10 results on '"Daniele Dipresa"'
Search Results
2. Hypothermic preservation of endothelialized gas-exchange membranes
- Author
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Axel Haverich, Adim De, Ariana Peredo, Daniele Dipresa, Hayan Merhej, Willem F. Wolkers, Bettina Wiegmann, Michael Pflaum, and Sotirios Korossis
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Oxygenators ,0206 medical engineering ,Biomedical Engineering ,Cell Culture Techniques ,Medicine (miscellaneous) ,Bioengineering ,02 engineering and technology ,030204 cardiovascular system & hematology ,Biomaterials ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Massive disruption ,Extracorporeal Membrane Oxygenation ,Refrigeration ,Monolayer ,Human Umbilical Vein Endothelial Cells ,Humans ,Cells, Cultured ,Chemistry ,Stem Cells ,Polymethylpentene ,Membranes, Artificial ,Thrombosis ,General Medicine ,020601 biomedical engineering ,Cold Temperature ,Membrane ,Normal growth ,Feasibility Studies ,Biomedical engineering - Abstract
Endothelialization of the blood contacting surfaces of blood-contacting medical devices, such as cardiovascular prostheses or biohybrid oxygenators, represents a plausible strategy for increasing their hemocompatibility. Nevertheless, isolation and expansion of autologous endothelial cells (ECs) usually requires multiple processing steps and time to obtain sufficient cell numbers. This excludes endothelialization from application in acute situations. Off-the-shelf availability of cell-seeded biohybrid devices could be potentially facilitated by hypothermic storage. In this study, the survival of cord-blood-derived endothelial colony forming cells (ECFCs) that were seeded onto polymethylpentene (PMP) gas-exchange membranes and stored for up to 2 weeks in different commercially available and commonly used preservation media was measured. While storage at 4°C in normal growth medium (EGM-2) for 3 days resulted in massive disruption of the ECFC monolayer and a significant decline in viability, ECFC monolayers preserved in Chillprotec could recover after up to 14 days with negligible effects on their integrity and viability. ECFC monolayers preserved in Celsior, HTS-FRS, or Rokepie medium showed a significant decrease in viability after 7 days or longer periods. These results demonstrated the feasibility of hypothermic preservation of ECFC monolayers on gas-exchange membranes for up to 2 weeks, with potential application on the preservation of pre-endothelialized oxygenators and further biohybrid cardiovascular devices.
- Published
- 2020
3. Hemodynamic Assessment of Hollow-Fiber Membrane Oxygenators Using Computational Fluid Dynamics in Heterogeneous Membrane Models
- Author
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Bettina Wiegmann, Panagiotis Kalozoumis, Daniele Dipresa, Ariana Peredo, Michael Pflaum, Sotirios Korossis, and Axel Haverich
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Oxygenators ,Materials science ,Biomedical Engineering ,Thrombogenicity ,Blood flow ,Artificial lung ,Hollow fiber membrane ,Physiology (medical) ,Blood oxygenator ,Shear stress ,Hydrodynamics ,Oxygenator ,Biomedical engineering ,Oxygenators, Membrane - Abstract
Extracorporeal membrane oxygenation (ECMO) has been used clinically for more than 40 years as a bridge to transplantation, with hollow-fiber membrane (HFM) oxygenators gaining in popularity due to their high gas transfer and low flow resistance. In spite of the technological advances in ECMO devices, the inevitable contact of the perfused blood with the polymer hollow-fiber gas-exchange membrane, and the subsequent thrombus formation, limits their clinical usage to only 2–4 weeks. In addition, the inhomogeneous flow in the device can further enhance thrombus formation and limit gas-transport efficiency. Endothelialization of the blood contacting surfaces of ECMO devices offers a potential solution to their inherent thrombogenicity. However, abnormal shear stresses and inhomogeneous blood flow might affect the function and activation status of the seeded endothelial cells (ECs). In this study, the blood flow through two HFM oxygenators, including the commercially available iLA® MiniLung Petite Novalung (Xenios AG, Germany) and an experimental one for the rat animal model, was modeled using computational fluid dynamics (CFD), with a view to assessing the magnitude and distribution of the wall shear stress (WSS) on the hollow fibers and flow fields in the oxygenators. This work demonstrated significant inhomogeneity in the flow dynamics of both oxygenators, with regions of high hollow-fiber WSS and regions of stagnant flow, implying a variable flow-induced stimulation on seeded ECs and possible EC activation and damage in a biohybrid oxygenator setting.
