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1. Biological Effects of 25 to 150 GHz Radiation After In Vitro Exposure of Human Fibroblasts: a Comparison of Experimental Results

Author

Franchini, Valeria, Ceccuzzi, Silvio, Doria, Andrea, Gallerano, Gian Piero, Giovenale, Emilio, Ravera, Gian Luca, De Amicis, Andrea, De Sanctis, Stefania, Di Cristofaro, Sara, Regalbuto, Elisa, Coluzzi, Elisa, Marinaccio, Jessica, Sgura, Antonella, Bei, Roberto, Benvenuto, Monica, Modesti, Andrea, Masuelli, Laura, and Lista, Florigio

Published
2018
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2. P53 centrosomal localization diagnoses ataxia-telangiectasia homozygotes and heterozygotes

Author

Prodosmo, Andrea, De Amicis, Andrea, Nistico, Cecilia, Gabriele, Mario, Di Rocco, Giuliana, Monteonofrio, Laura, Piane, Maria, Cundari, Enrico, Chessa, Luciana, and Soddu, Silvia

Subjects
Gene mutations -- Physiological aspects -- Research, Ataxia telangiectasia -- Genetic aspects -- Diagnosis -- Care and treatment -- Research, Genetic screening -- Usage, Health care industry
Abstract

Ataxia-telangiectasia (A-T) is an autosomal recessive neurodegenerative disorder characterized by radiosensitivity, genomic instability, and predisposition to cancer. A-T is caused by biallelic mutations in the ataxia-telangiectasia mutated (ATM) gene, but [...]

Published
2013
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4. Genotoxic Effects in Human Fibroblasts Exposed to Microwave Radiation

Author

Franchini, Valeria, primary, Regalbuto, Elisa, additional, De Amicis, Andrea, additional, De Sanctis, Stefania, additional, Di Cristofaro, Sara, additional, Coluzzi, Elisa, additional, Marinaccio, Jessica, additional, Sgura, Antonella, additional, Ceccuzzi, Silvio, additional, Doria, Andrea, additional, Gallerano, Gian Piero, additional, Giovenale, Emilio, additional, Ravera, Gian Luca, additional, Bei, Roberto, additional, Benvenuto, Monica, additional, Modesti, Andrea, additional, Masuelli, Laura, additional, and Lista, Florigio, additional

Published
2018
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5. Study of the effects of 0.15 terahertz radiation on genome integrity of adult fibroblasts

Author

Franchini, Valeria, primary, De Sanctis, Stefania, additional, Marinaccio, Jessica, additional, De Amicis, Andrea, additional, Coluzzi, Elisa, additional, Di Cristofaro, Sara, additional, Lista, Florigio, additional, Regalbuto, Elisa, additional, Doria, Andrea, additional, Giovenale, Emilio, additional, Gallerano, Gian Piero, additional, Bei, Roberto, additional, Benvenuto, Monica, additional, Masuelli, Laura, additional, Udroiu, Ion, additional, and Sgura, Antonella, additional

Published
2018
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6. RENEB accident simulation exercise

Author

Brzozowska, Beata, Ainsbury, Elizabeth, Baert, Annelot, Beaton-Green, Lindsay, Barrios, Leonardo, Francesc Barquinero, Joan, Bassinet, Celine, Beinke, Christina, Benedek, Anett, Beukes, Philip, Bortolin, Emanuela, Buraczewska, Iwona, Burbidge, Christopher, De Amicis, Andrea, De Angelis, Cinzia, Della Monaca, Sara, Depuydt, Julie, De Sanctis, Stefania, Dobos, Katalin, Domene, Mercedes Moreno, Dominguez, Inmaculada, Facco, Eva, Fattibene, Paola, Frenzel, Monika, Gil, Octavia Monteiro, Gonon, Geraldine, Gregoire, Eric, Gruel, Gaetan, Hadjidekova, Valeria, Hatzi, Vasiliki I., Hristova, Rositsa, Jaworska, Alicja, Kis, Eniko, Kowalska, Maria, Kulka, Ulrike, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Meschini, Roberta, Moertl, Simone, Moquet, Jayne, Noditi, Mihaela, Oestreicher, Ursula, Orta Vazquez, Manuel Luis, Palma, Valentina, Pantelias, Gabriel, Montoro Pastor, Alegria, Patrono, Clarice, Piqueret-Stephan, Laure, Quattrini, Maria Cristina, Regalbuto, Elisa, Ricoul, Michelle, Roch-Lefevre, Sandrine, Roy, Laurence, Sabatier, Laure, Sarchiapone, Lucia, Sebastia, Natividad, Sommer, Sylwester, Sun, Mingzhu, Suto, Yumiko, Terzoudi, Georgia, Trompier, Francois, Vral, Anne, Wilkins, Ruth, Zafiropoulos, Demetre, Wieser, Albrecht, Woda, Clemens, Wojcik, Andrzej, Brzozowska, Beata, Ainsbury, Elizabeth, Baert, Annelot, Beaton-Green, Lindsay, Barrios, Leonardo, Francesc Barquinero, Joan, Bassinet, Celine, Beinke, Christina, Benedek, Anett, Beukes, Philip, Bortolin, Emanuela, Buraczewska, Iwona, Burbidge, Christopher, De Amicis, Andrea, De Angelis, Cinzia, Della Monaca, Sara, Depuydt, Julie, De Sanctis, Stefania, Dobos, Katalin, Domene, Mercedes Moreno, Dominguez, Inmaculada, Facco, Eva, Fattibene, Paola, Frenzel, Monika, Gil, Octavia Monteiro, Gonon, Geraldine, Gregoire, Eric, Gruel, Gaetan, Hadjidekova, Valeria, Hatzi, Vasiliki I., Hristova, Rositsa, Jaworska, Alicja, Kis, Eniko, Kowalska, Maria, Kulka, Ulrike, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Meschini, Roberta, Moertl, Simone, Moquet, Jayne, Noditi, Mihaela, Oestreicher, Ursula, Orta Vazquez, Manuel Luis, Palma, Valentina, Pantelias, Gabriel, Montoro Pastor, Alegria, Patrono, Clarice, Piqueret-Stephan, Laure, Quattrini, Maria Cristina, Regalbuto, Elisa, Ricoul, Michelle, Roch-Lefevre, Sandrine, Roy, Laurence, Sabatier, Laure, Sarchiapone, Lucia, Sebastia, Natividad, Sommer, Sylwester, Sun, Mingzhu, Suto, Yumiko, Terzoudi, Georgia, Trompier, Francois, Vral, Anne, Wilkins, Ruth, Zafiropoulos, Demetre, Wieser, Albrecht, Woda, Clemens, and Wojcik, Andrzej

