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Leucine is a critical amino acid stimulating myofibrillar protein synthesis (MyoPS). The consumption of higher leucine-containing drinks stimulates MyoPS, but we know less about higher leucine solid foods. Here, we examined the effect of short-term resistance exercise training (STRT) combined with supplementation of a protein and leucine-enriched bar, compared with STRT alone, on integrated (%/day) rates of MyoPS and anabolic protein signaling. In a nonblinded, randomized crossover trial, eight young adults performed four sessions of STRT without or while consuming the study bar (STRT+Leu, 16 g of protein containing ∼3 g of leucine) for two 4-day phases, separated by 2 days nonexercise (Rest) washout. In combination with serial muscle biopsies, deuterated water permitted the measurement of MyoPS and protein signaling phosphorylation. MyoPS during STRT (1.43 ± 0.06%/day) and STRT+Leu (1.53 ± 0.06%/day) were greater than Rest (1.31 ± 0.05%/day), and MyoPS during STRT+Leu (1.53 ± 0.06%/day) was greater than STRT alone (1.43 ± 0.06%/day). STRT+Leu increased the ratio of phosphorylated to total mechanistic target of rapamycin and 4EBP1 compared to Rest. Engaging in STRT increased integrated MyoPS and protein signaling in young adults and was enhanced with increased protein intake derived from a leucine-enriched protein bar. This study was registered at clinicaltrials.gov as NCT03796897.

We investigated the effects of ingesting a leucine-enriched essential amino acid (EAA) gel alone or combined with resistance exercise (RE) versus RE alone (control) on plasma aminoacidemia and intramyocellular anabolic signaling in healthy younger (28 &plusmn, 4 years) and older (71 &plusmn, 3 years) adults. Blood samples were obtained throughout the three trials, while muscle biopsies were collected in the postabsorptive state and 2 h following RE, following the consumption of two 50 mL EAA gels (40% leucine, 15 g total EAA), and following RE with EAA (combination (COM)). Protein content and the phosphorylation status of key anabolic signaling proteins were determined via immunoblotting. Irrespective of age, during EAA and COM peak leucinemia (younger: 454 &plusmn, 32 &micro, M and 537 &plusmn, 111 &micro, M, older: 417 &plusmn, 99 &micro, M and 553 &plusmn, 136 &micro, M) occurred ~60&ndash, 120 min post-ingestion (younger: 66 &plusmn, 6 min and 120 &plusmn, 60 min, older: 90 &plusmn, 13 min and 78 &plusmn, 12 min). In the pooled sample, the area under the curve for plasma leucine and the sum of branched-chain amino acids was significantly greater in EAA and COM compared with RE. For intramyocellular signaling, significant main effects were found for condition (mTOR (Ser2481), rpS6 (Ser235/236)) and age (S6K1 (Thr421/Ser424), 4E-BP1 (Thr37/46)) in age group analyses. The phosphorylation of rpS6 was of similar magnitude (~8-fold) in pooled and age group data 2 h following COM. Our findings suggest that a gel-based, leucine-enriched EAA supplement is associated with aminoacidemia and a muscle anabolic signaling response, thus representing an effective means of stimulating muscle protein anabolism in younger and older adults following EAA and COM.

The Dietary Reference Intakes set the protein RDA for persons >19 y of age at 0.8 g protein ⋅ kg body weight(−1) ⋅ d(−1). A growing body of evidence suggests, however, that the protein RDA may be inadequate for older individuals. The evidence for recommending a protein intake greater than the RDA comes from a variety of metabolic approaches. Methodologies centered on skeletal muscle are of paramount importance given the age-related decline in skeletal muscle mass and function (sarcopenia) and the degree to which dietary protein could mitigate these declines. In addition to evidence from short-term experimental trials, observational data show that higher protein intakes are associated with greater muscle mass and, more importantly, better muscle function with aging. We are in dire need of more evidence from longer-term intervention trials showing the efficacy of protein intakes that are higher than the RDA in older persons to support skeletal muscle health. We propose that it should be recommended that older individuals consume ≥1.2 g protein · kg(−1) · d(−1) and that there should be an emphasis on the intake of the amino acid leucine, which plays a central role in stimulating skeletal muscle anabolism. Critically, the often-cited potential negative effects of consuming higher protein intakes on renal and bone health are without a scientific foundation in humans.

We examined the aminoacidemic, glycemic, and insulinemic responses following ingestion of 25 g of native whey protein, micellar casein, and a 1:1 blend of whey and casein in randomized order in young adult men. Blood samples were drawn at baseline and at regular intervals for 6 h following ingestion. Area under curve and peak plasma essential amino acid concentrations after the ingestion of the protein blend were similar to whey and greater compared with casein.

