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3. Inhibition of GPR68 kills glioblastoma in zebrafish xenograft models

Author

Leif R. Neitzel, Daniela T. Fuller, Charles H. Williams, and Charles C. Hong

Subjects
Glioblastoma multiforme, Ogremorphin, Zebrafish, Xenograft, GPR68, OGR1, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390
Abstract

Abstract Objective Inhibition and knockdown of GPR68 negatively affects glioblastoma cell survival in vitro by inducing ferroptosis. Herein, we aimed to demonstrate that inhibition of GPR68 reduces the survival of glioblastoma cells in vivo using two orthotopic larval xenograft models in Danio rerio, using GBM cell lines U87-MG and U138-MG. In vivo survival of the cancer cells was assessed in the setting of GPR68 inhibition or knockdown. Results In vitro, shRNA-mediated knockdown of GPR68 inhibition demonstrated potent cytotoxic effects against U87 and U138 glioblastoma cell lines. This effect was associated with increased intracellular lipid peroxidation, suggesting ferroptosis as the underlying mechanism of cell death. Translating these findings in vivo, we established a novel xenograft model in zebrafish by successfully grafting fluorescently labeled human glioblastoma cells, which were previously shown to overexpress GPR68. shRNA knockdown of GPR68 significantly reduced the viability of grafted GBM cells within this model. Additionally, treatment with ogremorphin (OGM), a highly specific small molecule inhibitor of GPR68, also reduced the viability of grafted GBM cells with limited toxicity to the developing zebrafish embryos. This study suggests that therapeutic targeting of GPR68 with small molecules like OGM represents a promising approach for the treatment of GBM.

Published
2024
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4. EP-261 - ESTIMATIVA DE PREVALÊNCIA E TENDÊNCIA TEMPORAL DE PERFIS SUGESTIVOS DE STAPHYLOCOCCUS AUREUS RESISTENTE A METICILINA DA COMUNIDADE (CA-MRSA) EM SÃO PAULO DE 2011 A 2019

Author

Aline Santos Ibanes, Thaís F.T. Resende, Ana Silvia S. Marinonio, Jussimara M. Nurmberger, Fernanda M. Inoue, Thaina A.D. Passos, Daniela T. Costa-Nobre, Sergio Tufik, and Carlos Kiffer

Subjects
Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502
Abstract

Introdução: A prevalência de infecções causadas por CA-MRSA tem aumentado globalmente. No Brasil, sua epidemiologia ainda é pouco conhecida. Estudos genotípicos já demonstraram correlação entre perfis de sensibilidade e tipos de cassetes cromossômicos associados a CA-MRSA (SCCmec tipos IV ou V). Perfis fenotípicos podem auxiliar como marcadores sugestivos de CA-MRSA em estudos epidemiológicos. Objetivo: Estimar a prevalência e a tendência temporal de perfis sugestivos de CA-MRSA no Estado de São Paulo, Brasil, entre 2011 e 2019. Método: Análise retrospectiva em base de dados de laboratório clínico entre 2011 e 2019, com isolados em cultura de Staphylococcus aureus (SA) únicos por paciente, identificados em amostras clínicas de rotina e com antibiograma (método conforme rotina e padronizado por BrCAST do respectivo ano). A sensibilidade a clindamicina (CLI), ciprofloxacino (CIP) e Sulfametoxazol/trimetroprim (TMP-SMX) com resistência concomitante a oxacilina (OXA) foram usados como perfis sugestivos de CA-MRSA. As taxas de OXA-R com CLI-S ou CIP-S ou TMP-SMX-S foram calculadas utilizando o número total de isolados para cada antibiotico por ano. A tendência temporal foi estimada utilizando-se o modelo Prais-Winsten, expresso por annual percent change (APC) com intervalo de confiança (95% CI). Resultados: No total, 50,858 isolados de SA foram identificados entre 2011 e 2019 (22,346 (42.8%) hemoculturas, 6,820 (13.1%) uroculturas e 23,058 (44.1%) de outros materiais). Destes 21,677 eram OXA-R (42.6%), com 4,442 (20.5%) CLI-S, 4,230 (19.7%) CIP-S, e 21,484 (99.1%) TMP-SMX-S. No período, houve tendência de aumento para os perfis OXA-R + CLI-S (APC 6.92; 95% CI: 0.91 a 13.29), estacionária para OXA-R + CIP-S (APC -6.35; 95% CI: -20.14 a 9.83), e de redução para OXA-R + TMP-SMX-S (APC -2.63; 95% CI: -3.53 a -1.71). Conclusão: O perfil OXA-R + CLI-S já foi correlacionado a perfil genotípico sugestivo de CA-MRSA. Este perfil representou 20.5% do total de MRSA e 8.7% do total de SA detectados no estado, tendo havido aumento temporal de detecção deste fenótipo ao longo dos anos. Marcadores fenotípicos sugestivos de perfis epidemiológicos podem ser úteis em estudos com grandes bases de dados, potencialmente otimizando recursos e o uso de marcadores genéticos ou moleculares.

Published
2024
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5. Monocyte to high-density lipoprotein cholesterol ratio is correlated with baseline anthropometric measurements in patients with obesity but not with weight-loss process after sleeve gastrectomy - An observational cohort study

Author

Sala Daniela T., Kiss Botond I., Daniealopol Ruxandra, Fodor Stefania R., Daniealopol Valentin, Soo Arpad, Calin Constantin, Muresan Mircea G., and Neagoe Radu M.

Subjects
weight loss, body measures, partial gastrectomy, high-density lipoprotein cholesterol, monocyte, Medicine
Abstract

Introductionː A strong connection between inflammation and obesity was repeatedly described, with the latter defined as a chronic low-degree systemic inflammatory state. This study analyzed the correlations between inflammatory blood indexes and both baseline anthropometric measurements and the weight–loss process after bariatric surgery.

Published
2024
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6. Human liver microbiota modeling strategy at the early onset of fibrosis

Author

Champion, Camille, Neagoe, Radu M., Effernberger, Maria, Sala, Daniela T., Servant, Florence, Christensen, Jeffrey E., Arnoriaga-Rodriguez, Maria, Amar, Jacques, Lelouvier, Benjamin, Loubieres, Pascale, Azalbert, Vincent, Minty, Matthieu, Thomas, Charlotte, Blasco-Baque, Vincent, Gamboa, Fabrice, Tilg, Herbert, Cardellini, Marina, Federici, Massimo, Fernández-Real, Jose-Manuel, Loubes, Jean Michel, and Burcelin, Rémy

Published
2023
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9. 2023 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations: Summary From the Basic Life Support; Advanced Life Support; Pediatric Life Support; Neonatal Life Support; Education, Implementation, and Teams; and First Aid Task Forces

Author

Barcala-Furelos, Roberto, Beerman, Stephen B., Bruckner, Marlies, Castrén, Maaret, Chong, ShuLing, Claesson, Andreas, Dunne, Cody L., Finan, Emer, Fukuda, Tatsuma, Lalgudi Ganesan, Saptharishi, Gately, Callum, Gois, Aecio, Gray, Seth, Halamek, Louis P., Hoover, Amber V., Hurst, Cameron, Josephsen, Justin, Kollander, Louise, Omar Kamlin, C., Kool, Mirjam, Li, Lei, Mecrow, Thomas S., Montgomery, William, Ristau, Patrick, Jayashree, Muralidharan, Schmidt, Andrew, Scquizzato, Tommaso, Seesink, Jeroen, Sempsrott, Justin, Lee Solevåg, Anne, Strand, Marya L., Szpilman, David, Szyld, Edgardo, Thom, Ogilvie, Tobin, Joshua M., Trang, Jacinta, Webber, Jonathon, Webster, Hannah K., Wellsford, Michelle, Berg, Katherine M., Bray, Janet E., Ng, Kee-Chong, Liley, Helen G., Greif, Robert, Carlson, Jestin N., Morley, Peter T., Drennan, Ian R., Smyth, Michael, Scholefield, Barnaby R., Weiner, Gary M., Cheng, Adam, Djärv, Therese, Abelairas-Gómez, Cristian, Acworth, Jason, Andersen, Lars W., Atkins, Dianne L., Berry, David C., Bhanji, Farhan, Bierens, Joost, Bittencourt Couto, Thomaz, Borra, Vere, Böttiger, Bernd W., Bradley, Richard N., Breckwoldt, Jan, Cassan, Pascal, Chang, Wei-Tien, Charlton, Nathan P., Chung, Sung Phil, Considine, Julie, Costa-Nobre, Daniela T., Couper, Keith, Dainty, Katie N., Dassanayake, Vihara, Davis, Peter G., Dawson, Jennifer A., Fernanda de Almeida, Maria, De Caen, Allan R., Deakin, Charles D., Dicker, Bridget, Douma, Matthew J., Eastwood, Kathryn, El-Naggar, Walid, Fabres, Jorge G., Fawke, Joe, Fijacko, Nino, Finn, Judith C., Flores, Gustavo E., Foglia, Elizabeth E., Folke, Fredrik, Gilfoyle, Elaine, Goolsby, Craig A., Granfeldt, Asger, Guerguerian, Anne-Marie, Guinsburg, Ruth, Hatanaka, Tetsuo, Hirsch, Karen G., Holmberg, Mathias J., Hosono, Shigeharu, Hsieh, Ming-Ju, Hsu, Cindy H., Ikeyama, Takanari, Isayama, Tetsuya, Johnson, Nicholas J., Kapadia, Vishal S., Daripa Kawakami, Mandira, Kim, Han-Suk, Kleinman, Monica E., Kloeck, David A., Kudenchuk, Peter, Kule, Amy, Kurosawa, Hiroshi, Lagina, Anthony T., Lauridsen, Kasper G., Lavonas, Eric J., Lee, Henry C., Lin, Yiqun, Lockey, Andrew S., Macneil, Finlay, Maconochie, Ian K., John Madar, R., Malta Hansen, Carolina, Masterson, Siobhan, Matsuyama, Tasuku, McKinlay, Christopher J.D., Meyran, Daniel, Monnelly, Vix, Nadkarni, Vinay, Nakwa, Firdose L., Nation, Kevin J., Nehme, Ziad, Nemeth, Michael, Neumar, Robert W., Nicholson, Tonia, Nikolaou, Nikolaos, Nishiyama, Chika, Norii, Tatsuya, Nuthall, Gabrielle A., Ohshimo, Shinchiro, Olasveengen, Theresa M., Gene Ong, Yong-Kwang, Orkin, Aaron M., Parr, Michael J., Patocka, Catherine, Perkins, Gavin D., Perlman, Jeffrey M., Rabi, Yacov, Raitt, James, Ramachandran, Shalini, Ramaswamy, Viraraghavan V., Raymond, Tia T., Reis, Amelia G., Reynolds, Joshua C., Ristagno, Giuseppe, Rodriguez-Nunez, Antonio, Roehr, Charles C., Rüdiger, Mario, Sakamoto, Tetsuya, Sandroni, Claudio, Sawyer, Taylor L., Schexnayder, Steve M., Schmölzer, Georg M., Schnaubelt, Sebastian, Semeraro, Federico, Singletary, Eunice M., Skrifvars, Markus B., Smith, Christopher M., Soar, Jasmeet, Stassen, Willem, Sugiura, Takahiro, Tijssen, Janice A., Topjian, Alexis A., Trevisanuto, Daniele, Vaillancourt, Christian, Wyckoff, Myra H., Wyllie, Jonathan P., Yang, Chih-Wei, Yeung, Joyce, Zelop, Carolyn M., Zideman, David A., and Nolan, Jerry P.

