48 results on '"De Cicco, Paola"'
Search Results
2. Flavonoids: Their putative neurologic roles, epigenetic changes, and gut microbiota alterations in Parkinson’s disease
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Açar, Yasemin, Ağagündüz, Duygu, De Cicco, Paola, and Capasso, Raffaele
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- 2023
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3. Chamomile essential oils exert anti-inflammatory effects involving human and murine macrophages: Evidence to support a therapeutic action
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De Cicco, Paola, Ercolano, Giuseppe, Sirignano, Carmina, Rubino, Valentina, Rigano, Daniela, Ianaro, Angela, and Formisano, Carmen
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- 2023
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4. Role of Arthrospira Platensis in Preventing and Treating High-Fat Diet-Induced Hypercholesterolemia in Adult Rats
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Cacciola, Nunzio Antonio, primary, De Cicco, Paola, additional, Milanović, Maja, additional, Milovanović, Ivan, additional, Mišan, Aleksandra, additional, Kojić, Danijela, additional, Simeunović, Jelica, additional, Blagojević, Dajana, additional, Popović, Tamara, additional, Arsić, Aleksandra, additional, Pilija, Vladimir, additional, Mandić, Anamarija, additional, Borrelli, Francesca, additional, and Milić, Nataša, additional
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- 2024
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5. Zosterabisphenone B, a new diarylheptanoid heterodimer from the seagrass Zostera marina, induces apoptosis cell death in colon cancer cells and reduces tumour growth in mice.
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Cacciola, Nunzio Antonio, De Cicco, Paola, Amico, Rebecca, Sepe, Fabrizia, Li, Yan, Grauso, Laura, Nanì, Maria Francesca, Scarpato, Silvia, Zidorn, Christian, Mangoni, Alfonso, and Borrelli, Francesca
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Colorectal cancer (CRC) is one of the most common malignant tumours worldwide. Diarylheptanoids, secondary metabolites isolated from Zostera marina, are of interest in natural products research due to their biological activities. Zosterabisphenone B (ZBP B) has recently been shown to inhibit the viability of CRC cells. The aim of this study was to investigate the therapeutic potential of ZBP B for targeting human CRC cells. Cell viability was determined using the MTT assay. Flow cytometry and Western blot analyses were used to assess apoptosis and autophagy. A CRC xenograft model was used to evaluate the in vivo effect of ZBP B. No cytotoxic effect on HCEC cells was observed in the in vitro experiments. ZBP B caused morphological changes in HCT116 colon cancer cells due to an increase in early and late apoptotic cell populations. Mechanistically, ZBP B led to an increase in cleaved caspase‐3, caspase‐8, caspase‐9, PARP and BID proteins and a decrease in Bcl‐2 and c‐Myc proteins. In the xenograft model of CRC, ZBP B led to a reduction in tumour growth. These results indicate that ZBP B exerts a selective cytotoxic effect on CRC cells by affecting apoptotic signalling pathways and reducing tumour growth in mice. Taken together, our results suggest that ZBP B could be a lead compound for the synthesis and development of CRC drugs. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Current Practices and Awareness of Anticancer Plants in the Traditional Healthcare System
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De Cicco, Paola, Panza, Elisabetta, Armogida, Chiara, Ercolano, Giuseppe, Cirino, Giuseppe, Ianaro, Angela, Akhtar, Mohd Sayeed, editor, and Swamy, Mallappa Kumara, editor
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- 2018
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7. Molecular Networking Revealed Unique UV-Absorbing Phospholipids: Favilipids from the Marine Sponge Clathria faviformis
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Scarpato, Silvia, primary, Teta, Roberta, additional, De Cicco, Paola, additional, Borrelli, Francesca, additional, Pawlik, Joseph R., additional, Costantino, Valeria, additional, and Mangoni, Alfonso, additional
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- 2023
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8. A Cytotoxic Heterodimeric Cyclic Diarylheptanoid with a Rearranged Benzene Ring from the Seagrass Zostera marina
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Grauso, Laura, primary, Li, Yan, additional, Scarpato, Silvia, additional, Cacciola, Nunzio Antonio, additional, De Cicco, Paola, additional, Zidorn, Christian, additional, and Mangoni, Alfonso, additional
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- 2022
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9. TRPM8 indicates poor prognosis in colorectal cancer patients and its pharmacological targeting reduces tumour growth in mice by inhibiting Wnt/β‐catenin signalling
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Pagano, Ester, primary, Romano, Barbara, additional, Cicia, Donatella, additional, Iannotti, Fabio A., additional, Venneri, Tommaso, additional, Lucariello, Giuseppe, additional, Nanì, Maria Francesca, additional, Cattaneo, Fabio, additional, De Cicco, Paola, additional, D'Armiento, Maria, additional, De Luca, Marcello, additional, Lionetti, Ruggiero, additional, Lama, Stefania, additional, Stiuso, Paola, additional, Zoppoli, Pietro, additional, Falco, Geppino, additional, Marchianò, Silvia, additional, Fiorucci, Stefano, additional, Capasso, Raffaele, additional, Di Marzo, Vincenzo, additional, Borrelli, Francesca, additional, and Izzo, Angelo A., additional
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- 2022
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10. Olive leaf extract inhibits metastatic melanoma spread through suppression of epithelial to mesenchymal transition
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De Cicco, Paola, primary, Ercolano, Giuseppe, additional, Tenore, Gian Carlo, additional, and Ianaro, Angela, additional
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- 2022
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11. TRPM8 indicates poor prognosis in colorectal cancer patients and its pharmacological targeting reduces tumour growth in mice by inhibiting Wnt/β‐catenin signalling.
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Pagano, Ester, Romano, Barbara, Cicia, Donatella, Iannotti, Fabio A., Venneri, Tommaso, Lucariello, Giuseppe, Nanì, Maria Francesca, Cattaneo, Fabio, De Cicco, Paola, D'Armiento, Maria, De Luca, Marcello, Lionetti, Ruggiero, Lama, Stefania, Stiuso, Paola, Zoppoli, Pietro, Falco, Geppino, Marchianò, Silvia, Fiorucci, Stefano, Capasso, Raffaele, and Di Marzo, Vincenzo
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ADENOMATOUS polyposis coli ,CANCER prognosis ,TRP channels ,COLON cancer ,PROGNOSIS ,NEUROPHARMACOLOGY - Abstract
Background and Purpose: Transient receptor potential melastatin type‐8 (TRPM8) is a cold‐sensitive cation channel protein belonging to the TRP superfamily of ion channels. Here, we reveal the molecular mechanism of TRPM8 and its clinical relevance in colorectal cancer (CRC). Experimental Approach: TRPM8 expression and its correlation with the survival rate of CRC patients was analysed. To identify the key pathways and genes related to TRPM8 high expression, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were conducted in CRC patients. TRPM8 functional role was assessed by using Trpm8−/− mice in models of sporadic and colitis‐associated colon cancer. TRPM8 pharmacological targeting by WS12 was evaluated in murine models of CRC. Key Results: TRPM8 is overexpressed in colon primary tumours and in CD326+ tumour cell fraction. TRPM8 high expression was related to lower survival rate of CRC patients, Wnt–Frizzled signalling hyperactivation and adenomatous polyposis coli down‐regulation. In sporadic and colitis‐associated models of colon cancer, either absence or pharmacological desensitization of TRPM8 reduced tumour development via inhibition of the oncogenic Wnt/β‐catenin signalling. TRPM8 pharmacological blockade reduced tumour growth in CRC xenograft mice by reducing the transcription of Wnt signalling regulators and the activation of β‐catenin and its target oncogenes such as C‐Myc and Cyclin D1. Conclusion and Implications: Human data provide valuable insights to propose TRPM8 as a prognostic marker with a negative predictive value for CRC patient survival. Animal experiments demonstrate TRPM8 involvement in colon cancer pathophysiology and its potential as a drug target for CRC. [ABSTRACT FROM AUTHOR]
