37 results on '"Daniele Aparecida Matoso"'
Search Results
2. Impacts of Stress Caused by Copper Sulfate (CuSO4) on the Genome of the Tambaqui (Colossoma macropomum): Quantification of Rex1 and Heterochromatic Profile
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Hallana Cristina Menezes da Silva, Leila Braga Ribeiro, Adolfo José da Mota, Eliana Feldberg, and Daniele Aparecida Matoso
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transposable elements ,Real-Time PCR ,Copper sulfate. ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract The transposable elements are known by their ability to move and integrate into the genome of the host organism. They are classified in retrotransposons class I, which has, as intermediate of the transposition, the RNA and retrotransposons class II, which is composed of the DNA transposons, whose DNA migrates directly or is copied and inserted into the genome. The retrotransposable element Rex1 is a non-LTR retrotransposon found in several types of organisms. Many studies indicate that the Rex retrotransposons have the capacity to respond to environmental stress. The results found in this study corroborate the hypothesis that this retrotransposon possesses a response to environmental stress, since the chromosomal mapping, obtained through FISH, showed a higher number of markings on animals that were submitted to stress in 48h by the copper sulfate action. Furthermore, we can identify an increase in heterochromatic regions in the chromosomes. In the absolute quantification by Real-Time PCR, we found the largest numbers of Rex1 copies in the samples that were submitted to environmental stress by copper sulfate.
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- 2022
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3. A Perspective of Molecular Cytogenomics, Toxicology, and Epigenetics for the Increase of Heterochromatic Regions and Retrotransposable Elements in Tambaqui (Colossoma macropomum) Exposed to the Parasiticide Trichlorfon
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Maria dos Santos Costa, Hallana Cristina Menezes da Silva, Simone Cardoso Soares, Ramon Marin Favarato, Eliana Feldberg, Ana Lúcia Silva Gomes, Roberto Ferreira Artoni, and Daniele Aparecida Matoso
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FISH ,heterochromatinization ,organophosphate ,repetitive elements ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Rex retroelements are the best-known transposable elements class and are broadly distributed through fish and also individual genomes, playing an important role in their evolutionary dynamics. Several agents can stress these elements; among them, there are some parasitic compounds such as the organochlorophosphate Trichlorfon. Consequently, knowing that the organochlorophosphate Trichlorfon is indiscriminately used as an antiparasitic in aquaculture, the current study aimed to analyze the effects of this compound on the activation of the Transposable Elements (TEs) Rex1, Rex3, and Rex6 and the structure of heterochromatin in the mitotic chromosomes of the tambaqui (Colossoma macropomum). For this, two concentrations of the pesticide were used: 30% (0.261 mg/L) and 50% (0.435 mg/L) of the recommended LC50–96 h concentration (0.87 mg/L) for this fish species. The results revealed a dispersed distribution for Rex1 and Rex6 retroelements. Rex3 showed an increase in both marking intensity and distribution, as well as enhanced chromosomal heterochromatinization. This probably happened by the mediation of epigenetic adaptive mechanisms, causing the retroelement mobilization to be repressed. However, this behavior was most evident when Trichlorfon concentrations and exposure times were the greatest, reflecting the genetic flexibility necessary for this species to successfully adapt to environmental changes.
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- 2022
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4. Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes)
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Leila Braga Ribeiro, Daniele Aparecida Matoso, and Eliana Feldberg
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5S rDNA ,telomeric sequences ,tambaqui and pacu ,Genetics ,QH426-470 - Abstract
The Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade of the Serrasalmidae family: Colossoma macropomum, Mylossoma aureum, M. duriventre, and Piaractus mesopotamicus, in order to understand the chromosomal evolution in the family. All the species studied had diploid numbers 2n = 54 and exclusively biarmed chromosomes, but variations of the karyotypic formulas were observed. C-banding resulted in similar patterns among the analyzed species, with heterochromatic blocks mainly present in centromeric regions. The 18S rDNA mapping of C. macropomum and P. mesopotamicus revealed multiple sites of this gene; 5S rDNA sites were detected in two chromosome pairs in all species, although not all of them were homeologs. Hybridization with a telomeric probe revealed signals in the terminal portions of chromosomes in all the species and an interstitial signal was observed in one pair of C. macropomum.
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- 2014
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5. Karyotype variability in neotropical catfishes of the family Pimelodidae (Teleostei: Siluriformes)
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Américo Moraes Neto, Maelin da Silva, Daniele Aparecida Matoso, Marcelo Ricardo Vicari, Mara Cristina de Almeida, Maria João Collares-Pereira, and Roberto Ferreira Artoni
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Pseudoplatystoma reticulatum ,Pimelodus britskii ,Sorubim lima ,Steindachneridion parahybae ,Zoology ,QL1-991 - Abstract
Karyotypic data are presented for four species of fish belonging to the Pimelodidae family. These species show a conserved diploid number, 2n = 56 chromosomes, with different karyotypic formulae. The analyzed species showed little amount of heterochromatin located preferentially in the centromeric and telomeric regions of some chromosomes. The nucleolus organizer regions activity (Ag-NORs) and the chromosomal location of ribosomal genes by fluorescent in situ hybridization (FISH), with 18S and 5S probes, showing only one chromosome pair marked bearer of ribosomal genes, the only exception was Pimelodus britskii that presented multiple NORs and syntenic location of the 18S and 5S probes. Non-Robertsonian events, as pericentric inversion and NORs duplication are requested to explain the karyotype diversification in Pseudoplatystoma from the rio Paraguay (MS), Pimelodus from the rio Iguaçu (PR), Sorubim from the rio Paraguay (MS) and Steindachneridion from the rio Paraíba do Sul (SP). The obtained data for the karyotype macrostructure of these species corroborates a conserved pattern observed in Pimelodidae. On the other hand, interspecific variations detected by molecular cytogenetics markers made possible cytotaxonomic inferences and differentiation of the species here analyzed.Dados cariotípicos são apresentados para quatro espécies da família Pimelodidae. Todas apresentaram o mesmo número diploide, 2n = 56 cromossomos, com diferenças nas fórmulas cariotípicas. As espécies aqui analisadas mostraram pouca quantidade de heterocromatina localizada preferencialmente na região centromérica e telomérica de alguns cromossosmos do complemento cariotípico. As regiões organizadoras de nucléolo (Ag-RONs) e a localização dos genes ribossomais pela hibridização in situ fluorescente (FISH), com sondas 18S e 5S, evidenciaram somente um par cromossômico marcado portador de genes ribossomais, à exceção de Pimelodus britskii que apresentou NORs múltiplas e localização sintênica das sondas 18S e 5S. Eventos não-Robertsonianos, como inversão pericêntrica e duplicação das NORs são requeridos para explicar a diversificação cariotípica em Pseudoplatystoma do rio Paraguai (MS), Pimelodus do rio Iguaçu (PR), Sorubim do rio Paraguai (MS) e Steindachneridion do rio Paraíba do Sul (SP). Os dados obtidos para a macroestrutura cariotípica destas espécies corrobora um padrão conservado observado na família Pimelodidae. Por outro lado, evidências de variações interespecíficas pelos marcadores de citogenética molecular empregados possibilitam inferências citotaxonômicas e diferenciação das espécies aqui analisadas.
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- 2011
6. Preliminary qualitative analysis on mtDNA in Astyanax fasciatus populations Cuvier, 1819 (Teleostei; Characidae) indicate population distinctiveness
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Daniele Aparecida Matoso, Cesar Martins, Roberto Ferreira Artoni, and Pedro Manoel Galetti Jr
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Neotropical fish ,RFLP ,conservation genetics ,Biotechnology ,TP248.13-248.65 - Abstract
A preliminary qualitative analysis of genetic variability status in Astyanax fasciatus (Cuvier, 1819) from upper Tibagi River headwaters and Vila Velha State Park (VVSP) was carried out by enzymatic digestion (RFLP) of D-Loop region from mtDNA. The results showed that Tibagi and VVSP populations were genetically different.Uma análise qualitativa da variabilidade genética em Astyanax fasciatus (Cuvier, 1819) do alto Rio Tibagi e Parque Estadual de Vila Velha (PEVV) foi conduzida por digestão enzimática (RFLP) da região D-Loop do mtDNA. Os resultados evidenciaram que essas populações são geneticamente diferentes.
