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29 results on '"Dananjaya, S.H.S."'

24. Chitosan Silver Nano Composites (CAgNCs) as Antibacterial Agent Against Fish Pathogenic Edwardsiella tarda

Author

G.I. Godahewa, Mahanama De Zoysa, Jehee Lee, Dananjaya S.H.S, Jongki Cho, and Youngdeuk Lee

Subjects
Materials science, Minimum bactericidal concentration, General Veterinary, biology, Edwardsiella tarda, Silver Nano, Pathogenic bacteria, biology.organism_classification, medicine.disease_cause, Bacterial cell structure, Microbiology, Minimum inhibitory concentration, medicine, Composite material, skin and connective tissue diseases, Bacteria, Antibacterial agent
Abstract

Recently nano particles have proven for wide array of bioactive properties. In the present study, antibacterial properties of chitosan silver nano composites (CAgNCs) were investigated against fish pathogenic Edwardsiella tarda. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs against E. tarda were 25 μg/mL and 125 μg/mL, respectively. The field emission scanning electron microscope (FE-SEM) image of CAgNCs treated E. tarda showed the strongly damaged bacteria cells than non-treated bacteria. Furthermore, treatment of CAgNCs induced the level of intracellular reactive oxygen species (ROS) in E. tarda cells in concentration and time dependent manner suggesting that it may generate oxidative stress leading to bacterial cell death. In addition, MTT assay results showed that the lowest cell viability at 100 μg/mL of CAgNCs treated E. tarda. Overall results of this study suggest that CAgNCs is a potential antibacterial agent to control pathogenic bacteria.

Published
2014
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25. INHIBITION ACTION OF LAWSONE ON THE CORROSION OF MILD STEEL IN ACIDIC MEDIA

Author

DANANJAYA, S.h.s., EDUSSURİYA, M., and DİSSANAYAKE, A.s.

Subjects
technology, industry, and agriculture, Corrosion inhibition,Hydrochloric acid,Mild steel,2-hydroxy-1,4-napthoquinone
Abstract

The use of inhibitors is one of the most practical methods for protection against corrosion. Lawsone, 2-hydroxy-1,4-naphthoquinone, the main active ingredient of Henna (Lawsonia inermis) plantwas extracted, isolated and subjected to several studies. The corrosion inhibition of lawsone on the corrosion of mild steel in 1.0 M hydrochloric acid was studied using weight loss method. It was found that lawsone acts as a good corrosion inhibitor for mild steel in 1.0 M HCl medium. The inhibition efficiency increases with increasing of lawsone concentration. The inhibitive action was addressed in view of adsorption of lawsone molecules on the metal surface. It was found that this adsorption follows Langmuir adsorption isotherm in all tested systems and the adsorption is not activated

Published
2016

27. Growth performance and color enhancement of goldfish, Carassius auratus, fed diets containing natural dyes extracted from annatto (Bixa orellana) seeds.

Author

Dananjaya, S.H.S, Manjula, Prabuddha, Dissanayake, A. S., Edussuriya, M., Radampola, K., Park, Bae Keun, and De Zoysa, Mahanama

Subjects
*GOLDFISH, *NATURAL dyes & dyeing, *FISH skin, *FISH nutrition, *FISH food, *FISH feeds
Abstract

The present study was conducted to evaluate growth performance and color enhancement of goldfish, Carassius auratus, fed diets containing 0, 50, 100, 200, and 250 mg kg−1 diet of annatto dye (AD) for 60 days. The survival rate was significantly higher in fish fed 100, 200, and 250 mg AD kg−1 diet over than these fed control and 50 mg AD kg−1 diet (p < 0.05). AD significantly (p <0.05) increased the pigmentation in the skin and caudal fin of goldfish in a concentration dependent manner (R2 = 0.995, 0.997). The highest amount of total carotenoid deposition in fish skin and fins were given by diets containing 200–250 mg AD kg−1 diet. The highest redness (a*) of 43.21 and yellowness (b*) of 12.53 were obtained by 250 and 50 mg AD kg−1, respectively. The present results show that AD can be successfully used as an alternative natural carotenoid source in goldfish diets at levels of 200–250 mg AD kg−1 diet. [ABSTRACT FROM AUTHOR]

Published
2020
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29. Natural bixin as a potential carotenoid for enhancing pigmentation and colour in goldfish ( Carassius auratus).

Author

Dananjaya, S.H.S., Munasinghe, D.M.S., Ariyaratne, H.B.S., Lee, J., and De Zoysa, M.

Subjects
*CAROTENOIDS, *GOLDFISH, *FISH feeds, *DIETARY supplements, *FEED utilization efficiency, *FISH growth, *PHYSIOLOGY
Abstract

Discovering natural carotenoids for colour enhancement and health benefits of fish is important to develop new feed formulations. We have purified natural bixin from achiote seeds and evaluated the effect of colour enhancing and pigmentation in goldfish. Varying levels of bixin-based diets were prepared with 420 g kg−1 of crude protein and 120 g kg−1 of lipid content. Our results clearly showed that bixin (0.05, 0.10, 0.20 and 0.60 g kg−1) based diets significantly ( P < 0.05) enhanced the skin and fin colour at 30 and 60 days compared to diet without bixin. Interestingly, diet which contains 0.20 g kg−1 bixin and commercial feed (with astaxanthin) had similar effect on carotenoid deposition in skin. Moreover, total carotenoid deposition in fin was higher than in skin of all bixin-containing diets. However, 0.60 g kg−1 bixin-containing diet had lower specific growth rate (1.01 ± 0.01) and higher feed conversion ratio (2.05 ± 0.19) compared to the control group. The present results demonstrate that achiote bixin can be successfully used as an alternative natural carotenoid source against synthetic astaxanthin in fish feed. Our data indicate that 0.20 g kg−1 is a suitable dietary level of bixin to ensure strong pigmentation, acceptable growth and feed utilization in goldfish. [ABSTRACT FROM AUTHOR]

Published
2017
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