Abstract

Purpose: The RENEB accident exercise was carried out in order to train the RENEB participants in coordinating and managing potentially large data sets that would be generated in case of a major radiological event. Materials and methods: Each participant was offered the possibility to activate the network by sending an alerting email about a simulated radiation emergency. The same participant had to collect, compile and report capacity, triage categorization and exposure scenario results obtained from all other participants. The exercise was performed over 27 weeks and involved the network consisting of 28 institutes: 21 RENEB members, four candidates and three non-RENEB partners. Results: The duration of a single exercise never exceeded 10 days, while the response from the assisting laboratories never came later than within half a day. During each week of the exercise, around 4500 samples were reported by all service laboratories (SL) to be examined and 54 scenarios were coherently estimated by all laboratories (the standard deviation from the mean of all SL answers for a given scenario category and a set of data was not larger than 3 patient codes). Conclusions: Each participant received training in both the role of a reference laboratory (activating the network) and of a service laboratory (responding to an activation request). The procedures in the case of radiological event were successfully established and tested.

Published
2017
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7. RENEB - Running the European Network of biological dosimetry and physical retrospective dosimetry

Author

Kulka, Ulrike, Abend, Michael, Ainsbury, Elizabeth, Badie, Christophe, Francesc Barquinero, Joan, Barrios, Lleonard, Beinke, Christina, Bortolin, Emanuela, Cucu, Alexandra, De Amicis, Andrea, Dominguez, Inmaculada, Fattibene, Paola, Frovig, Anne Marie, Gregoire, Eric, Guogyte, Kamile, Hadjidekova, Valeria, Jaworska, Alicja, Kriehuber, Ralf, Lindholm, Carita, Lloyd, David, Lumniczky, Katalin, Lyng, Fiona, Meschini, Roberta, Moertl, Simone, Della Monaca, Sara, Gil, Octavia Monteiro, Montoro, Alegria, Moquet, Jayne, Moren, Mercedes, Oestreicher, Ursula, Palitti, Fabrizio, Pantelias, Gabriel, Patrono, Clarice, Piqueret-Stephan, Laure, Port, Matthias, Jesus Prieto, Maria, Quintens, Roel, Ricoul, Michelle, Romm, Horst, Roy, Laurence, Safrany, Geza, Sabatier, Laure, Sebastia, Natividad, Sommer, Sylwester, Terzoudi, Georgia, Testa, Antonella, Thierens, Hubert, Turai, Istvan, Trompier, Francois, Valente, Marco, Vaz, Pedro, Voisin, Philippe, Vral, Anne, Woda, Clemens, Zafiropoulos, Demetre, Wojcik, Andrzej, Kulka, Ulrike, Abend, Michael, Ainsbury, Elizabeth, Badie, Christophe, Francesc Barquinero, Joan, Barrios, Lleonard, Beinke, Christina, Bortolin, Emanuela, Cucu, Alexandra, De Amicis, Andrea, Dominguez, Inmaculada, Fattibene, Paola, Frovig, Anne Marie, Gregoire, Eric, Guogyte, Kamile, Hadjidekova, Valeria, Jaworska, Alicja, Kriehuber, Ralf, Lindholm, Carita, Lloyd, David, Lumniczky, Katalin, Lyng, Fiona, Meschini, Roberta, Moertl, Simone, Della Monaca, Sara, Gil, Octavia Monteiro, Montoro, Alegria, Moquet, Jayne, Moren, Mercedes, Oestreicher, Ursula, Palitti, Fabrizio, Pantelias, Gabriel, Patrono, Clarice, Piqueret-Stephan, Laure, Port, Matthias, Jesus Prieto, Maria, Quintens, Roel, Ricoul, Michelle, Romm, Horst, Roy, Laurence, Safrany, Geza, Sabatier, Laure, Sebastia, Natividad, Sommer, Sylwester, Terzoudi, Georgia, Testa, Antonella, Thierens, Hubert, Turai, Istvan, Trompier, Francois, Valente, Marco, Vaz, Pedro, Voisin, Philippe, Vral, Anne, Woda, Clemens, Zafiropoulos, Demetre, and Wojcik, Andrzej

