Search

Your search keyword '"Daniel Ouro"' showing total 2 results
Start Over Author "Daniel Ouro"
2 results on '"Daniel Ouro"'

1. Maternal caloric restriction implemented during the preconceptional and pregnancy period alters hypothalamic and hippocampal endocannabinoid levels at birth and induces overweight and increased adiposity at adulthood in male rat offspring

Author

MARÍA TERESA RAMÍREZ-LÓPEZ, Mariam Vazquez, Laura Bindila, Ermelinda Lomazzo, Clementine Hofmann, Noemi Blanco, Francisco Alen, MARIA ANTÓN, Juan Decara, Rocío Arco, Daniel Ouro, LAURA ORIO, JUAN SUAREZ, Beat Lutz, Raquel Gomez de Heras, and FERNANDO RODRIGUEZ DE FONSECA

Subjects
Behavior, Endocannabinoids, Hippocampus, Hypothalamus, Metabolism, Olfactory Bulb, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

Exposure to inadequate nutritional conditions in critical windows of development has been associated to disturbances on metabolism and behavior in the offspring later in life. The role of the endocannabinoid system, a known regulator of energy expenditure and adaptive behaviors, in the modulation of these processes is unknown. In the present study, we investigated the impact of exposing rat dams to diet restriction (20% less calories than standard diet) during pre-gestational and gestational periods on a) neonatal outcomes, b) endocannabinoid content in hypothalamus, hippocampus and olfactory bulb at birth, c) metabolism-related parameters, and d) behavior in adult male offspring. We found that calorie-restricted dams tended to have a reduced litter size, although the offspring showed normal weight at birth. Pups from calorie-restricted dams also exhibited a strong decrease in the levels of anandamide (AEA), 2-arachidonoylglycerol (2-AG), arachidonic acid (AA) and palmitoylethanolamide (PEA) in the hypothalamus at birth. Additionally, pups from diet-restricted dams displayed reduced levels of AEA in the hippocampus without significant differences in the olfactory bulb. Moreover, offspring exhibited increased weight gain, body weight and adiposity in adulthood as well as increased anxiety-related responses. We propose that endocannabinoid signaling is altered by a maternal caloric restriction implemented during the preconceptional and pregnancy periods, which might lead to modifications of the hypothalamic and hippocampal circuits, potentially contributing to the long-term effects found in the adult offspring.

Published
2016
Full Text
View/download PDF

2. Exposure to a Highly Caloric Palatable Diet During Pregestational and Gestational Periods Affects Hypothalamic and Hippocampal Endocannabinoid Levels at Birth and Induces Adiposity and Anxiety-Like Behaviors in Male Rat Offspring

Author

Juan Decara, Ermelinda Lomazzo, Laura Bindila, Laura Orio, Clementine Hofmann, Daniel Ouro, Rosario Noemi Blanco, Juan Suárez, Francisco Alén, María Teresa Ramírez-López, Fernando Rodríguez de Fonseca, Raquel Gómez de Heras, Beat Lutz, María Antón, Mariam Vázquez, [Ramírez-López,MT, Vázquez,M, Blanco,RN, Alén,F, Antón,M, Ouro,L, Rodríguez de Fonseca,F, Gómez de Heras,R] Departamento de Psicobiología, Facultad de Psicología, Universidad Complutense de Madrid, Madrid, Spain. [Vázquez,M, Degara,J, Suarez,J, Rodríguez de Fonseca,F] Unidad de Gestión Clínica de Salud Mental, Instituto IBIMA, Hospital Regional Universitario de Málaga, Universidad de Málaga, Málaga, Spain. [Bindila,L, Lomazzo,E, Hofmann,C, Lutz,B] Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany., This work was supported by the Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (PI13/0226 to FR, CP12/03109 to JS and PSI-2012-35388 to RG), Red de Trastornos Adictivos (RD12/0028/0001 to FR), CIBERobn, Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, UE/ERDF (PI45403, CTS-8221, CTS-433 to FR), Consejería de Salud, Junta de Andalucía (PI0232/2008, PI0029/2008, and SAS111224 to JS and FR), and the German Research Foundation DFG (FOR926, project CP1 to BL). MT RL has been funded by a FPU predoctoral fellowship of the Spanish Ministerio de Educación, Cultura y Deporte (AP-2009-0225), and JS holds 'Miguel Servet' research contract from the National System of Health, ISCIII (grant numbers CP14/00173).

