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1. Quality of life following maxillofacial trauma in the elderly: a multicenter, prospective study

Author

Boffano, Paolo, Pau, Alberto, Dosio, Camilla, Ruslin, Muhammad, Forouzanfar, Tymour, Rodríguez-Santamarta, Tanía, de Vicente, Juan Carlos, Tarle, Marko, Dediol, Emil, Pechalova, Petia, Pavlov, Nikolai, Daskalov, Hristo, Doykova, Iva, Kelemith, Kadri, Tamme, Tiia, Kopchak, Andrey, Yu, Romanova Anna, Corre, Pierre, Bertin, Helios, Bourry, Maeva, Guyonvarc’h, Pierre, Jezdić, Zoran, Konstantinovic, Vitomir S., Starch-Jensen, Thomas, and Brucoli, Matteo

Published
2022
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3. The epidemiology of edentulous atrophic mandibular fractures in Europe

Author

Brucoli, Matteo, Boffano, Paolo, Romeo, Irene, Corio, Chiara, Benech, Arnaldo, Ruslin, Muhammad, Forouzanfar, Tymour, Rodríguez-Santamarta, Tanía, de Vicente, Juan Carlos, Tarle, Marko, Dediol, Emil, Pechalova, Petia, Pavlov, Nikolai, Daskalov, Hristo, Doykova, Iva, Kelemith, Kadri, Tamme, Tiia, Kopchak, Andrey, Shumynskyi, Ievgen, Corre, Pierre, Bertin, Helios, Bourry, Maeva, Guyonvarc'h, Pierre, Dovšak, Tadej, Vozlič, David, Birk, Anže, Aničić, Boban, Konstantinovic, Vitomir S., and Starch-Jensen, Thomas

Published
2019
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4. Surgical management of unilateral body fractures of the edentulous atrophic mandible

Author

Brucoli, Matteo, Boffano, Paolo, Romeo, Irene, Corio, Chiara, Benech, Arnaldo, Ruslin, Muhammad, Forouzanfar, Tymour, Rodríguez-Santamarta, Tanía, Vicente, Juan Carlos de, Tarle, Marko, Dediol, Emil, Pechalova, Petia, Pavlov, Nikolai, Daskalov, Hristo, Doykova, Iva, Kelemith, Kadri, Tamme, Tiia, Kopchak, Andrey, Shumynskyi, Ievgen, Corre, Pierre, Bertin, Helios, Bourry, Maeva, Guyonvarc’h, Pierre, Dovšak, Tadej, Vozlič, David, Birk, Anže, Aničić, Boban, Konstantinovic, Vitomir S., and Starch-Jensen, Thomas

Published
2020
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5. DETECTION AND ANTIMICROBIAL RESISTANCE OF COAGULASE-POSITIVE STAPHYLOCOCCUS AUREUS AND METHICILIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM RAW PORK IN THE RETAIL.

Author

Krumova-Valcheva, Gergana, Mateva, Gergana, Milanov, Mihail, Gyurova, Eva, and Daskalov, Hristo

Subjects
ANTI-infective agents, STAPHYLOCOCCUS aureus, MULTIDRUG resistance, CEFOXITIN
Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is widespread in pork meat and can cause a severe food poisoning. The aim of present study is to determine the prevalence and characteristics of the antimicrobial resistance of S. aureus and MRSA from retail raw pork meat in Bulgaria between 2019 and 2021. Coagulase-positive S. aureus were detected in 30.8% (52/169). Susceptibility to all antimicrobials was detected in 28.8% (15/52) of S. aureus isolates by the disk-diffusion method. Multidrug resistance was found in 18 of a total of 52 isolates (34.6%). A study of the MIC indicated resistance to the cefoxitin (> 4 mg/L) in 8 of the total 23 tested isolates (34.8%) The mecA gene was confirmed in 5 of the tested isolates. The high prevalence of MRSA in raw pork meat, sold in Bulgaria should be considered as a signal of the risk of its spread in the human population. This requires the implementation of appropriate hygiene practices in the production, processing and sale of meat and meat products to reduce the spread of MRSA to humans. [ABSTRACT FROM AUTHOR]

Published
2024

8. Assessing pig farm biosecurity measures for the control of Salmonella on European farms

Author

Smith, Richard P., primary, May, Hannah E., additional, Burow, Elke, additional, Meester, Marina, additional, Tobias, Tijs J., additional, Sassu, Elena-Lucia, additional, Pavoni, Enrico, additional, Di Bartolo, Ilaria, additional, Prigge, Christopher, additional, Wasyl, Dariusz, additional, Zmudzki, Jacek, additional, Viltrop, Arvo, additional, Nurmoja, Imbi, additional, Zoche-Golob, Veit, additional, Alborali, Giovanni L., additional, Romantini, Romina, additional, Dors, Arkadiusz, additional, Krumova-Valcheva, Gergana, additional, Koláčková, Ivana, additional, Aprea, Guiseppe, additional, and Daskalov, Hristo, additional

Published
2023
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11. Study on factors (pH, water activity, salt content) affecting the growth of Listeria Monocytogenes in raw dried cured sausages

Author

Daskalov Hristo, Fejzullah Fejzulla, and Stoyahchev Todora

Subjects
L.monocytogenes, raw dried cured sausage, water activity, pH, salt, Veterinary medicine, SF600-1100
Abstract

