1. The m6A RNA Modification Modulates Gene Expression and Fibrosis-Related Pathways in Hypertrophic Scar
- Author
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Si-Yu Liu, Jun-Jie Wu, Zhong-hua Chen, Ming-Li Zou, Ying-ying Teng, Kai-Wen Zhang, Yue-Yue Li, Dang-yang Guo, and Feng-Lai Yuan
- Subjects
hypertrophic scar ,m6A sequencing ,modification patterns ,N6-methyladenosine ,MeRIP-seq ,Biology (General) ,QH301-705.5 - Abstract
Purpose: To systematically analyze the overall m6A modification pattern in hyperplastic scars (HS).Methods: The m6A modification patterns in HS and normal skin (NS) tissues were described by m6A sequencing and RNA sequencing, and subsequently bioinformatics analysis was performed. The m6A-related RNA was immunoprecipitated and verified by real-time quantitative PCR.Results: The appearance of 14,791 new m6A peaks in the HS sample was accompanied by the disappearance of 7,835 peaks. The unique m6A-related genes in HS were thus associated with fibrosis-related pathways. We identified the differentially expressed mRNA transcripts in HS samples with hyper-methylated or hypo-methylated m6A peaks.Conclusion: This study is the first to map the m6A transcriptome of human HS, which may help clarify the possible mechanism of m6A-mediated gene expression regulation.
- Published
- 2021
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