Search

Your search keyword '"Daniel Seitz"' showing total 22 results
Start Over Author "Daniel Seitz"
22 results on '"Daniel Seitz"'

1. Synergistic interaction of hTGF-β3 with hBMP-6 promotes articular cartilage formation in chitosan scaffolds with hADSCs: implications for regenerative medicine

Author

Yijiang Huang, Daniel Seitz, Yan Chevalier, Peter E. Müller, Volkmar Jansson, and Roland M. Klar

Subjects
Chitosan, Promotion, Adipose-derived stem cell, Articular Chondrogenesis, Bone formation, hTGF-β3, Biotechnology, TP248.13-248.65
Abstract

Abstract Background Human TGF-β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matrices. However, in vivo data suggests that TGF-β3 and its other isoforms also induce endochondral and intramembranous osteogenesis in non-primate species to other mammals. Based on previously demonstrated improved articular cartilage induction by a using hTGF-β3 and hBMP-6 together on hADSC cultures and the interaction of TGF- β with matrix in vivo, the present study investigates the interaction of a chitosan scaffold as polyanionic polysaccharide with both growth factors. The study analyzes the difference between chondrogenic differentiation that leads to stable hyaline cartilage and the endochondral ossification route that ends in hypertrophy by extending the usual panel of investigated gene expression and stringent employment of quantitative PCR. Results By assessing the viability, proliferation, matrix formation and gene expression patterns it is shown that hTGF-β3 + hBMP-6 promotes improved hyaline articular cartilage formation in a chitosan scaffold in which ACAN with Col2A1 and not Col1A1 nor Col10A1 where highly expressed both at a transcriptional and translational level. Inversely, hTGF-β3 alone tended towards endochondral bone formation showing according protein and gene expression patterns. Conclusion These findings demonstrate that clinical therapies should consider using hTGF-β3 + hBMP-6 in articular cartilage regeneration therapies as the synergistic interaction of these morphogens seems to ensure and maintain proper hyaline articular cartilage matrix formation counteracting degeneration to fibrous tissue or ossification. These effects are produced by interaction of the growth factors with the polysaccharide matrix.

Published
2020
Full Text
View/download PDF

2. The SuperCam Instrument Suite on the NASA Mars 2020 Rover: Body Unit and Combined System Tests

Author

Roger C. Wiens, Sylvestre Maurice, Scott H. Robinson, Anthony E. Nelson, Philippe Cais, Pernelle Bernardi, Raymond T. Newell, Sam Clegg, Shiv K. Sharma, Steven Storms, Jonathan Deming, Darrel Beckman, Ann M. Ollila, Olivier Gasnault, Ryan B. Anderson, Yves André, S. Michael Angel, Gorka Arana, Elizabeth Auden, Pierre Beck, Joseph Becker, Karim Benzerara, Sylvain Bernard, Olivier Beyssac, Louis Borges, Bruno Bousquet, Kerry Boyd, Michael Caffrey, Jeffrey Carlson, Kepa Castro, Jorden Celis, Baptiste Chide, Kevin Clark, Edward Cloutis, Elizabeth C. Cordoba, Agnes Cousin, Magdalena Dale, Lauren Deflores, Dorothea Delapp, Muriel Deleuze, Matthew Dirmyer, Christophe Donny, Gilles Dromart, M. George Duran, Miles Egan, Joan Ervin, Cecile Fabre, Amaury Fau, Woodward Fischer, Olivier Forni, Thierry Fouchet, Reuben Fresquez, Jens Frydenvang, Denine Gasway, Ivair Gontijo, John Grotzinger, Xavier Jacob, Sophie Jacquinod, Jeffrey R. Johnson, Roberta A. Klisiewicz, James Lake, Nina Lanza, Javier Laserna, Jeremie Lasue, Stéphane Le Mouélic, Carey Legett, Richard Leveille, Eric Lewin, Guillermo Lopez-Reyes, Ralph Lorenz, Eric Lorigny, Steven P. Love, Briana Lucero, Juan Manuel Madariaga, Morten Madsen, Soren Madsen, Nicolas Mangold, Jose Antonio Manrique, J. P. Martinez, Jesus Martinez-Frias, Kevin P. McCabe, Timothy H. McConnochie, Justin M. McGlown, Scott M. McLennan, Noureddine Melikechi, Pierre-Yves Meslin, John M. Michel, David Mimoun, Anupam Misra, Gilles Montagnac, Franck Montmessin, Valerie Mousset, Naomi Murdoch, Horton Newsom, Logan A. Ott, Zachary R. Ousnamer, Laurent Pares, Yann Parot, Rafal Pawluczyk, C. Glen Peterson, Paolo Pilleri, Patrick Pinet, Gabriel Pont, Francois Poulet, Cheryl Provost, Benjamin Quertier, Heather Quinn, William Rapin, Jean-Michel Reess, Amy H. Regan, Adriana L. Reyes-Newell, Philip J. Romano, Clement Royer, Fernando Rull, Benigno Sandoval, Joseph H. Sarrao, Violaine Sautter, Marcel J. Schoppers, Susanne Schröder, Daniel Seitz, Terra Shepherd, Pablo Sobron, Bruno Dubois, Vishnu Sridhar, Michael J. Toplis, Imanol Torre-Fdez, Ian A. Trettel, Mark Underwood, Andres Valdez, Jacob Valdez, Dawn Venhaus, and Peter Willis