Published
2024
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10. 2024 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations: Summary From the Basic Life Support; Advanced Life Support; Pediatric Life Support; Neonatal Life Support; Education, Implementation, and Teams; and First Aid Task Forces

Author

Greif, Robert, Bray, Janet E., Djärv, Therese, Drennan, Ian R., Liley, Helen G., Ng, Kee-Chong, Cheng, Adam, Douma, Matthew J., Scholefield, Barnaby R., Smyth, Michael, Weiner, Gary, Abelairas-Gómez, Cristian, Acworth, Jason, Anderson, Natalie, Atkins, Dianne L., Berry, David C., Bhanji, Farhan, Böttiger, Bernd W., Bradley, Richard N., Breckwoldt, Jan, Carlson, Jestin N., Cassan, Pascal, Chang, Wei-Tien, Charlton, Nathan P., Phil Chung, Sung, Considine, Julie, Cortegiani, Andrea, Costa-Nobre, Daniela T., Couper, Keith, Bittencourt Couto, Thomaz, Dainty, Katie N., Dassanayake, Vihara, Davis, Peter G., Dawson, Jennifer A., de Caen, Allan R., Deakin, Charles D., Debaty, Guillaume, del Castillo, Jimena, Dewan, Maya, Dicker, Bridget, Djakow, Jana, Donoghue, Aaron J., Eastwood, Kathryn, El-Naggar, Walid, Escalante-Kanashiro, Raffo, Fabres, Jorge, Farquharson, Barbara, Fawke, Joe, Fernanda de Almeida, Maria, Fernando, Shannon M., Finan, Emer, Finn, Judith, Flores, Gustavo E., Foglia, Elizabeth E., Folke, Fredrik, Goolsby, Craig A., Granfeldt, Asger, Guerguerian, Anne-Marie, Guinsburg, Ruth, Malta Hansen, Carolina, Hatanaka, Tetsuo, Hirsch, Karen G., Holmberg, Mathias J., Hooper, Stuart, Hoover, Amber V., Hsieh, Ming-Ju, Ikeyama, Takanari, Isayama, Tetsuya, Johnson, Nicholas J., Josephsen, Justin, Katheria, Anup, Kawakami, Mandira D., Kleinman, Monica, Kloeck, David, Ko, Ying-Chih, Kudenchuk, Peter, Kule, Amy, Kurosawa, Hiroshi, Laermans, Jorien, Lagina, Anthony, Lauridsen, Kasper G., Lavonas, Eric J., Lee, Henry C., Han Lim, Swee, Lin, Yiqun, Lockey, Andrew S., Lopez-Herce, Jesus, Lukas, George, Macneil, Finlay, Maconochie, Ian K., Madar, John, Martinez-Mejas, Abel, Masterson, Siobhan, Matsuyama, Tasuku, Mausling, Richard, McKinlay, Christopher J.D., Meyran, Daniel, Montgomery, William, Morley, Peter T., Morrison, Laurie J., Moskowitz, Ari L., Myburgh, Michelle, Nabecker, Sabine, Nadkarni, Vinay, Nakwa, Firdose, Nation, Kevin J., Nehme, Ziad, Nicholson, Tonia, Nikolaou, Nikolaos, Nishiyama, Chika, Norii, Tatsuya, Nuthall, Gabrielle, Ohshimo, Shinichiro, Olasveengen, Theresa, Olaussen, Alexander, Ong, Gene, Orkin, Aaron, Parr, Michael J., Perkins, Gavin D., Pocock, Helen, Rabi, Yacov, Raffay, Violetta, Raitt, James, Raymond, Tia, Ristagno, Giuseppe, Rodriguez-Nunez, Antonio, Rossano, Joseph, Rüdiger, Mario, Sandroni, Claudio, Sawyer, Taylor L., Schexnayder, Stephen M., Schmölzer, Georg, Schnaubelt, Sebastian, Lene Seidler, Anna, Semeraro, Federico, Singletary, Eunice M., Skrifvars, Markus B., Smith, Christopher M., Soar, Jasmeet, Lee Solevåg, Anne, Soll, Roger, Stassen, Willem, Sugiura, Takahiro, Thilakasiri, Kaushila, Tijssen, Janice, Kumar Tiwari, Lokesh, Topjian, Alexis, Trevisanuto, Daniele, Vaillancourt, Christian, Welsford, Michelle, Wyckoff, Myra H., Yang, Chih-Wei, Yeung, Joyce, Zelop, Carolyn M., Zideman, David A., Nolan, Jerry P., and Berg, Katherine M.

Published
2024
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11. 2023 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations: Summary From the Basic Life Support; Advanced Life Support; Pediatric Life Support; Neonatal Life Support; Education, Implementation, and Teams; and First Aid Task Forces

Author

Berg, Katherine M., Bray, Janet E., Ng, Kee-Chong, Liley, Helen G., Greif, Robert, Carlson, Jestin N., Morley, Peter T., Drennan, Ian R., Smyth, Michael, Scholefield, Barnaby R., Weiner, Gary M., Cheng, Adam, Djärv, Therese, Abelairas-Gómez, Cristian, Acworth, Jason, Andersen, Lars W., Atkins, Dianne L., Berry, David C., Bhanji, Farhan, Bierens, Joost, Bittencourt Couto, Thomaz, Borra, Vere, Böttiger, Bernd W., Bradley, Richard N., Breckwoldt, Jan, Cassan, Pascal, Chang, Wei-Tien, Charlton, Nathan P., Chung, Sung Phil, Considine, Julie, Costa-Nobre, Daniela T., Couper, Keith, Dainty, Katie N., Dassanayake, Vihara, Davis, Peter G., Dawson, Jennifer A., de Almeida, Maria Fernanda, De Caen, Allan R., Deakin, Charles D., Dicker, Bridget, Douma, Matthew J., Eastwood, Kathryn, El-Naggar, Walid, Fabres, Jorge G., Fawke, Joe, Fijacko, Nino, Finn, Judith C., Flores, Gustavo E., Foglia, Elizabeth E., Folke, Fredrik, Gilfoyle, Elaine, Goolsby, Craig A., Granfeldt, Asger, Guerguerian, Anne-Marie, Guinsburg, Ruth, Hatanaka, Tetsuo, Hirsch, Karen G., Holmberg, Mathias J., Hosono, Shigeharu, Hsieh, Ming-Ju, Hsu, Cindy H., Ikeyama, Takanari, Isayama, Tetsuya, Johnson, Nicholas J., Kapadia, Vishal S., Kawakami, Mandira Daripa, Kim, Han-Suk, Kleinman, Monica E., Kloeck, David A., Ko, Ying-Chih, Kudenchuk, Peter, Kule, Amy, Kurosawa, Hiroshi, Lagina, Anthony T., Lauridsen, Kasper G., Lavonas, Eric J., Lee, Henry C., Lin, Yiqun, Lockey, Andrew S., Macneil, Finlay, Maconochie, Ian K., Madar, R. John, Malta Hansen, Carolina, Masterson, Siobhan, Matsuyama, Tasuku, McKinlay, Christopher J.D., Meyran, Daniel, Monnelly, Vix, Morrison, Laurie J., Nadkarni, Vinay, Nakwa, Firdose L., Nation, Kevin J., Nehme, Ziad, Nemeth, Michael, Neumar, Robert W., Nicholson, Tonia, Nikolaou, Nikolaos, Nishiyama, Chika, Norii, Tatsuya, Nuthall, Gabrielle A., Ohshimo, Shinchiro, Olasveengen, Theresa M., Ong, Yong-Kwang Gene, Orkin, Aaron M., Parr, Michael J., Patocka, Catherine, Perkins, Gavin D., Perlman, Jeffrey M., Rabi, Yacov, Raitt, James, Ramachandran, Shalini, Ramaswamy, Viraraghavan V., Raymond, Tia T., Reis, Amelia G., Reynolds, Joshua C., Ristagno, Giuseppe, Rodriguez-Nunez, Antonio, Roehr, Charles C., Rüdiger, Mario, Sakamoto, Tetsuya, Sandroni, Claudio, Sawyer, Taylor L., Schexnayder, Steve M., Schmölzer, Georg M., Schnaubelt, Sebastian, Semeraro, Federico, Singletary, Eunice M., Skrifvars, Markus B., Smith, Christopher M., Soar, Jasmeet, Stassen, Willem, Sugiura, Takahiro, Tijssen, Janice A., Topjian, Alexis A., Trevisanuto, Daniele, Vaillancourt, Christian, Wyckoff, Myra H., Wyllie, Jonathan P., Yang, Chih-Wei, Yeung, Joyce, Zelop, Carolyn M., Zideman, David A., Nolan, Jerry P., Barcala-Furelos, Roberto, Beerman, Stephen B., Castrén, Maaret, Chong, ShuLing, Claesson, Andreas, Dunne, Cody L., Ersdal, Hege L., Finan, Emer, Fuerch, Janene, Fukuda, Tatsuma, Ganesan, Saptharishi Lalgudi, Gately, Callum, Gray, Seth, Halamek, Louis P., Hoover, Amber V., Kollander, Louise, Kamlin, C. Omar, Koo, Mirjam, Li, Lei, Leone, Tina A., Mecrow, s, Montgomery, William, Ristau, Patrick, Jayashree, Muralidharan, Quek, Bin Huey, Schmidt, Andrew, Scquizzato, Tommaso, Seesink, Jeroen, Sempsrott, Justin, Shah, Birju A., Strand, Marya L., Szpilman, David, Szyld, Edgardo, Thio, Marta, Thom, Ogilvie, Tobin, Joshua M., Udaeta, Enrique, Webber, Jonathon, Webster, Hannah K., Wellsford, Michelle, and Yamada, Nicole K.

