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Spermatogenesis is a complex process. It is the modification of progenitor spermatogonia into mature spermatozoa. The stages are similar in all-male vertebrates, as well as avian species. However, studies on spermatogenesis in birds are fewer compared to mammals. The current study investigated the ultrastructural changes in the spermatogenic cells of domestic chickens in different reproductive stages. Thirty (30) male birds, ten (10) in each of the three reproductive stages: pre-pubertal, pubertal, and adult were used in the study. Testicular tissues from all age groups were processed for transmission electron microscopy (TEM). TEM results showed spermatogonia and primary spermatocytes in the pre-pubertal testis, and the seminiferous tubule lumen was wide and empty. Also, the nuclei of spermatogonia at this stage did not contain condensed chromatin material at the center nor scattered at the periphery of the nuclear membrane. There were slight differences between the spermatogenic cells in the pubertal and adult age groups. The spermatogonia, primary and secondary spermatocytes, and round spermatids with scanty chromatin material were observed in both age groups. In the adult age group, round and elongated spermatids with condensed chromatin materials were observed besides the other spermatogenic cells. Also, the seminiferous tubule lumen was filled with sperm cells and cellular debris, unlike in the pre-pubertal and pubertal age groups where they were wide and empty. The presence of numerous oval mitochondria were observed in all age groups. This signifies the active process of spermatogenesis in pre-pubertal, pubertal, and adult male domestic chickens., Competing Interests: Declaration of Competing Interest The authors have no competing interests to declare., (Copyright © 2024 Elsevier Ltd. All rights reserved.)