Abstract

Purpose: A European network was initiated in 2012 by 23 partners from 16 European countries with the aim to significantly increase individualized dose reconstruction in case of large-scale radiological emergency scenarios. Results: The network was built on three complementary pillars: (1) an operational basis with seven biological and physical dosimetric assays in ready-to-use mode, (2) a basis for education, training and quality assurance, and (3) a basis for further network development regarding new techniques and members. Techniques for individual dose estimation based on biological samples and/or inert personalized devices as mobile phones or smart phones were optimized to support rapid categorization of many potential victims according to the received dose to the blood or personal devices. Communication and cross-border collaboration were also standardized. To assure long-term sustainability of the network, cooperation with national and international emergency preparedness organizations was initiated and links to radiation protection and research platforms have been developed. A legal framework, based on a Memorandum of Understanding, was established and signed by 27 organizations by the end of 2015. Conclusions: RENEB is a European Network of biological and physical-retrospective dosimetry, with the capacity and capability to perform large-scale rapid individualized dose estimation. Specialized to handle large numbers of samples, RENEB is able to contribute to radiological emergency preparedness and wider large-scale research projects.

Published
2017
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8. RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA)

Author

Oestreicher, Ursula, Samaga, Daniel, Ainsbury, Elizabeth, Antunes, Ana Catarina, Baeyens, Ans, Barrios, Leonardo, Beinke, Christina, Beukes, Philip, Blakely, William F., Cucu, Alexandra, De Amicis, Andrea, Depuydt, Julie, De Sanctis, Stefania, Di Giorgio, Marina, Dobos, Katalin, Dominguez, Inmaculada, Pham, Ngoc, Espinoza, Marco E., Flegal, Farrah N., Figel, Markus, Garcia, Omar, Gil, Octavia Monteiro, Gregoire, Eric, Guerrero-Carbajal, C., Guclu, Inci, Hadjidekova, Valeria, Hande, Prakash, Kulka, Ulrike, Lemon, Jennifer, Lindholm, Carita, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Maznyk, Nataliya, Meschini, Roberta, M'kacher, Radia, Montoro, Alegria, Moquet, Jayne, Moreno, Mercedes, Noditi, Mihaela, Pajic, Jelena, Radl, Analia, Ricoul, Michelle, Romm, Horst, Roy, Laurence, Sabatier, Laure, Sebastia, Natividad, Slabbert, Jacobus, Sommer, Sylwester, Oliveira, Monica Stuck, Subramanian, Uma, Suto, Yumiko, Tran, Que, Testa, Antonella, Terzoudi, Georgia, Vral, Anne, Wilkins, Ruth, Yanti, LusiYanti, Zafiropoulos, Demetre, Wojcik, Andrzej, Oestreicher, Ursula, Samaga, Daniel, Ainsbury, Elizabeth, Antunes, Ana Catarina, Baeyens, Ans, Barrios, Leonardo, Beinke, Christina, Beukes, Philip, Blakely, William F., Cucu, Alexandra, De Amicis, Andrea, Depuydt, Julie, De Sanctis, Stefania, Di Giorgio, Marina, Dobos, Katalin, Dominguez, Inmaculada, Pham, Ngoc, Espinoza, Marco E., Flegal, Farrah N., Figel, Markus, Garcia, Omar, Gil, Octavia Monteiro, Gregoire, Eric, Guerrero-Carbajal, C., Guclu, Inci, Hadjidekova, Valeria, Hande, Prakash, Kulka, Ulrike, Lemon, Jennifer, Lindholm, Carita, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Maznyk, Nataliya, Meschini, Roberta, M'kacher, Radia, Montoro, Alegria, Moquet, Jayne, Moreno, Mercedes, Noditi, Mihaela, Pajic, Jelena, Radl, Analia, Ricoul, Michelle, Romm, Horst, Roy, Laurence, Sabatier, Laure, Sebastia, Natividad, Slabbert, Jacobus, Sommer, Sylwester, Oliveira, Monica Stuck, Subramanian, Uma, Suto, Yumiko, Tran, Que, Testa, Antonella, Terzoudi, Georgia, Vral, Anne, Wilkins, Ruth, Yanti, LusiYanti, Zafiropoulos, Demetre, and Wojcik, Andrzej