Published
2020
Full Text
View/download PDF

4. Atomic Layer Deposition of Bioactive TiO2 Thin Films on Polyetheretherketone for Orthopedic Implants

Author

Monika Fleischer, Daniel Seitz, Volker Bucher, Andreas Ottenschläger, and Felix Blendinger

Subjects
Contact angle, Atomic layer deposition, Materials science, Coating, Ultimate tensile strength, Surface roughness, engineering, Peek, General Materials Science, engineering.material, Composite material, Thin film, Osseointegration
Abstract

TiO2 thin films were deposited on the orthopedic implant material polyetheretherketone (PEEK) by plasma enhanced atomic layer deposition (PEALD) and characterized for their ability to enhance the osseointegrative properties. PEALD was chosen for film deposition to circumvent drawbacks present in line-of-sight deposition techniques, which require technically complex setups for a homogeneous coating thickness. Film conformality was analyzed on silicon 3D test structures and PEEK with micron-scale surface roughness. Wettability and surface energy were determined through contact angle measurements; film roughness and crystallinity were determined by atomic force microscopy and X-ray diffraction, respectively. Adhesion properties of TiO2 on PEEK were determined with tensile strength tests. Cell tests were performed with the mouse mesenchymal tumor stem cell line ST-2. TiO2-coated PEEK disks were used as substrates for cell proliferation tests and long-term differentiation tests. After 28 days of cultivation, a mineralized bone matrix was observed. Furthermore, the collagen I and osteocalcin content were determined. The results reveal that the osteogenic properties of the TiO2 thin film are comparable to those of hydroxyapatite, and thus bioactive properties of PEEK implants are improved by TiO2 thin films deposited with PEALD.

Published
2021
Full Text
View/download PDF

6. Osteogenic Differentiation of Human Adipose-Derived Stem Cells Seeded on a Biomimetic Spongiosa-like Scaffold: Bone Morphogenetic Protein-2 Delivery by Overexpressing Fascia

Author

Bin Ren, Oliver B. Betz, Daniel Seitz, Christian Thirion, Michael Salomon, Volkmar Jansson, Peter E. Müller, and Volker M. Betz

Subjects
Stem Cells, Organic Chemistry, Bone Morphogenetic Protein 2, Cell Differentiation, General Medicine, adipose-derived stem cells, fascia, osteogenic differentiation, delivery system, scaffold, Catalysis, Computer Science Applications, Inorganic Chemistry, Adipose Tissue, Biomimetics, Osteogenesis, Humans, Intercellular Signaling Peptides and Proteins, Physical and Theoretical Chemistry, Fascia, Molecular Biology, Spectroscopy, Cells, Cultured
Abstract