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- 2023
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12. Role of the cystathionine γ lyase/hydrogen sulfide pathway in human melanoma progression
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Panza, Elisabetta, De Cicco, Paola, Armogida, Chiara, Scognamiglio, Giosuè, Gigantino, Vincenzo, Botti, Gerardo, Germano, Domenico, Napolitano, Maria, Papapetropoulos, Andreas, Bucci, Mariarosaria, Cirino, Giuseppe, and Ianaro, Angela
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- 2015
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13. Olive Leaf Extract, from Olea europaea L., Reduces Palmitate-Induced Inflammation via Regulation of Murine Macrophages Polarization
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De Cicco, Paola, primary, Maisto, Maria, additional, Tenore, Gian Carlo, additional, and Ianaro, Angela, additional
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- 2020
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14. Inhibitory effects of cynaropicrin on human melanoma progression by targeting MAPK, NF‐κB, and Nrf‐2 signaling pathways in vitro
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De Cicco, Paola, primary, Busà, Rosalia, additional, Ercolano, Giuseppe, additional, Formisano, Carmen, additional, Allegra, Mario, additional, Taglialatela‐Scafati, Orazio, additional, and Ianaro, Angela, additional
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- 2020
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15. The New Era of Cancer Immunotherapy: Targeting Myeloid-Derived Suppressor Cells to Overcome Immune Evasion
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De Cicco, Paola, primary, Ercolano, Giuseppe, additional, and Ianaro, Angela, additional
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- 2020
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16. Knockdown of PTGS2 by CRISPR/CAS9 System Designates a New Potential Gene Target for Melanoma Treatment
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Ercolano, Giuseppe, primary, De Cicco, Paola, additional, Rubino, Valentina, additional, Terrazzano, Giuseppe, additional, Ruggiero, Giuseppina, additional, Carriero, Roberta, additional, Kunderfranco, Paolo, additional, and Ianaro, Angela, additional
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- 2019
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17. Modulation of the functions of myeloid‐derived suppressor cells : a new strategy of hydrogen sulfide anti‐cancer effects
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De Cicco, Paola, primary, Ercolano, Giuseppe, additional, Rubino, Valentina, additional, Terrazzano, Giuseppe, additional, Ruggiero, Giuseppina, additional, Cirino, Giuseppe, additional, and Ianaro, Angela, additional
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- 2019
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18. Nutrition and Breast Cancer: A Literature Review on Prevention, Treatment and Recurrence
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De Cicco, Paola, primary, Catani, Maria Valeria, additional, Gasperi, Valeria, additional, Sibilano, Matteo, additional, Quaglietta, Maria, additional, and Savini, Isabella, additional
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- 2019
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19. Anti-metastatic Properties of Naproxen-HBTA in a Murine Model of Cutaneous Melanoma
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Ercolano, Giuseppe, primary, De Cicco, Paola, additional, Frecentese, Francesco, additional, Saccone, Irene, additional, Corvino, Angela, additional, Giordano, Flavia, additional, Magli, Elisa, additional, Fiorino, Ferdinando, additional, Severino, Beatrice, additional, Calderone, Vincenzo, additional, Citi, Valentina, additional, Cirino, Giuseppe, additional, and Ianaro, Angela, additional
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- 2019
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20. New Drugs from the Sea: Pro-Apoptotic Activity of Sponges and Algae Derived Compounds
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Ercolano, Giuseppe, primary, De Cicco, Paola, additional, and Ianaro, Angela, additional
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- 2019
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21. Indicaxanthin from Opuntia Ficus Indica (L. Mill) impairs melanoma cell proliferation, invasiveness, and tumor progression
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Allegra, Mario, primary, De Cicco, Paola, additional, Ercolano, Giuseppe, additional, Attanzio, Alessandro, additional, Busà, Rosalia, additional, Cirino, Giuseppe, additional, Tesoriere, Luisa, additional, Livrea, Maria A., additional, and Ianaro, Angela, additional
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- 2018
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22. MicroRNA-143-3p inhibits growth and invasiveness of melanoma cells by targeting cyclooxygenase-2 and inversely correlates with malignant melanoma progression
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Panza, Elisabetta, primary, Ercolano, Giuseppe, additional, De Cicco, Paola, additional, Armogida, Chiara, additional, Scognamiglio, Giosuè, additional, Botti, Gerardo, additional, Cirino, Giuseppe, additional, and Ianaro, Angela, additional
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- 2018
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23. Inhibitory effects of cynaropicrin on human melanoma progression by targeting MAPK, NF‐κB, and Nrf‐2 signaling pathways in vitro.
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De Cicco, Paola, Busà, Rosalia, Ercolano, Giuseppe, Formisano, Carmen, Allegra, Mario, Taglialatela‐Scafati, Orazio, and Ianaro, Angela
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Malignant melanoma is the deadliest skin cancer, due to its propensity to metastasize. MAPKs and NF‐κB pathways are constitutively activated in melanoma and promote cell proliferation, cell invasion, metastasis formation, and resistance to therapeutic regimens. Thus, they represent potential targets for melanoma prevention and treatment. Phytochemicals are gaining considerable attention for the management of melanoma because of their several cellular and molecular targets. A screening of a small library of sesquiterpenes lactones selected cynaropicrin, isolated from the aerial parts of Centaurea drabifolia subsp. detonsa, for its potential anticancer effect against melanoma cells. Treatment of human melanoma cells A375 with cynaropicrin resulted in inhibition of cell proliferation and induction of caspase‐3‐dependent apoptosis. Furthermore, cynaropicrin reduced several cellular malignant features such migration, invasion, and colonies formation through the inhibition of ERK1/2 and NF‐κB activity. Cynaropicrin was able to reduce intracellular reactive oxygen species generation, which are involved in all the stages of carcinogenesis. Indeed, cynaropicrin increased the expression of several antioxidant genes, such as glutamate–cysteine ligase and heme oxygenase‐1, by promoting the activation of the transcription factor Nrf‐2. In conclusion, our results individuate cynaropicrin as a potential adjuvant chemotherapeutic agent for melanoma by targeting several protumorigenic signaling pathways. [ABSTRACT FROM AUTHOR]
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- 2021
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24. Modulation of the functions of myeloid-derived suppressor cells : a new strategy of hydrogen sulfide anti-cancer effects.
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De Cicco, Paola, Ercolano, Giuseppe, Rubino, Valentina, Terrazzano, Giuseppe, Ruggiero, Giuseppina, Cirino, Giuseppe, and Ianaro, Angela
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SUPPRESSOR cells , *HYDROGEN sulfide , *T cells , *DENDRITIC cells , *IMMUNE response , *BIOLOGY - Abstract
