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People with HIV (PWH) appear to be at higher risk for suboptimal pathogen responses and for worse COVID-19 outcomes, but the effects of host factors and COVID-19 on the humoral repertoire remain unclear. We assessed the antibody isotype/subclass and Fc-receptor binding Luminex arrays of non-SARS-CoV-2 and SARS-CoV-2 humoral responses among antiretroviral therapy-treated (ART-treated) PWH. Among the entire cohort, COVID-19 infection was associated with higher cytomegalovirus (CMV) responses (vs. the COVID- cohort ), potentially signifying increased susceptibility or a consequence of persistent inflammation. Among the COVID+ participants, (a) higher BMI was associated with a striking amplification of SARS-CoV-2 responses, suggesting exaggerated inflammatory responses, and (b) lower nadir CD4 was associated with higher SARS-CoV-2 IgM and FcγRIIB binding capacity, indicating poorly functioning extrafollicular and inhibitory responses. Among the COVID-19- participants, female sex, older age, and lower nadir CD4 were associated with unique repertoire shifts. In this first comprehensive assessment of the humoral repertoire in a global cohort of PWH, we identify distinct SARS-CoV-2-specific humoral immune profiles among PWH with obesity or lower nadir CD4+ T cell count, underlining plausible mechanisms associated with worse COVID-19-related outcomes in this setting. Host factors associated with the humoral repertoire in the COVID-19- cohort enhance our understanding of these important shifts among PWH.

The National Microbiome Data Collaborative (NMDC) Data Portal (https://data.microbiomedata.org) supports microbiome multi-omics data exploration and access through an integrated, distributed data framework aligned with the FAIR (Findable, Accessible, Interoperable and Reusable) data principles (1). The NMDC Data Portal currently hosts 10.2 terabytes of multi-omics microbiome data, spanning five data types (metagenomes, metatranscriptomes, metaproteomes, metabolomes, and natural organic matter characterizations), generated at two Department of Energy User Facilities, the Joint Genome Institute (JGI) at Lawrence Berkeley National Laboratory (LBNL) and the Environmental Molecular Systems Laboratory (EMSL) at Pacific Northwest National Laboratory (PNNL). A flexible data schema (https://github.com/microbiomedata/nmdc-schema) leveraging community-driven standards underpins how data is managed and integrated. Annotated multi-omic data products are produced by the NMDC workflows and linked through common biosamples to enable search capabilities based on environmental context, instrumentation, and functional attributes. As a pilot system, the NMDC Data Portal offers download capabilities and several search components, including interactive geographic visualization of samples; environmental classification distribution visualized through an interactive Sankey diagram; time-series slider to select longitudinal samples of interest; and an upset plot displaying the number of multi-omics data generated from the same biosample within a study.

Cornelia de Lange syndrome (CdLS) is a dominant multisystemic malformation syndrome due to mutations in five genes-NIPBL, SMC1A, HDAC8, SMC3, and RAD21. The characteristic facial dysmorphisms include microcephaly, arched eyebrows, synophrys, short nose with depressed bridge and anteverted nares, long philtrum, thin lips, micrognathia, and hypertrichosis. Most affected individuals have intellectual disability, growth deficiency, and upper limb anomalies. This study looked at individuals from diverse populations with both clinical and molecularly confirmed diagnoses of CdLS by facial analysis technology. Clinical data and images from 246 individuals with CdLS were obtained from 15 countries. This cohort included 49% female patients and ages ranged from infancy to 37 years. Individuals were grouped into ancestry categories of African descent, Asian, Latin American, Middle Eastern, and Caucasian. Across these populations, 14 features showed a statistically significant difference. The most common facial features found in all ancestry groups included synophrys, short nose with anteverted nares, and a long philtrum with thin vermillion of the upper lip. Using facial analysis technology we compared 246 individuals with CdLS to 246 gender/age matched controls and found that sensitivity was equal or greater than 95% for all groups. Specificity was equal or greater than 91%. In conclusion, we present consistent clinical findings from global populations with CdLS while demonstrating how facial analysis technology can be a tool to support accurate diagnoses in the clinical setting. This work, along with prior studies in this arena, will assist in earlier detection, recognition, and treatment of CdLS worldwide.

After realizing the difficulty educators face with integrating the crosscutting concepts (CCCs) from the Next Generation Science Standards into their lessons and noticing missed opportunities for caregivers to engage children in scientific thinking, we posited that if the CCCs are presented in a more accessible way then children will have more opportunities to engage in sensemaking in science. This lesson is the thinking and learning from our attempt to bring this wondering to life. We are a museum educator, a teacher educator, and a first-grade teacher. We developed and taught this lesson with the hope that it engages all students in rich thinking by using a small, manageable lesson as an entry point to begin this work regardless of students' levels of conceptual understanding. This lesson focuses on patterns in students' everyday lives, particularly on the schoolyard. It demonstrates expansive teaching practice and is accessible to all learners and educators by centering CCCs in a decontextualized manner and using elements of photovoice to highlight students' thinking. We describe the lesson we taught using the 5E learning cycle format and provide reflections and recommendations for educators. [ABSTRACT FROM AUTHOR]

Research of preservice Black male teachers' experiences in teacher education programs is limited in general and specifically at Historically Black Colleges and Universities (HBCUs). Moreover, there is little research about the initiatives established to support Black men in teacher education programs, such as undergraduate research experiences. Thus, this participatory action research project uses African American male theory and the scholar identity model to examine how an undergraduate research experience about Black male teachers helped to enhance preservice teachers' unique identities as Black men, teachers, and researchers. The data corpus for this study consists of individual and focus group interviews, video and audio recorded research sessions, pictures, artifacts, and documents. The findings illustrate how the three participants' self-proclaimed identities were enhanced and further developed through the research project. This study offers recommendations on how to support preservice Black male teachers in teacher education programs and undergraduate research experiences.

Building on the theoretical frameworks of both Charles Mills and Juliet Hooker I center race within abjection theory to demonstrate how the lack of concern about the pain and suffering of racial minorities is a link between critical race and abjection theory. The central problematic of this paper is racial abjection—how race creates an altered conceptualization of abjection and what this means for Blacks within the polis. Racial abjection is a powerful mythological, psychological, and physical response to the Black body and Black sexuality. This is the ability and desire of Whites to witness Black pain and suffering. I discuss the relationship among racial abjection, the Black body and Black sexuality. Then I detail the effects of racial abjection on Black masculinity and femininity. Lastly, I offer (dis)identification as a possible starting point for counter-conceptualizing Black identity. [ABSTRACT FROM AUTHOR]