Human adipose-derived stem cells (hADSCs) have the capacity for osteogenic differentiation and, in combination with suitable biomaterials and growth factors, the regeneration of bone defects. In order to differentiate hADSCs into the osteogenic lineage, bone morphogenetic proteins (BMPs) have been proven to be highly effective, especially when expressed locally by route of gene transfer, providing a constant stimulus over an extended period of time. However, the creation of genetically modified hADSCs is laborious and time-consuming, which hinders clinical translation of the approach. Instead, expedited single-surgery gene therapy strategies must be developed. Therefore, in an in vitro experiment, we evaluated a novel growth factor delivery system, comprising adenoviral BMP-2 transduced fascia tissue in terms of BMP-2 release kinetics and osteogenic effects, on hADSCs seeded on an innovative biomimetic spongiosa-like scaffold. As compared to direct BMP-2 transduction of hADSCs or addition of recombinant BMP-2, overexpressing fascia provided a more uniform, constant level of BMP-2 over 30 days. Despite considerably higher BMP-2 peak levels in the comparison groups, delivery by overexpressing fascia led to a strong osteogenic response of hADSCs. The use of BMP-2 transduced fascia in combination with hADSCs may evolve into an expedited single-surgery gene transfer approach to bone repair.

Published
2022
Full Text
View/download PDF

7. The SuperCam Instrument Suite on the NASA Mars 2020 Rover: Body Unit and Combined System Tests

Author

Francois Poulet, Nina Lanza, John Michel, Kerry Boyd, Valerie Mousset, Fernando Rull, Anupam K. Misra, Horton E. Newsom, Magdalena Dale, Richard Leveille, Sylvain Bernard, Karim Benzerara, Logan Ott, Timothy H. McConnochie, M. George Duran, Jonathan Deming, C. Glen Peterson, Jorden Celis, Juan Manuel Madariaga, Anthony Nelson, Elizabeth C. Auden, Violaine Sautter, Paolo Pilleri, Naomi Murdoch, Susanne Schröder, Joseph H. Sarrao, Miles Egan, Bruno Dubois, Ann Ollila, Roberta A. Klisiewicz, M. Deleuze, K. McCabe, Ryan B. Anderson, Kevin Clark, Noureddine Melikechi, Jens Frydenvang, Matthew R. Dirmyer, A. Regan, Pierre Beck, Olivier Forni, A. Reyes-Newell, David Mimoun, Lauren DeFlores, Stéphane Le Mouélic, Nicolas Mangold, Eric Lorigny, Denine Gasway, John P. Grotzinger, M. Caffrey, Shiv K. Sharma, J. Javier Laserna, Olivier Gasnault, Steven P. Love, Eric Lewin, Sophie Jacquinod, Jeffrey R. Johnson, Dorothea Delapp, Soren N. Madsen, James Lake, Kepa Castro, Joan Ervin, Olivier Beyssac, C. Donny, Yann Parot, J. P. Martinez, Pierre-Yves Meslin, Gabriel Pont, Jean-Michel Reess, L. Parès, P. Bernardi, D. Venhaus, Guillermo Lopez-Reyes, Benjamin Quertier, Gorka Arana, Morten Madsen, Ivair Gontijo, Ralph D. Lorenz, Philip J. Romano, Ian A. Trettel, S. Michael Angel, Gilles Montagnac, Joseph Becker, Vishnu Sridhar, Rafal Pawluczyk, Jérémie Lasue, P. Cais, William Rapin, Jose Antonio Manrique, Xavier Jacob, Clement Royer, Jacob Valdez, I. Torre-Fdez, Amaury Fau, Peter Willis, Louis Borges, Cheryl Provost, Elizabeth C. Cordoba, M. L. Underwood, Justin McGlown, Daniel Seitz, S. A. Storms, Briana Lucero, Heather Quinn, Thierry Fouchet, Raymond Newell, Cécile Fabre, B. Chide, Y. André, Jeffrey Carlson, Roger C. Wiens, Scott M. McLennan, Woodward W. Fischer, Benigno Sandoval, S. Robinson, Patrick Pinet, Samuel M. Clegg, Agnes Cousin, Sylvestre Maurice, Edward A. Cloutis, Gilles Dromart, Franck Montmessin, C. Legett, Andres Valdez, Bruno Bousquet, Reuben Fresquez, Terra Shepherd, Zachary R. Ousnamer, Pablo Sobron, M. Toplis, Marcel J. Schoppers, Jesús Martínez-Frías, D. T. Beckman, Los Alamos National Laboratory (LANL), Institut de recherche en astrophysique et planétologie (IRAP), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Astrophysique de Bordeaux [Pessac] (LAB), Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'études spatiales et d'instrumentation en astrophysique = Laboratory of Space Studies and Instrumentation in Astrophysics (LESIA), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), University of Hawai‘i [Mānoa] (UHM), Astrogeology Science Center [Flagstaff], United States Geological Survey [Reston] (USGS), Centre National d'Études Spatiales [Toulouse] (CNES), University of South Carolina [Columbia], Universidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne] (UPV/EHU), Institut