Commission Regulation (EC) 2073:2005 considers the factors which can support or inhibit the growth of Listeria monocytogenesin ready-to-eat foods. The aim of the experiments was to examine the values of pH, water activity (aw), salt content and level of contamination with Listeria monocytogenes of some popular in Bulgaria raw dried cured vacuum packed sausages, produced from June 2006 till May 2008. 81 vacuum-packed samples were taken from 5 different meat producing plants during the period of study. Average water activity level of the tested sausages was 0,87 ± 0,035; pH level - 5,61 ± 0,59 and salt content - 4,12 ± 1,11%. Four specimens contained Listeria spp. (two samples L.monocytogenes, one L.welshimeri and one L.innocua). All contaminated raw dried cured sausages had aw bellow ≤ 0,92 and pH ≥ 4,4 or pH ≥ 5. After 3 months of storage of the same contaminated samples at 40C, in three of them Listeria spp. (two L.monocitogenes and L.welshimeri) survived and was detected. Salt content of the samples varied from 2,46 to 6,28% and was not able to affect the growth of L.monocytogenes.Data showed that the detected levels of aw could support the growth of L.monocytogenes in only 6 (7,4%) of the tested samples. pH values lower than 5 were presented in three samples and only the combination with low aw was able to inhibit the growth of L.monocytogenes. The detected levels of salt content did not affect the presence and growth of L.monocytogenes. ‘Microbiological criterion’ set in COMMISSION REGULATION (EC) No 2073/2005 for ready-to-eat foods unable to support the growth of L. monocytogenescan be applied to 75 (92,6%) of the tested sausages.

Published
2013

12. Genomic evolution of antimicrobial resistance in Escherichia coli

Author

Leekitcharoenphon, Pimlapas, Johansson, Markus Hans Kristofer, Munk, Patrick, Malorny, Burkhard, Skarzynska, Magdalena, Wadepohl, Katharina, Moyano, Gabriel, Hesp, Ayla, Veldman, Kees T., Bossers, Alex, Graveland, Haitske, van Essen, Alieda, Battisti, Antonio, Caprioli, Andrea, Blaha, Thomas, Hald, Tine, Daskalov, Hristo, Saatkamp, Helmut W., Staerk, Katharina D. C., Luiken, Roosmarijn E. C., Van Gompel, Liese, Hansen, Rasmus Borup, Dewulf, Jeroen, Duarte, Ana Sofia Ribeiro, Zajac, Magdalena, Wasyl, Dariusz, Sanders, Pascal, Gonzalez-Zorn, Bruno, Brouwer, Michael S. M., Wagenaar, Jaap A., Heederik, Dick J. J., Mevius, Dik, Aarestrup, Frank M., EFFORT Consortium, for the, Technical University of Denmark [Lyngby] (DTU), Bundesinstitut für Risikobewertung - Federal Institute for Risk Assessment (BfR), Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Wageningen BioVeterinary Research, Wageningen University and Research [Wageningen] (WUR), Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), European Project: 613754,EC:FP7:KBBE,FP7-KBBE-2013-7-single-stage,EFFORT(2013), Danmarks Tekniske Universitet = Technical University of Denmark (DTU), IRAS OH Epidemiology Microbial Agents, Klinische infectiologie en microb. lab., dI&I I&I-4, dIRAS RA-I&I RA, and Faculteit Diergeneeskunde

Subjects
0301 basic medicine, Genomic alteration, antibiotic resistance, Swine, Epidemiology, résistance aux antibiotiques, veterinary drug, medicine.disease_cause, Genome, Poultry, Feces, animal, évolution, bacteria, Escherichia coli Infections, Phylogeny, médicament humainélevage, 2. Zero hunger, Genetics, human drug, bactérie, Multidisciplinary, Virulence, Bacteriologie, transmission, Genomics, Bacteriology, Host Pathogen Interaction & Diagnostics, Text, Anti-Bacterial Agents, Europe, Medicine, médicament, médicament vétérinaire, Bioinformatica & Diermodellen, Science, 030106 microbiology, Microbial Sensitivity Tests, GENETIC-STRUCTURE, Biology, Article, Evolution, Molecular, 03 medical and health sciences, Antibiotic resistance, résistance aux antimicrobiens, Drug Resistance, Bacterial, Bio-informatics & Animal models, medicine, Escherichia coli, Animals, Life Science, Epidemiology, Bio-informatics & Animal models, ddc:610, Veterinary Sciences, antimicrobial resistance, microbiologie, General, genome, Host Pathogen Interaction & Diagnostics, Epidemiologie, Whole genome sequencing, génome, microbiology, Bacteriology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Host Pathogen Interactie & Diagnostiek, livestock, Red Meat, 030104 developmental biology, Metagenomics, Genetic marker, Epidemiologie, Bioinformatica & Diermodellen, Bacteriologie, Host Pathogen Interactie & Diagnostiek, Molecular evolution, Cattle, POULTRY, Mobile genetic elements, humain, Genome, Bacterial
Abstract

The emergence of antimicrobial resistance (AMR) is one of the biggest health threats globally. In addition, the use of antimicrobial drugs in humans and livestock is considered an important driver of antimicrobial resistance. The commensal microbiota, and especially the intestinal microbiota, has been shown to have an important role in the emergence of AMR. Mobile genetic elements (MGEs) also play a central role in facilitating the acquisition and spread of AMR genes. We isolated Escherichia coli (n = 627) from fecal samples in respectively 25 poultry, 28 swine, and 15 veal calf herds from 6 European countries to investigate the phylogeny of E. coli at country, animal host and farm levels. Furthermore, we examine the evolution of AMR in E. coli genomes including an association with virulence genes, plasmids and MGEs. We compared the abundance metrics retrieved from metagenomic sequencing and whole genome sequenced of E. coli isolates from the same fecal samples and farms. The E. coli isolates in this study indicated no clonality or clustering based on country of origin and genetic markers; AMR, and MGEs. Nonetheless, mobile genetic elements play a role in the acquisition of AMR and virulence genes. Additionally, an abundance of AMR was agreeable between metagenomic and whole genome sequencing analysis for several AMR classes in poultry fecal samples suggesting that metagenomics could be used as an indicator for surveillance of AMR in E. coli isolates and vice versa.

