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Background: Abelmoschus ficulneus(L.) Wight & Arn. (Malvaceae) commonly known as herb in the Ayurveda, Indian traditional medicine system. Herb is reported as a Rasayana that has the potential to produce immunomodulatory effects. In the present study, the immunomodulatory effect of aqueous (AEAF) and ethanolic (EEAF) leaves extract of A. ficulneus(L.) Wight & Arn. in different experimental animal models were evaluated. Results: Acute oral toxicity shows that both extracts have wide range of safety as per OECD 420 guidelines. Quantitative and qualitative analysis was evaluated by performing preliminary phytochemical screening, thin layer chromatography and high-performance thin layer chromatography developed for simultaneous quantification of flavonoids (quercetin) in both the extracts. Oral administration of A. ficulneusL. extracts showed a significant increase in the primary and secondary humoral antibody responses against cyclophosamide (100 mg/kg) induced immunosuppression, by significantly increasing (p< 0.05) the hemagglutinating antibody titre at dose of 200 mg/kg/p.o. and also significantly (p< 0.05) potentiated delayed type hypersensitivity reaction induced by sheep red blood cells. There was a significant (p< 0.05) increase in the phagocytic index, percentage neutrophil adhesion and significantly prevented the mortality induced by bovine Pasteurella multocidain mice when compared with control group. Result findings shows that both the extracts have ability to inhibit immunosuppression induced by cyclophosamide. Conclusion: Study finding shows that AEAF and EEAF (at dose 200 mg/kg) extracts exerts immunostimulatory effects on specific and nonspecific immune system thus chemical constituents of herbal extracts may have applications as an effective immunotherapeutic agent and its potential application in the treatment of immune-related diseases.