The purposes of this study were to: (a) examine the age-related patterns of differences in height (HT), body mass (BM), percent body fat (% fat), body mass index (BMI), and skinfolds (SF) in 11- to 18-year-old wrestlers; (b) determine the coherence of direct (% fat) and indirect (BMI and SFs) indicators of adiposity in the wrestlers; and (c) compare the age-related patterns and mean values for HT, BM, BMI, subscapular, and triceps SF for the wrestlers to those of national samples of boys from the National Health and Nutrition Examination Survey (NHANES) database. One hundred thirty wrestlers were divided into 8 independent yearly age groups (AG): AG11-AG18 years. Height, BM, BMI, subscapular SF, triceps SF, medial calf SF, thigh SF, sum of SFs, and % fat were assessed. There were no differences between the wrestlers and NHANES samples for age-related patterns of BMI (0.61 and 0.63 kg·m·y), subscapular SF (0.47 and 0.37 mm·y), or triceps SF (-0.31 and -0.39 mm·y). Furthermore, the wrestlers displayed no differences in % fat between age groups. The results indicated that: (a) dissociations existed between the direct and indirect indicators of adiposity; (b) the wrestlers were similar in height but had smaller upper-body SFs when compared with NHANES samples; and (c) participation in wrestling (1-8 years) had no adverse effects on the normal age-related growth patterns for HT, but favorable effects on measures of adiposity.

Purpose of review Skeletal muscle mass with aging, during critical care, and following critical care is a determinant of quality of life and survival. In this review, we discuss the mechanisms that underpin skeletal muscle atrophy and recommendations to offset skeletal muscle atrophy with aging and during, as well as following, critical care. Recent findings Anabolic resistance is responsible, in part, for skeletal muscle atrophy with aging, muscle disuse, and during disease states. Anabolic resistance describes the reduced stimulation of muscle protein synthesis to a given dose of protein/amino acids and contributes to declines in skeletal muscle mass. Physical inactivity induces: Anabolic resistance (that is likely exacerbated with aging), insulin resistance, systemic inflammation, decreased satellite cell content, and decreased capillary density. Critical illness results in rapid skeletal muscle atrophy that is a result of both anabolic resistance and enhanced skeletal muscle breakdown. Summary Insofar as atrophic loss of skeletal muscle mass is concerned, anabolic resistance is a principal determinant of age-induced losses and appears to be a contributor to critical illness-induced skeletal muscle atrophy. Older individuals should perform exercise using both heavy and light loads three times per week, ingest at least 1.2 g of protein/kg/day, evenly distribute their meals into protein boluses of 0.40 g/kg, and consume protein within 2 h of retiring for sleep. During critical care, early, frequent, and multimodal physical therapies in combination with early, enteral, hypocaloric energy (∼10-15 kcal/kg/day), and high-protein (>1.2 g/kg/day) provision is recommended.

This study examined the time courses of recovery for isometric peak torque and rate of torque development (RTD) after eccentric-induced muscle damage. 18 men completed 6 sets of 10 maximal eccentric isokinetic muscle actions at 30° · s(-1). Peak torque, peak RTD and RTD at 10 (RTD10), 50 (RTD50), 100 (RTD100) and 200 ms (RTD200), serum creatine kinase and lactate dehydrogenase were measured before (PRE), immediately after (POST), 24, 48 and 72 h after eccentric exercise. Creatine kinase and lactate dehydrogenase increased from 139 to 6 457 and from 116 to 199 IU · L(-1) from PRE to 72 h, respectively. Peak torque and all RTDs decreased at POST. Peak torque and RTD200 remained lower than PRE through 72 h. Peak RTD remained lower than PRE through 48 h, but was not different from PRE at 72 h. RTD10 and RTD100 were lower than PRE through 24 h, but were not different from PRE at 48 and 72 h. RTD50 decreased at POST, but was not different from PRE at 24 h. Early phase RTDs recovered more quickly than PT and RTD200. Early phase RTDs may reflect neural mechanisms underlying eccentric-induced force decrements, while late RTDs may describe the same physiological mechanisms as PT.

OBJECTIVE: This study examined the effects of sex on peak torque (PT), average power (AP), and the time (AMP) and frequency (MPF) domain parameters of the electromyographic (EMG) and mechanomyographic (MMG) signals following very short-term resistance training (VST) of the forearm flexors. Based on the results of previous studies it was hypothesized that three training sessions would increase PT, EMG MPF, and MMG MPF of the forearm flexors at 60 and 180 ◦ /s in men only, without changes to AP, EMG AMP, or MMG AMP for either men or women. METHODS: Nine men and nine women completed two pretests, three training sessions, and a posttest that included concentric isokinetic muscle actions of the forearm flexors at 60 and 180 ◦ /s. The AMP and MPF of the EMG and MMG signals were recorded from the biceps brachii. RESULTS: The results indicate increases in PT, AP, and MMG AMP at 60 and 180 ◦ /s for the men only, but no changes in EMG AMP, EMG MPF, or MMG MPF for either the men or women. CONCLUSION: There were gender differences in the PT and AP responses to VST of the forearm flexors that were not associated with increased agonist muscle activation. These findings have implications for the development of gender-specific VST programs for the forearm flexors in clinical settings.

