21 results on '"De Bona KS"'
Search Results
2. Protective effect of gallic acid and Syzygium cumini extract against oxidative stress-induced cellular injury in human lymphocytes.
- Author
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De Bona KS, Bonfanti G, Bitencourt PE, da Silva TP, Borges RM, Boligon A, Pigatto A, Athayde ML, and Moretto MB
- Subjects
- Amidines pharmacology, Antioxidants isolation & purification, Cell Culture Techniques, Cell Survival drug effects, Cells, Cultured, Chromatography, High Pressure Liquid, Humans, Lipid Peroxidation drug effects, Lymphocytes pathology, Plant Extracts isolation & purification, Plant Leaves chemistry, Antioxidants pharmacology, Gallic Acid pharmacology, Lymphocytes drug effects, Oxidative Stress drug effects, Plant Extracts pharmacology, Syzygium chemistry
- Abstract
Context: Syzygium cumini (Myrtaceae) presents antioxidant, anti-inflammatory, hypoglycemic and antibacterial effects; however, the cellular and molecular mechanisms of action in the immune system are not yet completely elucidated., Objective: This study evaluates the in vitro effect of gallic acid and aqueous S. cumini leaf extract (ASc) on adenosine deaminase (ADA) and dipeptidyl peptidase IV (DPP-IV) activities, cell viability and oxidative stress parameters in lymphocytes exposed to 2, 2'-azobis-2-amidinopropane dihydrochloride (AAPH)., Materials and Methods: Lymphocytes were incubated with ASc (100 and 500 µg/ml) and gallic acid (50 and 200 µM) at 37 °C for 30 min followed by incubation with AAPH (1 mM) at 37 °C for 2 h. After the incubation time, the lymphocytes were used for determinations of ADA, DPP-IV and lactate dehydrogenase (LDH) activities, lipid peroxidation, protein thiol (P-SH) group levels and cellular viability by colorimetric methods., Results: (i) HPLC fingerprinting of ASc revealed the presence of catechin, epicatechin, rutin, quercitrin, isoquercitrin, quercetin, kaempferol and chlorogenic, caffeic, gallic and ellagic acids; (ii) for the first time, ASc reduced the AAPH-induced increase in ADA activity, but no effect was observed on DPP-IV activity; (iii) ASc increased P-SH groups and cellular viability and decreased LDH activity, but was not able to reduce the AAPH-induced lipid peroxidation; (iv) gallic acid showed less protective effects than ASc., Discussion and Conclusion: ASc affects the purinergic system and may modulate adenosine levels, indicating that the extract of this plant exhibits immunomodulatory properties. ASc also may potentially prevent the cellular injury induced by oxidative stress, highlighting its cytoprotective effects.
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- 2016
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3. Adenosine deaminase, dipeptidyl peptidase-IV activities and lipid peroxidation are increased in the saliva of obese young adult.
- Author
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Chielle EO, Bonfanti G, De Bona KS, Moresco RN, and Moretto MB
- Subjects
- Female, Humans, Insulin Resistance, Male, Obesity enzymology, Young Adult, Adenosine Deaminase metabolism, Dipeptidyl Peptidase 4 metabolism, Lipid Peroxidation, Obesity metabolism, Saliva enzymology
- Abstract
Background: Obesity is the hallmark of the metabolic syndrome representing a major global health problem. It is considered a state of chronic inflammation with minimal exploration of salivary biomarkers. Thus, the intent of the present study was to assess the activities of salivary dipeptidyl peptidase IV (DPP-IV), adenosine deaminase (ADA) and lipid peroxidation in obese young and overweight young subjects., Methods: ADA, DPP-IV activities and lipid peroxidation were investigated in saliva, as well as insulin, glucose, HbA1c, HOMA and anthropometric measurements in 149 young adults, including 54 with normal weight, 27 overweight and 68 obese subjects., Results: Salivary ADA and DPP-IV activities as well as lipid peroxidation were higher in patients with obesity compared to the normal weight group. Correlations between ADA/DPP-IV activities, lipid peroxidation/ADA activity, ADA activity/hip circumference and BMI/weight were observed., Conclusions: Our results indicate that the increase in the salivary ADA and DPP-IV activities as well as in the lipid peroxidation could be related of the regulation to various aspects of adipose tissue function and inflammatory obesity. It is suggested that these salivary biomarkers may be used as biochemical test in clinical abnormalities present in obesity, in the absence of oral inflammatory diseases.
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- 2015
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4. Syzygium cumini is more effective in preventing the increase of erythrocytic ADA activity than phenolic compounds under hyperglycemic conditions in vitro.