Published
2023
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13. Linkage-specific deubiquitylation by OTUD5 defines an embryonic pathway intolerant to genomic variation

Author

Beck, David B, Basar, Mohammed A, Asmar, Anthony J, Thompson, Joyce J, Oda, Hirotsugu, Uehara, Daniela T, Saida, Ken, Pajusalu, Sander, Talvik, Inga, D’Souza, Precilla, Bodurtha, Joann, Mu, Weiyi, Barañano, Kristin W, Miyake, Noriko, Wang, Raymond, Kempers, Marlies, Tamada, Tomoko, Nishimura, Yutaka, Okada, Satoshi, Kosho, Tomoki, Dale, Ryan, Mitra, Apratim, Macnamara, Ellen, Matsumoto, Naomichi, Inazawa, Johji, Walkiewicz, Magdalena, Õunap, Katrin, Tifft, Cynthia J, Aksentijevich, Ivona, Kastner, Daniel L, Rocha, Pedro P, and Werner, Achim

Subjects
Biochemistry and Cell Biology, Biomedical and Clinical Sciences, Genetics, Biological Sciences, Human Genome, Rare Diseases, Pediatric, Generic health relevance, Chromatin, Genomics, Humans, Signal Transduction, Ubiquitin, Ubiquitination, Undiagnosed Diseases Network
Abstract

Reversible modification of proteins with linkage-specific ubiquitin chains is critical for intracellular signaling. Information on physiological roles and underlying mechanisms of particular ubiquitin linkages during human development are limited. Here, relying on genomic constraint scores, we identify 10 patients with multiple congenital anomalies caused by hemizygous variants in OTUD5, encoding a K48/K63 linkage-specific deubiquitylase. By studying these mutations, we find that OTUD5 controls neuroectodermal differentiation through cleaving K48-linked ubiquitin chains to counteract degradation of select chromatin regulators (e.g., ARID1A/B, histone deacetylase 2, and HCF1), mutations of which underlie diseases that exhibit phenotypic overlap with OTUD5 patients. Loss of OTUD5 during differentiation leads to less accessible chromatin at neuroectodermal enhancers and aberrant gene expression. Our study describes a previously unidentified disorder we name LINKED (LINKage-specific deubiquitylation deficiency-induced Embryonic Defects) syndrome and reveals linkage-specific ubiquitin cleavage from chromatin remodelers as an essential signaling mode that coordinates chromatin remodeling during embryogenesis.

Published
2021

15. Human liver microbiota modeling strategy at the early onset of fibrosis

Author

Camille Champion, Radu M. Neagoe, Maria Effernberger, Daniela T. Sala, Florence Servant, Jeffrey E. Christensen, Maria Arnoriaga-Rodriguez, Jacques Amar, Benjamin Lelouvier, Pascale Loubieres, Vincent Azalbert, Matthieu Minty, Charlotte Thomas, Vincent Blasco-Baque, Fabrice Gamboa, Herbert Tilg, Marina Cardellini, Massimo Federici, Jose-Manuel Fernández-Real, Jean Michel Loubes, and Rémy Burcelin

Subjects
Biomathematics, Liver diseases, Metabolic disease, Microbiota, Tissue microbiota, Microbiology, QR1-502
Abstract

Abstract Background Gut microbiota is involved in the development of liver diseases such as fibrosis. We and others identified that selected sets of gut bacterial DNA and bacteria translocate to tissues, notably the liver, to establish a non-infectious tissue microbiota composed of microbial DNA and a low frequency live bacteria. However, the precise set of bacterial DNA, and thereby the corresponding taxa associated with the early stages of fibrosis need to be identified. Furthermore, to overcome the impact of different group size and patient origins we adapted innovative statistical approaches. Liver samples with low liver fibrosis scores (F0, F1, F2), to study the early stages of the disease, were collected from Romania(n = 36), Austria(n = 10), Italy(n = 19), and Spain(n = 17). The 16S rRNA gene was sequenced. We considered the frequency, sparsity, unbalanced sample size between cohorts to identify taxonomic profiles and statistical differences. Results Multivariate analyses, including adapted spectral clustering with L1-penalty fair-discriminant strategies, and predicted metagenomics were used to identify that 50% of liver taxa associated with the early stage fibrosis were Enterobacteriaceae, Pseudomonadaceae, Xanthobacteriaceae and Burkholderiaceae. The Flavobacteriaceae and Xanthobacteriaceae discriminated between F0 and F1. Predicted metagenomics analysis identified that the preQ0 biosynthesis and the potential pathways involving glucoryranose and glycogen degradation were negatively associated with liver fibrosis F1-F2 vs F0. Conclusions Without demonstrating causality, our results suggest first a role of bacterial translocation to the liver in the progression of fibrosis, notably at the earliest stages. Second, our statistical approach can identify microbial signatures and overcome issues regarding sample size differences, the impact of environment, and sets of analyses. Trial registration TirguMECCH ROLIVER Prospective Cohort for the Identification of Liver Microbiota, registration 4065/2014. Registered 01 01 2014.

Published
2023
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16. Corrigendum to “2022 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations: Summary From the Basic Life Support; Advanced Life Support; Pediatric Life Support; Neonatal Life Support; Education, Implementation, and Teams; and First Aid Task Forces” [Resuscitation 181 (2022) 208–288]