- Published
- 2020
4. Vitrified Human Umbilical Arteries as Potential Grafts for Vascular Tissue Engineering
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Sotiris Korossis, Efstathios Michalopoulos, Aggeliki Papapanagiotou, Michalis Katsimpoulas, Eva Kassi, Daniele Dipresa, Catherine Stavropoulos-Giokas, Panagiotis Mallis, and Alkiviadis Kostakis
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Carotid Artery, Common ,Swine ,0206 medical engineering ,Biomedical Engineering ,Medicine (miscellaneous) ,02 engineering and technology ,Cryopreservation ,Umbilical Arteries ,Glycosaminoglycan ,Extracellular matrix ,03 medical and health sciences ,Hydroxyproline ,chemistry.chemical_compound ,medicine.artery ,medicine ,Animals ,Humans ,Vitrification ,Common carotid artery ,Sodium dodecyl sulfate ,030304 developmental biology ,0303 health sciences ,Extracellular Matrix Proteins ,Decellularization ,Tissue Engineering ,Tissue Scaffolds ,Sodium Dodecyl Sulfate ,Arteries ,020601 biomedical engineering ,Blood Vessel Prosthesis ,Extracellular Matrix ,Carotid Arteries ,chemistry ,Original Article ,Biomedical engineering - Abstract
BACKGROUND: The development of a biological based small diameter vascular graft (d
- Published
- 2019
5. Endothelialization and characterization of titanium dioxide-coated gas-exchange membranes for application in the bioartificial lung
- Author
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Sabrina Schmeckebier, Bettina Wiegmann, Axel Haverich, Kanchan Chauhan, Michael Pflaum, Sotirios Korossis, Marina Kühn-Kauffeldt, Rolf J. Haug, Daniele Dipresa, and Jochen Schein
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Blood Platelets ,Materials science ,Surface Properties ,0206 medical engineering ,Biomedical Engineering ,HL-60 Cells ,Nanotechnology ,Polyenes ,02 engineering and technology ,engineering.material ,Real-Time Polymerase Chain Reaction ,Biochemistry ,Dip-coating ,Biomaterials ,chemistry.chemical_compound ,Oxygen permeability ,Platelet Adhesiveness ,Coated Materials, Biocompatible ,Coating ,Monolayer ,Cell Adhesion ,Human Umbilical Vein Endothelial Cells ,Humans ,Cell adhesion ,Lung ,Molecular Biology ,Cell Proliferation ,Titanium ,Bioartificial Organs ,Membranes, Artificial ,General Medicine ,Adhesion ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,Oxygen ,Membrane ,chemistry ,Titanium dioxide ,Biophysics ,engineering ,0210 nano-technology ,Biotechnology - Abstract
Fouling on the gas-exchange hollow-fiber membrane (HFM) of extracorporeal membrane oxygenation (ECMO) devices by blood components and pathogens represents the major hurdle to their long-term application in patients with lung deficiency or unstable hemodynamics. Although patients are treated with anticoagulants, deposition of blood proteins onto the membrane surface may still occur after few days, leading to insufficient gas transfer and, consequently, to device failure. The aim of this study was to establish an endothelial cell (EC) monolayer onto the gas-exchange membrane of an ECMO device with a view to developing a hemocompatible bioartificial lung. Poly(4-methyl-1-pentene) (PMP) gas-exchange membranes were coated with titanium dioxide (TiO2), using the pulsed vacuum cathodic arc plasma deposition (PVCAPD) technique, in order to generate a stable interlayer, enabling cell adhesion onto the strongly hydrophobic PMP membrane. The TiO2 coating reduced the oxygen transfer rate (OTR) of the membrane by 22%, and it successfully mediated EC attachment. The adhered ECs formed a confluent monolayer, which retained a non-thrombogenic state and showed cell-to-cell, as well as cell-to-substrate contacts. The established monolayer was able to withstand physiological shear stress and possessed a “self-healing” capacity at areas of induced monolayer disruption. The study demonstrated that the TiO2 coating mediated EC attachment and the establishment of a functional EC monolayer. Statement of Significance Surface endothelialization is considered an effective approach to achieve complete hamocompatibility of blood-contacting devices. Several strategies to enable endothelial cell adhesion onto stents and vascular prostheses have already been described in the literature. However, only few studies investigated the feasibility of establishing an endothelial monolayer onto the gas exchange membrane of ECMO devices, using peptides or proteins that were weakly adsorbed via dip coating techniques. This study demonstrated the effectiveness of an alternative and stable titanium dioxide coating for gas-exchange membranes, which enabled the establishment of a confluent, functional and non-activated endothelial monolayer, while maintaining oxygen permeability.
