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Duggleby (2018) has made a numerical analysis of some aspects of the wide range of phenomena we reviewed in Steele et al. (2018) and asserted " .that panspermia as proposed by Steele et al. (2018) is extremely implausible.” It seems to us that Duggleby has based his viewpoint on a quite narrow and specific model of Panspermia which he supposes to be active in the cosmos. Here we address both his conclusions and his numerical analysis. Our response therefore will be at two levels, his specific analysis and his general conclusions. In the specific section below we show that while Duggleby's numerical analysis appears in part correct it is, in the final analysis, quite irrelevant to Cosmic Panspermia. In the general response which follows we address his unsupported conclusion throughout his critique, namely that … " none of the examples mentioned by Steele et al. (2018) is decisive enough to allow no other explanation."

We review the salient evidence consistent with or predicted by the Hoyle-Wickramasinghe (H-W) thesis of Cometary (Cosmic) Biology. Much of this physical and biological evidence is multifactorial. One particular focus are the recent studies which date the emergence of the complex retroviruses of vertebrate lines at or just before the Cambrian Explosion of ∼500 Ma. Such viruses are known to be plausibly associated with major evolutionary genomic processes. We believe this coincidence is not fortuitous but is consistent with a key prediction of H-W theory whereby major extinction-diversification evolutionary boundaries coincide with virus-bearing cometary-bolide bombardment events. A second focus is the remarkable evolution of intelligent complexity (Cephalopods) culminating in the emergence of the Octopus. A third focus concerns the micro-organism fossil evidence contained within meteorites as well as the detection in the upper atmosphere of apparent incoming life-bearing particles from space. In our view the totality of the multifactorial data and critical analyses assembled by Fred Hoyle, Chandra Wickramasinghe and their many colleagues since the 1960s leads to a very plausible conclusion - life may have been seeded here on Earth by life-bearing comets as soon as conditions on Earth allowed it to flourish (about or just before 4.1 Billion years ago); and living organisms such as space-resistant and space-hardy bacteria, viruses, more complex eukaryotic cells, fertilised ova and seeds have been continuously delivered ever since to Earth so being one important driver of further terrestrial evolution which has resulted in considerable genetic diversity and which has led to the emergence of mankind. ispartof: PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY vol:136 pages:3-23 ispartof: location:England status: published

The space density of life-bearing primordial planets in the solar vicinity may amount to ∼8.1×104 pc−3 giving total of ∼1014 throughout the entire galactic disk. Initially dominated by H2 these planets are stripped of their hydrogen mantles when the ambient radiation temperature exceeds 3 K as they fall from the galactic halo to the mid-plane of the galaxy. The zodiacal cloud in our solar system encounters a primordial planet once every 26 My (on our estimate) thus intercepting an average mass of 103 tonnes of interplanetary dust on each occasion. If the dust included microbial material that originated on Earth and was scattered via impacts or cometary sublimation into the zodiacal cloud, this process offers a way by which evolved genes from Earth life could become dispersed through the galaxy.

We report for the first time in situ observations of 5-50μm spherical carbonaceous structures in the Tissint Martian meteorite comprising of pyrite (FeS 2 ) cores and carbonaceous outer coatings. The structures are characterized as smooth immiscible spheres with curved boundaries occasionally following the contours of the pyrite inclusion. The structures bear striking resemblance to similar-sized immiscible carbonaceous spheres found in hydrothermal calcite vein deposits in the Mullaghwornia Quarry in central Ireland. Similar structures have been reported in Proterozoic and Ordovician sandstones from Canada as well as in a variety of astronomical sources including carbonaceous chondrites, chondritic IDPs and primitive chondritic meteorites. SEM and X-Ray elemental mapping confirmed the presence of organic carbon filling the crack and cleavage space in the pyroxene substrate, with further evidence of pyrite acting as an attractive substrate for the collection of organic matter. The detection of precipitated carbon collecting around pyrite grains is at variance with an igneous origin as proposed for the reduced organic component in Tissint, and is more consistent with a biogenetic origin.

Earlier studies of the Tissint Martian meteorite identified the presence of a number of 5-50μm carbonaceous spherical structures. SEM and EDS elemental spectra for 11 selected structures confirmed that they comprise of a carbonaceous outer coating with a inner core of FeS 2 (pyrite) and are characterised as immiscible globules with curved boundaries. Here we report on the results of Raman spectroscopic studies that unambiguously confirm the mantle as comprising of ‘disordered carbonaceous material’. R1 = I D /I G against Γ D (cm -1 ) band parameter plots of the carbonaceous coatings imply a complex precursor carbon inventory comparable to the precursor carbon component of materials of known biotic source (plants, algae, fungi, crustaceans, prokaryotes). Correlation between peak metamorphic temperatures and Raman D-band (Γ D ) parameters further indicate the carbonaceous component was subjected to a peak temperature of ~250 O C suggesting a possible link with the hydrothermal precipitation processes responsible for the formation of similar globules observed in hydrothermal calcite veins in central Ireland. Ω G (cm -1 ), Γ G (cm -1 ), Ω D (cm -1 ) and Γ D (cm -1 ) parameters further imply a level of crystallinity and disorder of the carbon component consistent with carbonaceous material recovered from a variety of non-terrestrial sources. Cl, N, O and S to C elemental ratios are typical of high volatility bituminous coals and distinctly higher than equivalent graphite standards.

