91 results on '"Daniels MP"'
Search Results
2. High prevalence of respiratory ciliary dysfunction in congenital heart disease patients with heterotaxy.
- Author
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Nakhleh N, Francis R, Giese RA, Tian X, Li Y, Zariwala MA, Yagi H, Khalifa O, Kureshi S, Chatterjee B, Sabol SL, Swisher M, Connelly PS, Daniels MP, Srinivasan A, Kuehl K, Kravitz N, Burns K, Sami I, and Omran H
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- 2012
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3. Laminin induces acetylcholine receptor aggregation on cultured myotubes and enhances the receptor aggregation activity of a neuronal factor
- Author
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Vogel, Z, primary, Christian, CN, additional, Vigny, M, additional, Bauer, HC, additional, Sonderegger, P, additional, and Daniels, MP, additional
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- 1983
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4. Conditioned medium from cultures of embryonic neurons contains a high molecular weight factor which induces acetylcholine receptor aggregation on cultured myotubes
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Schaffner, AE, primary and Daniels, MP, additional
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- 1982
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5. Development of ultrastructural specializations during the formation of acetylcholine receptor aggregates on cultured myotubes
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Olek, AJ, primary, Ling, A, additional, and Daniels, MP, additional
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- 1986
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6. Loss of Drosophila i-AAA protease, dYME1L, causes abnormal mitochondria and apoptotic degeneration.
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Qi Y, Liu H, Daniels MP, Zhang G, and Xu H
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- Animals, Cell Line, Drosophila Proteins metabolism, Drosophila melanogaster, Gene Knockout Techniques, Male, Metalloendopeptidases metabolism, Mitochondria pathology, Reactive Oxygen Species metabolism, Unfolded Protein Response, Apoptosis, Drosophila Proteins genetics, Metalloendopeptidases genetics, Mitochondria enzymology, Nerve Degeneration enzymology
- Abstract
Mitochondrial AAA (ATPases Associated with diverse cellular Activities) proteases i-AAA (intermembrane space-AAA) and m-AAA (matrix-AAA) are closely related and have major roles in inner membrane protein homeostasis. Mutations of m-AAA proteases are associated with neuromuscular disorders in humans. However, the role of i-AAA in metazoans is poorly understood. We generated a deletion affecting Drosophila i-AAA, dYME1L (dYME1L(del)). Mutant flies exhibited premature aging, progressive locomotor deficiency and neurodegeneration that resemble some key features of m-AAA diseases. dYME1L(del) flies displayed elevated mitochondrial unfolded protein stress and irregular cristae. Aged dYME1L(del) flies had reduced complex I (NADH/ubiquinone oxidoreductase) activity, increased level of reactive oxygen species (ROS), severely disorganized mitochondrial membranes and increased apoptosis. Furthermore, inhibiting apoptosis by targeting dOmi (Drosophila Htra2/Omi) or DIAP1, or reducing ROS accumulation suppressed retinal degeneration. Our results suggest that i-AAA is essential for removing unfolded proteins and maintaining mitochondrial membrane architecture. Loss of i-AAA leads to the accumulation of oxidative damage and progressive deterioration of membrane integrity, which might contribute to apoptosis upon the release of proapoptotic molecules such as dOmi. Containing ROS level could be a potential strategy to manage mitochondrial AAA protease deficiency.
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- 2016
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7. Targeted disruption of PDE3B, but not PDE3A, protects murine heart from ischemia/reperfusion injury.
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Chung YW, Lagranha C, Chen Y, Sun J, Tong G, Hockman SC, Ahmad F, Esfahani SG, Bae DH, Polidovitch N, Wu J, Rhee DK, Lee BS, Gucek M, Daniels MP, Brantner CA, Backx PH, Murphy E, and Manganiello VC
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- Animals, Caveolin 3 genetics, Caveolin 3 metabolism, Connexin 43 genetics, Connexin 43 metabolism, Cyclic AMP genetics, Cyclic AMP metabolism, Cyclic AMP-Dependent Protein Kinases genetics, Cyclic AMP-Dependent Protein Kinases metabolism, Cyclic Nucleotide Phosphodiesterases, Type 3 metabolism, Mice, Mice, Knockout, Mitochondria, Heart genetics, Mitochondria, Heart metabolism, Mitochondria, Heart pathology, Mitochondrial Membrane Transport Proteins genetics, Mitochondrial Membrane Transport Proteins metabolism, Mitochondrial Membrane Transport Proteins pharmacology, Mitochondrial Permeability Transition Pore, Myocardial Infarction enzymology, Myocardial Infarction genetics, Myocardial Infarction pathology, Myocardial Infarction prevention & control, Myocardium pathology, Phosphodiesterase Inhibitors pharmacology, Quinolones pharmacology, Cyclic Nucleotide Phosphodiesterases, Type 3 deficiency, Myocardial Reperfusion Injury enzymology, Myocardial Reperfusion Injury genetics, Myocardial Reperfusion Injury pathology, Myocardial Reperfusion Injury prevention & control, Myocardium enzymology
- Abstract
Although inhibition of cyclic nucleotide phosphodiesterase type 3 (PDE3) has been reported to protect rodent heart against ischemia/reperfusion (I/R) injury, neither the specific PDE3 isoform involved nor the underlying mechanisms have been identified. Targeted disruption of PDE3 subfamily B (PDE3B), but not of PDE3 subfamily A (PDE3A), protected mouse heart from I/R injury in vivo and in vitro, with reduced infarct size and improved cardiac function. The cardioprotective effect in PDE3B(-/-) heart was reversed by blocking cAMP-dependent PKA and by paxilline, an inhibitor of mitochondrial calcium-activated K channels, the opening of which is potentiated by cAMP/PKA signaling. Compared with WT mitochondria, PDE3B(-/-) mitochondria were enriched in antiapoptotic Bcl-2, produced less reactive oxygen species, and more frequently contacted transverse tubules where PDE3B was localized with caveolin-3. Moreover, a PDE3B(-/-) mitochondrial fraction containing connexin-43 and caveolin-3 was more resistant to Ca(2+)-induced opening of the mitochondrial permeability transition pore. Proteomics analyses indicated that PDE3B(-/-) heart mitochondria fractions were enriched in buoyant ischemia-induced caveolin-3-enriched fractions (ICEFs) containing cardioprotective proteins. Accumulation of proteins into ICEFs was PKA dependent and was achieved by ischemic preconditioning or treatment of WT heart with the PDE3 inhibitor cilostamide. Taken together, these findings indicate that PDE3B deletion confers cardioprotective effects because of cAMP/PKA-induced preconditioning, which is associated with the accumulation of proteins with cardioprotective function in ICEFs. To our knowledge, our study is the first to define a role for PDE3B in cardioprotection against I/R injury and suggests PDE3B as a target for cardiovascular therapies.
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- 2015
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8. Secretion of fatty acid binding protein aP2 from adipocytes through a nonclassical pathway in response to adipocyte lipase activity.
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Ertunc ME, Sikkeland J, Fenaroli F, Griffiths G, Daniels MP, Cao H, Saatcioglu F, and Hotamisligil GS
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- 3T3-L1 Cells, Animals, Carrier Proteins genetics, Carrier Proteins metabolism, Fatty Acid-Binding Proteins genetics, Fluorescent Antibody Technique, Lipase genetics, Male, Mice, Mice, Knockout, Microscopy, Confocal, Perilipin-1, Phosphoproteins genetics, Phosphoproteins metabolism, Adipocytes metabolism, Adipose Tissue metabolism, Fatty Acid-Binding Proteins metabolism, Lipase metabolism
- Abstract
Adipocyte fatty acid binding protein 4, aP2, contributes to the pathogenesis of several common diseases including type 2 diabetes, atherosclerosis, fatty liver disease, asthma, and cancer. Although the biological functions of aP2 have classically been attributed to its intracellular action, recent studies demonstrated that aP2 acts as an adipokine to regulate systemic metabolism. However, the mechanism and regulation of aP2 secretion remain unknown. Here, we demonstrate a specific role for lipase activity in aP2 secretion from adipocytes in vitro and ex vivo. Our results show that chemical inhibition of lipase activity, genetic deficiency of adipose triglyceride lipase and, to a lesser extent, hormone-sensitive lipase blocked aP2 secretion from adipocytes. Increased lipolysis and lipid availability also contributed to aP2 release as determined in perilipin1-deficient adipose tissue explants ex vivo and upon treatment with lipids in vivo and in vitro. In addition, we identify a nonclassical route for aP2 secretion in exosome-like vesicles and show that aP2 is recruited to this pathway upon stimulation of lipolysis. Given the effect of circulating aP2 on glucose metabolism, these data support that targeting aP2 or the lipolysis-dependent secretory pathway may present novel mechanistic and translational opportunities in metabolic disease.
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- 2015
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9. Imaging flow cytometry documents incomplete resistance of human sickle F-cells to ex vivo hypoxia-induced sickling.
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Fertrin KY, van Beers EJ, Samsel L, Mendelsohn LG, Saiyed R, Nichols JS, Hepp DA, Brantner CA, Daniels MP, McCoy JP, and Kato GJ
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- Humans, Anemia, Sickle Cell immunology, Anemia, Sickle Cell therapy, Fetal Hemoglobin immunology
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- 2014
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10. Imaging flow cytometry for automated detection of hypoxia-induced erythrocyte shape change in sickle cell disease.
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van Beers EJ, Samsel L, Mendelsohn L, Saiyed R, Fertrin KY, Brantner CA, Daniels MP, Nichols J, McCoy JP, and Kato GJ
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- Anemia, Sickle Cell pathology, Automation methods, Flow Cytometry methods, Humans, Oxygen blood, Anemia, Sickle Cell blood, Cell Hypoxia physiology, Erythrocytes pathology, Erythrocytes, Abnormal pathology
- Abstract
In preclinical and early phase pharmacologic trials in sickle cell disease, the percentage of sickled erythrocytes after deoxygenation, an ex vivo functional sickling assay, has been used as a measure of a patient's disease outcome. We developed a new sickle imaging flow cytometry assay (SIFCA) and investigated its application. To perform the SIFCA, peripheral blood was diluted, deoxygenated (2% oxygen) for 2 hr, fixed, and analyzed using imaging flow cytometry. We developed a software algorithm that correctly classified investigator tagged "sickled" and "normal" erythrocyte morphology with a sensitivity of 100% and a specificity of 99.1%. The percentage of sickled cells as measured by SIFCA correlated strongly with the percentage of sickle cell anemia blood in experimentally admixed samples (R = 0.98, P ≤ 0.001), negatively with fetal hemoglobin (HbF) levels (R = -0.558, P = 0.027), negatively with pH (R = -0.688, P = 0.026), negatively with pretreatment with the antisickling agent, Aes-103 (5-hydroxymethyl-2-furfural) (R = -0.766, P = 0.002), and positively with the presence of long intracellular fibers as visualized by transmission electron microscopy (R = 0.799, P = 0.002). This study shows proof of principle that the automated, operator-independent SIFCA is associated with predictable physiologic and clinical parameters and is altered by the putative antisickling agent, Aes-103. SIFCA is a new method that may be useful in sickle cell drug development., (© 2014 Wiley Periodicals, Inc.)
