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Facial paralysis can result in severe implications for the patients. However, stem cell biology has become an important field in regenerative medicine since the discovery and characterization of mesenchymal stem cells. Our aim was to evaluate the regeneration after facial nerve crush injury and application of human immature dental pulp stem cells (iDPSC). For this study 70 Wistar rats underwent a unilateral facial nerve crush injury and were divided into two groups: Group I (GI): Crushed; Group II (GII): Crushed and iDPSC, and distributed into study periods of 3, 7, 14, 21, and 42 postoperative days. Facial nerve regeneration was analyzed via functional recovery of whisker movement, histomorphometric analysis, and immunoblotting assay. The results show that GII had complete functional recovery at 14 days, while GI recovered after 42 days. Also, regarding the facial nerve trunk, GII presented histological improvement, evidencing better axonal and structural organization of the myelin sheath, and exhibited statistically higher values for the outer and inner perimeters and g-ratio. Nevertheless, GI exhibited statistically higher values for the thickness of myelin sheath. In the buccal branch, no differences were observed for all parameters between groups. At 42 days, both groups GI and GII were close to the levels observed for the control group. Concerning nerve growth factor expression, GII exhibited statistically greater values ( p < 0.05) compared with the control group at 7 days. In summary, a single injection of human iDPSC promoted a positive effect on regeneration of the facial nerve trunk after 14 days and provided an alternative to support regeneration following peripheral nerve injury.

Introdução: A Anatomia é fundamental para o desenvolvimento de habilidades médicas e demanda muita atenção e dedicação, principalmente a respeito de origem e inserção muscular. Objetivos: Tornar o estudo dinâmico, atrativo e de fácil memorização por meio do desenvolvimento de MAMD. Métodos: O estudo foi desenvolvido nas aulas práticas de Anatomia do 1º período de Medicina da UNIFAL-MG nas etapas: A) Aula e aplicação de teste de conhecimento 1; B) Elaboração de tabela de origem e inserção; C) Confecção e apresentação dos MAMD; D) Aplicação do teste de conhecimento 2 e do questionário de satisfação; E) Análise dos resultados. Resultados: 65 estudantes divididos em 3 turmas participaram do estudo. Os resultados do 2º teste de conhecimento trouxeram um índice de acertos cerca de 3 vezes maior que os achados no 1º. Os estudantes atribuíram significativa aprovação (89,23%) do método utilizado na aula prática e sugeriram que fosse aplicado para outros segmentos musculares. Conclusão: A confecção dos MAMD estimulou o estudo da anatomia e auxiliou a suprir as demandas por peças anatômicas, visto que é cada vez mais raro o recebimento de cadáveres em laboratórios de anatomia.

Os desenvolvimentos industrial e tecnológico culminaram com a flexibilização da carga horária de trabalho, resultando em alterações biológicas no ciclo circadiano dos indivíduos. Este estudo objetiva analisar se hábitos de vida noturnos relacionados a atividades laborais comprometem a saúde dos trabalhadores. A revisão integrativa foi realizada através de buscas no Portal Regional da Biblioteca Virtual em Saúde (BVS), com a utilização das bases de dados Medline, Lilacs, Ibecs, assim como nas plataformas Pubmed, Google Acadêmico e ResearchGate. Foram incluídos artigos originais de primeira classe, disponíveis na íntegra, detalhados, com publicação entre 2004 a 2021. Foi utilizado um instrumento autoral para a extração dos dados no Excel. Seguiu-se o protocolo da estratégia PICOS. Os resultados obtidos revelaram que noites mal dormidas reduzem o desempenho laboral e aumentam os riscos de acidentes no local de trabalho. O sono favorece o ganho de peso, além de afetar negativamente o humor. Nesse sentido, a qualidade de vida dos trabalhadores do turno da noite é prejudicada, havendo prevalência no consumo de álcool, uso de tabaco, hábitos alimentares inadequados e sedentarismo entre esses indivíduos. Conclui-se que o sono mostra-se importante para a qualidade de vida em todos os aspectos, sejam eles físicos, sociais, emocionais ou cognitivos.

