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Abstract Podocytes are highly specialized cells playing a key role in the filtration function of the kidney. A damaged podocyte ultrastructure is associated with a reorganization of the actin cytoskeleton and accompanied with a loss of adhesion to the glomerular basement membrane leading to proteinuria in many forms of glomerular diseases, e.g. nephrotic syndrome. If the first-line therapy with glucocorticoids fails, alternative immunosuppressive agents are used, which are known to have the potential to stabilize the actin cytoskeleton. A new option for preventing relapses in steroid dependent nephrotic syndrome is the monoclonal antibody rituximab, which, in addition to its B-cell depleting effect, is assumed to have direct effects on podocytes. We here provide data on the non-immunological off-target effects of the immunosuppressant rituximab on podocyte structure and dynamics in an in vitro puromycin aminonucleoside model of podocyte injury. A conditionally immortalized human podocyte cell line was used. Differentiated podocytes were treated with puromycin aminonucleoside and rituximab. Our studies focussed on analyzing the structure of the actin cytoskeleton, cellular adhesion and apoptosis using immunofluorescence staining and protein biochemistry methods. Treatment with rituximab resulted in a stabilization of podocyte actin stress fibers in the puromycin aminonucleoside model, leading to an improvement in cell adhesion. A lower apoptosis rate was observed after parallel treatment with puromycin aminonucleoside and rituximab visualized by reduced nuclear fragmentation. Consistent with this data, Western-blot analyses demonstrated that rituximab directly affects the caspase pathways by inhibiting the activation of Caspases-8, -9 and -3, suggesting that rituximab may inhibit apoptosis. In conclusion, our results indicate an important role of the immunosuppressant rituximab in terms of stability and morphogenesis of podocytes, involving apoptosis pathways. This could help to improve therapeutical concepts for patients with proteinuria mediated by diseased podocytes.

Background: Wave energy represents one of the most promising renewable energies due to its great theoretical potential. Nevertheless, the electrical compliance of grid-connected systems is a great issue nowadays, due to the highly stochastic nature of wave energy. Methods: In this paper, a Hybrid Energy Storage System (HESS) consisting of a Li-ion battery and a flywheel is coupled to a Wave Energy Converter (WEC) that operates in grid connected mode. The study is performed using real yearly wave power profiles relating to three different sites located along the European coasts. The Simultaneous Perturbation Stochastic Approximation (SPSA) principle is implemented as real-time power management strategy for HESS in wave energy conversion systems. Results: Obtained results demonstrate how the proposed HESS and the implementation of the SPSA power management coupled to a WEC allow a reduction of more than 80% of power oscillations at the Point of Common Coupling (PCC), while proving the robustness of the developed management strategy over the investigated sites. Moreover, the average energy penalty due to the HESS integration results slightly higher than 5% and battery solicitation is reduced by more than 64% with respect to the flywheel solicitation, contributing to extend its lifetime. Conclusions: HESS integration in renewable generation systems maximizes the WEC production while smoothing the power at the PCC. Specifically, flywheel-battery HESS together with the implemented power management strategy could provide a great flexibility in the view of increasing power production from waves, strongly mitigating the variability of this source while enhancing grid safety and stability.

Objectives. We aimed to assess the presence of MetS and traditional CV risk factors in a group of RA patients, compared to controls and their possible inter-relation with disease activity variables. Methods. We performed an observational study on 38 consecutive patients diagnosed with RA in Rheumatology Department of the Emergency County Hospital Craiova, based on ACR/EULAR criteria, in a one year interval between 2019-2020, and a control group including 30 subjects. Patients’ data were obtained from each subject according to the study protocol and included demographic, clinical, laboratory parameters. The presence of MetS was assessed according to the National Cholesterol Education Program Adult Treatment Panel (NCPATP) III. Results. Regarding the components of metabolic syndrome, as defined by NCPATP III, the differences established for the RA vs control groups were: increased waist circumference in 21 (52.25%) vs 13 (43.33%) subjects (p=0.002); high triglycerides (or under treatment) in 10 (26.31%) vs 6 (20%) subjects, p=0.004; low HDL cholesterol in 15 (39.47%) vs 7 (23.33%) subjects, p=0.002; high blood pressure (or under treatment) in 25 (65.79%) vs 12 (40%) subjects, p