Author

Wyckoff, Myra H., primary, Greif, Robert, additional, Morley, Peter T., additional, Ng, Kee-Chong, additional, Olasveengen, Theresa M., additional, Singletary, Eunice M., additional, Soar, Jasmeet, additional, Cheng, Adam, additional, Drennan, Ian R., additional, Liley, Helen G., additional, Scholefield, Barnaby R., additional, Smyth, Michael A., additional, Welsford, Michelle, additional, Zideman, David A., additional, Acworth, Jason, additional, Aickin, Richard, additional, Andersen, Lars W., additional, Atkins, Diane, additional, Berry, David C., additional, Bhanji, Farhan, additional, Bierens, Joost, additional, Borra, Vere, additional, Böttiger, Bernd W., additional, Bradley, Richard N., additional, Bray, Janet E., additional, Breckwoldt, Jan, additional, Callaway, Clifton W., additional, Carlson, Jestin N., additional, Cassan, Pascal, additional, Castrén, Maaret, additional, Chang, Wei-Tien, additional, Charlton, Nathan P., additional, Chung, Sung Phil, additional, Considine, Julie, additional, Costa-Nobre, Daniela T., additional, Couper, Keith, additional, Couto, Thomaz Bittencourt, additional, Dainty, Katie N., additional, Davis, Peter G., additional, de Almeida, Maria Fernanda, additional, de Caen, Allan R., additional, Deakin, Charles D., additional, Djärv, Therese, additional, Donnino, Michael W., additional, Douma, Matthew J., additional, Duff, Jonathan P., additional, Dunne, Cody L., additional, Eastwood, Kathryn, additional, El-Naggar, Walid, additional, Fabres, Jorge G., additional, Fawke, Joe, additional, Finn, Judith, additional, Foglia, Elizabeth E., additional, Folke, Fredrik, additional, Gilfoyle, Elaine, additional, Goolsby, Craig A., additional, Granfeldt, Asger, additional, Guerguerian, Anne-Marie, additional, Guinsburg, Ruth, additional, Hirsch, Karen G., additional, Holmberg, Mathias J., additional, Hosono, Shigeharu, additional, Hsieh, Ming-Ju, additional, Hsu, Cindy H., additional, Ikeyama, Takanari, additional, Isayama, Tetsuya, additional, Johnson, Nicholas J., additional, Kapadia, Vishal S., additional, Daripa Kawakami, Mandira, additional, Kim, Han-Suk, additional, Kleinman, Monica, additional, Kloeck, David A., additional, Kudenchuk, Peter J., additional, Lagina, Anthony T., additional, Lauridsen, Kasper G., additional, Lavonas, Eric J., additional, Lee, Henry C., additional, (Jeffrey) Lin, Yiqun, additional, Lockey, Andrew S., additional, Maconochie, Ian K., additional, John Madar, R., additional, Hansen, Carolina Malta, additional, Masterson, Siobhan, additional, Matsuyama, Tasuku, additional, McKinlay, Christopher J.D., additional, Meyran, Daniel, additional, Morgan, Patrick, additional, Morrison, Laurie J., additional, Nadkarni, Vinay, additional, Nakwa, Firdose L., additional, Nation, Kevin J., additional, Nehme, Ziad, additional, Nemeth, Michael, additional, Neumar, Robert W., additional, Nicholson, Tonia, additional, Nikolaou, Nikolaos, additional, Nishiyama, Chika, additional, Norii, Tatsuya, additional, Nuthall, Gabrielle A., additional, O'Neill, Brian J., additional, Gene Ong, Yong-Kwang, additional, Orkin, Aaron M., additional, Paiva, Edison F., additional, Parr, Michael J., additional, Patocka, Catherine, additional, Pellegrino, Jeffrey L., additional, Perkins, Gavin D., additional, Perlman, Jeffrey M., additional, Rabi, Yacov, additional, Reis, Amelia G., additional, Reynolds, Joshua C., additional, Ristagno, Giuseppe, additional, Rodriguez-Nunez, Antonio, additional, Roehr, Charles C., additional, Rüdiger, Mario, additional, Sakamoto, Tetsuya, additional, Sandroni, Claudio, additional, Sawyer, Taylor L., additional, Schexnayder, Steve M., additional, Schmölzer, Georg M., additional, Schnaubelt, Sebastian, additional, Semeraro, Federico, additional, Skrifvars, Markus B., additional, Smith, Christopher M., additional, Sugiura, Takahiro, additional, Tijssen, Janice A., additional, Trevisanuto, Daniele, additional, Van de Voorde, Patrick, additional, Wang, Tzong-Luen, additional, Weiner, Gary M., additional, Wyllie, Jonathan P., additional, Yang, Chih-Wei, additional, Yeung, Joyce, additional, Nolan, Jerry P., additional, Berg, Katherine M., additional, Burdick, Madeline C., additional, Cartledge, Susie, additional, Dawson, Jennifer A., additional, Elgohary, Moustafa M., additional, Ersdal, Hege L., additional, Finan, Emer, additional, Flaatten, Hilde I., additional, Flores, Gustavo E., additional, Fuerch, Janene, additional, Garg, Rakesh, additional, Gately, Callum, additional, Goh, Mark, additional, Halamek, Louis P., additional, Handley, Anthony J., additional, Hatanaka, Tetsuo, additional, Hoover, Amber, additional, Issa, Mohmoud, additional, Johnson, Samantha, additional, Kamlin, C. Omar, additional, Ko, Ying-Chih, additional, Kule, Amy, additional, Leone, Tina A., additional, MacKenzie, Ella, additional, Macneil, Finlay, additional, Montgomery, William, additional, O'Dochartaigh, Domhnall, additional, Ohshimo, Shinichiro, additional, Palazzo, Francesco Stefano, additional, Picard, Christopher, additional, Quek, Bin Huey, additional, Raitt, James, additional, Ramaswamy, Viraraghavan V., additional, Scapigliati, Andrea, additional, Shah, Birju A., additional, Stewart, Craig, additional, Strand, Marya L., additional, Szyld, Edgardo, additional, Thio, Marta, additional, Topjian, Alexis A., additional, Udaeta, Enrique, additional, Vaillancourt, Christian, additional, Wetsch, Wolfgang A., additional, Wigginton, Jane, additional, Yamada, Nicole K., additional, Yao, Sarah, additional, Zace, Drieda, additional, and Zelop, Carolyn M., additional

Published
2024
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17. 2022 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations: Summary From the Basic Life Support; Advanced Life Support; Pediatric Life Support; Neonatal Life Support; Education, Implementation, and Teams; and First Aid Task Forces

Author

Burdick, Madeline C., Cartledge, Susie, Dawson, Jennifer A., Elgohary, Moustafa M., Ersdal, Hege L., Finan, Emer, Flaatten, Hilde I., Flores, Gustavo E., Fuerch, Janene, Garg, Rakesh, Gately, Callum, Goh, Mark, Halamek, Louis P., Handley, Anthony J., Hatanaka, Tetsuo, Hoover, Amber, Issa, Mohmoud, Johnson, Samantha, Kamlin, C. Omar, Ko, Ying-Chih, Kule, Amy, Leone, Tina A., MacKenzie, Ella, Macneil, Finlay, Montgomery, William, O’Dochartaigh, Domhnall, Ohshimo, Shinichiro, Stefano Palazzo, Francesco, Picard, Christopher, Quek, Bin Huey, Raitt, James, Ramaswamy, Viraraghavan V., Scapigliati, Andrea, Shah, Birju A., Stewart, Craig, Strand, Marya L., Szyld, Edgardo, Thio, Marta, Topjian, Alexis A., Udaeta, Enrique, Vaillancourt, Christian, Wetsch, Wolfgang A., Wigginton, Jane, Yamada, Nicole K., Yao, Sarah, Zace, Drieda, Zelop, Carolyn M., Wyckoff, Myra H., Greif, Robert, Morley, Peter T., Ng, Kee-Chong, Olasveengen, Theresa M., Singletary, Eunice M., Soar, Jasmeet, Cheng, Adam, Drennan, Ian R., Liley, Helen G., Scholefield, Barnaby R., Smyth, Michael A., Welsford, Michelle, Zideman, David A., Acworth, Jason, Aickin, Richard, Andersen, Lars W., Atkins, Diane, Berry, David C., Bhanji, Farhan, Bierens, Joost, Borra, Vere, Böttiger, Bernd W., Bradley, Richard N., Bray, Janet E., Breckwoldt, Jan, Callaway, Clifton W., Carlson, Jestin N., Cassan, Pascal, Castrén, Maaret, Chang, Wei-Tien, Charlton, Nathan P., Phil Chung, Sung, Considine, Julie, Costa-Nobre, Daniela T., Couper, Keith, Couto, Thomaz Bittencourt, Dainty, Katie N., Davis, Peter G., de Almeida, Maria Fernanda, de Caen, Allan R., Deakin, Charles D., Djärv, Therese, Donnino, Michael W., Douma, Matthew J., Duff, Jonathan P., Dunne, Cody L., Eastwood, Kathryn, El-Naggar, Walid, Fabres, Jorge G., Fawke, Joe, Finn, Judith, Foglia, Elizabeth E., Folke, Fredrik, Gilfoyle, Elaine, Goolsby, Craig A., Granfeldt, Asger, Guerguerian, Anne-Marie, Guinsburg, Ruth, Hirsch, Karen G., Holmberg, Mathias J., Hosono, Shigeharu, Hsieh, Ming-Ju, Hsu, Cindy H., Ikeyama, Takanari, Isayama, Tetsuya, Johnson, Nicholas J., Kapadia, Vishal S., Kawakami, Mandira Daripa, Kim, Han-Suk, Kleinman, Monica, Kloeck, David A., Kudenchuk, Peter J., Lagina, Anthony T., Lauridsen, Kasper G., Lavonas, Eric J., Lee, Henry C., Lin, Yiqun (Jeffrey), Lockey, Andrew S., Maconochie, Ian K., Madar, R. John, Malta Hansen, Carolina, Masterson, Siobhan, Matsuyama, Tasuku, McKinlay, Christopher J.D., Meyran, Daniel, Morgan, Patrick, Morrison, Laurie J., Nadkarni, Vinay, Nakwa, Firdose L., Nation, Kevin J., Nehme, Ziad, Nemeth, Michael, Neumar, Robert W., Nicholson, Tonia, Nikolaou, Nikolaos, Nishiyama, Chika, Norii, Tatsuya, Nuthall, Gabrielle A., O’Neill, Brian J., Gene Ong, Yong-Kwang, Orkin, Aaron M., Paiva, Edison F., Parr, Michael J., Patocka, Catherine, Pellegrino, Jeffrey L., Perkins, Gavin D., Perlman, Jeffrey M., Rabi, Yacov, Reis, Amelia G., Reynolds, Joshua C., Ristagno, Giuseppe, Rodriguez-Nunez, Antonio, Roehr, Charles C., Rüdiger, Mario, Sakamoto, Tetsuya, Sandroni, Claudio, Sawyer, Taylor L., Schexnayder, Steve M., Schmölzer, Georg M., Schnaubelt, Sebastian, Semeraro, Federico, Skrifvars, Markus B., Smith, Christopher M., Sugiura, Takahiro, Tijssen, Janice A., Trevisanuto, Daniele, Van de Voorde, Patrick, Wang, Tzong-Luen, Weiner, Gary M., Wyllie, Jonathan P., Yang, Chih-Wei, Yeung, Joyce, Nolan, Jerry P., and Berg, Katherine M.