Estimates of critical power (CP) and anaerobic work capacity (AWC) from the power output vs. time relationship have been derived from various mathematical models. The purpose of this study was to examine estimates of CP and AWC from the multiple work bout, 2- and 3-parameter models, and those from the 3-minute all-out CP (CP3min) test. Nine college-aged subjects performed a maximal incremental test to determine the peak oxygen consumption rate and the gas exchange threshold. On separate days, each subject completed 4 randomly ordered constant power output rides to exhaustion to estimate CP and AWC from 5 regression models (2 linear, 2 nonlinear, and 1 exponential). During the final visit, CP and AWC were estimated from the CP3min test. The nonlinear 3-parameter (Nonlinear-3) model produced the lowest estimate of CP. The exponential (EXP) model and the CP3min test were not statistically different and produced the highest estimates of CP. Critical power estimated from the Nonlinear-3 model was 14% less than those from the EXP model and the CP3min test and 4-6% less than those from the linear models. Furthermore, the Nonlinear-3 and nonlinear 2-parameter (Nonlinear-2) models produced significantly greater estimates of AWC than did the linear models and CP3min. The current findings suggested that the Nonlinear-3 model may provide estimates of CP and AWC that more accurately reflect the asymptote of the power output vs. time relationship, the demarcation of the heavy and severe exercise intensity domains, and anaerobic capabilities than will the linear models and CP3min test.

This study examined acute physiologic responses to a thermogenic nutritional supplement at rest, during exercise, and during recovery from exercise in women. Twelve women (mean ± SD age, 22.9 ± 3.1 years) were recruited for this randomized, double-blinded, placebo-controlled, crossover study. Each testing session consisted of 4 phases: 30 min of presupplementation resting, followed by the ingestion of the placebo or thermogenic nutritional supplement; 50 min of postsupplementation resting; 60 min of walking (at 3.2–4.8 km·h−1); and 50 min of postexercise resting. Energy expenditure (EE), oxygen consumption, respiratory exchange ratio (RER), oxygen (O2) pulse, and heart rate (HR) values were recorded during all 4 phases. Systolic (SBP) and diastolic (DBP) blood pressure were recorded during the rest, postsupplementation, and postexercise recovery phases; ratings of perceived exertion (RPE) were recorded only during exercise. There were no significant differences for EE, oxygen consumption, O2 pulse, HR, SBP, or DBP between the supplement and placebo during the presupplementation resting or postsupplementation phases. The RER, however, was higher with the supplement at 30 min postsupplementation. During exercise, EE and O2 pulse were 3%–6% greater with the supplement than placebo; there were no significant differences in RPE. Postexercise, EE, oxygen consumption, and DBP were 3%–7% greater with the supplement than placebo. These findings suggest that a thermogenic nutritional supplement, when combined with exercise, increases metabolic rate but has no effect on the perception of effort and results in only minimal changes in cardiovascular function.

Critical power (CP) from the 3-min test was compared to the power outputs associated with thresholds determined from gas exchange parameters that have been used to demarcate the exercise-intensity domains including the respiratory compensation point (RCP), gas exchange threshold (GET), and ventilatory threshold (VT).Twenty-eight participants performed an incremental-cycle ergometer test to exhaustion. The VT was determined from the relationship between the ventilatory equivalent for oxygen uptake (VE/VO2) versus VO2 and the GET was determined using the V-slope method (VCO2 vs. VO2). The RCP was identified from the VE-versus-VCO2 relationship. CP was the average power output during the last 30 s of the 3-min all-out test. Linear regression was used to determine the power outputs associated with the RCP, GET, and VT, as well as the VO2 associated with CP. Mean differences among the associated power outputs, percent VO2 peak, and percent peak power output for the GET, VT, RCP, and CP were analyzed using separate one-way repeated-measures analyses of variance.There were no significant differences between CP (187 +/- 47W) and the power output associated with RCP (190 +/- 49W) or between the power outputs associated with GET (139 +/- 37W) and VT (145 +/- 37W). The power outputs associated with GET and VT, however, were significantly less than were those at CP and associated with RCRP.These findings suggest CP and RCP demarcate the heavy from severe exercise-intensity domain and result from a different mechanism of fatigue than that of GET and VT, possibly hyperkalemia.

