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14 results on '"Dargis, Michèle"'

5. Safety and immunogenicity of a plant-derived rotavirus-like particle vaccine in adults, toddlers and infants

Author

Kurokawa, Natsuki, primary, Robinson, Michelle K., additional, Bernard, Catherine, additional, Kawaguchi, Yutaka, additional, Koujin, Yoshito, additional, Koen, Anthonet, additional, Madhi, Shabir, additional, Polasek, Thomas M., additional, McNeal, Monica, additional, Dargis, Michèle, additional, Couture, Manon M.-J., additional, Trépanier, Sonia, additional, Forrest, Bruce D., additional, and Tsutsui, Naohisa, additional

Published
2021
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8. Influenza virus-like particles produced by transient expression inNicotiana benthamianainduce a protective immune response against a lethal viral challenge in mice

Author

D’Aoust, Marc-André, primary, Lavoie, Pierre-Olivier, additional, Couture, Manon M.-J., additional, Trépanier, Sonia, additional, Guay, Jean-Martin, additional, Dargis, Michèle, additional, Mongrand, Sébastien, additional, Landry, Nathalie, additional, Ward, Brian J., additional, and Vézina, Louis-P., additional

Published
2008
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9. Monoclonal C5-1 antibody produced in transgenic alfalfa plants exhibits a N-glycosylation that is homogenous and suitable for glyco-engineering into human-compatible structures

Author

Bardor, Muriel, primary, Loutelier-Bourhis, Corinne, additional, Paccalet, Thomas, additional, Cosette, Pascal, additional, Fitchette, Anne-Catherine, additional, Vézina, Louis-P., additional, Trépanier, Sonia, additional, Dargis, Michèle, additional, Lemieux, Réal, additional, Lange, Catherine, additional, Faye, Loïc, additional, and Lerouge, Patrice, additional

Published
2003
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11. Influenza virus-like particles produced by transient expression in Nicotiana benthamiana induce a protective immune response against a lethal viral challenge in mice.

Author

D'Aoust, Marc‐André, Lavoie, Pierre‐Olivier, Couture, Manon M.‐J., Trépanier, Sonia, Guay, Jean‐Martin, Dargis, Michèle, Mongrand, Sébastien, Landry, Nathalie, Ward, Brian J., and Vézina, Louis‐P.

Subjects
VACCINATION, INFLUENZA viruses, NICOTIANA, IMMUNITY, PANDEMICS, CELL membranes, PARTICLES (Nuclear physics)
Abstract

A strain-specific vaccine represents the best possible response to the threat of an influenza pandemic. Rapid delivery of such a vaccine to the world's population before the peak of the first infection wave seems to be an unattainable goal with the current influenza vaccine manufacturing capacity. Plant-based transient expression is one of the few production systems that can meet the anticipated surge requirement. To assess the capability of plant agroinfiltration to produce an influenza vaccine, we expressed haemagglutinin (HA) from strains A/Indonesia/5/05 (H5N1) and A/New Caledonia/20/99 (H1N1) by agroinfiltration of Nicotiana benthamiana plants. Size distribution analysis of protein content in infiltrated leaves revealed that HA was predominantly assembled into high-molecular-weight structures. H5-containing structures were purified and examination by transmission electron microscopy confirmed virus-like particle (VLP) assembly. High-performance thin layer chromatography analysis of VLP lipid composition highlighted polar and neutral lipid contents comparable with those of purified plasma membranes from tobacco plants. Electron microscopy of VLP-producing cells in N. benthamiana leaves confirmed that VLPs accumulated in apoplastic indentations of the plasma membrane. Finally, immunization of mice with two doses of as little as 0.1 µg of purified influenza H5-VLPs triggered a strong immune response against the homologous virus, whereas two doses of 0.5 µg of H5-VLPs conferred complete protection against a lethal challenge with the heterologous A/Vietnam/1194/04 (H5N1) strain. These results show, for the first time, that plants are capable of producing enveloped influenza VLPs budding from the plasma membrane; such VLPs represent very promising candidates for vaccination against influenza pandemic strains. [ABSTRACT FROM AUTHOR]

Published
2008
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12. An ampDGene in Pseudomonas aeruginosaEncodes a Negative Regulator of AmpC β-Lactamase Expression

Author

Langaee, Taimour Yousef, Dargis, Michèle, and Huletsky, Ann

Abstract

ABSTRACTThe ampDand ampEgenes ofPseudomonas aeruginosaPAO1 were cloned and characterized. These genes are transcribed in the same orientation and form an operon. The deduced polypeptide of P. aeruginosa ampDexhibited more than 60% similarity to the AmpD proteins of enterobacteria andHaemophilus influenzae. The ampDproduct transcomplemented Escherichia coli ampDmutants to wild-type β-lactamase expression.

Published
1998
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13. Alterations in penicillin-binding proteins in strains of Streptococcus suis possessing moderate and high levels of resistance to penicillin

Author

Cain, Dean, Malouin, François, Dargis, Michèle, Harel, Josée, and Gottschalk, Marcelo

Abstract

We examined the penicillin-binding proteins (PBPs) of certain field strains of Streptococcus suis, as well as those from laboratory variants having different degrees of resistance to penicillin. Results indicated that (i) S. suis possesses three distinct groups of PBPs, arbitrarily named here PBP 1, PBP 2, and PBP 3, with approximate molecular weights of 97, 82, and 45 kDa respectively; (ii) PBP profiles of field strains of S. suis having different MICs (≤ 0.03 to 16.0 μg/ml) were not uniform (PBP 2 being difficult to detect in strains whose MICs exceeded 0.10 μg/ml, and PBP 3 which exhibited shifts in molecular weight of approximately 5 kDa); (iii) laboratory variant PBPs 1 and 2 showed decreased affinity for penicillin as compared to the parent strain in antibiotic competition experiments, even though the PBP profiles of both were similar. We suggest that PBP modifications (altered molecular weight and/or decreased affinity for penicillin) are involved in the mechanism of resistance to penicillin by S. suis.

Published
1995
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