Published
2022
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19. CD163+ macrophages restrain vascular calcification, promoting the development of high-risk plaque

Author

Atsushi Sakamoto, Rika Kawakami, Masayuki Mori, Liang Guo, Ka Hyun Paek, Jose Verdezoto Mosquera, Anne Cornelissen, Saikat Kumar B. Ghosh, Kenji Kawai, Takao Konishi, Raquel Fernandez, Daniela T. Fuller, Weili Xu, Aimee E. Vozenilek, Yu Sato, Hiroyuki Jinnouchi, Sho Torii, Adam W. Turner, Hirokuni Akahori, Salome Kuntz, Craig C. Weinkauf, Parker J. Lee, Robert Kutys, Kathryn Harris, Alfred Lawrence Killey, Christina M. Mayhew, Matthew Ellis, Leah M. Weinstein, Neel V. Gadhoke, Roma Dhingra, Jeremy Ullman, Armella Dikongue, Maria E. Romero, Frank D. Kolodgie, Clint L. Miller, Renu Virmani, and Aloke V. Finn

Subjects
Cell biology, Vascular biology, Medicine
Abstract

Vascular calcification (VC) is concomitant with atherosclerosis, yet it remains uncertain why rupture-prone high-risk plaques do not typically show extensive calcification. Intraplaque hemorrhage (IPH) deposits erythrocyte-derived cholesterol, enlarging the necrotic core and promoting high-risk plaque development. Pro-atherogenic CD163+ alternative macrophages engulf hemoglobin:haptoglobin (HH) complexes at IPH sites. However, their role in VC has never been examined to our knowledge. Here we show, in human arteries, the distribution of CD163+ macrophages correlated inversely with VC. In vitro experiments using vascular smooth muscle cells (VSMCs) cultured with HH-exposed human macrophage — M(Hb) — supernatant reduced calcification, while arteries from ApoE–/– CD163–/– mice showed greater VC. M(Hb) supernatant–exposed VSMCs showed activated NF-κB, while blocking NF-κB attenuated the anticalcific effect of M(Hb) on VSMCs. CD163+ macrophages altered VC through NF-κB–induced transcription of hyaluronan synthase (HAS), an enzyme that catalyzes the formation of the extracellular matrix glycosaminoglycan, hyaluronan, within VSMCs. M(Hb) supernatants enhanced HAS production in VSMCs, while knocking down HAS attenuated its anticalcific effect. NF-κB blockade in ApoE–/– mice reduced hyaluronan and increased VC. In human arteries, hyaluronan and HAS were increased in areas of CD163+ macrophage presence. Our findings highlight an important mechanism by which CD163+ macrophages inhibit VC through NF-κB–induced HAS augmentation and thus promote the high-risk plaque development.

Published
2023
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20. Analysis of Swing Oscillatory Motion

Author

Nikolov, Svetoslav G., Vassilev, Vassil M., Zaharieva, Daniela T., Kacprzyk, Janusz, Series Editor, Georgiev, Ivan, editor, Kostadinov, Hristo, editor, and Lilkova, Elena, editor

Published
2021
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22. NFIB Haploinsufficiency Is Associated with Intellectual Disability and Macrocephaly

Author

Schanze, Ina, Bunt, Jens, Lim, Jonathan WC, Schanze, Denny, Dean, Ryan J, Alders, Marielle, Blanchet, Patricia, Attié-Bitach, Tania, Berland, Siren, Boogert, Steven, Boppudi, Sangamitra, Bridges, Caitlin J, Cho, Megan T, Dobyns, William B, Donnai, Dian, Douglas, Jessica, Earl, Dawn L, Edwards, Timothy J, Faivre, Laurence, Fregeau, Brieana, Genevieve, David, Gérard, Marion, Gatinois, Vincent, Holder-Espinasse, Muriel, Huth, Samuel F, Izumi, Kosuke, Kerr, Bronwyn, Lacaze, Elodie, Lakeman, Phillis, Mahida, Sonal, Mirzaa, Ghayda M, Morgan, Sian M, Nowak, Catherine, Peeters, Hilde, Petit, Florence, Pilz, Daniela T, Puechberty, Jacques, Reinstein, Eyal, Rivière, Jean-Baptiste, Santani, Avni B, Schneider, Anouck, Sherr, Elliott H, Smith-Hicks, Constance, Wieland, Ilse, Zackai, Elaine, Zhao, Xiaonan, Gronostajski, Richard M, Zenker, Martin, and Richards, Linda J

Subjects
Biomedical and Clinical Sciences, Mental Health, Pediatric, Intellectual and Developmental Disabilities (IDD), Neurosciences, Rare Diseases, Behavioral and Social Science, Genetics, Brain Disorders, Aetiology, 2.1 Biological and endogenous factors, Mental health, Neurological, Adolescent, Adult, Animals, Cerebral Cortex, Child, Child, Preschool, Codon, Nonsense, Cohort Studies, Corpus Callosum, Female, Haploinsufficiency, Humans, Intellectual Disability, Male, Megalencephaly, Mice, Mice, Knockout, NFI Transcription Factors, Polymorphism, Single Nucleotide, Young Adult, NFIB, agenesis of the corpus callosum, chromosome 9p22.3, chromosome 9p23, developmental delay, haploinsufficiency, intellectual disability, macrocephaly, megalencephaly, nuclear factor I, Biological Sciences, Medical and Health Sciences, Genetics & Heredity, Biological sciences, Biomedical and clinical sciences, Health sciences
Abstract

The nuclear factor I (NFI) family of transcription factors play an important role in normal development of multiple organs. Three NFI family members are highly expressed in the brain, and deletions or sequence variants in two of these, NFIA and NFIX, have been associated with intellectual disability (ID) and brain malformations. NFIB, however, has not previously been implicated in human disease. Here, we present a cohort of 18 individuals with mild ID and behavioral issues who are haploinsufficient for NFIB. Ten individuals harbored overlapping microdeletions of the chromosomal 9p23-p22.2 region, ranging in size from 225 kb to 4.3 Mb. Five additional subjects had point sequence variations creating a premature termination codon, and three subjects harbored single-nucleotide variations resulting in an inactive protein as determined using an in vitro reporter assay. All individuals presented with additional variable neurodevelopmental phenotypes, including muscular hypotonia, motor and speech delay, attention deficit disorder, autism spectrum disorder, and behavioral abnormalities. While structural brain anomalies, including dysgenesis of corpus callosum, were variable, individuals most frequently presented with macrocephaly. To determine whether macrocephaly could be a functional consequence of NFIB disruption, we analyzed a cortex-specific Nfib conditional knockout mouse model, which is postnatally viable. Utilizing magnetic resonance imaging and histology, we demonstrate that Nfib conditional knockout mice have enlargement of the cerebral cortex but preservation of overall brain structure and interhemispheric connectivity. Based on our findings, we propose that haploinsufficiency of NFIB causes ID with macrocephaly.

Published
2018

24. 2022 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations: Summary From the Basic Life Support; Advanced Life Support; Pediatric Life Support; Neonatal Life Support; Education, Implementation, and Teams; and First Aid Task Forces

Author

Wyckoff, Myra H., Greif, Robert, Morley, Peter T., Ng, Kee-Chong, Olasveengen, Theresa M., Singletary, Eunice M., Soar, Jasmeet, Cheng, Adam, Drennan, Ian R., Liley, Helen G., Scholefield, Barnaby R., Smyth, Michael A., Welsford, Michelle, Zideman, David A., Acworth, Jason, Aickin, Richard, Andersen, Lars W., Atkins, Diane, Berry, David C., Bhanji, Farhan, Bierens, Joost, Borra, Vere, Böttiger, Bernd W., Bradley, Richard N., Bray, Janet E., Breckwoldt, Jan, Callaway, Clifton W., Carlson, Jestin N., Cassan, Pascal, Castrén, Maaret, Chang, Wei-Tien, Charlton, Nathan P., Chung, Sung Phil, Considine, Julie, Costa-Nobre, Daniela T., Couper, Keith, Couto, Thomaz Bittencourt, Dainty, Katie N., Davis, Peter G., de Almeida, Maria Fernanda, de Caen, Allan R., Deakin, Charles D., Djärv, Therese, Donnino, Michael W., Douma, Matthew J., Duff, Jonathan P., Dunne, Cody L., Eastwood, Kathryn, El-Naggar, Walid, Fabres, Jorge G., Fawke, Joe, Finn, Judith, Foglia, Elizabeth E., Folke, Fredrik, Gilfoyle, Elaine, Goolsby, Craig A., Granfeldt, Asger, Guerguerian, Anne-Marie, Guinsburg, Ruth, Hirsch, Karen G., Holmberg, Mathias J., Hosono, Shigeharu, Hsieh, Ming-Ju, Hsu, Cindy H., Ikeyama, Takanari, Isayama, Tetsuya, Johnson, Nicholas J., Kapadia, Vishal S., Kawakami, Mandira Daripa, Kim, Han-Suk, Kleinman, Monica, Kloeck, David A., Kudenchuk, Peter J., Lagina, Anthony T., Lauridsen, Kasper G., Lavonas, Eric J., Lee, Henry C., Lin, Yiqun (Jeffrey), Lockey, Andrew S., Maconochie, Ian K., Madar, R. John, Malta Hansen, Carolina, Masterson, Siobhan, Matsuyama, Tasuku, McKinlay, Christopher J.D., Meyran, Daniel, Morgan, Patrick, Morrison, Laurie J., Nadkarni, Vinay, Nakwa, Firdose L., Nation, Kevin J., Nehme, Ziad, Nemeth, Michael, Neumar, Robert W., Nicholson, Tonia, Nikolaou, Nikolaos, Nishiyama, Chika, Norii, Tatsuya, Nuthall, Gabrielle A., O’Neill, Brian J., Ong, Yong-Kwang Gene, Orkin, Aaron M., Paiva, Edison F., Parr, Michael J., Patocka, Catherine, Pellegrino, Jeffrey L., Perkins, Gavin D., Perlman, Jeffrey M., Rabi, Yacov, Reis, Amelia G., Reynolds, Joshua C., Ristagno, Giuseppe, Rodriguez-Nunez, Antonio, Roehr, Charles C., Rüdiger, Mario, Sakamoto, Tetsuya, Sandroni, Claudio, Sawyer, Taylor L., Schexnayder, Steve M., Schmölzer, Georg M., Schnaubelt, Sebastian, Semeraro, Federico, Skrifvars, Markus B., Smith, Christopher M., Sugiura, Takahiro, Tijssen, Janice A., Trevisanuto, Daniele, Van de Voorde, Patrick, Wang, Tzong-Luen, Weiner, Gary M., Wyllie, Jonathan P., Yang, Chih-Wei, Yeung, Joyce, Nolan, Jerry P., Berg, Katherine M., Burdick, Madeline C., Cartledge, Susie, Dawson, Jennifer A., Elgohary, Moustafa M., Ersdal, Hege L., Finan, Emer, Flaatten, Hilde I., Flores, Gustavo E., Fuerch, Janene, Garg, Rakesh, Gately, Callum, Goh, Mark, Halamek, Louis P., Handley, Anthony J., Hatanaka, Tetsuo, Hoover, Amber, Issa, Mohmoud, Johnson, Samantha, Kamlin, C. Omar, Ko, Ying-Chih, Kule, Amy, Leone, Tina A., MacKenzie, Ella, Macneil, Finlay, Montgomery, William, O’Dochartaigh, Domhnall, Ohshimo, Shinichiro, Palazzo, Francesco Stefano, Picard, Christopher, Quek, Bin Huey, Raitt, James, Ramaswamy, Viraraghavan V., Scapigliati, Andrea, Shah, Birju A., Stewart, Craig, Strand, Marya L., Szyld, Edgardo, Thio, Marta, Topjian, Alexis A., Udaeta, Enrique, Vaillancourt, Christian, Wetsch, Wolfgang A., Wigginton, Jane, Yamada, Nicole K., Yao, Sarah, Zace, Drieda, and Zelop, Carolyn M.