Abstract

Purpose: Two quality controlled inter-laboratory exercises were organized within the EU project 'Realizing the European Network of Biodosimetry (RENEB)' to further optimize the dicentric chromosome assay (DCA) and to identify needs for training and harmonization activities within the RENEB network. Materials and methods: The general study design included blood shipment, sample processing, analysis of chromosome aberrations and radiation dose assessment. After manual scoring of dicentric chromosomes in different cell numbers dose estimations and corresponding 95% confidence intervals were submitted by the participants. Results: The shipment of blood samples to the partners in the European Community (EU) were performed successfully. Outside the EU unacceptable delays occurred. The results of the dose estimation demonstrate a very successful classification of the blood samples in medically relevant groups. In comparison to the 1st exercise the 2nd intercomparison showed an improvement in the accuracy of dose estimations especially for the high dose point. Conclusions: In case of a large-scale radiological incident, the pooling of ressources by networks can enhance the rapid classification of individuals in medically relevant treatment groups based on the DCA. The performance of the RENEB network as a whole has clearly benefited from harmonization processes and specific training activities for the network partners.

Published
2017
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9. RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA)

Author

Oestreicher, Ursula, primary, Samaga, Daniel, additional, Ainsbury, Elizabeth, additional, Antunes, Ana Catarina, additional, Baeyens, Ans, additional, Barrios, Leonardo, additional, Beinke, Christina, additional, Beukes, Philip, additional, Blakely, William F., additional, Cucu, Alexandra, additional, De Amicis, Andrea, additional, Depuydt, Julie, additional, De Sanctis, Stefania, additional, Di Giorgio, Marina, additional, Dobos, Katalin, additional, Dominguez, Inmaculada, additional, Duy, Pham Ngoc, additional, Espinoza, Marco E., additional, Flegal, Farrah N., additional, Figel, Markus, additional, Garcia, Omar, additional, Monteiro Gil, Octávia, additional, Gregoire, Eric, additional, Guerrero-Carbajal, C., additional, Güçlü, İnci, additional, Hadjidekova, Valeria, additional, Hande, Prakash, additional, Kulka, Ulrike, additional, Lemon, Jennifer, additional, Lindholm, Carita, additional, Lista, Florigio, additional, Lumniczky, Katalin, additional, Martinez-Lopez, Wilner, additional, Maznyk, Nataliya, additional, Meschini, Roberta, additional, M’kacher, Radia, additional, Montoro, Alegria, additional, Moquet, Jayne, additional, Moreno, Mercedes, additional, Noditi, Mihaela, additional, Pajic, Jelena, additional, Radl, Analía, additional, Ricoul, Michelle, additional, Romm, Horst, additional, Roy, Laurence, additional, Sabatier, Laure, additional, Sebastià, Natividad, additional, Slabbert, Jacobus, additional, Sommer, Sylwester, additional, Stuck Oliveira, Monica, additional, Subramanian, Uma, additional, Suto, Yumiko, additional, Que, Tran, additional, Testa, Antonella, additional, Terzoudi, Georgia, additional, Vral, Anne, additional, Wilkins, Ruth, additional, Yanti, LusiYanti, additional, Zafiropoulos, Demetre, additional, and Wojcik, Andrzej, additional

Published
2016
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10. RENEB – Running the European Network of biological dosimetry and physical retrospective dosimetry

Author

Kulka, Ulrike, primary, Abend, Michael, additional, Ainsbury, Elizabeth, additional, Badie, Christophe, additional, Barquinero, Joan Francesc, additional, Barrios, Lleonard, additional, Beinke, Christina, additional, Bortolin, Emanuela, additional, Cucu, Alexandra, additional, De Amicis, Andrea, additional, Domínguez, Inmaculada, additional, Fattibene, Paola, additional, Frøvig,, Anne Marie, additional, Gregoire, Eric, additional, Guogyte, Kamile, additional, Hadjidekova, Valeria, additional, Jaworska, Alicja, additional, Kriehuber, Ralf, additional, Lindholm, Carita, additional, Lloyd, David, additional, Lumniczky, Katalin, additional, Lyng, Fiona, additional, Meschini, Roberta, additional, Mörtl, Simone, additional, Della Monaca, Sara, additional, Monteiro Gil, Octávia, additional, Montoro, Alegria, additional, Moquet, Jayne, additional, Moreno, Mercedes, additional, Oestreicher, Ursula, additional, Palitti, Fabrizio, additional, Pantelias, Gabriel, additional, Patrono, Clarice, additional, Piqueret-Stephan, Laure, additional, Port, Matthias, additional, Prieto, María Jesus, additional, Quintens, Roel, additional, Ricoul, Michelle, additional, Romm, Horst, additional, Roy, Laurence, additional, Sáfrány, Géza, additional, Sabatier, Laure, additional, Sebastià, Natividad, additional, Sommer, Sylwester, additional, Terzoudi, Georgia, additional, Testa, Antonella, additional, Thierens, Hubert, additional, Turai, Istvan, additional, Trompier, François, additional, Valente, Marco, additional, Vaz, Pedro, additional, Voisin, Philippe, additional, Vral, Anne, additional, Woda, Clemens, additional, Zafiropoulos, Demetre, additional, and Wojcik, Andrzej, additional