Identifying certain serum biomarkers associated with the degree of rheumatoid arthritis (RA) activity can provide us with a more accurate view of the evolution, prognosis, and future quality of life for these patients. Our aim was to analyze the presence and clinical use of matrix metalloproteinase-13 (MMP-13), along with vascular endothelial growth factor (VEGF) and well-known cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) for patients with RA. We also wanted to identify the possible correlations between MMP-13 and these serological markers, as well as their relationship with disease activity indices, quality of life, and ultrasonographic evaluation. For this purpose, we analyzed serum samples of 34 RA patients and 12 controls. In order to assess serum concentrations for MMP-13, VEGF, TNF-α, and IL-6, we used the enzyme-linked immunosorbent assay (ELISA) technique. Our results concluded that higher levels of MMP-13, VEGF, TNF-α, and IL-6 were present in the serum of RA patients compared to controls, with statistical significance. We furthermore identified moderately positive correlations between VEGF, MMP-13, and disease activity indices, as well as with the ultrasound findings. We also observed that VEGF had the best accuracy (97.80%), for differentiating patients with moderate disease activity. According to the data obtained in our study, that although MMP-13, TNF-α and C-reactive protein (CRP) have the same sensitivity (55.56%), MMP-13 has a better specificity (86.67%) in the diagnosis of patients with DAS28(4v) CRP values corresponding to moderate disease activity. Thus, MMP-13 can be used as a biomarker that can differentiate patients with moderate or low disease activity. VEGF and MMP-13 can be used as additional parameters, along with TNF-α and IL-6, that can provide the clinician a better picture of the inflammatory process, disease activity, and structural damage in patients with RA. Our data can certainly constitute a start point for future research and extended studies with multicenter involvement, to support the selection of individualized and accurate therapeutic management strategies for our patients.

In the present paper an ultrafiltration experiment was carried out for testing the polyacrylonitrile (PAN) membrane in the process of peptone separation from wastewater. The evaluation of the applied ultrafiltration process consisted in the monitoring and the recording of the parameters as well as of some indicators that will reveal the PAN membrane efficiency (the percentage of peptone retention on the membrane surface, the volume and quality of the resulting permeate in the pre-established time period). The obtained results showed that the peptone retention efficiency was noticeably, around 20 % and the obtained permeate volume did not decrease greatly, only by 2.5 L over 300 minutes experiment.

Among Renewable Energy Sources (RES), wind energy is emerging as one of the largest installed renewable-power-generating capacities. The technological maturity of wind turbines, together with the large marine wind resource, is currently boosting the development of offshore wind turbines, which can reduce the visual and noise impacts and produce more power due to higher wind speeds. Nevertheless, the increasing penetration of wind energy, as well as other renewable sources, is still a great concern due to their fluctuating and intermittent behavior. Therefore, in order to cover the mismatch between power generation and load demand, the stochastic nature of renewables has to be mitigated. Among proposed solutions, the integration of energy storage systems in wind power plants is one of the most effective. In this paper, a Hybrid Energy Storage System (HESS) is integrated into an offshore wind turbine generator with the aim of demonstrating the benefits in terms of fluctuation reduction of the active power and voltage waveform frequency, specifically at the Point of Common Coupling (PCC). A MATLAB®/SimPowerSystems model composed of an offshore wind turbine interfaced with the grid through a full-scale back-to-back converter and a flywheel-battery-based HESS connected to the converter DC-link has been developed and compared with the case of storage absence. Simulations were carried out in reference to the wind turbine’s stress conditions and were selected—according to our previous work—in terms of the wind power step. Specifically, the main outcomes of this paper show that HESS integration allows for a reduction in the active power variation, when the wind power step is applied, to about 3% and 4.8%, respectively, for the simulated scenarios, in relation to more than 30% and 42% obtained for the no-storage case. Furthermore, HESS is able to reduce the transient time of the frequency of the three-phase voltage waveform at the PCC by more than 89% for both the investigated cases. Hence, this research demonstrates how HESS, coupled with renewable power plants, can strongly enhance grid safety and stability issues in order to meet the stringent requirements relating to the massive RES penetration expected in the coming years.