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- 2010
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7. Karyotype variability in neotropical catfishes of the family Pimelodidae (Teleostei: Siluriformes)
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Américo Moraes Neto, Maelin da Silva, Daniele Aparecida Matoso, Marcelo Ricardo Vicari, Mara Cristina de Almeida, Maria João Collares-Pereira, and Roberto Ferreira Artoni
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Pseudoplatystoma reticulatum ,Pimelodus britskii ,Sorubim lima ,Steindachneridion parahybae ,Zoology ,QL1-991 - Abstract
Karyotypic data are presented for four species of fish belonging to the Pimelodidae family. These species show a conserved diploid number, 2n = 56 chromosomes, with different karyotypic formulae. The analyzed species showed little amount of heterochromatin located preferentially in the centromeric and telomeric regions of some chromosomes. The nucleolus organizer regions activity (Ag-NORs) and the chromosomal location of ribosomal genes by fluorescent in situ hybridization (FISH), with 18S and 5S probes, showing only one chromosome pair marked bearer of ribosomal genes, the only exception was Pimelodus britskii that presented multiple NORs and syntenic location of the 18S and 5S probes. Non-Robertsonian events, as pericentric inversion and NORs duplication are requested to explain the karyotype diversification in Pseudoplatystoma from the rio Paraguay (MS), Pimelodus from the rio Iguaçu (PR), Sorubim from the rio Paraguay (MS) and Steindachneridion from the rio Paraíba do Sul (SP). The obtained data for the karyotype macrostructure of these species corroborates a conserved pattern observed in Pimelodidae. On the other hand, interspecific variations detected by molecular cytogenetics markers made possible cytotaxonomic inferences and differentiation of the species here analyzed.
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8. Methylation Profile of 18S rDNA Gene in Brain and Muscle of Tambaqui Exposed to Parasiticide Trichlorfon
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Daniele Aparecida Matoso
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General Medicine - Abstract
Until recently, there were no reports on the methylation profile of Amazonian fish specimens employing restriction enzymes. The 18S rDNA methylation status of samples of captive tambaqui (Colossoma macropomum) exposed to the antiparasitic Trichlorfon was examined in the current study. The method performed makes use of the CCGG site's sensitivity to the presence of methylation in palindrome cytosines for the restriction enzymes HpaII and MspI. The cleavage occurs by the detection or non-detection of the methyl radical in the inner (C5mCGG) or outer (5mCCGG) cytosine, which makes it possible to compare the DNA methylation profile. When compared to the specimens examined as the control group, the results obtained revealed an increase in methylated fragments of the 18S rDNA gene at LC concentrations of 30% and 50%.
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- 2023
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9. Impact of trichlorfon Organophosphate use in Pisciculture: a review
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Hallana Cristina Menezes da Silva, Ana Paula Costa de Carvalho, Ana Lúcia Silva Gomes, Roberto Ferreira Artoni, and Daniele Aparecida Matoso
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Classified as a class II organophosphate and considered highly toxic, trichlorfon is a drug widely used in worldwide pisciculture for the treatment of parasites in farming ponds. It is used for the treatment of several fish species, both in natural and artificial ponds. The main action of trichlorfon occurs in the nervous system of fish by blocking the enzyme acetylcholinesterase and causing acetylcholine accumulation, which leads to the constant passage of neural transmission. However, several studies show that trichlorfon can be more harmful to the fish than to the parasite that the drug is trying to eliminate. Our article brings a review of the main effects of trichlorfon in several fish species around the world, in order to further evaluate these side effects and help researchers to understand this drug
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- 2022
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10. Molecular diagnostic based on 18S rDNA and supplemental taxonomic data of the cnidarian coelozoic Ceratomyxa (Cnidaria, Myxosporea) and comments on the intraspecific morphological variation
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André C. Morandini, Luis Ramiro Luna Espinoza, Daniele Aparecida Matoso, Anai P. Flores-Gonzales, Fabrício Barros de Sousa, Patrick D. Mathews, Tiago Milanin, and Ana Lúcia Silva Gomes
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Cnidaria ,QH301-705.5 ,030231 tropical medicine ,Morphological variation ,Zoology ,Ceratomyxidae ,Intraspecific competition ,Myxosporea ,030308 mycology & parasitology ,ceratomyxid ,03 medical and health sciences ,0302 clinical medicine ,Animalia ,parasitic cnidarian ,18s rdna ,Ceratomyxa ,Myxozoa ,Biology (General) ,Ecology, Evolution, Behavior and Systematics ,0303 health sciences ,Bivalvulida ,biology ,ornamental fish ,biology.organism_classification ,CNIDARIA ,morphological plasticity ,Brazil - Abstract
Ceratomyxa amazonensis is a cnidarian myxosporean originally described with strongly arcuate crescent-shaped myxospores, absence of vegetative stages and infecting Symphysodon discus, an important Amazonian ornamental fish in the aquarium industry. As part of a long-term investigation concerning myxosporeans that infect discus fish Symphysodon spp. from different rivers of the Amazon Basin, thirty specimens of S. discus collected from Unini River were examined. Plasmodial vegetative stages therefrom were found freely floating in the bile of gall bladders from eighteen fish. Mature myxospores were slightly crescent-shaped, measuring 4.72 ± 0.1 (4.52–4.81) μm in length, 24.2 ± 0.4 (23.9–25.3) μm in thickness with polar capsules 2.31 ± 0.1 (2.29–2.33) μm in length and 2.15 ± 0.1 (2.13–2.17) μm in width. Strong morphological differences were observed between the newly isolated myxospores obtained and the previously described C. amazonensis; however, molecular assessment, based on 18S rDNA, revealed a high similarity (99.91%), with only a single nucleotide base change. This study provides new data, expanding the original description of the species with a discussion on differences in myxospore-morphology in the context of intraspecific morphological plasticity.
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- 2021
11. Acute toxicity of the pesticide trichlorfon and inhibition of acetylcholinesterase in Colossoma macropomum (Characiformes: Serrasalmidae)
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W. Duncan, Rebeca Fontenele Moda, Ana Lúcia Silva Gomes, Jhonatan Junior Silva Idalino, André Gentil da Silva, Daniele Aparecida Matoso, and Hallana Cristina Menezes da Silva
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Aché ,Glutathione ,Aquatic Science ,Pharmacology ,Pesticide ,Acetylcholinesterase ,language.human_language ,Acute toxicity ,chemistry.chemical_compound ,chemistry ,Toxicity ,language ,Uncompetitive inhibitor ,Agronomy and Crop Science ,IC50 - Abstract
In Brazilian fish farms, trichlorfon has been widely used to control acanthocephalan infections in Colossoma macropomum. Toxicity tests were conducted to estimate the median lethal concentration (LC50–96 h) and evaluate the effects of trichlorfon on acetylcholinesterase (AChE) and glutathione S-transferase (GST) from different tissues of C. macropomum. The LC50–96 h of trichlorfon was estimated to be 0.87 mg L−1. In the sublethal toxicity tests, concentrations of 0.26 mg L−1 (30% of LC50–96 h) and 0.43 mg L−1 (50% of LC50–96 h) were used. AChE and GST activities were measured in the brain, muscle, intestine, and liver. In vitro studies were conducted to estimate the kinetic properties and half-maximal inhibitory concentration (IC50) values of AChE in the brain and muscle for trichlorfon. In the sublethal toxicity experiments, inhibition of more than 90% of AChE in the brain, muscle, and intestine was observed. However, the activity of GST did not vary in any of the tissues studied. This finding suggests that trichlorfon is not metabolised by this enzyme. The in vitro assay results suggest that trichlorfon tends to be a classic uncompetitive inhibitor of AChE in both the brain and muscle, since Km and Vmax values decrease, while the slope remains unchanged. The IC50 values of muscle AChE are lower than those of the brain. All these results show that C. macropomum has low tolerance to this pesticide and suggest that brain AChE can be used as a biochemical biomarker, while muscle AChE may be used as an indicator of mortality in toxicological studies.
