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Your search keyword '"Dassie, Justin P."' showing total 16 results
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16 results on '"Dassie, Justin P."'

1. Combination of protein and cell internalization SELEX identifies a potential RNA therapeutic and delivery platform to treat EphA2-expressing tumors

Author

Santana-Viera, Laura, Dassie, Justin P., Rosàs-Lapeña, Marta, Garcia-Monclús, Silvia, Chicón-Bosch, Mariona, Pérez-Capó, Marina, Pozo, Lidia del, Sanchez-Serra, Sara, Almacellas-Rabaiget, Olga, Maqueda-Marcos, Susana, López-Alemany, Roser, Thiel, William H., Giangrande, Paloma H., and Tirado, Oscar M.

Published
2023
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2. Monitoring of breast cancer progression via aptamer-based detection of circulating tumor cells in clinical blood samples

Author

Kolovskaya, Olga S., primary, Zyuzyukina, Alena V., additional, Dassie, Justin P., additional, Zamay, Galina S., additional, Zamay, Tatiana N., additional, Boyakova, Nina V., additional, Khorzhevskii, Vladimir A., additional, Kirichenko, Daria A., additional, Lapin, Ivan N., additional, Shchugoreva, Irina A., additional, Artyushenko, Polina V., additional, Tomilin, Felix N., additional, Veprintsev, Dmitry V., additional, Glazyrin, Yury E., additional, Minic, Zoran, additional, Bozhenko, Vladimir K., additional, Kudinova, Elena A., additional, Kiseleva, Yana Y., additional, Krat, Alexey V., additional, Slepov, Eugene V., additional, Bukatin, Anton S., additional, Zukov, Ruslan A., additional, Shesternya, Pavel A., additional, Berezovski, Maxim V., additional, Giangrande, Paloma H., additional, and Kichkailo, Anna S., additional

Published
2023
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10. 61. Vascular Smooth Muscle Cell RNA Aptamers for the Treatment of Cardiovascular Disease

Author

Thiel, William H., primary, Esposito, Carla L., additional, Dickey, David D., additional, Dassie, Justin P., additional, Long, Matthew E., additional, Adam, Joshua, additional, Streeter, Jennifer, additional, Schickling, Brandon, additional, Takapoo, Maysam, additional, Flenker, Katie S., additional, Klesney-Tait, Julia, additional, de Franciscis, Vittorio, additional, Miller, Francis J., additional, and Giangrande, Paloma H., additional

Published
2015
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11. Targeted Inhibition of Prostate Cancer Metastases with an RNA Aptamer to Prostate-specific Membrane Antigen

Author

Dassie, Justin P, primary, Hernandez, Luiza I, additional, Thomas, Gregory S, additional, Long, Matthew E, additional, Rockey, William M, additional, Howell, Craig A, additional, Chen, Yani, additional, Hernandez, Frank J, additional, Liu, Xiu Ying, additional, Wilson, Mary E, additional, Allen, Lee-Ann, additional, Vaena, Daniel A, additional, Meyerholz, David K, additional, and Giangrande, Paloma H, additional

Published
2014
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12. Multifunctional Aptamer-miRNA Conjugates for Targeted Cancer Therapy

Author

Esposito, Carla L, primary, Cerchia, Laura, additional, Catuogno, Silvia, additional, De Vita, Gennaro, additional, Dassie, Justin P, additional, Santamaria, Gianluca, additional, Swiderski, Piotr, additional, Condorelli, Gerolama, additional, Giangrande, Paloma H, additional, and de Franciscis, Vittorio, additional

Published
2014
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13. Nucleotide Bias Observed with a Short SELEX RNA Aptamer Library

Author

Thiel, William H., primary, Bair, Thomas, additional, Wyatt Thiel, Kristina, additional, Dassie, Justin P., additional, Rockey, William M., additional, Howell, Craig A., additional, Liu, Xiuying Y., additional, Dupuy, Adam J., additional, Huang, Lingyan, additional, Owczarzy, Richard, additional, Behlke, Mark A., additional, McNamara, James O., additional, and Giangrande, Paloma H., additional

Published
2011
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15. Rapid “Open-Source” Engineering of Customized Zinc-Finger Nucleases for Highly Efficient Gene Modification

Author

Maeder, Morgan L., primary, Thibodeau-Beganny, Stacey, additional, Osiak, Anna, additional, Wright, David A., additional, Anthony, Reshma M., additional, Eichtinger, Magdalena, additional, Jiang, Tao, additional, Foley, Jonathan E., additional, Winfrey, Ronnie J., additional, Townsend, Jeffrey A., additional, Unger-Wallace, Erica, additional, Sander, Jeffry D., additional, Müller-Lerch, Felix, additional, Fu, Fengli, additional, Pearlberg, Joseph, additional, Göbel, Carl, additional, Dassie, Justin P., additional, Pruett-Miller, Shondra M., additional, Porteus, Matthew H., additional, Sgroi, Dennis C., additional, Iafrate, A. John, additional, Dobbs, Drena, additional, McCray, Paul B., additional, Cathomen, Toni, additional, Voytas, Daniel F., additional, and Joung, J. Keith, additional

Published
2008
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16. Method for Confirming Cytoplasmic Delivery of RNA Aptamers.

Author

Dickey DD, Thomas GS, Dassie JP, and Giangrande PH

Subjects
Antigens, Surface metabolism, Aptamers, Nucleotide genetics, Cell Line, Drug Carriers toxicity, Glutamate Carboxypeptidase II metabolism, Humans, RNA Interference, Ribosome Inactivating Proteins, Type 1 toxicity, Saporins, Aptamers, Nucleotide metabolism, Cytoplasm metabolism, Drug Carriers metabolism, Ribosome Inactivating Proteins, Type 1 metabolism
Abstract

RNA aptamers are single-stranded RNA oligos that represent a powerful emerging technology with potential for treating numerous diseases. More recently, cell-targeted RNA aptamers have been developed for delivering RNA interference (RNAi) modulators (siRNAs and miRNAs) to specific diseased cells (e.g., cancer cells or HIV infected cells) in vitro and in vivo. However, despite initial promising reports, the broad application of this aptamer delivery technology awaits the development of methods that can verify and confirm delivery of aptamers to the cytoplasm of target cells where the RNAi machinery resides. We recently developed a functional assay (RIP assay) to confirm cellular uptake and subsequent cytoplasmic release of an RNA aptamer which binds to a cell surface receptor expressed on prostate cancer cells (PSMA). To assess cytoplasmic delivery, the aptamer was chemically conjugated to saporin, a ribosome inactivating protein toxin that is toxic to cells only when delivered to the cytoplasm (where it inhibits the ribosome) by a cell-targeting ligand (e.g., aptamer). Here, we describe the chemistry used to conjugate the aptamer to saporin and discuss a gel-based method to verify conjugation efficiency. We also detail an in vitro functional assay to confirm that the aptamer retains function following conjugation to saporin and describe a cellular assay to measure aptamer-mediated saporin-induced cytotoxicity.

Published
2016
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