- Published
- 2014
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11. In vivo microscopy reveals extensive embedding of capillaries within the sarcolemma of skeletal muscle fibers.
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Glancy B, Hsu LY, Dao L, Bakalar M, French S, Chess DJ, Taylor JL, Picard M, Aponte A, Daniels MP, Esfahani S, Cushman S, and Balaban RS
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- Animals, Biological Transport, Active physiology, Glucose Transporter Type 4 metabolism, Mice, Mice, Knockout, Microscopy, Video, Mitochondria, Muscle genetics, Capillaries metabolism, Mitochondria, Muscle metabolism, Muscle Fibers, Skeletal metabolism, Oxygen metabolism, Sarcolemma metabolism
- Abstract
Objective: To provide insight into mitochondrial function in vivo, we evaluated the 3D spatial relationship between capillaries, mitochondria, and muscle fibers in live mice., Methods: 3D volumes of in vivo murine TA muscles were imaged by MPM. Muscle fiber type, mitochondrial distribution, number of capillaries, and capillary-to-fiber contact were assessed. The role of Mb-facilitated diffusion was examined in Mb KO mice. Distribution of GLUT4 was also evaluated in the context of the capillary and mitochondrial network., Results: MPM revealed that 43.6 ± 3.3% of oxidative fiber capillaries had ≥50% of their circumference embedded in a groove in the sarcolemma, in vivo. Embedded capillaries were tightly associated with dense mitochondrial populations lateral to capillary grooves and nearly absent below the groove. Mitochondrial distribution, number of embedded capillaries, and capillary-to-fiber contact were proportional to fiber oxidative capacity and unaffected by Mb KO. GLUT4 did not preferentially localize to embedded capillaries., Conclusions: Embedding capillaries in the sarcolemma may provide a regulatory mechanism to optimize delivery of oxygen to heterogeneous groups of muscle fibers. We hypothesize that mitochondria locate to PV regions due to myofibril voids created by embedded capillaries, not to enhance the delivery of oxygen to the mitochondria., (© 2013 John Wiley & Sons Ltd.)
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- 2014
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12. Direct modulation of the outer mitochondrial membrane channel, voltage-dependent anion channel 1 (VDAC1) by cannabidiol: a novel mechanism for cannabinoid-induced cell death.
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Rimmerman N, Ben-Hail D, Porat Z, Juknat A, Kozela E, Daniels MP, Connelly PS, Leishman E, Bradshaw HB, Shoshan-Barmatz V, and Vogel Z
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- Animals, Blotting, Western, Cannabinoids pharmacology, Cell Death drug effects, Cell Line, Flow Cytometry, Mice, Microglia drug effects, Voltage-Dependent Anion Channel 1 genetics, Cannabidiol pharmacology, Voltage-Dependent Anion Channel 1 metabolism
- Abstract
Cannabidiol (CBD) is a non-psychoactive plant cannabinoid that inhibits cell proliferation and induces cell death of cancer cells and activated immune cells. It is not an agonist of the classical CB1/CB2 cannabinoid receptors and the mechanism by which it functions is unknown. Here, we studied the effects of CBD on various mitochondrial functions in BV-2 microglial cells. Our findings indicate that CBD treatment leads to a biphasic increase in intracellular calcium levels and to changes in mitochondrial function and morphology leading to cell death. Density gradient fractionation analysis by mass spectrometry and western blotting showed colocalization of CBD with protein markers of mitochondria. Single-channel recordings of the outer-mitochondrial membrane protein, the voltage-dependent anion channel 1 (VDAC1) functioning in cell energy, metabolic homeostasis and apoptosis revealed that CBD markedly decreases channel conductance. Finally, using microscale thermophoresis, we showed a direct interaction between purified fluorescently labeled VDAC1 and CBD. Thus, VDAC1 seems to serve as a novel mitochondrial target for CBD. The inhibition of VDAC1 by CBD may be responsible for the immunosuppressive and anticancer effects of CBD.
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- 2013
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13. Restricted mitochondrial protein acetylation initiates mitochondrial autophagy.
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Webster BR, Scott I, Han K, Li JH, Lu Z, Stevens MV, Malide D, Chen Y, Samsel L, Connelly PS, Daniels MP, McCoy JP Jr, Combs CA, Gucek M, and Sack MN
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- Acetylation, Animals, HEK293 Cells, HeLa Cells, Humans, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitochondria enzymology, Mitochondria genetics, Mitochondrial Proteins genetics, Nerve Tissue Proteins genetics, Nerve Tissue Proteins metabolism, Ubiquitin-Protein Ligases genetics, Ubiquitin-Protein Ligases metabolism, Autophagy, Mitochondria metabolism, Mitochondrial Proteins metabolism
- Abstract
Because nutrient-sensing nuclear and cytosolic acetylation mediates cellular autophagy, we investigated whether mitochondrial acetylation modulates mitochondrial autophagy (mitophagy). Knockdown of GCN5L1, a component of the mitochondrial acetyltransferase machinery, diminished mitochondrial protein acetylation and augmented mitochondrial enrichment of autophagy mediators. This program was disrupted by SIRT3 knockdown. Chronic GCN5L1 depletion increased mitochondrial turnover and reduced mitochondrial protein content and/or mass. In parallel, mitochondria showed blunted respiration and enhanced 'stress-resilience'. Genetic disruption of autophagy mediators Atg5 and p62 (also known as SQSTM1), as well as GCN5L1 reconstitution, abolished deacetylation-induced mitochondrial autophagy. Interestingly, this program is independent of the mitophagy E3-ligase Parkin (also known as PARK2). Taken together, these data suggest that deacetylation of mitochondrial proteins initiates mitochondrial autophagy in a canonical autophagy-mediator-dependent program and shows that modulation of this regulatory program has ameliorative mitochondrial homeostatic effects.
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- 2013
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14. Autophagy regulates endothelial cell processing, maturation and secretion of von Willebrand factor.
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Torisu T, Torisu K, Lee IH, Liu J, Malide D, Combs CA, Wu XS, Rovira II, Fergusson MM, Weigert R, Connelly PS, Daniels MP, Komatsu M, Cao L, and Finkel T
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- Autophagy-Related Protein 5, Autophagy-Related Protein 7, Exocytosis, Hemostasis, Humans, Microtubule-Associated Proteins genetics, Ubiquitin-Activating Enzymes genetics, Weibel-Palade Bodies metabolism, Autophagy, Endothelial Cells metabolism, von Willebrand Factor metabolism
- Abstract
Endothelial secretion of von Willebrand factor (VWF) from intracellular organelles known as Weibel-Palade bodies (WPBs) is required for platelet adhesion to the injured vessel wall. Here we demonstrate that WPBs are often found near or within autophagosomes and that endothelial autophagosomes contain abundant VWF protein. Pharmacological inhibitors of autophagy or knockdown of the essential autophagy genes Atg5 or Atg7 inhibits the in vitro secretion of VWF. Furthermore, although mice with endothelial-specific deletion of Atg7 have normal vessel architecture and capillary density, they exhibit impaired epinephrine-stimulated VWF release, reduced levels of high-molecular weight VWF multimers and a corresponding prolongation of bleeding times. Endothelial-specific deletion of Atg5 or pharmacological inhibition of autophagic flux results in a similar in vivo alteration of hemostasis. Thus, autophagy regulates endothelial VWF secretion, and transient pharmacological inhibition of autophagic flux may be a useful strategy to prevent thrombotic events.
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- 2013
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15. The role of agrin in synaptic development, plasticity and signaling in the central nervous system.
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Daniels MP
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- Animals, Mice, Mice, Knockout, Agrin physiology, Central Nervous System physiology, Neuronal Plasticity physiology, Signal Transduction physiology, Synapses physiology
- Abstract
Development of the neuromuscular junction (NMJ) requires secretion of specific isoforms of the proteoglycan agrin by motor neurons. Secreted agrin is widely expressed in the basal lamina of various tissues, whereas a transmembrane form is highly expressed in the brain. Expression in the brain is greatest during the period of synaptogenesis, but remains high in regions of the adult brain that show extensive synaptic plasticity. The well-established role of agrin in NMJ development and its presence in the brain elicited investigations of its possible role in synaptogenesis in the brain. Initial studies on the embryonic brain and neuronal cultures of agrin-null mice did not reveal any defects in synaptogenesis. However, subsequent studies in culture demonstrated inhibition of synaptogenesis by agrin antisense oligonucleotides or agrin siRNA. More recently, a substantial loss of excitatory synapses was found in the brains of transgenic adult mice that lacked agrin expression everywhere but in motor neurons. The mechanisms by which agrin influences synapse formation, maintenance and plasticity may include enhancement of excitatory synaptic signaling, activation of the "muscle-specific" receptor tyrosine kinase (MuSK) and positive regulation of dendritic filopodia. In this article I will review the evidence that agrin regulates synapse development, plasticity and signaling in the brain and discuss the evidence for the proposed mechanisms., (Published by Elsevier Ltd.)
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- 2012
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16. Deletion of mitochondrial associated ubiquitin fold modifier protein Ufm1 in Leishmania donovani results in loss of β-oxidation of fatty acids and blocks cell division in the amastigote stage.
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Gannavaram S, Connelly PS, Daniels MP, Duncan R, Salotra P, and Nakhasi HL
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- Cell Survival, Genetic Complementation Test, Humans, Leishmania donovani genetics, Macrophages immunology, Macrophages parasitology, Oxidation-Reduction, Protozoan Proteins genetics, Cell Division, Fatty Acids metabolism, Gene Deletion, Leishmania donovani enzymology, Leishmania donovani physiology, Protozoan Proteins metabolism, Ubiquitin metabolism
- Abstract
Recently, we described the existence of the ubiquitin fold modifier 1 (Ufm1) and its conjugation pathway in Leishmania donovani. We demonstrated the conjugation of Ufm1 to proteins such as mitochondrial trifunctional protein (MTP) that catalyses β-oxidation of fatty acids in L. donovani. To elucidate the biological roles of the Ufm1-mediated modifications, we made an L. donovani Ufm1 null mutant (Ufm1(-/-)). Loss of Ufm1 and consequently absence of Ufm1 conjugation with MTP resulted in diminished acetyl-CoA, the end-product of the β-oxidation in the Ufm1(-/-) amastigote stage. The Ufm1(-/-) mutants showed reduced survival in the amastigote stage in vitro and ex vivo in human macrophages. This survival was restored by re-expression of wild-type Ufm1 with concomitant induction of acetyl-CoA but not by re-expressing the non-conjugatable Ufm1, indicating the essential nature of Ufm1 conjugation and β-oxidation. Both cell cycle analysis and ultrastructural studies of Ufm1(-/-) parasites confirmed the role of Ufm1 in amastigote growth. The defect in vitro growth of amastigotes in human macrophages was further substantiated by reduced survival. Therefore, these studies suggest the importance of Ufm1 in Leishmania pathogenesis with larger impact on other organisms and further provide an opportunity to test Ufm1(-/-) parasites as drug and vaccine targets., (Published 2012. This article is a U.S. Government work and is in the public domain in the USA.)