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- 2019
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12. Trichlorfon acute lethal toxicity to juvenile tambaqui ( Colossoma macropomum )
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W. Duncan, Daniele Aparecida Matoso, Jhonatan Junior Silva Idalino, Fabrício Barros de Sousa, Hallana Cristina Menezes da Silva, André Gentil da Silva, and Ana Lúcia Silva Gomes
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biology ,Tambaqui ,Toxicity ,Juvenile ,Zoology ,Aquatic Science ,biology.organism_classification - Published
- 2019
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13. Karyotypic Diversity and Evolutionary Trends in Neotropical Electric Fish of the Genus Gymnotus (Gymnotiformes: Gymnotidae)
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Daniele Aparecida Matoso, Maelin da Silva, Roberto Ferreira Artoni, and Eliana Feldberg
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biology ,Amazon rainforest ,Genus Gymnotus ,Zoology ,Gymnotiformes ,Family Gymnotidae ,Animal Science and Zoology ,Gymnotidae ,biology.organism_classification ,Electric fish ,Developmental Biology ,Order Gymnotiformes - Abstract
Electric fish of the order Gymnotiformes are endemic to the Neotropical region, and their highest diversity is observed in the Amazon region. The family Gymnotidae, which consists of the g...
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- 2019
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14. Composition and Nature of Heterochromatin in the Electrical Fish (Knifefishes) Gymnotus (Gymnotiformes: Gymnotidae)
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Roberto Ferreira Artoni, Eliana Feldberg, Daniele Aparecida Matoso, Maelin da Silva, and Vladimir Pavan Margarido
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0106 biological sciences ,0301 basic medicine ,biology ,Heterochromatin ,Gymnotiformes ,Zoology ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,030104 developmental biology ,%22">Fish ,Gymnotidae ,Composition (visual arts) ,Gymnotus - Abstract
Fishes of the genus Gymnotus have been suggested as a good model for biogeographic studies in the South American continent. In relation to heterochromatin, species of this genus have blocks preferably distributed in the centromeric region. The content of these regions has been shown to be variable, with description of transposable elements, pseudogenes of 5S rDNA and satellite sequences. In G. carapo Clade, although geographically separated, species with 2n = 54 chromosomes share the distribution of many 5S rDNA sites, a unique case within the genus. Here, repetitive DNA sequences from G. sylvius (2n = 40) and G. paraguensis (2n = 54) were isolated and mapped to understand their constitution. The chromosome mapping by FISH showed an exclusive association in the centromeres of all chromosomes. However, the cross-FISH did not show positive signs of interspecific hybridization, indicating high levels of heterochromatic sequence specificity. In addition, COI-1 sequences were analyzed in some species of Gymnotus, which revealed a close relationship between species of clade 2n = 54, which have multiple 5S rDNA sites. Possibly, the insertion of retroelements or pseudogenization and dispersion of this sequence occurred before the geographic dispersion of the ancestor of this clade from the Amazon region to the hydrographic systems of Paraná-Paraguay, a synapomorphy for the group.
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- 2021
15. Chromosomal Evolution in Aspredinidae (Teleostei, Siluriformes): Insights on Intra- and Interspecific Relationships with Related Groups
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Eliana Feldberg, Caroline Garcia, Isac Silva de Jesus, Patrik F. Viana, Milena Ferreira, Luiz Antonio Carlos Bertollo, Daniele Aparecida Matoso, and Marcelo de Bello Cioffi
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Aspredinidae ,Male ,DNA, Ribosomal ,Chromosomes ,Evolution, Molecular ,Monophyly ,Species Specificity ,Sequence Homology, Nucleic Acid ,Genetics ,RNA, Ribosomal, 18S ,Animals ,DNA Barcoding, Taxonomic ,Clade ,Molecular Biology ,Genetics (clinical) ,Catfishes ,In Situ Hybridization, Fluorescence ,Phylogeny ,Cytotaxonomy ,Sex Chromosomes ,biology ,RNA, Ribosomal, 5S ,Bunocephalus ,Karyotype ,Sequence Analysis, DNA ,biology.organism_classification ,Biological Evolution ,Diploidy ,Evolutionary biology ,Karyotyping ,Female ,Ploidy ,Amaralia hypsiura ,Sequence Alignment ,Brazil - Abstract
The family Aspredinidae comprises a clade of complex systematic relationships, both from molecular and morphological approaches. In this study, conventional and molecular cytogenetic studies coupled with nucleotide sequencing were performed in 6 Aspredininae species (Amaralia hypsiura, Bunocephalus cf. aloikae, Bunocephalus amaurus, Bunocephalus aff. coracoideus, Bunocephalus verrucosus, and Platystacus cotylephorus) from different locations of the Amazon hydrographic basin. Our results showed highly divergent diploid numbers (2n) among the species, ranging from 49 to 74, including the occurrence of an XX/X0 sex chromosome system. A neighbor-joining phylogram based on the cytochrome c oxidase I (COI) showed that Bunocephalus coracoideus is not a monophyletic clade, but closely related to B. verrucosus. The karyotypic data associated with COI suggest an ancestral karyotype for Aspredinidae with a reduced 2n, composed of bi-armed chromosomes and a trend toward chromosomal fissions resulting in higher diploid number karyotypes, mainly composed of acrocentric chromosomes. Evolutionary relationships were discussed under a phylogenetic context with related species from different Siluriformes families. The karyotype features and chromosomal diversity of Aspredinidae show an amazing differentiation, making this family a remarkable model for investigating the evolutionary dynamics in siluriforms as well as in fish as a whole.
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- 2020
16. Chromosomal Mapping of Rex Retrotransposons in Tambaqui (Colossoma macropomum Cuvier, 1818) Exposed to Three Climate Change Scenarios
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Alex M.V. Ferreira, Daniele Aparecida Matoso, Eliana Feldberg, Leandro Marajó, and Leila Braga Ribeiro
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0106 biological sciences ,0303 health sciences ,biology ,Heterochromatin ,Tambaqui ,Chromosome ,Zoology ,Climate change ,Retrotransposon ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,Greenhouse gas ,Genetics ,Gradual increase ,Microcosm ,Molecular Biology ,Genetics (clinical) ,030304 developmental biology - Abstract
Greenhouse gas emissions are known to influence the planet's temperature, mainly due to human activities. To allow hypothesis testing, as well as to seek viable alternatives for mitigation, the Intergovernmental Panel on Climate Change (IPCC) suggested 3 main scenarios for changes projected for the year 2100. In this paper, we subjected Colossoma macropomum Cuvier, 1818 (tambaqui) individuals in a microcosm to IPCC scenarios B1 (mild), A1B (intermediate), and A2 (extreme) to test possible impacts on their genome. We found chromosome heterochromatinization in specimens exposed to the A2 scenario, where terminal blocks and interstitial bands were detected on several chromosome pairs. The behavior of Rex1 and Rex3 sequences differed between the test scenarios. Hybridization of Rex1 resulted in diffuse signals which showed a gradual increase in the tested scenarios. For Rex3, an increase was observed in the A2 scenario with blocks on several chromosomes, some of which coincided with heterochromatin. Heterochromatinization is an epigenetic process, which may have occurred as a mechanism for regulating Rex3 activity. The signal pattern of Rex6 did not change, suggesting that other mechanisms are acting to regulate its activity.