Published
2022
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26. Single-cell analysis shows that adipose tissue of persons with both HIV and diabetes is enriched for clonal, cytotoxic, and CMV-specific CD4+ T cells

Author

Wanjalla, Celestine N., McDonnell, Wyatt J., Ram, Ramesh, Chopra, Abha, Gangula, Rama, Leary, Shay, Mashayekhi, Mona, Simmons, Joshua D., Warren, Christian M., Bailin, Samuel, Gabriel, Curtis L., Guo, Liang, Furch, Briana D., Lima, Morgan C., Woodward, Beverly O., Hannah, LaToya, Pilkinton, Mark A., Fuller, Daniela T., Kawai, Kenji, Virmani, Renu, Finn, Aloke V., Hasty, Alyssa H., Mallal, Simon A., Kalams, Spyros A., and Koethe, John R.

Published
2021
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29. Temporal Trend, Causes, and Timing of Neonatal Mortality of Moderate and Late Preterm Infants in São Paulo State, Brazil: A Population-Based Study

Author

Maria Fernanda B. de Almeida, Adriana Sanudo, Kelsy N. Areco, Rita de Cássia X. Balda, Daniela T. Costa-Nobre, Mandira D. Kawakami, Tulio Konstantyner, Ana Sílvia S. Marinonio, Milton H. Miyoshi, Paulo Bandiera-Paiva, Rosa M. V. Freitas, Liliam C. C. Morais, Mônica L. P. Teixeira, Bernadette Waldvogel, Carlos Roberto V. Kiffer, and Ruth Guinsburg

Subjects
infant newborn, infant, premature, neonatal mortality, cause of death, developing countries, Pediatrics, RJ1-570
Abstract

Moderate and late preterm newborns comprise around 85% of live births < 37 weeks gestation. Data on their neonatal mortality in middle-income countries is limited. This study aims to analyze the temporal trend, causes and timing of neonatal mortality of infants with 320/7–366/7 weeks gestation without congenital anomalies from 2004–2015 in the population of São Paulo State, Brazil. A database was built by deterministic linkage of birth and death certificates. Causes of death were classified by ICD-10 codes. Among 7,317,611 live births in the period, there were 545,606 infants with 320/7–366/7 weeks gestation without congenital anomalies, and 5782 of them died between 0 and 27 days. The neonatal mortality rate decreased from 16.4 in 2004 to 7.6 per thousand live births in 2015 (7.47% annual decrease by Prais–Winsten model). Perinatal asphyxia, respiratory disorders and infections were responsible, respectively, for 14%, 27% and 44% of the 5782 deaths. Median time to death was 24, 53 and 168 h, respectively, for perinatal asphyxia, respiratory disorders, and infections. Bottlenecks in perinatal health care are probably associated with the results that indicate the need for policies to reduce preventable neonatal deaths of moderate and late preterm infants in the most developed state of Brazil.

Published
2023
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30. Annual trend of neonatal mortality and its underlying causes: population-based study – São Paulo State, Brazil, 2004–2013

Author

Ruth Guinsburg, Adriana Sanudo, Carlos Roberto V Kiffer, Ana Sílvia S Marinonio, Daniela T Costa-Nobre, Kelsy N Areco, Mandira D Kawakami, Milton H Miyoshi, Paulo Bandiera-Paiva, Rita de Cássia X Balda, Tulio Konstantyner, Liliam CC Morais, Rosa MV Freitas, Mônica LP Teixeira, Bernadette Waldvogel, and Maria Fernanda B Almeida

Subjects
Infant newborn, Neonatal mortality, Infant, premature, Developing countries, Epidemiological studies, Pediatrics, RJ1-570
Abstract

Abstract Background Population-based studies analyzing neonatal deaths in middle-income countries may contribute to design interventions to achieve the Sustainable Development Goals, established by United Nations. This study goal is to analyze the annual trend of neonatal mortality in São Paulo State, Brazil, over a 10-year period and its underlying causes and to identify maternal and neonatal characteristics at birth associated with neonatal mortality. Method A population-based study of births and deaths from 0 to 27 days between 2004 and 2013 in São Paulo State, Brazil, was performed. The annual trend of neonatal mortality rate according to gestational age was analyzed by Poisson or by Negative Binomial Regression models. Basic causes of neonatal death were classified according to ICD-10. Association of maternal demographic variables (block 1), prenatal and delivery care variables (block 2), and neonatal characteristics at birth (block 3) with neonatal mortality was evaluated by Poisson regression analysis adjusted by year of birth. Results Among 6,056,883 live births in São Paulo State during the study period, 48,309 died from 0 to 27 days (neonatal mortality rate: 8.0/1,000 live births). For the whole group and for infants with gestational age 22–27, 28–31, 32–36, 37–41 and ≥ 42 weeks, reduction of neonatal mortality rate was, respectively, 18 %, 15 %, 38 %, 53 %, 31 %, and 58 %. Median time until 50 % of deaths occurred was 3 days. Main basic causes of death were respiratory disorders (25 %), malformations (20 %), infections (17 %), and perinatal asphyxia (7 %). Variables independently associated with neonatal deaths were maternal schooling, prenatal care, parity, newborn sex, 1st minute Apgar, and malformations. Cesarean delivery, compared to vaginal, was protective against neonatal mortality for infants at 22–31 weeks, but it was a risk factor for those with 32–41 weeks. Conclusions Despite the significant decrease in neonatal mortality rate over the 10-year period in São Paulo State, improved access to qualified health care is needed in order to avoid preventable neonatal deaths and increase survival of infants that need more complex levels of assistance.

Published
2021
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31. Lessons for a SECURE Future: Evaluating Diversity in Crop Biotechnology Across Regulatory Regimes

Author

Dalton R. George, Eli D. Hornstein, Carrie A. Clower, Allison L. Coomber, DeShae Dillard, Nassib Mugwanya, Daniela T. Pezzini, and Casey Rozowski

Subjects
crop biotechnology, SECURE rule, regulation, diversity trends, innovation, United States, Biotechnology, TP248.13-248.65
Abstract

Regulation of next-generation crops in the United States under the newly implemented “SECURE” rule promises to diversify innovation in agricultural biotechnology. Specifically, SECURE promises to expand the number of products eligible for regulatory exemption, which proponents theorize will increase the variety of traits, genes, organisms, and developers involved in developing crop biotechnology. However, few data-driven studies have looked back at the history of crop biotechnology to understand how specific regulatory pathways have affected diversity in crop biotechnology and how those patterns might change over time. In this article, we draw upon 30 years of regulatory submission data to 1) understand historical diversification trends across the landscape and history of past crop biotechnology regulatory pathways and 2) forecast how the new SECURE regulations might affect future diversification trends. Our goal is to apply an empirical approach to exploring the relationship between regulation and diversity in crop biotechnology and provide a basis for future data-driven analysis of regulatory outcomes. Based on our analysis, we suggest that diversity in crop biotechnology does not follow a single trajectory dictated by the shifts in regulation, and outcomes of SECURE might be more varied and restrictive despite the revamped exemption categories. In addition, the concept of confidential business information and its relationship to past and future biotechnology regulation is reviewed in light of our analysis.