Published
2016
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11. RENEB accident simulation exercise

Author

Brzozowska, Beata, primary, Ainsbury, Elizabeth, additional, Baert, Annelot, additional, Beaton-Green, Lindsay, additional, Barrios, Leonardo, additional, Barquinero, Joan Francesc, additional, Bassinet, Celine, additional, Beinke, Christina, additional, Benedek, Anett, additional, Beukes, Philip, additional, Bortolin, Emanuela, additional, Buraczewska, Iwona, additional, Burbidge, Christopher, additional, De Amicis, Andrea, additional, De Angelis, Cinzia, additional, Della Monaca, Sara, additional, Depuydt, Julie, additional, De Sanctis, Stefania, additional, Dobos, Katalin, additional, Domene, Mercedes Moreno, additional, Domínguez, Inmaculada, additional, Facco, Eva, additional, Fattibene, Paola, additional, Frenzel, Monika, additional, Monteiro Gil, Octávia, additional, Gonon, Géraldine, additional, Gregoire, Eric, additional, Gruel, Gaëtan, additional, Hadjidekova, Valeria, additional, Hatzi, Vasiliki I., additional, Hristova, Rositsa, additional, Jaworska, Alicja, additional, Kis, Enikő, additional, Kowalska, Maria, additional, Kulka, Ulrike, additional, Lista, Florigio, additional, Lumniczky, Katalin, additional, Martínez-López, Wilner, additional, Meschini, Roberta, additional, Moertl, Simone, additional, Moquet, Jayne, additional, Noditi, Mihaela, additional, Oestreicher, Ursula, additional, Orta Vázquez, Manuel Luis, additional, Palma, Valentina, additional, Pantelias, Gabriel, additional, Montoro Pastor, Alegria, additional, Patrono, Clarice, additional, Piqueret-Stephan, Laure, additional, Quattrini, Maria Cristina, additional, Regalbuto, Elisa, additional, Ricoul, Michelle, additional, Roch-Lefevre, Sandrine, additional, Roy, Laurence, additional, Sabatier, Laure, additional, Sarchiapone, Lucia, additional, Sebastià, Natividad, additional, Sommer, Sylwester, additional, Sun, Mingzhu, additional, Suto, Yumiko, additional, Terzoudi, Georgia, additional, Trompier, Francois, additional, Vral, Anne, additional, Wilkins, Ruth, additional, Zafiropoulos, Demetre, additional, Wieser, Albrecht, additional, Woda, Clemens, additional, and Wojcik, Andrzej, additional

Published
2016
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22. Study of the effects of 0.15 terahertz radiation on genome integrity of adult fibroblasts

Author

Valeria, Franchini, Stefania, De Sanctis, Jessica, Marinaccio, Andrea, De Amicis, Elisa, Coluzzi, Sara, Di Cristofaro, Florigio, Lista, Elisa, Regalbuto, Andrea, Doria, Emilio, Giovenale, Gian Piero, Gallerano, Roberto, Bei, Monica, Benvenuto, Laura, Masuelli, Ion, Udroiu, Antonella, Sgura, Franchini, Valeria, DE SANCTIS, Stefania, Marinaccio, Jessica, DE AMICIS, Andrea, Coluzzi, Elisa, Di Cristofaro, Sara, Lista, Florigio, Regalbuto, Elisa, Doria, Andrea, Giovenale, Emilio, Gallerano, Gian Piero, Bei, Roberto, Benvenuto, Monica, Masuelli, Laura, Udroiu, Ion, and Sgura, Antonella

Subjects
Adult, Chromosome Aberrations, Micronucleus Tests, human fibroblasts, Cell Cycle, Telomere Homeostasis, Fibroblasts, Chromosomal malsegregation, DNA damage, Human fibroblasts, Spindle disturbance, THz radiation, Epidemiology, Genetics (clinical), Health, Toxicology and Mutagenesis, Aneuploidy, Settore MED/04, Genomic Instability, Cell Line, spindle disturbance, human fibroblast, chromosomal malsegregation, Humans, Terahertz Radiation
Abstract

The applications of Terahertz (THz) technologies have significantly developed in recent years, and the complete understanding of the biological effects of exposure to THz radiation is becoming increasingly important. In a previous study, we found that THz radiation induced genomic damage in fetal fibroblasts. Although these cells demonstrated to be a useful model, exposure of human foetuses to THz radiation is highly improbable. Conversely, THz irradiation of adult dermal tissues is cause of possible concern for some professional and nonprofessional categories. Therefore, we extended our study to the investigation of the effects of THz radiation on adult fibroblasts (HDF). In this work, the effects of THz exposure on HDF cells genome integrity, cell cycle, cytological ultrastructure and proteins expression were assessed. Results of centromere-negative micronuclei frequencies, phosphorylation of H2AX histone, and telomere length modulation indicated no induction of DNA damage. Concordantly, no changes in the expression of proteins associated with DNA damage sensing and repair were detected. Conversely, our results showed an increase of centromere-positive micronuclei frequencies and chromosomal nondisjunction events, indicating induction of aneuploidy. Therefore, our results indicate that THz radiation exposure may affect genome integrity through aneugenic effects, and not by DNA breakage. Our findings are compared to published studies, and possible biophysical mechanisms are discussed. Environ. Mol. Mutagen. 59:476-487, 2018. © 2018 Wiley Periodicals, Inc.