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- 2019
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17. Karyotypic Diversity and Evolutionary Trends in Neotropical Electric Fish of the Genus
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Maelin, da Silva, Daniele Aparecida, Matoso, Roberto Ferreira, Artoni, and Eliana, Feldberg
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Evolution, Molecular ,Species Specificity ,Karyotype ,Gymnotiformes ,Animals ,Biological Evolution ,DNA, Ribosomal ,Brazil ,Phylogeny - Abstract
Electric fish of the order Gymnotiformes are endemic to the Neotropical region, and their highest diversity is observed in the Amazon region. The family Gymnotidae, which consists of the genera
- Published
- 2019
18. A new multiple sex chromosome system X1X1X2X2/X1Y1X2Y2 in Siluriformes: cytogenetic characterization of Bunocephalus coracoideus (Aspredinidae)
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Isac Silva de Jesus, Caroline Garcia, Milena Ferreira, Eliana Feldberg, and Daniele Aparecida Matoso
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0106 biological sciences ,0301 basic medicine ,Genetics ,Chromosome 7 (human) ,Plant Science ,General Medicine ,Biology ,010603 evolutionary biology ,01 natural sciences ,Chromosome 17 (human) ,03 medical and health sciences ,030104 developmental biology ,Chromosome 4 ,Chromosome 16 ,Chromosome 3 ,Chromosome 18 ,Insect Science ,Chromosome 19 ,Animal Science and Zoology ,Chromosome 12 - Abstract
We analyzed one Bunocephalus coracoideus population from the Negro River basin using cytogenetic techniques. The results showed a diploid number of 42 chromosomes in both sexes, with the karyotypic formula 4m + 14sm + 24a and fundamental number (FN) = 60 for females and the formula 5m + 14sm + 23a and FN = 61 for males, constituting an X1X1X2X2/X1Y1X2Y2 multiple sex chromosome system. The constitutive heterochromatin is distributed in the pericentromeric regions of most of the chromosomes, except for the sex chromosomes, of which the X1, X2, and Y1 chromosomes were euchromatic and the Y2 chromosome was partially heterochromatic. 18S rDNA mapping confirmed the presence of nucleolar organizer regions on the short arms of the fifth chromosomal pair for both sexes. The 5S rDNA is present in the terminal regions of the short arms on the 2nd, 10th, and 12th pairs and on the X2 chromosome of both sexes; however, we observed variations in the presence of these ribosomal cistrons on the Y1 chromosome, on which the cistrons are pericentromeric, and on the Y2 chromosome, on which these cistrons are present in the terminal portions of the short and long arms. Telomeric sequences are located in the terminal regions of all of the chromosomes, particularly conspicuous blocks on the 10th and 12th pairs and internal telomeric sequences in the centromeric regions of the 1st, 6th, and 9th pairs for both sexes. This work describes an new sex chromosomes system for the Siluriformes and increases our genetic knowledge of the Aspredinidae family.
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- 2016
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19. Genetic structuring among silverside fish (Atherinella brasiliensis) populations from different Brazilian regions
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Marta Margarete Cestari, Maíra Carneiro Proietti, Ana Luzia de Figueiredo Lacerda, Pedro Fernandes Sanmartin Prata, Ralf Kersanach, Luiz Felipe Cestari Dumont, Daniele Aparecida Matoso, Maria Cristina da Silva Cortinhas, Fernando D'Incao, T. A. Boni, Wanessa Algarte Ramsdorf, Alberto José Prioli, and Rafael Bueno Noleto
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0106 biological sciences ,0301 basic medicine ,Mitochondrial DNA ,Genetic diversity ,education.field_of_study ,Ecology ,media_common.quotation_subject ,Haplotype ,Population ,Aquatic Science ,Biology ,Oceanography ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,03 medical and health sciences ,Speciation ,030104 developmental biology ,Genetic variability ,Neotropical silversides ,education ,media_common ,Isolation by distance - Abstract
Estuaries are dynamic environments, key for the survival of innumerous ecologically or economically important fish species. Among these species are Neotropical silversides (Atherinella brasiliensis), which are resident and abundant in Brazilian estuaries and used as a complementary source of income and food for local communities. To better understand silverside populations in Brazil, we evaluated the genetic diversity, structure and demography of fish sampled at six estuaries from the northeastern to the southern coast, using Random Amplified Polymorphic DNA and mitochondrial DNA (D-loop) markers. High haplotype diversities (h ranging from 0.75 to 0.99) were found in all populations except Carapebus, located in Southeast Brazil (h = 0.54). A total of 69 mtDNA haplotypes were found, with Itaparica (Northeast Brazil) and Carapebus presenting only exclusive haplotypes, while some were shared among populations in the South. Strong regional structure was observed, with very high differentiation between Itaparica and Carapebus, as well as among these two populations and the ones from the Southern region (Paranagua, Conceicao, Camacho and Patos). Among southern areas, low/moderate structure was detected. Most populations showed unimodal mismatch distributions indicating recent demographic expansion, while Carapebus presented a multimodal distribution characteristic of a stable or bottlenecked population. Times since possible population expansion were highest in Itaparica (32,500 ya) and Carapebus (29,540 ya), while in the Southern region longest time was observed at Conceicao (25,540 ya) and shortest at Patos (9720 ya). In a general manner, haplotype diversities were directly related to times since population expansions; again, Carapebus was the exception, displaying long time since expansion but low diversity, possibly due to a recent bottleneck caused by the isolation and human impacts this lagoon is subject to. Isolation by Distance was significant for Itaparica and Carapebus, and considering the extremely high differentiation of these populations, we suggest that they could be undergoing speciation. To adequately manage and maintain the genetic variability of silversides in Brazilian estuaries, we propose three distinct management units for this species: 1) Itaparica; 2) Carapebus; and 3) Southern Brazil.
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- 2016
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20. New Approach Data in Electric Fish (Teleostei: Gymnotus): Sex Chromosome Evolution and Repetitive DNA
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Eliana Feldberg, Daniele Aparecida Matoso, Roberto Ferreira Artoni, and Maelin da Silva
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Genetics ,Teleostei ,Sex Chromosomes ,Models, Genetic ,biology ,Gymnotiformes ,Chromosome ,Karyotype ,biology.organism_classification ,Evolution, Molecular ,Species Specificity ,Evolutionary biology ,Phylogenetics ,Karyotyping ,Sexual selection ,Animals ,Animal Science and Zoology ,Gymnotidae ,Gymnotus ,Clade ,In Situ Hybridization, Fluorescence ,Repetitive Sequences, Nucleic Acid ,Developmental Biology - Abstract
Antagonist sexual selection is the driving force behind the origin and diversification of sex chromosomes such as XX/XY and ZZ/ZW. However, chromosome mobility, mainly in fishes, may result in the formation of chromosomes of recent origin, a process known as turnover. The family Gymnotidae, which is composed of the genera Electrophorus+Gymnotus, presents a multiple system of the type X1X1X2X2/X1X2Y, which has been described for Gymnotus pantanal. This article describes the karyotype of three Amazon Gymnotus species, revealing the presence of both simple and multiple systems: Gymnotus carapo "Catalão" 2n=40 XX/XY, Gymnotus coropinae 2n=49♂/50♀ X1X1X2X2/X1X2Y, and Gymnotus sp. "Negro" 2n=50 XX/XY. Our hypothesis is that the simple system present in G. carapo "Catalão" is ancestral in relation to G. pantanal's multiple system and that the diversification of the subsequent multiple system occurred after the final separation of the Amazon and Paraná basins. Moreover, G. coropinae's multiple system may have originated from the simple system present in Gymnotus sp. "Negro." The distant position between the species in the Gymnotidae family's phylogeny in addition to differences in sex chromosome formula and number between Clade G1 G. coropinae and G. sp. "Negro" species and "Carapo" Clade. G. carapo and G. pantanal species suggest that both sequences of sexual systems occurred independently, supporting other proposed models and highlighting the fact that species of the genus Gymnotus may serve as a model for studying sex chromosome turnover.