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- 2012
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17. Mouse models of MYH9-related disease: mutations in nonmuscle myosin II-A.
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Zhang Y, Conti MA, Malide D, Dong F, Wang A, Shmist YA, Liu C, Zerfas P, Daniels MP, Chan CC, Kozin E, Kachar B, Kelley MJ, Kopp JB, and Adelstein RS
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- Animals, Cataract metabolism, Cataract pathology, Female, Fluorescent Antibody Technique, Genes, Lethal, Hearing Loss metabolism, Hearing Loss pathology, Heterozygote, Homozygote, Humans, Immunoblotting, Kidney Diseases metabolism, Kidney Diseases pathology, Male, Megakaryocytes metabolism, Mice, Mice, Transgenic, Myosin Heavy Chains, Platelet Count, Thrombocytopenia metabolism, Thrombocytopenia pathology, Cataract etiology, Disease Models, Animal, Hearing Loss etiology, Kidney Diseases etiology, Megakaryocytes pathology, Mutation genetics, Nonmuscle Myosin Type IIA physiology, Thrombocytopenia etiology
- Abstract
We have generated 3 mouse lines, each with a different mutation in the nonmuscle myosin II-A gene, Myh9 (R702C, D1424N, and E1841K). Each line develops MYH9-related disease similar to that found in human patients. R702C mutant human cDNA fused with green fluorescent protein was introduced into the first coding exon of Myh9, and D1424N and E1841K mutations were introduced directly into the corresponding exons. Homozygous R702C mice die at embryonic day 10.5-11.5, whereas homozygous D1424N and E1841K mice are viable. All heterozygous and homozygous mutant mice show macrothrombocytopenia with prolonged bleeding times, a defect in clot retraction, and increased extramedullary megakaryocytes. Studies of cultured megakaryocytes and live-cell imaging of megakaryocytes in the BM show that heterozygous R702C megakaryocytes form fewer and shorter proplatelets with less branching and larger buds. The results indicate that disrupted proplatelet formation contributes to the macrothrombocytopenia in mice and most probably in humans. We also observed premature cataract formation, kidney abnormalities, including albuminuria, focal segmental glomerulosclerosis and progressive kidney disease, and mild hearing loss. Our results show that heterozygous mice with mutations in the myosin motor or filament-forming domain manifest similar hematologic, eye, and kidney phenotypes to humans with MYH9-related disease.
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- 2012
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18. Actin cross-linking proteins cortexillin I and II are required for cAMP signaling during Dictyostelium chemotaxis and development.
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Shu S, Liu X, Kriebel PW, Daniels MP, and Korn ED
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- Bacterial Proteins biosynthesis, Bacterial Proteins genetics, Chemotaxis genetics, Cyclic AMP, Dictyostelium genetics, Dictyostelium growth & development, Gene Deletion, Gene Knockout Techniques, Luminescent Proteins biosynthesis, Luminescent Proteins genetics, Microfilament Proteins genetics, Protozoan Proteins genetics, Receptors, Cyclic AMP biosynthesis, Receptors, Cyclic AMP genetics, Signal Transduction, Actins metabolism, Chemotaxis physiology, Dictyostelium physiology, Microfilament Proteins metabolism, Protozoan Proteins metabolism
- Abstract
Starvation induces Dictyostelium amoebae to secrete cAMP, toward which other amoebae stream, forming multicellular mounds that differentiate and develop into fruiting bodies containing spores. We find that the double deletion of cortexillin (ctx) I and II alters the actin cytoskeleton and substantially inhibits all molecular responses to extracellular cAMP. Synthesis of cAMP receptor and adenylyl cyclase A (ACA) is inhibited, and activation of ACA, RasC, and RasG, phosphorylation of extracellular signal regulated kinase 2, activation of TORC2, and stimulation of actin polymerization and myosin assembly are greatly reduced. As a consequence, cell streaming and development are completely blocked. Expression of ACA-yellow fluorescent protein in the ctxI/ctxII-null cells significantly rescues the wild-type phenotype, indicating that the primary chemotaxis and development defect is the inhibition of ACA synthesis and cAMP production. These results demonstrate the critical importance of a properly organized actin cytoskeleton for cAMP-signaling pathways, chemotaxis, and development in Dictyostelium.
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- 2012
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19. Imaging the post-fusion release and capture of a vesicle membrane protein.
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Sochacki KA, Larson BT, Sengupta DC, Daniels MP, Shtengel G, Hess HF, and Taraska JW
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- Animals, Cell Membrane physiology, Cell Membrane ultrastructure, Clathrin physiology, Clathrin ultrastructure, Endocytosis physiology, Exocytosis physiology, Membrane Proteins ultrastructure, Microscopy, Electron, Microscopy, Interference methods, PC12 Cells physiology, Rats, Synaptic Vesicles physiology, Synaptic Vesicles ultrastructure, Vesicular Acetylcholine Transport Proteins ultrastructure, Membrane Fusion physiology, Membrane Proteins physiology, Vesicular Acetylcholine Transport Proteins physiology
- Abstract
The molecular mechanism responsible for capturing, sorting and retrieving vesicle membrane proteins following triggered exocytosis is not understood. Here we image the post-fusion release and then capture of a vesicle membrane protein, the vesicular acetylcholine transporter, from single vesicles in living neuroendocrine cells. We combine these measurements with super-resolution interferometric photo-activation localization microscopy and electron microscopy, and modelling to map the nanometer-scale topography and architecture of the structures responsible for the transporter's capture following exocytosis. We show that after exocytosis, the transporter rapidly diffuses into the plasma membrane, but most travels only a short distance before it is locally captured over a dense network of membrane-resident clathrin-coated structures. We propose that the extreme density of these structures acts as a short-range diffusion trap. They quickly sequester diffusing vesicle material and limit its spread across the membrane. This system could provide a means for clathrin-mediated endocytosis to quickly recycle vesicle proteins in highly excitable cells.
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- 2012
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20. Disruption of caveolae blocks ischemic preconditioning-mediated S-nitrosylation of mitochondrial proteins.
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Sun J, Kohr MJ, Nguyen T, Aponte AM, Connelly PS, Esfahani SG, Gucek M, Daniels MP, Steenbergen C, and Murphy E
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- Animals, Caveolin 3 metabolism, Enzyme Activation drug effects, Heart drug effects, Male, Mice, Mice, Inbred C57BL, Mitochondria metabolism, Myocardium metabolism, Nitric Oxide Synthase Type III metabolism, Oxidation-Reduction, Phosphorylation drug effects, Protein Binding drug effects, Proto-Oncogene Proteins c-akt metabolism, Sarcolemma drug effects, Sarcolemma metabolism, Signal Transduction drug effects, beta-Cyclodextrins pharmacology, Caveolae metabolism, Ischemic Preconditioning, Myocardial, Mitochondrial Proteins metabolism, Nitric Oxide metabolism
- Abstract
Aims: Nitric oxide (NO) and protein S-nitrosylation (SNO) play important roles in ischemic preconditioning (IPC)-induced cardioprotection. Mitochondria are key regulators of preconditioning, and most proteins showing an increase in SNO with IPC are mitochondrial. The aim of this study was to address how IPC transduces NO/SNO signaling to mitochondria in the heart., Results: In this study using Langendorff perfused mouse hearts, we found that IPC-induced cardioprotection was blocked by treatment with either N-nitro-L-arginine methyl ester (L-NAME, a constitutive NO synthase inhibitor), ascorbic acid (a reducing agent to decompose SNO), or methyl-?-cyclodextrin (M?CD, a cholesterol sequestering agent to disrupt caveolae). IPC not only activated AKT/eNOS signaling but also led to translocation of eNOS to mitochondria. M?CD treatment disrupted caveolar structure, leading to dissociation of eNOS from caveolin-3 and blockade of IPC-induced activation of the AKT/eNOS signaling pathway. A significant increase in mitochondrial SNO was found in IPC hearts compared to perfusion control, and the disruption of caveolae by M?CD treatment not only abolished IPC-induced cardioprotection, but also blocked the IPC-induced increase in SNO., Innovation: These results provide mechanistic insight into how caveolae/eNOS/NO/SNO signaling mediates cardioprotection induced by IPC., Conclusion: Altogether these results suggest that caveolae transduce eNOS/NO/SNO cardioprotective signaling in the heart.
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- 2012
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21. A cytosolic phospholipase A2-initiated lipid mediator pathway induces autophagy in macrophages.
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Qi HY, Daniels MP, Liu Y, Chen LY, Alsaaty S, Levine SJ, and Shelhamer JH
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- Animals, Cell Line, Cells, Cultured, Eicosanoids physiology, Humans, Inflammation Mediators physiology, Macrophages cytology, Mice, Monocytes cytology, Monocytes enzymology, Monocytes immunology, Autophagy immunology, Lipid Metabolism immunology, Macrophages enzymology, Macrophages immunology, Phospholipases A2, Cytosolic physiology, Signal Transduction immunology
- Abstract
Autophagy delivers cytoplasmic constituents to autophagosomes and is involved in innate and adaptive immunity. Cytosolic phospholipase (cPLA(2))-initiated proinflammatory lipid mediator pathways play a critical role in host defense and inflammation. The crosstalk between the two pathways remains unclear. In this study, we report that cPLA(2) and its metabolite lipid mediators induced autophagy in the RAW246.7 macrophage cell line and in primary monocytes. IFN-γ-triggered autophagy involves activation of cPLA(2). Cysteinyl leukotrienes D(4) and E(4) and PGD(2) also induced these effects. The autophagy is independent of changes in mTOR or autophagic flux. cPLA(2) and lipid mediator-induced autophagy is ATG5 dependent. These data suggest that lipid mediators play a role in the regulation of autophagy, demonstrating a connection between the two seemingly separate innate immune responses, induction of autophagy and lipid mediator generation.
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- 2011
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22. Expression of Y53A-actin in Dictyostelium disrupts the cytoskeleton and inhibits intracellular and intercellular chemotactic signaling.
