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PHYTOTOXIC effects of propamocarb-HCl pesticide and its Nano form on cucumber plant and their adverse impact on tissue culture of HepG2 line were studied. Nano form was loaded on chitosan nanoparticles (NPs). Significant increase in plant morphological parameters was noticed after the last periods of transplanting. Also, it exhibited significant increases in fruits yield greater than the traditional form. The quality recognized the same pattern. All treatments: Doses, ½ doses, and double doses showed an increase in leave pigments in the case of nano-form treatments greater than the traditional form. Biosafety profile on HepG2 cell lines showed a slight difference with 1.22 folds between the traditional and nano-form. While, lactate dehydrogenase (LDH) and malondialdehyde (MDA) showed a significant increase, compared to their control. Such a finding provides the advantage of nano-formation on vegetable crops, but it must be coupled with further toxicological studies. [ABSTRACT FROM AUTHOR]