Background and Purpose: Myeloid-derived suppressor cells (MDSCs) represent a major obstacle to cancer treatment, as they negatively regulate anti-tumour immunity through the suppression of tumour-specific T lymphocytes. Thus, the efficacy of immunotherapies may be improved by targeting MDSCs. In this study, we assessed the ability of hydrogen sulfide (H2 S), a gasotransmitter whose anti-cancer effects are well known, to inhibit the accumulation and immunosuppressive functions of MDSCs in melanoma.Experimental Approach: Effects of H2 S on the host immune response to cancer were evaluated using an in vivo syngeneic model of murine melanoma. B16F10-melanoma-bearing mice were treated with the H2 S donor, diallyl trisulfide (DATS) and analysed for content of MDSCs, dendritic cells (DCs) and T cells. Effects of H2 S on expression of immunosuppressive genes in MDSCs and on T cell proliferation were evaluated.Key Results: In melanoma-bearing mice, DATS inhibited tumour growth, and this effect was associated with a reduction in the frequency of MDSCs in the spleen, in the blood as well as in the tumour micro-environment. In addition, we found that CD8+ T cells and DCs were increased. Furthermore, DATS reduced the immuno-suppressive activity of MDSCs, restoring T cell proliferation.Conclusions and Implications: The H2 S donor compound, DATS, inhibited the expansion and the suppressive functions of MDSCs, suggesting a novel role for H2 S as a modulator of MDSCs in cancer. Therefore, H2 S donors may provide a novel approach for enhancing the efficacy of melanoma immunotherapy.Linked Articles: This article is part of a themed section on Hydrogen Sulfide in Biology & Medicine. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v177.4/issuetoc. [ABSTRACT FROM AUTHOR]- Published
- 2020
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25. Hydrogen Sulfide Reduces Myeloid-Derived Suppressor Cell-Mediated Inflammatory Response in a Model of Helicobacter hepaticus-Induced Colitis
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De Cicco, Paola, primary, Sanders, Theodore, additional, Cirino, Giuseppe, additional, Maloy, Kevin J., additional, and Ianaro, Angela, additional
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- 2018
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26. NMR-based phytochemical analysis of Vitis vinifera cv Falanghina leaves. Characterization of a previously undescribed biflavonoid with antiproliferative activity
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Tartaglione, Luciana, primary, Gambuti, Angelita, additional, De Cicco, Paola, additional, Ercolano, Giuseppe, additional, Ianaro, Angela, additional, Taglialatela-Scafati, Orazio, additional, Moio, Luigi, additional, and Forino, Martino, additional
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- 2018
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27. Melanoma and immunotherapy bridge 2015
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Nanda, Vashisht G. Y., Peng, Weiyi, Hwu, Patrick, Davies, Michael A., Ciliberto, Gennaro, Fattore, Luigi, Malpicci, Debora, Aurisicchio, Luigi, Ascierto, Paolo Antonio, Croce, Carlo M., Mancini, Rita, Spranger, Stefani, Gajewski, Thomas F., Wang, Yangyang, Ferrone, Soldano, Vanpouille-Box, Claire, Wennerberg, Erik, Pilones, Karsten A., Formenti, Silvia C., Demaria, Sandra, Tang, Haidong, Wang, Yang, Fu, Yang-Xin, Dummer, Reinhard, Puzanov, Igor, Tarhini, Ahmad, Chauvin, Joe-Marc, Pagliano, Ornella, Fourcade, Julien, Sun, Zhaojun, Wang, Hong, Sanders, Cindy, Kirkwood, John M., Chen, Tseng-hui Timothy, Maurer, Mark, Korman, Alan J., Zarour, Hassane M., Stroncek, David F., Huber, Veronica, Rivoltini, Licia, Thurin, Magdalena, Rau, Tilman, Lugli, Alessandro, Pagès, Franck, Camarero, Jorge, Sancho, Arantxa, Jommi, Claudio, de Coaña, Yago Pico, Wolodarski, Maria, Yoshimoto, Yuya, Gentilcore, Giusy, Poschke, Isabel, Masucci, Giuseppe V., Hansson, Johan, Kiessling, Rolf, Scognamiglio, Giosuè, Sabbatino, Francesco, Marino, Federica Zito, Anniciello, Anna Maria, Cantile, Monica, Cerrone, Margherita, Scala, Stefania, D’alterio, Crescenzo, Ianaro, Angela, Cirin, Giuseppe, Liguori, Giuseppina, Bott, Gerardo, Chapman, Paul B., Robert, Caroline, Larkin, James, Haanen, John B., Ribas, Antoni, Hogg, David, Hamid, Omid, Testori, Alessandro, Lorigan, Paul, Sosman, Jeffrey A., Flaherty, Keith T., Yue, Huibin, Coleman, Shelley, Caro, Ivor, Hauschild, Axel, McArthur, Grant A., Sznol, Mario, Callahan, Margaret K., Kluger, Harriet, Postow, Michael A., Gordan, RuthAnn, Segal, Neil H., Rizvi, Naiyer A., Lesokhin, Alexander, Atkins, Michael B., Burke, Matthew M., Ralabate, Amanda, Rivera, Angel, Kronenberg, Stephanie A., Agunwamba, Blessing, Ruisi, Mary, Horak, Christine, Jiang, Joel, Wolchok, Jedd, Ascierto, Paolo A., Liszkay, Gabriella, Maio, Michele, Mandalà, Mario, Demidov, Lev, Stoyakovskiy, Daniil, Thomas, Luc, de la Cruz-Merino, Luis, Atkinson, Victoria, Dutriaux, Caroline, Garbe, Claus, Wongchenko, Matthew, Chang, Ilsung, Koralek, Daniel O., Rooney, Isabelle, Yan, Yibing, Dréno, Brigitte, Sullivan, Ryan, Patel, Manish, Hodi, Stephen, Amaria, Rodabe, Boasberg, Peter, Wallin, Jeffrey, He, Xian, Cha, Edward, Richie, Nicole, Ballinger, Marcus, Smith, David C., Bauer, Todd M., Wasser, Jeffrey S., Luke, Jason J., Balmanoukian, Ani S., Kaufman, David R., Zhao, Yufan, Maleski, Janet, Leopold, Lance, Gangadhar, Tara C., Long, Georgina V., Michielin, Olivier, VanderWalde, Ari, Andtbacka, Robert H. I., Cebon, Jonathan, Fernandez, Eugenio, Malvehy, Josep, Olszanski, Anthony J., Gause, Christine, Chen, Lisa, Chou, Jeffrey, Stephen Hodi, F., Brady, Benjamin, Mortier, Laurent, Hassel, Jessica C., Rutkowski, Piotr, McNeil, Catriona, Kalinka-Warzocha, Ewa, Lebbé, Celeste, Ny, Lars, Chacon, Matias, Queirolo, Paola, Loquai, Carmen, Cheema, Parneet, Berrocal, Alfonso, Eizmendi, Karmele Mujika, Bar-Sela, Gil, Hardy, Helene, Weber, Jeffrey S., Grob, Jean-Jacques, Marquez-Rodas, Ivan, Schmidt, Henrik, Briscoe, Karen, Baurain, Jean-François, Wolchok, Jedd D., Pinto, Rosamaria, De Summa, Simona, Garrisi, Vito Michele, Strippoli, Sabino, Azzariti, Amalia, Guida, Gabriella, Guida, Michele, Tommasi, Stefania, Jacquelot, Nicolas, Enot, David, Flament, Caroline, Pitt, Jonathan M., Vimond, Nadège, Blattner, Carolin, Yamazaki, Takahiro, Roberti, Maria-Paula, Vetizou, Marie, Daillere, Romain, Poirier-Colame, Vichnou, la Semeraro, Michaë, Caignard, Anne, Slingluff, Craig L, Sallusto, Federica, Rusakiewicz, Sylvie, Weide, Benjamin, Marabelle, Aurélien, Kohrt, Holbrook, Dalle, Stéphane, Cavalcanti, Andréa, Kroemer, Guido, Di Giacomo, Anna Maria, Maio, Michaele, Wong, Phillip, Yuan, Jianda, Umansky, Viktor, Eggermont, Alexander, Zitvogel, Laurence, Anna, Passarelli, Marco, Tucci, Stefania, Stucci, Francesco, Mannavola, Mariaelena, Capone, Gabriele, Madonna, Antonio, Ascierto Paolo, Franco, Silvestris, Roberti, María Paula, Enot, David P., Semeraro, Michaela, Jégou, Sarah, Flores, Camila, Kwon, Byoung S., Anderson, Ana Carrizossa, Borg, Christophe, Aubin, François, Ayyoub, Maha, De Presbiteris, Anna Lisa, Cordaro, Fabiola Gilda, Camerlingo, Rosa, Fratangelo, Federica, Mozzillo, Nicola, Pirozzi, Giuseppe, Patriarca, Eduardo J., Caputo, Emilia, Motti, Maria Letizia, Falcon, Rosaria, Miceli, Roberta, Capone, Mariaelena, Madonna, Gabriele, Mallardo, Domenico, Carrier, Maria Vincenza, Panza, Elisabetta, De Cicco, Paola, Armogida, Chiara, Ercolano, Giuseppe, Botti, Gerardo, Cirino, Giuseppe, Sandru, Angela, Blank, Miri, Balatoni, Timea, Olasz, Judit, Farkas, Emil, Szollar, Andras, Savolt, Akos, Godeny, Maria, Csuka, Orsolya, Horvath, Szabolcs, Eles, Klara, Shoenfeld, Yehuda, Kasler, Miklos, Costantini, Susan, Capone, Francesca, Moradi, Farnaz, Berglund, Pontus, Leandersson, Karin, Linnskog, Rickard, Andersson, Tommy, Prasad, Chandra Prakash, Nigro, Cristiana Lo, Lattanzio, Laura, Wang, Hexiao, Proby, Charlotte, Syed, Nelofer, Occelli, Marcella, Cauchi, Carolina, Merlano, Marco, Harwood, Catherine, Thompson, Alastair, Crook, Tim, Bifulco, Katia, Ingangi, Vincenzo, Minopoli, Michele, Ragone, Concetta, Pessi, Antonello, Mannavola, Francesco, D’Oronzo, Stella, Felici, Claudia, Tucci, Marco, Doronzo, Antonio, Silvestris, Franco, Ferretta, Anna, Guida, Stefania, Maida, Imma, Cocco, Tiziana, Passarelli, Anna, Quaresmini, Davide, Franzese, Ornella, Palermo, Belinda, Di Donna, Cosmo, Sperduti, Isabella, Foddai, MariaLaura, Stabile, Helena, Gismondi, Angela, Santoni, Angela, Nisticò, Paola, Sponghini, Andrea P., Platini, Francesca, Marra, Elena, Rondonotti, David, Alabiso, Oscar, Fierro, Maria T., Savoia, Paola, Stratica, Florian, Quaglino, Pietro, Di Monta, Gianluca, Corrado, Caracò, Di Marzo, Massimiliano, Ugo, Marone, Di Cecilia, Maria Luisa, Nicola, Mozzillo, Fusciello, Celeste, Marra, Antonio, Guarrasi, Rosario, Baldi, Carlo, Russo, Rosa, Di Giulio, Giovanni, Faiola, Vincenzo, Zeppa, Pio, Pepe, Stefano, Gambale, Elisabetta, Carella, Consiglia, Di Paolo, Alessandra, De Tursi, Michele, Marra, Laura, De Murtas, Fara, Sorrentino, Valeria, Voinea, Silviu, Panaitescu, Eugenia, Bolovan, Madalina, Stanciu, Adina, Cinca, Sabin, Botti, Chiara, Aquino, Gabriella, Anniciello, Annamaria, Fortes, Cristina, Mastroeni, Simona, Caggiati, Alessio, Passarelli, Francesca, Zappalà, Alba, Capuano, Maria, Bono, Riccardo, Nudo, Maurizio, Marino, Claudia, Michelozzi, Paola, De Biasio, Valeria, Battarra, Vincenzo C., Formenti, Silvia, Ascierto, Maria Libera, McMiller, Tracee L., Berger, Alan E., Danilova, Ludmila, Anders, Robert A., Netto, George J., Xu, Haiying, Pritchard, Theresa S., Fan, Jinshui, Cheadle, Chris, Cope, Leslie, Drake, Charles G., Pardoll, Drew M., Taube, Janis M., Topalian, Suzanne L., Gnjatic, Sacha, Nataraj, Sarah, Imai, Naoko, Rahman, Adeeb, Jungbluth, Achim A., Pan, Linda, Venhaus, Ralph, Park, Andrew, Lehmann, Frédéric F., Lendvai, Nikoletta, Cohen, Adam D., Cho, Hearn J., Daniel, Speiser, and Hirsh, Vera