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Shu S, Liu X, Kriebel PW, Hong MS, Daniels MP, Parent CA, and Korn ED
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- Actins chemistry, Actins metabolism, Adenylyl Cyclases metabolism, Amino Acid Sequence, Cell Adhesion, Cell Line, Dictyostelium genetics, Dictyostelium growth & development, Dictyostelium physiology, Mutation, Phosphorylation, Receptors, Cyclic AMP metabolism, Stress, Physiological genetics, Transport Vesicles metabolism, Tyrosine metabolism, Actins genetics, Chemotaxis genetics, Cytoskeleton metabolism, Dictyostelium cytology, Extracellular Space metabolism, Gene Expression Regulation, Signal Transduction genetics
- Abstract
We showed previously that phosphorylation of Tyr(53), or its mutation to Ala, inhibits actin polymerization in vitro with formation of aggregates of short filaments, and that expression of Y53A-actin in Dictyostelium blocks differentiation and development at the mound stage (Liu, X., Shu, S., Hong, M. S., Levine, R. L., and Korn, E. D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 13694-13699; Liu, X., Shu, S., Hong, M. S., Yu, B., and Korn, E. D. (2010) J. Biol. Chem. 285, 9729-9739). We now show that expression of Y53A-actin, which does not affect cell growth, phagocytosis, or pinocytosis, inhibits the formation of head-to-tail cell streams during cAMP-induced aggregation, although individual amoebae chemotax normally. We show that expression of Y53A-actin causes a 50% reduction of cell surface cAMP receptors, and inhibits cAMP-induced increases in adenylyl cyclase A activity, phosphorylation of ERK2, and actin polymerization. Trafficking of vesicles containing adenylyl cyclase A to the rear of the cell and secretion of the ACA vesicles are also inhibited. The actin cytoskeleton of cells expressing Y53A-actin is characterized by numerous short filaments, and bundled and aggregated filaments similar to the structures formed by copolymerization of purified Y53A-actin and wild-type actin in vitro. This disorganized actin cytoskeleton may be responsible for the inhibition of intracellular and intercellular cAMP signaling in cells expressing F-Y53A-actin.
- Published
- 2010
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23. Induction of filopodia-like protrusions by transmembrane agrin: role of agrin glycosaminoglycan chains and Rho-family GTPases.
- Author
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Lin L, McCroskery S, Ross JM, Chak Y, Neuhuber B, and Daniels MP
- Subjects
- Agrin chemistry, Animals, Blotting, Western, COS Cells, Cell Line, Cell Membrane chemistry, Chlorocebus aethiops, Enzyme-Linked Immunosorbent Assay, Genes, gag genetics, Glycosaminoglycans chemistry, Immunohistochemistry, Mutation genetics, Neurons metabolism, Rats, rho GTP-Binding Proteins chemistry, Agrin metabolism, Glycosaminoglycans metabolism, Pseudopodia metabolism, rho GTP-Binding Proteins metabolism
- Abstract
Filopodia sense the extracellular environment and direct movement in many cell types, including neurons. Recent reports suggest that the transmembrane form of the widely expressed proteoglycan agrin (TM-agrin) regulates formation and stability of neuronal filopodia. In order to elucidate the mechanism by which TM-agrin regulates filopodia, we investigated the role of agrin's glycosaminoglycan (GAG) chains in the induction of filopodia formation by TM-agrin over-expression in hippocampal neurons, and in the induction of filopodia-like processes in COS7 cells. Deletion of the GAG chains of TM-agrin sharply reduced formation of filopodia-like branched retraction fibers (BRFs) in COS7 cells, with deletion of the heparan sulfate GAG chains being most effective, and eliminated filopodia induction in hippocampal neurons. GAG chain deletion also reduced the activation of Cdc42 and Rac1 resulting from TM-agrin over-expression. Moreover, dominant-negative Cdc42 and Rac1 inhibited BRF formation. Lastly, over-expression of TM-agrin increased the adhesiveness of COS7 cells and this increase was reduced by deletion of the GAG chains. Our results suggest that TM-agrin regulates actin-based protrusions in large part through interaction of its GAG chains with extracellular or transmembrane proteins, leading to the activation of Cdc42 and Rac1.
- Published
- 2010
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24. Oxidative stress causes reversible changes in mitochondrial permeability and structure.
- Author
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Cole NB, Daniels MP, Levine RL, and Kim G
- Subjects
- Animals, Biological Transport, Active, Cell Line, Glucose Oxidase metabolism, Glucose Oxidase pharmacology, Methionine Sulfoxide Reductases genetics, Methionine Sulfoxide Reductases metabolism, Mice, Microscopy, Electron, Transmission, Microscopy, Fluorescence, Mitochondria drug effects, Mitochondrial Membranes metabolism, Permeability, Reactive Oxygen Species metabolism, Recombinant Proteins genetics, Recombinant Proteins metabolism, Mitochondria metabolism, Mitochondria ultrastructure, Oxidative Stress
- Abstract
Mitochondria are a primary source as well a principal target of reactive oxygen species within cells. Using immunofluorescence microscopy, we have found that a number of mitochondrial matrix proteins are normally undetectable in formaldehyde-fixed cells permeabilized with the cholesterol-binding detergent saponin. However, exogenous or endogenous oxidative stress applied prior to fixation altered the permeability of mitochondria, rendering these matrix proteins accessible to antibodies. Electron microscopy revealed a loss of matrix density and disorganization of inner membrane cristae upon oxidative stress. Notably, the changes in permeability and in structure were rapidly reversed when the oxidative stress was relieved. The ability of reactive oxygen species to reversibly alter the permeability of the mitochondrial membrane provides a potential mechanism for communication within the cell such as between nucleus and mitochondria., (Published by Elsevier Inc.)
- Published
- 2010
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25. Transmembrane agrin regulates dendritic filopodia and synapse formation in mature hippocampal neuron cultures.
- Author
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McCroskery S, Bailey A, Lin L, and Daniels MP
- Subjects
- Agrin genetics, Animals, Cell Differentiation physiology, Cell Membrane ultrastructure, Cells, Cultured, Dendrites ultrastructure, Down-Regulation genetics, Genetic Vectors pharmacology, Hippocampus growth & development, Hippocampus ultrastructure, Neural Pathways metabolism, Neural Pathways ultrastructure, Neurogenesis physiology, Neuronal Plasticity physiology, Presynaptic Terminals metabolism, Presynaptic Terminals ultrastructure, Pseudopodia ultrastructure, Rats, Synapses ultrastructure, Synaptic Membranes metabolism, Synaptic Membranes ultrastructure, Agrin metabolism, Cell Membrane metabolism, Dendrites metabolism, Hippocampus metabolism, Pseudopodia metabolism, Synapses metabolism
- Abstract
The transmembrane isoform of agrin (Tm-agrin) is the predominant form expressed in the brain but its putative roles in brain development are not well understood. Recent reports have implicated Tm-agrin in the formation and stabilization of filopodia on neurites of immature central and peripheral neurons in culture. In maturing central neurons, dendritic filopodia are believed to facilitate synapse formation. In the present study we have investigated the role of Tm-agrin in regulation of dendritic filopodia and synaptogenesis in maturing cultures of rat hippocampal neurons. We did this by infecting the neurons with an RNAi lentivirus to deplete endogenous agrin during the developmental period when filopodia density on the dendritic arbor was high, and synapse formation was rapid. We found that dendritic filopodia density was markedly reduced, as was synapse density along dendrites. Moreover, synapse formation was more sharply reduced on dendrites of infected neurons contacted by uninfected axons than on uninfected dendrites contacted by infected axons. The results are consistent with a physiological role for Tm-agrin in the maturation of hippocampal neurons involving positive regulation of dendritic filopodia and consequent promotion of synaptogenesis, but also suggest a role for axonal agrin in synaptogenesis.
- Published
- 2009
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26. Mitochondrial dysfunction and oxidative stress mediate the physiological impairment induced by the disruption of autophagy.
- Author
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Wu JJ, Quijano C, Chen E, Liu H, Cao L, Fergusson MM, Rovira II, Gutkind S, Daniels MP, Komatsu M, and Finkel T
- Subjects
- Animals, Autophagy-Related Protein 7, Gene Expression Regulation physiology, Glucose metabolism, Insulin Resistance, Insulin-Secreting Cells metabolism, Male, Mice, Mice, Knockout, Microtubule-Associated Proteins genetics, Microtubule-Associated Proteins metabolism, Autophagy physiology, Mitochondria physiology, Oxidative Stress physiology
- Abstract
Impaired or deficient autophagy is believed to cause or contribute to aging, as well as a number of age-related pathologies. The exact mechanism through which alterations in autophagy induce these various pathologies is not well understood. Here we describe the creation of two in vivo mouse models that allow for the characterization of the alteration in mitochondrial function and the contribution of the corresponding oxidative stress following deletion of Atg7. Using these models we demonstrate that isolated mitochondria obtained from Atg7(-/-) skeletal muscle exhibit a significant defect in mitochondrial respiration. We further show that cells derived from Atg7(-/-) mice have an altered metabolic profile characterized by decreased resting mitochondrial oxygen consumption and a compensatory increase in basal glycolytic rates. Atg7(-/-)cells also exhibit evidence for increased steady state levels of reactive oxygen species. The observed mitochondrial dysfunction and oxidative stress is also evident in a mouse model where Atg7 is deleted within the pancreatic beta cell. In this model, the simple administration of an antioxidant can significantly ameliorate the physiological impairment in glucose-stimulated insulin secretion. Taken together, these results demonstrate the potential role of mitochondrial dysfunction and oxidative stress in autophagy related pathology.
- Published
- 2009
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27. Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
- Author
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Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, and Levine SJ
- Subjects
- Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Humans, Lipoproteins, IDL blood, Lipoproteins, VLDL blood, Mass Spectrometry, Protein Array Analysis, Exosomes metabolism, PPAR gamma blood, Proteomics, Serum metabolism
- Abstract
Exosomes are nanovesicles that are released from cells as a mechanism of cell-free intercellular communication. Only a limited number of proteins have been identified from the plasma exosome proteome. Here, we developed a multi-step fractionation scheme incorporating gel exclusion chromatography, rate zonal centrifugation through continuous sucrose gradients, and high-speed centrifugation to purify exosomes from human plasma. Exosome-associated proteins were separated by SDS-PAGE and 66 proteins were identified by LC-MS/MS, which included both cellular and extracellular proteins. Furthermore, we identified and characterized peroxisome proliferator-activated receptor-gamma (PPARgamma), a nuclear receptor that regulates adipocyte differentiation and proliferation, as well as immune and inflammatory cell functions, as a novel component of plasma-derived exosomes. Given the important role of exosomes as intercellular messengers, the discovery of PPARgamma as a component of human plasma exosomes identifies a potential new pathway for the paracrine transfer of nuclear receptors.
- Published
- 2009
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28. Autophagic dysfunction in mucolipidosis type IV patients.
- Author
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Vergarajauregui S, Connelly PS, Daniels MP, and Puertollano R
- Subjects
- Autophagy, Endosomes metabolism, Fibroblasts cytology, Fibroblasts metabolism, Humans, Lysosomes metabolism, Receptors, Platelet-Derived Growth Factor metabolism, TRPM Cation Channels metabolism, Transient Receptor Potential Channels, Mucolipidoses physiopathology
- Abstract
Mutations in Mucolipin 1 (MCOLN1) have been linked to mucolipidosis type IV (MLIV), a lysosomal storage disease characterized by several neurological and ophthalmological abnormalities. It has been proposed that MCOLN1 might regulate transport of membrane components in the late endosomal-lysosomal pathway; however, the mechanisms by which defects of MCOLN1 function result in mental and psychomotor retardation remain largely unknown. In this study, we show constitutive activation of autophagy in fibroblasts obtained from MLIV patients. Accumulation of autophagosomes in MLIV cells was due to the increased de novo autophagosome formation and to delayed fusion of autophagosomes with late endosomes/lysosomes. Impairment of the autophagic pathway led to increased levels and aggregation of p62, suggesting that abnormal accumulation of ubiquitin proteins may contribute to the neurodegeneration observed in MLIV patients. In addition, we found that delivery of platelet-derived growth factor receptor to lysosomes is delayed in MCOLN1-deficient cells, suggesting that MCOLN1 is necessary for efficient fusion of both autophagosomes and late endosomes with lysosomes. Our data are in agreement with recent evidence showing that autophagic defects may be a common characteristic of many neurodegenerative disorders.
