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Your search keyword '"Dauphars, Danielle J."' showing total 11 results
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11 results on '"Dauphars, Danielle J."'

7. Antibody Response and Therapy in COVID-19 Patients: Significance in Vaccine Development

Author

Lu, Ligong, Zhang, Hui, Zhan, Meixiao, Jiang, Jun, Yin, Hua, Dauphars, Danielle J., Li, Shi-You, Li, Yong, and He, You-Wen

Subjects
viruses, other
Abstract

The newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions of people and caused tremendous morbidity and mortality worldwide. Effective treatment for coronavirus disease 2019 (COVID-19) due to SARS-CoV-2 infection is lacking and different therapeutic strategies are under testing. Host humoral and cellular immunity to SARS-CoV-2 infection is a critical determinant for patients’ outcome. SARS-CoV-2 infection results in seroconversion and production of anti-SARS-CoV-2 antibodies. The antibodies may suppress viral replication through neutralization but also might also participate in COVID-19 pathogenesis through a process termed antibody-dependent enhancement. Rapid progress has been made in the research of antibody response and therapy in COVID-19 patients including characterization of the clinical features of antibody responses in different populations infected by SARS-CoV-2, treatment of COVID-19 patients with convalescent plasma and intravenous immunoglobin products, isolation and characterization of a large panel of monoclonal neutralizing antibodies, as well as preliminary clinical results from several COVID-19 vaccine candidates. In this review, we summarize the recent progress and discuss the implications of these findings in vaccine development.

Published
2020

10. Trav15-dv6 family Tcrd rearrangements diversify the Tcra repertoire

Author

Dauphars, Danielle J., Mihai, Ariana, Wang, Liuyang, Zhuang, Yuan, and Krangel, Michael S.

Abstract

The Tcra repertoire is generated by multiple rounds of Vα-Jα rearrangement. However, Tcrd recombination precedes Tcra recombination within the complex Tcra-Tcrd locus. Here, by ablating Tcrd recombination, we report that Tcrd rearrangement broadens primary Vα use to diversify the Tcra repertoire in mice. We reveal that use of Trav15-dv6 family V gene segments in Tcrd recombination imparts diversity in the Tcra repertoire by instigating use of central and distal Vα segments. Moreover, disruption of the regions containing these genes and their cis-regulatory elements identifies the Trav15-dv6 family as being responsible for driving central and distal Vα recombinations beyond their roles as substrates for Tcrd recombination. Our study demonstrates an indispensable role for Tcrd recombination in general, and the Trav15-dv6 family in particular, in the generation of a combinatorially diverse Tcra repertoire.

Published
2022
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11. Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.

Author

Dauphars DJ, Wu G, Bassing CH, and Krangel MS

Subjects
Mice, Animals, Polymerase Chain Reaction, Receptors, Antigen, T-Cell, alpha-beta genetics, Gene Rearrangement
Abstract

Quantitative real-time PCR and next-generation sequencing (NGS) are invaluable techniques to analyze T cell receptor (Tcr) gene rearrangements in mouse lymphocyte populations. Although these approaches are powerful, they also have limitations that must be accounted for in experimental design and data interpretation. Here, we provide relevant background required for understanding these limitations and then outline established quantitative real-time PCR and NGS methods that can be used for analysis of mouse Tcra and Tcrb gene rearrangements in mice., (© 2023. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.)

Published
2023
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