de Planétologie et d'Astrophysique de Grenoble (IPAG), Centre National d'Études Spatiales [Toulouse] (CNES)-Observatoire des Sciences de l'Univers de Grenoble (OSUG ), Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Météo-France -Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Météo-France, Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), Muséum national d'Histoire naturelle (MNHN)-Institut de recherche pour le développement [IRD] : UR206-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre d'Etudes Lasers Intenses et Applications (CELIA), Université de Bordeaux (UB)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Jet Propulsion Laboratory (JPL), NASA-California Institute of Technology (CALTECH), Institut Supérieur de l'Aéronautique et de l'Espace (ISAE-SUPAERO), University of Winnipeg, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), GeoRessources, Institut national des sciences de l'Univers (INSU - CNRS)-Centre de recherches sur la géologie des matières premières minérales et énergétiques (CREGU)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), California Institute of Technology (CALTECH), University of Copenhagen = Københavns Universitet (UCPH), Laboratoire de Planétologie et Géodynamique [UMR 6112] (LPG), Université d'Angers (UA)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de mécanique des fluides de Toulouse (IMFT), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Johns Hopkins University Applied Physics Laboratory [Laurel, MD] (APL), Universidad de Valladolid [Valladolid] (UVa), Universidad de Málaga [Málaga] = University of Málaga [Málaga], McGill University = Université McGill [Montréal, Canada], Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), University of Maryland [College Park], University of Maryland System, State University of New York (SUNY), University of Massachusetts [Lowell] (UMass Lowell), University of Massachusetts System (UMASS), PLANETO - LATMOS, Laboratoire Atmosphères, Milieux, Observations Spatiales (LATMOS), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), The University of New Mexico [Albuquerque], Institut d'astrophysique spatiale (IAS), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Centre National d’Études Spatiales [Paris] (CNES), FiberTech Optica (FTO), In­sti­tut für Op­ti­sche Sen­sor­sys­te­me, Deutsches Zentrum für Luft- und Raumfahrt [Berlin] (DLR), SETI Institute, Observatoire Midi-Pyrénées (OMP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France, Institut national des sciences de l'Univers (INSU - CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'études spatiales et d'instrumentation en astrophysique (LESIA (UMR_8109)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Bordeaux (UB), Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL)-Centre de recherches sur la géologie des matières premières minérales et énergétiques (CREGU)-Institut national des sciences de l'Univers (INSU - CNRS), University of Copenhagen = Københavns Universitet (KU), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Laboratoire de Planétologie et Géodynamique - Angers (LPG-ANGERS), Université de Nantes (UN)-Université de Nantes (UN)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Sorbonne Université (SU)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Institut Supérieur de l'Aéronautique et de l'Espace - ISAE-SUPAERO (FRANCE), and Centre National de la Recherche Scientifique (CNRS)

Subjects
010504 meteorology & atmospheric sciences, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, Mars, 01 natural sciences, 7. Clean energy, Article, law.invention, Telescope, symbols.namesake, Jezero crater, Optics, ChemCam instrument, law, Microphone on Mars, 0103 physical sciences, SuperCam, planetary exploration, luminescence, Traitement du signal et de l'image, Perseverance rover, Spectroscopy, 010303 astronomy & astrophysics, Infrared spectroscopy, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Physics, laboratory curiosity rover, remote Raman system, LIBS, Spectrometer, business.industry, Detector, Astronomy and Astrophysics, Mars Exploration Program, Gale crater, Laser, induced breakdown spectroscopy, Wavelength, in-situ, mission, 13. Climate action, Space and Planetary Science, [SDU]Sciences of the Universe [physics], Raman spectroscopy, symbols, business
Abstract

