini, Antonio, Zocco, Maria Assunta, Ponziani, Francesca (ORCID:0000-0002-5924-6238), Pompili, Maurizio (ORCID:0000-0001-6699-7980), Gasbarrini, Antonio (ORCID:0000-0002-7278-4823), and Zocco, Maria Assunta (ORCID:0000-0002-0814-9542)

Abstract

A correct differentiation between hepatocellular carcinoma (HCC) and intracellular cholangiocarcinoma (ICC) is essential for clinical management and prognostic prediction. However, non-invasive differential diagnosis between HCC and ICC remains highly challenging. Dynamic contrast-enhanced ultrasound (D-CEUS) with standardized software is a valuable tool in the diagnostic approach to focal liver lesions and could improve accuracy in the evaluation of tumor perfusion. Moreover, the measurement of tissue stiffness could add more information concerning tumoral environment. To explore the diagnostic performance of multiparametric ultrasound (MP-US) in differentiating ICC from HCC. Our secondary aim was to develop an US score for distinguishing ICC and HCC. Between January 2021 and September 2022 consecutive patients with histologically confirmed HCC and ICC were enrolled in this prospective monocentric study. A complete US evaluation including B mode, D-CEUS and shear wave elastography (SWE) was performed in all patients and the corresponding features were compared between the tumor entities. For better inter-individual comparability, the blood volume-related D-CEUS parameters were analyzed as a ratio between lesions and surrounding liver parenchyma. Univariate and multivariate regression analysis was performed to select the most useful independent variables for the differential diagnosis between HCC and ICC and to establish an US score for non-invasive diagnosis. Finally, the diagnostic performance of the score was evaluated by receiver operating characteristic (ROC) curve analysis. A total of 82 patients (mean age ± SD, 68 ± 11 years, 55 men) were enrolled, including 44 ICC and 38 HCC. No statistically significant differences in basal US features were found between HCC and ICC. Concerning D-CEUS, blood volume parameters (peak intensity, PE; area under the curve, AUC; and wash-in rate, WiR) showed significantly higher values in the HCC group, but PE was the only inde

Published
2023

19. Bactericidal Activity of Silver-Doped Chitosan Coatings via Electrophoretic Deposition on Ti 6 Al 4 V Additively Manufactured Substrates.

Author

Ghalayani Esfahani, Arash, Sartori, Maria, Bregoli, Chiara, Fiocchi, Jacopo, Biffi, Carlo Alberto, Tuissi, Ausonio, Giavaresi, Gianluca, Presentato, Alessandro, Alduina, Rosa, De Luca, Angela, Cabrini, Alessia, De Capitani, Cristina, Fini, Milena, Gruppioni, Emanuele, Lavorgna, Marino, and Ronca, Alfredo

Subjects
ELECTROPHORETIC deposition, CHITOSAN, COMPOSITE coating, FINGER injuries, ARTIFICIAL hip joints, MEDICAL equipment, SILVER
Abstract

Prosthetic reconstruction can serve as a feasible alternative, delivering both functional and aesthetic benefits to individuals with hand and finger injuries, frequent causes of emergency room visits. Implant-related infections pose significant challenges in arthroplasty and osteosynthesis procedures, contributing to surgical failures. As a potential solution to this challenge, this study developed a new class of silver (Ag)-doped chitosan (CS) coatings via electrophoretic deposition (EPD) on osseointegrated prostheses for infection therapy. These coatings were successfully applied to additively manufactured Ti6Al4V ELI samples. In the initial phase, the feasibility of the composite coating was assessed using the Thermogravimetric Analysis (TGA) and Attenuated Total Reflection (ATR) techniques. The optimized structures exhibited impressive water uptake in the range of 300–360%. Codeposition with an antibacterial agent proved effective, and scanning electron microscopy (SEM) was used to examine the coating morphology. Biologically, CS coatings demonstrated cytocompatibility when in direct contact with a fibroblast cell line (L929) after 72 h. When exposed to the Staphylococcus epidermidis strain (ATCC 12228), these coatings inhibited bacterial growth and biofilm formation within 24 h. These findings underscore the significant potential of this approach for various applications, including endoprostheses like hip implants, internal medical devices, and transcutaneous prostheses such as osseointegrated limb prosthetics for upper and lower extremities. [ABSTRACT FROM AUTHOR]

Published
2023
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23. Gut Dysbiosis and Fecal Calprotectin Predict Response to Immune Checkpoint Inhibitors in Patients With Hepatocellular Carcinoma