- Author
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De Bona KS, Bonfanti G, Bitencourt PE, Cargnelutti LO, da Silva PS, da Silva TP, Zanette RA, Pigatto AS, and Moretto MB
- Subjects
- Chromatography, High Pressure Liquid, Dose-Response Relationship, Drug, Erythrocytes enzymology, Humans, In Vitro Techniques, Adenosine Deaminase blood, Erythrocytes drug effects, Hyperglycemia blood, Phenols pharmacology, Plant Extracts pharmacology, Syzygium chemistry
- Abstract
Syzygium cumini (S. cumini) is a plant known for its antidiabetic properties. The aim of this study was to evaluate the effect of Sc aqueous leaf extract (ASc) on adenosine deaminase (ADA) activity in erythrocytes (RBCs) exposed to high glucose concentrations (30 mM) in vitro. We also investigated the effects of the main phenolic compounds found in ASc (gallic acid, rutin, and chlorogenic acid) and the effects of insulin, caffeine, and dipyridamole, which are substances involved in the adenosine metabolism, on ADA activity in vitro. Blood samples were obtained from healthy volunteers and a suspension of RBCs was used for the determination of ADA activity. The results showed that: (1) the effect of ASc on ADA activity was more significant than the combination of phenolic compounds; (2) insulin, caffeine, or dipyridamole prevented high glucose increase of ADA activity at doses as low as 50 μU/mL, 25 μM, and 1 μM, respectively; (3) the inhibitory effect caused by ASc on erythrocyte ADA activity remained practically the same after the combination of the extract with insulin or caffeine; (4) when RBCs were exposed to ASc plus dipyridamole, this chemical attenuated the effect of ASc on ADA activity, suggesting an antagonism or a competition with ASc by the same site of action. Therefore, ASc was more effective in preventing the increase in ADA activity than phenolic compounds, suggesting that ASc may collaborate to improve endothelial dysfunction, antioxidant, anti-inflammatory, and antithrombotic properties of adenosine by affecting its metabolism. The results of this study help to provide evidence of the empirically supported benefits of the use of S. cumini in diabetes.
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- 2014
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5. Ischemia-modified albumin and inflammatory biomarkers in patients with prostate cancer.
- Author
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Da Silveira RA, Hermes CL, Almeida TC, Bochi GV, De Bona KS, Moretto MB, and Moresco RN
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- Aged, Antioxidants analysis, Biomarkers blood, Case-Control Studies, Humans, Male, Middle Aged, Oxidative Stress, Prostatic Neoplasms immunology, Serum Albumin, Serum Albumin, Human, Biomarkers, Tumor blood, Inflammation Mediators blood, Prostatic Neoplasms blood
- Abstract
Background: Prostate cancer has become a public health problem in many countries and there is evidence which indicates that inflammation and oxidative stress play a key role in the pathogenesis of this disease. Thus, the aim of this study was to evaluate the concentrations of new biomarkers of oxidative stress, ischemia-modified albumin (IMA) and ferric reducing ability of plasma (FRAP), as well as the inflammatory markers in patients with prostate cancer., Methods: CRP, IMA, FRAP, fasting glucose, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, uric acid, creatinine, albumin, AST, ALT, ADA, total PSA (tPSA), free PSA, and proportion of free PSA (fPSA%) were measured in 25 patients with prostate cancer and in 30 healthy subjects., Results: tPSA, CRP, and IMA were significantly higher in patients with prostate cancer. In contrast, fPSA% and FRAP were significantly lower in these patients. However, no significant differences were observed when IMA values were adjusted for serum albumin. Significant correlations were also observed for tPSA and CRP (r = 0.5104, p < 0.001) and for fPSA% and CRP (r = -0.5059, p < 0.001)., Conclusions: We demonstrated that both inflammatory and oxidative processes are increased during prostate cancer and also that there is a reduction of antioxidant defenses in this pathology.
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- 2014
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6. Butyrylcholinesterase and γ-glutamyltransferase activities and oxidative stress markers are altered in metabolic syndrome, but are not affected by body mass index.
- Author
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De Bona KS, Bonfanti G, Bitencourt PE, Cargnelutti LO, da Silva PS, De Lucca L, Pimentel VC, Tatsch E, Gonçalves TL, Premaor M, Moresco RN, and Moretto MB
- Subjects
- Adenosine Deaminase metabolism, Biomarkers metabolism, Body Mass Index, C-Reactive Protein metabolism, Cholesterol blood, Dipeptidyl Peptidase 4 metabolism, Female, Humans, Inflammation immunology, Male, Middle Aged, Nitric Oxide metabolism, Butyrylcholinesterase metabolism, Metabolic Syndrome enzymology, Metabolic Syndrome metabolism, Oxidative Stress immunology, gamma-Glutamyltransferase metabolism
- Abstract
Metabolic syndrome (MetS) leads to changes in enzymatic activities, oxidative and inflammatory parameters. Adenosine deaminase (ADA), dipeptidyl peptidase IV (DPP-IV), butyrylcholinesterase (BuChE) and γ-glutamyltransferase (γ-GT) activities, C-reactive protein (hsCRP) and nitric oxide levels (NOx), as well as oxidative stress markers were analyzed in 39 subjects with MetS and 48 controls. Also, the influence of body mass index (BMI) and anthropometric measurements were evaluated. Disturbances in antioxidant defenses and higher γ-GT and BuChE activities, NOx and hsCRP levels were observed in subjects with MetS. These findings remained associated with MetS after adjustment for BMI, except for hsCRP. ADA was correlated with age, insulin levels and HOMA-IR index in MetS. DPP-IV and total cholesterol (TC), BuChE activity and TC, and VIT C and hsCRP levels also were correlated. The analyzed parameters may reflect the inflammatory state of the MetS, and could contribute to prevention and control of various aspects of this syndrome.
