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Background/aim: Natural products are popular insights for researchers to investigate promising anti-cancer agents since some of these substances have lesser adverse effects restricting the treatment than traditional chemotherapeutic agents. A well-known monoterpene Carvacrol, widely consumed in Mediterranean cuisine and lower risks of cancer, has efficient anticancer effects. However, the mechanism of action is yet to be discovered. Materials and methods: The investigation aims to illuminate a new perceptive in the role of this substance on colorectal cancer treatment, by the means of differences in a well-defined range of soluble factors. Carvacrol effect on both HT-29 and HCT-116 cell lines was evaluated on proliferation and the IC50 values were calculated by the RTCA xCELLigence device. Then MAGPIX assay was performed to obtain the changes in soluble factors of the cell lines. Results: The Multiplexing assay suggests some of these factors were altered in favor of surviving and proliferation in aggressive cell line HCT-116 whereas they were altered against these characters in HT-29, were correlated with the increased IC50 concentration of HCT-116 in carvacrol treatment. Conclusion: The current study indicates that differences in the levels of these soluble factors could modulate the anticancer effect related to carvacrol. [ABSTRACT FROM AUTHOR]

OBJECTIVES: Recently, the development of technologies to culture intestinal epithelial cells in vitro as different forms of intestinal organoids causes drawing attention in this area. The isolation and culture of colorectal crypts were first demonstrated in the very recent decade with the identification of stem cell marker Lgr5. The growth of crypts into organoids provides an in vitro model for studying the mucosal physiology, colorectal cancer tumorigenesis, and regeneration. In the current study, cancer stem cell isolation, tissue decontamination, organoid freezing and thawing processes, and organoid culture media ingredients have been evaluated. The aim of this investigation is optimization and production of human colorectal cancer organoids. MATERIAL & METHODS: The colorectal cancerous tissues are collected from Dokuz Eylul University Hospital. Isolation method of cancer cell is performed by EDTA solution and agitation is provided by shaker. Matrigel is used to create optimal 3D structure to grow cancer stem cells up to colorectal tissue. L-WRN cell line has been used to produce secreted factors which are WNT-3A, Rspondin and Noggin in purpose of supporting the development of organoids. RESULTS: Different parameters have been evaluated to obtain more efficient organoids. The experiment is performed in our laboratory presented that, human colorectal cancer organoids have been generated and manipulated in our laboratory. CONCLUSIONS: The currency protocol explains how the generation of 3D organoid cultures from cancerous human colorectal tissue is manipulated. The in vitro 3D organoid model is a promising and novel approach which present us more real-like working material. [ABSTRACT FROM AUTHOR]