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Medicine(all) ,Biochemistry, Genetics and Molecular Biology(all) ,Meeting Abstracts - Abstract
Table of contents MELANOMA BRIDGE 2015 KEYNOTE SPEAKER PRESENTATIONS Molecular and immuno-advances K1 Immunologic and metabolic consequences of PI3K/AKT/mTOR activation in melanoma Vashisht G. Y. Nanda, Weiyi Peng, Patrick Hwu, Michael A. Davies K2 Non-mutational adaptive changes in melanoma cells exposed to BRAF and MEK inhibitors help the establishment of drug resistance Gennaro Ciliberto, Luigi Fattore, Debora Malpicci, Luigi Aurisicchio, Paolo Antonio Ascierto, Carlo M. Croce, Rita Mancini K3 Tumor-intrinsic beta-catenin signaling mediates tumor-immune avoidance Stefani Spranger, Thomas F. Gajewski K4 Intracellular tumor antigens as a source of targets of antibody-based immunotherapy of melanoma Yangyang Wang, Soldano Ferrone Combination therapies K5 Harnessing radiotherapy to improve responses to immunotherapy in cancer Claire Vanpouille-Box, Erik Wennerberg, Karsten A. Pilones, Silvia C. Formenti, Sandra Demaria K6 Creating a T cell-inflamed tumor microenvironment overcomes resistance to checkpoint blockade Haidong Tang, Yang Wang, Yang-Xin Fu K7 Biomarkers for treatment decisions? Reinhard Dummer K8 Combining oncolytic therapies in the era of checkpoint inhibitors Igor Puzanov K9 Immune checkpoint blockade for melanoma: should we combine or sequence ipilimumab and PD-1 antibody therapy? Michael A. Postow News in immunotherapy K10 An update on adjuvant and neoadjuvant therapy for melanom Ahmad Tarhini K11 Targeting multiple inhibitory receptors in melanoma Joe-Marc Chauvin, Ornella Pagliano, Julien Fourcade, Zhaojun Sun, Hong Wang, Cindy Sanders, John M. Kirkwood, Tseng-hui Timothy Chen, Mark Maurer, Alan J. Korman, Hassane M. Zarour K12 Improving adoptive immune therapy using genetically engineered T cells David F. Stroncek Tumor microenvironment and biomarkers K13 Myeloid cells and tumor exosomes: a crosstalk for assessing immunosuppression? Veronica Huber, Licia Rivoltini K14 Update on the SITC biomarker taskforce: progress and challenges Magdalena Thurin World-wide immunoscore task force: an update K15 The immunoscore in colorectal cancer highlights the importance of digital scoring systems in surgical pathology Tilman Rau, Alessandro Lugli K16 The immunoscore: toward an integrated immunomonitoring from the diagnosis to the follow up of cancer’s patients Franck Pagès Economic sustainability of melanoma treatments: regulatory, health technology assessment and market access issues K17 Nivolumab, the regulatory experience in immunotherapy Jorge Camarero, Arantxa Sancho K18 Evidence to optimize access for immunotherapies Claudio Jommi ORAL PRESENTATIONS Molecular and immuno-advances O1 Ipilimumab treatment results in CD4 T cell activation that is concomitant with a reduction in Tregs and MDSCs Yago Pico de Coaña, Maria Wolodarski, Yuya Yoshimoto, Giusy Gentilcore, Isabel Poschke, Giuseppe V. Masucci, Johan Hansson, Rolf Kiessling O2 Evaluation of prognostic and therapeutic potential of COX-2 and PD-L1 in primary and metastatic melanoma Giosuè Scognamiglio, Francesco Sabbatino, Federica Zito Marino, Anna Maria Anniciello, Monica Cantile, Margherita Cerrone, Stefania Scala, Crescenzo D’alterio, Angela Ianaro, Giuseppe Cirino, Paolo Antonio Ascierto, Giuseppina Liguori, Gerardo Botti O3 Vemurafenib in patients with BRAFV600 mutation–positive metastatic melanoma: final overall survival results of the BRIM-3 study Paul B. Chapman, Caroline Robert, James Larkin, John B. Haanen, Antoni Ribas, David Hogg, Omid Hamid, Paolo Antonio Ascierto, Alessandro Testori, Paul Lorigan, Reinhard Dummer, Jeffrey A. Sosman, Keith T. Flaherty, Huibin Yue, Shelley Coleman, Ivor Caro, Axel Hauschild, Grant A. McArthur O4 Updated survival, response and safety data in a phase 1 dose-finding study (CA209-004) of concurrent nivolumab (NIVO) and ipilimumab (IPI) in advanced melanoma Mario Sznol, Margaret K. Callahan, Harriet Kluger, Michael A. Postow, RuthAnn Gordan, Neil H. Segal, Naiyer A. Rizvi, Alexander Lesokhin, Michael B. Atkins, John M. Kirkwood, Matthew M. Burke, Amanda Ralabate, Angel Rivera, Stephanie A. Kronenberg, Blessing Agunwamba, Mary Ruisi, Christine Horak, Joel Jiang, Jedd Wolchok Combination therapies O5 Efficacy and correlative biomarker analysis of the coBRIM study comparing cobimetinib (COBI) + vemurafenib (VEM) vs placebo (PBO) + VEM in advanced BRAF-mutated melanoma patients (pts) Paolo A. Ascierto, Grant A. McArthur, James Larkin, Gabriella Liszkay, Michele Maio, Mario Mandalà, Lev Demidov, Daniil Stoyakovskiy, Luc Thomas, Luis de la Cruz-Merino, Victoria Atkinson, Caroline Dutriaux, Claus Garbe, Matthew Wongchenko, Ilsung Chang, Daniel O. Koralek, Isabelle Rooney, Yibing Yan, Antoni Ribas, Brigitte Dréno O6 Preliminary clinical safety, tolerability and activity results from a Phase Ib study of atezolizumab (anti-PDL1) combined with vemurafenib in BRAFV600-mutant metastatic melanoma Ryan Sullivan, Omid Hamid, Manish Patel, Stephen Hodi, Rodabe Amaria, Peter Boasberg, Jeffrey Wallin, Xian He, Edward Cha, Nicole Richie, Marcus Ballinger, Patrick Hwu O7 Preliminary safety and efficacy data from a phase 1/2 study of epacadostat (INCB024360) in combination with pembrolizumab in patients with advanced/metastatic melanoma Thomas F. Gajewski, Omid Hamid, David C. Smith, Todd M. Bauer, Jeffrey S. Wasser, Jason J. Luke, Ani S. Balmanoukian, David R. Kaufman, Yufan Zhao, Janet Maleski, Lance Leopold, Tara C. Gangadhar O8 Primary analysis of MASTERKEY-265 phase 1b study of talimogene laherparepvec (T-VEC) and pembrolizumab (pembro) for unresectable stage IIIB-IV melanoma Reinhard Dummer, Georgina V. Long, Antoni Ribas, Igor Puzanov, Olivier Michielin, Ari VanderWalde, Robert H.I. Andtbacka, Jonathan Cebon, Eugenio Fernandez, Josep Malvehy, Anthony J. Olszanski, Thomas F. Gajewski, John M. Kirkwood, Christine Gause, Lisa Chen, David R. Kaufman, Jeffrey Chou, F. Stephen Hodi News in immunotherapy O9 Two-year survival and safety update in patients (pts) with treatment-naïve advanced melanoma (MEL) receiving nivolumab (NIVO) or dacarbazine (DTIC) in CheckMate 066 Victoria Atkinson, Paolo A. Ascierto, Georgina V. Long, Benjamin Brady, Caroline Dutriaux, Michele Maio, Laurent Mortier, Jessica C. Hassel, Piotr Rutkowski, Catriona McNeil, Ewa Kalinka-Warzocha, Celeste Lebbé, Lars Ny, Matias Chacon, Paola Queirolo, Carmen Loquai, Parneet Cheema, Alfonso Berrocal, Karmele Mujika Eizmendi, Luis De La Cruz-Merino, Gil Bar-Sela, Christine Horak, Joel Jiang, Helene Hardy, Caroline Robert O10 Efficacy and safety of nivolumab (NIVO) in patients (pts) with advanced melanoma (MEL) who were treated beyond progression in CheckMate 066/067 Georgina V. Long, Jeffrey S. Weber, James Larkin, Victoria Atkinson, Jean-Jacques Grob, Reinhard Dummer, Caroline Robert, Ivan Marquez-Rodas, Catriona McNeil, Henrik Schmidt, Karen Briscoe, Jean-François Baurain, F. Stephen Hodi, Jedd D. Wolchok Tumor microenvironment and biomarkers O11 New biomarkers for response/resistance to BRAF inhibitor therapy in metastatic melanoma Rosamaria Pinto, Simona De Summa, Vito Michele Garrisi, Sabino Strippoli, Amalia Azzariti, Gabriella Guida, Michele Guida, Stefania Tommasi O12 Chemokine receptor patterns in lymphocytes mirror metastatic spreading in melanoma and response to ipilimumab Nicolas Jacquelot, David Enot, Caroline Flament, Jonathan M. Pitt, Nadège Vimond, Carolin Blattner, Takahiro Yamazaki, Maria-Paula Roberti, Marie Vetizou, Romain Daillere, Vichnou Poirier-Colame, Michaëla Semeraro, Anne Caignard, Craig L Slingluff Jr, Federica Sallusto, Sylvie Rusakiewicz, Benjamin Weide, Aurélien Marabelle, Holbrook Kohrt, Stéphane Dalle, Andréa Cavalcanti, Guido