The purpose of the present study was to examine the patterns of responses for torque, electromyographic (EMG) amplitude, EMG mean power frequency (MPF), mechanomyographic (MMG) amplitude, and MMG MPF across 30 repeated maximal isometric (ISO) and concentric (CON) muscle actions of the leg extensors. Twelve female subjects (21.1±1.4yrs; 63.3±7.4kg) performed ISO and CON fatigue protocols with EMG and MMG signals recorded from the vastus lateralis. The relationships for torque, EMG amplitude, EMG MPF, MMG amplitude, and MMG MPF versus repetition number were examined using polynomial regression. The results indicated there were decreases (p0.05) across the ISO muscle actions for torque (r(2)=0.95), EMG amplitude (R(2)=0.44), EMG MPF (r(2)=0.62), and MMG MPF (r(2)=0.48), but no change in MMG amplitude (r(2)=0.07). In addition, there were decreases across the CON muscle actions for torque (R(2)=0.97), EMG amplitude (R(2)=0.46), EMG MPF (R(2)=0.86), MMG amplitude (R(2)=0.44), and MMG MPF (R(2)=0.80). Thus, the current findings suggested that the mechanisms of fatigue and motor control strategies used to modulate torque production were similar between maximal ISO and CON muscle actions.

Introduction: We compared peak and submaxi- mal mean values for neuromuscular and metabolic parameters between ramp (15 Wmin � 1 ) and step (30 W increments every 2 min) incremental cycle ergometer tests. Methods: Thirteen healthy adults (7 men and 6 women; mean 6 SD age ¼ 23.4 6 3.3 years) performed randomly ordered ramp or step incre- mental tests. Two-way repeated measures analyses of variance were used to analyze the data. Results: The ramp incremental test resulted in lower mean EMG amplitude, _ VO2, and HR val- ues at the common power outputs, with no differences for MMG amplitude values. Conclusions: It is possible that the cumula- tive effect of producing an increased amount of work during the step (total work ¼ 75.83 kJ) vs. ramp (total work ¼ 65.60 kJ) incremental cycle ergometer tests at the common power out- puts may have contributed to the greater fatigue-induced increase in muscle recruitment and/or firing rate, oxygen con- sumption, and heart rate. Muscle Nerve 47:555-560, 2013

The purpose of the present study was to examine oxygen consumption rate ([Vdot][Formula: see text]), heart rate (HR), and ratings of perceived exertion (RPE) responses, as well as time to exhaustion (Tlim) values during continuous rides at critical power (CP) determined from the 3-min all-out test. Eighteen participants (mean ± s: 23.6 ± 3.5 years; 72.7 ± 18.2 kg) performed an incremental cycle ergometer test to exhaustion to determine peak oxygen consumption rate ([Vdot][Formula: see text] peak) and HR peak. Critical power was determined from the 3-min all-out test. Metabolic responses ([Vdot][Formula: see text] and heart rate), RPE, and Tlim were recorded during continuous rides to exhaustion at CP. Linear regression and t-tests were used to compare [Vdot][Formula: see text], heart rate, and RPE responses during the continuous rides to exhaustion. The Tlim at CP was 12.5 ± 6.5 min. There were significant increases in [Vdot][Formula: see text], HR, and RPE during the continuous rides at CP and 15 of the 18 participants reached [Vdot][Formula: see text] peak at exhaustion. Therefore, the [Vdot][Formula: see text], heart rate, and RPE responses, as well as the Tlim values in the present study suggested that CP determined from the 3-min all-out test overestimated the "true" CP and was within the severe exercise intensity domain.

OBJECTIVE: The purpose of the present study was to examine the effects of three sessions of concentric isokinetic training of the forearm flexors on peak torque (PT) at maximal voluntary isometric contraction (MVIC), 60, 180, and 300 ◦ · s −1 in males. METHODS: Ten adult males (mean age ± SD = 21.8 ± 1.2 years; body mass = 87.8 ± 18.4 kg; height = 182 ± 9.5 cm) completed two pretests (pretest 1 and pretest 2) and a posttest that included maximal unilateral isometric and concentric isokinetic forearm flexion (non-dominant arm) muscle actions at MVIC, 60, 180, and 300 ◦ · s −1 . During the three days of training, the subjects performed five sets of ten maximal isokinetic concentric forearm flexion (non-dominant arm) repetitions at 60 ◦ · s −1 . RESULTS: A three (pretest 1, pretest 2, and posttest) x four (MVIC, 60, 180, 300 ◦ · s −1 ) repeated measures ANOVA indicated that there was no significant ( p> 0.05) interaction, but significant main effects for time and velocity. The follow-up t-tests for marginal means indicated that the posttest (63.0 ± 13.1 Nm) was significantly greater than pretest 1 (55.8 ± 15.1 Nm) and pretest 2 (55.2 ± 12.4 Nm). CONCLUSION: Thus, the very short-term resistance training (VST) resulted in the same pattern of increase in PT at each velocity. In addition, PT decreased significantly with velocity. The current findings indicated that three days of concentric isokinetic trainingfor theforearm flexors weresufficient toelicit increases inPTacross a velocityspectrum (MVIC to300 ◦ ·s −1 ).