The SuperCam instrument suite provides the Mars 2020 rover, Perseverance, with a number of versatile remote-sensing techniques that can be used at long distance as well as within the robotic-arm workspace. These include laser-induced breakdown spectroscopy (LIBS), remote time-resolved Raman and luminescence spectroscopies, and visible and infrared (VISIR; separately referred to as VIS and IR) reflectance spectroscopy. A remote micro-imager (RMI) provides high-resolution color context imaging, and a microphone can be used as a stand-alone tool for environmental studies or to determine physical properties of rocks and soils from shock waves of laser-produced plasmas. SuperCam is built in three parts: The mast unit (MU), consisting of the laser, telescope, RMI, IR spectrometer, and associated electronics, is described in a companion paper. The on-board calibration targets are described in another companion paper. Here we describe SuperCam's body unit (BU) and testing of the integrated instrument. The BU, mounted inside the rover body, receives light from the MU via a 5.8 m optical fiber. The light is split into three wavelength bands by a demultiplexer, and is routed via fiber bundles to three optical spectrometers, two of which (UV and violet; 245-340 and 385-465 nm) are crossed Czerny-Turner reflection spectrometers, nearly identical to their counterparts on ChemCam. The third is a high-efficiency transmission spectrometer containing an optical intensifier capable of gating exposures to 100 ns or longer, with variable delay times relative to the laser pulse. This spectrometer covers 535-853 nm ( 105 - 7070 cm − 1 Raman shift relative to the 532 nm green laser beam) with 12 cm − 1 full-width at half-maximum peak resolution in the Raman fingerprint region. The BU electronics boards interface with the rover and control the instrument, returning data to the rover. Thermal systems maintain a warm temperature during cruise to Mars to avoid contamination on the optics, and cool the detectors during operations on Mars. Results obtained with the integrated instrument demonstrate its capabilities for LIBS, for which a library of 332 standards was developed. Examples of Raman and VISIR spectroscopy are shown, demonstrating clear mineral identification with both techniques. Luminescence spectra demonstrate the utility of having both spectral and temporal dimensions. Finally, RMI and microphone tests on the rover demonstrate the capabilities of these subsystems as well., Proyecto MINECO Retos de la Sociedad. Ref. ESP2017-87690-C3-1-R

Published
2021
Full Text
View/download PDF

10. S1321 Targeting Quality Measures in Cirrhosis: A Guidelines-Based Initiative to Improve Inpatient Cirrhosis Management

Author

Karalea Mulvey, Steven Gerke, Daniel Seitz, Eric S. Orman, Katherine Merrill, Indira Bhavsar-Burke, Nabil Fayad, Sean M. Burke, and Nour Hamade

Subjects
medicine.medical_specialty, Cirrhosis, Hepatology, business.industry, media_common.quotation_subject, Gastroenterology, medicine, Quality (business), Intensive care medicine, business, medicine.disease, media_common
Published
2021
Full Text
View/download PDF

11. Induction of Articular Chondrogenesis by Chitosan/Hyaluronic-Acid-Based Biomimetic Matrices Using Human Adipose-Derived Stem Cells

Author

Volkmar Jansson, Roland M. Klar, Daniel Seitz, Fabian König, Peter E. Müller, and Yijiang Huang

Subjects
Cartilage, Articular, 0301 basic medicine, Cellular differentiation, 02 engineering and technology, Regenerative medicine, lcsh:Chemistry, chemistry.chemical_compound, Tissue engineering, Biomimetic Materials, Hyaluronic acid, hyaluronic acid, lcsh:QH301-705.5, Spectroscopy, hTGF-β3, Tissue Scaffolds, Hyaline cartilage, Stem Cells, biomaterial, Cell Differentiation, General Medicine, Computer Science Applications, Cell biology, medicine.anatomical_structure, Adipose Tissue, articular chondrogenesis, hBMP-6, Stem cell, Chondrogenesis, Cell Survival, 0206 medical engineering, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, medicine, Humans, Physical and Theoretical Chemistry, Molecular Biology, Cell Proliferation, Cartilage, Organic Chemistry, 020601 biomedical engineering, 030104 developmental biology, Gene Expression Regulation, chemistry, lcsh:Biology (General), lcsh:QD1-999, adipose-derived stem cells, chitosan
Abstract