Kroemer, Anna Maria Di Giacomo, Michaele Maio, Phillip Wong, Jianda Yuan, Jedd Wolchok, Viktor Umansky, Alexander Eggermont, Laurence Zitvogel O13 Serum levels of PD1- and CD28-positive exosomes before Ipilimumab correlate with therapeutic response in metastatic melanoma patients Passarelli Anna, Tucci Marco, Stucci Stefania, Mannavola Francesco, Capone Mariaelena, Madonna Gabriele, Ascierto Paolo Antonio, Silvestris Franco O14 Immunological prognostic factors in stage III melanomas María Paula Roberti, Nicolas Jacquelot, David P Enot, Sylvie Rusakiewicz, Michaela Semeraro, Sarah Jégou, Camila Flores, Lieping Chen, Byoung S. Kwon, Ana Carrizossa Anderson, Caroline Robert, Christophe Borg, Benjamin Weide, François Aubin, Stéphane Dalle, Michele Maio, Jedd D. Wolchok, Holbrook Kohrt, Maha Ayyoub, Guido Kroemer, Aurélien Marabelle, Andréa Cavalcanti, Alexander Eggermont, Laurence Zitvogel POSTER PRESENTATIONS Molecular and immuno-advances P1 Human melanoma cells resistant to B-RAF and MEK inhibition exhibit mesenchymal-like features Anna Lisa De Presbiteris, Fabiola Gilda Cordaro, Rosa Camerlingo, Federica Fratangelo, Nicola Mozzillo, Giuseppe Pirozzi, Eduardo J. Patriarca, Paolo A. Ascierto, Emilia Caputo P2 Anti-proliferative and pro-apoptotic effect of ABT888 on melanoma cell lines and its potential role in the treatment of melanoma resistant to B-RAF inhibitors Federica Fratangelo, Rosa Camerlingo, Emilia Caputo, Maria Letizia Motti, Rosaria Falcone, Roberta Miceli, Mariaelena Capone, Gabriele Madonna, Domenico Mallardo, Maria Vincenza Carriero, Giuseppe Pirozzi and Paolo Antonio Ascierto P3 Involvement of the L-cysteine/CSE/H2S pathway in human melanoma progression Elisabetta Panza, Paola De Cicco, Chiara Armogida, Giuseppe Ercolano, Rosa Camerlingo, Giuseppe Pirozzi, Giosuè Scognamiglio, Gerardo Botti, Giuseppe Cirino, Angela Ianaro P4 Cancer stem cell antigen revealing pattern of antibody variable region genes were defined by immunoglobulin repertoire analysis in patients with malignant melanoma Beatrix Kotlan, Gabriella Liszkay, Miri Blank, Timea Balatoni, Judit Olasz, Emil Farkas, Andras Szollar, Akos Savolt, Maria Godeny, Orsolya Csuka, Szabolcs Horvath, Klara Eles, Yehuda Shoenfeld and Miklos Kasler P5 Upregulation of Neuregulin-1 expression is a hallmark of adaptive response to BRAF/MEK inhibitors in melanoma Debora Malpicci, Luigi Fattore, Susan Costantini, Francesca Capone, Paolo Antonio Ascierto, Rita Mancini, Gennaro Ciliberto P6 HuR positively regulates migration of HTB63 melanoma cells Farnaz Moradi, Pontus Berglund, Karin Leandersson, Rickard Linnskog, Tommy Andersson, Chandra Prakash Prasad P7 Prolyl 4- (C-P4H) hydroxylases have opposing effects in malignant melanoma: implication in prognosis and therapy Cristiana Lo Nigro, Laura Lattanzio, Hexiao Wang, Charlotte Proby, Nelofer Syed, Marcella Occelli, Carolina Cauchi, Marco Merlano, Catherine Harwood, Alastair Thompson, Tim Crook P8 Urokinase receptor antagonists: novel agents for the treatment of melanoma Maria Letizia Motti, Katia Bifulco, Vincenzo Ingangi, Michele Minopoli, Concetta Ragone, Federica Fratangelo, Antonello Pessi, Gennaro Ciliberto, Paolo Antonio Ascierto, Maria Vincenza Carriero P9 Exosomes released by melanoma cell lines enhance chemotaxis of primary tumor cells Francesco Mannavola, Stella D’Oronzo, Claudia Felici, Marco Tucci, Antonio Doronzo, Franco Silvestris P10 New insights in mitochondrial metabolic reprogramming in melanoma Anna Ferretta, Gabriella Guida, Stefania Guida, Imma Maida, Tiziana Cocco, Sabino Strippoli, Stefania Tommasi, Amalia Azzariti, Michele Guida P11 Lenalidomide restrains the proliferation in melanoma cells through a negative regulation of their cell cycle Stella D’Oronzo, Anna Passarelli, Claudia Felici, Marco Tucci, Davide Quaresmini, Franco Silvestris Combination therapies P12 Chemoimmunotherapy elicits polyfunctional anti-tumor CD8 + T cells depending on the activation of an AKT pathway sustained by ICOS Ornella Franzese, Belinda Palermo, Cosmo Di Donna, Isabella Sperduti, MariaLaura Foddai, Helena Stabile, Angela Gismondi, Angela Santoni, Paola Nisticò P13 Favourable toxicity profile of combined BRAF and MEK inhibitors in metastatic melanoma patients Andrea P. Sponghini, Francesca Platini, Elena Marra, David Rondonotti, Oscar Alabiso, Maria T. Fierro, Paola Savoia, Florian Stratica, Pietro Quaglino P14 Electrothermal bipolar vessel sealing system dissection reduces seroma output or time to drain removal following axillary and ilio-inguinal node dissection in melanoma patients: a pilot study Di Monta Gianluca, Caracò Corrado, Di Marzo Massimiliano, Marone Ugo, Di Cecilia Maria Luisa, Mozzillo Nicola News in immunotherapy P15 Clinical and immunological response to ipilimumab in a metastatic melanoma patient with HIV infection Francesco Sabbatino, Celeste Fusciello1, Antonio Marra, Rosario Guarrasi, Carlo Baldi, Rosa Russo, Di Giulio Giovanni, Vincenzo Faiola, Pio Zeppa, Stefano Pepe P16 Immunotherapy and hypophysitis: a case report Elisabetta Gambale, Consiglia Carella, Alessandra Di Paolo, Michele De Tursi Tumor microenvironment and biomarkers P17 New immuno- histochemical markers for the differential diagnosis of atypical melanocytic lesions with uncertain malignant potential Laura Marra, Giosuè Scognamiglio, Monica Cantile, Margherita Cerrone, Fara De Murtas, Valeria Sorrentino, Anna Maria Anniciello, Gerardo Botti P18 Utility of simultaneous measurement of three serum tumor markers in melanoma patients Angela Sandru, Silviu Voinea, Eugenia Panaitescu, Madalina Bolovan, Adina Stanciu, Sabin Cinca P19 The significance of various cut-off levels of melanoma inhibitory activity in evaluation of cutaneous melanoma patients Angela Sandru, Silviu Voinea, Eugenia Panaitescu, Madalina Bolovan, Adina Stanciu, Sabin Cinca P20 The long noncoding RNA HOTAIR is associated to metastatic progression of melanoma and it can be identified in the blood of patients with advanced disease Chiara Botti, Giosuè Scognamiglio, Laura Marra, Gabriella Aquino, Rosaria Falcone, Annamaria Anniciello, Paolo Antonio Ascierto, Gerardo Botti, Monica Cantile Other P21 The effect of Sentinel Lymph Node Biopsy in melanoma mortality: timing of dissection Cristina Fortes, Simona Mastroeni, Alessio Caggiati, Francesca Passarelli, Alba Zappalà, Maria Capuano, Riccardo Bono, Maurizio Nudo, Claudia Marino, Paola Michelozzi P22 Epidemiological survey on related psychopathology in melanoma Valeria De Biasio, Vincenzo C. Battarra IMMUNOTHERAPY BRIDGE KEYNOTE SPEAKER PRESENTATIONS Immunotherapy beyond melanoma K19 Predictor of response to radiation and immunotherapy Silvia Formenti K20 Response and resistance to PD-1 pathway blockade: clues from the tumor microenvironment Maria Libera Ascierto, Tracee L. McMiller, Alan E. Berger, Ludmila Danilova, Robert A. Anders, George J. Netto, Haiying Xu, Theresa S. Pritchard, Jinshui Fan, Chris Cheadle, Leslie Cope, Charles G. Drake, Drew M. Pardoll, Janis M. Taube and Suzanne L. Topalian K21 Combination immunotherapy with autologous stem cell transplantation, protein immunization, and PBMC reinfusion in myeloma patients Sacha Gnjatic, Sarah Nataraj, Naoko Imai, Adeeb Rahman, Achim A. Jungbluth, Linda Pan, Ralph Venhaus, Andrew Park, Frédéric F. Lehmann, Nikoletta Lendvai, Adam D. Cohen, and Hearn J. Cho K22 Anti-cancer immunity despite T cell “exhaustion” Speiser Daniel Immunotherapy in oncology (I-O): data from clinical trial K23 The Checkpoint Inhibitors for the Treatment of Metastatic Non-small Cell Lung Cancer (NSCLC) Vera Hirsh
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- 2016
28. The Hydrogen Sulfide Releasing Molecule Acetyl Deacylasadisulfide Inhibits Metastatic Melanoma
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De Cicco, Paola, primary, Panza, Elisabetta, additional, Armogida, Chiara, additional, Ercolano, Giuseppe, additional, Taglialatela-Scafati, Orazio, additional, Shokoohinia, Yalda, additional, Camerlingo, Rosa, additional, Pirozzi, Giuseppe, additional, Calderone, Vincenzo, additional, Cirino, Giuseppe, additional, and Ianaro, Angela, additional
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- 2017
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29. ATB-346, a novel hydrogen sulfide-releasing anti-inflammatory drug, induces apoptosis of human melanoma cells and inhibits melanoma development in vivo
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De Cicco, Paola, primary, Panza, Elisabetta, additional, Ercolano, Giuseppe, additional, Armogida, Chiara, additional, Sessa, Giuseppe, additional, Pirozzi, Giuseppe, additional, Cirino, Giuseppe, additional, Wallace, John L., additional, and Ianaro, Angela, additional