- Published
- 2008
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29. Krp1 (Sarcosin) promotes lateral fusion of myofibril assembly intermediates in cultured mouse cardiomyocytes.
- Author
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Greenberg CC, Connelly PS, Daniels MP, and Horowits R
- Subjects
- Animals, Cells, Cultured, Cytoskeletal Proteins genetics, Heart embryology, Mice, Microscopy, Electron, Myofibrils ultrastructure, RNA, Small Interfering pharmacology, Cytoskeletal Proteins physiology, Muscle Proteins physiology, Myocytes, Cardiac cytology, Myofibrils metabolism
- Abstract
Krp1, also called sarcosin, is a cardiac and skeletal muscle kelch repeat protein hypothesized to promote the assembly of myofibrils, the contractile organelles of striated muscles, through interaction with N-RAP and actin. To elucidate its role, endogenous Krp1 was studied in primary embryonic mouse cardiomyocytes. While immunofluorescence showed punctate Krp1 distribution throughout the cell, detergent extraction revealed a significant pool of Krp1 associated with cytoskeletal elements. Reduction of Krp1 expression with siRNA resulted in specific inhibition of myofibril accumulation with no effect on cell spreading. Immunostaining analysis and electron microscopy revealed that cardiomyocytes lacking Krp1 contained sarcomeric proteins with longitudinal periodicities similar to mature myofibrils, but fibrils remained thin and separated. These thin myofibrils were degraded by a scission mechanism distinct from the myofibril disassembly pathway observed during cell division in the developing heart. The data are consistent with a model in which Krp1 promotes lateral fusion of adjacent thin fibrils into mature, wide myofibrils and contribute insight into mechanisms of myofibrillogenesis and disassembly.
- Published
- 2008
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30. Heterotaxy and complex structural heart defects in a mutant mouse model of primary ciliary dyskinesia.
- Author
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Tan SY, Rosenthal J, Zhao XQ, Francis RJ, Chatterjee B, Sabol SL, Linask KL, Bracero L, Connelly PS, Daniels MP, Yu Q, Omran H, Leatherbury L, and Lo CW
- Subjects
- Animals, Cilia genetics, Cilia ultrastructure, Ciliary Motility Disorders genetics, Ciliary Motility Disorders physiopathology, Disease Models, Animal, Genes, Recessive, Heart Defects, Congenital genetics, Heart Defects, Congenital pathology, Humans, Lung physiopathology, Lung ultrastructure, Mice, Mice, Mutant Strains, Myocardium ultrastructure, Situs Inversus genetics, Situs Inversus physiopathology, Vena Cava, Inferior physiopathology, Vena Cava, Inferior ultrastructure, Ciliary Motility Disorders pathology, Dyneins genetics, Heart Defects, Congenital ultrastructure, Mutation, Situs Inversus ultrastructure
- Abstract
Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder associated with ciliary defects and situs inversus totalis, the complete mirror image reversal of internal organ situs (positioning). A variable incidence of heterotaxy, or irregular organ situs, also has been reported in PCD patients, but it is not known whether this is elicited by the PCD-causing genetic lesion. We studied a mouse model of PCD with a recessive mutation in Dnahc5, a dynein gene commonly mutated in PCD. Analysis of homozygous mutant embryos from 18 litters yielded 25% with normal organ situs, 35% with situs inversus totalis, and 40% with heterotaxy. Embryos with heterotaxy had complex structural heart defects that included discordant atrioventricular and ventricular outflow situs and atrial/pulmonary isomerisms. Variable combinations of a distinct set of cardiovascular anomalies were observed, including superior-inferior ventricles, great artery alignment defects, and interrupted inferior vena cava with azygos continuation. The surprisingly high incidence of heterotaxy led us to evaluate the diagnosis of PCD. PCD was confirmed by EM, which revealed missing outer dynein arms in the respiratory cilia. Ciliary dyskinesia was observed by videomicroscopy. These findings show that Dnahc5 is required for the specification of left-right asymmetry and suggest that the PCD-causing Dnahc5 mutation may also be associated with heterotaxy.
- Published
- 2007
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31. Long-term fate of neural precursor cells following transplantation into developing and adult CNS.
- Author
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Lepore AC, Neuhuber B, Connors TM, Han SS, Liu Y, Daniels MP, Rao MS, and Fischer I
- Subjects
- Age Factors, Animals, Animals, Newborn, Cells, Cultured, Central Nervous System cytology, Central Nervous System surgery, Embryo, Mammalian, Female, Gangliosides metabolism, Graft Survival drug effects, Immunohistochemistry methods, Immunosuppressive Agents pharmacology, Microscopy, Electron, Transmission methods, Nerve Tissue Proteins metabolism, Neural Cell Adhesion Molecules metabolism, Neuroglia physiology, Neuroglia ultrastructure, Neurons ultrastructure, Pregnancy, Rats, Rats, Inbred F344, Reverse Transcriptase Polymerase Chain Reaction methods, Time Factors, Cell Differentiation physiology, Central Nervous System physiology, Neurons physiology, Stem Cell Transplantation methods, Stem Cells physiology
- Abstract
Successful strategies for transplantation of neural precursor cells for replacement of lost or dysfunctional CNS cells require long-term survival of grafted cells and integration with the host system, potentially for the life of the recipient. It is also important to demonstrate that transplants do not result in adverse outcomes. Few studies have examined the long-term properties of transplanted neural precursor cells in the CNS, particularly in non-neurogenic regions of the adult. The aim of the present study was to extensively characterize the fate of defined populations of neural precursor cells following transplantation into the developing and adult CNS (brain and spinal cord) for up to 15 months, including integration of graft-derived neurons with the host. Specifically, we employed neuronal-restricted precursors and glial-restricted precursors, which represent neural precursor cells with lineage restrictions for neuronal and glial fate, respectively. Transplanted cells were prepared from embryonic day-13.5 fetal spinal cord of transgenic donor rats that express the marker gene human placental alkaline phosphatase to achieve stable and reliable graft tracking. We found that in both developing and adult CNS grafted cells showed long-term survival, morphological maturation, extensive distribution and differentiation into all mature CNS cell types (neurons, astrocytes and oligodendrocytes). Graft-derived neurons also formed synapses, as identified by electron microscopy, suggesting that transplanted neural precursor cells integrated with adult CNS. Furthermore, grafts did not result in any apparent deleterious outcomes. We did not detect tumor formation, cells did not localize to unwanted locations and no pronounced immune response was present at the graft sites. The long-term stability of neuronal-restricted precursors and glial-restricted precursors and the lack of adverse effects suggest that transplantation of lineage-restricted neural precursor cells can serve as an effective and safe replacement therapy for CNS injury and degeneration.
- Published
- 2006
- Full Text
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32. Transmembrane agrin regulates filopodia in rat hippocampal neurons in culture.
- Author
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McCroskery S, Chaudhry A, Lin L, and Daniels MP
- Subjects
- Agrin genetics, Animals, Cell Line, Cells, Cultured, Enzyme Activation, Neurons cytology, Pseudopodia ultrastructure, RNA, Small Interfering genetics, RNA, Small Interfering metabolism, Rats, Transfection, cdc42 GTP-Binding Protein genetics, cdc42 GTP-Binding Protein metabolism, Agrin metabolism, Cell Membrane metabolism, Hippocampus cytology, Neurons metabolism, Neurons ultrastructure, Pseudopodia metabolism
- Abstract
Filopodia mediate axon guidance, neurite branching and synapse formation, but the membrane molecules that regulate neuronal filopodia in response to extracellular cues are largely unknown. The transmembrane isoform of the proteoglycan agrin, expressed predominantly in the CNS, may regulate neurite outgrowth, synapse formation and excitatory signaling. Here we demonstrate that agrin positively regulates neuronal filopodia. Over-expression of TM-agrin caused the formation of excess filopodia on neurites of hippocampal neurons cultured 1-6 days. Conversely, suppression of agrin expression by siRNA reduced the number of filopodia. Time lapse analysis indicated that endogenous TM-agrin regulates filopodia by increasing their stability and initiation. The N-terminal half of agrin was necessary for induction of filopodia, and over-expression of TM-agrin in a neuronal cell line increased Cdc42 activation, suggesting a role for Cdc42 downstream of agrin. By positively regulating filopodia in developing neurons, TM-agrin may influence the pattern of neurite outgrowth and synapse formation.
- Published
- 2006
- Full Text
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33. Calcineurin localization in skeletal muscle offers insights into potential new targets.
- Author
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Torgan CE and Daniels MP
- Subjects
- Animals, Cell Nucleolus enzymology, Cells, Cultured, Chlorocebus aethiops, Cytoplasm enzymology, Mice, Muscle Fibers, Skeletal enzymology, Muscle, Skeletal embryology, Muscle, Skeletal ultrastructure, Rats, Ryanodine Receptor Calcium Release Channel metabolism, Calcineurin metabolism, Muscle, Skeletal enzymology
- Abstract
The Ca(2+)/calmodulin-activated protein phosphatase, calcineurin, is believed to regulate the development and function of skeletal and cardiac muscle. Striated muscle contains many calcineurin substrates, a few of which have been colocalized or found in molecular complexes with calcineurin. We examined the subcellular distribution of calcineurin in developing rat skeletal muscle cells and adult mouse skeletal muscle fibers by immunofluorescence microscopy. We found low levels of calcineurin immunoreactivity in the cytoplasm of myoblasts and higher levels in cytoplasmic vesicles of myotubes. Most of these vesicles were not immunoreactive for ryanodine receptors and, those that were, represented a small fraction of nascent triad junctions. In adult myofibers, calcineurin was largely associated with triads. Weaker calcineurin immunoreactivity occurred in the sarcoplasmic reticulum at the level of the M line. Unexpectedly, we found tiny clusters of calcineurin associated with nucleoli of developing myofiber nuclei. There were one to three clusters per nucleolus, either within or at the edges of fibrillar centers where ribosomal genes are transcribed. This suggests a role for calcineurin in regulating ribosome synthesis. Our findings suggest a variety of potential new targets and pathways through which calcineurin could regulate skeletal muscle development and plasticity and underscore the importance of spatial specificity in this regulation.
- Published
- 2006
- Full Text
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34. Targeting of recombinant agrin to axonal growth cones.