Author

Ponziani, Francesca Romana, De Luca, Angela, Picca, Anna, Marzetti, Emanuele, Petito, Valentina, Del Chierico, Federica, Reddel, Sofia, Paroni Sterbini, Francesco, Sanguinetti, Maurizio, Putignani, Lorenza, Gasbarrini, Antonio, Pompili, Maurizio, Ponziani, Francesca Romana, De Luca, Angela, Picca, Anna, Marzetti, Emanuele, Petito, Valentina, Del Chierico, Federica, Reddel, Sofia, Paroni Sterbini, Francesco, Sanguinetti, Maurizio, Putignani, Lorenza, Gasbarrini, Antonio, and Pompili, Maurizio

Abstract

The gut microbiota is a well-known prognostic factor and a modulator of treatment sensitivity in patients with cancers treated with immune checkpoint inhibitors. However, data on hepatocellular carcinoma (HCC) are lacking. This study aimed to evaluate the prognostic role of the gut microbiota and changes produced by immunotherapy on the intestinal environment in patients with cirrhosis and HCC. Eleven patients treated with Tremelimumab and/or Durvalumab were included in the analysis. All study participants underwent gut microbiota profiling, quantification of fecal calprotectin, serum levels of zonulin-1, lipopolysaccharide binding protein (LBP), and programmed death-ligand 1 (PD-L1) at baseline and at each treatment cycle until the third cycle, then every three cycles until treatment discontinuation or last visit. The 6 patients who achieved disease control (DC) showed lower pretreatment fecal calprotectin (median, 12.5; interquartile range [IQR], 5-29 vs. median, 116; IQR, 59-129 µg/g; P = 0.047) and PD-L1 serum levels (median, 0.08; IQR, 0.07-0.09 vs. median, 1.04; IQR, 0.17-1.95 ng/mL; P = 0.02) than nonresponders. The relative abundance of Akkermansia (log2 fold change [FC], 2.72; adjusted P [Padj] = 0.012) was increased, whereas that of Enterobacteriaceae (log2 FC, −2.34; Padj = 0.04) was reduced in the DC group. During treatment, fecal calprotectin showed a temporal evolution opposite to the Akkermansia to Enterobacteriaceae ratio and gut microbiota alpha diversity, but similar to zonulin-1 and LBP. Bifidobacterium had a stable behavior in patients with a long follow-up, while Akkermansia was more variable. Akkermansia and Bifidobacterium showed similar temporal patterns and causative relationships with Prevotella, Veillonella, Ruminococcus, Roseburia, Lachnospira, Faecalibacterium, and Clostridium. Conclusion: A favorable composition of the gut microbiota and low intestinal inflammation are associated with achieving DC. The intestinal environment changes dyn

Published
2022
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24. Timing Expression of miR203a-3p during OA Disease: Preliminary In Vitro Evidence.

Author

Costa, Viviana, De Fine, Marcello, Raimondi, Lavinia, Bellavia, Daniele, Cordaro, Aurora, Carina, Valeria, Alessandro, Riccardo, Pignatti, Giovanni, Fini, Milena, Giavaresi, Gianluca, and De Luca, Angela

Subjects
GENE expression, OSTEOARTHRITIS, INFLAMMATION, DEGENERATION (Pathology), BONE diseases, MARINE toxins
Abstract

Osteoarthritis (OA) is a degenerative bone disease that involves the microenvironment and macroenvironment of joints. Progressive joint tissue degradation and loss of extracellular matrix elements, together with different grades of inflammation, are important hallmarks of OA disease. Therefore, the identification of specific biomarkers to distinguish the stages of disease becomes a primary necessity in clinical practice. To this aim, we investigated the role of miR203a-3p in OA progression starting from the evidence obtained by osteoblasts isolated from joint tissues of OA patients classified according to different Kellgren and Lawrence (KL) grading (KL ≤ 3 and KL > 3) and hMSCs treated with IL-1β. Through qRT-PCR analysis, it was found that osteoblasts (OBs) derived from the KL ≤ 3 group expressed high levels of miR203a-3p and low levels of ILs compared with those of OBs derived from the KL > 3 group. The stimulation with IL-1β improved the expression of miR203a-3p and the methylation of the IL-6 promoter gene, favoring an increase in relative protein expression. The gain and loss of function studies showed that the transfection with miR203a-3p inhibitor alone or in co-treatments with IL-1β was able to induce the expression of CX-43 and SP-1 and to modulate the expression of TAZ, in OBs derived from OA patients with KL ≤ 3 compared with KL > 3. These events, confirmed also by qRT-PCR analysis, Western blot, and ELISA assay performed on hMSCs stimulated with IL-1β, supported our hypothesis about the role of miR203a-3p in OA progression. The results suggested that during the early stage, miR203a-3p displayed a protective role reducing the inflammatory effects on CX-43, SP-1, and TAZ. During the OA progression the downregulation of miR203a-3p and consequently the upregulation of CX-43/SP-1 and TAZ expression improved the inflammatory response and the reorganization of the cytoskeleton. This role led to the subsequent stage of the disease, where the aberrant inflammatory and fibrotic responses determined the destruction of the joint. [ABSTRACT FROM AUTHOR]