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- 2017
6. Effect of 'in air' freezing on post-thaw recovery of Callithrix jacchus mesenchymal stromal cells and properties of 3D collagen-hydroxyapatite scaffolds
- Author
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Sotirios Korossis, Bulat Sydykov, Willem F. Wolkers, Daniele Dipresa, Roland Scharf, Thaqif El Khassawna, Annemarie Beck, Dmitrii Ivnev, Oleksandr Gryshkov, Sven Knaack, Jan Belikan, Anja Lode, Alexander Y. Petrenko, Ramon Cabiscol, Vitalii Mutsenko, Michael Gelinsky, Marian Kampschulte, Lothar Lauterboeck, Birgit Glasmacher, and Dmytro Tarusin
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Sucrose ,Cell Survival ,Regenerative medicine ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,chemistry.chemical_compound ,Cryoprotective Agents ,On demand ,Freezing ,Animals ,Dimethyl Sulfoxide ,Health sector ,Biological Specimen Banks ,Slow freezing ,biology ,Tissue Engineering ,Dimethyl sulfoxide ,Mesenchymal stem cell ,Callithrix ,Mesenchymal Stem Cells ,General Medicine ,biology.organism_classification ,Durapatite ,chemistry ,Collagen ,General Agricultural and Biological Sciences ,Biomedical engineering - Abstract
Through enabling an efficient supply of cells and tissues in the health sector on demand, cryopreservation is increasingly becoming one of the mainstream technologies in rapid translation and commercialization of regenerative medicine research. Cryopreservation of tissue-engineered constructs (TECs) is an emerging trend that requires the development of practically competitive biobanking technologies. In our previous studies, we demonstrated that conventional slow-freezing using dimethyl sulfoxide (Me2SO) does not provide sufficient protection of mesenchymal stromal cells (MSCs) frozen in 3D collagen-hydroxyapatite scaffolds. After simple modifications to a cryopreservation protocol, we report on significantly improved cryopreservation of TECs. Porous 3D scaffolds were fabricated using freeze-drying of a mineralized collagen suspension and following chemical crosslinking. Amnion-derived MSCs from common marmoset monkey Callithrix jacchus were seeded onto scaffolds in static conditions. Cell-seeded scaffolds were subjected to 24 h pre-treatment with 100 mM sucrose and slow freezing in 10% Me2SO/20% FBS alone or supplemented with 300 mM sucrose. Scaffolds were frozen ‘in air’ and thawed using a two-step procedure. Diverse analytical methods were used for the interpretation of cryopreservation outcome for both cell-seeded and cell-free scaffolds. In both groups, cells exhibited their typical shape and well-preserved cell-cell and cell-matrix contacts after thawing. Moreover, viability test 24 h post-thaw demonstrated that application of sucrose in the cryoprotective solution preserves a significantly greater portion of sucrose-pretreated cells (more than 80%) in comparison to Me2SO alone (60%). No differences in overall protein structure and porosity of frozen scaffolds were revealed whereas their compressive stress was lower than in the control group. In conclusion, this approach holds promise for the cryopreservation of ‘ready-to-use’ TECs.
- Published
- 2019
7. Hydrogels for targeted waveguiding and light diffusion
- Author
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Maria Leilani Torres-Mapa, Daniele Dipresa, Sonja Johannsmeier, Dag Heinemann, Tammo Ripken, and Alexander Heisterkamp
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Materials science ,Polymer fiber ,Biocompatibility ,Poly(ethylene glycol) dimethacrylate ,Refractive index ,Dewey Decimal Classification::600 | Technik::620 | Ingenieurwissenschaften und Maschinenbau ,Nanotechnology ,02 engineering and technology ,01 natural sciences ,010309 optics ,chemistry.chemical_compound ,Incident light ,Light delivery ,0103 physical sciences ,Photomedicine ,Hydrogel composition ,Photon diffusion ,Easy fabrication ,chemistry.chemical_classification ,Polyethylene glycols ,technology, industry, and agriculture ,Hydrogels ,Polymer ,021001 nanoscience & nanotechnology ,Ray ,Electronic, Optical and Magnetic Materials ,chemistry ,Self-healing hydrogels ,Light diffusion ,Polystyrene ,Polystyrene particle ,ddc:620 ,0210 nano-technology ,Chemical stability ,Waveguides - Abstract
Advances in photomedicine and optogenetics have defined the problem of efficient light delivery in vivo. Recently, hydrogels have been proposed as alternatives to glass or polymer fibers. These materials provide remarkable versatility, biocompatibility and easy fabrication protocols. Here, we investigate the usability of waveguides from poly(ethylene glycol) dimethacrylate for targeted light delivery and diffusion. Different hydrogel compositions were characterized with regard to water content, chemical stability, elasticity, refractive index and optical losses. Differences in refractive index were introduced to achieve targeted light delivery, and scattering polystyrene particles were dispersed in the hydrogel samples to diffuse the incident light. Complex constructs were produced to demonstrate the versatility of hydrogel waveguides. © 2019 Optical Society of America.
