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2. Fecal microbiota and volatile metabolome pattern alterations precede late-onset meningitis in preterm neonates

Author

Frerichs, Nina M, primary, Deianova, Nancy, additional, el Manouni el Hassani, Sofia, additional, Acharjee, Animesh, additional, Quraishi, Mohammed Nabil, additional, de Boode, Willem P, additional, Cossey, Veerle, additional, Hulzebos, Christian V, additional, van Kaam, Anton H, additional, Kramer, Boris W, additional, d’Haens, Esther, additional, de Jonge, Wouter J, additional, Vijlbrief, Daniel C, additional, van Weissenbruch, Mirjam M, additional, Daulton, Emma, additional, Wicaksono, Alfian N, additional, Covington, James A, additional, Benninga, Marc A, additional, de Boer, Nanne K H, additional, van Goudoever, Johannes B, additional, Niemarkt, Hendrik J, additional, and de Meij, Tim G J, additional

Published
2024
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7. Biomimetic floating lipid membranes

Author

Daulton, Emma and Roser, Stephen

Subjects
540, lipid bilayer, Biomimetic membrane, neutron reflectometry, Langmuir monolayer
Published
2015

12. Discriminatory Ability of Gas Chromatography–Ion Mobility Spectrometry to Identify Patients Hospitalized With COVID-19 and Predict Prognosis

Author

Nazareth, Joshua, primary, Pan, Daniel, additional, Kim, Jee Whang, additional, Leach, Jack, additional, Brosnan, James G, additional, Ahmed, Adam, additional, Brodrick, Emma, additional, Bird, Paul, additional, Wicaksono, Alfian, additional, Daulton, Emma, additional, Tang, Julian W, additional, Williams, Caroline, additional, Haldar, Pranabashis, additional, Covington, James A, additional, Pareek, Manish, additional, and Sahota, Amandip, additional

Published
2022
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18. Editorial: volatile organic compound analysis to improve faecal immunochemical testing in the detection of colorectal cancer—Authors' reply

Author

Chandrapalan, Subashini, primary, Bosch, Sofie, additional, Tyagi, Heena, additional, Daulton, Emma, additional, Cubiella, Joaquín, additional, Guardiola, Jordi, additional, Kimani, Peter, additional, Mulder, Chris, additional, Covington, James, additional, Persaud, Krishna, additional, Meij, Tim G. J., additional, Altomare, Donato F., additional, Brenner, Herman, additional, Boer, Nanne K. H., additional, Ricciardiello, Luigi, additional, and Arasaradnam, Ramesh P., additional

Published
2021
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24. Faecal Volatile Organic Compounds to Detect Colorectal Neoplasia in Lynch Syndrome—A Prospective Longitudinal Multicentre Study.

Author

Liere, Elsa L. S. A., Ramsoekh, Dewkoemar, Daulton, Emma, Dakkak, Maya, Lingen, Joris M., Stewart, Trenton K., Bosch, Sofie, Carvalho, Beatriz, Dekker, Evelien, Jacobs, Maarten A. J. M., Koornstra, Jan Jacob, Kuijvenhoven, Johan P., Leerdam, Monique E., Meij, Tim G. J., Meijer, Gerrit A., Spaander, Manon C. W., Covington, James A., and Boer, Nanne K. H.

Subjects
*ION mobility spectroscopy, *HEREDITARY nonpolyposis colorectal cancer, *VOLATILE organic compounds, *COLORECTAL cancer, *OVERUSE injuries
Abstract

ABSTRACT Background Aim Methods Results Conclusions Trial Registration Non‐invasive biomarkers may reduce post‐colonoscopy colorectal cancer (CRC) rates and colonoscopy overuse in Lynch syndrome. Unlike faecal immunochemical test (FIT), faecal volatile organic compounds (VOCs) may accurately detect both advanced and non‐advanced colorectal neoplasia.The aim of this study was to evaluate the potential of faecal VOCs—separately and with FIT—to guide optimal colonoscopy intervals in Lynch syndrome.Prospective longitudinal multicentre study in which individuals with Lynch syndrome collected faeces before and after high‐quality surveillance colonoscopy. VOC‐patterns were analysed using field asymmetric ion mobility spectrometry (FAIMS) and gas chromatography‐ion mobility spectrometry (GC‐IMS) followed by machine learning pipelines, and combined with FIT at 2.55 μg Hb/g faeces. Gas chromatography time‐of‐flight mass spectrometry analysed individual VOC abundance.Among 200 included individuals (57% female, median 51 years), 62 had relevant neoplasia at colonoscopy: 3 CRC, 6 advanced adenoma (AA), 3 advanced serrated lesion (ASL), and 50 non‐advanced adenoma (NAA). Respective sensitivity and negative predictive value for CRC and AA (and also ASL in case of FAIMS) were 100% and 100% using FAIMS (54% specificity), and 89% and 99% using GC‐IMS (58% specificity). Respective sensitivity and specificity for any relevant neoplasia were 88% and 44% (FAIMS) and 84% and 28% (GC‐IMS); accuracy did not significantly improve upon VOC‐FIT. VOC‐patterns differed before and after polypectomy (AUC 0.70). NAA showed decreased faecal abundance of butanal, 2‐oxohexane, dimethyldisulphide and dimethyltrisulphide.In Lynch syndrome, faecal VOCs may be a promising strategy for postponing colonoscopy and for follow‐up after polypectomy. Our results serve as a stepping stone for large validation studies.NL8749 [ABSTRACT FROM AUTHOR]

