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Wood and basketry artefacts rarely survive in the prehistoric record since they require exceptional conditions for preservation; as a result, the current knowledge about when and how prehistoric societies used these basic organic raw materials is limited. Focusing on the southern Levant, we discuss for the first time a collection of 16 late prehistoric organic artefacts found in underwater research conducted in the last forty years off the coast of the Carmel Ridge (Israel). The waterlogged finds, including bowls, shafts, a wedge, a trough, a pitchfork, logs, a mat, and a basket, were found at sites spanning from the Pre-Pottery Neolithic to Middle Chalcolithic periods (ninth–seventh millennia cal. BP), constituting an unprecedented record of prehistoric wood and other perishable materials, providing us with new information about raw material preferences and manufacturing technologies.

Fiber technology (cordage and textile) has played a central role in all human societies for thousands of years, and its production, application and exchange have deep roots in prehistory. However, fiber remains have only rarely been observed in prehistoric sites because they tend to decay quickly in normal environmental conditions. To overcome preservation problems of macroscopic remains, we employed microbotanical analysis on soils from anthropogenic sediments in activity areas at Tel Tsaf in the Jordan Valley, Israel (ca. 5,200–4,700 cal BC), and recovered fiber microremains. This includes at least two types of bast fibers and the earliest evidence of cotton in the Near East, some of which were dyed in various colors. Some of these fibers likely represent the remnants of ancient clothing, fabric containers, cordage, or other belongings. The cotton remains, probably derived from wild species originating in South Asia, predate the oldest known cotton domestication in the Indus Valley by about two millennia. Tel Tsaf played a pivotal role in trans-regional trade and exchange networks in the southern Levant, and the presence of cotton at the site points to possible connections with the Indus Valley as early as 7,200 years ago.

Abstract We present the results of a detailed geochemical provenance study of 54 Natufian (ca. 15,000–11,700 cal. BP) basalt pestles from the site of el-Wad Terrace (EWT), Israel. It is the first time precise locations from where basalt raw materials were derived are provided. The results indicate that the Natufian hunter-gatherers used multiple sources of basaltic rocks, distributed over a large area surrounding the Sea of Galilee. This area is located at a considerable distance from EWT, ca. 60–120 km away, in a region where contemporaneous Natufian basecamps are few. We consider two possible models that suggest vehicles for the transportation of these artifacts to EWT, namely the exchange obtaining model (EOM) and the direct procurement model (DPM). We argue that these mechanisms are not mutually exclusive and may have operated together. We also suggest that at a time of increasing Natufian territoriality, a large area around the Sea of Galilee remained unclaimed. The paper concludes with a brief discussion of the implications for the two models. In particular, we note that the DPM implies that technological know-how for pestle production was maintained within the EWT community.

Eight olivine beads found at the Middle Chalcolithic site of Tel Tsaf (ca. 5,200-4,700 cal. BC), Jordan Valley, Israel, underscore a new facet of interregional exchange for this period. The current paper presents the olivine beads assemblage, its morphometric and technological characteristics, and chemical composition. The results of the chemical analysis suggest that all eight beads derive from the same source. By means of comparison with the chemical characteristics of known olivine sources, we argue for a northeastern African-western Arabian provenience and cautiously suggest Ethiopia as a probable origin. Finally, we discuss the significance of the assemblage, its possible origin, and the mechanisms that may have brought the beads to the site.

Beehive products have a rich global history. In the wider Levantine region, bees had a significant role in Egypt and Mesopotamia, and intensive beekeeping was noted in Israel during the Biblical period when apiaries were first identified. This study investigates the origins of this extensive beekeeping through organic residue analysis of pottery from prehistoric sites in the southern Levant. The results suggest that beehive products from likely wild bees were used during the Chalcolithic period as a vessel surface treatment and/or as part of the diet. These functions are reinforced by comparison to the wider archaeological record. While the true frequency of beeswax use may be debated, alternatives to beehive products were seemingly preferred as wild resources contrasted with the socio-economic system centred on domesticated resources, controlled production and standardization. Bee products only became an important part of the economic canon in the southern Levant several millennia later.