- Published
- 2019
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8. Biocompatibility and Immunogenicity of Decellularized Allogeneic Aorta in the Orthotopic Rat Model
- Author
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Lucrezia Morticelli, Bisharah Soudah, Efstathios Michalopoulos, Axel Haverich, Panagiotis Mallis, Daniele Dipresa, Artemis Kouvaka, Ioanna Gontika, Michalis Katsimpoulas, Sotirios Korossis, and Ulrike Böer
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Male ,medicine.medical_specialty ,Biocompatibility ,Arterial disease ,0206 medical engineering ,Rat model ,Biomedical Engineering ,Bioengineering ,Biocompatible Materials ,02 engineering and technology ,Biochemistry ,Biomaterials ,03 medical and health sciences ,Antigens, CD ,medicine.artery ,medicine ,Animals ,Transplantation, Homologous ,Aorta ,030304 developmental biology ,0303 health sciences ,Decellularization ,business.industry ,Immunogenicity ,Abdominal aorta ,020601 biomedical engineering ,Immunohistochemistry ,Surgery ,Biomechanical Phenomena ,Rats ,medicine.anatomical_structure ,Models, Animal ,business ,Blood vessel - Abstract
The generation of a small-caliber arterial graft, utilizing a large vessel of a small animal, such as the aorta of the rat or rabbit, for clinical use in the peripheral arterial tree, can widen the options for arterial prostheses. This
- Published
- 2018
9. Developing a biohybrid lung – sufficient endothelialization of poly-4-methly-1-pentene gas exchange hollow-fiber membranes
- Author
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Michael Pflaum, Axel Haverich, Sabrina Schmeckebier, Heide von Seggern, Bettina Wiegmann, Sotirios Korossis, Klaus Höffler, Daniele Dipresa, and Jörg Seume
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Materials science ,Endothelium ,0206 medical engineering ,Cell Culture Techniques ,Biomedical Engineering ,02 engineering and technology ,Alkenes ,030204 cardiovascular system & hematology ,Biomaterials ,03 medical and health sciences ,0302 clinical medicine ,Monolayer ,medicine ,Fluorescence microscope ,Humans ,Lung ,Cells, Cultured ,Cell adhesion molecule ,Cell growth ,Albumin ,Endothelial Cells ,020601 biomedical engineering ,medicine.anatomical_structure ,Membrane ,Mechanics of Materials ,Cell culture ,Artificial Organs ,Biomedical engineering - Abstract
Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of human cord blood derived endothelial cells (HCBEC) on PMP-HFs were analyzed under static conditions by WST-8 cell proliferation assay and fluorescence microscopy. The HCBEC monolayer integrity under different flow conditions was also assessed. Endothelial-specific phenotype verification, expression activation levels and thrombogenic state markers were quantified by real-time RT-PCR for cell-to-PMP-HF contact under static and dynamic conditions. The results demonstrated the feasibility of establishing a viable, confluent, and flow-resistant endothelial monolayer on the blood-contact surface of PMP-HFs, which maintained a physiological response to TNFα-stimulation and flow conditions. The endothelial phenotype, expression levels of adhesion molecules and thrombogenic state markers were unaffected by cell-to-PMP-HFs contact. These results represent a significant step towards establishing a biohybrid lung.
- Published
- 2016
10. ‘In air’ cryopreservation of mesenchymal stromal cells on 3d collagen-hydroxyapatite scaffolds
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Sotirios Korossis, Thaqif El Khassawna, Alexander Y. Petrenko, Willem F. Wolkers, Birgit Glasmacher, Michael Gelinsky, Daniele Dipresa, Oleksandr Gryshkov, Ramon Cabiscol, Anja Lode, Vitalii Mutsenko, Bulat Sydykov, and Annemarie Beck
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03 medical and health sciences ,030219 obstetrics & reproductive medicine ,0302 clinical medicine ,Chemistry ,Mesenchymal stem cell ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,General Agricultural and Biological Sciences ,040201 dairy & animal science ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,Cell biology - Published
- 2018
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