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- 2013
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7. Differential effects of organic and inorganic selenium compounds on adenosine deaminase activity and scavenger capacity in cerebral cortex slices of young rats.
- Author
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Bitencourt PE, Bellé LP, Bonfanti G, Cargnelutti LO, de Bona KS, Silva PS, Abdalla FH, Zanette RA, Guerra RB, Funchal C, and Moretto MB
- Subjects
- Animals, Animals, Newborn, Cell Survival drug effects, Cerebral Cortex enzymology, Cerebral Cortex growth & development, Dose-Response Relationship, Drug, Free Radical Scavengers administration & dosage, In Vitro Techniques, Lipid Peroxidation drug effects, Male, Molecular Structure, Nitric Oxide metabolism, Organoselenium Compounds administration & dosage, Rats, Rats, Wistar, Selenic Acid administration & dosage, Adenosine Deaminase metabolism, Cerebral Cortex drug effects, Free Radical Scavengers pharmacology, Organoselenium Compounds pharmacology, Selenic Acid pharmacology
- Abstract
Selenium (Se) has anti-inflammatory and antioxidant properties and is necessary for the development and normal function of the central nervous system. This study was aimed to compare the in vitro effects of 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one (C21H2HOSe; organoselenium) and sodium selenate (inorganic Se) on adenosine deaminase (ADA) activity, cell viability, lipid peroxidation, scavenger of nitric oxide (NO) and nonprotein thiols (NP-SH) content in the cerebral cortex slices of the young rats. A decrease in ADA activity was observed when the slices were exposed to organoselenium at the concentrations of 1, 10 and 30 µM. The same compound showed higher scavenger capacity of NO than the inorganic compound. Inorganic Se was able to protect against sodium nitroprusside-induced oxidative damage and increased the NP-SH content. Both the compounds displayed distinctive antioxidant capacities and were not cytotoxic for the cerebral cortex slices in the conditions tested. These findings are likely to be related to immunomodulatory and antioxidant properties of this compound.
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- 2013
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8. Lymphocytic enzymes and lipid peroxidation in patients with metabolic syndrome.
- Author
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De Bona KS, Bonfanti G, Cargnelutti LO, Bitencourt PE, da Silva PS, Ceolin R, Pimentel VC, and Moretto MB
- Subjects
- Adult, Biomarkers analysis, Case-Control Studies, Dipeptidyl Peptidase 4 metabolism, Enzyme Activation, Enzyme Assays, Female, Humans, Inflammation enzymology, Insulin metabolism, Male, Metabolic Syndrome diagnosis, Middle Aged, Risk Factors, Thiobarbituric Acid Reactive Substances metabolism, beta-N-Acetyl-Galactosaminidase metabolism, gamma-Glutamyltransferase metabolism, Lipid Peroxidation, Lymphocytes enzymology, Metabolic Syndrome enzymology
- Abstract
Objectives: Metabolic syndrome (MetS) is considered a state of chronic inflammation. This study aimed to ascertain selected parameters of purinergic and cholinergic systems related to glucose metabolism and inflammation, as well as (γ)-glutamyltransferase (GGT) and N-acetyl-b-glucosaminidase (NAG) activities and lipoperoxidation in lymphocytes of patients with MetS., Design and Methods: The adenosine deaminase (ADA), dipeptidyl peptidase IV (DPP-IV), acetylcholinesterase (AChE), GGT and NAG activities, as well as thiobarbituric acid reactive substances (TBARS) levels were investigated in lymphocytes of patients with MetS (n=38) and healthy volunteers (n=41). We also evaluated the insulin levels, anthropometric measurements and routine biochemical analyses., Results: ADA (p<0.05), DPP-IV and AChE (p<0.0001) activities were higher in patients with MetS when compared to the control group. Furthermore, we observed correlations between ADA and DPP-IV activities (p=0.0002; r=0.5945), TBARS levels and ADA (p=0.0021; r=0.5172) and DPP-IV activities (p=0.0022; r=0.5010)., Conclusions: Our findings showed that MetS might cause tissue distress that disturbed lymphocytic ADA, DPP-IV and AChE activities in response to inflammatory stimuli. These alterations evidence clinical abnormalities, since these enzymatic systems are able to regulate several aspects of adipose tissue function and inflammatory state of MetS and could be used successfully both for preventing and for halting the progression of MetS., (Copyright © 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.)
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- 2012
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9. Association between HbA1c and dipeptidyl peptidase IV activity in type 2 diabetes mellitus.
- Author
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Bellé LP, Bitencourt PE, De Bona KS, Moresco RN, and Moretto MB
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- Adenosine Deaminase metabolism, Aged, Body Mass Index, C-Reactive Protein metabolism, Female, Humans, Male, Middle Aged, Regression Analysis, Diabetes Mellitus, Type 2 metabolism, Dipeptidyl Peptidase 4 metabolism, Glycated Hemoglobin metabolism
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- 2012
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10. Expression of CD26 and its association with dipeptidyl peptidase IV activity in lymphocytes of type 2 diabetes patients.