- Author
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Neuhuber B and Daniels MP
- Subjects
- Agrin genetics, Animals, Cells, Cultured, Chickens, Mutation, Rats, Recombinant Proteins genetics, Agrin biosynthesis, Axons metabolism, Gene Targeting methods, Growth Cones metabolism, Recombinant Proteins biosynthesis
- Abstract
Targeting of proteins to specific subcellular locations within pre- and postsynaptic neurons is essential for synapse formation. The heparan sulfate proteoglycan agrin orchestrates postsynaptic differentiation of the neuromuscular junction and may be involved in synaptic development and signaling in the central nervous system (CNS). Agrin is expressed as transmembrane and secretory isoforms with distinct N-termini. We examined the distribution of recombinant agrin in cultured motor and hippocampal neurons by transfection with agrin-GFP constructs. Immunostaining revealed a vesicular transport compartment within all neurites. Plasma membrane insertion and secretion of recombinant agrin were targeted to axonal growth cones of motor neurons; transmembrane agrin-GFP was targeted predominantly to axons and axonal growth cones in hippocampal neurons. We used agrin deletion mutants to show that axonal targeting of agrin depends on multiple domains that function in an additive fashion, including the very N-terminal portions and the C-terminal half of the molecule.
- Published
- 2003
- Full Text
- View/download PDF
35. High efficiency transfection of primary skeletal muscle cells with lipid-based reagents.
- Author
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Neuhuber B, Huang DI, Daniels MP, and Torgan CE
- Subjects
- Animals, Cells, Cultured, Chloramphenicol O-Acetyltransferase genetics, DNA genetics, Feasibility Studies, Gene Expression drug effects, Genes, Reporter, Genetic Vectors, Green Fluorescent Proteins, Luminescent Proteins genetics, Muscle, Skeletal cytology, Muscle, Skeletal metabolism, Rats, beta-Galactosidase genetics, DNA metabolism, Indicators and Reagents pharmacology, Lipids pharmacology, Muscle, Skeletal drug effects, Transfection methods
- Abstract
Lipofection is a convenient method for gene transfer into muscle cells but reportedly is inefficient. We tested the efficacy of commercially available lipid-based and polyamine transfection reagents. Primary rat skeletal muscle cell cultures were transfected at three stages of development and assayed after fusion. Efficiency reached 30% during the proliferation stage and up to 23% when most myoblasts had fused into myotubes. Optimization of transfection conditions with three different vectors yielded efficiencies exceeding 50%. Thus, lipid-based transfection into primary skeletal muscle cells can be several times more efficient than previously reported., (Copyright 2002 Wiley Periodicals, Inc.)
- Published
- 2002
- Full Text
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36. Synapse-forming axons and recombinant agrin induce microprocess formation on myotubes.
- Author
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Uhm CS, Neuhuber B, Lowe B, Crocker V, and Daniels MP
- Subjects
- Agrin biosynthesis, Agrin genetics, Animals, Axons ultrastructure, Cell Surface Extensions drug effects, Cell Surface Extensions ultrastructure, Cells, Cultured, Coculture Techniques, Dendrites physiology, Dendrites ultrastructure, Epidermal Growth Factor pharmacology, Green Fluorescent Proteins, Growth Cones physiology, Growth Cones ultrastructure, Luminescent Proteins genetics, Microscopy, Electron, Microscopy, Fluorescence, Muscle, Skeletal embryology, Muscle, Skeletal metabolism, Muscle, Skeletal ultrastructure, Neuromuscular Junction drug effects, Neuromuscular Junction metabolism, Neuromuscular Junction ultrastructure, Neurons metabolism, Neurons ultrastructure, Pseudopodia drug effects, Pseudopodia ultrastructure, Rats, Receptor Aggregation physiology, Receptors, Cholinergic metabolism, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins pharmacology, Signal Transduction, Synapses ultrastructure, Transfection, Agrin pharmacology, Axons physiology, Muscle, Skeletal drug effects, Neurons drug effects, Synapses physiology
- Abstract
We examined cell-surface behavior at nerve-muscle contacts during synaptogenesis in cocultures of rat ventral spinal cord (VSC) neurons and myotubes. Developing synapses in 1-d-old cocultures were identified by the presence of axon-induced acetylcholine receptor (AChR) aggregation. Identified regions were then examined by transmission and scanning electron microscopy. The myotube surface near contacts with axons that induced AChR aggregation typically displayed ruffles, microvilli, and filopodia (microprocesses), indicating motility of the myotube surface. At some of these contact sites microprocesses were wrapped around the axon, resulting in the partial or total "submersion" of the axon within the myotube contours. Sites of myotube contact with somata and dendrites of the same neurons showed much less evidence of motility and surface interaction than sites of contact with axons. Moreover, the distance between opposed membranes of axons and myotubes was smaller than between dendrites or somata and myotubes, suggesting stronger adhesion of axons. These results suggest polarized expression of molecules involved in the induction of microprocess formation and adhesion in developing VSC neurons. We therefore tested the ability of agrin, which is preferentially secreted by axons, to induce microprocess formation in myotubes. Addition of recombinant C-terminal agrin to culture medium resulted in formation of microprocesses within 3 hr. Myotubes transfected with full-length rat agrin constructs displayed numerous filopodia, as revealed by fluorescence microscopy. The results suggest that the induction of muscle cell surface motility may be linked to the signaling processes that trigger the initial formation of the neuromuscular junction.
- Published
- 2001
37. Regulation of myosin heavy chain expression during rat skeletal muscle development in vitro.
- Author
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Torgan CE and Daniels MP
- Subjects
- Animals, Calcineurin genetics, Cells, Cultured, Cyclosporine pharmacology, DNA-Binding Proteins metabolism, Enzyme Inhibitors pharmacology, Immunoblotting, Microscopy, Fluorescence, Muscle Fibers, Skeletal cytology, Muscle Fibers, Skeletal drug effects, Muscle, Skeletal cytology, Muscle, Skeletal metabolism, Myosin Heavy Chains genetics, NFATC Transcription Factors, Protein Isoforms genetics, Protein Isoforms metabolism, Rats, Signal Transduction physiology, Tetrodotoxin pharmacology, Transcription Factors metabolism, Transfection, Calcineurin metabolism, Muscle Contraction physiology, Muscle Development, Muscle Fibers, Skeletal physiology, Muscle, Skeletal growth & development, Myosin Heavy Chains metabolism, Nuclear Proteins
- Abstract
Signals that determine fast- and slow-twitch phenotypes of skeletal muscle fibers are thought to stem from depolarization, with concomitant contraction and activation of calcium-dependent pathways. We examined the roles of contraction and activation of calcineurin (CN) in regulation of slow and fast myosin heavy chain (MHC) protein expression during muscle fiber formation in vitro. Myotubes formed from embryonic day 21 rat myoblasts contracted spontaneously, and approximately 10% expressed slow MHC after 12 d in culture, as seen by immunofluorescent staining. Transfection with a constitutively active form of calcineurin (CN*) increased slow MHC by 2.5-fold as determined by Western blot. This effect was attenuated 35% by treatment with tetrodotoxin and 90% by administration of the selective inhibitor of CN, cyclosporin A. Conversely, cyclosporin A alone increased fast MHC by twofold. Cotransfection with VIVIT, a peptide that selectively inhibits calcineurin-induced activation of the nuclear factor of activated T-cells, blocked the effect of CN* on slow MHC by 70% but had no effect on fast MHC. The results suggest that contractile activity-dependent expression of slow MHC is mediated largely through the CN-nuclear factor of activated T-cells pathway, whereas suppression of fast MHC expression may be independent of nuclear factor of activated T-cells.
- Published
- 2001
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- View/download PDF
38. Integrins stimulate phosphorylation of neurofilament NF-M subunit KSP repeats through activation of extracellular regulated-kinases (Erk1/Erk2) in cultured motoneurons and transfected NIH 3T3 cells.
- Author
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Li BS, Daniels MP, and Pant HC
- Subjects
- 3T3 Cells, Animals, Cells, Cultured, Enzyme Activation physiology, Fibronectins pharmacology, Laminin pharmacology, Mice, Neurofilament Proteins chemistry, Phosphorylation drug effects, Protein Isoforms metabolism, Rats, Transfection, Integrins physiology, Mitogen-Activated Protein Kinases metabolism, Motor Neurons metabolism, Neurofilament Proteins metabolism
- Abstract
Integrin-mediated interactions of cells with components of the extracellular matrix (ECM) regulate cell survival, cell proliferation, cell differentiation and cell migration through activation of multiple intracellular signal transduction pathways. In this study, we have demonstrated that integrin-matrix interactions promote KSP tail-domain phosphorylation of neurofilament medium molecular weight subunits (NF-M) in cultured rat spinal cord motoneurons and NF-M transfected NIH 3T3 cells. We found that laminin and fibronectin induce NF-M tail-domain phosphorylation in motoneurons and NIH 3T3 cells transfected with NF-M, respectively. This phosphorylation was selectively inhibited by PD98059, a specific MEK1 inhibitor. This suggests that laminin and fibronectin-induced MEK1 activation and the downstream targets Erk1 and Erk2 are involved in NF-M KSP tail-domain phosphorylation. This pathway appears to represent one of the mechanisms whereby integrin-extracellular matrix interactions are involved in phosphorylation of the NF-M KSP tail domain.
- Published
- 2001
- Full Text
- View/download PDF
39. Synaptic localization and axonal targeting of agrin secreted by ventral spinal cord neurons in culture.
- Author
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Ma J, Lugo B, Shah S, Godfrey EW, and Daniels MP
- Subjects
- Animals, Cells, Cultured, Chick Embryo, Coculture Techniques, Motor Neurons metabolism, Muscles cytology, Muscles embryology, Muscles metabolism, Rats, Spinal Cord cytology, Spinal Cord metabolism, Tissue Distribution, Agrin metabolism, Axons metabolism, Neurons metabolism, Spinal Cord embryology, Synapses metabolism
- Abstract
Agrin secreted by motor neurons is a critical signal for postsynaptic differentiation at the developing neuromuscular junction. We used cultures of chick ventral spinal cord neurons with rat myotubes and immunofluorescence with species-specific antibodies to determine the distribution of agrin secreted by neurons and compare it to the distribution of agrin secreted by myotubes. In addition, we determined the distribution of agrin secreted by isolated chick ventral spinal cord neurons and rat motor neurons grown on a substrate that binds agrin. In cocultures, neuronal agrin was concentrated along axons at sites of axon-induced acetylcholine receptor (AChR) aggregation and was found at every such synaptic site, consistent with its role in synaptogenesis. Smaller amounts of agrin were found on dendrites and cell bodies and rarely were associated with AChR aggregation. Muscle agrin, recognized by an antibody against rat agrin, was found at nonsynaptic sites of AChR aggregation but was not detected at synaptic sites, in contrast to neuronal agrin. In cultures of isolated chick neurons or rat motor neurons, agrin was deposited relatively uniformly around axons and dendrites during the first 2-3 days in culture. In older cultures, agrin immunoreactivity was markedly more intense around axons than dendrites, indicating that motor neurons possess an intrinsic, developmentally regulated program to target agrin secretion to axons., (Copyright 2000 John Wiley & Sons, Inc.)
