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Your search keyword '"Davis-Harrison, Rebecca L."' showing total 18 results
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18 results on '"Davis-Harrison, Rebecca L."'

4. Data for: 'Peptide Isolation via Spray Drying: Particle Formation, Process Design and Implementation for the Production of Spray Dried Glucagon'

Author

Doerr, Frederik, Burns, Lee J., Lee, Becky, Hinds, Jeremy, Davis-Harrison, Rebecca L., Frank, Scott A., and Florence, Alastair

Subjects
Peptide Formulation, Psychrometric Process Model, Spray Drying, Droplet Drying, Process Development
Abstract

This is a collection of data and methodologies used in the following publication. Public access to the dataset is currently under embargo until 01/01/2021. Expressions of interest can be made via the contact email: researchdataproject@strath.ac.uk. Further details on the data can be found in the README file provided. Title: Peptide Isolation via Spray Drying: Particle Formation, Process Design and Implementation for the Production of Spray Dried Glucagon Authors: Frederik J. S. Doerr, Lee J. Burns, Becky Lee, Jeremy Hinds, Rebecca L. Davis-Harrison, Scott A. Frank, Alastair J. Florence Journal: Pharmaceutical Research Accepted Date: 28/09/2020 DOI: https://doi.org/10.1007/s11095-020-02942-5 Abstract: Purpose Spray drying plays an important role in the pharmaceutical industry for product development of sensitive bio-pharmaceutical formulations. Process design, implementation and optimisation require in-depth knowledge of process-product interactions. Here, an integrated approach for the rapid, early-stage spray drying process development of trehalose and glucagon on lab-scale is presented. Methods Single droplet drying experiments were used to investigate the particle formation process. Process implementation was supported using in-line process analytical technology within a data acquisition framework recording temperature, humidity, pressure and feed rate. During process implementation, off-line product characterisation provided additional information on key product properties related to residual moisture, solid state structure, particle size/morphology and peptide fibrillation/degradation. Results A psychrometric process model allowed the identification of feasible operating conditions for spray drying trehalose, achieving high yields of up to 84.67%, and significantly reduced levels of residual moisture and particle agglomeration compared to product obtained during non-optimal drying. The process was further translated to produce powders of glucagon and glucagon-trehalose formulations with yields of >83.24%. Extensive peptide aggregation or degradation was not observed. Conclusions The presented data-driven process development concept can be applied to address future isolation problems on lab-scale and facilitate a systematic implementation of spray drying for the manufacturing of sensitive bio-pharmaceutical formulations.

Published
2020
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5. T cell receptor binding transition states and recognition of peptide/MHC

Author

Davis-Harrison, Rebecca L., Insaidoo, Francis K., and Baker, Brian M.

Subjects
Antigen receptors, T cell -- Structure, Antigen receptors, T cell -- Chemical properties, Major histocompatibility complex -- Structure, Major histocompatibility complex -- Research, Binding proteins -- Structure, Binding proteins -- Research, Protein folding -- Research, T cells -- Receptors, T cells -- Structure, T cells -- Chemical properties, Biological sciences, Chemistry
Abstract

A study analyzes the T cell receptor (TCR) recognition of a peptide bound and presented by a major histocompatibility complex (MHC) molecule, which normally comes about in two steps. The results of the analysis were of great help and will be definitely useful in understanding the nature of the TCR binding transition states, which makes it recognize the composite peptide/MHC surface.

Published
2007

6. Phosphoramidate end labeling of inorganic polyphosphates: facile manipulation of polyphosphate for investigating and modulating its biological activities

Author

Choi, Sharon H., Collins, Julie N.R., Smith, Stephanie A., Davis-Harrison, Rebecca L., Rienstra, Chad M., and Morrissey, James H.

Subjects
Amines -- Chemical properties, Biotin -- Structure, Biotin -- Chemical properties, Nuclear magnetic resonance spectroscopy -- Usage, Phosphates -- Chemical properties, Phosphates -- Structure, Protein binding -- Analysis, Biological sciences, Chemistry
Abstract

[Super 31]P NMR was employed to establish that EDAC (1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide)-mediated reactions between primary amines and polyphosphate result in phosphoramidate linkages with the terminal phosphate groups. The findings from the study provide new pathways for the entire armamentarium of protein chemistry to modifying polyphosphate and conduct future studies to understand its biological roles.

Published
2010

10. Molecular Determinants of Phospholipid Synergy in Blood Clotting

Author

Tavoosi, Narjes, primary, Davis-Harrison, Rebecca L., additional, Pogorelov, Taras V., additional, Ohkubo, Y. Zenmei, additional, Arcario, Mark J., additional, Clay, Mary C., additional, Rienstra, Chad M., additional, Tajkhorshid, Emad, additional, and Morrissey, James H., additional

Published
2011
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15. Unraveling a Hotspot for TCR Recognition on HLA-A2: Evidence Against the Existence of Peptide-independent TCR Binding Determinants

Author

Gagnon, Susan J., primary, Borbulevych, Oleg Y., additional, Davis-Harrison, Rebecca L., additional, Baxter, Tiffany K., additional, Clemens, John R., additional, Armstrong, Kathryn M., additional, Turner, Richard V., additional, Damirjian, Marale, additional, Biddison, William E., additional, and Baker, Brian M., additional

Published
2005
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