Published
2023
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26. Gut Dysbiosis and Fecal Calprotectin Predict Response to Immune Checkpoint Inhibitors in Patients With Hepatocellular Carcinoma

Author

Ponziani, Francesca Romana, primary, De Luca, Angela, additional, Picca, Anna, additional, Marzetti, Emanuele, additional, Petito, Valentina, additional, Del Chierico, Federica, additional, Reddel, Sofia, additional, Paroni Sterbini, Francesco, additional, Sanguinetti, Maurizio, additional, Putignani, Lorenza, additional, Gasbarrini, Antonio, additional, and Pompili, Maurizio, additional

Published
2022
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27. Potential Anti-Metastatic Role of the Novel miR-CT3 in Tumor Angiogenesis and Osteosarcoma Invasion

Author

Raimondi, Lavinia, primary, Gallo, Alessia, additional, Cuscino, Nicola, additional, De Luca, Angela, additional, Costa, Viviana, additional, Carina, Valeria, additional, Bellavia, Daniele, additional, Bulati, Matteo, additional, Alessandro, Riccardo, additional, Fini, Milena, additional, Conaldi, Pier Giulio, additional, and Giavaresi, Gianluca, additional

Published
2022
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28. Terpenoid treatment in osteoporosis: this is where we have come in research

Author

Bellavia, Daniele, primary, Caradonna, Fabio, additional, Dimarco, Eufrosina, additional, Costa, Viviana, additional, Carina, Valeria, additional, De Luca, Angela, additional, Raimondi, Lavinia, additional, Gentile, Carla, additional, Alessandro, Riccardo, additional, Fini, Milena, additional, and Giavaresi, Gianluca, additional

Published
2021
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29. Non-flavonoid polyphenols in osteoporosis: preclinical evidence

Author

Bellavia, Daniele, primary, Caradonna, Fabio, additional, Dimarco, Eufrosina, additional, Costa, Viviana, additional, Carina, Valeria, additional, De Luca, Angela, additional, Raimondi, Lavinia, additional, Fini, Milena, additional, Gentile, Carla, additional, and Giavaresi, Gianluca, additional

Published
2021
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33. How miR-31-5p and miR-33a-5p Regulates SP1/CX43 Expression in Osteoarthritis Disease: Preliminary Insights

Author

Costa, Viviana, primary, De Fine, Marcello, additional, Carina, Valeria, additional, Conigliaro, Alice, additional, Raimondi, Lavinia, additional, De Luca, Angela, additional, Bellavia, Daniele, additional, Salamanna, Francesca, additional, Alessandro, Riccardo, additional, Pignatti, Giovanni, additional, Fini, Milena, additional, and Giavaresi, Gianluca, additional

Published
2021
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36. Non-Coding RNAs in Multiple Myeloma Bone Disease Pathophysiology

Author

Raimondi, Lavinia, primary, De Luca, Angela, additional, Giavaresi, Gianluca, additional, Raimondo, Stefania, additional, Gallo, Alessia, additional, Taiana, Elisa, additional, Alessandro, Riccardo, additional, Rossi, Marco, additional, Neri, Antonino, additional, Viglietto, Giuseppe, additional, and Amodio, Nicola, additional

Published
2020
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37. Multiple Myeloma-Derived Extracellular Vesicles Induce Osteoclastogenesis through the Activation of the XBP1/IRE1α Axis

Author

Raimondi, Lavinia, primary, De Luca, Angela, additional, Fontana, Simona, additional, Amodio, Nicola, additional, Costa, Viviana, additional, Carina, Valeria, additional, Bellavia, Daniele, additional, Raimondo, Stefania, additional, Siragusa, Sergio, additional, Monteleone, Francesca, additional, Alessandro, Riccardo, additional, Fini, Milena, additional, and Giavaresi, Gianluca, additional

Published
2020
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38. A Four-Chemokine Signature Is Associated with a T-cell–Inflamed Phenotype in Primary and Metastatic Pancreatic Cancer

Author

Romero, Joan M., primary, Grünwald, Barbara, additional, Jang, Gun-Ho, additional, Bavi, Prashant P., additional, Jhaveri, Aaditeya, additional, Masoomian, Mehdi, additional, Fischer, Sandra E., additional, Zhang, Amy, additional, Denroche, Robert E., additional, Lungu, Ilinca M., additional, De Luca, Angela, additional, Bartlett, John M.S., additional, Xu, Jing, additional, Li, Niandong, additional, Dhaliwal, Sharon, additional, Liang, Sheng-Ben, additional, Chadwick, Dianne, additional, Vyas, Foram, additional, Bronsert, Peter, additional, Khokha, Rama, additional, McGaha, Tracy L., additional, Notta, Faiyaz, additional, Ohashi, Pamela S., additional, Done, Susan J., additional, O'Kane, Grainne M., additional, Wilson, Julie M., additional, Knox, Jennifer J., additional, Connor, Ashton, additional, Wang, Yifan, additional, Zogopoulos, George, additional, and Gallinger, Steven, additional