- Author
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Bellé LP, Bitencourt PE, de Bona KS, Zanette RA, Moresco RN, and Moretto MB
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- Acetylglucosaminidase metabolism, Adenosine Deaminase metabolism, Female, Humans, Male, Middle Aged, gamma-Glutamyltransferase metabolism, Diabetes Mellitus, Type 2 enzymology, Dipeptidyl Peptidase 4 metabolism, Gene Expression Regulation, Enzymologic, Lymphocytes enzymology
- Abstract
Immune response and inflammation were suggested to play certain roles in the development and complications of type 2 diabetes mellitus. The main objective of this study was to investigate the CD26 expression and its relationship with adenosine deaminase (ADA), dipeptidyl peptidase IV (DPP-IV), γ-glutamyltransferase (GGT), and N-acetyl-β-glucosaminidase (NAG) activities in lymphocytes of type 2 diabetics (T2DM) patients. These parameters were assessed in 25 T2DM patients and 20 control subjects. We observed a decrease in CD26 expression and a significant increase in the ADA activity in T2DM patients when compared with control subjects. There were no differences between activities of DPP-IV, NAG, and GGT in lymphocytes of T2DM patients and control subjects. Meanwhile, a significant negative correlation was observed between CD26 expression and DPP-IV activity in lymphocytes of T2DM patients. Moreover, a positive correlation was found between DPPIV and ADA activities. The results suggest that the reduction of CD26 expression may be associated in the regulation of DPP-IV in T2DM patients.
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- 2011
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11. Erythrocytic enzymes and antioxidant status in people with type 2 diabetes: beneficial effect of Syzygium cumini leaf extract in vitro.
- Author
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De Bona KS, Bellé LP, Bittencourt PE, Bonfanti G, Cargnelluti LO, Pimentel VC, Ruviaro AR, Schetinger MR, Emanuelli T, and Moretto MB
- Subjects
- Acetylcholinesterase metabolism, Adenosine Deaminase metabolism, Catalase metabolism, Cells, Cultured, Erythrocytes drug effects, Female, Humans, Male, Middle Aged, Oxidative Stress drug effects, Thiobarbituric Acid Reactive Substances metabolism, Antioxidants metabolism, Diabetes Mellitus, Type 2 enzymology, Diabetes Mellitus, Type 2 metabolism, Erythrocytes enzymology, Erythrocytes metabolism, Plant Extracts pharmacology, Plant Leaves chemistry, Syzygium chemistry
- Abstract
The aim of the present study was to investigate the effects of Syzygium cumini leaf extract (ASc), on Adenosine deaminase (ADA) and Acetylcholinesterase (AChE) activities, and also on oxidative stress parameters in erythrocytes hemolysates (RBCs) and erythrocytes membranes (ghosts) from type 2 diabetics patients (Type 2 DM) under in vitro conditions. Non protein thiol groups (NP-SH), AChE, Catalase (CAT) and Superoxide Dismutase (SOD) activities were measure in RBCs. Further, ADA activity, Thiobarbituric Acid-Reactive Substances (TBARS) levels and protein thiol groups (P-SH) were estimated in ghosts. Also, P-SH and Vitamin C (VIT C) were measure in plasma sample. The results demonstrated that ADA and AChE activities, besides TBARS levels were higher in erythrocytes of Type 2 DM, while SOD activity and NP-SH levels were decreased when compared to control group. ASc, in vitro, reduced ADA and AChE activities and some parameters of oxidative stress. Furthermore, we observed correlations between VIT C and P-SH levels, ADA activity and P-SH levels, as well as NP-SH and TBARS levels in diabetics. The results suggest that ASc in vitro is able to promote the reduction of inflammation and oxidative stress parameters, and act against biochemical changes occurring in Diabetes mellitus (DM)., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
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- 2011
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12. δ-Aminolevulinate dehydratase activity in type 2 diabetic patients and its association with lipid profile and oxidative stress.
- Author
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Bonfanti G, Ceolin RB, Valcorte T, De Bona KS, de Lucca L, Gonçalves TL, and Moretto MB
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- Biomarkers, Body Mass Index, Case-Control Studies, Diabetes Mellitus, Type 2 enzymology, Humans, Oxidation-Reduction, Porphobilinogen Synthase antagonists & inhibitors, Diabetes Mellitus, Type 2 metabolism, Lipids blood, Oxidative Stress, Porphobilinogen Synthase metabolism
- Abstract
Objectives: To investigate the activity of δ-Aminolevulinate dehydratase (δ-ALA-D) and its possible relationship with oxidative status, lipid profile, body mass index (BMI) in type 2 diabetics (DM2) patients., Design and Methods: δ-ALA-D activity and reactivation index, as well as markers of oxidative stress and biochemical and anthropometrics parameters were determined in DM2 patients (n = 63) and controls (n = 63)., Results: There was a decreased δ-ALA-D activity and a higher reactivation index (p<0.05) in DM2 patients besides an elevated level of oxidative stress. Disturbances on lipid profile were related to the enzymatic activity and BMI also was correlated with oxidative level in DM2 patients (p<0.05)., Conclusion: There is an association between oxidative stress, abnormalities on lipid profile, distribution of body fat and δ-ALA-D activity inhibition as well as the enzyme is more oxidized in the DM2 suggesting that it would be a good biomarker for assessing prejudice in chronic metabolic processes., (Copyright © 2011 Elsevier B.V. All rights reserved.)