- Published
- 2000
- Full Text
- View/download PDF
40. Rodent nerve-muscle cell culture system for studies of neuromuscular junction development: refinements and applications.
- Author
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Daniels MP, Lowe BT, Shah S, Ma J, Samuelsson SJ, Lugo B, Parakh T, and Uhm CS
- Subjects
- Agrin genetics, Agrin metabolism, Alternative Splicing, Animals, Axons metabolism, Cell Culture Techniques methods, Cells, Cultured, Coculture Techniques, Gene Expression, Motor Neurons metabolism, Neuromuscular Junction metabolism, Neurons metabolism, Protein Isoforms genetics, Protein Isoforms metabolism, Rats, Receptor Aggregation, Receptors, Cholinergic metabolism, Muscle, Skeletal cytology, Neuromuscular Junction growth & development, Neurons cytology
- Abstract
Understanding of vertebrate neuromuscular junction (NMJ) development has been advanced by experimentation with cultures of dissociated embryonic nerve and skeletal muscle cells, particularly those derived from Xenopus and chick embryos. We previously developed a rodent (rat) nerve-muscle coculture system that is characterized by extensive induction of acetylcholine receptor (AChR) aggregation at sites of axonal contact with myotubes (Dutton et al., 1995). In this article, we report modifications of this culture system and examples of its application to the study of NMJ development: (1) We describe improved methods for the enrichment of myoblasts to give higher yields of myotubes with equal or greater purity. (2) We demonstrate lipophilic dye labeling of axons in cocultures by injection of dye into neuron aggregates and show the feasibility of studying the growth of living axons on myotubes during synapse formation. (3) We describe the preparation of a better-defined coculture system containing myotubes with purified rat motoneurons and characterize the system with respect to axon-induced AChR aggregation. (4) We demonstrate dependence of the pattern of axon-induced AChR aggregation on muscle cell species, by the use of chick-rat chimeric co-cultures. (5) We provide evidence for the role of alternatively-spliced agrin isoforms in synapse formation by using single cell RT-PCR with neurons collected from co-cultures after observation of axon-induced AChR aggregation. Microsc. Res. Tech. 49:26-37, 2000. Published 2000 Wiley-Liss, Inc.
- Published
- 2000
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41. ERG30, a VAP-33-related protein, functions in protein transport mediated by COPI vesicles.
- Author
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Soussan L, Burakov D, Daniels MP, Toister-Achituv M, Porat A, Yarden Y, and Elazar Z
- Subjects
- Amino Acid Sequence, Animals, Antibodies pharmacology, Base Sequence, Biological Transport drug effects, Carrier Proteins chemistry, Carrier Proteins immunology, Cell Line, Cloning, Molecular, Coated Vesicles drug effects, Coatomer Protein, Endoplasmic Reticulum, Rough metabolism, Gene Expression, Golgi Apparatus drug effects, Golgi Apparatus metabolism, Membrane Proteins chemistry, Membrane Proteins immunology, Molecular Sequence Data, Protein Binding, Protein Structure, Secondary, Rats, SNARE Proteins, Sequence Deletion, Sequence Homology, Amino Acid, Yeasts genetics, Carrier Proteins genetics, Carrier Proteins metabolism, Coated Vesicles metabolism, Membrane Proteins genetics, Membrane Proteins metabolism, Membrane Proteins physiology, Vesicular Transport Proteins
- Abstract
Intracellular transport of newly synthesized and mature proteins via vesicles is controlled by a large group of proteins. Here we describe a ubiquitous rat protein-endoplasmic reticulum (ER) and Golgi 30-kD protein (ERG30)-which shares structural characteristics with VAP-33, a 33-kD protein from Aplysia californica which was shown to interact with the synaptic protein VAMP. The transmembrane topology of the 30-kD ERG30 corresponds to a type II integral membrane protein, whose cytoplasmic NH(2) terminus contains a predicted coiled-coil motif. We localized ERG30 to the ER and to pre-Golgi intermediates by biochemical and immunocytochemical methods. Consistent with a role in vesicular transport, anti-ERG30 antibodies specifically inhibit intra-Golgi transport in vitro, leading to significant accumulation of COPI-coated vesicles. It appears that ERG30 functions early in the secretory pathway, probably within the Golgi and between the Golgi and the ER.
- Published
- 1999
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42. Altered expression of tropomodulin in cardiomyocytes disrupts the sarcomeric structure of myofibrils.
- Author
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Sussman MA, Baqué S, Uhm CS, Daniels MP, Price RL, Simpson D, Terracio L, and Kedes L
- Subjects
- Actins metabolism, Adenoviridae genetics, Adenoviridae physiology, Animals, Carrier Proteins physiology, Cells, Cultured, DNA, Recombinant, Gene Expression genetics, Gene Expression physiology, Genetic Vectors genetics, Humans, Mice, Myocardium cytology, Myofibrils ultrastructure, Myofibrils virology, Proteins metabolism, RNA, Messenger metabolism, RNA, Viral metabolism, Rats, Sarcomeres chemistry, Sarcomeres ultrastructure, Sarcomeres virology, Transfection genetics, Transfection physiology, Tropomodulin, Carrier Proteins genetics, Microfilament Proteins, Myocardium metabolism, Myofibrils metabolism
- Abstract
Tropomodulin is a tropomyosin-binding protein that terminates "pointed-end" actin filament polymerization. To test the hypothesis that regulation of tropomodulin:actin filament stoichiometry is critical for maintenance of actin filament length, tropomodulin levels were altered in cells by infection with recombinant adenoviral expression vectors, which produce either sense or antisense tropomodulin mRNA. Neonatal rat cardiomyocytes were infected, and sarcomeric actin filament organization was examined. Confocal microscopy indicated that overexpression of tropomodulin protein shortened actin filaments and caused myofibril degeneration. In contrast, decreased tropomodulin content resulted in the formation of abnormally long actin filament bundles. Despite changes in myofibril structure caused by altered tropomodulin expression, total protein turnover of the cardiomyocytes was unaffected. Biochemical analyses of infected cardiomyocytes indicated that changes in actin distribution, rather than altered actin content, accounted for myofibril reorganization. Ultrastructural analysis showed thin-filament disarray and revealed the presence of leptomeres after tropomodulin overexpression. Tropomodulin-mediated effects constitute a novel mechanism to control actin filaments, and our findings demonstrate that regulated tropomodulin expression is necessary to maintain stabilized actin filament structures in cardiac muscle cells.
- Published
- 1998
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43. Intercellular communication that mediates formation of the neuromuscular junction.
- Author
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Daniels MP
- Subjects
- Animals, Ascorbic Acid metabolism, Calcitonin Gene-Related Peptide physiology, Genetic Techniques, Humans, Models, Biological, Muscle Fibers, Skeletal physiology, Neurons physiology, Receptors, Cholinergic biosynthesis, Signal Transduction, Cell Communication physiology, Neuromuscular Junction physiology, Receptors, Cholinergic physiology, Synapses physiology
- Abstract
Reciprocal signals between the motor axon and myofiber induce structural and functional differentiation in the developing neuromuscular junction (NMJ). Elevation of presynaptic acetylcholine (ACh) release on nerve-muscle contact and the correlated increase in axonal-free calcium are triggered by unidentified membrane molecules. Restriction of axon growth to the developing NMJ and formation of active zones for ACh release in the presynaptic terminal may be induced by molecules in the synaptic basal lamina, such as S-laminin, heparin binding growth factors, and agrin. Acetylcholine receptor (AChR) synthesis by muscle cells may be increased by calcitonin gene-related peptide (CGRP), ascorbic acid, and AChR-inducing activity (ARIA)/heregulin, which is the best-established regulator. Heparin binding growth factors, proteases, adhesion molecules, and agrin all may be involved in the induction of AChR redistribution to form postsynaptic-like aggregates. However, the strongest case has been made for agrin's involvement. "Knockout" experiments have implicated agrin as a primary anterograde signal for postsynaptic differentiation and muscle-specific kinase (MuSK), as a putative agrin receptor. It is likely that both presynaptic and postsynaptic differentiation are induced by multiple molecular signals. Future research should reveal the physiological roles of different molecules, their interactions, and the identity of other molecular participants.
- Published
- 1997
- Full Text
- View/download PDF
44. Chicken skeletal muscle tropomodulin: novel localization and characterization.
- Author
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Sussman MA, Ito M, Daniels MP, Flucher B, Buranen S, and Kedes L
- Subjects
- Actins metabolism, Amino Acids analysis, Animals, Carrier Proteins analysis, Cloning, Molecular, DNA biosynthesis, Erythrocytes metabolism, Fluorescent Antibody Technique, Direct, Immunoblotting, Muscle, Skeletal ultrastructure, Myofibrils metabolism, RNA, Messenger biosynthesis, Sarcomeres metabolism, Sarcomeres ultrastructure, Tropomodulin, Carrier Proteins metabolism, Chickens metabolism, Microfilament Proteins, Muscle, Skeletal metabolism
- Abstract
Tropomodulin is a 40.6-kDa isoform-specific tropomyosin-binding protein which inhibits actin filament elongation from the slow-growing (pointed) end and localizes at or near the pointed ends of thin filaments in rat skeletal muscle. Immunofluorescent localization using affinity-purified anti-tropomodulin antibodies in avian myofibril preparations demonstrates novel immunoreactivity at the Z-disc in addition to the previously reported localization at the periphery of I-Z-I brushes where actin filaments terminate. Identical results were obtained using antibody preparations generated against either bacterially expressed tropomodulin or human erythrocyte tropomodulin. Chicken muscle preparations contain Mr 43000 polypeptides which bind antibodies generated against tropomodulin in Western blot analysis, as well as 125I-labeled tropomyosin in blot overlays. Tropomodulin mRNA expression in adult muscle was confirmed by RNase protection assays, and the sequence of our tropomodulin cDNA amplified from chicken muscle mRNA preparations by polymerase chain reaction closely matches clones selected by chicken muscle cDNA library screening. The novel immunolocalization we report raises new possibilities for the role of tropomodulin in the organization of avian skeletal muscle at the Z-disc. We conclude that tropomodulin is likely to be important in striated muscle biology as a structural component in the Z-disc region which participates in the process of thin filament organization and assembly.
- Published
- 1996
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45. Molecular organization of transverse tubule/sarcoplasmic reticulum junctions during development of excitation-contraction coupling in skeletal muscle.