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- 2016
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30. Involvement of the L-cysteine/hydrogen sulphide pathway in human melanoma
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DE CICCO, PAOLA, PANZA, ELISABETTA, BUCCI, MARIAROSARIA, CIRINO, GIUSEPPE, IANARO, ANGELA, Ianaro, Angela, Panza, Elisabetta, DE CICCO, Paola, Bucci, Mariarosaria, and Cirino, Giuseppe
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- 2013
31. TRANSCRIPTION FACTORS AS TARGETS OF NEW MOLECULES WITH POTENTIAL ANTI-CANCER ACTIVITY
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De Cicco, Paola
- Abstract
Melanoma is the deadliest form of skin cancer and it is rising in incidence. Although in the past 3 years have been made notable advances in the understanding the molecular pathogenesis of melanoma and its treatment, melanoma therapy is still a challenge. Therefore, it is critical to identify other important potential targets in melanoma development and progression that are amenable to pharmacological inhibition. In the last few years, numerous physiological and pathophysiological effects have been proposed for the gasotransmitter hydrogen sulphide, even its role in cancer molecular mechanisms is still unclear. Recently, it has been shown that CSE enzyme is expressed in melanoma cell lines and that CBS enzyme plays a key role in colon and ovarian cancer. Starting from these evidence, the aim of my PhD project was to investigate the role of the metabolic H2S pathway in human melanoma. Our study have demonstrate that H2S inhibited melanoma cell proliferation inducing apoptosis and cell cycle arrest in G0/G1-phase.The main pro-apoptotic mechanisms involved were suppression of nuclear factor-kB activity and inhibition of the AKT and ERK pathways.The anti-proliferative role of H2S was also confirmed by the finding that the overexpression of CSE induced spontaneous apoptosis of human melanoma cells. A proof of concept was obtained in vivo using both a murine model of cutaneus melanoma and a murine model of metastatic melanoma. In fact, either L-cysteine, the CSE substrate, or DATS inhibited tumor growth and the development of lung metastases in mice. Finally, the immunohistochemistry analysis performed on human melanoma samples demonstrated that CSE expression was highest in primary tumors, decreased in the metastatic lesions and was almost silent in non-lymph node metastase whereas CBS and 3-MST do not appear to play an important role in human melanoma. These data support our hypothesis that CSE derived hydrogen sulfide plays a major role in melanoma.In conclusion, we have determined that the L-cysteine/CSE/H2S pathway is involved in melanoma progression.
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- 2015
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32. Hydrogen sulfide as an endogenous 'controller' of human melanoma progression
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Panza, Elisabetta De Cicco, Paola Armogida, Chiara Ercolano, Giuseppe Gigantino, Vincenzo Botti, Gerardo Napolitano, Maria Papapetropoulos, Andreas Iacono, Valentina Mattera and Bucci, Mariarosaria Chino, Giuseppe Ianaro, Angela
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- 2015
33. Differential expression of cyclooxygenase-2 in metastatic melanoma affects progression free survival
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Panza, Elisabetta, primary, De Cicco, Paola, additional, Ercolano, Giuseppe, additional, Armogida, Chiara, additional, Scognamiglio, Giosuè, additional, Anniciello, Anna Maria, additional, Botti, Gerardo, additional, Cirino, Giuseppe, additional, and Ianaro, Angela, additional
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- 2016
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34. Hydrogen sulfide as an endogenous “controller” of human melanoma progression
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Panza, Elisabetta, primary, De Cicco, Paola, additional, Armogida, Chiara, additional, Ercolano, Giuseppe, additional, Gigantino, Vincenzo, additional, Botti, Gerardo, additional, Napolitano, Maria, additional, Papapetropoulos, Andreas, additional, Iacono, Valentina Mattera, additional, Bucci, Mariarosaria, additional, Cirino, Giuseppe, additional, and Ianaro, Angela, additional
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- 2015
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35. Hydrogen sulfide donors: New potential agents in melanoma treatment
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Panza, Elisabetta, primary, Armogida, Chiara, additional, De Cicco, Paola, additional, Ercolano, Giuseppe, additional, Scognamiglio, Giosuè, additional, Scafati, Orazio Taglialatela, additional, Cirino, Giuseppe, additional, and Ianaro, Angela, additional
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- 2015
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36. Role of the cystathionineγlyase/hydrogen sulfide pathway in human melanoma progression
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Panza, Elisabetta, primary, De Cicco, Paola, additional, Armogida, Chiara, additional, Scognamiglio, Giosuè, additional, Gigantino, Vincenzo, additional, Botti, Gerardo, additional, Germano, Domenico, additional, Napolitano, Maria, additional, Papapetropoulos, Andreas, additional, Bucci, Mariarosaria, additional, Cirino, Giuseppe, additional, and Ianaro, Angela, additional
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- 2014
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37. P37 Pro-apoptotic effect of hydrogen sulphide in human melanoma cell lines: A signal transduction analysis
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Ianaro, Angela, primary, Panza, Elisabetta, additional, Napolitano, Maria, additional, De Cicco, Paola, additional, Bucci, Mariarosaria, additional, Vellecco, Valentina, additional, Brancaleone, Vincenzo, additional, and Cirino, Giuseppe, additional
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- 2012
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38. Butyrate Attenuates Lipopolysaccharide-Induced Inflammation in Intestinal Cells and Crohn's Mucosa through Modulation of Antioxidant Defense Machinery
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Russo, Ilaria, primary, Luciani, Alessandro, additional, De Cicco, Paola, additional, Troncone, Edoardo, additional, and Ciacci, Carolina, additional
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- 2012
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39. PP69 - Hydrogen sulfide as an endogenous “controller” of human melanoma progression.
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Panza, Elisabetta, De Cicco, Paola, Armogida, Chiara, Ercolano, Giuseppe, Gigantino, Vincenzo, Botti, Gerardo, Napolitano, Maria, Papapetropoulos, Andreas, Iacono, Valentina Mattera, Bucci, Mariarosaria, Cirino, Giuseppe, and Ianaro, Angela
- Subjects
- *
HYDROGEN sulfide , *ENDOGENOUS hydrogen sulfide , *MELANOMA , *CANCER invasiveness , *ONCOLOGY , *PREVENTION - Published
- 2015
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40. A Cytotoxic Heterodimeric Cyclic Diarylheptanoid with a Rearranged Benzene Ring from the Seagrass Zostera marina
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Laura Grauso, Yan Li, Silvia Scarpato, Nunzio Antonio Cacciola, Paola De Cicco, Christian Zidorn, Alfonso Mangoni, Grauso, Laura, Li, Yan, Scarpato, Silvia, Cacciola, NUNZIO ANTONIO, DE CICCO, Paola, Zidorn, Christian, and Mangoni, Alfonso
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Pharmacology ,Complementary and alternative medicine ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,Analytical Chemistry - Abstract
The widespread seagrass Zostera marina contains a new diarylheptanoid heterodimer, zosterabisphenone C (1), featuring an unprecedented rearrangement of one of its benzene rings to a cyclopentenecarbonyl unit. The planar structure and absolute configuration of zosterabisphenone C were elucidated by a combination of spectroscopic (MS, ECD, and low-temperature NMR) and computational (DFT-NMR and DFT-ECD) evidence. Consistent with the previously isolated zosterabisphenones, compound 1 was selectively cytotoxic against HCT 116 adenocarcinoma colon cancer cells, reducing their viability by 73% at 10 μM (IC50 of 7.6 ± 1.1 μM). The biosynthetic origin of zosterabisphenone C (1) from an oxidative rearrangement of zosterabisphenone A (4) is proposed.