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- 2011
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13. Hypoxic-ischemic brain injury stimulates inflammatory response and enzymatic activities in the hippocampus of neonatal rats.
- Author
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Pimentel VC, Pinheiro FV, De Bona KS, Maldonado PA, da Silva CR, de Oliveira SM, Ferreira J, Bertoncheli CM, Schetinger MR, Da Luz SC, and Moretto MB
- Subjects
- Animals, Animals, Newborn, Astrocytes metabolism, Astrocytes pathology, Hexosaminidases metabolism, Hippocampus injuries, Hypoxia-Ischemia, Brain immunology, Immunohistochemistry, Inflammation metabolism, Inflammation pathology, Male, Neurons metabolism, Neurons pathology, Peroxidase metabolism, Rats, Rats, Wistar, Hippocampus enzymology, Hippocampus pathology, Hypoxia-Ischemia, Brain metabolism, Hypoxia-Ischemia, Brain pathology
- Abstract
Brain damage from neonatal hypoxia-ischemia (HI) plays a major role in neonatal mortality and morbidity. Using the Rice-Vannucci model of HI in rats, we verified that 8 days after HI injury, adenosine deaminase (ADA), N-acetyl-glucosaminidase (NAG) and myeloperoxidase (MPO) activities increased in the left hemisphere hippocampus (HI group); however, the activity of 5'-nucleotidase (5'NT) remained unchanged. In the hematoxylin-eosin analysis (HE), we detected selective and delayed degeneration of hippocampal pyramidal neurons and astroglial reaction accompanied by glial fibrillary acidic protein (GFAP)-positive and vimentin-positive in the immunohistochemistry analysis in the HI group compared with the control group. We observed the selective necrosis of neurons, vascular endothelial proliferation and inflammatory response accompanied by the increase of the key enzyme of adenosine metabolism in the HI group. The increase of ADA activity, despite the 5'NT activity was not altered, indicates the predominance of ADA activity in the postischemic homeostasis of extra cellular adenosine. The presence of leukocytes into the ischemic areas displays the possible importance of the neutrophil-macrophages associated with the increase of MPO and NAG activities 8 days after HI. These findings may contribute to the evaluation of some consequences of the damage caused by neonatal HI., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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14. Protective effects of Syzygium cumini seed extract against methylmercury-induced sistemic toxicity in neonatal rats.
- Author
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Abdalla FH, Bellé LP, Bitencourt PE, De Bona KS, Zanette RA, Boligon AA, Athayde ML, Pigatto AS, and Moretto MB
- Subjects
- Adenosine Deaminase metabolism, Animals, Animals, Newborn, Body Weight drug effects, Cerebral Cortex drug effects, Cerebral Cortex metabolism, Chromatography, High Pressure Liquid, Hippocampus drug effects, Hippocampus metabolism, Kidney drug effects, Kidney metabolism, Liver drug effects, Liver metabolism, Rats, Thiobarbituric Acid Reactive Substances metabolism, Methylmercury Compounds toxicity, Plant Extracts pharmacology, Seeds chemistry, Syzygium chemistry
- Abstract
Syzygium cumini (L.) Skeels (Sc) belongs to the medicinal plants with an important source of phenolic compounds. Sc has been shown to possess antioxidant and anti-inflammatory properties. Methylmercury (MeHg), a highly toxic environmental pollutant, induces oxidative stress and dysfunction in many cell types. This study was aimed to evaluate the effect of aqueous seed extract of Sc (ASc) on MeHg-induced toxicity in rats. Two-day-old rats (P2) received a single dose of MeHg (10 mg/kg) and two doses of ASc (0.9 mg/kg) per os. After two days, the effects of the treatment were investigated in the cerebral cortex, hippocampus, kidney, liver and urine samples. Our results demonstrated that N-acetyl-β-D: -glucosaminidase (NAG) activity in the kidney and urine, the lipid peroxidation levels in the liver and kidney samples, as well as the adenosine deaminase (ADA) activity in the hippocampus, kidney and liver were higher in MeHg-group when compared to the control group. The administration of ASc reverted the toxic effects of MeHg. It is noteworthy to observe that the main compounds present in the ASc, as gallic acid (the major component), chlorogenic acid and rutin, might be the responsible for such benefit, since they were found to display antioxidant properties.
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- 2011
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15. Decrease of adenosine deaminase activity and increase of the lipid peroxidation after acute methotrexate treatment in young rats: protective effects of grape seed extract.