Published
2021

14. Antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) abundance in animal faeces, meat, production environments and human faeces in Europe

Author

Yang, Dongsheng, Heederik, Dick J.J., Scherpenisse, Peter, Van Gompel, Liese, Luiken, Roosmarijn E.C., Wadepohl, Katharina, Skarżyńska, Magdalena, Van Heijnsbergen, Eri, Wouters, Inge M., Greve, Gerdit D., Jongerius-Gortemaker, Betty G.M., Tersteeg-Zijderveld, Monique, Portengen, Lützen, Juraschek, Katharina, Fischer, Jennie, Zajac, Magdalena, Wasyl, Dariusz, Wagenaar, Jaap A., Mevius, Dik J., Smit, Lidwien A.M., Schmitt, Heike, Graveland, Haitske, Joosten, Philip, Sarrazin, Steven, Dewulf, Jeroen, Van Essen, Alieda, Gonzalez-Zorn, Bruno, Moyano, Gabriel, Sanders, Pascal, David, Julie, Soumet, Christophe, Battisti, Antonio, Caprioli, Andrea, Blaha, Thomas, Brandt, Maximiliane, Aarestrup, Frank, Hald, Tine, Duarte, Ana Sofia Ribeiro, Hoszowski, Andrzej, Pekala-Safinnska, Agnieszka, Pazdzior, Ewa, Daskalov, Hristo, Saatkamp, Helmut W., Stark, Katharina D.C., IRAS OH Epidemiology Microbial Agents, Faculteit Diergeneeskunde, dIRAS RA-I&I RA, dIRAS RA-I&I I&I, Klinische infectiologie en microb. lab., LS IRAS EEPI GRA (Gezh.risico-analyse), One Health Microbieel, IRAS OH Epidemiology Chemical Agents, dIRAS RA-2, and dI&I I&I-4

Subjects
Microbiology (medical), Livestock, Meat, Drivers, Swine, Epidemiology, Bioinformatica & Diermodellen, GTB Gewasgez. Bodem en Water, Bedrijfseconomie, WASS, Escherichia-coli, Feces, Crop health, Anti-Infective Agents, Business Economics, RNA, Ribosomal, 16S, Drug Resistance, Bacterial, Bio-informatics & Animal models, Mechanisms, Animals, Humans, Life Science, Epidemiology, Bio-informatics & Animal models, Pharmacology (medical), Host Pathogen Interaction & Diagnostics, Epidemiologie, Pharmacology, Carriage, Ecology, Bacteriologie, Bacteriology, Bacteriology, Host Pathogen Interaction & Diagnostics, Chicken, Host Pathogen Interactie & Diagnostiek, Anti-Bacterial Agents, Risk-factors, Cross-Sectional Studies, Infectious Diseases, Genes, Bacterial, Epidemiologie, Bioinformatica & Diermodellen, Gewasgezondheid, Bacteriologie, Host Pathogen Interactie & Diagnostiek, Cattle, Pigs, Chickens
Abstract

Background Real-time quantitative PCR (qPCR) is an affordable method to quantify antimicrobial resistance gene (ARG) targets, allowing comparisons of ARG abundance along animal production chains. Objectives We present a comparison of ARG abundance across various animal species, production environments and humans in Europe. AMR variation sources were quantified. The correlation of ARG abundance between qPCR data and previously published metagenomic data was assessed. Methods A cross-sectional study was conducted in nine European countries, comprising 9572 samples. qPCR was used to quantify abundance of ARGs [aph(3′)-III, erm(B), sul2, tet(W)] and 16S rRNA. Variance component analysis was conducted to explore AMR variation sources. Spearman’s rank correlation of ARG abundance values was evaluated between pooled qPCR data and earlier published pooled metagenomic data. Results ARG abundance varied strongly among animal species, environments and humans. This variation was dominated by between-farm variation (pigs) or within-farm variation (broilers, veal calves and turkeys). A decrease in ARG abundance along pig and broiler production chains (‘farm to fork’) was observed. ARG abundance was higher in farmers than in slaughterhouse workers, and lowest in control subjects. ARG abundance showed a high correlation (Spearman’s ρ > 0.7) between qPCR data and metagenomic data of pooled samples. Conclusions qPCR analysis is a valuable tool to assess ARG abundance in a large collection of livestock-associated samples. The between-country and between-farm variation of ARG abundance could partially be explained by antimicrobial use and farm biosecurity levels. ARG abundance in human faeces was related to livestock antimicrobial resistance exposure.

Published
2022

15. Quality of life following maxillofacial trauma in the elderly: a multicenter, prospective study

Author

Boffano, Paolo, primary, Pau, Alberto, additional, Dosio, Camilla, additional, Ruslin, Muhammad, additional, Forouzanfar, Tymour, additional, Rodríguez-Santamarta, Tanía, additional, de Vicente, Juan Carlos, additional, Tarle, Marko, additional, Dediol, Emil, additional, Pechalova, Petia, additional, Pavlov, Nikolai, additional, Daskalov, Hristo, additional, Doykova, Iva, additional, Kelemith, Kadri, additional, Tamme, Tiia, additional, Kopchak, Andrey, additional, Yu, Romanova Anna, additional, Corre, Pierre, additional, Bertin, Helios, additional, Bourry, Maeva, additional, Guyonvarc’h, Pierre, additional, Jezdić, Zoran, additional, Konstantinovic, Vitomir S., additional, Starch-Jensen, Thomas, additional, and Brucoli, Matteo, additional

Published
2021
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16. Management of mandibular condylar fractures in patients with atrophic edentulous mandibles