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- 2022
41. Chamomile essential oils exert anti-inflammatory effects involving human and murine macrophages: Evidence to support a therapeutic action
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Paola De Cicco, Giuseppe Ercolano, Carmina Sirignano, Valentina Rubino, Daniela Rigano, Angela Ianaro, Carmen Formisano, DE CICCO, Paola, Ercolano, Giuseppe, Sirignano, Carmina, Rubino, Valentina, Rigano, Daniela, Ianaro, Angela, and Formisano, Carmen
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Inflammation ,Pharmacology ,Matricaria ,Macrophage ,Drug Discovery ,Chamomile ,Phytotherapy - Abstract
Ethnopharmacological relevance: Chamomile (M. chamomilla L.) is an herbaceous plant from family Astereaceae, that has a long history of use in traditional medicine. It has been used as herbal remedies for thousands of years to treat several diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders. Chronic inflammation is involved in the pathogenesis of most infectious and non-infectious diseases and macrophages are considered the major cellular players that drive disease initiation and maintenance. Aim of the study: The aim of this study was to evaluate the variation in the chemical profile of the essential oil of M. chamomilla plants collected in three experimental field sites in the Molise region. Additionally, we evaluated the pharmacological mechanism behind the anti-inflammatory effect of M. chamomilla essential oils. Material and methods: Three essential oils (called GC1, GC2 and GC3) were extracted from aerial parts of M. chamomilla by hydrodistillation and chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). The essential oils were tested for their ability to modulate pro-inflammatory murine macrophages and human peripheral blood mononuclear cells (PBMCs) functions. Results: The chemical analysis of the samples revealed the presence of a high content of the oxygenated sesquiterpenes that represented more than the half of the entire oils. GC1, GC2 and GC3 essential oils significantly attenuated LPS/IFN-γ-induced inflammation by reducing M1 polarization. In details, they showed significant anti-inflammatory property by inhibiting NO, TNF-α and IL-6 production. These effects were correlated to a suppression of LPS-mediated p65 activation, the critical transactivation subunit for NF-κB transcription factor. Oxidative stress may trigger macrophages activation and elicit strong immune responses. Our study demonstrated that GC1, GC2 and GC3 were highly effective at increasing GCL and HMOX-1 anti-oxidant enzymes expression leading to the rapid scavenging of ROS. The antioxidant activity of these oils was explained throughout the activation of NRF2 signaling pathway. Next, we demonstrated that essential oils were able to reduce CD4+ T cell activation which are also involved in inflammatory processes. Conclusions: Our data describe for the first time that chamomile essential oils exerted their anti-inflammatory and antioxidant activity by modulating macrophages and CD4+ T cells-mediate immune response.
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- 2023
42. PP74 - Hydrogen sulfide donors: New potential agents in melanoma treatment.
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Panza, Elisabetta, Armogida, Chiara, De Cicco, Paola, Ercolano, Giuseppe, Scognamiglio, Giosuè, Scafati, Orazio Taglialatela, Cirino, Giuseppe, and Ianaro, Angela
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- *
HYDROGEN sulfide , *MELANOMA treatment , *CHEMICAL potential , *PHARMACY , *PHARMACEUTICAL industry - Published
- 2015
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43. Indicaxanthin from Opuntia Ficus Indica (L. Mill) impairs melanoma cell proliferation, invasiveness, and tumor progression
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Luisa Tesoriere, Rosalia Busà, Alessandro Attanzio, Giuseppe Ercolano, Paola De Cicco, Mario Allegra, M. A. Livrea, Angela Ianaro, Giuseppe Cirino, Mario Allegra, Paola De Cicco, Giuseppe Ercolano, Alessandro Attanzio, Rosalia Busà, Giuseppe Cirino, Luisa Tesoriere, Maria A.Livrea, Angela Ianaro, Allegra, Mario, De Cicco, Paola, Ercolano, Giuseppe, Attanzio, Alessandro, Busà, Rosalia, Cirino, Giuseppe, Tesoriere, Luisa, Livrea, Maria A., and Ianaro, Angela
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0301 basic medicine ,Male ,Skin Neoplasms ,Pyridines ,Pyridine ,Phytochemicals ,Melanoma, Experimental ,Pharmaceutical Science ,Indicaxanthin ,Apoptosis ,Bcl-2, B cell lymphoma gene-2 (Bcl-2) ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Opuntia Ficus Indica (L.Mill) ,Settore BIO/10 - Biochimica ,Drug Discovery ,CXCL1, chemokine (C-X-C motif) ligand 1 ,Melanoma ,NF-κB, nuclear factor kappa B ,MTT, 3-[4,5-dimethyltiazol-2-yl]-2,5-diphenyl tetrazolium bromide ,NF-kappa B ,Opuntia ,Complementary and Alternative Medicine2708 Dermatology ,Betaxanthins ,CXCL1 ,030220 oncology & carcinogenesis ,Molecular Medicine ,PhC, phytochemical ,Growth inhibition ,Human ,Biology ,Phytochemical ,NHEM, normal human epidermal melanocyte ,03 medical and health sciences ,c-FLIP, FLICE-inhibitory protein ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Neoplasm Invasiveness ,Skin Neoplasm ,Cell Proliferation ,Neoplasm Invasivene ,Inflammation ,Pharmacology ,Cell growth ,Animal ,Drug Discovery3003 Pharmaceutical Science ,Apoptosi ,medicine.disease ,Mice, Inbred C57BL ,030104 developmental biology ,Complementary and alternative medicine ,chemistry ,Tumor progression ,List of Abbrevations: AxV-FITC, annexin V-fluorescein isothiocyanate ,Betaxanthin ,Fruit ,Cutaneous melanoma ,Cancer research ,PI, propidium iodide PI - Abstract
Background: A strong, reciprocal crosstalk between inflammation and melanoma has rigorously been demonstrated in recent years, showing how crucial is a pro-inflammatory microenvironment to drive therapy resistance and metastasis. Purpose: We investigated on the effects of Indicaxanthin, a novel, anti-inflammatory and bioavailable phytochemical from Opuntia Ficus Indica fruits, against human melanoma both in vitro and in vivo. Study Design and Methods: The effects of indicaxanthin were evaluated against the proliferation of A375 human melanoma cell line and in a mice model of cutaneous melanoma. Cell proliferation was assessed by MTT assay, apoptosis by Annexin V-Fluorescein Isothiocyanate/Propidium Iodide staining, protein expression by western blotting, melanoma lesions were subcutaneously injected in mice with B16/F10 cells, chemokine release was quantified by ELISA. Results: Data herein presented demonstrate that indicaxanthin effectively inhibits the proliferation of the highly metastatic and invasive A375 cells as shown by growth inhibition, apoptosis induction and cell invasiveness reduction. More interestingly, in vitro data were paralleled by those in vivo showing that indicaxanthin significantly reduced tumor development when orally administered to mice. The results of our study also clarify the molecular mechanisms underlying the antiproliferative effect of indicaxanthin, individuating the inhibition of NF-κB pathway as predominant. Conclusion: In conclusion, we demonstrated that indicaxanthin represents a novel phytochemical able to significantly inhibit human melanoma cell proliferation in vitro and to impair tumor progression in vivo. When considering the resistance of melanoma to the current therapeutical approach and the very limited number of phytochemicals able to partially counteract it, our findings may be of interest to explore indicaxanthin potential in further and more complex melanoma studies in combo therapy, i.e. where different check points of melanoma development are targeted.
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- 2018
44. MicroRNA-143-3p inhibits growth and invasiveness of melanoma cells by targeting cyclooxygenase-2 and inversely correlates with malignant melanoma progression
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Giuseppe Cirino, Chiara Armogida, Angela Ianaro, Giosuè Scognamiglio, Gerardo Botti, Elisabetta Panza, Giuseppe Ercolano, Paola De Cicco, Panza, Elisabetta, Ercolano, Giuseppe, De Cicco, Paola, Armogida, Chiara, Scognamiglio, Giosuè, Botti, Gerardo, Cirino, Giuseppe, and Ianaro, Angela
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0301 basic medicine ,Down-Regulation ,Apoptosis ,medicine.disease_cause ,Biochemistry ,Gene Expression Regulation, Enzymologic ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Cell Line, Tumor ,microRNA ,Biopsy ,Medicine ,Humans ,Neoplasm Invasiveness ,Melanoma ,Cell Proliferation ,Inflammation ,Tumorigenesi ,Pharmacology ,medicine.diagnostic_test ,Malignant melanoma ,business.industry ,miR-143-3p ,Cancer ,medicine.disease ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Cyclooxygenase 2 ,030220 oncology & carcinogenesis ,Cancer research ,Ectopic expression ,Skin cancer ,business ,Carcinogenesis ,Cyclooxygenase-2 (COX-2) - Abstract
Malignant melanoma is one of the most leading form of skin cancer associated with a low patient survival rate. Increasing evidence revealed that microRNAs (miRNAs) play a crucial role in the occurrence and development of several form of cancer including melanoma. In this study, we aimed at investigating the expression and role of miR-143-3p in human malignant melanoma. Our results showed that the expression of miR-143-3p was lower in human melanoma cells, as well as human biopsy specimens, when compared to normal human melanocytes. Ectopic expression of miR-143-3p in human melanoma cells inhibited proliferation, migration, invasion and promoted apoptosis acting through a molecular mechanism that, at least in part, is dependent on inhibition of cyclooxygenase-2 (COX-2) gene. Collectively, these results demonstrate that miR-143-3p could represent at the same time, a new early diagnostic marker and therapeutic target acting as tumor suppressor in melanoma cancer.