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Pinheiro FV, Pimentel VC, De Bona KS, Scola G, Salvador M, Funchal C, and Moretto MB
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- Alanine Transaminase blood, Animals, L-Lactate Dehydrogenase blood, Male, Rats, Rats, Wistar, Thiobarbituric Acid Reactive Substances metabolism, Uric Acid blood, Uric Acid urine, Adenosine Deaminase metabolism, Grape Seed Extract pharmacology, Lipid Peroxidation drug effects, Methotrexate toxicity
- Abstract
The methotrexate (MTX) is an anti-folate used to treat cancer and some inflammatory diseases. The efficacy of MTX is often limited by its severe toxicity. The present study was undertaken to determine whether Grape seed (Cabernet Sauvignon) extract (GSE) could ameliorate the MTX-induced oxidative injury and the effect on adenosine deaminase activity (ADA) in rats. The rats were pretreated with 50 mg/kg of GSE, i.p., prior to MTX administration (10 mg/kg, i.p.) with a second dose given 4 h and a third dose 16 h after MTX administration. Biochemical parameters were investigated 48 h after the last MTX administration. The administration of MTX increased thiobarbituric acid reactive species (TBARS) levels in hippocampus, kidney and liver, whereas induced a significant decreased in the ADA activity in the cerebral cortex, kidney and liver tissues. MTX administration significantly increased the activity of ALT(alanine aminotransferase) and urea levels and decreased uric acid levels in the serum. Urinary uric acid levels decreased in the MTX group when compared to those of the control group. The GSE along with MTX-administration significantly reversed these parameters toward to near normal. These results indicated that GSE could reduce hepatic and nephritic damage induced by MTX-treatment in young rats therefore having free radical scavenging., (2009 John Wiley & Sons, Ltd.)
- Published
- 2010
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16. Syzygium cumini extract decrease adenosine deaminase, 5'nucleotidase activities and oxidative damage in platelets of diabetic patients.
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De Bona KS, Bellé LP, Sari MH, Thomé G, Schetinger MR, Morsch VM, Boligon A, Athayde ML, Pigatto AS, and Moretto MB
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- 5'-Nucleotidase blood, Adenosine metabolism, Adenosine Deaminase blood, Blood Platelets drug effects, Catalase blood, Catalase metabolism, Diabetes Mellitus, Type 2 blood, Female, Humans, Male, Middle Aged, Plant Leaves chemistry, Superoxide Dismutase blood, Superoxide Dismutase metabolism, Thiobarbituric Acid Reactive Substances metabolism, 5'-Nucleotidase metabolism, Adenosine Deaminase metabolism, Blood Platelets enzymology, Diabetes Mellitus, Type 2 enzymology, Myrtaceae chemistry, Oxidative Stress, Plant Extracts pharmacology
- Abstract
Diabetes mellitus, a chronic metabolic disorder, has assumed epidemic proportions and its long-term complications can have devastating consequences. The oxidative stress in diabetes was greatly increased due to prolonged exposure to hyperglycemia and impairment of oxidant/antioxidant equilibrium. Syzygium cumini is being widely used to treat diabetes by the traditional practitioners over many centuries. Adenosine deaminase (ADA) and 5'-Nucleotidase (5'NT) are enzymes of purine nucleoside metabolism that play an important role in the regulation of adenosine (Ado) levels. In this study, we investigated the effect of Syzygium cumini aqueous leaves extract (ASc) on ADA and 5'NT activities and on parameters of oxidative stress under in vitro conditions, using platelets of patients with Type 2 diabetes mellitus. Platelet-Rich Plasma (PRP) was assayed by ADA, 5'NT, Catalase (CAT), Superoxide Dismutase (SOD) activities and Thiobarbituric acid reactive substances (TBARS) levels. We observed that ADA, 5'NT activities and TBARS levels were significantly higher when compared to the control group, and ASc (100 and 200 μg/mL) prevented these effects. Our study demonstrates that ASc was able to remove oxidant species generated in diabetic conditions and modulates in the Ado levels. Then, ASc may promote a compensatory response in platelet function, improving the susceptibility-induced by the diabetes mellitus., (Copyright © 2010 S. Karger AG, Basel.)
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- 2010
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17. Allium sativum L. extract prevents methyl mercury-induced cytotoxicity in peripheral blood leukocytes (LS).