Assess morphologically the efficacy of constant dose (CD) or gradual dose (GD) in photobiomodulation therapy (PBMT) during the regeneration process of rats' mental nerve after compression lesion.Forty-eight male Wistar rats were used and divided into four groups (n = 12): negative control (NC): lesion by compression; positive control (PC): no lesion; GD: lesion by compression and PBMT with GD; and CD: lesion by compression and PBMT with CD. One day after the surgery, the groups GD and CD underwent PBMT daily in three equidistant points around the incision area. The parameters were wavelength of 808 nm, 100 mW, CD received treatment with 120 J/cmIt was observed that PBMT was able to accelerate the process of nerve regeneration presenting an increase in the number of myelinated fibers starting at 14 days of treatment for groups CD and GD, and at 21 days they were similar to PC. It was observed a better lamellar organization of myelin sheath at 7 days for GD and at 14 days for CD, similar to PC. Both GD and CD presented significant differences compared to NC and PC for thickness of the myelin sheath, outer perimeter, internal area, and number of myelin fibers.PBMT presented positive effect on the regeneration of nerve starting at 14 days, and after 21 days there was no difference between GD and CD.

To evaluate the influence of DICOM and JPEG formats on the reproducibility of identification of cephalometric landmarks on posteroanterior digital radiographs. The sample consisted of digital images of posteroanterior radiographs saved in DICOM format; they were subsequently converted into JPEG format with quality factors of 100, 80, and 60. After sample blinding, 18 cephalometric points were identified on each image by three calibrated orthodontists using a system of x- and y-axis Cartesian coordinates. The intraclass correlation test was applied to analyze the occurrence of systematic errors, and Dahlberg’s formula was used to calculate casual errors. The reproducibility between the DICOM and JPEG formats was compared by analysis of variance. All tests were conducted with a significance level of 5 %. Each examiner repeated the procedure 30 days after the initial test with 20 % of the images, which were randomly selected, in each file format. We found that the cephalometric landmarks in the posteroanterior digital radiographs presented intraexaminer and interexaminer reproducibility; the exceptions were points ZL, ZR, AZ, JR, NC, and CN in the y-axis and A6 in the x-axis, which were due to intraclass correlations under 0.90 and casual error over 1.0 mm, irrespective of file format (DICOM or JPEG). The DICOM and JPEG file formats and quality factors of 100, 80, and 60 did not affect the intraexaminer and interexaminer reproducibility of cephalometric landmarks.

Adult stem cells research has been considered the most advanced sort of medical-scientific research, particularly stem cells from human exfoliated deciduous teeth (SHED), which represent an immature stem cell population. The purpose of this review is to describe the current knowledge concerning SHED from full-text scientific publications from 2003 to 2015, available in English language and based on the keyword and/or abbreviations ‘stem cells from human exfoliated deciduous teeth (SHED)', and individually presented as to the properties of SHED, immunomodulatory properties of SHED and stem cell banking. In summary, these cell populations are easily accessible by noninvasive procedures and can be isolated, cultured and expanded in vitro, successfully differentiated in vitro and in vivo into odontoblasts, osteoblasts, chondrocytes, adipocytes and neural cells, and present low immune reactions or rejection following SHED transplantation. Furthermore, SHED are able to remain undifferentiated and stable after long-term cryopreservation. In conclusion, the high proliferative capacity, easy access, multilineage differentiation capacity, noninvasiveness and few ethical concerns make stem cells from human exfoliated deciduous teeth the most valuable source of stem cells for tissue engineering and cell-based regenerative medicine therapies.

Ameloblastoma is a benign locally aggressive infiltrative odontogenic lesion. It is characterized by slow growth and painless swelling. The treatment for ameloblastoma varies from curettage to en bloc resection, and the reported recurrence rates after treatment are high; the safety margin of resection is important to avoid recurrence. Advances in technology brought about great benefits in dentistry; a new generation of computed tomography scanners and 3-dimensional images enhance the surgical planning and management of maxillofacial tumors. The development of new prototyping systems provides accurate 3D biomodels on which surgery can be simulated, especially in cases of ameloblastoma, in which the safety margin is important for treatment success. A case of mandibular follicular ameloblastoma is reported where a 3D biomodel was used before and during surgery.