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- 2014
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21. An HpaII/MspI-PCR assay to measure methylation of DNA in Hoplosternum littorale (Callichthyidae, Siluriformes) from a polluted environment in the central Amazon basin
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Carlos Henrique Schneider, Francijara Araújo da Silva, Maria Claudia Gross, Gislene Almeida Carvalho-Zilse, Daniele Aparecida Matoso, Leonardo Gusso Goll, and Eliana Feldberg
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0106 biological sciences ,0301 basic medicine ,Genetics ,Transposable element ,HpaII ,Soil Science ,Plant Science ,Methylation ,Biology ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,DNA sequencing ,03 medical and health sciences ,Restriction enzyme ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,DNA methylation ,Hoplosternum littorale ,DNA ,General Environmental Science - Abstract
The HpaII/MspI-PCR is a method based on the sensitivity of restriction enzymes to methylation at their cleavage sites, which permits the comparison of the DNA methylation status of different organisms, based on the differential patterns of digestion. Prior to the present study, nothing was known of the methylation profile of specific DNA sequences in Amazonian fish based on enzymes sensitive to methylation. Given this, the present study investigated the methylation profile of the 18S rRNA gene of the armored catfish, Hoplosternum littorale, which inhabits relatively inhospitable environments in the Amazon basin. A differential methylation profile was found in the 18S rRNA sites, indicating that polluted environments may influence the methylation of DNA, and would have evolved in the species, initially, as a defense mechanism against the expression of transposable elements.
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- 2019
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22. The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity
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Jansen Zuanon, Eliana Feldberg, Isac Silva de Jesus, Caroline Garcia, Marcelo de Bello Cioffi, Luiz Antonio Carlos Bertollo, Milena Ferreira, and Daniele Aparecida Matoso
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0106 biological sciences ,0301 basic medicine ,Image Processing ,01 natural sciences ,Polymerase Chain Reaction ,ecological adaptations ,Monophyly ,Chromosome Analysis ,Ribosome Dna ,Testis ,chromosomal differentiation ,Clade ,Rna 5s ,Genetics (clinical) ,In Situ Hybridization, Fluorescence ,Phylogeny ,Original Research ,Genetics ,education.field_of_study ,Biodiversity ,Bunocephalus Coracoideus ,banjo catfish ,Genetic Variability ,Sympatric speciation ,Molecular Medicine ,Dna Sequence ,Species complex ,lcsh:QH426-470 ,Evolution ,Population ,Allopatric speciation ,Catfish ,Biology ,010603 evolutionary biology ,Dna 18s ,03 medical and health sciences ,Cytogenetics ,education ,evolutionary units ,Genetic diversity ,Dna Barcoding ,Gene Amplification ,biology.organism_classification ,Nonhuman ,Dna Denaturation ,lcsh:Genetics ,030104 developmental biology ,Evolutionary biology ,Kidney Cell ,molecular taxonomy ,Bunocephalus coracoideus - Abstract
Bunocephalus is the most species-rich Aspredinidae genus, corresponding to a monophyletic clade with 13 valid species. However, many species have their classification put in question. Here, we analyzed individuals from four Amazonian populations of Bunocephalus coracoideus by cytogenetic and molecular procedures. The geographic distribution, genetic distances and karyotype data indicate that each population represents an Evolutionary Significant Unit (ESU). Cytogenetic markers showed distinct 2n and karyotype formulas, as well as different numbers and locations of the rDNA sites among ESUs. One of such populations (ESU-D) highlighted an extensive polymorphic condition, with several cytotypes probably due to chromosomal rearrangements and meiotic non-disjunctions. This resulted in several aneuploid karyotypes, which was also supported by the mapping of telomeric sequences. Phylograms based on Maximum Likelihood (ML) and Neighbor Joining (NJ) analyses grouped each ESU on particular highly supported clades, with the estimation of evolutionary divergence indicating values being higher than 3.8-12.3% among them. Our study reveals a huge degree of chromosomal and genetic diversity in B. coracoideus and highly points to the existence of four ESUs in allopatric and sympatric speciation processes. In fact, the high divergences found among the ESUs allowed us to delimitate lineages with taxonomic uncertainties in this nominal species. © 2017 Ferreira, Garcia, Matoso, de Jesus, Cioffi, Bertollo, Zuanon and Feldberg.
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- 2017
23. Fluorescence Image Analyzer - FLIMA: software for quantitative analysis of fluorescence in situ hybridization
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M M C Martins-Júnior, H C M Silva, L B Ribeiro, and Daniele Aparecida Matoso
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Spectrum analyzer ,Materials science ,Image Processing ,Biotin ,Color ,Image processing ,Image Analysis ,Chromosome ,Procedures ,Chromosomes ,Evaluation Studies As Topic ,Evaluation Study ,chemistry.chemical_compound ,Microscopy ,Image Processing, Computer-Assisted ,Genetics ,Fluorescence microscope ,medicine ,Digoxigenin ,Controlled Study ,Image Processing, Computer-assisted ,Molecular Biology ,In Situ Hybridization, Fluorescence ,Pixel ,medicine.diagnostic_test ,Quantitative Analysis ,Nucleic Acid Hybridization ,General Medicine ,Fluorescence ,Algorithm ,Microscopy, Fluorescence ,chemistry ,Evaluation Studies as Topic ,Biological system ,Software ,Fluorescence in situ hybridization - Abstract
The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed. © 2017 The Authors.
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- 2017
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24. Chromosomal Mapping of Transposable Elements of the Rex Family in the Bristlenose Catfish, Ancistrus (Siluriformes, Loricariidae), from the Amazonian Region
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Eliana Feldberg, Ramon Marin Favarato, Daniele Aparecida Matoso, and Leila Braga Ribeiro
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0301 basic medicine ,Male ,Gene Cluster ,Retrotransposon ,Repetitive Dna ,Genome ,Chromosome 10 ,Euchromatin ,Ancistrus dolichopterus ,Heterochromatin ,Ancistrus Dolichopterus ,Genetics (clinical) ,Rex6 Protein ,Catfishes ,In Situ Hybridization, Fluorescence ,biology ,Ancistrus Ranunculus ,Rex Protein ,Ancistrus Sp. Catalao ,Chromosome 18 ,Species Distribution ,Chromosome Mapping ,Chromosome 19 ,Chromosome 16 ,Chromosome 17 ,Classification ,Chromosome 12 ,Chromosome 13 ,Chromosome 21 ,Chromosome 20 ,Fish Protein ,Conservation Genetics ,Priority Journal ,Female ,Animals Experiment ,Brazil ,Biotechnology ,Catfish ,Retroelements ,Loricariidae ,Karyotype ,Chromosomal Mapping ,Ancistrus Sp. Purus ,Fish Genetics ,Chromosome 25 ,Chromosome 26 ,Chromosome 24 ,Evolution, Molecular ,03 medical and health sciences ,Unclassified Drug ,Cytogenetics ,Dna Transposable Elements ,Animals Tissue ,Autosome ,Genetics ,Protein Family ,Animals ,Molecular Biology ,Transposon ,Ancistrus Aff. Dolichopterus ,Chromosome 9 ,Chromosome 7 ,Chromosome 8 ,Animal ,Brasil ,Retroposon ,Chromosome ,Chromosome 1 ,Genetic Variation ,Ancistrus ,Ancistrus Dubius ,Chromosome 2 ,biology.organism_classification ,Rex3 Protein ,Chromosome 5 ,Nonhuman ,Chromosome 6 ,Chromosome 3 ,Chromosome 4 ,Fishery ,Ancistrus Maximus ,030104 developmental biology ,Evolutionary biology ,Rex1 Protein ,DNA Transposable Elements ,Siluriformes - Abstract
Repetitive DNA sequences are present in the genome of basically every known organism, and transposable elements (TE) are one of the most representative sequences involved in chromosomal rearrangements and the genomic evolution of eukaryotes. In fish, the non-LTR retrotransposon TEs, Rex1, Rex3, and Rex6, are widely distributed in fish genomes and are the best-characterized TEs in several species. In the current study, three of these retroelements were physically mapped, through fluorescent in situ hybridization (FISH), in 7 species (71 specimens) of the genus Ancistrus, known as bristlenose catfish: Ancistrus ranunculus, Ancistrus sp. 1 Purus, Ancistrus sp. 2 Catalão, Ancistrus dolichopterus, Ancistrus maximus, Ancistrus aff. dolichopterus, and Ancistrus dubius. Rex1, Rex3, and Rex6 showed a cluster distribution, mainly in the terminal and pericentromeric portions, in heterochromatic and euchromatic regions, and did not occur in sexual chromosomes; however, the number and position of the clusters varied between species. This TE distribution suggests its implication in the karyotypic evolution of these species, without affecting the rise of sexual chromosome systems in Ancistrus, in view of their chromosomal variation. © The American Genetic Association 2016. All rights reserved.