Cartilage repair using tissue engineering is the most advanced clinical application in regenerative medicine, yet available solutions remain unsuccessful in reconstructing native cartilage in its proprietary form and function. Previous investigations have suggested that the combination of specific bioactive elements combined with a natural polymer could generate carrier matrices that enhance activities of seeded stem cells and possibly induce the desired matrix formation. The present study sought to clarify this by assessing whether a chitosan-hyaluronic-acid-based biomimetic matrix in conjunction with adipose-derived stem cells could support articular hyaline cartilage formation in relation to a standard chitosan-based construct. By assessing cellular development, matrix formation, and key gene/protein expressions during in vitro cultivation utilizing quantitative gene and immunofluorescent assays, results showed that chitosan with hyaluronic acid provides a suitable environment that supports stem cell differentiation towards cartilage matrix producing chondrocytes. However, on the molecular gene expression level, it has become apparent that, without combinations of morphogens, in the chondrogenic medium, hyaluronic acid with chitosan has a very limited capacity to stimulate and maintain stem cells in an articular chondrogenic state, suggesting that cocktails of various growth factors are one of the key features to regenerate articular cartilage, clinically.

Published
2019

12. Digital Supply Chains : A Practitioner's Guide to Successful Digitalization

Author

Thomas Mrozek, Daniel Seitz, Kai-Uwe Gundermann, Matthias Dicke, Thomas Mrozek, Daniel Seitz, Kai-Uwe Gundermann, and Matthias Dicke

Subjects
Materials management--Data processing, Inventory control--Data processing, Business logistics--Information technology
Abstract

Concrete instructions for digital transformation in business Supply Chain Management is without question deeply affected by the disruptive forces of a modern organization, positively as well as negatively. Between Advanced Analytics and AI, agile role models and autonomous warehouses a senior executive is often in danger of losing track in the digital jungle. In these exciting times, with their many turning points, h&z aims to share insights around digital supply chains, their application in business and the so vital transformation to successfully prepare organizations for this challenge. This book provides insights into best practices current supply chain and how CSOs apply technologies and advancements. It also makes daring forecasts about how processes and leadership must be designed so that the digital transformation does not fail in its infancy, but leads to a truly agile organization.

Published
2020

13. Glucosamine Hydrochloride and N-Acetylglucosamine Influence the Response of Bovine Chondrocytes to TGF-β3 and IGF in Monolayer and Three-Dimensional Tissue Culture

Author

Daniel Seitz, Carlos Rodrigo de Mello Roesler, André Luiz Almeida Pizzolatti, Gean V. Salmoria, and Florian Gaudig

Subjects
030203 arthritis & rheumatology, 0301 basic medicine, biology, Cell growth, Biomedical Engineering, Medicine (miscellaneous), Molecular biology, Chitosan, carbohydrates (lipids), 03 medical and health sciences, chemistry.chemical_compound, Tissue culture, 030104 developmental biology, 0302 clinical medicine, chemistry, Proteoglycan, Glucosamine, biology.protein, N-Acetylglucosamine, lipids (amino acids, peptides, and proteins), Original Article, Stem cell, Transforming growth factor
Abstract

BACKGROUND: Glucosamine hydrochloride (GlcN·HCl) has been shown to inhibit cell growth and matrix synthesis, but not with N-acetyl-glucosamine (GlcNAc) supplementation. This effect might be related to an inhibition of critical growth factors (GF), or to a different metabolization of the two glucosamine derivatives. The aim of the present study was to evaluate the synergy between GlcN·HCl, GlcNAc, and GF on proliferation and cartilage matrix synthesis. METHOD: Bovine chondrocytes were cultivated in monolayers for 48 h and in three-dimensional (3D) chitosan scaffolds for 30 days in perfusion bioreactors. Serum-free (SF) medium was supplemented with either growth factors (GF) TGF-β (5 ng mL(−1)) and IGF-I (10 ng mL(−1)), GlcN·HCl or GlcNAc at 1mM each or both. Six groups were compared according to medium supplementation: (a) SF control; (b) SF + GlcN·HCl; (c) SF + GlcNAc; (d) SF + GF; (e) SF + GF + GlcN·HCl; and (f) SF + GF + GlcNAc. Cell proliferation, proteoglycan, collagen I (COL1), and collagen II (COL2) synthesis were evaluated. RESULTS: The two glucosamines showed opposite effects in monolayer culture: GlcN·HCl significantly reduced proliferation and GlcNAc significantly augmented cellular metabolism. In the 30 days 3D culture, the GlcN·HCl added to GF stimulated cell proliferation more than when compared to GF only, but the proteoglycan synthesis was smaller than GF. However, GlcNAc added to GF improved the cell proliferation and proteoglycan synthesis more than when compared to GF and GF/GlcN·HCl. The synthesis of COL1 and COL2 was observed in all groups containing GF. CONCLUSION: GlcN·HCl and GlcNAc increased cell growth and stimulated COL2 synthesis in long-time 3D culture. However, only GlcNAc added to GF improved proteoglycan synthesis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13770-018-0150-x) contains supplementary material, which is available to authorized users.