Author

Brucoli, Matteo, Boffano, Paolo, Romeo, Irene, Corio, Chiara, Benech, Arnaldo, Ruslin, Muhammad, Forouzanfar, Tymour, Starch‐Jensen, Thomas, Rodríguez‐ Santamarta, Tanía, de Vicente, Juan Carlos, Tarle, Marko, Dediol, Emil, Pechalova, Petia, Pavlov, Nikolai, Daskalov, Hristo, Doykova, Iva, Kelemith, Kadri, Tamme, Tiia, Kopchak, Andrey, Shumynskyi, Ievgen, Corre, Pierre, Bertin, Helios, Bourry, Maeva, Guyonvarc’h, Pierre, Dovšak, Tadej, Vozlič, David, Birk, Anže, Aničić, Boban, Konstantinovic, Vitomir S., Starch-Jensen, Thomas, Oral and Maxillofacial Surgery / Oral Pathology, AMS - Tissue Function & Regeneration, and Maxillofacial Surgery (VUmc)

Subjects
Patient Consent, medicine.medical_treatment, Mandible, Atrophic mandible, Condylar fracture, Edentulous, Management, Surgery, Condyle, 03 medical and health sciences, Fracture Fixation, Internal, 0302 clinical medicine, stomatognathic system, atrophic mandible, condylar fracture, edentulous, management, surgery, Mandibular Fractures, medicine, Internal fixation, Humans, In patient, 030223 otorhinolaryngology, 10. No inequality, Reduction (orthopedic surgery), Orthodontics, business.industry, Mandibular Condyle, 030206 dentistry, SDG 10 - Reduced Inequalities, 3. Good health, Europe, Otorhinolaryngology, Oral and maxillofacial surgery, Oral Surgery, business, Edentulous patient
Abstract

INTRODUCTION: Treatment of condylar fractures in patients with atrophic edentulous mandibles is a peculiar field that has been little considered in the literature. The aim of the study was to assess the demographic and clinical variables as well as management and outcome of mandibular condylar fractures in edentulous patients with atrophic mandibles that were treated at several European departments of oral and maxillofacial surgery.METHODS: The data of all patients with fractures of the atrophic edentulous mandible from the involved maxillofacial surgical units across Europe between January 1, 2008, and December 31, 2017. Only patients that were diagnosed with condylar fractures of the edentulous atrophic mandible were included.RESULTS: A total of 52 patients met the inclusion criteria and were included in the study: 79% of patients reported one or more comorbidities. Thirty-four unilateral neck or subcondylar fractures, 9 bilateral neck or subcondylar condylar fractures, 7 unilateral head condylar fractures, and 2 bilateral head condylar fractures were diagnosed. No treatment was performed in 37 cases, whereas in 4 patients a closed treatment was decided, and 11 patients underwent open reduction and internal fixation. Outcome was considered to be satisfying in 48 patients, with no complications.CONCLUSIONS: The golden rule still remains that the diagnosis of a subcondylar or neck fracture in an edentulous patient should constitute an indication for open reduction and internal fixation. However, an appropriate choice of management options has to be individualized on a case by case basis, also depending on the patient consent.

Published
2020

18. NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Staphylococcus aureus Strains Responsible for FoodBorne Outbreaks

Author

Merda, Déborah, primary, Felten, Arnaud, additional, Vingadassalon, Noémie, additional, Denayer, Sarah, additional, Titouche, Yacine, additional, Decastelli, Lucia, additional, Hickey, Bernadette, additional, Kourtis, Christos, additional, Daskalov, Hristo, additional, Mistou, Michel-Yves, additional, and Hennekinne, Jacques-Antoine, additional

Published
2020
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19. Management of maxillofacial trauma in the elderly: A European multicenter study

Author

Brucoli, Matteo, primary, Boffano, Paolo, additional, Romeo, Irene, additional, Corio, Chiara, additional, Benech, Arnaldo, additional, Ruslin, Muhammad, additional, Forouzanfar, Tymour, additional, Starch‐Jensen, Thomas, additional, Rodríguez‐Santamarta, Tanía, additional, de Vicente, Juan Carlos, additional, Snäll, Johanna, additional, Thorén, Hanna, additional, Tarle, Marko, additional, Dediol, Emil, additional, Pechalova, Petia, additional, Pavlov, Nikolai, additional, Daskalov, Hristo, additional, Doykova, Iva, additional, Kelemith, Kadri, additional, Tamme, Tiia, additional, Kopchak, Andrey, additional, Shumynskyi, Ievgen, additional, Corre, Pierre, additional, Bertin, Helios, additional, Goguet, Quentin, additional, Anquetil, Marine, additional, Louvrier, Aurélien, additional, Meyer, Christophe, additional, Dovšak, Tadej, additional, Vozlič, David, additional, Birk, Anže, additional, Aničić, Boban, additional, and Konstantinovic, Vitomir S., additional

Published
2020
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20. Surgical management of unilateral body fractures of the edentulous atrophic mandible

Author

Brucoli, Matteo, primary, Boffano, Paolo, additional, Romeo, Irene, additional, Corio, Chiara, additional, Benech, Arnaldo, additional, Ruslin, Muhammad, additional, Forouzanfar, Tymour, additional, Rodríguez-Santamarta, Tanía, additional, Vicente, Juan Carlos de, additional, Tarle, Marko, additional, Dediol, Emil, additional, Pechalova, Petia, additional, Pavlov, Nikolai, additional, Daskalov, Hristo, additional, Doykova, Iva, additional, Kelemith, Kadri, additional, Tamme, Tiia, additional, Kopchak, Andrey, additional, Shumynskyi, Ievgen, additional, Corre, Pierre, additional, Bertin, Helios, additional, Bourry, Maeva, additional, Guyonvarc’h, Pierre, additional, Dovšak, Tadej, additional, Vozlič, David, additional, Birk, Anže, additional, Aničić, Boban, additional, Konstantinovic, Vitomir S., additional, and Starch-Jensen, Thomas, additional

Published
2019
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21. Associations between antimicrobial use and the faecal resistome on broiler farms from nine European countries