- Published
- 2016
25. A new multiple sex chromosome system X
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Milena, Ferreira, Caroline, Garcia, Daniele Aparecida, Matoso, Isac Silva, de Jesus, and Eliana, Feldberg
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Male ,Cytogenetics ,Sex Chromosomes ,Heterochromatin ,Karyotype ,RNA, Ribosomal, 18S ,RNA, Ribosomal, 5S ,Animals ,Female ,Catfishes ,In Situ Hybridization, Fluorescence - Abstract
We analyzed one Bunocephalus coracoideus population from the Negro River basin using cytogenetic techniques. The results showed a diploid number of 42 chromosomes in both sexes, with the karyotypic formula 4m + 14sm + 24a and fundamental number (FN) = 60 for females and the formula 5m + 14sm + 23a and FN = 61 for males, constituting an X
- Published
- 2016
26. Chromosomal polymorphism in Steindachneridion melanodermatum Garavello, 2005 (Siluriformes, Pimelodidae): a reappraisal the existence of sex chromosome system in the species
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Vera Maria Fonseca de Almeida Val, Roberto Ferreira Artoni, Marcelo Ricardo Vicari, Mara Cristina de Almeida, Daniele Aparecida Matoso, Orlando Moreira-Filho, Américo Moraes-Neto, and Maelin da Silva
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Genetics ,Pimelodidae ,Sexual differentiation ,Physical Chromosome Mapping ,Chromosomal polymorphism ,Chromosome ,Karyotype ,Aquatic Science ,Nucleolus organizer region ,Biology ,Repeated sequence ,biology.organism_classification - Abstract
Fifty-five specimens of Steindachneridion melanodermatum were analyzed using molecular and conventional cytogenetic tools. Two polymorphisms were found: one involving the length of nucleolar organizer regions and another involving two submetacentric chromosomes previously identified as sex chromosomes. The polymorphism was confirmed by homogeneity between male and female karyotypes. Nucleotide sequencing and physical chromosome mapping were also used to identify and characterize one class of repetitive DNA, named SmAluI-Rex3. Based on the results and literature the present study offers an update of the occurrence of sex chromosome system in this species.
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- 2011
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27. Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil
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Maelin da Silva, Daniele Aparecida Matoso, Marcelo Ricardo Vicari, Mara Cristina de Almeida, Eder Gomes, Roberto Ferreira Artoni, and Luis Augusto M. Ludwig
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Conservation genetics ,medicine.medical_specialty ,fungi ,Cytogenetics ,Zoology ,Aquatic Science ,Biology ,Ribosomal RNA ,Rhamdia ,biology.organism_classification ,Genetic marker ,Neotropical fish ,medicine ,Ploidy ,Ecology, Evolution, Behavior and Systematics ,Catfish - Abstract
The freshwater silver catfish from the genus Rhamdia belongs to a group of neotropical fish with wide distribution throughout Central and South America. The results obtained in the F1 generation of native specimens of this species bred in captivity using molecular cytogenetic tools revealed a high number of triploid specimens (46.8%) in the samples analyzed. The use of double fluorescent in situ hybridization (double-FISH) with 18S and 5S rDNA probes allowed highlighting the location of ribosomal sites with independent activity. This chromosome marker allowed the identification of triploid nuclei, in which three markers were visualized, in contrast to diploid fish, which only exhibited two fluorescent signals. As triploid specimens exhibit either total or partial gonad sterility, the present study demonstrates the importance of cytogenetic monitoring in restocking programs aimed at genetic conservation.
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- 2011
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28. Physical Mapping of 5S rDNA in Two Species of Knifefishes: Gymnotus pantanal and Gymnotus paraguensis (Gymnotiformes)
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Daniele Aparecida Matoso, Vladimir Pavan Margarido, Marcelo Ricardo Vicari, R.F. Artoni, M.C. de Almeida, and M. da Silva
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Genetics ,Transposable element ,biology ,medicine.diagnostic_test ,Gymnotiformes ,Karyotype ,biology.organism_classification ,Genome ,Gene cluster ,medicine ,Gymnotus ,Molecular Biology ,Genetics (clinical) ,Fluorescence in situ hybridization ,Cytotaxonomy - Abstract
Physical mapping of 5S rDNA in 2 species of knifefishes, Gymnotuspantanal and G. paraguensis (Gymnotiformes), was performed using fluorescence in situ hybridization with a 5S rDNA probe. The 5S rDNA PCR product from the genomes of both species was also sequenced and aligned to determine non-transcribed spacer sequences (NTS). Both species under study had different patterns of 5S rDNA gene cluster distribution. While in the karyotype of G. pantanal two 5S rDNA-bearing pairs were observed, the karyotype of G. paraguensis possessed as many as 19 such pairs. Such multiplication of 5S rDNA gene clusters might be caused by the involvement of transposable elements because the NTS of G. paraguensis was 400 bp long with high identity (90%) with a mobile transposable element called Tc1-like transposon, described from the cyprinid fish Labeo rohita.
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- 2011
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29. Two genetic stocks of Steindachneridion melanodermatum living in sympatry in nature and genetic variability of wild parents and F1 generation
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M.C.S. Cortinhas, M.C. de Almeida, Daniele Aparecida Matoso, R.F. Artoni, M. da Silva, Marcelo Ricardo Vicari, and Marta Margarete Cestari
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Genetic Markers ,Sympatry ,Gene Mutation ,Captivity ,Zoology ,Catfish ,Biology ,Gene mutation ,Genetic Similarity ,Genetic Marker ,Genetic variation ,Genetics ,Animals ,Controlled Study ,Genetic variability ,Molecular Biology ,Catfishes ,River ,Heterozygosity ,Random Amplified Polymorphic Dna Technique ,Animal ,Brasil ,Genetic Variation ,General Medicine ,RAPD ,Gene Locus ,Genetic Variability ,Genetic Loci ,Genetic marker ,Random Amplified Polymorphic Dna - Abstract
Steindachneridion melanodermatum is a large Brazilian catfish, highly prized for sport fishing and for its meat. Specimens of this species, both caught in nature from Iguacu River and F1 fish born in captivity, were analyzed with regard to patterns of RAPD molecular markers. Genetic similarity ranged from 0.57 to 0.95; two groups were determined for the wild specimens. The results suggest different genetic lineages in sympatry in nature. Heterozygosity and percentage of polymorphic loci were 0.31 and 79% and 0.23 and 62%, respectively, for the two populations of wild specimens and 0.26 and 66%, respectively, for those born in captivity. © FUNPEC-RP.