OBJECTIVE: This cross-sectional study examined the relationships between age and absolute peak torque (PT) as well as PT covaried for body weight (BW), height (HT), and/or fat-free weight (FFW) in young girl swimmers. METHODS: Twenty-nine girl swimmers (age = 12.4 ± 1.9 yr) were measured for isokinetic elbow flexion and extension PT at 30, 180, 300 ◦ · s −1 , as well as BW, HT, and FFW. Zero-order correlations as well as first, second, and third-order partial correlations were used to determine the relationships for age versus PT and age versus PT covaried for BW, HT, and/or FFW. RESULTS:There were age-related increases for all absolute PT measures (r = 0.47 to 0.77), except elbow flexion at 300 ◦ · s −1 . For all but one of the measures (forearm extension at 30 ◦ · s −1 ; r = 0.42 to 0.56), the increases in PT were accounted for by changes in BW, HT, and/or FFW. CONCLUSION: The results of this study indicated that agef-related increases in PT for four of the six measures were accounted for by BW, HT, and/or FFW. The age-related increases in PT for elbow flexion at 30 ◦ · s −1 independent of BW, HT, and/or FFW

The purpose of this study was to examine mean differences and the patterns of responses for oxygen uptake ([Formula: see text]O2), heart rate (HR), and rating of perceived exertion (RPE) for ramp (15 W·min–1) versus step (30 W increments every 2 min) incremental cycle ergometer tests. Fourteen subjects (age and body mass of 23.2 ± 3.1 (mean ± SD ) years and 71.1 ± 10.1 kg, respectively) visited the laboratory on separate occasions. Two-way repeated measures ANOVAs with appropriate follow-up procedures, as well as paired t tests, were used to analyze the data. In addition, polynomial regression analyses were used to determine the patterns of responses for each dependent variable for the ramp and step tests. The ramp protocol resulted in lower mean [Formula: see text]O2 and HR values at the common power outputs than the step protocol with no differences in RPE. The increased amount of work performed during the step (total work = 75.83 kJ) versus ramp (total work = 65.60 kJ) tests at the common power outputs may have contributed to the greater [Formula: see text]O2 and HR values. The polynomial regression analyses showed that most subjects had the same patterns of responses for the ramp and step incremental tests for HR (86%) and RPE (93%) but different patterns for [Formula: see text]O2 (71%). The findings from the present study suggested that the protocol selection for an incremental cycle ergometer test can affect the mean values for [Formula: see text]O2 and HR, as well as the [Formula: see text]O2 – power output relationship.

The purpose of this study was to develop a 3-minute, all-out test protocol using the Monark cycle ergometer for estimating the critical power (CP) and anaerobic work capacity (AWC) with the resistance based on body weight. Twelve moderately trained adults (mean age ± SD = 23.2 ± 3.5 years) performed an incremental cycle ergometer test to exhaustion. The CP and AWC were estimated from the original work limit (W(lim)) vs. time limit (T(lim)) relationship (CP(PT)) and a 3-minute all-out test (CP(3min)) against a fixed resistance and compared with the CP and AWC estimated from the new 3-minute tests on the Monark cycle ergometer (CP(3.5%) and CP(4.5%)). The resistance values for the CP(3.5%) and CP(4.5%) tests were set at 3.5 and 4.5% of the subject's body weight (kilograms). The results indicated that there were no significant differences (p > 0.05) among mean CP values for CP(PT) (178 ± 47 W), CP(3.5%) (173 ± 40 W), and CP(4.5%) (186 ± 44 W). The mean CP(3min) (193 ± 54 W), however, was significantly greater than CP(PT) and CP(3.5%). There were no significant differences in AWC for the CP(PT) (13,412 ± 6,247 J), CP(3min) (10,895 ± 2,923 J), and CP(4.5%) (9,842 ± 4,394 J). The AWC values for the CP(PT) and CP(3min), however, were significantly greater than CP(3.5%) (8,357 ± 2,946 J). The results of this study indicated that CP and AWC could be estimated from a single 3-minute work bout test on the Monark cycle ergometer with the resistance set at 4.5% of the body weight. A single work bout test with the resistance based on the individual's body weight provides a practical and accessible method to estimate CP and AWC.