Author

Luiken, Roosmarijn, van Gompel, Liese, Munk, Patrick, Sarrazin, Steven, Joosten, Philip, Dorado-García, Alejandro, Borup Hansen, Rasmus, Knudsen, Berith, Bossers, Alex, Wagenaar, Jaap, Aarestrup, Frank, Dewulf, Jeroen, Mevius, Dik, Heederik, Dick, Smit, Lidwien, Schmitt, Heike, Graveland, Haitske, Vanessen, Alieda, Gonzalez-Zorn, Bruno, Moyano, Gabriel, Sanders, Pascal, Chauvin, Claire, David, Julie, Battisti, Antonio, Caprioli, Andrea, Blaha, Thomas, Wadepohl, Katharina, Brandt, Maximiliane, Hald, Tine, Ribeiro Duarte, Ana Sofia, Wasyl, Dariusz, Skarzyńska, Magdalena, Zajac, Magdalena, Daskalov, Hristo, Saatkamp, Helmut, Stärk, Katharina, One Health Microbieel, dI&I I&I-4, Dep IRAS, dIRAS RA-I&I RA, Part of Wageningen UR, Central Veterinary Institute, Obstetrics, Reproduction and Herd Health, Universiteit Gent = Ghent University (UGENT), Environmental and Occupational Health Group, Wageningen University and Research [Wageningen] (WUR), Division of Environmental Epidemiology [Utrecht, The Netherlands], Utrecht University [Utrecht]- Institute of Risk Assessment Sciences [Utrecht, The Netherlands] (IRAS), Institute for Risk Assessment Sciences [Utrecht, The Netherlands] (IRAS), Utrecht University [Utrecht], Facultad de Veterinaria, Dpto. de Sanidad Animal - VISAVET, Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Laboratoire de Fougères - ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Pharmacoepidemiologie et évaluation de l'impact des produits de santé sur les populations, Université Bordeaux Segalen - Bordeaux 2-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Istituto Zooprofilattico Sperimentale del Lazio e della Toscana (IZSLT), Department of Microbiology, National Veterinary Research Institute [Pulawy, Pologne] (NVRI), European Project: 613754,EC:FP7:KBBE,FP7-KBBE-2013-7-single-stage,EFFORT(2013), Universiteit Gent = Ghent University [Belgium] (UGENT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bordeaux Segalen - Bordeaux 2-Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Normandie Université (NU)

Subjects
Veterinary medicine, antibiotic resistance, Epidemiology, animal diseases, Biosecurity, Drug resistance, veterinary drug, antibiotique, Anti-Infective Agents, Risk Factors, Pharmacology (medical), Veterinary drug, bacteria, Original Research, agriculture, 2. Zero hunger, bactérie, 0303 health sciences, Lincosamides, Microbiota, poultry, Bacteriologie, Bacteriology, Host Pathogen Interaction & Diagnostics, Antimicrobial, usage, médicamentvétérinaire, Europe, Infectious Diseases, farm, Microbiology (medical), Farms, medicine.drug_class, Bioinformatica & Diermodellen, Biology, 03 medical and health sciences, Antibiotic resistance, Drug Resistance, Bacterial, Bio-informatics & Animal models, medicine, Animals, Life Science, Epidemiology, Bio-informatics & Animal models, antimicrobial resistance, microbiologie, 030304 developmental biology, Pharmacology, Host Pathogen Interaction & Diagnostics, Epidemiologie, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, 030306 microbiology, microbiology, Computational Biology, Bacteriology, antimicrobial use, Host Pathogen Interactie & Diagnostiek, Resistome, volaille, Cross-Sectional Studies, feces, Epidemiologie, Bioinformatica & Diermodellen, Bacteriologie, Host Pathogen Interactie & Diagnostiek, Flock, Metagenomics, antimicrobien, Chickens
Abstract

Objectives To determine associations between farm- and flock-level antimicrobial usage (AMU), farm biosecurity status and the abundance of faecal antimicrobial resistance genes (ARGs) on broiler farms. Methods In the cross-sectional pan-European EFFORT study, conventional broiler farms were visited and faeces, AMU information and biosecurity records were collected. The resistomes of pooled faecal samples were determined by metagenomic analysis for 176 farms. A meta-analysis approach was used to relate total and class-specific ARGs (expressed as fragments per kb reference per million bacterial fragments, FPKM) to AMU (treatment incidence per DDD, TIDDDvet) per country and subsequently across all countries. In a similar way, the association between biosecurity status (Biocheck.UGent) and the resistome was explored. Results Sixty-six (38%) flocks did not report group treatments but showed a similar resistome composition and roughly similar ARG levels to antimicrobial-treated flocks. Nevertheless, we found significant positive associations between β-lactam, tetracycline, macrolide and lincosamide, trimethoprim and aminoglycoside antimicrobial flock treatments and ARG clusters conferring resistance to the same class. Similar associations were found with purchased products. In gene-level analysis for β-lactams and macrolides, lincosamides and streptogramins, a significant positive association was found with the most abundant gene clusters blaTEM and erm(B). Little evidence was found for associations with biosecurity. Conclusions The faecal microbiome in European broilers contains a high diversity of ARGs, even in the absence of current antimicrobial selection pressure. Despite this, the relative abundance of genes and the composition of the resistome is positively related to AMU in European broiler farms for several antimicrobial classes.