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- 2011
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30. Preliminary qualitative analysis on mtDNA in Astyanax fasciatus populations Cuvier, 1819 (Teleostei; Characidae) indicate population distinctiveness
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Roberto Ferreira Artoni, Cesar Martins, Pedro M. Galetti, Daniele Aparecida Matoso, Universidade Federal do Paraná (UFPR), Universidade Estadual Paulista (Unesp), Universidade Estadual de Ponta Grossa (UEPG), and Universidade Federal de São Carlos (UFSCar)
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Conservation genetics ,education.field_of_study ,Teleostei ,Multidisciplinary ,biology ,lcsh:Biotechnology ,Population ,Zoology ,biology.organism_classification ,Neotropical fish ,Characidae ,conservation genetics ,lcsh:TP248.13-248.65 ,Genetic variation ,RFLP ,Genetic variability ,Restriction fragment length polymorphism ,education - Abstract
Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:41:00Z No. of bitstreams: 1 S1516-89132010000300022.pdf: 2246650 bytes, checksum: 0ab14aadc1325471194348d8a9d69f40 (MD5) Made available in DSpace on 2013-08-22T18:41:00Z (GMT). No. of bitstreams: 1 S1516-89132010000300022.pdf: 2246650 bytes, checksum: 0ab14aadc1325471194348d8a9d69f40 (MD5) Previous issue date: 2010-06-01 Made available in DSpace on 2013-09-30T19:27:14Z (GMT). No. of bitstreams: 2 S1516-89132010000300022.pdf: 2246650 bytes, checksum: 0ab14aadc1325471194348d8a9d69f40 (MD5) S1516-89132010000300022.pdf.txt: 12109 bytes, checksum: 2a7e4f6c5e4921934211864b31fbc263 (MD5) Previous issue date: 2010-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:07:34Z No. of bitstreams: 2 S1516-89132010000300022.pdf: 2246650 bytes, checksum: 0ab14aadc1325471194348d8a9d69f40 (MD5) S1516-89132010000300022.pdf.txt: 12109 bytes, checksum: 2a7e4f6c5e4921934211864b31fbc263 (MD5) Made available in DSpace on 2014-05-20T15:07:34Z (GMT). No. of bitstreams: 2 S1516-89132010000300022.pdf: 2246650 bytes, checksum: 0ab14aadc1325471194348d8a9d69f40 (MD5) S1516-89132010000300022.pdf.txt: 12109 bytes, checksum: 2a7e4f6c5e4921934211864b31fbc263 (MD5) Previous issue date: 2010-06-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Uma análise qualitativa da variabilidade genética em Astyanax fasciatus (Cuvier, 1819) do alto Rio Tibagi e Parque Estadual de Vila Velha (PEVV) foi conduzida por digestão enzimática (RFLP) da região D-Loop do mtDNA. Os resultados evidenciaram que essas populações são geneticamente diferentes. A preliminary qualitative analysis of genetic variability status in Astyanax fasciatus (Cuvier, 1819) from upper Tibagi River headwaters and Vila Velha State Park (VVSP) was carried out by enzymatic digestion (RFLP) of D-Loop region from mtDNA. The results showed that Tibagi and VVSP populations were genetically different. Universidade; Federal do Paraná Departamento de Genética Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de Morfologia Universidade Estadual de Ponta Grossa Departamento de Biologia Estrutural, Molecular e Genética Universidade Federal de São Carlos (UFSCar) Departamento de Genética e Evolução Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de Morfologia
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- 2010
31. A prime inference on genetic diversity (RAPDs) in the marine fishAtherinella brasiliensis(Teleostei, Atherinopsidae) from Southern Brazil
- Author
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Alberto José Prioli, Marta Margarete Cestari, Maria Cristina Da Silva Cortinhas, Daniele Aparecida Matoso, Chirlei Glienke, and Rafael Bueno Noleto
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Conservation genetics ,education.field_of_study ,Genetic diversity ,Ecology ,Population ,Zoology ,Cell Biology ,Biology ,Analysis of molecular variance ,RAPD ,Vicariance ,Mantel test ,Animal Science and Zoology ,Genetic variability ,education ,Ecology, Evolution, Behavior and Systematics - Abstract
Da Silva Cortinhas, M. C., Glienke, C., Prioli, A. J., Noleto, R. B., Matoso, D. A. and Cestari, M. M. 2010. A prime inference on genetic diversity (RAPDs) in the marine fish Atherinella brasiliensis (Teleostei, Atherinopsidae) from Southern Brazil. — Acta Zoologica (Stockholm) 91: 242-248 As a result of the importance of Atherinella brasiliensis in estuarine environ- ments, random amplified polymorphic DNA (RAPD) markers were used to verify the genetic diversity in A. brasiliensis from two different places in Paran- aguaBay (ParanaState) and one from the Conceicao Lagoon (Santa Catarina State). Cytogenetic data have shown a high karyotypic diversity in some popu- lations, although in others this peculiarity demonstrates rearrangements such as heterochromatinization. In the present study, a low level of genetic structuring between the samples from Conceicao Lagoon compared with the others was observed through principal coordinate analysis (PCO), analysis of molecular variance and Mantel test according to 79 RAPD markers. As this specie does not perform horizontal migration and the individuals of Conceicao Lagoon are isolated, three hypotheses are proposed to explain the results: (i) similar envi- ronments may show homogeneous populations not depending on the geo- graphical distance, (ii) because vicariant events that formed the bays occurred in a recent period, the fragmentation effects over the structuring of the genetic diversity may still be low and not totally detectable by the RAPD technique and (iii) the isolation time or the number of generations may not be enough to promote a possible differentiation and genetic structuring between the speci- mens of these three places. The specimens of these places present a low level of differentiation and genetic structuring so we can consider them as a unique homogeneous population.
- Published
- 2010
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32. Karyotypic variability in Iheringichthys labrosus (Teleostei, Pimelodidae) from the Tibagi River basin (Paraná State, Brazil)
- Author
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Mara Cristina de Almeida, Daniele Aparecida Matoso, Maria Carolina Costa Melo Svidnicki, A. Moraes-Neto, Marcelo Ricardo Vicari, L.B. Ribeiro, and R.F. Artoni
- Subjects
Male ,Pimelodidae ,Teleost ,Population ,Drainage basin ,Zoology ,Teleostei ,Iheringichthys Labrosus ,Chromosomes ,Chromosome Analysis ,Rivers ,Heterochromatin ,River Basin ,Genetics ,Animals ,Controlled Study ,education ,Molecular Biology ,Metaphase ,Catfishes ,geography ,B chromosome ,education.field_of_study ,geography.geographical_feature_category ,biology ,Supernumerary Chromosome ,Ecology ,Brasil ,Chromosome ,Karyotype ,General Medicine ,Nonhuman ,biology.organism_classification ,Iheringichthys labrosus ,Chromosome Pairing ,Karyotyping ,Female ,Brazil - Abstract
Cytogenetic analyses were carried out in a populational sample of Iheringichthys labrosus from the Guaraúna River (Upper Tibagi River; Paraná State, Brazil) in order to provide a karyotypic comparison with another previously studied population from the Lower Tibagi River, characterized by the presence of 32m + 8sm + 6st + 10a (2n = 56, FN = 102) and occurrence of supernumerary chromosomes (80% of individuals). The 17 specimens of I. labrosus (6 females, 10 males and 1 of unknown sex) from the Upper Tibagi River showed 2n = 56 chromosomes, a karyotype formula of 14m + 32sm + 4st + 6a (FN = 106), without evidence of sex chromosome heteromorphism or supernumerary chromosomes. The heterochromatin was detected at telomeric and centromeric positions in several chromosomal pairs. The Ag-nucleolar organizer regions were heteromorphic and located at terminal position on short arms of the 16th chromosomal pair, suggesting a positive association with heterochromatic regions. The inter-populational karyotypic differentiation reported indicates distinct evolutionary pathways within I. labrosus in the Tibagi River basin. ©FUNPEC-RP.
- Published
- 2008
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33. Karyotypic Studies in the Characidae Fish, Genus Astyanax. An Endemic and Highly Isolated Population of Astyanax sp
- Author
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Mara Cristina de Almeida, Roberto Ferreira Artoni, Marcelo Ricardo Vicari, Oscar Akio Shibatta, Luiz Antonio Carlos Bertollo, Orlando Moreira-Filho, and Daniele Aparecida Matoso
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Heterochromatin ,Zoology ,Karyotype ,Cell Biology ,Plant Science ,Biology ,biology.organism_classification ,Characidae ,Isolated population ,Genetics ,Constitutive heterochromatin ,Genus Astyanax ,Animal Science and Zoology ,Nucleolus organizer region ,Endemism - Abstract
The chromosomes of Astyanax sp. is now described for the first time. Ten specimens of a highly isolated population from the Sinkhole 1 (Vila Velha State Park, Ponta Grossa, PR, Brazil) were analyzed, with the characterization of the karyotype, constitutive heterochromatin and nucleolar organizer regions. Males and females of this species present a similar karyotypic structure (2n=48, 6M+18SM+14ST+10A), without morphologically differentiated sex chromosomes. A great variability concerning the heterochromatin located on the subtelo-acrocentric chromosomes appears also in this species, accordingly to that occur in some other Astyanax species.