Published
2018

23. Genotoxic Effects in Human Fibroblasts Exposed to Microwave Radiation

Author

Elisa Regalbuto, Antonella Sgura, Jessica Marinaccio, Valeria Franchini, Laura Masuelli, Andrea De Amicis, Gian Luca Ravera, Florigio Lista, Gian Piero Gallerano, Elisa Coluzzi, Emilio Giovenale, Silvio Ceccuzzi, Monica Benvenuto, Stefania De Sanctis, Roberto Bei, Andrea Modesti, Sara Di Cristofaro, Andrea Doria, Franchini, Valeria, Regalbuto, Elisa, De Amicis, Andrea, De Sanctis, Stefania, Di Cristofaro, Sara, Coluzzi, Elisa, Marinaccio, Jessica, Sgura, Antonella, Ceccuzzi, Silvio, Doria, Andrea, Gallerano, Gian Piero, Giovenale, Emilio, Ravera, Gian Luca, Bei, Roberto, Benvenuto, Monica, Modesti, Andrea, Masuelli, Laura, and Lista, Florigio

Subjects
0301 basic medicine, Radiology, Nuclear Medicine and Imaging, Epidemiology, Health, Toxicology and Mutagenesis, Aneuploidy, Micronuclei, Histones, Direct DNA damage, Nuclear Medicine and Imaging, Fetu, Cells, Cultured, Cultured, Micronucleus Tests, Histone, Health, Micronucleus test, Fibroblast, electromagnetic fields, genetic effects, radiation, microwaves, radiation, nonionizing, genetic effects, Comet Assay, Radiology, Human, Adult, microwave, DNA damage, Cells, Biology, 03 medical and health sciences, Fetus, electromagnetic field, medicine, Humans, Radiology, Nuclear Medicine and imaging, Toxicology and Mutagenesis, Micronuclei, Chromosome-Defective, Settore MED/04 - Patologia Generale, DNA Damage, Fibroblasts, Microwaves, medicine.disease, Comet assay, radiation, 030104 developmental biology, nonionizing, Cell culture, Apoptosis, Cancer research, biology.protein, Micronucleus Test, Chromosome-Defective
Abstract

In the last decades, technological development has led to an increasing use of devices and systems based on microwave radiation. The increased employment of these devices has elicited questions about the potential long-term health consequences associated with microwave radiation exposure. From this perspective, biological effects of microwave radiation have been the focus of many studies, but the reported scientific data are unclear and contradictory. The aim of this study is to evaluate the potential genotoxic and cellular effects associated with in vitro exposure of human fetal and adult fibroblasts to microwave radiation at the frequency of 25 GHz. For this purpose, several genetic and biological end points were evaluated. Results obtained from comet assay, phosphorylation of H2AX histone, and antikinetochore antibody (CREST)-negative micronuclei frequency excluded direct DNA damage to human fetal and adult fibroblasts exposed to microwaves. No induction of apoptosis or changes in prosurvival signalling proteins were detected. Moreover, CREST analysis showed for both the cell lines an increase in the total number of micronuclei and centromere positive micronuclei in exposed samples, indicating aneuploidy induction due to chromosome loss.

Published
2018

24. Biological Effects of 25 to 150 GHz Radiation After In Vitro Exposure of Human Fibroblasts: a Comparison of Experimental Results

Author

Laura Masuelli, Gian Piero Gallerano, Elisa Coluzzi, Roberto Bei, Andrea Modesti, Gian Luca Ravera, Jessica Marinaccio, Valeria Franchini, Stefania De Sanctis, Elisa Regalbuto, Antonella Sgura, Silvio Ceccuzzi, Andrea De Amicis, Monica Benvenuto, Emilio Giovenale, Florigio Lista, Sara Di Cristofaro, Andrea Doria, Franchini, Valeria, Ceccuzzi, Silvio, Doria, Andrea, Gallerano, Gian Piero, Giovenale, Emilio, Ravera, Gian Luca, De Amicis, Andrea, De Sanctis, Stefania, Di Cristofaro, Sara, Regalbuto, Elisa, Coluzzi, Elisa, Marinaccio, Jessica, Sgura, Antonella, Bei, Roberto, Benvenuto, Monica, Modesti, Andrea, Masuelli, Laura, and Lista, Florigio

Subjects
0301 basic medicine, Genome integrity, Terahertz, Biological effects, Millimeter waves, Condensed Matter Physic, Radiation, 03 medical and health sciences, 0302 clinical medicine, Fibroblasts, Human primary cells, In vitro exposure, Non-ionizing radiation, Instrumentation, Condensed Matter Physics, Electrical and Electronic Engineering, Human primary cell, Biological effect, Pulsed radiation, Settore MED/04 - Patologia Generale, Chemistry, Millimeter wave, Cell cycle, Cell biology, 030104 developmental biology, 030220 oncology & carcinogenesis, Human fetal, Ultrastructure, Fibroblast
Abstract

In this paper, we present a comprehensive discussion of the results obtained after in vitro exposure of human fetal fibroblasts and human adult fibroblasts to pulsed radiation in a wide band between 100 and 150 GHz and to continuous wave radiation at 25 GHz. In order to assess potential effects of exposure, the genome integrity, cell cycle, cytological ultrastructure, and proteins expression were evaluated.