One of the most characteristic aspects of the Late Chalcolithic and Early Bronze Age periods in the southern Levant is the appearance of large assemblages of basalt vessels. These vessels, frequently meticulously made, appear sometimes a considerable distance from the raw material sources and are found mainly at habitation sites. While these and their prestigious value have been widely discussed in the past, their function is still obscure. In the current paper, we address their functionality through microscopic use-wear analysis. Emphasis was placed on basalt vessels with a distinct wear pattern-circumferential depressions, which appear along the perimeter of their interior bases. The documented traces were compared to results of an experimental study we conducted to characterize the effects of abrasion, grinding, and lubrication on basalt surfaces. The results of the comparative experimental study suggest that the circumferential depression was formed from a repetitive rotational activity using a narrow-ended tool. Further, it seems that two material types acted in combination as the circling device and processed material. One was hard and abrasive, such as stone, and the other was semi-resilient, such as wood or mineral powder. Water was likely used as a lubricant in the rotational process. While the actual function of the bowls bearing the circumferential depressions is not entirely clear, the use-wear analyses suggest that they may have been devices involved in craft industries, used for processing materials unrelated to food (minerals in particular). Whatever the exact function was, it clear that this use continued from the Chalcolithic through the Early Bronze Age, providing evidence for functional continuity between these two periods.

Late Chalcolithic metallurgy developed in the southern Levant simultaneously with other crafts and new social institutions, reflecting advances in social organization, cults and technology. Until recently, copper items were mostly found in the Negev and Judean Desert, while other areas, specifically the Jordan Valley, were considered poor, with limited copper finds. Recent excavations at Late Chalcolithic Fazael in the Jordan Valley yielded dozens of copper items that allow for the first time a comprehensive study of copper items from this area. The assemblage is one of the largest of any site in the Late Chalcolithic period and includes most of the known components of the Late Chalcolithic copper industry. The current paper presents the new metallurgical discoveries from the Fazael Basin and discusses their significance to our understanding of the Late Chalcolithic copper industry.

The history of mudbrick production and construction in the southern Levant may be dated as far back to the Pre-Pottery Neolithic A. However, at many of the sites where mudbrick remains were noted, their preservation was poor, so investigation of their production and the related construction techniques in antiquity was precluded. The 7,200 year old (cal BP) site of Tel Tsaf, located in the Jordan Valley, is distinguished by outstanding preservation of mudbrick architecture, which enables us to delve into various issues related to mudbrick technology, construction and preservation. The present paper discusses some of the mudbrick features at Tel Tsaf and their characteristics and offers a comprehensive analytical study of the mudbricks from multiple contexts and phases. These demonstrate consistency in three of the four measured variables: magnetic susceptibility, organic content and calcium carbonate equivalent. The results of our study suggest that while we can identify morphometric variability between bricks and walls, by and large, a uniform composition characterized the tested assemblages without any temporal or spatial variability. This indicates that a single locally-sourced raw material was used and that recycling of old decayed mudbricks was likely practiced. The consistency of mudbrick-production during all phases of the occupation at Tel Tsaf and the absence of multiple recipes implies that a shared production and technological know-how was maintained for at least 500 years at the site.

SUMO proteases of the SENP/Ulp family are master regulators of both sumoylation and desumoylation and regulate SUMO homeostasis in eukaryotic cells. SUMO conjugates rapidly increase in response to cellular stress, including nutrient starvation, hypoxia, osmotic stress, DNA damage, heat shock, and other proteotoxic stressors. Nevertheless, little is known about the regulation and targeting of SUMO proteases during stress. To this end we have undertaken a detailed comparison of the SUMO-binding activity of the budding yeast protein Ulp1 (ScUlp1) and its ortholog in the thermotolerant yeast Kluyveromyces marxianus, KmUlp1. We find that the catalytic UD domains of both ScUlp1 and KmUlp1 show a high degree of sequence conservation, complement a ulp1Δ mutant in vivo, and process a SUMO precursor in vitro. Next, to compare the SUMO-trapping features of both SUMO proteases we produced catalytically inactive recombinant fragments of the UD domains of ScUlp1 and KmUlp1, termed ScUTAG and KmUTAG respectively. Both ScUTAG and KmUTAG were able to efficiently bind a variety of purified SUMO isoforms and bound immobilized SUMO1 with nanomolar affinity. However, KmUTAG showed a greatly enhanced ability to bind SUMO and SUMO-modified proteins in the presence of oxidative, temperature and other stressors that induce protein misfolding. We also investigated whether a SUMO-interacting motif (SIM) in the UD domain of KmULP1 that is not conserved in ScUlp1 may contribute to the SUMO-binding properties of KmUTAG. In summary, our data reveal important details about how SUMO proteases target and bind their sumoylated substrates, especially under stress conditions. We also show that the robust pan-SUMO binding features of KmUTAG can be exploited to detect and study SUMO-modified proteins in cell culture systems.