Published
2024
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26. PTU-071 Risk stratification of symptomatic patients using faecal biomarkers and urinary volatile organic compounds

Author

Widlak, Monika, primary, Neal, Matthew, additional, Daulton, Emma, additional, Thomas, Claire, additional, Tomkins, Claudia, additional, Singh, Baljit, additional, Harmston, Chris, additional, Wicaksono, Alfian, additional, Evans, Charles, additional, Smith, Steve, additional, Savage, Richard, additional, Covington, James, additional, and Arasaradnam, Ramesh, additional

Published
2018
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27. An Accurate In Vitro Model of the E. coli Envelope

Author

Clifton, Luke A., Holt, Stephen A., Hughes, Arwel V., Daulton, Emma L., Arunmanee, Wanatchaporn, Heinrich, Frank, Khalid, Syma, Jefferies, Damien, Charlton, Timothy R., Webster, John R.P., Kinane, Christian J., and Lakey, Jeremy H.

Subjects
Models, Molecular, Gram‐negative Bakterien, Lipid Bilayers, FOS: Physical sciences, Antibiotika | Hot Paper, antibiotics, Zuschrift, drug discovery, Wirkstoff‐Forschung, Antibiotics Hot Paper, Drug Resistance, Bacterial, Escherichia coli, Humans, Struktur‐Aktivitäts‐Beziehungen, Escherichia coli Infections, Phospholipids, Escherichia coli Proteins, Membranen, Membranes, Artificial, General Medicine, Zuschriften, 20399 Classical Physics not elsewhere classified, Communications, Anti-Bacterial Agents, membranes, Gram-negative bacteria, structure–activity relationships, Antibiotika, Bacterial Outer Membrane Proteins
Abstract

Gram‐negative bacteria are an increasingly serious source of antibiotic‐resistant infections, partly owing to their characteristic protective envelope. This complex, 20 nm thick barrier includes a highly impermeable, asymmetric bilayer outer membrane (OM), which plays a pivotal role in resisting antibacterial chemotherapy. Nevertheless, the OM molecular structure and its dynamics are poorly understood because the structure is difficult to recreate or study in vitro. The successful formation and characterization of a fully asymmetric model envelope using Langmuir–Blodgett and Langmuir–Schaefer methods is now reported. Neutron reflectivity and isotopic labeling confirmed the expected structure and asymmetry and showed that experiments with antibacterial proteins reproduced published in vivo behavior. By closely recreating natural OM behavior, this model provides a much needed robust system for antibiotic development.

Published
2015
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28. High coverage fluid-phase floating lipid bilayers supported by ω-thiolipid self-assembled monolayers

Author

Hughes, Arwel V., Holt, Stephen A., Daulton, Emma, Soliakov, Andrei, Charlton, Timothy R., Roser, Steven J., and Lakey, Jeremy H.

Subjects
thiolipid, Surface Properties, Lipid Bilayers, Water, Bayes Theorem, gold, Surface Plasmon Resonance, neutron reflection, Lipids, Membrane Potentials, Magnetics, self-assembled monolayer, floating supported bilayer, magnetic contrast, Phosphatidylcholines, Sulfhydryl Compounds, Electrodes, Research Articles, Phospholipids
Abstract

Large area lipid bilayers, on solid surfaces, are useful in physical studies of biological membranes. It is advantageous to minimize the interactions of these bilayers with the substrate and this can be achieved via the formation of a floating supported bilayer (FSB) upon either a surface bound phospholipid bilayer or monolayer. The FSB's independence is enabled by the continuous water layer (greater than 15 Å) that remains between the two. However, previous FSBs have had limited stability and low density. Here, we demonstrate by surface plasmon resonance and neutron reflectivity, the formation of a complete self-assembled monolayer (SAM) on gold surfaces by a synthetic phosphatidylcholine bearing a thiol group at the end of one fatty acyl chain. Furthermore, a very dense FSB (more than 96%) of saturated phosphatidylcholine can be formed on this SAM by sequential Langmuir-Blodgett and Langmuir-Schaefer procedures. Neutron reflectivity used both isotopic and magnetic contrast to enhance the accuracy of the data fits. This system offers the means to study transmembrane proteins, membrane potential effects (using the gold as an electrode) and even model bacterial outer membranes. Using unsaturated phosphatidylcholines, which have previously failed to form stable FSBs, we achieved a coverage of 73%.