- Published
- 2002
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34. Chromosomal polymorphism in two species of Hypancistrus (Siluriformes: Loricariidae): an integrative approach for understanding their biodiversity
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Daniele Aparecida Matoso, Emanuell D. Ribeiro, Tomas Hrbek, Leandro M. Sousa, Eliana Feldberg, Maelin da Silva, and Lúcia H. Rapp Py-Daniel
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Male ,Karyotype ,Plant Science ,DNA barcoding ,Chromosomes ,Evolution, Molecular ,Genetics ,Animals ,DNA Barcoding, Taxonomic ,Chromosomal polymorphism ,Catfishes ,Phylogeny ,Chromosomal inversion ,Synapomorphy ,biology ,Genetic Variation ,Chromosome ,Genes, rRNA ,General Medicine ,biology.organism_classification ,Diploidy ,Ancistrini ,Insect Science ,Chromosome Inversion ,Cytogenetic Analysis ,Female ,Animal Science and Zoology ,Ploidy - Abstract
Structural chromosome changes are widely described in different vertebrate groups and generate genetic, phenotypic and behavioral diversity. During the evolution of loricariids, several rearrangements (fissions, fusions, inversions) seem to have occurred. Hypancistrus, tribe Ancistrini, are highly demanded for fishkeeping around the world. In this tribe, the diploid chromosome number 2n = 52 is considered a synapomorphy, and paracentric-type inversions appear to be involved in the chromosomal evolution of the tribe. The present study investigated the karyotypes of H. zebra and H. cf. debilittera using cytogenetic, classical and molecular tools, as well as DNA barcoding. Data reveal that, although diploid number in both species corroborates the proposed synapomorphy for the tribe, there is a complex karyotype dynamics, reflected in the intense chromosomal polymorphism, resulting from rearrangements involving ribosomal regions (5S and 18S rDNA), which are suggested to be paracentric inversions. Besides, DNA barcode confirms reciprocal monophyletism between the species, validating the existence of two species, only. This scenario, coupled with genomic instability caused by exogenous sequences such as Rex-3 retrotransposons and the species' sedentary lifestyle, which helps the fast polymorphism fixation, may reflect different phenotypic color patterns in natural populations, as observed in H. cf. debilittera.
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- 2014
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35. Molecular taxonomy and evolutionary hypothesis concerning Astyanax fasciatus (Characiformes, Characidae) from Vila Velha State Park and Tibagi and Iguaçu Rivers
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Robledo de Almeida Torres, M. da Silva, R.F. Artoni, and Daniele Aparecida Matoso
- Subjects
Characiformes ,Molecular taxonomy ,Phylogeny ,Astyanax Altiparanae ,River ,biology ,Phylogenetic tree ,Geography ,General Medicine ,Ribosome Rna ,Classification ,Maximum parsimony ,Hemigrammus Bleheri ,Parsimony Analysis ,Chemistry ,Dna, Mitochondrial ,Brazil ,Dna Sequence ,Species complex ,Evolution ,Molecular Sequence Data ,Zoology ,Species Difference ,Rna, Ribosomal, 12s ,DNA, Mitochondrial ,Astyanax Fasciatus ,Molecular Genetics ,Evolution, Molecular ,Rivers ,Species Specificity ,Genetics ,Animals ,Rna, Ribosomal ,Molecular Biology ,Taxonomy ,Animal ,Characidae ,Brasil ,Nucleotide Sequence ,Bayes Theorem ,Sequence Analysis, Dna ,Sequence Analysis, DNA ,biology.organism_classification ,Astyanax fasciatus ,Nonhuman ,RNA, Ribosomal ,National Park ,Hemigrammus bleheri - Abstract
A species complex hypothesis involving Astyanax fasciatus from southern Brazil was tested using 12S mtDNA sequences. Phylogenetic inferences were performed with maximum likelihood, maximum parsimony and Bayesian as phylogenetic methods and Hemigrammus bleheri as the outgroup. Besides 11 sequences from A. fasciatus, the data set was comprised of other partial 12S sequences including material from Astyanax altiparanae (two sequences) and Astyanax sp (four sequences), both from the Iguaçu River. The hypothesis of an A. fasciatus species complex was reinforced given the close relationship between A. altiparanae and Astyanax sp observed in the Bayesian tree. Consequently, a taxonomic revision is necessary for these species. © FUNPEC-RP.
- Published
- 2013
36. Genetic Diversity of the Small Characid Fish Astyanax sp., and its Significance for Conservation
- Author
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Pedro M. Galetti, Roberto Ferreira Artoni, and Daniele Aparecida Matoso
- Subjects
Conservation genetics ,Genetic diversity ,Similarity (network science) ,Ecology ,Genetic variation ,Biodiversity ,Genetic variability ,Aquatic Science ,Biology ,RAPD ,Hydrobiology - Abstract
Genetic variations in Astyanax sp. fish from Vila Velha State Park and Tibagi river (Parana, Brazil) were analyzed by RAPD. Intrapopulational genetic similarity was analyzed at three collection sites. The higher RAPD polymorphism observed at site 3 (Tibagi river) suggests either an overlapping of structured populations or the presence of coexisting morphotypes at this site. Significant p values obtained by homogeneity tests revealed an unequal loci proportion among populations. The significance of similarity coefficients indicated that the differences observed among populations are relevant. These findings provide important information for the management and conservation of the species analyzed and for genetic variability maintenance programs.
- Published
- 2004
- Full Text
- View/download PDF
37. Epifluorescence and light microscopy evidencing structural and functional polymorphism of ribosomal DNA in fish (Teleostei: Astyanax fasciatus)
- Author
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Roberto Ferreira Artoni, Carlos Henrique Schneider, Marcelo Ricardo Vicari, Mara Cristina de Almeida, Daniele Aparecida Matoso, and Maria Claudia Gross
- Subjects
Genetics ,Male ,Species complex ,Teleostei ,Polymorphism, Genetic ,biology ,Fishes ,General Physics and Astronomy ,Chromosome ,Cell Biology ,Ribosomal RNA ,biology.organism_classification ,Physical Chromosome Mapping ,DNA, Ribosomal ,Chromosome 4 ,Microscopy, Fluorescence ,Structural Biology ,Nucleolus Organizer Region ,Animals ,General Materials Science ,Female ,Ploidy ,Ribosomal DNA ,Gene ,In Situ Hybridization, Fluorescence - Abstract
New karyotypic data are presented for the Astyanax fasciatus species complex from four different locations on the Upper Tibagi River in the state of Parana, Brazil. Chromosome markers were analyzed using conventional (Ag-NOR) and molecular (FISH with 18S biotinylated probes) methods. Two cytotypes were found in cell counts with diploid number 2n = 48 chromosomes and 2n = 50 chromosomes, previously denominated Cytotype A and B, respectively. Two specific patterns of Ag-NORs markers (ribosomal gene activity) were found, with intra-population and inter-population variations. Cytotype A exhibited two to three chromosomes with NOR sites in the metaphases analyzed. In Cytotype B specimens, up to three markers were found, demonstrating greater intra-population and inter-population variation. All individuals with only one chromosome pair with NORs were located in the telomeric region of the short arm of Chromosome 5. This characteristic was interpreted as ancestral for the species. Another identified pattern revealed a site in the telomeric region probably in the long arm of Chromosome 4 and another submetacentric chromosome with bitelomeric marks exclusively in specimens with 2n = 50 chromosomes. In the FISH analysis (ribosomal gene structure), five to seven markers were identified in Cytotype A and three to seven markers were identified in Cytotype B. Structural chromosome events and/or transposable elements are required to explain the ribosomal gene location diversity in these organisms. The results of the present study corroborate the hypothesis that the A. fasciatus of the Upper Tibagi River region constitute a species complex.
- Published
- 2008
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