Published
2019

22. Erratum to: Abundance and diversity of the faecal resistome in slaughter pigs and broilers in nine European countries (Nature Microbiology, (2018), 3, 8, (898-908), 10.1038/s41564-018-0192-9)

Author

Munk, Patrick, Knudsen, Berith Elkær, Lukjancenko, Oksana, Duarte, Ana Sofia Ribeiro, Van Gompel, Liese, Luiken, Roosmarijn E.C., Smit, Lidwien A.M., Schmitt, Heike, Garcia, Alejandro Dorado, Hansen, Rasmus Borup, Petersen, Thomas Nordahl, Bossers, Alex, Ruppé, Etienne, Lund, Ole, Hald, Tine, Pamp, Sünje Johanna, Vigre, Håkan, Heederik, Dick, Wagenaar, Jaap A., Mevius, Dik, Aarestrup, Frank M., Graveland, Haitske, van Essen, Alieda, Gonzalez-Zorn, Bruno, Moyano, Gabriel, Sanders, Pascal, Chauvin, Claire, David, Julie, Battisti, Antonio, Caprioli, Andrea, Dewulf, Jeroen, Blaha, Thomas, Wadepohl, Katharina, Brandt, Maximiliane, Wasyl, Dariusz, Skarzyńska, Magdalena, Zajac, Magdalena, Daskalov, Hristo, Saatkamp, Helmut W., Stärk, Katharina D.C., dIRAS RA-I&I I&I, One Health Microbieel, dIRAS RA-I&I RA, Dep IRAS, dI&I I&I-4, and LS Klinisch Onderzoek Wagenaar

Subjects
Coronacrisis-Taverne
Abstract

In the version of this Article originally published, the surname of author Oksana Lukjancenko was spelt incorrectly as ‘Lukjacenko’. This has now been corrected.

Published
2018

23. Abundance and diversity of the fecal resistome in slaughter pigs and broilers in nine European countries

Author

Munk, Patrick, Knudsen, Berith Elkær, Lukjacenko, Oksana, Duarte, Ana Sofia Ribeiro, Van Gompel, Liese, Luiken, Roosmarijn E.C., Smit, Lidwien A.M., Schmitt, Heike, Garcia, Alejandro Dorado, Hansen, Rasmus Borup, Petersen, Thomas Nordahl, Bossers, Alex, Ruppé, Etienne, Graveland, Haitske, van Essen, Alieda, Gonzalez-Zorn, Bruno, Moyano, Gabriel, Sanders, Pascal, Chauvin, Claire, David, Julie, Battisti, Antonio, Caprioli, Andrea, Dewulf, Jeroen, Blaha, Thomas, Wadepohl, Katharina, Brandt, Maximiliane, Wasyl, Dariusz, Skarzyńska, Magdalena, Zajac, Magdalena, Daskalov, Hristo, Saatkamp, Helmut W., Stärk, Katharina D.C., Lund, Ole, Hald, Tine, Pamp, Sünje Johanna, Vigre, Håkan, Heederik, Dick, Wagenaar, Jaap A., Mevius, Dik, Aarestrup, Frank M., One Health Microbieel, LS Klinisch Onderzoek Wagenaar, Dep IRAS, dIRAS RA-I&I RA, dIRAS RA-I&I I&I, dI&I I&I-4, Institute for Risk Assessment Sciences [Utrecht, The Netherlands] (IRAS), Utrecht University [Utrecht], Part of Wageningen UR, Central Veterinary Institute, Genomic Research Laboratory [Geneva, Switzerland], Geneva University Hospital (HUG), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire de Ploufragan - Plouzané, Center for Biological Sequence Analysis, Technical University of Denmark [Lyngby] (DTU), Division of Occupational and Environmental Health, Institute for Risk Assessment (IRAS), EFFORT Group, One Health Microbieel, LS Klinisch Onderzoek Wagenaar, Dep IRAS, dIRAS RA-I&I RA, dIRAS RA-I&I I&I, and dI&I I&I-4

Subjects
0301 basic medicine, pig, Veterinary medicine, antibiotic resistance, Epidemiology, Swine, WASS, Applied Microbiology and Biotechnology, Feces, Environmental DNA, animal, proc, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, bactérie, 0303 health sciences, poultry, Bacteriologie, Bacteriology, Host Pathogen Interaction & Diagnostics, Biodiversity, Poultry farming, Human morbidity, Europe, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Livestock, Gene pool, Microbiology (medical), Bioinformatica & Diermodellen, Immunology, Bedrijfseconomie, Coronacrisis-Taverne, Biology, 03 medical and health sciences, Antibiotic resistance, SDG 3 - Good Health and Well-being, Bacterial Proteins, Species Specificity, Business Economics, Bio-informatics & Animal models, Drug Resistance, Bacterial, Genetics, Life Science, Animals, Epidemiology, Bio-informatics & Animal models, microbiologie, 030304 developmental biology, Host Pathogen Interaction & Diagnostics, Epidemiologie, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Bacteria, 030306 microbiology, business.industry, résistane aux antibiotiques, Gene Expression Profiling, microbiology, Bacteriology, Cell Biology, Sequence Analysis, DNA, Host Pathogen Interactie & Diagnostiek, Biotechnology, Resistome, volaille, 030104 developmental biology, Metagenomics, Epidemiologie, Bioinformatica & Diermodellen, Bacteriologie, Host Pathogen Interactie & Diagnostiek, business, Chickens
Abstract

EFFORT groupHaitske Graveland, Alieda van Essen, Bruno Gonzalez-Zorn, Gabriel Moyano, Pascal Sanders, Claire Chauvin, Julie David, Antonio Battisti, Andrea Caprioli, Jeroen Dewulf, Thomas Blaha, Katharina Wadepohl, Maximiliane Brandt, Dariusz Wasyl, Magdalena Skarzyñska, Magdalena Zajac, Hristo Daskalov, Helmut W Saatkamp, Katharina D.C. Stärk.AbstractAntimicrobial resistance (AMR) in bacteria and associated human morbidity and mortality is increasing. Use of antimicrobials in livestock selects for AMR that can subsequently be transferred to humans. This flow of AMR between reservoirs demands surveillance in livestock as well as in humans. As part of the EFFORT project (www.effort-against-amr.eu), we have quantified and characterized the acquired resistance gene pools (resistomes) of 181 pig and 178 poultry farms from nine European countries, generating more than 5,000 gigabases of DNA sequence, using shotgun metagenomics. We quantified acquired AMR using the ResFinder database and a database constructed for this study, consisting of AMR genes identified through screening environmental DNA. The pig and poultry resistomes were very different in abundance and composition. There was a significant country effect on the resistomes, more so in pigs than poultry. We found higher AMR loads in pigs, while poultry resistomes were more diverse. We detected several recently described, critical AMR genes, includingmcr-1andoptrA, the abundance of which differed both between host species and countries. We found that the total acquired AMR level, was associated with the overall country-specific antimicrobial usage in livestock and that countries with comparable usage patterns had similar resistomes. Novel, functionally-determined AMR genes were, however, not associated with total drug use.