[Research Article] Fishing gear is not frequently found in archaeological sites in the southern Levant. Bone hooks were found as early as the later Epipalaeolithic period (mainly in Natufian culture sites) and continue to appear in small numbers until the Chalcolithic period, when the first copper hooks are found. But for most sites, we have scant information about fishing gear or techniques. The paucity of fishing gear in archaeological assemblages is notable and holds true for sites near the former Mediterranean Sea shore and for inland sites situated near fresh water sources. This may be attributed to preservation issues, in part, but also seems to reflect preferences in the selection of raw material for making various fishing implements. The present paper discusses a specific type of fishing gear, the notched pebbles. These implements are small pebbles with various degrees of modification - sometime including notable modification of the original pebble by flaking and sometimes only slightly modifying it by creating the two opposed notches. We will use the assemblage found at the Neolithic site of Beisamoun, in the Hula Valley, northern Israel as a test-case for discussing raw material and other preferences and long-term aspects of conventions in fresh water fishing gear.

[Report] Grooved items are usually regarded as tools used for modifying other implements made of bone, stone, plants or wood, whether referred to as shaft straighteners, smoothers, polishers or sharpening tools. They were also attributed to various symbolic meanings in Neolithic and Chalcolithic sites in the southern Levant and they were also associated with bead manufacturing process in the Neolithic. Grooved basalts found on Early Natufian (ca. 14,000 BP) living floors at el-Wad Terrace, Mount Carmel, Israel, were the subject of microscopic use-wear analysis. Here we present the preliminary results of the research, introducing our functional reconstruction based on experiments. The most outstanding result is that traces on the perimeters, attributed to shaping of the artefact, are different from the traces inside the groove which are related to the use of the artefact. We conclude that the groove is a part of the instrument, shaped before its use in order to work the abraded materials. Traces indicate that the shaping of the perimeters was done by abrasion against a very hard rock, probably using water to enhance the smoothing of the stone. Traces inside the groove indicate, as was previously assumed for these artefacts, that the groove was used as a shaft straightener. However, traces also indicate that the groove was used to abrade different types of materials which might not be related to the preparation of shafts including reeds, wood, and especially minerals such as ochre. Our preliminary results indicate the multi-functional nature of these items shedding light on their production, use and discard history.

[Report] Bedrock features are a hallmark of the Natufian (ca. 15,000-11,500 cal BP) in the southern Levant and beyond and they include a large variety of types, from deep variants to shallow ones and from narrow mortars to wide basins. They are usually interpreted as food preparation facilities, associated with Natufian intensification of cereal and acorn consumption. However, inside the shaft of one deep narrow Natufian mortar at the entrance to Raqefet Cave (Mt. Carmel, Israel), we found a set of grid-like incisions accompanied by irregular lines. This pattern is similar in the general impression and the details of execution to incised stone slabs and objects found in other Natufian sites. As in several other Natufian objects, the incised patterns were hardly visible at the time, due to their light appearance and concealed location. The engraving act and symbolic meaning of the contents were likely more important than the display of the results. Furthermore, the Raqefet mortar was incorporated in a structured complex that also included a slab pavement and a boulder mortar. Thus, the complex motif, the specific feature it was carved on (inside a deep mortar), the associated features, and the location at the entrance to a burial cave all suggest an elaborate ceremonial and symbolic system.