Published
2022
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33. 2023 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations: Summary From the Basic Life Support; Advanced Life Support; Pediatric Life Support; Neonatal Life Support; Education, Implementation, and Teams; and First Aid Task Forces

Author

Berg, Katherine M., primary, Bray, Janet E., additional, Ng, Kee-Chong, additional, Liley, Helen G., additional, Greif, Robert, additional, Carlson, Jestin N., additional, Morley, Peter T., additional, Drennan, Ian R., additional, Smyth, Michael, additional, Scholefield, Barnaby R., additional, Weiner, Gary M., additional, Cheng, Adam, additional, Djärv, Therese, additional, Abelairas-Gómez, Cristian, additional, Acworth, Jason, additional, Andersen, Lars W., additional, Atkins, Dianne L., additional, Berry, David C., additional, Bhanji, Farhan, additional, Bierens, Joost, additional, Bittencourt Couto, Thomaz, additional, Borra, Vere, additional, Böttiger, Bernd W., additional, Bradley, Richard N., additional, Breckwoldt, Jan, additional, Cassan, Pascal, additional, Chang, Wei-Tien, additional, Charlton, Nathan P., additional, Chung, Sung Phil, additional, Considine, Julie, additional, Costa-Nobre, Daniela T., additional, Couper, Keith, additional, Dainty, Katie N., additional, Dassanayake, Vihara, additional, Davis, Peter G., additional, Dawson, Jennifer A., additional, Fernanda de Almeida, Maria, additional, De Caen, Allan R., additional, Deakin, Charles D., additional, Dicker, Bridget, additional, Douma, Matthew J., additional, Eastwood, Kathryn, additional, El-Naggar, Walid, additional, Fabres, Jorge G., additional, Fawke, Joe, additional, Fijacko, Nino, additional, Finn, Judith C., additional, Flores, Gustavo E., additional, Foglia, Elizabeth E., additional, Folke, Fredrik, additional, Gilfoyle, Elaine, additional, Goolsby, Craig A., additional, Granfeldt, Asger, additional, Guerguerian, Anne-Marie, additional, Guinsburg, Ruth, additional, Hatanaka, Tetsuo, additional, Hirsch, Karen G., additional, Holmberg, Mathias J., additional, Hosono, Shigeharu, additional, Hsieh, Ming-Ju, additional, Hsu, Cindy H., additional, Ikeyama, Takanari, additional, Isayama, Tetsuya, additional, Johnson, Nicholas J., additional, Kapadia, Vishal S., additional, Daripa Kawakami, Mandira, additional, Kim, Han-Suk, additional, Kleinman, Monica E., additional, Kloeck, David A., additional, Kudenchuk, Peter, additional, Kule, Amy, additional, Kurosawa, Hiroshi, additional, Lagina, Anthony T., additional, Lauridsen, Kasper G., additional, Lavonas, Eric J., additional, Lee, Henry C., additional, Lin, Yiqun, additional, Lockey, Andrew S., additional, Macneil, Finlay, additional, Maconochie, Ian K., additional, John Madar, R., additional, Malta Hansen, Carolina, additional, Masterson, Siobhan, additional, Matsuyama, Tasuku, additional, McKinlay, Christopher J.D., additional, Meyran, Daniel, additional, Monnelly, Vix, additional, Nadkarni, Vinay, additional, Nakwa, Firdose L., additional, Nation, Kevin J., additional, Nehme, Ziad, additional, Nemeth, Michael, additional, Neumar, Robert W., additional, Nicholson, Tonia, additional, Nikolaou, Nikolaos, additional, Nishiyama, Chika, additional, Norii, Tatsuya, additional, Nuthall, Gabrielle A., additional, Ohshimo, Shinchiro, additional, Olasveengen, Theresa M., additional, Gene Ong, Yong-Kwang, additional, Orkin, Aaron M., additional, Parr, Michael J., additional, Patocka, Catherine, additional, Perkins, Gavin D., additional, Perlman, Jeffrey M., additional, Rabi, Yacov, additional, Raitt, James, additional, Ramachandran, Shalini, additional, Ramaswamy, Viraraghavan V., additional, Raymond, Tia T., additional, Reis, Amelia G., additional, Reynolds, Joshua C., additional, Ristagno, Giuseppe, additional, Rodriguez-Nunez, Antonio, additional, Roehr, Charles C., additional, Rüdiger, Mario, additional, Sakamoto, Tetsuya, additional, Sandroni, Claudio, additional, Sawyer, Taylor L., additional, Schexnayder, Steve M., additional, Schmölzer, Georg M., additional, Schnaubelt, Sebastian, additional, Semeraro, Federico, additional, Singletary, Eunice M., additional, Skrifvars, Markus B., additional, Smith, Christopher M., additional, Soar, Jasmeet, additional, Stassen, Willem, additional, Sugiura, Takahiro, additional, Tijssen, Janice A., additional, Topjian, Alexis A., additional, Trevisanuto, Daniele, additional, Vaillancourt, Christian, additional, Wyckoff, Myra H., additional, Wyllie, Jonathan P., additional, Yang, Chih-Wei, additional, Yeung, Joyce, additional, Zelop, Carolyn M., additional, Zideman, David A., additional, Nolan, Jerry P., additional, Barcala-Furelos, Roberto, additional, Beerman, Stephen B., additional, Bruckner, Marlies, additional, Castrén, Maaret, additional, Chong, ShuLing, additional, Claesson, Andreas, additional, Dunne, Cody L., additional, Finan, Emer, additional, Fukuda, Tatsuma, additional, Lalgudi Ganesan, Saptharishi, additional, Gately, Callum, additional, Gois, Aecio, additional, Gray, Seth, additional, Halamek, Louis P., additional, Hoover, Amber V., additional, Hurst, Cameron, additional, Josephsen, Justin, additional, Kollander, Louise, additional, Omar Kamlin, C., additional, Kool, Mirjam, additional, Li, Lei, additional, Mecrow, Thomas S., additional, Montgomery, William, additional, Ristau, Patrick, additional, Jayashree, Muralidharan, additional, Schmidt, Andrew, additional, Scquizzato, Tommaso, additional, Seesink, Jeroen, additional, Sempsrott, Justin, additional, Lee Solevåg, Anne, additional, Strand, Marya L., additional, Szpilman, David, additional, Szyld, Edgardo, additional, Thom, Ogilvie, additional, Tobin, Joshua M., additional, Trang, Jacinta, additional, Webber, Jonathon, additional, Webster, Hannah K., additional, and Wellsford, Michelle, additional

Published
2024
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34. Effect of physical activity on the relationship between adiposity and cardiac autonomic modulation in female breast cancer survivors: a longitudinal study

Author

Palma, Mariana R., primary, Tebar, William R., additional, Beretta, Victor S., additional, Vanderlei, Luiz Carlos M., additional, Fregonesi, Cristina E. P. T., additional, Ribeiro, Fernanda E., additional, Caldeira, Daniela T., additional, Ramos, Ercy M. C., additional, Cucato, Gabriel G., additional, Ferrari, Gerson, additional, Ritti-Dias, Raphael M., additional, and Christofaro, Diego G. D., additional

Published
2024
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36. Annual trend of neonatal mortality and its underlying causes: population-based study – São Paulo State, Brazil, 2004–2013

Author

Guinsburg, Ruth, Sanudo, Adriana, Kiffer, Carlos Roberto V, Marinonio, Ana Sílvia S, Costa-Nobre, Daniela T, Areco, Kelsy N, Kawakami, Mandira D, Miyoshi, Milton H, Bandiera-Paiva, Paulo, Balda, Rita de Cássia X, Konstantyner, Tulio, Morais, Liliam CC, Freitas, Rosa MV, Teixeira, Mônica LP, Waldvogel, Bernadette, and Almeida, Maria Fernanda B

Published
2021
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37. 2021 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations: Summary From the Basic Life Support; Advanced Life Support; Neonatal Life Support; Education, Implementation, and Teams; First Aid Task Forces; and the COVID-19 Working Group

Author

Wyckoff, Myra H., Singletary, Eunice M., Soar, Jasmeet, Olasveengen, Theresa M., Greif, Robert, Liley, Helen G., Zideman, David, Bhanji, Farhan, Andersen, Lars W., Avis, Suzanne R., Aziz, Khalid, Bendall, Jason C., Berry, David C., Borra, Vere, Böttiger, Bernd W., Bradley, Richard, Bray, Janet E., Breckwoldt, Jan, Carlson, Jestin N., Cassan, Pascal, Castrén, Maaret, Chang, Wei-Tien, Charlton, Nathan P., Cheng, Adam, Chung, Sung Phil, Considine, Julie, Costa-Nobre, Daniela T., Couper, Keith, Dainty, Katie N., Davis, Peter G., de Almeida, Maria Fernanda, de Caen, Allan R., de Paiva, Edison F., Deakin, Charles D., Djärv, Therese, Douma, Matthew J., Drennan, Ian R., Duff, Jonathan P., Eastwood, Kathryn J., Epstein, Jonathan L., Escalante, Raffo, Fabres, Jorge G., Fawke, Joe, Finn, Judith C., Foglia, Elizabeth E., Folke, Fredrik, Freeman, Karoline, Gilfoyle, Elaine, Goolsby, Craig A., Grove, Amy, Guinsburg, Ruth, Hatanaka, Tetsuo, Hazinski, Mary Fran, Heriot, George S., Hirsch, Karen G., Holmberg, Mathias J., Hosono, Shigeharu, Hsieh, Ming-Ju, Hung, Kevin K.C., Hsu, Cindy H., Ikeyama, Takanari, Isayama, Tetsuya, Kapadia, Vishal S., Kawakami, Mandira, Kim, Han-Suk, Kloeck, David A., Kudenchuk, Peter J., Lagina, Anthony T., Lauridsen, Kasper G., Lavonas, Eric J., Lockey, Andrew S., Malta Hansen, Carolina, Markenson, David, Matsuyama, Tasuku, McKinlay, Christopher J.D., Mehrabian, Amin, Merchant, Raina M., Meyran, Daniel, Morley, Peter T., Morrison, Laurie J., Nation, Kevin J., Nemeth, Michael, Neumar, Robert W., Nicholson, Tonia, Niermeyer, Susan, Nikolaou, Nikolaos, Nishiyama, Chika, O’Neil, Brian J., Orkin, Aaron M., Osemeke, Osokogu, Parr, Michael J., Patocka, Catherine, Pellegrino, Jeffrey L., Perkins, Gavin D., Perlman, Jeffrey M., Rabi, Yacov, Reynolds, Joshua C., Ristagno, Giuseppe, Roehr, Charles C., Sakamoto, Tetsuya, Sandroni, Claudio, Sawyer, Taylor, Schmölzer, Georg M., Schnaubelt, Sebastian, Semeraro, Federico, Skrifvars, Markus B., Smith, Christopher M., Smyth, Michael A., Soll, Roger F., Sugiura, Takahiro, Taylor-Phillips, Sian, Trevisanuto, Daniele, Vaillancourt, Christian, Wang, Tzong-Luen, Weiner, Gary M., Welsford, Michelle, Wigginton, Jane, Wyllie, Jonathan P., Yeung, Joyce, Nolan, Jerry P., and Berg, Katherine M.