The need for environmental protection is pushing to a massive introduction of energy production from renewables. Although wind and solar energy present the most mature technologies for energy generation, wave energy has a huge annual energy potential not exploited yet. Indeed, no leading device for wave energy conversion has already been developed. Hence, the future exploitation of wave energy will be strictly related to a specific infrastructure for power distribution and transmission that has to satisfy high requirements to guarantee grid safety and stability, because of the stochastic nature of this source. To this end, an electrical architecture model, based on a common DC bus topology and including a Hybrid Energy Storage System (HESS) composed by Li-ion battery and flywheel coupled to a wave energy converter, is here presented. In detail, this research work wants to investigate the beneficial effects in terms of voltage and current waveforms frequency and transient behavior at the Point of Common Coupling (PCC) introduced by HESS under specific stressful production conditions. Specifically, in the defined simulation scenarios it is demonstrated that the peak value of the voltage wave frequency at the PCC is reduced by 64% to 80% with a faster stabilization in the case of HESS with respect to storage absence, reaching the set value (50 Hz) in a shorter time (by −10% to −42%). Therefore, HESS integration in wave energy converters can strongly reduce safety and stability issues of the main grid relating to intermittent and fluctuating wave production, significantly increasing the tolerance to the expected increasing share of electricity from renewable energy sources.

Background Cigarette smoke oxidants can cause oxidative inactivation of methionine residues of α 1 -AT, leading to lipid binding, with dyslipidemia, inflammation and endothelial dysfunction, the early stage of atherosclerosis. The aim of the study was to assess the relationship between serum α 1 -AT levels, pulmonary function and lipids in predicting the risk of cardiovascular disease among symptomatic smokers adult subjects in an Out-patient Pulmonology Health Unit. Methods 100 respiratory symptomatic adults mean aged 53.34±9.69 year-old, were grouped in smokers (current and former heavy smokers) (n=64) and nonsmokers (n=36). Demographic, anthropometric, pack-years smoking, serum α 1 -AT, plasma lipid profile {(total cholesterol-TC, low density lipoprotein cholesterol-LDLc, high density lipoprotein cholesterol-HDLc, triglycerides-TG, nonhigh density lipoprotein cholesterol-nonHDLc, very low density lipoprotein cholesterol-VLDL), by which were calculated lipid indices}, were performed. All subjects underwent spirometry according to the international recommendations. Lung function was expressed as forced vital capacity (FVC% predicted), forced expiratory volume in one second (FEV1% predicted), Forced Expiratory volume in 1 second/Forced Vital Capacity ratio (FEV1/FVC% predicted). Cardiovascular disease risk was evaluated through Framingham Risk Score (FRS). Statistical analysis included Spearman correlations tests and one-way ANOVA test. Results α 1 -AT (p=0.008), FEV1 (p=0.008), FVC (p=0.03) was significantly lower, while LDLc/HDLc ratio (p=0.01), Atherogenic Index of Plasma (AIP) (p=0.04), FRS (p=0.003) increased in smokers versus nonsmokers. In smokers, statistically significant correlations were found between α 1 -AT and FVC (r=-0.26;p=0.03); FEV1/FVC ratio (r=0.30;p=0.01); TC (r=0.35;p=0.003); LDLc (r=0.27; p=0.02); nonHDLc (r=0.26;p=0.03). Conclusions α 1 -AT interacts with lipid oxidation pathways, decreasing in smokers. A better understanding of the relationships between smoking, oxidative modifications of α 1 -AT, lung function and, lipid profile can better predict the risk of heart disease. Smoking is a modifiable health risk factor and tobacco cessation may prevent the occurence of cardiovascular diseases.

The modalities of citizen participation in the decision-making process of inter-community structures or associations are formulated as principles, general rules. The democratic deficit of such structures is denounced in many European states. Although the main purpose of these associations is to streamline and increase efficiency of local management to the citizens' benefit, the citizens have no means of determining or influencing the exercise of competencies by the local government/authority. The objective of this topic is to analyze the experience of the associations′ evolution in the European space, predominantly in Romania, based on the raports of European Comittee on Local and Regional Democracy and roumanian legislation. The purpose of this analysis is to draw lessons and recommendations for effective citizen participation in the inter-community. The citizens′ participation represents an issue of concern for many states, and is taken into consideration in the analysis of the services offered.