Published
2017
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24. Characteristics of Staphylococcus aureusIsolated from a Case of Foodborne Outbreak in Bulgaria

Author

Ivanova, Teodora, Krumova-Valcheva, Gergana, Mateva, Gergana, and Daskalov, Hristo

Abstract

This paper presents an outbreak provoked by methicillin-resistant strains of coagulase-positive S. aureus(CPS), produced staphylococcal enterotoxins (se) in pig meatballs and potato salad consumed from 70 people in the village Mamarchevo, Bulgaria. Eighteen women aged 50 to 70 years, and two children aged 4 and 5 years have demonstrated a severe malaise with vomiting and indigestion. Two food samples and isolates of CPS were received in the laboratory of Bulgarian Food Safety Agency. Both samples were found to have a high level of CPS. The level of S. aureuscontamination in the potato salad was 8.3 logs CFU/g and 7.7 logs CFU/g in roasted meatballs, which was a significant reason to doubt the production of the toxin. The samples were analyzed according to the European Screening Method v5 using mini VIDAS SET2. The results showed a presence of staphylococcal enterotoxin (TV 2.67 for meatballs and TV 3.27 for potato salad), which was the reason for the ensuing food intoxication. EURL CPS applying quantitative indirect sandwich-type ELISA confirmed the presence of sea, secand sedin the potato salad and sea and sed in the roasted meatballs. Two CPS isolates were confirmed as S. aureusby a species-specific 23S rRNA targeted PCR test. Real-time PCR method detected sea, sed, seg, sei, sej, and sergenes in S. aureusstrains, found in both matrixes. Multiplex PCR method proved the existence of the mecAgene in both S. aureusstrains. Resistance to cefoxitin (>16 mg/L), penicillin (>2 mg/L), kanamycin (64 mg/L) and sulfamethoxazole (>512 mg/L) was found.

Published
2020
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25. Author Correction: Abundance and diversity of the faecal resistome in slaughter pigs and broilers in nine European countries

Author

Munk, Patrick, Knudsen, Berith Elkær, Lukjancenko, Oksana, Duarte, Ana Sofia Ribeiro, Van Gompel, Liese, Luiken, Roosmarijn E.C., Smit, Lidwien A.M., Schmitt, Heike, Garcia, Alejandro Dorado, Hansen, Rasmus Borup, Petersen, Thomas Nordahl, Bossers, Alex, Ruppé, Etienne, Lund, Ole, Hald, Tine, Pamp, Sünje Johanna, Vigre, Håkan, Heederik, Dick, Wagenaar, Jaap A., Mevius, Dik, Aarestrup, Frank M., Graveland, Haitske, van Essen, Alieda, Gonzalez-Zorn, Bruno, Moyano, Gabriel, Sanders, Pascal, Chauvin, Claire, David, Julie, Battisti, Antonio, Caprioli, Andrea, Dewulf, Jeroen, Blaha, Thomas, Wadepohl, Katharina, Brandt, Maximiliane, Wasyl, Dariusz, Skarzyńska, Magdalena, Zajac, Magdalena, Daskalov, Hristo, Saatkamp, Helmut W., Stärk, Katharina D.C., Institute for Risk Assessment Sciences [Utrecht, The Netherlands] (IRAS), Utrecht University [Utrecht], Part of Wageningen UR, Central Veterinary Institute, Genomic Research Laboratory [Geneva, Switzerland], Geneva University Hospital (HUG), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire de Ploufragan - Plouzané, and EFFORT Group

Subjects
pig, 0301 basic medicine, Microbiology (medical), antibiotic resistance, Epidemiology, Bioinformatica & Diermodellen, media_common.quotation_subject, Bedrijfseconomie, Immunology, Zoology, WASS, résistance aux antibiotiques, Biology, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, Antibiotic resistance, Microbial ecology, Business Economics, Abundance (ecology), Bio-informatics & Animal models, Genetics, Life Science, Epidemiology, Bio-informatics & Animal models, animal, microbiologie, bacteria, ComputingMilieux_MISCELLANEOUS, media_common, Host Pathogen Interaction & Diagnostics, Epidemiologie, bactérie, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, poultry, Bacteriologie, Bacteriology, Bacteriology, Host Pathogen Interaction & Diagnostics, Cell Biology, Host Pathogen Interactie & Diagnostiek, Resistome, volaille, Europe, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, 030104 developmental biology, feces, Epidemiologie, Bioinformatica & Diermodellen, Bacteriologie, Host Pathogen Interactie & Diagnostiek, porc, Diversity (politics)
Abstract

In the version of this Article originally published, the surname of author Oksana Lukjancenko was spelt incorrectly as ‘Lukjacenko’. This has now been corrected.