The purpose of this study was to examine the effects of anatabine supplementation in conjunction with unilateral, maximal eccentric isokinetic muscle actions on serum markers of muscle damage and pro-inflammatory cytokines in humans. Seventeen men (mean ± S.D. age = 22.4 ± 3.2 yrs) participated in this double-blinded, placebo-controlled, crossover study. Participants were randomly assigned to two 10-day conditions (anatabine and placebo) separated by a 2-4 week washout period. After seven days of supplementation, blood was sampled immediately prior to PRE, immediately following POST, and 24, 48, and 72 h after 6 sets of 10 repetitions of unilateral, maximal eccentric isokinetic forearm flexion exercise. Concentrations of serum creatine kinase, lactate dehydrogenase, myoglobin, high sensitivity c-reactive protein, and TNF-α were measured. Creatine kinase, myoglobin, and lactate dehydrogenase increased (P0.05), while high sensitivity c-reactive protein and TNF-α did not change (P0.05) after the eccentric exercise during both conditions. Lactate dehydrogenase was higher (P0.05) during the anatabine condition. The primary findings of this study were two-fold: (a) anatabine had no beneficial effects on traditional markers of muscle damage (creatine kinase, lactate dehydrogenase, and myoglobin) compared to placebo after the eccentric exercise protocol, and (b) the eccentric exercise protocol did not elicit increase in the pro-inflammatory cytokines (c-reactive protein and TNF-α). Future studies are needed to examine the effects of anatabine on naturally-occurring inflammation that is common with aging or obesity. Furthermore, additional research is needed to examine the relationship between muscle damage and inflammation after eccentric exercises of different modes, durations, and intensities.

This study examined the relationships among the physical working capacity at the fatigue threshold (PWCFT), the power outputs associated with the gas exchange threshold (PGET) and the respiratory compensation point (PRCP), and critical power (CP) to identify possible physiological mechanisms underlying the onset of neuromuscular fatigue. Ten participants (mean ± SD age: 20 ± 1 years) performed a maximal incremental cycle ergometer test to determine the PWCFT, PGET, and PRCP. CP was determined from the 3 min all-out test. The PWCFT (197 ± 55 W), PRCP (212 ± 50 W), and CP (208 ± 63 W) were significantly greater than the PGET (168 ± 40 W), but there were no significant differences among the PWCFT, PRCP, and CP. All thresholds were significantly inter-4 (r = 0.794–0.958). The 17% greater estimates for the PWCFT than PGET were likely related to differences in the physiological mechanisms that underlie these fatigue thresholds, while the non-significant difference and high correlation between the PWCFT and the PRCP suggested that hyperkalemia may underlie both thresholds. Furthermore, it is possible that the 5% lower estimate of the PWCFT than CP could more accurately reflect the demarcation of the heavy from severe exercise intensity domains.

The purpose of this study was to examine the effects of 28 days of polyethylene glycosylated creatine (PEG-creatine) supplementation (1.25 and 2.50 g·d) on anaerobic performance measures (vertical and broad jumps, 40-yard dash, 20-yard shuttle run, and 3-cone drill), upper- and lower-body muscular strength and endurance (bench press and leg extension), and body composition. This study used a randomized, double-blind, placebo-controlled parallel design. Seventy-seven adult men (mean age ± SD, 22.1 ± 2.5 years; body mass, 81.7 ± 10.8 kg) volunteered to participate and were randomly assigned to a placebo (n = 23), 1.25 g·d of PEG-creatine (n = 27), or 2.50 g·d of PEG-creatine (n = 27) group. The subjects performed anaerobic performance measures, muscular strength (one-repetition maximum [1RM]), and endurance (80% 1RM) tests for bench press and leg extension, and underwater weighing for the determination of body composition at day 0 (baseline), day 14, and day 28. The results indicated that there were improvements (p < 0.0167) in vertical jump, 20-yard shuttle run, 3-cone drill, muscular endurance for bench press, and body mass for at least one of the PEG-creatine groups without changes for the placebo group. Thus, the present results demonstrated that PEG-creatine supplementation at 1.25 or 2.50 g·d had an ergogenic effect on lower-body vertical power, agility, change-of-direction ability, upper-body muscular endurance, and body mass.

The purpose of this study was to determine the specific metabolic and neuromuscular responses at critical power (CP) from the 3-min all-out test. Nine men (mean ± SD: aged 23.7 ± 3.3 years) performed an incremental test for the determination of peak oxygen consumption (V̇O2peak) and gas exchange threshold. CP was estimated for each subject from the 3-min all-out test. Oxygen consumption (V̇O2), the ventilation versus carbon dioxide production ratio (V̇E/V̇CO2 ratio), electromyographic (EMG) amplitude, and EMG mean power frequency (MPF) were examined during exhaustive rides at CP for each subject. There was no significant difference between the V̇O2 at exhaustion (40.6 ± 7.5 mL·kg−1·min−1) and V̇O2peak (42.9 ± 7.3 mL·kg−1·min−1). Furthermore, there were significant increases in EMG amplitude and the V̇E/V̇CO2 ratio during the exhaustive rides at CP. There was, however, no significant change in EMG MPF over time. Therefore, the current findings indicated that the 3-min all-out test overestimated CP and the demarcation between the heavy- and severe-intensity domains. Specifically, the V̇O2, ventilatory, and EMG amplitude responses were consistent with those observed during continuous exercise in the severe exercise intensity domain. It is likely that the ventilatory and EMG amplitude responses were associated with a common mechanism of fatigue that is different from what affects EMG MPF.