Published
2014

29. An Accurate In Vitro Model of theE. coliEnvelope

Author

Clifton, Luke A., primary, Holt, Stephen A., additional, Hughes, Arwel V., additional, Daulton, Emma L., additional, Arunmanee, Wanatchaporn, additional, Heinrich, Frank, additional, Khalid, Syma, additional, Jefferies, Damien, additional, Charlton, Timothy R., additional, Webster, John R. P., additional, Kinane, Christian J., additional, and Lakey, Jeremy H., additional

Published
2015
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30. An Accurate In Vitro Model of the E. coli Envelope

Author

Clifton, Luke A., primary, Holt, Stephen A., additional, Hughes, Arwel V., additional, Daulton, Emma L., additional, Arunmanee, Wanatchaporn, additional, Heinrich, Frank, additional, Khalid, Syma, additional, Jefferies, Damien, additional, Charlton, Timothy R., additional, Webster, John R. P., additional, Kinane, Christian J., additional, and Lakey, Jeremy H., additional

Published
2015
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33. Students' Voice.

Author

Southall, Caroline, Douglas, Sarah, Daulton, Emma, and Horan, P. A.

Published
1980

34. Faecal Volatile Organic Compounds to Detect Colorectal Neoplasia in Lynch Syndrome-A Prospective Longitudinal Multicentre Study.

Author

van Liere ELSA, Ramsoekh D, Daulton E, Dakkak M, van Lingen JM, Stewart TK, Bosch S, Carvalho B, Dekker E, Jacobs MAJM, Koornstra JJ, Kuijvenhoven JP, van Leerdam ME, de Meij TGJ, Meijer GA, Spaander MCW, Covington JA, and de Boer NKH

Abstract

Background: Non-invasive biomarkers may reduce post-colonoscopy colorectal cancer (CRC) rates and colonoscopy overuse in Lynch syndrome. Unlike faecal immunochemical test (FIT), faecal volatile organic compounds (VOCs) may accurately detect both advanced and non-advanced colorectal neoplasia., Aim: The aim of this study was to evaluate the potential of faecal VOCs-separately and with FIT-to guide optimal colonoscopy intervals in Lynch syndrome., Methods: Prospective longitudinal multicentre study in which individuals with Lynch syndrome collected faeces before and after high-quality surveillance colonoscopy. VOC-patterns were analysed using field asymmetric ion mobility spectrometry (FAIMS) and gas chromatography-ion mobility spectrometry (GC-IMS) followed by machine learning pipelines, and combined with FIT at 2.55 μg Hb/g faeces. Gas chromatography time-of-flight mass spectrometry analysed individual VOC abundance., Results: Among 200 included individuals (57% female, median 51 years), 62 had relevant neoplasia at colonoscopy: 3 CRC, 6 advanced adenoma (AA), 3 advanced serrated lesion (ASL), and 50 non-advanced adenoma (NAA). Respective sensitivity and negative predictive value for CRC and AA (and also ASL in case of FAIMS) were 100% and 100% using FAIMS (54% specificity), and 89% and 99% using GC-IMS (58% specificity). Respective sensitivity and specificity for any relevant neoplasia were 88% and 44% (FAIMS) and 84% and 28% (GC-IMS); accuracy did not significantly improve upon VOC-FIT. VOC-patterns differed before and after polypectomy (AUC 0.70). NAA showed decreased faecal abundance of butanal, 2-oxohexane, dimethyldisulphide and dimethyltrisulphide., Conclusions: In Lynch syndrome, faecal VOCs may be a promising strategy for postponing colonoscopy and for follow-up after polypectomy. Our results serve as a stepping stone for large validation studies., Trial Registration: NL8749., (© 2024 The Author(s). Alimentary Pharmacology & Therapeutics published by John Wiley & Sons Ltd.)

Published
2024
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35. An Accurate In Vitro Model of the E. coli Envelope.

Author

Clifton LA, Holt SA, Hughes AV, Daulton EL, Arunmanee W, Heinrich F, Khalid S, Jefferies D, Charlton TR, Webster JR, Kinane CJ, and Lakey JH

Abstract

Gram-negative bacteria are an increasingly serious source of antibiotic-resistant infections, partly owing to their characteristic protective envelope. This complex, 20 nm thick barrier includes a highly impermeable, asymmetric bilayer outer membrane (OM), which plays a pivotal role in resisting antibacterial chemotherapy. Nevertheless, the OM molecular structure and its dynamics are poorly understood because the structure is difficult to recreate or study in vitro. The successful formation and characterization of a fully asymmetric model envelope using Langmuir-Blodgett and Langmuir-Schaefer methods is now reported. Neutron reflectivity and isotopic labeling confirmed the expected structure and asymmetry and showed that experiments with antibacterial proteins reproduced published in vivo behavior. By closely recreating natural OM behavior, this model provides a much needed robust system for antibiotic development.

Published
2015
Full Text
View/download PDF

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