Published
2018

25. RENEB accident simulation exercise.

Author

Brzozowska B, Ainsbury E, Baert A, Beaton-Green L, Barrios L, Barquinero JF, Bassinet C, Beinke C, Benedek A, Beukes P, Bortolin E, Buraczewska I, Burbidge C, De Amicis A, De Angelis C, Della Monaca S, Depuydt J, De Sanctis S, Dobos K, Domene MM, Domínguez I, Facco E, Fattibene P, Frenzel M, Monteiro Gil O, Gonon G, Gregoire E, Gruel G, Hadjidekova V, Hatzi VI, Hristova R, Jaworska A, Kis E, Kowalska M, Kulka U, Lista F, Lumniczky K, Martínez-López W, Meschini R, Moertl S, Moquet J, Noditi M, Oestreicher U, Orta Vázquez ML, Palma V, Pantelias G, Montoro Pastor A, Patrono C, Piqueret-Stephan L, Quattrini MC, Regalbuto E, Ricoul M, Roch-Lefevre S, Roy L, Sabatier L, Sarchiapone L, Sebastià N, Sommer S, Sun M, Suto Y, Terzoudi G, Trompier F, Vral A, Wilkins R, Zafiropoulos D, Wieser A, Woda C, and Wojcik A

Subjects
Europe, Disaster Planning organization & administration, Radiation Monitoring methods, Radioactive Hazard Release, Radiobiology education, Safety Management organization & administration, Triage organization & administration
Abstract

Purpose: The RENEB accident exercise was carried out in order to train the RENEB participants in coordinating and managing potentially large data sets that would be generated in case of a major radiological event., Materials and Methods: Each participant was offered the possibility to activate the network by sending an alerting email about a simulated radiation emergency. The same participant had to collect, compile and report capacity, triage categorization and exposure scenario results obtained from all other participants. The exercise was performed over 27 weeks and involved the network consisting of 28 institutes: 21 RENEB members, four candidates and three non-RENEB partners., Results: The duration of a single exercise never exceeded 10 days, while the response from the assisting laboratories never came later than within half a day. During each week of the exercise, around 4500 samples were reported by all service laboratories (SL) to be examined and 54 scenarios were coherently estimated by all laboratories (the standard deviation from the mean of all SL answers for a given scenario category and a set of data was not larger than 3 patient codes)., Conclusions: Each participant received training in both the role of a reference laboratory (activating the network) and of a service laboratory (responding to an activation request). The procedures in the case of radiological event were successfully established and tested.

Published
2017
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26. RENEB - Running the European Network of biological dosimetry and physical retrospective dosimetry.

Author

Kulka U, Abend M, Ainsbury E, Badie C, Barquinero JF, Barrios L, Beinke C, Bortolin E, Cucu A, De Amicis A, Domínguez I, Fattibene P, Frøvig AM, Gregoire E, Guogyte K, Hadjidekova V, Jaworska A, Kriehuber R, Lindholm C, Lloyd D, Lumniczky K, Lyng F, Meschini R, Mörtl S, Della Monaca S, Monteiro Gil O, Montoro A, Moquet J, Moreno M, Oestreicher U, Palitti F, Pantelias G, Patrono C, Piqueret-Stephan L, Port M, Prieto MJ, Quintens R, Ricoul M, Romm H, Roy L, Sáfrány G, Sabatier L, Sebastià N, Sommer S, Terzoudi G, Testa A, Thierens H, Turai I, Trompier F, Valente M, Vaz P, Voisin P, Vral A, Woda C, Zafiropoulos D, and Wojcik A

Subjects
Emergencies, Europe, Humans, Organizational Objectives, Radiation Exposure analysis, Radiation Exposure prevention & control, Radioactive Hazard Release prevention & control, Biological Assay methods, Disaster Planning organization & administration, Radiation Injuries prevention & control, Radiation Monitoring methods, Radiation Protection methods, Safety Management organization & administration
Abstract

Purpose: A European network was initiated in 2012 by 23 partners from 16 European countries with the aim to significantly increase individualized dose reconstruction in case of large-scale radiological emergency scenarios., Results: The network was built on three complementary pillars: (1) an operational basis with seven biological and physical dosimetric assays in ready-to-use mode, (2) a basis for education, training and quality assurance, and (3) a basis for further network development regarding new techniques and members. Techniques for individual dose estimation based on biological samples and/or inert personalized devices as mobile phones or smart phones were optimized to support rapid categorization of many potential victims according to the received dose to the blood or personal devices. Communication and cross-border collaboration were also standardized. To assure long-term sustainability of the network, cooperation with national and international emergency preparedness organizations was initiated and links to radiation protection and research platforms have been developed. A legal framework, based on a Memorandum of Understanding, was established and signed by 27 organizations by the end of 2015., Conclusions: RENEB is a European Network of biological and physical-retrospective dosimetry, with the capacity and capability to perform large-scale rapid individualized dose estimation. Specialized to handle large numbers of samples, RENEB is able to contribute to radiological emergency preparedness and wider large-scale research projects.

