1. Spatial-Temporal Co-Circulation of Dengue Virus 1, 2, 3, and 4 Associated with Coinfection Cases in a Hyperendemic Area of Brazil: A 4-Week Survey
- Author
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Paulo César Peregrino Ferreira, Hassan M. Zibaoui, Jônatas Santos Abrahão, Ana Paula Pessoa Vilela, Júlio César Câmara Rosa, Valdelaine Etelvina Miranda de Araújo, Daniela P J Miranda, Jaquelline Germano de Oliveira, Elisa H.P. Andrade, Erna Geessien Kroon, and Leandra Barcelos Figueiredo
- Subjects
0301 basic medicine ,Serotype ,Time Factors ,Endemic Diseases ,viruses ,Dengue virus ,medicine.disease_cause ,law.invention ,Dengue fever ,Dengue ,03 medical and health sciences ,law ,Virology ,Genotype ,Medicine ,Humans ,Polymerase chain reaction ,business.industry ,Coinfection ,Outbreak ,virus diseases ,Articles ,biochemical phenomena, metabolism, and nutrition ,Dengue Virus ,medicine.disease ,Reverse transcriptase ,030104 developmental biology ,Infectious Diseases ,Parasitology ,business ,Brazil - Abstract
Dengue is currently regarded as a major public health problem worldwide. In a hyperendemic region during an outbreak, we detected the co-circulation of all Dengue virus (DENV) serotypes including two different genotypes of DENV-3 and DENV-4, and concurrent infections with up to three serotypes were identified in symptomatic patients. A total of 49 acute phase plasma samples from patients clinically suspected of dengue were collected during the 4 weeks of May 2013. DENV-1-4 was detected by reverse transcriptase semi-nested polymerase chain reaction in 33 samples (67.3%), of which 26 DNA fragments were sequenced. Twenty samples (76.9%) were identified with a single DENV serotype and six (23.1%) with more than one serotype. DENV-3 was the predominant serotype of the outbreak. On the basis of phylogenetic analyses, DENV-1 isolates belong to genotype V, DENV-2 to American-Asian genotype, DENV-3 to genotypes I and III, and DENV-4 to genotypes I and II.
- Published
- 2015