We compared peak and submaximal mean values for neuromuscular and metabolic parameters between ramp (15 W · min(-1)) and step (30 W increments every 2 min) incremental cycle ergometer tests.Thirteen healthy adults (7 men and 6 women; mean ± SD age = 23.4 ± 3.3 years) performed randomly ordered ramp or step incremental tests. Two-way repeated measures analyses of variance were used to analyze the data.The ramp incremental test resulted in lower mean EMG amplitude, O2, and HR values at the common power outputs, with no differences for MMG amplitude values.It is possible that the cumulative effect of producing an increased amount of work during the step (total work = 75.83 kJ) vs. ramp (total work = 65.60 kJ) incremental cycle ergometer tests at the common power outputs may have contributed to the greater fatigue-induced increase in muscle recruitment and/or firing rate, oxygen consumption, and heart rate.

There are limited data regarding metabolic responses during continuous exhaustive rides at critical power (CP) from the 3-min all-out test. In addition, no previous studies have examined the mechanomyographic (MMG) responses at CP from the 3-min all-out test. Therefore, this study examined the metabolic and MMG responses during continuous exercise at CP determined from the 3-min all-out test. Nine college-aged females (mean±SD: age 23.0±3.6yrs) performed an incremental test to exhaustion on a cycle ergometer to identify the gas exchange threshold, peak oxygen consumption rate (V˙O2 peak) and heart rate peak (HR peak). The V˙O2, HR, MMG amplitude and mean power frequency (MPF) responses were examined during continuous rides to exhaustion at CP (81±6% peak power). There were significant increases in V˙O2 and HR over time and there was no significant difference between V˙O2 peak and V˙O2 at exhaustion or HR peak and HR at exhaustion. There were, however, no significant changes for MMG amplitude or MPF over time. Therefore, the current findings suggested that the 3-min all-out test overestimated CP and the demarcation between the heavy and severe intensity domains. Specifically, the V˙O2 and HR responses did not reach a steady state and were driven to peak values. Furthermore, the non-significant change in MMG amplitude and MPF were consistent with the responses observed at fatiguing power outputs (i.e., >80% peak power).

The purposes of this study were to compare the power outputs and estimated times to exhaustion (Tlim) at the gas exchange threshold (GET), physical working capacity at the rating of perceived exertion threshold (PWCRPE), and respiratory compensation point (RCP). Three male and 5 female subjects (mean ± SD: age, 22.4 ± 2.8 years) performed an incremental test to exhaustion on an electronically braked cycle ergometer to determine peak oxygen consumption rate, GET, and RCP. The PWCRPE was determined from ratings of perceived exertion data recorded during 3 continuous workbouts to exhaustion. The estimated Tlim values for each subject at GET, PWCRPE, and RCP were determined from power curve analyses (Tlim = axb). The results indicated that the PWCRPE (176 ± 55 W) was not significantly different from RCP (181 ± 54 W); however, GET (155 ± 42 W) was significantly less than PWCRPE and RCP. The estimated Tlim for the GET (26.1 ± 9.8 min) was significantly greater than PWCRPE (14.6 ± 5.6 min) and RCP (11.2 ± 3.1 min). The PWCRPE occurred at a mean power output that was 13.5% greater than the GET and, therefore, it is likely that the perception of effort is not driven by the same mechanism that underlies the GET (i.e., lactate buffering). Furthermore, the PWCRPE and RCP were not significantly different and, therefore, these thresholds may be associated with the same mechanisms of fatigue, such as increased levels of interstitial and (or) arterial [K+].

The purpose of this study was to determine if Critical Power (CP) and Anaerobic Work Capacity (AWC) could be estimated from a single, all-out test of less than 3-min. Twenty-eight subjects (mean ± SD: age 23.3 ± 3.3 years, body mass 71.6 ± 16 kg) performed an incremental cycle ergometer test to exhaustion to determine peak oxygen consumption rate and heart rate peak. The 3-min all-out test was used to determine the criterion and six estimated values of CP and AWC. The criterion critical power (CP 180 ) and anaerobic work capacity (AWC 180 ) values were determined from the 3-min all-out test and were expressed as 30-s averages (155-180-s). The six estimated CP and AWC values were calculated from 30-s averages at decreasing 10-s intervals from 145 to 170-s (CP 170 and AWC 170 ), 135 to 160-s (CP 160 and AWC 160 ), 125 to 150-s (CP 150 and AWC 150 ), 115 to 140-s (CP 140 and AWC 140 ), 105 to 130-s (CP 130 and AWC 130 ), and 95 to 120-s (CP 120 and AWC 120 ). Mean differences, total error, constant error, standard error of the estimate, and correlations were used to compare the criterion to the estimated CP and AWC values. The results of the present study indicated that 150-s was the shortest test duration that resulted in non-significant differences between the criterion (CP 180 and AWC 180 ) and estimated CP (CP 150 ) and AWC (AWC 150 ) values. The subsequent validation analyses showed that there were close agreements for the estimated CP 150 and AWC 150 versus the criterion (CP 180 and AWC 180 ) values. Therefore, the current findings indicated that estimates of CP and AWC were not affected by shortening the test by 30-s. Reducing the length of the test to 2.5 minutes provides a less strenuous, yet valid protocol for estimating CP and AWC.