Published
2020
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40. Extracellular Vesicle microRNAs Contribute to the Osteogenic Inhibition of Mesenchymal Stem Cells in Multiple Myeloma

Author

Raimondo, Stefania, primary, Urzì, Ornella, additional, Conigliaro, Alice, additional, Lo Bosco, Giosuè, additional, Parisi, Sofia, additional, Carlisi, Melania, additional, Siragusa, Sergio, additional, Raimondi, Lavinia, additional, De Luca, Angela, additional, Giavaresi, Gianluca, additional, and Alessandro, Riccardo, additional

Published
2020
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44. Osteosarcoma cell-derived exosomes affect tumor microenvironment by specific packaging of microRNAs

Author

Raimondi, Lavinia, primary, De Luca, Angela, primary, Gallo, Alessia, primary, Costa, Viviana, primary, Russelli, Giovanna, primary, Cuscino, Nicola, primary, Manno, Mauro, primary, Raccosta, Samuele, primary, Carina, Valeria, primary, Bellavia, Daniele, primary, Conigliaro, Alice, primary, Alessandro, Riccardo, primary, Fini, Milena, primary, Conaldi, Pier Giulio, primary, and Giavaresi, Gianluca, primary

Published
2019
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46. Focused Ultrasound Effects on Osteosarcoma Cell Lines

Author

Agnese, Valentina, primary, Costa, Viviana, additional, Scoarughi, Gian Luca, additional, Corso, Cristiano, additional, Carina, Valeria, additional, De Luca, Angela, additional, Bellavia, Daniele, additional, Raimondi, Lavinia, additional, Pagani, Stefania, additional, Midiri, Massimo, additional, Stassi, Giorgio, additional, Alessandro, Riccardo, additional, Fini, Milena, additional, Barbato, Gaetano, additional, and Giavaresi, Gianluca, additional

Published
2019
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48. Adjuvant Biophysical Therapies in Osteosarcoma

Author

Carina, Valeria, primary, Costa, Viviana, additional, Sartori, Maria, additional, Bellavia, Daniele, additional, De Luca, Angela, additional, Raimondi, Lavinia, additional, Fini, Milena, additional, and Giavaresi, Gianluca, additional

Published
2019
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49. Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome

Author

Bozal-Basterra, Laura, Martín-Ruíz, Itziar, Pirone, Lucia, Liang, Yinwen, Sigurðsson, Jón Otti, Gonzalez-Santamarta, Maria, Giordano, Immacolata, Gabicagogeascoa, Estibaliz, de Luca, Angela, Rodríguez, Jose A, Wilkie, Andrew O M, Kohlhase, Jürgen, Eastwood, Deborah, Yale, Christopher, Olsen, Jesper V., Rauchman, Michael, Anderson, Kathryn V, Sutherland, James D, Barrio, Rosa, Bozal-Basterra, Laura, Martín-Ruíz, Itziar, Pirone, Lucia, Liang, Yinwen, Sigurðsson, Jón Otti, Gonzalez-Santamarta, Maria, Giordano, Immacolata, Gabicagogeascoa, Estibaliz, de Luca, Angela, Rodríguez, Jose A, Wilkie, Andrew O M, Kohlhase, Jürgen, Eastwood, Deborah, Yale, Christopher, Olsen, Jesper V., Rauchman, Michael, Anderson, Kathryn V, Sutherland, James D, and Barrio, Rosa

Abstract

Townes-Brocks syndrome (TBS) is characterized by a spectrum of malformations in the digits, ears, and kidneys. These anomalies overlap those seen in a growing number of ciliopathies, which are genetic syndromes linked to defects in the formation or function of the primary cilia. TBS is caused by mutations in the gene encoding the transcriptional repressor SALL1 and is associated with the presence of a truncated protein that localizes to the cytoplasm. Here, we provide evidence that SALL1 mutations might cause TBS by means beyond its transcriptional capacity. By using proximity proteomics, we show that truncated SALL1 interacts with factors related to cilia function, including the negative regulators of ciliogenesis CCP110 and CEP97. This most likely contributes to more frequent cilia formation in TBS-derived fibroblasts, as well as in a CRISPR/Cas9-generated model cell line and in TBS-modeled mouse embryonic fibroblasts, than in wild-type controls. Furthermore, TBS-like cells show changes in cilia length and disassembly rates in combination with aberrant SHH signaling transduction. These findings support the hypothesis that aberrations in primary cilia and SHH signaling are contributing factors in TBS phenotypes, representing a paradigm shift in understanding TBS etiology. These results open possibilities for the treatment of TBS.

Published
2018

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