- Author
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Abdalla FH, Bellé LP, De Bona KS, Bitencourt PE, Pigatto AS, and Moretto MB
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- Acetylglucosamine pharmacology, Adenosine Deaminase metabolism, Antioxidants metabolism, Cell Survival drug effects, Coloring Agents, Humans, Immunity, Cellular drug effects, In Vitro Techniques, Leukocytes enzymology, Oxazines, Plant Extracts pharmacology, Tetrazolium Salts, Thiazoles, Xanthenes, Allium chemistry, Leukocytes drug effects, Methylmercury Compounds antagonists & inhibitors, Methylmercury Compounds toxicity
- Abstract
Adenosine deaminase (ADA) is involved in purine metabolism and plays a significant role in the immune system. The focus of this investigation was to examine the effects of low concentrations of organic mercury on ADA activity in human leukocytes and to investigate the relationship between these effects and cell death. We have examined the protective potential effects of Allium sativum extract (GaE) against Methylmercury (MeHg)-induced cytotoxic effects on human leucocytes under in vitro conditions. MeHg (0.05-10 microM) significantly decreased leukocyte viability (58.97% for MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) and 51.67% for Alamar Blue (AB) and this decrease was positively correlated to the MeHg-induced inhibition of ADA activity. N-acetylcysteine (NAC) and GaE prevented both the MeHg-induced cytotoxic effects on leukocytes according to MTT and AB assays and the effects on the ADA activity. The present results suggest that the protective effects of GaE against MeHg-induced leukocyte damage is related to the removal of oxidant species generated in the presence of MeHg due to the antioxidant efficacy of garlic constituents. It is important to point out that the intense presence of ADA in Leukocyte suspension (LS) highlights the relevant effects in the immune system and in vitro cytotoxicity of MeHg exposure., (Copyright 2009 Elsevier Ltd. All rights reserved.)
- Published
- 2010
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18. Adenosine deaminase activity, lipid peroxidation and astrocyte responses in the cerebral cortex of rats after neonatal hypoxia ischemia.
- Author
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Pimentel VC, Bellé LP, Pinheiro FV, De Bona KS, Da Luz SC, and Moretto MB
- Subjects
- Animals, Animals, Newborn, Astrocytes cytology, Cerebral Cortex pathology, Cerebral Cortex physiopathology, Humans, Hypoxia-Ischemia, Brain complications, Hypoxia-Ischemia, Brain pathology, Hypoxia-Ischemia, Brain physiopathology, Infant, Infant, Newborn, Oxidative Stress, Rats, Rats, Wistar, Adenosine Deaminase metabolism, Astrocytes metabolism, Cerebral Cortex cytology, Cerebral Cortex metabolism, Hypoxia-Ischemia, Brain metabolism, Lipid Peroxidation
- Abstract
Hypoxia ischemia (HI) is a common cause of damage in the fetal and neonatal brain. Lifelong disabilities such as cerebral palsy, epilepsy, behavioral and learning disorders are some of the consequences of brain injury acquired in the perinatal periods. Inflammation and formation of free radicals appear to play key roles in neonatal HI. The aim of this study was to describe the chronological sequence of adenosine deaminase (ADA) activity, the oxidative damage changes and astrocyte response using the classic model of neonatal HI. We observed an increase in the activity of ADA and lipid peroxidation in the cerebral cortex 8 days after neonatal HI. This was accompanied by a GFAP-positive, and the degree of brain damage was determined histochemically by hematoxylin-eosin (HE). Taking into account the important anti-inflammatory role of adenosine, ADA may provide an efficient means for scavenging cell-surrounding adenosine and play an important part in subsequent events of neonatal HI in association with GFAP reactive gliosis. The present investigation showed that neonatal HI causes the increase of free radicals and significant damage in the cerebral cortex. The increase in ADA activity may reflect the activation of the immune system caused by HI because the morphological analysis exhibited a lymphocytic infiltration.
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- 2009
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19. Activity of ectonucleotidases and adenosine deaminase in rats exposed to cigarette smoke.
- Author
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Thomé GR, Mazzanti CM, Ahmed M, Corrêa M, Spanevello RM, Maldonado PA, Luchese C, Cargnelutti D, Morsch VM, Duarte MM, Fiorenza AM, Nogueira CW, De Bona KS, Moretto MB, Da Luz SC, Mazzanti A, and Schetinger MR
- Subjects
- 5'-Nucleotidase blood, Adenosine blood, Animals, Blood Gas Analysis, Blood Platelets enzymology, Carboxyhemoglobin metabolism, Hydrogen-Ion Concentration, Lung enzymology, Lung pathology, Male, Platelet Aggregation drug effects, Pulmonary Fibrosis chemically induced, Pulmonary Fibrosis pathology, Rats, Rats, Wistar, Nicotiana chemistry, Adenosine Deaminase metabolism, Adenosine Triphosphatases metabolism, Tobacco Smoke Pollution adverse effects
- Abstract
Cigarette smoke is a complex mixture of various toxic substances that are capable of initiating oxidative damage and promoting blood platelet alterations. In this study, we investigated the activities of the ectoenzymes NTPDase (ectonucleoside triphosphate diphosphohydrolase, CD39) and 5'-nucleotidase (CD73) in platelets as well as adenosine deaminase (ADA) in the plasma of rats exposed to aged and diluted sidestream smoke during 4 weeks. The rats were divided into two groups: I (control) and II (exposed to smoke). After the exposure period, blood was collected and the platelets and plasma were separated for enzymatic assay. The results demonstrated that NTPDase (with ATP as substrate) and 5'-nucleotidase (AMP as substrate) activities were significantly higher in group II (p < 0.05) as compared to group I, while no significant difference was observed for NTPDase with ADP as substrate. The ADA activity was significantly reduced in group II (p < 0.05) as compared with group I. Platelet aggregation was significantly increased in group II (p < 0.05) as compared with group I. We suggest that these alterations in the activity of enzymes from the purinergic system are associated with an increase in platelet aggregation. However, our study has demonstrated that the organism tries to compensate for this enhanced aggregation by increasing hydrolysis of AMP and reducing hydrolysis of adenosine, a potent inhibitor of aggregation and an important modulator of vascular tone.