Published
2018

14. 相対情報に基づく3次元群れ制御

Author

Tatsuya Ibuki, Masayuki Fujita, Takeshi Hatanaka, and Daniel Seitz

Subjects
Nonlinear Sciences::Adaptation and Self-Organizing Systems, Rigid Body Motion, Cooperative Control, Flocking, Synchronization, Quantitative Biology::Other, GeneralLiterature_MISCELLANEOUS
Abstract

This paper proposes a flocking algorithm on the Special Euclidean group SE(3) for a network of rigid bodies. The main feature of the present law is to embody the three fundamental flocking rules, cohesion, alignment, and separation, presented by Reynolds. We first define rigid body networks and tackle a pose synchronization problem as a partial flocking problem. Then, based on the Reynolds rules, we define flocking motion as the goal of this paper, propose a flocking algorithm and give the convergence analysis. Here, the present scheme is based only on relative pose information to neighbors for imitating actual flocking motion or facility of implementation. We finally show the effectiveness of the present algorithm through simulation.

Published
2015

16. An Overview of Regulatory Issues for Yoga, Yoga Therapy, and Ayurveda

Author

Daniel Seitz

Subjects
Licensure, medicine.medical_specialty, business.industry, Alternative medicine, General Medicine, Field (computer science), Variety (cybernetics), Politics, Nursing, Credibility, Health care, medicine, Engineering ethics, Set (psychology), business
Abstract

In order to gain greater credibility, emerging healthcare and health-related fields usually establish a variety of self-regulatory structures and organizations. These structures serve to promote safe and effective practice, strengthen the field's legal status, expand professional opportunities, increase the profession's political influence, and legitimize a field in the eyes of potential patients, potential students, governmental entities, and the healthcare industry. Self-regulatory structures can also set the groundwork for professional licensure and other types of external recognition. Developing self-regulatory structures, however, can pose significant challenges and invariably involve trade-offs. Therefore, practitioners and educators within emerging fields should engage in inclusive, representational, and transparent decision-making processes to build support for any self-regulatory measures being considered.

Published
2010
Full Text
View/download PDF

17. Megawatt-level peak-power from a passively Q-switched hybrid fiber-bulk amplifier and its applications

Author

Peter Genter, Juraj Bdzoch, Daniel Seitz, Axel Reiser, Sven Höfer, Nicolas Kugler, and Sina Scholz-Riecke

Subjects
Materials science, business.industry, Amplifier, Single-mode optical fiber, Pulse duration, 02 engineering and technology, 021001 nanoscience & nanotechnology, Laser, 01 natural sciences, Q-switching, law.invention, 010309 optics, Optics, Mode-locking, law, 0103 physical sciences, Laser power scaling, Laser beam quality, 0210 nano-technology, business
Abstract

A novel laser system with optical parameters that fill the gap between Q-switched and modelocked lasers has been developed. It consists of a high gain hybrid fiber-bulk amplifier seeded by a low power SESAM Q-switched oscillator. The mW level output power of the seed oscillator is preamplified by a single mode fiber which is limited by SRS effects. The final amplification stage is realized by a longitudinal pumped Nd:YVO4 crystal in a double pass setup. This MOPA configuration delivers sub-300ps pulses at repetition rates up to 1 MHz with an output power exceeding 60W. Nonlinear frequency conversion to 532nm and 355nm is achieved with efficiencies of >75% and >45%, respectively. Due to the high peak power, high repetition rate and high beam quality of this system, applications formerly only addressable at lower pulse repetition frequencies or with complex modelocked laser systems are now possible with high speed and lower cost of ownership. Application results that take benefit from these new laser parameters will be shown. Furthermore, the reduction of the pulse duration to sub-100ps and power scaling to output powers