- Author
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Flucher BE, Andrews SB, and Daniels MP
- Subjects
- Action Potentials, Animals, Calcium metabolism, Calcium Channels metabolism, Calcium Channels, L-Type, Cells, Cultured, Fluorescent Antibody Technique, Microscopy, Electron, Muscle Contraction physiology, Muscle Proteins metabolism, Muscle, Skeletal physiology, Muscle, Skeletal ultrastructure, Rats, Ryanodine Receptor Calcium Release Channel, Sarcoplasmic Reticulum physiology, Sarcoplasmic Reticulum ultrastructure, Tissue Distribution, Muscle, Skeletal embryology
- Abstract
The relationship between the molecular composition and organization of the triad junction and the development of excitation-contraction (E-C) coupling was investigated in cultured skeletal muscle. Action potential-induced calcium transients develop concomitantly with the first expression of the dihydropyridine receptor (DHPR) and the ryanodine receptor (RyR), which are colocalized in clusters from the time of their earliest appearance. These DHPR/RyR clusters correspond to junctional domains of the transverse tubules (T-tubules) and sarcoplasmic reticulum (SR), respectively. Thus, at first contact T-tubules and SR form molecularly and structurally specialized membrane domains that support E-C coupling. The earliest T-tubule/SR junctions show structural characteristics of mature triads but are diverse in conformation and typically are formed before the extensive development of myofibrils. Whereas the initial formation of T-tubule/SR junctions is independent of association with myofibrils, the reorganization into proper triads occurs as junctions become associated with the border between the A band and the I band of the sarcomere. This final step in triad formation manifests itself in an increased density and uniformity of junctions in the cytoplasm, which in turn results in increased calcium release and reuptake rates.
- Published
- 1994
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46. Tropomodulin is associated with the free (pointed) ends of the thin filaments in rat skeletal muscle.
- Author
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Fowler VM, Sussmann MA, Miller PG, Flucher BE, and Daniels MP
- Subjects
- Animals, Antibodies, Electrophoresis, Polyacrylamide Gel, Fluorescent Antibody Technique, Molecular Weight, Muscles chemistry, Rats, Tropomodulin, Actin Cytoskeleton ultrastructure, Carrier Proteins analysis, Microfilament Proteins, Muscle Proteins analysis, Muscles ultrastructure, Myofibrils ultrastructure, Sarcomeres ultrastructure
- Abstract
The length and spatial organization of thin filaments in skeletal muscle sarcomeres are precisely maintained and are essential for efficient muscle contraction. While the major structural components of skeletal muscle sarcomeres have been well characterized, the mechanisms that regulate thin filament length and spatial organization are not well understood. Tropomodulin is a new, 40.6-kD tropomyosin-binding protein from the human erythrocyte membrane skeleton that binds to one end of erythrocyte tropomyosin and blocks head-to-tail association of tropomyosin molecules along actin filaments. Here we show that rat psoas skeletal muscle contains tropomodulin based on immunoreactivity, identical apparent mobility on SDS gels, and ability to bind muscle tropomyosin. Results from immunofluorescence labeling of isolated myofibrils at resting and stretched lengths using anti-erythrocyte tropomodulin antibodies indicate that tropomodulin is localized at or near the free (pointed) ends of the thin filaments; this localization is not dependent on the presence of myosin thick filaments. Immunoblotting of supernatants and pellets obtained after extraction of myosin from myofibrils also indicates that tropomodulin remains associated with the thin filaments. 1.2-1.6 copies of muscle tropomodulin are present per thin filament in myofibrils, supporting the possibility that one or two tropomodulin molecules may be associated with the two terminal tropomyosin molecules at the pointed end of each thin filament. Although a number of proteins are associated with the barbed ends of the thin filaments at the Z disc, tropomodulin is the first protein to be specifically located at or near the pointed ends of the thin filaments. We propose that tropomodulin may cap the tropomyosin polymers at the pointed end of the thin filament and play a role in regulating thin filament length.
- Published
- 1993
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47. Coordinated development of myofibrils, sarcoplasmic reticulum and transverse tubules in normal and dysgenic mouse skeletal muscle, in vivo and in vitro.
- Author
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Flucher BE, Phillips JL, Powell JA, Andrews SB, and Daniels MP
- Subjects
- Animals, Animals, Newborn, Cells, Cultured, Crosses, Genetic, Embryo, Mammalian, Fluorescent Antibody Technique, Gestational Age, Homozygote, Mice, Mice, Mutant Strains, Muscles embryology, Muscles pathology, Muscle Proteins analysis, Muscles ultrastructure, Myofibrils ultrastructure, Sarcomeres ultrastructure, Sarcoplasmic Reticulum ultrastructure
- Abstract
We studied the development of transverse (T)-tubules and sarcoplasmic reticulum (SR) in relationship to myofibrillogenesis in normal and dysgenic (mdg/mdg) mouse skeletal muscle by immunofluorescent labeling of specific membrane and myofibrillar proteins. At E16 the development of the myofibrils and membranes in dysgenic and normal diaphragm was indistinguishable, including well developed myofibrils, a delicate network of T-tubules, and a prominent SR which was not yet cross-striated. In diaphragms of E18 dysgenic mice, both the number and size of muscle fibers and myofibrillar organization were deficient in comparison to normal diaphragms, as previously reported. T-tubule labeling was abnormal, showing only scattered tubules and fragments. However, many muscle fibers displayed cross striation of sarcomeric proteins and SR comparable to normal muscle. In cultured myotubes, cross-striated organization of sarcomeric proteins proceeded essentially in two stages: first around the Z-line and later in the A-band. Sarcomeric organization of the SR coincided with the first stage, while the appearance of T-tubules in the mature transverse orientation occurred infrequently, only after A-band maturation. In culture, myofibrillar and membrane organization was equivalent in normal and dysgenic muscle at the earlier stage of development, but half as many dysgenic myotubes reached the later stage as compared to normal. We conclude that the mdg mutation has little effect on the initial stage of membrane and myofibril development and that the deficiencies often seen at later stages result indirectly from the previously described absence of dihydropyridine receptor function in the mutant.
- Published
- 1992
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48. Biogenesis of transverse tubules in skeletal muscle in vitro.
- Author
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Flucher BE, Terasaki M, Chin HM, Beeler TJ, and Daniels MP
- Subjects
- Animals, Carbocyanines, Cell Membrane ultrastructure, Cells, Cultured, Embryo, Nonmammalian, Fluorescent Antibody Technique, Fluorescent Dyes, Immunoblotting, Mice, Microscopy, Immunoelectron, Muscles physiology, Xenopus laevis, Microtubules ultrastructure, Muscles ultrastructure
- Abstract
The transverse (T) tubules of skeletal muscle are membrane tubules that are continuous with the plasma membrane and penetrate the mature muscle fiber radially to carry surface membrane depolarization to the sites of excitation-contraction coupling. We have studied the development of the T-tubule system in cultured amphibian and mammalian muscle cells using a fluorescent lipid probe and antibodies against T-tubules and plasma membranes. Both the lipid probe and the T-tubule antibody recognized an extensive tubular membrane system which subsequently differentiated into the T-system. At all developmental stages, the molecular composition of the T-system was distinct from that of the plasma membrane, suggesting that during myogenesis T-tubules and the plasma membrane form independently from each other and that exchange of membrane proteins between the two continuous compartments is restricted. In rat muscle cultures, T-tubule-specific antigens were first expressed in terminally differentiated myoblasts. Prior to myoblast fusion the antigens appeared as punctate label throughout the cytoplasm. Shortly after fusion the T-tubule-specific antibody labeled a tubular membrane system that extended from the perinuclear region and penetrated most parts of the cells. In contrast, the lipid probe, which labels the T-tubules by virtue of their direct continuity with the plasma membrane, only labeled short tubules extending from the plasma membrane into the periphery of the myotubes at the early stage in development. Thus, the assembly of the T-tubules appears to begin before their connections with the plasma membrane are established.
- Published
- 1991
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- View/download PDF
49. Localization of actin, beta-spectrin, 43 x 10(3) Mr and 58 x 10(3) Mr proteins to receptor-enriched domains of newly formed acetylcholine receptor aggregates in isolated myotube membranes.
- Author
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Daniels MP
- Subjects
- Animals, Cell Membrane metabolism, Cells, Cultured, Clathrin metabolism, Cytoskeletal Proteins metabolism, Cytoskeleton metabolism, Fluorescent Antibody Technique, Muscles ultrastructure, Proteins metabolism, Rats, Talin, Vinculin, Actins metabolism, Muscles metabolism, Receptor Aggregation, Receptors, Cholinergic metabolism, Spectrin metabolism
- Abstract
I have examined the possible involvement of specific cytoskeletal and peripheral membrane proteins in the early stages of acetylcholine receptor (AChR) aggregation in rat myotubes in culture by immunofluorescence localization of these proteins on the cytoplasmic face of isolated plasma membranes. A culture procedure utilizing selective replating of myoblasts and subsequent treatment with cytosine arabinoside was devised to obtain large, multipolar myotubes with extensive upper surfaces that are free of fibroblasts. These cultures were exposed for 4-6 h to embryonic pig brain extract (EBX) to induce AChR aggregate formation on the upper cell surface, and the AChRs were labeled with TRITC-conjugated alpha-bungarotoxin. Large sheets of plasma membranes from the upper cell surface were isolated by adhesion to a coverslip coated with a polypeptide adhesive (Cell-Tak) that was pressed on top of the culture. The membranes were labeled by indirect immunofluorescence with monoclonal antibodies against the 43 x 10(3) Mr and 58 x 10(3) Mr proteins, originally identified in the AChR-enriched membranes of Torpedo electroplaques, and with monoclonal antibodies against isoforms of actin and beta-spectrin. The labeling patterns showed that all four of these proteins are concentrated in the punctate AChR-enriched domains within the aggregates, suggesting that they may be involved in the early stages of AChR aggregation. Immunofluorescence labeling with monoclonal antibodies against vinculin and clathrin, and with an antiserum to talin, showed that these proteins are also associated with AChR aggregates; however, their labeling patterns did not correspond closely to the AChR-enriched domains. Furthermore, vinculin and talin dissociated from most of the membrane during isolation. The concentration of beta-spectrin and actin isoforms on the cytoplasmic fact of the AChR-enriched domains is consistent with the formation, early in the aggregation process, of a membrane-cytoskeleton association similar to that of erythrocytes.
- Published
- 1990
- Full Text
- View/download PDF
50. Localization of the alpha 1 and alpha 2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triads.
- Author
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Flucher BE, Morton ME, Froehner SC, and Daniels MP
- Subjects
- Animals, Ankyrins, Calcium Channels, Fluorescent Antibody Technique, Gold, Immunologic Techniques, Microscopy, Electron, Muscles ultrastructure, Rats, Tissue Distribution, Blood Proteins analysis, Membrane Proteins analysis, Muscles analysis, Receptors, Nicotinic analysis
- Abstract
We have studied the subcellular distribution of the alpha 1 and alpha 2 subunits of the dihydropyridine (DHP) receptor and ankyrin in rat skeletal muscle with immunofluorescence and immunogold labeling techniques. All three proteins were concentrated in the triad junction formed between the T-tubules and sarcoplasmic reticulum. The alpha 1 and alpha 2 subunits of the DHP receptor were colocalized in the junctional T-tubule membrane, supporting their proposed association in a functional complex and the possible participation of the alpha 2 subunit in excitation-contraction coupling. Ankyrin label in the triad showed a distribution different from that of the DHP receptor subunits. In addition, ankyrin was found in longitudinally oriented structures outside the triad. Thus, ankyrin might be involved in organizing the triad and in immobilizing integral membrane proteins in T-tubules and the sarcoplasmic reticulum.
- Published
- 1990
- Full Text
- View/download PDF
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