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- 2018
45. NMR-based phytochemical analysis of Vitis vinifera cv Falanghina leaves. Characterization of a previously undescribed biflavonoid with antiproliferative activity
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Orazio Taglialatela-Scafati, Martino Forino, Angelita Gambuti, Luigi Moio, Luciana Tartaglione, Giuseppe Ercolano, Paola De Cicco, Angela Ianaro, Tartaglione, Luciana, Gambuti, Angelita, DE CICCO, Paola, Ercolano, Giuseppe, Ianaro, Angela, TAGLIALATELA SCAFATI, Orazio, Moio, Luigi, and Forino, Martino
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0301 basic medicine ,Phytochemicals ,Biology ,Caftaric acid ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Nutraceutical ,Cell Line, Tumor ,Drug Discovery ,Botany ,Biflavonoids ,Humans ,Vitis ,Vitis vinifera ,Melanoma ,Pharmacology ,chemistry.chemical_classification ,030109 nutrition & dietetics ,Molecular Structure ,fungi ,food and beverages ,Plant physiology ,Biflavonoid ,Vitis vinifera, Falanghina cultivar, glycosylated flavonoids, antiproliferative activity ,04 agricultural and veterinary sciences ,General Medicine ,040401 food science ,Antineoplastic Agents, Phytogenic ,Plant Leaves ,chemistry ,Phytochemical ,Italy ,Genetic selection ,Human melanoma - Abstract
Vitis vinifera cv Falanghina is an ancient grape variety of Southern Italy. A thorough phytochemical analysis of the Falanghina leaves was conducted to investigate its specialised metabolite content. Along with already known molecules, such as caftaric acid, quercetin-3-O-β-D-glucopyranoside, quercetin-3-O-β-D-glucuronide, kaempferol- 3-O-β-D-glucopyranoside and kaempferol-3-O-β-D-glucuronide, a previously undescribed biflavonoid was identified. For this last compound, a moderate bioactivity against metastatic melanoma cells proliferation was discovered. This datum can be of some interest to researchers studying human melanoma. The high content in antioxidant glycosylated flavonoids supports the exploitation of grape vine leaves as an inexpensive source of natural products for the food industry and for both pharmaceutical and nutraceutical companies. Additionally, this study offers important insights into the plant physiology, thus prompting possible technological researches of genetic selection based on the vine adaptation to specific pedo-climatic environments.
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- 2018
46. The Hydrogen Sulfide Releasing Molecule Acetyl Deacylasadisulfide Inhibits Metastatic Melanoma
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Angela Ianaro, Orazio Taglialatela-Scafati, Yalda Shokoohinia, Vincenzo Calderone, Elisabetta Panza, Rosa Camerlingo, Chiara Armogida, Giuseppe Pirozzi, Giuseppe Ercolano, Paola De Cicco, Giuseppe Cirino, DE CICCO, Paola, Panza, Elisabetta, Armogida, Chiara, Ercolano, Giuseppe, TAGLIALATELA SCAFATI, Orazio, Shokoohinia, Yalda, Camerlingo, Rosa, Pirozzi, Giuseppe, Calderone, Vincenzo, Cirino, Giuseppe, and Ianaro, Angela
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0301 basic medicine ,MAPK/ERK pathway ,hydrogen sulfide ,03 medical and health sciences ,Apoptosis ,Hydrogen sulfide ,Melanoma ,Metastasis ,Skin cancer ,Pharmacology ,Pharmacology (medical) ,In vivo ,medicine ,melanoma ,metastasis ,Protein kinase B ,Original Research ,skin cancer ,Chemistry ,apoptosis ,medicine.disease ,apoptosi ,In vitro ,XIAP ,030104 developmental biology ,Biochemistry ,Cell culture ,Cancer research ,metastasi - Abstract
Melanoma is the most common form of skin cancer. Given its high mortality, the interest in the search of preventive measures, such as dietary factors, is growing significantly. In this study we tested, in vitro and in vivo, the potential anti-cancer effect of the acetyl deacylasadisulfide (ADA), a vinyl disulfide compound, isolated and purified from asafoetida a foul-smelling oleo gum-resin of dietary and medicinal relevance. ADA markedly suppressed proliferation of human melanoma cell lines by inducing apoptosis. Moreover, treatment of melanoma cells with ADA reduced nuclear translocation and activation of NF-κB, decreased the expression of the anti-apoptotic proteins c-FLIP, XIAP and Bcl-2 and inhibited the phosphorylation and activation of both AKT and ERK proteins, two of the most frequently deregulated pathways in melanoma. Finally, the results obtained in vitro were substantiated by the findings that ADA significantly and dose-dependently reduced lung metastatic foci formation in C57BL/6 mice. In conclusion, our findings suggest that ADA significantly inhibits melanoma progression in vivo and could represent an important lead compound for the development of new anti-metastatic agents.
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- 2017
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47. Role of the cystathionine gamma lyase/hydrogen sulfide pathway in human melanoma progression
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Gerardo Botti, Maria Napolitano, Mariarosaria Bucci, Elisabetta Panza, Giosuè Scognamiglio, Chiara Armogida, Andreas Papapetropoulos, Domenico Germano, Angela Ianaro, Paola De Cicco, Vincenzo Gigantino, Giuseppe Cirino, Panza, Elisabetta, DE CICCO, Paola, Chiara, Armogida, Giosue, Scognamiglio, Vincenzo, Gigantino, Gerardo, Botti, Domenico, Germano, Maria, Napolitano, Andreas, Papapetropoulo, Bucci, Mariarosaria, Cirino, Giuseppe, and Ianaro, Angela
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Skin Neoplasms ,Cystathionine beta-Synthase ,Down-Regulation ,Sulfurtransferase ,Apoptosis ,Dermatology ,Sulfides ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,Cell Line, Tumor ,parasitic diseases ,medicine ,Animals ,Humans ,Gene Silencing ,Hydrogen Sulfide ,Neoplasm Metastasis ,Nevus ,Protein kinase B ,Melanoma ,Cell Proliferation ,chemistry.chemical_classification ,biology ,Chemistry ,Kinase ,Cystathionine gamma-Lyase ,NF-kappa B ,Cell Cycle Checkpoints ,medicine.disease ,Cystathionine beta synthase ,Allyl Compounds ,Gene Expression Regulation, Neoplastic ,Mice, Inbred C57BL ,Enzyme ,Diallyl trisulfide ,Oncology ,Biochemistry ,Sulfurtransferases ,Disease Progression ,Cancer research ,biology.protein ,Female ,hydrogen sulphide ,Signal Transduction - Abstract
In humans, two main metabolic enzymes synthesize hydrogen sulfide (H2 S): cystathionine γ lyase (CSE) and cystathionine β synthase (CBS). A third enzyme, 3-mercaptopyruvate sulfurtransferase (3-MST), synthesizes H2 S in the presence of the substrate 3-mercaptopyruvate (3-MP). The immunohistochemistry analysis performed on human melanoma samples demonstrated that CSE expression was highest in primary tumors, decreased in the metastatic lesions and was almost silent in non-lymph node metastases. The primary role played by CSE was confirmed by the finding that the overexpression of CSE induced spontaneous apoptosis of human melanoma cells. The same effect was achieved using different H2 S donors, the most active of which was diallyl trisulfide (DATS). The main pro-apoptotic mechanisms involved were suppression of nuclear factor-κB activity and inhibition of AKT and extracellular signal-regulated kinase pathways. A proof of concept was obtained in vivo using a murine melanoma model. In fact, either l-cysteine, the CSE substrate, or DATS inhibited tumor growth in mice. In conclusion, we have determined that the l-cysteine/CSE/H2 S pathway is involved in melanoma progression.
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- 2015
48. Pharmacological inhibition of N-Acylethanolamine acid amidase (NAAA) mitigates intestinal fibrosis through modulation of macrophage activity.
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Nanì MF, Pagano E, De Cicco P, Lucariello G, Cattaneo F, Tropeano FP, Cicia D, Amico R, Raucci F, Ercolano G, Maione F, Rinaldi MM, Esposito F, Ammendola R, Luglio G, Capasso R, Makriyannis A, Petrosino S, Borrelli F, Romano B, and Izzo AA
- Abstract
Background and Aims: Intestinal fibrosis, a frequent complication of inflammatory bowel disease, is characterized by stricture formation with no pharmacological treatment to date. N-acylethanolamine acid amidase (NAAA) is responsible of acylethanolamides (AEs, e.g., palmitoylethanolamide and oleoylethanolamide) hydrolysis. Here, we investigated NAAA and AEs signalling in gut fibrosis., Methods: NAAA and AEs signalling were evaluated in human intestinal specimens from stenotic Crohn's diseases (CD) patients. Gut fibrosis was induced by TNBS, monitored by colonoscopy and unascertained by qRT-PCR, histological analyses, and confocal microscopy. Immune cells were analysed in mesenteric lymph nodes by FACS. Colonic fibroblasts were cultured in conditioned media derived from polarized or not bone marrow-derived macrophages (BMDM). IL-23 signalling was evaluated by qRT-PCR, ELISA, FACS, and western blot in BMDM and in lamina propria CX3CR1+ cells., Results: In ileocolonic human CD strictures, increased transcript expression of NAAA was observed with a decrease of its substrates OEA and PEA. NAAA inhibition reduced intestinal fibrosis in vivo, as revealed by decrease in inflammatory parameters, collagen deposition and fibrosis genes, including epithelial to mesenchymal transition. More in-depth studies revealed modulation of the immune response related to IL-23 following NAAA inhibition. The antifibrotic actions of NAAA inhibition are mediated by Mφ and M2 macrophages that indirectly affect fibroblast collagenogenesis. NAAA inhibitor AM9053 normalized IL-23 signalling in BMDM and in lamina propria CX3CR1+ cells., Conclusions: Our findings provide new insights into the pathophysiological mechanism of intestinal fibrosis and identify NAAA as a promising target for the development of therapeutic treatments to alleviate CD fibrosis., (© The Author(s) 2024. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
- Published
- 2024
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