Published
2016
Full Text
View/download PDF

18. Is Hydroxyapatite Ceramic Included in the Bone Remodelling Proccess? An In Vitro Study of Resorption and Formation Processes

Author

Daniel Seitz, Günter Ziegler, Helmar Mayr, and Rainer Detsch

Subjects
Materials science, Stromal cell, Osteoid, Mechanical Engineering, Bone healing, Bone resorption, Bone remodeling, Cell biology, Resorption, medicine.anatomical_structure, Mechanics of Materials, Bone cell, medicine, General Materials Science, Bone marrow, Biomedical engineering
Abstract

Bone remodelling is a coupled process of bone formation and resorption. This process is physiologically controlled and involves the synthesis of bone matrix by osteoblasts and bone resorption by giant cells called osteoclasts. It includes a complex interaction of cells, with specific chemokine signalling. In order to study bone remodelling in vitro, we cultivated two precursor cellline types in a model coculture system on synthetic hydroxyapatite ceramic (HA) for 14 days. The monocytes have the capacity to differentiate into osteoclast-like cells, and the bone marrow stromal cells can differentiate into osteoblast-like cells. This coculture was used to analyse the in vitro cell interaction between monocytes and stromal cells. Furthermore, the attachment of the bone marrow stromal cells to the resorbed HA-surfaces was studied. In this in vitro study we demonstrated osteoclast-like differentiation and bone marrow stromal growth in a coculture system on a synthetic bone substitute material. Under optimal conditions, HA can be resorbed and bone marrow cells can grow into the lacunas to form new bone. These results give important cues for the adjustment of synthetic bone substitute materials for optimal remodelling behaviour.

Published
2007
Full Text
View/download PDF

19. Partizipation und Engagement im Netz : Neue Chancen für Demokratie und Medienpädagogik

Author

Klaus Lutz, Eike Rösch, Daniel Seitz, Klaus Lutz, Eike Rösch, and Daniel Seitz

Abstract

Das Internet bietet eine Vielzahl an Gelegenheiten von politischer Beteiligung für Heranwachsende. So kann z.B. ein Klick auf den'Gefällt mir'-Button in Sozialen Netzwerken nur eine flüchtige Stellungnahme sein, aber auch Ausdruck einer wohldurchdachten politischen Äußerung. Um alle Jugendlichen an diesen Möglichkeiten teilhaben zu lassen, ist Medienbildung von zentraler Bedeutung. Jugendliche dabei anzuleiten, die neuen medialen Möglichkeiten im Sinne ihrer Interessen zu nutzen, ist eine der großen Herausforderungen für die Medienpädagogik der nächsten Jahre.Wie können die (jungen) Bürgerinnen und Bürger überhaupt motiviert werden, sich an gesellschaftlichen Diskursen zu beteiligen und welchen Einfluss hat eine solche zivilgesellschaftliche Beteiligung? Wo existieren gesellschaftliche Hindernisse für gelingende Beteiligung? Wie lassen sich diese ausräumen?Dieser Band bietet einen Überblick über die momentane ePartizipationsdebatte, gibt aber auch gleichzeitig Antworten auf einige der pädagogischen Fragestellungen, die sich aus dieser Debatte ableiten. Hierbei stehen der theoretische Diskurs und die gewonnenen Erkenntnisse aus der Praxis gleichberechtigt nebeneinander.Die Pädagogik ist aufgerufen, Partizipationsprozesse stärker als bisher in der Medienpädagogik zu verankern, Jugendlichen etwas zuzutrauen und ihnen Rahmenbedingungen zur Verfügung zu stellen, die ePartizipation ermöglichen - jenseits von Sucht- und Demenzdebatten oder gerade deshalb. Dazu möchte der vorliegende Band beitragen.

Published
2013

Catalog

Books, media, physical & digital resources
See catalog results