Background Limited data are available examining dietary interventions for optimizing protein and leucine intake to stimulate muscle protein synthesis (MPS) in older humans. Objectives We aimed to investigate the aminoacidemia and appetite responses of older adults after consuming breakfast, a meal frequently consumed with high-carbohydrate and below-par amounts of protein and leucine for stimulating MPS. Methods Five men and 3 women (means ± SD; age: 74 ± 7 y, BMI: 25.7 ± 4.9 kg/m2, fat- and bone-free mass: 63 ± 7 kg) took part in this experiment in which they consumed breakfasts with low-protein (LP = 13 ± 2 g), high-protein (HP = 32 ± 5 g), and LP followed by a protein- and leucine-enriched bar formulation 2 h later (LP + Bar = 29 ± 2 g). The LP, HP, and LP + Bar breakfast conditions contained 519 ± 86 kcal, 535 ± 83 kcal, and 739 ± 86 kcal, respectively. Blood samples were drawn for 6 h and analyzed for amino acid, insulin, and glucose concentrations. Visual analog scales were assessed for hunger, fullness, and desire to eat. Results The net AUC for essential amino acid (EAA) exposure was similar between the LP + Bar and HP conditions but greater in the HP condition compared with the LP condition. Peak leucinemia was higher in the LP + Bar condition compared with the HP, and both were greater than the LP condition. Net leucine exposure was similar between HP and LP + Bar, and both were greater than LP. Hunger was similarly reduced in LP + Bar and HP, and LP + Bar resulted in a greater hunger reduction than LP. Both LP + Bar and HP resulted in greater net fullness scores than LP. Conclusions Consuming our bar formulation increased blood leucine availability and net exposure to EAAs to a similar degree as consuming a high-protein meal. High-protein at breakfast results in a greater net exposure to EAAs and leucine, which could support MPS in older persons. This study was registered at clinicaltrials.gov as NCT03712761., There are practical limitations for older persons to sufficiently increase daily protein intake. Dietary recommendations aimed to prevent or minimize sarcopenia may benefit from inclusion of supplementing with unique formulations containing milk proteins with free leucine.

Background Anatabine (ANA), a minor tobacco alkaloid found in the Solanaceae family of plants, may exhibit anti-inflammatory activity, which may be useful to aid in recovery from exercise-induced muscle damage. The purpose of this study, therefore, was to examine the effects of ANA supplementation on the recovery of isometric strength and selected non-invasive indicators of muscle damage. Methods A double-blinded, placebo-controlled, crossover design was used to study eighteen men (mean ± SD age = 22.2 ± 3.1 yrs; body mass = 80.3 ± 15.7 kg) who participated in two randomly-ordered conditions separated by a washout period. The ANA condition consisted of consuming 6–12 mg anatabine per day for 10 days, while testing took place during days 7–10. The placebo (PLA) condition was identical except that the PLA supplement contained no ANA. Maximal voluntary isometric peak torque (PT) of the forearm flexors, arm circumference, hanging joint angle, and subjective pain ratings were measured before (PRE), immediately after (POST), and 24, 48, and 72 h after six sets of 10 maximal, eccentric isokinetic forearm flexion muscle actions. Resting heart rate and blood pressure were measured at PRE and 72 h in each condition. Results For PT, hanging joint angle, arm circumference, and subjective pain ratings, there were no condition x time (p > 0.05) interactions, there were no main effects for condition (p > 0.05), but there were main effects for time (p 0.05) interactions and no main effects for condition (p > 0.05) or time (p > 0.05) for blood pressure or resting heart rate. Conclusions ANA supplementation had no effect on the recovery of muscle strength, hanging joint angle, arm swelling, or subjective pain ratings after a bout of maximal eccentric exercise in the forearm flexors. Therefore, ANA may not be beneficial for those seeking to improve recovery from heavy eccentric exercise. Future studies should examine the effects of ANA on the pro-inflammatory cytokine responses to exercise-induced muscle damage and the chronic low-grade inflammation observed in obese and elderly individuals.