Subjects
0301 basic medicine, Phenomena and Processes::Genetic Phenomena::Phenotype [Medical Subject Headings], hippocampus, Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Postpartum Period::Lactation [Medical Subject Headings], Anxiety, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Body Constitution::Body Weights and Measures::Body Fat Distribution::Adiposity [Medical Subject Headings], Hippocampus, Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Feeding Behavior::Food Preferences [Medical Subject Headings], Open field, Behavioral Neuroscience, Oleoylethanolamide, chemistry.chemical_compound, Named Groups::Persons::Parents::Mothers [Medical Subject Headings], 0302 clinical medicine, Lactation, Health Care::Environment and Public Health::Public Health::Environmental Pollution::Environmental Exposure::Maternal Exposure [Medical Subject Headings], maternal diet, hypothalamus, Masculino, Original Research, Phenomena and Processes::Physiological Phenomena::Physiological Processes::Nutrition Processes::Weaning [Medical Subject Headings], Adipogenesis, Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons, Acyclic::Alkenes::Polyunsaturated Alkamides [Medical Subject Headings], Anandamide, anxiety, Endocannabinoid system, Etanolaminas, Endocannabinoides, Exposición materna, Alcamidas poliinsaturadas, Neuropsychology and Physiological Psychology, medicine.anatomical_structure, Adiposidad, Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids, Unsaturated::Fatty Acids, Monounsaturated::Oleic Acids [Medical Subject Headings], Cannabinoides, Emociones, Gestation, Dieta, Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Hormones, Hormone Substitutes, and Hormone Antagonists::Hormones::Endocannabinoids [Medical Subject Headings], Fenotipo, Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Terpenes::Cannabinoids [Medical Subject Headings], Chemicals and Drugs::Lipids::Fatty Acids::Palmitic Acids [Medical Subject Headings], Chemicals and Drugs::Lipids::Fatty Acids::Fatty Acids, Unsaturated::Arachidonic Acids [Medical Subject Headings], medicine.medical_specialty, Offspring, Cognitive Neuroscience, Preferencias alimentarias, Hypothalamus, Check Tags::Male [Medical Subject Headings], Development, Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet [Medical Subject Headings], Biology, lcsh:RC321-571, adipogenesis, Ácidos oléicos, Ansiedad, Ratas, 03 medical and health sciences, Internal medicine, Hipocampo, medicine, Maternal diet, Anatomy::Nervous System::Central Nervous System::Brain::Limbic System::Hippocampus [Medical Subject Headings], endocannabinoids, Psychiatry and Psychology::Behavior and Behavior Mechanisms::Emotions::Anxiety [Medical Subject Headings], lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, development, Ácido araquidónico, Cacao, Palmitoylethanolamide, Lactancia, Chemicals and Drugs::Organic Chemicals::Amines::Amino Alcohols::Ethanolamines [Medical Subject Headings], Chemicals and Drugs::Lipids::Glycerides [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Rats [Medical Subject Headings], 030104 developmental biology, Endocrinology, chemistry, Destete, Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Sterculiaceae::Cacao [Medical Subject Headings], Psychiatry and Psychology::Behavior and Behavior Mechanisms::Emotions [Medical Subject Headings], Madres, Glicéridos, Ácidos palmíticos, 030217 neurology & neurosurgery, Endocannabinoids, Neuroscience
Abstract

Journal Article; Exposure to unbalanced diets during pre-gestational and gestational periods may result in long-term alterations in metabolism and behavior. The contribution of the endocannabinoid system to these long-term adaptive responses is unknown. In the present study, we investigated the impact of female rat exposure to a hypercaloric-hypoproteic palatable diet during pre-gestational, gestational and lactational periods on the development of male offspring. In addition, the hypothalamic and hippocampal endocannabinoid contents at birth and the behavioral performance in adulthood were investigated. Exposure to a palatable diet resulted in low weight offspring who exhibited low hypothalamic contents of arachidonic acid and the two major endocannabinoids (anandamide and 2-arachidonoylglycerol) at birth. Palmitoylethanolamide, but not oleoylethanolamide, also decreased. Additionally, pups from palatable diet-fed dams displayed lower levels of anandamide and palmitoylethanolamide in the hippocampus. The low-weight male offspring, born from palatable diet exposed mothers, gained less weight during lactation and although they recovered weight during the post-weaning period, they developed abdominal adiposity in adulthood. These animals exhibited anxiety-like behavior in the elevated plus-maze and open field test and a low preference for a chocolate diet in a food preference test, indicating that maternal exposure to a hypercaloric diet induces long-term behavioral alterations in male offspring. These results suggest that maternal diet alterations in the function of the endogenous cannabinoid system can mediate the observed phenotype of the offspring, since both hypothalamic and hippocampal endocannabinoids regulate feeding, metabolic adaptions to caloric diets, learning, memory, and emotions. Yes

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results