Published
2018

26. Use of multiple-locus variable-number of tandem repeats analysis (MLVA) to investigate genetic diversity ofSalmonella entericasubsp.entericaserovar Typhimurium isolates from human, food, and veterinary sources

Author

Mateva, Gergana, primary, Pedersen, Karl, additional, Sørensen, Gitte, additional, Asseva, Galina, additional, Daskalov, Hristo, additional, Petrov, Petar, additional, Kantardjiev, Todor, additional, Alexandar, Irina, additional, and Löfström, Charlotta, additional

Published
2017
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32. CASE STUDY OF STAPHYLOCOCCAL ENTEROTOXIN POISONING AFTER CONSUMPTION OF READY-TO-EAT ROASTED CHICKEN PRODUCTS.

Author

IVANOVA, TEODORA, GUROVA-MEHMEDOVA, EVA, and DASKALOV, HRISTO

Subjects
STAPHYLOCOCCUS aureus, MEAT microbiology, CHICKENS, FOOD poisoning, FOOD contamination
Abstract

Staphylococcus aureus is a food-borne pathogen, capable to produce several enterotoxins (SEs) that cause intoxication with varying intensity in humans after ingestion of contaminated food. The present paper reports the results of an outbreak, provoked by Coagulase Positive Staphylococci (CPS) and enterotoxins in ready-to-eat chicken products, occurred in city of Yambol (Bulgaria) in January 2015. An old woman and a child were hospitalized after consumption of ready-to-eat roasted chicken legs. A total of 5 samples were examined and one of them was found to contain high level of CPS. The level of Staphylococcus aureus contamination in the positive sample was 6.92 log CFU/g, which was a significant reason to suspect the production of toxin. The sample with high level of contamination was analyzed according to the European Screening Method v5 using mini VIDAS SET2. The results showed presence of staphylococcal enterotoxin (TV 1.16), which was the cause for the ensuing food intoxication. EURL for coagulase positive staphylococci confirmed presence of SEA at a level of 0.033 ng/g applying quantitative indirect sandwich-type ELISA. Two CPS isolates were confirmed as S. aureus by a species-specific 23S rRNA targeted PCR test. Both isolates carried the sea gene only. [ABSTRACT FROM AUTHOR]

Published
2017

43. Microbiological status of fish products on retail markets in the Republic of Bulgaria.

Author

Stratev, Deyan, Vashin, Ivan, and Daskalov, Hristo

Subjects
FISH as food microbiology, RETAIL industry, FOOD storage, ROACH (Fish)
Abstract

The purpose of the study was to determine the microbiological status of 161 samples cooled and frozen fish products collected from retail markets in the Republic of Bulgaria. The highest total viable counts (TVCs) in cooled fish products were established for silver carp (7.30 log cfu/g), Black sea roach (7.28 log cfu/g) and trout (7.11 log cfu/g), followed by common carp (6.81 log cfu/g), vacuum packed trout fillets (6.39 log cfu/g) and horse mackerel (5.90 log cfu/g). The highest Aeromonas spp. load was established in cooled trout (5.89 log cfu/g) and vacuum packed trout fillets (5.63 log cfu/g), followed by cooled silver carp (4.95 log cfu/g), common carp (4.09 log cfu/g) and horse mackerel (2.68 log cfu/g). Among frozen fish products, the highest microbial contamination was detected in frozen sprats (4.43 log cfu/g), followed by trout (4.27 log cfu/g) and mackerel (4.10 log cfu/g). Aeromonas spp. were not found out in Black sea roach and frozen fish samples. The relative proportion of Aeromonas spp. out of total microbial counts was the highest for vacuum-packed trout fillets (88.1%), followed by trout (82.8%), silver carp (67.8%), common carp (60.1%) and horse mackerel (45.4%). Cooled silver carp, Black sea roach and trout were highly contaminated with microorganisms. The significant number of contaminated cooled fish products and the high load of Aeromonas spp. pose a risk for the health of consumers. [ABSTRACT FROM AUTHOR]

Published
2015

47. NEAR INFRARED SPECTROSCOPY (NIR) AS A POTENTIAL TOOL FOR EFFECTIVE FAST DETECTION OF FOODBORNE PATHOGEN LISTERIA MONOCYTOGENES IN FRANKFURTERS.

Author

Daskalov, Hristo, Atanasova, Stefka, and Stoyanchev, Todor

Subjects
*NEAR infrared spectroscopy, *FOOD microbiology, *FOOD pathogens, *SURFACE contamination, *MICROBIOLOGY, *SALMONELLA, *LISTERIA
Abstract

A current project connected with application of near infrared (NIR) spectroscopy in microbiology and food controlls briefly introduced. Fast and cheap determination of small number of cells on the surface of foods has a big influence on effectiveness of veterinary control in food chain. The activities of the project include investigation of possibilities of NIR spectroscopy for detection of surface contamination of foodstuffs with some important for human health foodborne pathogens such as Salmonella spp., Listeria spp. and others. As an example of the project activities some recent results are presented IVIR spectroscopy in combination with multivariate chemometrics techniques could be used for development of a new method for detection of contaminated with Listeria monocytogenes frankfurters. [ABSTRACT FROM AUTHOR]

Published
2008

48. Synthesis and Properties of O6-Substituted Guanosine Derivatives

Author

Daskalov, Hristo Petrov, Sekine, Mitsuo, and Hata, Tsujiaki

Abstract

Reactions of 2′,3′,5′-tri-O-acetyl-N2-protected or unprotected guanosine derivatives with phosphoryl, phosphinothioyl, arylsulfonyl, and silyl halides in dichloromethane gave the corresponding O6-substituted guanosine derivatives in good yields. In these reactions, 4-(dimethylamino)pyridine (DMAP) was found to be very effective catalyst. The physical data of these products and their stabilities under acidic and basic conditions were described in detail. Selective detritylation of O6-arylsulfonyl-N2-tritylguanosine derivatives was accomplished by treatment with 80% acetic acid. It was found that O6-dibutylphosphinothioyl derivatives were relatively stable under conditions where acetyl group was predominantly removed.

Published
1981
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