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- 2009
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20. Syzygium cumini inhibits adenosine deaminase activity and reduces glucose levels in hyperglycemic patients.
- Author
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Bopp A, De Bona KS, Bellé LP, Moresco RN, and Moretto MB
- Subjects
- Adenosine Deaminase Inhibitors, Adult, Antioxidants metabolism, Brazil, Dipeptidyl Peptidase 4 metabolism, Dose-Response Relationship, Drug, Erythrocytes drug effects, Erythrocytes enzymology, Female, Humans, Hypoglycemic Agents administration & dosage, Hypoglycemic Agents isolation & purification, Hypoglycemic Agents pharmacology, Male, Medicine, Traditional, Middle Aged, Plant Extracts administration & dosage, Plant Leaves, Blood Glucose drug effects, Hyperglycemia drug therapy, Plant Extracts pharmacology, Syzygium chemistry
- Abstract
Syzigium cumini (L.) Skeels from the Myrtaceae family is among the most common medicinal plants used to treat diabetes in Brazil. Leaves, fruits, and barks of S. cumini have been used for their hypoglycemic activity. Adenosine deaminase (ADA) is an important enzyme that plays a relevant role in purine and DNA metabolism, immune responses, and peptidase activity. ADA is suggested to be an important enzyme for modulating the bioactivity of insulin, but its clinical significance in diabetes mellitus (DM) has not yet been proven. In this study, we examined the effect of aqueous leaf extracts of S. cumini (L.) (ASC) on ADA activity of hyperglycemic subjects and the activity of total ADA, and its isoenzymes in serum and erythrocytes. The present study indicates that: (i) the ADA activity in hyperglycemic serum was higher than normoglycemic serum and ADA activity was higher when the blood glucose level was more elevated; (ii) ASC (60-1000 microg/mL) in vitro caused a concentration-dependent inhibition of total ADA activity and a decrease in the blood glucose level in serum; (iii) ADA1 and 2 were reduced both in erythrocytes and in hyperglycemic serum. These results suggest that the decrease of ADA activity provoked by ASC may contribute to control adenosine levels and the antioxidant defense system of red cells and could be related to the complex ADA/DPP-IV-CD26 and the properties of dipeptidyl peptidase IV (DPP-IV) inhibitors which serve as important regulators of blood glucose.
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- 2009
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21. Comparative evaluation of adenosine deaminase activity in cerebral cortex and hippocampus of young and adult rats: effect of garlic extract (Allium sativum L.) on their susceptibility to heavy metal exposure.
- Author
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Bellé LP, De Bona KS, Abdalla FH, Pimentel VC, Pigatto AS, and Moretto MB
- Subjects
- Adenosine Deaminase Inhibitors, Aging drug effects, Animals, Antioxidants pharmacology, Cerebral Cortex enzymology, Dose-Response Relationship, Drug, Hippocampus enzymology, Male, Plant Extracts isolation & purification, Rats, Rats, Wistar, Selenic Acid, Selenium Compounds pharmacology, Sulfhydryl Compounds metabolism, Sulfhydryl Compounds pharmacology, Adenosine Deaminase metabolism, Aging metabolism, Cerebral Cortex drug effects, Garlic chemistry, Hippocampus drug effects, Metals, Heavy toxicity, Methylmercury Compounds toxicity, Plant Extracts pharmacology
- Abstract
Adenosine plays an important neuromodulatory role in the central nervous system, and adenosine deaminase is an important enzyme in the degradation of adenine nucleotides. Methylmercury is the most prevalent form of mercury found in the environment. Methylmercury neurotoxicity has been correlated to the production of reactive oxygen species. In this study, its potential pathogenic effects were investigated in vitro in cerebral cortex and hippocampus of rats. We first observed that adenosine deaminase activity was higher in young rat brains when compared to the 60-day-old rats and was higher in hippocampus when compared to the cortex. Methylmercury (0.1, 1.0, 20 microM) inhibited adenosine deaminase activity in 7- and 60-day-old rats in a concentration-dependent manner. We have demonstrated that methylmercury-induced inhibition was antagonized by garlic alcoholic extract, but sodium selenate did not alter enzyme activity. In addition, glutathione and dithiothreitol restored the methylmercury-induced decrease of adenosine deaminase activity. These results demonstrated that there are age-related changes in adenosine deaminase activity and that thiol agents may contribute to the maintenance of adenosine deaminase activity and may be important in the neuromodulation of adenosine. Garlic alcoholic extract may be effective in reducing the effect of methylmercury-induced adenosine deaminase, which may be due to its sulphur-containing compounds.
- Published
- 2009
- Full Text
- View/download PDF
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