The theory of cometary panspermia is reviewed in relation to evidence from astronomy, biology and recent studies of meteorites. The spectroscopic signatures in interstellar material within our galaxy and in external galaxies that have been known for many years most plausibly represent evidence for the detritus of life existing on a cosmic scale. Such spectral features discovered in galaxies of high redshift points to life arising at a very early stage in the history of the Universe. Evidence of fossils of microscopic life forms in meteorites that have been discussed over several decades, and augmented recently with new data, reaffirms the case for cometary panspermia.

A new modified Kramers Kronig Integral is derived and shown to produce excellent results when k data is only known over a limited range. By considering the effect of resonance features simulated using the Dirac-Delta function, the new integral is shown to be more rapidly converging than both the conventional Kramers Kronig integral and a modified (Subtractive Kramers Kronig – SKK) integral introduced by Ahrenkiel (1971). The new integral does not require extensive extrapolation of reflectance data outside the measured region in order to produce reliable results. By extending the above procedure to include n data points, it is shown that at wavelength λ0, \[ n(λ_0)=\sum_{i=1}^{\rm n}(-1)^{\rm n+1}\prod_{\stackrel{j=1}{j \not=i}}^{\rm n} \frac{(λ_j^2-λ_0^2)}{(λ_i^2- λ_j^2)}n(λ_i)+\frac{2}{\pi}P\int_{0}^{\infty}(-1)^{\rm n+1} \frac{\prod_{i=1}^{\rm n}(λ_i^2-λ_0^2)}{\prod_{i=0}^{\rm n}(λ^2-λ_i^2)}λ k(λ)dλ \] with relative error given by, \[ R_n(λ_0)=\prod_{i=1}^{\rm n}\frac{λ_i^2- λ_0^2}{λ_Σ^2-λ_i^2} . \] This nth order expression should prove useful in establishing the internal self-consistency of data sets for which both optical coefficients have been theoretically derived.

Data on infrared spectra of astronomical sources and on the unidentified infrared bands is reviewed and compared with a range of predictions from organic models of interstellar grains. The best fits to a wide range of data appear to require the widespread occurrence of functional groups distributed in exactly the proportions that characterise biosystems. The question of whether these agreements are consistent with extraterrestrial prebiology, or even cosmic biology is discussed.

Scattering models of aerosol particles at the G-impact site (18 July1994) are presented for a number of likely compositional candidates. Two differing dust particle population distribution functions are taken, along with varying aerosol cloud densities, leading to differing optical depths. A number of models including graphite, amorphous carbon, astrophysical silicate, water ice and a number of organic compounds are discussed, but no single material provides a fully satisfactory fit to the published observations. A porous silicate/graphite composite is found to provide a good fit to the spectral data.

On December 29, 2012, a bright yellow and green fireball was observed to disintegrate over the Polonnaruwa District of North Central, Sri Lanka. Many low density, black stones were recovered soon after the observed fall from rice paddy fields near the villages of Aralaganwila and Dimbulagala. These stones were initially studied by optical microscopy methods at the Medical Research Institute in Colombo, Sri Lanka. Soon thereafter, samples were sent to the UK and to the United States. More extensive Field Emission Scanning Electron Microscopy studies were then carried out at Cardiff University and the NASA/Marshall Space Flight Center. The physico-chemical properties, elemental abundances, mineralogy and stable isotope data clearly indicate that these stones are non-terrestrial. Freshly fractured interior surfaces of the black stones have also been observed to contain the remains of fossilized diatom. Many of the diatom frustules are clearly embedded in the meteorite rock matrix and exhibit nitrogen levels below the EDX detection limits. Some of the fossil diatoms are araphid marine pennates and planktonic forms that are inconsistent with conditions associated with rice paddy fields. These observations indicate the fossilized diatoms are indigenous to the meteorites rather than post-arrival biological contaminants. The carbon content and mineralogy suggests that these stones may represent a previously ungrouped clan of carbonaceous meteorites. The extremely low density (~0.6) of the stones and their observed mineralogy was inconsistent with known terrestrial rocks (e.g., pumice, diatomite and fulgurites). The minerals detected suggest that the parent body of the Polonnaruwa stones may have been the nucleus of a comet. These observations are interpreted as supporting the Hoyle-Wickramasinghe Panspermia hypothesis and the hypothesis that diatoms and other microorganisms might be capable of living and growing in water ice and brines in comets.