The discovery of a basalt vessel workshop at Tel Hazor, one of the most important Iron Age sites in the Near East, marks a turning point in our understanding of stone artifact production and distribution during the1st millennium BCE. It offers a rare opportunity to characterize ancient raw material sources, production sites, and study production, trade and distribution systems. The basalt vessel workshop, the only one of its kind in the Levant, produced large quantities of bowl preforms and production waste. To better understand the production and distribution systems behind this specialized production center, in 2011 we initiated a focused geochemical project that concentrated on the products of this unique workshop. We measured the major and trace element composition of 44 unfinished basalt vessels from the workshop and other contexts at Hazor, and can demonstrate that the majority of these objects were derived from one specific, geochemically well-constrained, basaltic rock source. Only a few bowls clearly deviate from this geochemical composition and were produced using raw material from other sources. Thus, we believe that one major quarry existed that supplied the Hazor workshop with the majority of the basaltic raw material. The products from this specific extraction site provide us with a “Hazor reference group” that can be used to test whether or not finished vessels from Hazor and contemporary sites were produced in the Hazor workshop.

Ground stone tools served in many physical and social contexts through millennia, reflecting a wide variety of functions. Although ground stone tool studies were neglected for much of early archaeology, the last few decades witnessed a notable international uptick in the way archaeologists confront this multifaceted topic. Today, with the advance of archaeology as a discipline, research into ground stone artefacts is moving into a new phase that integrates high resolution documentation with new methodological, analytical techniques, and technological approaches. These open new vistas for an array of studies and wide-ranging interpretive endeavours related to understanding ground stone tool production and use. Inspired by these diverse analytical approaches and interpretive challenges, we founded the international Association for Ground Stone Tools Research (AGSTR) in order to promote dialogue and create an optimal, inclusive arena for scholars studying various aspects of ground stone artefacts. Scholars from around the globe met for a five day conference at the University of Haifa, for the first meeting of the newly founded AGSTR. This included the presentation of 47 papers and 17 posters. The current paper serves as an introduction to this special issue of JLS, devoted to the proceedings of the founding conference of the Association for Ground Stone Tool Research, held at the University of Haifa during July 2015.

Hunter gatherers as well as farmers used a variety of lithic raw materials to shape their world, in which some were perceived as having symbolic or mythical content. While the anthropological literature demonstrates that the extraction of raw materials of special significance was often performed differently from that of other more 'ordinary' raw materials, identifying this in the archaeological record is difficult. In this paper we wish to shed new light on this topic using the Late Neolithic-Early Chalcolithic basanite bifacial tool production site of Giv‛at Kipod, Israel. In the southern Levant basalt axes and adzes have long been understood to embody greater symbolic content than the flint axes and adzes that dominate the Neolithic and Chalcolithic bifacial assemblages. By comparing the results from our excavations at the site of Giv‛at Kipod to other production and extraction sites we exhibit how the organization of production was different than that related to the more common flint extraction in the region. While at most production and extractions sites the manufacture of various types of items is documented and the presence of tools, especially ad hoc tools, indicates that a variety of activities were performed at the locale of extraction, in the case of Giv‛at Kipod the production was focused solely on the manufacture of bifacials with a marked lack of evidence of other significant activities. We attempt to provide guidelines to characterize different exploitation patterns between raw materials of varied social significance using these differences.

The beginning of metallurgy in the ancient Near East attracts much attention. The southern Levant, with the rich assemblage of copper artifacts from the Nahal Mishmar cave and the unique gold rings of the Nahal Qanah cave, is regarded as a main center of early metallurgy during the second half of the 5th millennium CalBC. However, a recently discovered copper awl from a Middle Chalcolithic burial at Tel Tsaf, Jordan Valley, Israel, suggests that cast metal technology was introduced to the region as early as the late 6th millennium CalBC. This paper examines the chemical composition of this item and reviews its context. The results indicate that it was exported from a distant source, probably in the Caucasus, and that the location where it was found is indicative of the social status of the buried individual. This rare finding indicates that metallurgy was first diffused [corrected] to the southern Levant through exchange networks and only centuries later involved local productionThis copper awl, the earliest metal artifact found in the southern Levant, indicates that the elaborate Late Chalcolithic metallurgy developed from a more ancient tradition.

Ein Qedem 2 (EQ2) is an open-air Kebaran site (Early Epipalaeolithic period, ca. 22 000 –17 500 calBP) located on the slope of Mount Carmel, Israel. The site is a part of a complex of prehistoric sites situated around the perennial spring of Ein Qedem and near the outskirts of the city of Haifa. EQ2, located near affluent sources of flint, presents ample evidence for Kebaran flint knapping. The rich flint assemblage provides valuable information concerning the microlithic industry, one of the hallmarks of the Epipalaeolithic period in this region.