Published
2021
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38. International consensus recommendations on the diagnostic work-up for malformations of cortical development

Author

Oegema, Renske, Barakat, Tahsin Stefan, Wilke, Martina, Stouffs, Katrien, Amrom, Dina, Aronica, Eleonora, Bahi-Buisson, Nadia, Conti, Valerio, Fry, Andrew E., Geis, Tobias, Andres, David Gomez, Parrini, Elena, Pogledic, Ivana, Said, Edith, Soler, Doriette, Valor, Luis M., Zaki, Maha S., Mirzaa, Ghayda, Dobyns, William B., Reiner, Orly, Guerrini, Renzo, Pilz, Daniela T., Hehr, Ute, Leventer, Richard J., Jansen, Anna C., Mancini, Grazia M. S., and Di Donato, Nataliya

Published
2020
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42. Operational Challenges in the Use of Structured Secondary Data for Health Research

Author

Kelsy N. Areco, Tulio Konstantyner, Paulo Bandiera-Paiva, Rita C. X. Balda, Daniela T. Costa-Nobre, Adriana Sanudo, Carlos Roberto V. Kiffer, Mandira D. Kawakami, Milton H. Miyoshi, Ana Sílvia Scavacini Marinonio, Rosa M. V. Freitas, Liliam C. C. Morais, Monica L. P. Teixeira, Bernadette Waldvogel, Maria Fernanda B. Almeida, and Ruth Guinsburg

Subjects
public health, datasets as topic, population studies in public health, Death Certificates, Birth Certificates, secondary health data, Public aspects of medicine, RA1-1270
Abstract

Background: In Brazil, secondary data for epidemiology are largely available. However, they are insufficiently prepared for use in research, even when it comes to structured data since they were often designed for other purposes. To date, few publications focus on the process of preparing secondary data. The present findings can help in orienting future research projects that are based on secondary data.Objective: Describe the steps in the process of ensuring the adequacy of a secondary data set for a specific use and to identify the challenges of this process.Methods: The present study is qualitative and reports methodological issues about secondary data use. The study material was comprised of 6,059,454 live births and 73,735 infant death records from 2004 to 2013 of children whose mothers resided in the State of São Paulo - Brazil. The challenges and description of the procedures to ensure data adequacy were undertaken in 6 steps: (1) problem understanding, (2) resource planning, (3) data understanding, (4) data preparation, (5) data validation and (6) data distribution. For each step, procedures, and challenges encountered, and the actions to cope with them and partial results were described. To identify the most labor-intensive tasks in this process, the steps were assessed by adding the number of procedures, challenges, and coping actions. The highest values were assumed to indicate the most critical steps.Results: In total, 22 procedures and 23 actions were needed to deal with the 27 challenges encountered along the process of ensuring the adequacy of the study material for the intended use. The final product was an organized database for a historical cohort study suitable for the intended use. Data understanding and data preparation were identified as the most critical steps, accounting for about 70% of the challenges observed for data using.Conclusion: Significant challenges were encountered in the process of ensuring the adequacy of secondary health data for research use, mainly in the data understanding and data preparation steps. The use of the described steps to approach structured secondary data and the knowledge of the potential challenges along the process may contribute to planning health research.

Published
2021
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44. De novo CCND2 mutations leading to stabilization of cyclin D2 cause megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome

Author

Mirzaa, Ghayda M, Parry, David A, Fry, Andrew E, Giamanco, Kristin A, Schwartzentruber, Jeremy, Vanstone, Megan, Logan, Clare V, Roberts, Nicola, Johnson, Colin A, Singh, Shawn, Kholmanskikh, Stanislav S, Adams, Carissa, Hodge, Rebecca D, Hevner, Robert F, Bonthron, David T, Braun, Kees PJ, Faivre, Laurence, Rivière, Jean-Baptiste, St-Onge, Judith, Gripp, Karen W, Mancini, Grazia MS, Pang, Ki, Sweeney, Elizabeth, van Esch, Hilde, Verbeek, Nienke, Wieczorek, Dagmar, Steinraths, Michelle, Majewski, Jacek, Boycott, Kym M, Pilz, Daniela T, Ross, M Elizabeth, Dobyns, William B, and Sheridan, Eamonn G

Subjects
Pediatric, 2.1 Biological and endogenous factors, Aetiology, Abnormalities, Multiple, Animals, Base Sequence, Blotting, Western, Bromodeoxyuridine, Cyclin D2, Electroporation, Exome, Female, HEK293 Cells, Humans, Hydrocephalus, Immunohistochemistry, Malformations of Cortical Development, Megalencephaly, Mice, Microscopy, Fluorescence, Molecular Sequence Data, Mutagenesis, Site-Directed, Polydactyly, Sequence Analysis, DNA, Syndrome, FORGE Canada Consortium, Biological Sciences, Medical and Health Sciences, Developmental Biology
Abstract

Activating mutations in genes encoding phosphatidylinositol 3-kinase (PI3K)-AKT pathway components cause megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome (MPPH, OMIM 603387). Here we report that individuals with MPPH lacking upstream PI3K-AKT pathway mutations carry de novo mutations in CCND2 (encoding cyclin D2) that are clustered around a residue that can be phosphorylated by glycogen synthase kinase 3β (GSK-3β). Mutant CCND2 was resistant to proteasomal degradation in vitro compared to wild-type CCND2. The PI3K-AKT pathway modulates GSK-3β activity, and cells from individuals with PIK3CA, PIK3R2 or AKT3 mutations showed similar CCND2 accumulation. CCND2 was expressed at higher levels in brains of mouse embryos expressing activated AKT3. In utero electroporation of mutant CCND2 into embryonic mouse brains produced more proliferating transfected progenitors and a smaller fraction of progenitors exiting the cell cycle compared to cells electroporated with wild-type CCND2. These observations suggest that cyclin D2 stabilization, caused by CCND2 mutation or PI3K-AKT activation, is a unifying mechanism in PI3K-AKT-related megalencephaly syndromes.

Published
2014

46. Contact Allergy in Atopic Patients

Author

Popov, Alexander K., primary, Gospodinova, Klimentina D., additional, Gincheva, Veronika H., additional, Grozeva, Daniela T., additional, and Gospodinov, Dimitar K., additional

Published
2023
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49. Comparative Study about the Consumption of Organic Food Products on Samples of Portuguese and Turkish Consumers under the COVID-19 Pandemic Context

Author

Raquel P. F. Guiné, Sofia G. Florença, Daniela T. V. A. Costa, Selda Çelik, Manuela Ferreira, Ana Paula Cardoso, Sümeyye Çetin, and Cristina A. Costa

Subjects
sustainable agriculture, organic farming, food consumption, questionnaire survey, COVID-19, Agriculture
Abstract

This research aimed to study consumers’ trends and the consumption of foods obtained through organic farming in two different countries, Portugal and Turkey. A questionnaire survey was used, applied through internet tools as a result of COVID-19 pandemic restrictions. For the treatment of data, we used basic statistics complemented with a tree classification, aimed at evaluating the influence of sociodemographic factors on the knowledge about this type of food. The results showed that the consumption patterns are relatively similar in both countries, with many participants consuming organic foods, especially vegetables and fruits, consuming them mostly two or three meals per week. The strongest motivations to consume organic foods include benefits for human health and lower environmental impacts, while the most substantial reason not to consume is the higher price. It was also found that in both countries, people have good knowledge about the advantages of organic foods over conventional ones. Finally, the perception of the value attributed by society to organic foods was considerably higher in Portugal. These results confirm the apparent trend of making more sustainable food choices, which is motivated by the perceived negative impact of conventional agriculture on ecosystems and health.

Published
2022
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50. Evaluation of oxygen therapy in adult patients in teaching hospital of Sergipe

Author

Bianca L. SANTOS, Maria L. BARROS, Gessica U. OLIVEIRA, Daniela T. OLIVEIRA, Lucimara M. ANDRADE, Simony M. SOARES, Geovanna C. CARDOSO, Milena M. XAVIER, Marcell C. SILVA, and Fábio J. AMORIM

Subjects
Public aspects of medicine, RA1-1270, Pharmacy and materia medica, RS1-441, Therapeutics. Pharmacology, RM1-950
Abstract

Objective: To evaluate oxygen therapy prescriptions for patients under clinical care admitted to a teaching hospital in Sergipe. Methods: This is a cross-sectional, descriptive, retrospective and quantitative study, in which the medical records and prescriptions of 28 patients using oxygen (O2) hospitalized between March and June 2021 were evaluated. to the indication of O2 and initial oxygen saturation (SatO2), and the prescriptions regarding the inclusion of O2 in the prescription, target saturation, O2 delivery device and initial flow or initial inspired O2 concentration. After data collection, a panel of experts was formed, composed of a pulmonologist, physical therapists and pharmacists to evaluate the findings and suggest interventions to be carried out. Results: Of the patients evaluated, 89.3% had an indication for oxygen therapy, but 72.4% of the medical records did not present information on initial SatO2. Of the sample analyzed, 53.6% did not have prescribed O2, 75.0% did not have a SatO2 target, 42.9% did not have an O2 delivery device, and 67.9% did not have the initial flow or inspired concentration of oxygen. initial O2. In addition, the nasal catheter was the most prescribed device (85.2%). The panel of experts suggested nine interventions to be carried out, which ranged from training professionals involved in the entire process of using medical oxygen, to modifying the current medical prescription model to meet the necessary requirements for a prescription of adequate oxygen, in order to promote the practice of rational use of oxygen and adjustments in the work process. Conclusion: The study showed that most of the items evaluated in the oxygen prescriptions presented non-compliance with international recommendations. These findings justify carrying out interventions with the care team.

Published
2022
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