Published
2017
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27. RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA).

Author

Oestreicher U, Samaga D, Ainsbury E, Antunes AC, Baeyens A, Barrios L, Beinke C, Beukes P, Blakely WF, Cucu A, De Amicis A, Depuydt J, De Sanctis S, Di Giorgio M, Dobos K, Dominguez I, Duy PN, Espinoza ME, Flegal FN, Figel M, Garcia O, Monteiro Gil O, Gregoire E, Guerrero-Carbajal C, Güçlü İ, Hadjidekova V, Hande P, Kulka U, Lemon J, Lindholm C, Lista F, Lumniczky K, Martinez-Lopez W, Maznyk N, Meschini R, M'kacher R, Montoro A, Moquet J, Moreno M, Noditi M, Pajic J, Radl A, Ricoul M, Romm H, Roy L, Sabatier L, Sebastià N, Slabbert J, Sommer S, Stuck Oliveira M, Subramanian U, Suto Y, Que T, Testa A, Terzoudi G, Vral A, Wilkins R, Yanti L, Zafiropoulos D, and Wojcik A

Subjects
Biological Assay standards, Europe, Humans, Lymphocytes radiation effects, Radiation Monitoring standards, Reproducibility of Results, Sensitivity and Specificity, Biological Assay methods, Chromosome Aberrations radiation effects, Micronucleus Tests methods, Quality Assurance, Health Care, Radiation Exposure analysis, Radiation Monitoring methods
Abstract

Purpose: Two quality controlled inter-laboratory exercises were organized within the EU project 'Realizing the European Network of Biodosimetry (RENEB)' to further optimize the dicentric chromosome assay (DCA) and to identify needs for training and harmonization activities within the RENEB network., Materials and Methods: The general study design included blood shipment, sample processing, analysis of chromosome aberrations and radiation dose assessment. After manual scoring of dicentric chromosomes in different cell numbers dose estimations and corresponding 95% confidence intervals were submitted by the participants., Results: The shipment of blood samples to the partners in the European Community (EU) were performed successfully. Outside the EU unacceptable delays occurred. The results of the dose estimation demonstrate a very successful classification of the blood samples in medically relevant groups. In comparison to the 1st exercise the 2nd intercomparison showed an improvement in the accuracy of dose estimations especially for the high dose point., Conclusions: In case of a large-scale radiological incident, the pooling of ressources by networks can enhance the rapid classification of individuals in medically relevant treatment groups based on the DCA. The performance of the RENEB network as a whole has clearly benefited from harmonization processes and specific training activities for the network partners.

Published
2017
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28. Biological effects of in vitro THz radiation exposure in human foetal fibroblasts.

Author

De Amicis A, Sanctis SD, Cristofaro SD, Franchini V, Lista F, Regalbuto E, Giovenale E, Gallerano GP, Nenzi P, Bei R, Fantini M, Benvenuto M, Masuelli L, Coluzzi E, Cicia C, and Sgura A

Subjects
Aneuploidy, Apoptosis radiation effects, Cell Survival radiation effects, Cells, Cultured, Centromere genetics, DNA Damage, Fibroblasts metabolism, Foreskin cytology, Foreskin embryology, Histones metabolism, Humans, In Vitro Techniques, Male, Phosphorylation, Terahertz Radiation, Actins metabolism, Centromere radiation effects, Chromosome Segregation radiation effects, Fibroblasts radiation effects, Micronuclei, Chromosome-Defective statistics & numerical data
Abstract

In recent years, terahertz (THz) radiation has been widely used in a variety of applications: medical, security, telecommunications and military areas. However, few data are available on the biological effects of this type of electromagnetic radiation and the reported results, using different genetic or cellular assays, are quite discordant. This multidisciplinary study focuses on potential genotoxic and cytotoxic effects, evaluated by several end-points, associated with THz radiation. For this purpose, in vitro exposure of human foetal fibroblasts to low frequency THz radiation (0.1-0.15THz) was performed using a Compact Free Electron Laser. We did not observe an induction of DNA damage evaluated by Comet assay, phosphorylation of H2AX histone or telomere length modulation. In addiction, no induction of apoptosis or changes in pro-survival signalling proteins were detected. Moreover, our results indicated an increase in the total number of micronuclei and centromere positive micronuclei induction evaluated by CREST analysis, indicating that THz radiation could induce aneugenic rather than clastogenic effects, probably leading to chromosome loss. Furthermore, an increase of actin polymerization observed by ultrastructural analysis after THz irradiation, supports the hypothesis that an abnormal assembly of spindle proteins could lead to the observed chromosomal malsegregation., (Copyright © 2015 Elsevier B.V. All rights reserved.)

Published
2015
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