We report on the physical, chemical and mineral properties of a series of stone fragments recovered from the North Central Province of Sri Lanka following a witnessed fireball event on 29 December 2012. The stones exhibit highly porous poikilitic textures comprising of isotropic silica-rich/plagioclase-like hosts. Inclusions range in size and shape from mm-sized to smaller subangular grains frequently more fractured than the surrounding host and include ilmenite, olivine (fayalitic), quartz and accessory zircon. Bulk mineral compositions include accessory cristobalite, hercynite, anorthite, wuestite, albite, anorthoclase and the high pressure olivine polymorph wadsleyite, suggesting previous endurance of a shock pressure of ~20 GPa. Further evidence of shock is confirmed by the conversion of all plagioclase to maskelynite. Here the infrared absorption spectra in the region 580 cm-1 to 380 cm-1 due to the Si-O-Si or Si-O-Al absorption band shows a partial shift in the peak at 380 cm-1 towards 480 cm-1 indicating an intermediate position between crystalline and amorphous phase. Host matrix chemical compositions vary between samples, but all are rich in SiO2. Silica-rich melts display a heterogeneous K-enrichment comparable to that reported in a range of non-terrestrial material from rare iron meteorites to LL chondritic breccias and Lunar granites. Bulk chemical compositions of plagioclase-like samples are comparable to reported data e.g. Miller Ranger 05035 (Lunar), while Si-rich samples accord well with mafic and felsic glasses reported in NWA 1664 (Howardite) as well as data for fusion crust present in a variety of meteoritic samples. Triple oxygen isotope results show Δ17O = -0.335 with δ18O (‰ rel. SMOW) values of 17.816 ± 0.100 and compare well with those of known CI chondrites and are within the range of CI-like (Meta-C) chondrites. Rare earth elemental abundances show a profound Europium anomaly of between 0.7 and 0.9 ppm while CI normalized REE patterns accord well with those of high potassium and high aluminium glasses found in lunar and 4 Vesta samples. Twoelement discrimination maps of FeO vs SiO2, FeO vs TiO2, FeO vs Al2O3 and FeO vs Na2O similarly match those of impact glasses present in lunar samples and remain within relatively close proximity of the KREEP component. Iridium levels of between 1-7ppm, approximately 104 times that of terrestrial crustal rocks, were detected in all samples.

The optical constants of a carbon polymorph, calculated using the Drude dispersion theory, are shown to lead to close agreements with the mean interstellar extinction curve over the waveband 0.3 ≤ λ-1 ≤ 5.5 μm-1 for particles of Rayleigh scattering sizes. Astrophysical microsoot grains of radii ≤50A may be related to microdiamond grains of similar sizes that have recently been discovered in carbonaceous chondrites. It is postulated that such microsoot particles could contribute to the nearly invariable interstellar extinction law observed over the near IR, visible and near to mid UV spectral regions. Admixtures of microsoot with micron-sized microsoot clumps, microdiamond and hollow organic / biologic grains provide an explanation for the full range of extinction and polarization data.

A mass contribution of graphite whiskers as small as 0.1% to the population of interstellar grains could dramatically change their far-infrared extinction properties. With varying mass fractions of graphite whiskers, and for different size parameters, the infrared extinction could vary from a λ-2 dependence to λ0, consistent with the requirements of some astronomical observations.

Preliminary SEM/EDAX studies of the Tissint meteorite shows projections of interior spherical globules rich in C and O. Such concentrations of carbonaceous material in a matrix of mineral grains pose a mystery. These structures are consistent with remnants of biological structures.

Air samples are to be collected at various altitude sin the stratosphere using balloons flown form Hyderabad, India. The samples will be passed through sterile micropore filters, after which the filters will be analyzed using voltage sensitive lipophilic dyes to detect the presence of either active or non-active cells. Organisms detected in this manner will be studied using static mass spectroscopy to establish isotropic ratios 13C/12C and D/H, which would distinguish between terrestrial and extraterrestrial cells.

Astronomical spectra over a wide range of wavelengths is reviewed and compared with predictions from organic modelsof interstellar and cometary grains. The data requires the widespread occurrence of functional groups involvingH,O,C,N in the form of complex structures in the proportions that characterise biomaterial. We argue for the widespreadoccurrence of a microbiological system on a galaxy-wide scale. 1. EARLY IDEAS For many years two of us (FH and NCW) have developed arguments to support the hypothesis that both interstellar andcometary dust is predominantly organic in character . Our arguments were initially driven by inadequacies of mineralgrains for explaining broad 1Oim and 2Oim features that were first discovered in the spectrum ofthe Trapeziumnebulaand later in other astronomical JR sources including comets . In particular the observed 8-1 3 im emission band ofthe Trapezium nebula stubbornly failed to match spectra of laboratory silicates, whereas admixtures of minerals withorganic polymers, particularly with a C-O-C

The theory of cometary panspermia is reviewed in relation to evidence from astronomy, biology and recent studies of meteorites. The spectroscopic signatures in interstellar material within our galaxy and in external galaxies that have been known for many years most plausibly represent evidence for the detritus of life existing on a cosmic scale. Such spectral features discovered in galaxies of high redshift points to life arising at a very early stage in the history of the Universe. Evidence of fossils of microscopic life forms in meteorites that have been discussed over several decades, and augmented recently with new data, reaffirms the case for cometary panspermia.